﻿PT	AU	BA	BE	GP	AF	BF	CA	TI	SO	SE	BS	LA	DT	CT	CY	CL	SP	HO	DE	ID	AB	C1	RP	EM	RI	OI	FU	FX	CR	NR	TC	Z9	U1	U2	PU	PI	PA	SN	EI	BN	J9	JI	PD	PY	VL	IS	PN	SU	SI	MA	BP	EP	AR	DI	D2	EA	PG	WC	SC	GA	UT	PM	OA	HC	HP	DA
J	Yao, HQ; Scornet, D; Jam, M; Herve, C; Potin, P; Correia, LO; Coelho, SM; Cock, JM				Yao, Haiqin; Scornet, Delphine; Jam, Murielle; Herve, Cecile; Potin, Philippe; Oliveira Correia, Lydie; Coelho, Susana M.; Cock, J. Mark			Biochemical characteristics of a diffusible factor that induces gametophyte to sporophyte switching in the brown alga Ectocarpus	JOURNAL OF PHYCOLOGY			English	Article; Early Access						arabinogalactan protein; diffusible factor; Ectocarpus; gametophyte; life cycle; sporophyte		The haploid-diploid life cycle of the filamentous brown alga Ectocarpus involves alternation between two independent and morphologically distinct multicellular generations, the sporophyte and the gametophyte. Deployment of the sporophyte developmental program requires two TALE homeodomain transcription factors OUROBOROS and SAMSARA. In addition, the sporophyte generation has been shown to secrete a diffusible factor that can induce uni-spores to switch from the gametophyte to the sporophyte developmental program. Here, we determine optimal conditions for production, storage, and detection of this diffusible factor and show that it is a heat-resistant, high molecular weight molecule. Based on a combined approach involving proteomic analysis of sporophyte-conditioned medium and the use of biochemical tools to characterize arabinogalactan proteins, we present evidence that sporophyte-conditioned medium contains AGP epitopes and suggest that the diffusible factor may belong to this family of glycoproteins.	[Yao, Haiqin; Scornet, Delphine; Coelho, Susana M.; Cock, J. Mark] UPMC Univ Paris 06, Sorbonne Univ, UMR 8227, Algal Genet Grp,CNRS, Paris, France; [Yao, Haiqin; Scornet, Delphine; Jam, Murielle; Herve, Cecile; Potin, Philippe; Coelho, Susana M.; Cock, J. Mark] Integrat Biol Marine Models, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; [Jam, Murielle; Herve, Cecile] UPMC Univ Paris 06, Sorbonne Univ, CNRS, Marine Glycobiol,UMR 8227, Paris, France; [Potin, Philippe] UPMC Univ Paris 06, Sorbonne Univ, CNRS, Algal Biol & Environm Interact,UMR 8227, Paris, France; [Oliveira Correia, Lydie] Univ Paris Saclay, Micalis Inst, PAPPSO, AgroParisTech,INRA, F-78350 Jouy En Josas, France	Coelho, SM; Cock, JM (corresponding author), UPMC Univ Paris 06, Sorbonne Univ, UMR 8227, Algal Genet Grp,CNRS, Paris, France.; Coelho, SM; Cock, JM (corresponding author), Integrat Biol Marine Models, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.	coelho@sb-roscoff.fr; cock@sb-roscoff.fr		Oliveira Correia, Lydie/0000-0002-8590-250X; POTIN, Philippe/0000-0001-7358-6282	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); European Research CouncilEuropean Research Council (ERC)European Commission [638240]; Sorbonne University; French National Research AgencyFrench National Research Agency (ANR) [ANR-10-BTBR-04-02]; Chinese Scholarship CouncilChina Scholarship Council [201608310119]	This work was supported by the Centre National de la Recherche Scientifique, the European Research Council (grant agreement 638240) and Sorbonne University. Support was also provided by the French National Research Agency via the investment expenditure program Idealg (ANR-10-BTBR-04-02). HY was supported by a grant from the Chinese Scholarship Council (grant number 201608310119).	Acosta-Garcia G, 2004, PLANT CELL, V16, P2614, DOI 10.1105/tpc.104.024588; Arun A, 2019, ELIFE, V8, DOI 10.7554/eLife.43101; Arun A, 2013, NEW PHYTOL, V197, P503, DOI 10.1111/nph.12007; BAUER L, 1959, NATURWISSENSCHAFTEN, V46, P154; Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Brunelle JL, 2014, METHOD ENZYMOL, V541, P151, DOI 10.1016/B978-0-12-420119-4.00012-4; CHEUNG AY, 1995, CELL, V82, P383, DOI 10.1016/0092-8674(95)90427-1; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Coelho SM, 2020, ANNU REV GENET, V54, P71, DOI 10.1146/annurev-genet-030620-093031; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Demesa-Arevalo E, 2013, PLANT CELL, V25, P1274, DOI 10.1105/tpc.112.106237; Dittami SM, 2020, MAR GENOM, V52, DOI 10.1016/j.margen.2020.100740; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dupres V, 2009, NAT CHEM BIOL, V5, P857, DOI 10.1038/nchembio.220; Fischl R, 2016, GLYCOBIOLOGY, V26, P973, DOI 10.1093/glycob/cww040; Fu H, 2007, PLANTA, V226, P1511, DOI 10.1007/s00425-007-0587-y; GANE AM, 1995, CARBOHYD RES, V277, P67, DOI 10.1016/0008-6215(95)00197-2; Hancock CN, 2005, PLANT J, V43, P716, DOI 10.1111/j.1365-313X.2005.02490.x; Herve C, 2016, NEW PHYTOL, V209, P1428, DOI 10.1111/nph.13786; Jiao J, 2017, PLANT PHYSIOL, V173, P354, DOI 10.1104/pp.16.01655; Johnson KL, 2003, PLANT PHYSIOL, V133, P1911, DOI 10.1104/pp.103.031237; KREUGER M, 1993, PLANTA, V189, P243, DOI 10.1007/BF00195083; Lodder AL, 1999, GENETICS, V152, P1487; Lommel M, 2004, MOL CELL BIOL, V24, P46, DOI 10.1128/MCB.24.1.46-57.2004; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Mizukami AG, 2016, CURR BIOL, V26, P1091, DOI 10.1016/j.cub.2016.02.040; Moller I, 2007, PLANT J, V50, P1118, DOI 10.1111/j.1365-313X.2007.03114.x; Motose H, 2004, NATURE, V429, P873, DOI 10.1038/nature02613; Motose H, 2001, PLANT CELL PHYSIOL, V42, P129, DOI 10.1093/pcp/pce014; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Ohsawa S, 2017, MOL MICROBIOL, V104, P349, DOI 10.1111/mmi.13631; Oide S, 2019, ENZYME MICROB TECH, V125, P13, DOI 10.1016/j.enzmictec.2019.02.009; Paulsen BS, 2014, CARBOHYD POLYM, V106, P460, DOI 10.1016/j.carbpol.2014.01.009; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Seifert GJ, 2007, ANNU REV PLANT BIOL, V58, P137, DOI 10.1146/annurev.arplant.58.032806.103801; Tan L, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00140; van Hengel AJ, 2001, PLANT PHYSIOL, V125, P1880, DOI 10.1104/pp.125.4.1880; Wawra S, 2019, NEW PHYTOL, V222, P1493, DOI 10.1111/nph.15711	43	0	0	2	2	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.												10.1111/jpy.13126		MAR 2021	12	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	RB9VA	WOS:000632451700001	33432598				2021-04-07	
J	Rathor, P; Borza, T; Stone, S; Tonon, T; Yurgel, S; Potin, P; Prithiviraj, B				Rathor, Pramod; Borza, Tudor; Stone, Sophia; Tonon, Thierry; Yurgel, Svetlana; Potin, Philippe; Prithiviraj, Balakrishnan			A Novel Protein from Ectocarpus sp. Improves Salinity and High Temperature Stress Tolerance in Arabidopsis thaliana	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES			English	Article						Arabidopsis thaliana; Ectocarpus sp; unknown function protein; transgenic plant; salinity; temperature; abiotic stress tolerance		Brown alga Ectocarpus sp. belongs to Phaeophyceae, a class of macroalgae that evolved complex multicellularity. Ectocarpus sp. is a dominant seaweed in temperate regions, abundant mostly in the intertidal zones, an environment with high levels of abiotic stresses. Previous transcriptomic analysis of Ectocarpus sp. revealed several genes consistently induced by various abiotic stresses; one of these genes is Esi0017_0056, which encodes a protein with unknown function. Bioinformatics analyses indicated that the protein encoded by Esi0017_0056 is soluble and monomeric. The protein was successfully expressed in Escherichia coli, Arabidopsis thaliana and Nicotiana benthamiana. In A. thaliana the gene was expressed under constitutive and stress inducible promoters which led to improved tolerance to high salinity and temperature stresses. The expression of several key abiotic stress-related genes was studied in transgenic and wild type A. thaliana by qPCR. Expression analysis revealed that genes involved in ABA-induced abiotic stress tolerance, K+ homeostasis, and chaperon activities were significantly up-regulated in the transgenic line. This study is the first report in which an unknown function Ectocarpus sp. gene, highly responsive to abiotic stresses, was successfully expressed in A. thaliana, leading to improved tolerance to salt and temperature stress.	[Rathor, Pramod; Borza, Tudor; Yurgel, Svetlana; Prithiviraj, Balakrishnan] Dalhousie Univ, Dept Plant Food & Environm, Truro, NS B2N 5E3, Canada; [Stone, Sophia] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4R2, Canada; [Tonon, Thierry] Univ York, Dept Biol, Ctr Novel Agr Prod, York YO10 5DD, N Yorkshire, England; [Tonon, Thierry; Potin, Philippe] Sorbonne Univ, Integrat Biol Marine Models LBI2M, Stn Biol Roscoff SBR, CNRS,UMR 8227, F-29680 Roscoff, France	Prithiviraj, B (corresponding author), Dalhousie Univ, Dept Plant Food & Environm, Truro, NS B2N 5E3, Canada.	pramod.rathor@dal.ca; Tudor.Borza@dal.ca; s.stone@dal.ca; thierry.tonon@york.ac.uk; syurgel@dal.ca; philippe.potin@sb-roscoff.fr; bprithiviraj@dal.ca	Tonon, Thierry/A-3214-2009	Tonon, Thierry/0000-0002-1454-6018; POTIN, Philippe/0000-0001-7358-6282	NSERC-DG grant [1177546]; MITACS; France-Canada Research Fund (FCRF); NSERC Industrial Postgraduate ScholarshipNatural Sciences and Engineering Research Council of Canada (NSERC); MITACS Globalink Research Award; Agence Nationale de la Recherche via the investment expenditure program IDEALG GrantFrench National Research Agency (ANR) [ANR-10-BTBR-04]	BP's lab was supported by NSERC-DG grant 1177546, MITACS and France-Canada Research Fund (FCRF). PR was supported by an NSERC Industrial Postgraduate Scholarship and a MITACS Globalink Research Award. PP and TT were also supported by the Agence Nationale de la Recherche via the investment expenditure program IDEALG Grant ANR-10-BTBR-04.	Agarwal PK, 2006, PLANT CELL REP, V25, P1263, DOI 10.1007/s00299-006-0204-8; APT KE, 1995, J MOL BIOL, V248, P79, DOI 10.1006/jmbi.1995.0203; Archibald JM, 2012, ADV BOT RES, V64, P87, DOI 10.1016/B978-0-12-391499-6.00003-7; Battacharyya D, 2015, SCI HORTIC-AMSTERDAM, V196, P39, DOI 10.1016/j.scienta.2015.09.012; Buchan DWA, 2019, NUCLEIC ACIDS RES, V47, pW402, DOI 10.1093/nar/gkz297; Cao WH, 2007, PLANT PHYSIOL, V143, P707, DOI 10.1104/pp.106.094292; Clough SJ, 1998, PLANT J, V16, P735, DOI 10.1046/j.1365-313x.1998.00343.x; Cock JM, 2012, ADV BOT RES, V64, P141, DOI 10.1016/B978-0-12-391499-6.00005-0; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Curtis MD, 2003, PLANT PHYSIOL, V133, P462, DOI 10.1104/pp.103.027979; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Draisma S.G., 2003, EVOLUTION TAXONOMY P, P87; Earley KW, 2006, PLANT J, V45, P616, DOI 10.1111/j.1365-313X.2005.02617.x; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Fan D, 2014, SCI HORTIC-AMSTERDAM, V170, P70, DOI 10.1016/j.scienta.2014.02.038; Finkelstein RR, 2002, PLANT CELL, V14, pS15, DOI 10.1105/tpc.010441; Fujita M, 2004, PLANT J, V39, P863, DOI 10.1111/j.1365-313X.2004.02171.x; Fujita Y, 2005, PLANT CELL, V17, P3470, DOI 10.1105/tpc.105.035659; Fujita Y, 2011, J PLANT RES, V124, P509, DOI 10.1007/s10265-011-0412-3; Greener JG, 2019, NAT COMMUN, V10, DOI 10.1038/s41467-019-11994-0; Hattori T, 2002, PLANT CELL PHYSIOL, V43, P136, DOI 10.1093/pcp/pcf014; Iancu CV, 2001, J BIOL CHEM, V276, P42146, DOI 10.1074/jbc.M106294200; Ingram J, 1996, ANNU REV PLANT PHYS, V47, P377, DOI 10.1146/annurev.arplant.47.1.377; Jia FJ, 2014, BIOCHEM BIOPH RES CO, V454, P505, DOI 10.1016/j.bbrc.2014.10.136; Jithesh MN, 2012, HORTSCIENCE, V47, P704, DOI 10.21273/HORTSCI.47.6.704; JONES DT, 1992, COMPUT APPL BIOSCI, V8, P275, DOI 10.1093/bioinformatics/8.3.275; Khan W, 2009, J PLANT GROWTH REGUL, V28, P386, DOI 10.1007/s00344-009-9103-x; Kim JS, 2011, PLANT CELL PHYSIOL, V52, P2136, DOI 10.1093/pcp/pcr143; Kumar S, 2018, MOL BIOL EVOL, V35, P1547, DOI 10.1093/molbev/msy096; LANG V, 1993, PLANT MOL BIOL, V21, P581; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; LEGALL Y, 1993, PROTOPLASMA, V173, P123; Liu YS, 2016, CELL, V165, P535, DOI 10.1016/j.cell.2016.03.014; Maier T, 2009, FEBS LETT, V583, P3966, DOI 10.1016/j.febslet.2009.10.036; Michniewicz Marta, 2015, BMC Res Notes, V8, P63, DOI 10.1186/s13104-015-1010-6; Nakashima K, 2000, PLANT MOL BIOL, V42, P657, DOI 10.1023/A:1006321900483; Nakashima K, 2009, PLANT PHYSIOL, V149, P88, DOI 10.1104/pp.108.129791; NORDIN K, 1993, PLANT MOL BIOL, V21, P641, DOI 10.1007/BF00014547; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Rathor P, 2020, PLANTS-BASEL, V9, DOI 10.3390/plants9111508; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Saibil H, 2013, NAT REV MOL CELL BIO, V14, P630, DOI 10.1038/nrm3658; Sakuma Y, 2006, PLANT CELL, V18, P1292, DOI 10.1105/tpc.105.035881; Sakuma Y, 2002, BIOCHEM BIOPH RES CO, V290, P998, DOI 10.1006/bbrc.2001.6299; Sakuma Y, 2006, P NATL ACAD SCI USA, V103, P18822, DOI 10.1073/pnas.0605639103; Shanmugaraj B, 2020, PLANTS-BASEL, V9, DOI 10.3390/plants9070842; Shevchenko A, 2006, NAT PROTOC, V1, P2856, DOI 10.1038/nprot.2006.468; Shi HZ, 2003, NAT BIOTECHNOL, V21, P81, DOI 10.1038/nbt766; Shinozaki K, 2003, CURR OPIN PLANT BIOL, V6, P410, DOI 10.1016/S1369-5266(03)00092-X; Sparkes IA, 2006, NAT PROTOC, V1, P2019, DOI 10.1038/nprot.2006.286; Suzuki T., 2020, REF MODUL CHEM MOL S, DOI [10.1016/B978-0-12-409547-2.14947-9, DOI 10.1016/B978-0-12-409547-2.14947-9]; Takahashi S, 2000, PLANT CELL PHYSIOL, V41, P898, DOI 10.1093/pcp/pcd010; Veeranagamallaiah G, 2011, J PLANT PHYSIOL, V168, P671, DOI 10.1016/j.jplph.2010.09.007; Vogel C, 2012, NAT REV GENET, V13, P227, DOI 10.1038/nrg3185; Wang JY, 2020, BIOINFORMATICS, V36, P131, DOI 10.1093/bioinformatics/btz502; Wang WX, 2004, TRENDS PLANT SCI, V9, P244, DOI 10.1016/j.tplants.2004.03.006; Waterhouse A, 2018, NUCLEIC ACIDS RES, V46, pW296, DOI 10.1093/nar/gky427; Wise MJ, 2003, BMC BIOINFORMATICS, V4, DOI 10.1186/1471-2105-4-52; Yamaguchi-Shinozaki K, 2005, TRENDS PLANT SCI, V10, P88, DOI 10.1016/j.tplants.2004.12.012; YAMAGUCHISHINOZAKI K, 1993, MOL GEN GENET, V236, P331, DOI 10.1007/BF00277130; YAMAGUCHISHINOZAKI K, 1994, PLANT CELL, V6, P251, DOI 10.1105/tpc.6.2.251; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Yoshida T, 2010, PLANT J, V61, P672, DOI 10.1111/j.1365-313X.2009.04092.x; Zhu JK, 2016, CELL, V167, P313, DOI 10.1016/j.cell.2016.08.029; Zhu JK, 2002, ANNU REV PLANT BIOL, V53, P247, DOI 10.1146/annurev.arplant.53.091401.143329	65	0	0	0	0	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		1422-0067		INT J MOL SCI	Int. J. Mol. Sci.	FEB	2021	22	4							1971	10.3390/ijms22041971			20	Biochemistry & Molecular Biology; Chemistry, Multidisciplinary	Biochemistry & Molecular Biology; Chemistry	QP3SX	WOS:000623758400001	33671243	DOAJ Gold, Green Published, Green Accepted			2021-04-07	
J	Bourdareau, S; Tirichine, L; Lombard, B; Loew, D; Scornet, D; Wu, Y; Coelho, SM; Cock, JM				Bourdareau, Simon; Tirichine, Leila; Lombard, Berangere; Loew, Damarys; Scornet, Delphine; Wu, Yue; Coelho, Susana M.; Cock, J. Mark			Histone modifications during the life cycle of the brown alga Ectocarpus	GENOME BIOLOGY			English	Article						Brown algae; ChIP-seq; Chromatin; Ectocarpus; Gametophyte; Histone modification; Life cycle; Multicellularity; Polycomb complex; Sporophyte	REPRESSIVE COMPLEX 2; ENDOSPERM DEVELOPMENT; INTEGRATIVE ANALYSIS; SEED DEVELOPMENT; ACTIVE GENES; DRAFT GENOME; POLYCOMB; METHYLATION; H3; TRANSCRIPTION	BackgroundBrown algae evolved complex multicellularity independently of the animal and land plant lineages and are the third most developmentally complex phylogenetic group on the planet. An understanding of developmental processes in this group is expected to provide important insights into the evolutionary events necessary for the emergence of complex multicellularity. Here, we focus on mechanisms of epigenetic regulation involving post-translational modifications of histone proteins.ResultsA total of 47 histone post-translational modifications are identified, including a novel mark H2AZR38me1, but Ectocarpus lacks both H3K27me3 and the major polycomb complexes. ChIP-seq identifies modifications associated with transcription start sites and gene bodies of active genes and with transposons. H3K79me2 exhibits an unusual pattern, often marking large genomic regions spanning several genes. Transcription start sites of closely spaced, divergently transcribed gene pairs share a common nucleosome-depleted region and exhibit shared histone modification peaks. Overall, patterns of histone modifications are stable through the life cycle. Analysis of histone modifications at generation-biased genes identifies a correlation between the presence of specific chromatin marks and the level of gene expression.ConclusionsThe overview of histone post-translational modifications in the brown alga presented here will provide a foundation for future studies aimed at understanding the role of chromatin modifications in the regulation of brown algal genomes.	[Bourdareau, Simon; Scornet, Delphine; Coelho, Susana M.; Cock, J. Mark] UPMC Univ Paris 06, CNRS, Sorbonne Univ,Stn Biol Roscoff, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, CS 90074, F-29688 Roscoff, France; [Tirichine, Leila; Wu, Yue] Univ Nantes, CNRS, UFIP, UMR 6286, F-44000 Nantes, France; [Lombard, Berangere; Loew, Damarys] PSL Res Univ, Inst Curie, Ctr Rech, Lab Spectrometrie Masse Proteom, 26 Rue Ulm, F-75248 Paris 05, France; [Coelho, Susana M.] Max Planck Inst Dev Biol, Max Planck Ring 5, D-72076 Tubingen, Germany	Coelho, SM; Cock, JM (corresponding author), UPMC Univ Paris 06, CNRS, Sorbonne Univ,Stn Biol Roscoff, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, CS 90074, F-29688 Roscoff, France.	susana.coelho@tuebingen.mpg.de; cock@sb-roscoff.fr			Centre National de la Recherche Scientifique, Sorbonne University; Agence Nationale de la Recherche project EpicycleFrench National Research Agency (ANR) [ANR-19-CE20-0028-01]; Agence Nationale de la Recherche project IdealgFrench National Research Agency (ANR) [ANR-10BTBR-04-01]; European Research CouncilEuropean Research Council (ERC)European Commission [638240]; Region Ile-de-FranceRegion Ile-de-France; Fondation pour la Recherche MedicaleFondation pour la Recherche Medicale	This work was supported by the Centre National de la Recherche Scientifique, Sorbonne University (including a PhD grant for SB), the Agence Nationale de la Recherche projects Epicycle and Idealg (ANR-19-CE20-0028-01 and ANR-10BTBR-04-01, respectively) and the European Research Council (grant agreement 638240). DL acknowledges support from the Region Ile-de-France and the Fondation pour la Recherche Medicale.	Abeel T, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gkr995; Akkers RC, 2009, DEV CELL, V17, P425, DOI 10.1016/j.devcel.2009.08.005; Allshire RC, 2018, NAT REV MOL CELL BIO, V19, P229, DOI 10.1038/nrm.2017.119; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Andrews S., 2016, FASTQC QUALITY CONTR; Arun A, 2019, ELIFE, V8, DOI 10.7554/eLife.43101; Balakrishnan L, 2010, CRIT REV BIOCHEM MOL, V45, P440, DOI 10.3109/10409238.2010.504700; Barnes CE, 2019, ESSAYS BIOCHEM, V63, P97, DOI 10.1042/EBC20180061; Barski A, 2007, CELL, V129, P823, DOI 10.1016/j.cell.2007.05.009; Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Bourdareau S, 2020, GENE EXPR OMN; Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; Bu J, 2018, LEUKEMIA, V32, P890, DOI 10.1038/leu.2017.339; Charron JBF, 2009, PLANT CELL, V21, P3732, DOI 10.1105/tpc.109.066845; Chaudhury AM, 1997, P NATL ACAD SCI USA, V94, P4223, DOI 10.1073/pnas.94.8.4223; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2011, J EXP BOT, V62, P2425, DOI 10.1093/jxb/err117; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Sanchez MD, 2009, P NATL ACAD SCI USA, V106, P2065, DOI 10.1073/pnas.0811093106; Dong XJ, 2012, GENOME BIOL, V13, DOI 10.1186/gb-2012-13-9-r53; Du JM, 2012, CELL, V151, P167, DOI 10.1016/j.cell.2012.07.034; Godfroy O, 2017, PLANT CELL, V29, P3102, DOI 10.1105/tpc.17.00440; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Guenther MG, 2007, CELL, V130, P77, DOI 10.1016/j.cell.2007.05.042; Guitton AE, 2004, DEVELOPMENT, V131, P2971, DOI 10.1242/dev.01168; Guitton AE, 2005, CURR BIOL, V15, P750, DOI 10.1016/j.cub.2005.02.066; Hansen P, 2016, BMC GENOMICS, V17, DOI 10.1186/s12864-016-3164-6; Hoffmann RD, 2013, MOL PLANT, V6, P1176, DOI 10.1093/mp/sst100; Huff JT, 2010, NAT STRUCT MOL BIOL, V17, P1495, DOI 10.1038/nsmb.1924; Iyer LM, 2008, INT J PARASITOL, V38, P1, DOI 10.1016/j.ijpara.2007.07.018; Johnson L, 2004, NUCLEIC ACIDS RES, V32, P6511, DOI 10.1093/nar/gkh992; Jung CH, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00328; Jurkowska RZ, 2017, NAT COMMUN, V8, DOI 10.1038/s41467-017-02259-9; Karlic R, 2010, P NATL ACAD SCI USA, V107, P2926, DOI 10.1073/pnas.0909344107; Karmodiya K, 2012, BMC GENOMICS, V13, DOI 10.1186/1471-2164-13-424; Kim D, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-4-r36; Kohler C, 2003, EMBO J, V22, P4804, DOI 10.1093/emboj/cdg444; Kotlinski M, 2017, PLANT PHYSIOL, V174, P27, DOI 10.1104/pp.16.00214; Krogan NJ, 2003, MOL CELL BIOL, V23, P4207, DOI 10.1128/MCB.23.12.4207-4218.2003; Krzywinski M, 2009, GENOME RES, V19, P1639, DOI 10.1101/gr.092759.109; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Lawrence M, 2016, TRENDS GENET, V32, P42, DOI 10.1016/j.tig.2015.10.007; Lerdrup M, 2016, NAT STRUCT MOL BIOL, V23, P349, DOI 10.1038/nsmb.3180; Li TB, 2018, GENOME MED, V10, DOI 10.1186/s13073-018-0538-1; Liao Y, 2014, BIOINFORMATICS, V30, P923, DOI 10.1093/bioinformatics/btt656; Lin X, 2012, PLANT METHODS, V8, DOI 10.1186/1746-4811-8-48; Lipinska AP, 2019, GENOME BIOL, V20, DOI 10.1186/s13059-019-1630-6; Liu YH, 2019, EPIGENET CHROMATIN, V12, DOI 10.1186/s13072-019-0285-6; Loew D, 2020, EPIGENETIC MODIFICAT; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; Luco RF, 2010, SCIENCE, V327, P996, DOI 10.1126/science.1184208; Macaisne N, 2017, DEVELOPMENT, V144, P409, DOI 10.1242/dev.141523; Mikkelsen TS, 2007, NATURE, V448, P553, DOI 10.1038/nature06008; Mosquna A, 2009, DEVELOPMENT, V136, P2433, DOI 10.1242/dev.035048; Mozgova I, 2015, ANNU REV PLANT BIOL, V66, P269, DOI 10.1146/annurev-arplant-043014-115627; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Ng HH, 2003, P NATL ACAD SCI USA, V100, P1820, DOI 10.1073/pnas.0437846100; Nishitsuji K, 2019, SCI REP-UK, V9, DOI 10.1038/s41598-019-40955-2; Nishitsuji K, 2016, DNA RES, V23, P561, DOI 10.1093/dnares/dsw039; Ohad N, 1999, PLANT CELL, V11, P407, DOI 10.1105/tpc.11.3.407; Ohad N, 1996, P NATL ACAD SCI USA, V93, P5319, DOI 10.1073/pnas.93.11.5319; Okano Y, 2009, P NATL ACAD SCI USA, V106, P16321, DOI 10.1073/pnas.0906997106; Pajoro A, 2017, GENOME BIOL, V18, DOI 10.1186/s13059-017-1235-x; Perez-Riverol Y, 2019, NUCLEIC ACIDS RES, V47, pD442, DOI 10.1093/nar/gky1106; Peters AHFM, 2003, MOL CELL, V12, P1577, DOI 10.1016/S1097-2765(03)00477-5; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Philpott A, 2000, SEMIN CELL DEV BIOL, V11, P7, DOI 10.1006/scdb.1999.0346; Quinlan AR, 2010, BIOINFORMATICS, V26, P841, DOI 10.1093/bioinformatics/btq033; Ramirez F, 2016, NUCLEIC ACIDS RES, V44, pW160, DOI 10.1093/nar/gkw257; Rice JC, 2003, MOL CELL, V12, P1591, DOI 10.1016/S1097-2765(03)00479-9; Roudier F, 2011, EMBO J, V30, P1928, DOI 10.1038/emboj.2011.103; Santos-Rosa H, 2002, NATURE, V419, P407, DOI 10.1038/nature01080; Schubeler D, 2004, GENE DEV, V18, P1263, DOI 10.1101/gad.1198204; Schuettengruber B, 2017, CELL, V171, P34, DOI 10.1016/j.cell.2017.08.002; Shaver S, 2010, EPIGENETICS-US, V5, P301, DOI 10.4161/epi.5.4.11608; Steger DJ, 2008, MOL CELL BIOL, V28, P2825, DOI 10.1128/MCB.02076-07; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Talbert PB, 2012, EPIGENET CHROMATIN, V5, DOI 10.1186/1756-8935-5-7; Tarver JE, 2015, NUCLEIC ACIDS RES, V43, P6384, DOI 10.1093/nar/gkv578; Tirichine L, 2014, MAR GENOM, V13, P21, DOI 10.1016/j.margen.2013.11.006; Veluchamy A, 2015, GENOME BIOL, V16, DOI 10.1186/s13059-015-0671-8; Waterborg JH, 2012, BIOCHEM CELL BIOL, V90, P79, DOI [10.1139/O11-036, 10.1139/o11-036]; Wu ZF, 2019, PLANT CELL PHYSIOL, V60, P1471, DOI 10.1093/pcp/pcz051; Xu J, 2018, BMC GENOMICS, V19, DOI 10.1186/s12864-018-4886-4; Yang HC, 2014, CURR BIOL, V24, P1793, DOI 10.1016/j.cub.2014.06.047; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zhang F, 2016, NAT COMMUN, V7, DOI 10.1038/ncomms13018; Zhang KL, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0001210; Zhang XY, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r62; Zhang Y, 2008, GENOME BIOL, V9, DOI 10.1186/gb-2008-9-9-r137; Zhao X, 2019, H3K27ME3 NATURAL VAR, V2019, P1226888800	94	0	0	0	0	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1474-760X			GENOME BIOL	Genome Biol.	JAN 4	2021	22	1							12	10.1186/s13059-020-02216-8			27	Biotechnology & Applied Microbiology; Genetics & Heredity	Biotechnology & Applied Microbiology; Genetics & Heredity	PR2NX	WOS:000607079200012	33397407	DOAJ Gold, Green Published			2021-04-07	
J	Mendoza-Gonzalez, AC; Mateo-Cid, LE; Ortega-Murillo, MD; Zurita-Valencia, L; Sanchez-Heredia, JD; Hernandez-Casas, CM				Mendoza-Gonzalez, Angela Catalina; Mateo-Cid, Luz Elena; Ortega-Murillo, Maria del Rosario; Zurita-Valencia, Leslie; Sanchez-Heredia, Juan Diego; Hernandez-Casas, Cynthia Mariana			New records and updated list of brown algae (Phaeophyceae) from the coast of Michoacan, Mexico	REVISTA DE BIOLOGIA MARINA Y OCEANOGRAFIA			Spanish	Article						Phaeophyceae; inventory; seasonality; species richness	BENTHIC MARINE-ALGAE; DICTYOTALES; POPULATIONS	Brown algae (Phaeophyceae) recorded in the literature and specimens at herbaria from the littoral of Michoacan were extensively reviewed. Additionally, these data were supplemented with samplings in 11 localities from 2006 to 2014. A total of 52 infrageneric taxa of brown algae (49 species and 3 varieties) were determined; from these, 27 were new records for Michoacan, being Streblonema anomalum and Ectocarpus taoniae new records for the Pacific coast of Mexico. The highest number of species was recorded for La Majahuita (27), while the lowest number of taxa was in San Telmo (2). The family Dictyotaceae was the best represented (15 taxa), followed by Chordariaceae and Ectocarpaceae (5 each one). From the previous 25 records in this area, the presence of 12 taxa was confirmed, so the number of Phaeophyceae from this coast increased to 52 taxa. The floristic list is accompanied by data of presence, seasonality, reproduction, habitat and bibliographic references. Species diversity was compared between the dry and rainy seasons during 2006 to 2014, the greatest diversity was found during the dry season. The coast of Michoacan has the highest specific richness of Phaeophyceae when comparing to Nayarit, Colima, Jalisco, Guerrero, Oaxaca and Chiapas.	[Mendoza-Gonzalez, Angela Catalina; Mateo-Cid, Luz Elena; Hernandez-Casas, Cynthia Mariana] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Bot, Carpio & Plan Ayala S-N, Mexico City 11340, DF, Mexico; [Ortega-Murillo, Maria del Rosario; Zurita-Valencia, Leslie; Sanchez-Heredia, Juan Diego] Univ Michoacana, Fac Biol, Gral Francisco J Mugica S-N,Ciudad, Morelia 58030, Michoacan, Mexico	Mendoza-Gonzalez, AC (corresponding author), Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Bot, Carpio & Plan Ayala S-N, Mexico City 11340, DF, Mexico.	am7124@gmail.com					AVILA-ORTIZ A, 2005, MONOGRAFIAS FICOLOGI, V2, P139; BASTIDAZAVALA JR, 2013, CHECK LIST, V9, P329; Brand DD, 2013, ESTUDIO COSTERO SURO; Mendoza-Gonzalez AC, 2018, REV MEX BIODIVERS, V89, P971, DOI 10.22201/ib.20078706e.2018.4.2604; Mendoza-Gonzalez AC, 2011, REV MEX BIODIVERS, V82, P19; Chavez-Barrera ML, 1980, ANALES ESCUELA NACL, V23, P45; Diaz-Martin MA, 2000, B MAR SCI, V66, P279; Mateo-Cid LE, 2013, ACTA BOT MEX, V104, P53, DOI 10.21829/abm104.2013.57; Mateo-Cid LE, 2012, REV MEX BIODIVERS, V83, P905, DOI 10.7550/rmb.28104; Fletcher R.L., 1987, BRIT MUSEUM NATURA 1, V3, p[i, 1]; Garcia E., 1983, MODIFICACIONES SISTE; Guiry M. D., 2019, WORLD WIDE ELECT PUB; Kim HS, 2010, ALGAL FLORA KOREA, V2, P5; La Barre S, 2010, MAR DRUGS, V8, P988, DOI 10.3390/md8040988; Lalli CM, 1997, HIST SEXUALITY; Leon Hilda, 1993, Hydrobiologia, V260-261, P197, DOI 10.1007/BF00049020; Leon-Alvarez D., 1993, BIODIVERSIDAD MARINA, P456; Liu XJ, 2017, BOT MAR, V60, P89, DOI 10.1515/bot-2016-0091; Lugo AE, 2000, SCI TOTAL ENVIRON, V262, P243, DOI 10.1016/S0048-9697(00)00526-X; Mateo-Cid L, 1991, ACTA BOT MEX, V13, P9; Mateo-Cid L, 1992, ACTA BOT MEX, V20, P13; Mateo-Cid L.E., 2001, ANALES ESCUELA NACL, V47, P11; Mateo-Cid LE, 2019, PAK J BOT, V51, P1; Mendoza-Gonzalez A.C., 1996, POLIBOTANICA, V2, P61; MENDOZA-GONZALEZ A. C., 2000, POLIBOTANICA, V11, P21; Montanes MA, 2006, BOT MAR, V49, P406, DOI 10.1515/BOT.2006.052; Montecinos AE, 2017, J PHYCOL, V53, P17, DOI 10.1111/jpy.12452; Norris J.N., 2010, MARINE ALGAE NO GULF; Pedroche FF, 2008, CATALOGO ALGAS MARIN; PHILLIPS JA, 1988, BOT MAR, V31, P437, DOI 10.1515/botm.1988.31.5.437; Santelices B., 1977, ECOLOGIA ALGAS MARIN; Schneider CW, 1991, SEAWEEDS SE US HATTE; SEMAR, 2002, ATL DIN COST REP MEX; Senties A., 1990, B SOC BOT MEX, V50, P19, DOI DOI 10.17129/B0TSCI.1375; Setchell W. A., 1924, P CALIF ACAD SCI, V4, P695; Setchell WA, 1922, U CALIFORNIA PUBLICA, V7, P403; Stout Ilsa, 1993, Anales del Instituto de Biologia Universidad Nacional Autonoma de Mexico Serie Botanica, V64, P1; Taylor W.R., 1945, A HANCOCK PACIFIC EX, V12, P1; Taylor WR, 1960, MARINE ALGAE E TROPI; Thiers B., 2020, INDEX HERBARIORUM GL; Tronholm A, 2008, BOT MAR, V51, P132, DOI 10.1515/BOT.2008.017; Wynne MJ, 2017, NOVA HEDWIG BEIH, V145, P7	42	0	0	0	0	UNIV VALPARAISO	VINA DEL MAR	FACULTAD CIENCIAS MAR RECURSOS NATURALES, CASILLA 5080 - RENACA, VINA DEL MAR, 00000, CHILE	0717-3326	0718-1957		REV BIOL MAR OCEANOG	Rev. Biol. Mar. Oceanogr.	DEC	2020	55	3					202	216		10.22370/rbmo.2020.55.3.2583			15	Marine & Freshwater Biology; Oceanography	Marine & Freshwater Biology; Oceanography	QB5ZH	WOS:000614217500005		Other Gold			2021-04-07	
J	Kang, J; Park, JS; Jung, SW; Kim, HJ; Joo, HM; Kang, D; Seo, H; Kim, S; Jang, MC; Lee, KW; Oh, SJ; Lee, S; Lee, TK				Kang, Junsu; Park, Joon Sang; Jung, Seung Won; Kim, Hyun-Jung; Joo, Hyoung Min; Kang, Donhyug; Seo, Hyojeong; Kim, Sunju; Jang, Min-Chul; Lee, Kyun-Woo; Jin Oh, Seok; Lee, Sukchan; Lee, Taek-Kyun			Zooming on dynamics of marine microbial communities in the phycosphere of Akashiwo sanguinea (Dinophyta) blooms	MOLECULAR ECOLOGY			English	Article							DINOFLAGELLATE BLOOMS; SKELETONEMA-COSTATUM; BIOLOGICAL-CONTROL; ALGAL BLOOMS; RED TIDE; VIRUSES; PHYTOPLANKTON; PARASITISM; POLYSACCHARIDES; DIVERSITY	Characterizing ecological relationships between viruses, bacteria and phytoplankton in the ocean is critical to understanding the ecosystem; however, these relationships are infrequently investigated together. To understand the dynamics of microbial communities and environmental factors in harmful algal blooms (HABs), we examined the environmental factors and microbial communities during Akashiwo sanguinea HABs in the Jangmok coastal waters of South Korea by metagenomics. Specific bacterial species showed complex synergistic and antagonistic relationships with the A. sanguinea bloom. The endoparasitic dinoflagellate Amoebophrya sp. 1 controlled the bloom dynamics and correlated with HAB decline. Among nucleocytoplasmic large DNA viruses (NCLDVs), two Pandoraviruses and six Phycodnaviruses were strongly and positively correlated with the HABs. Operational taxonomic units of microbial communities and environmental factors associated with A. sanguinea were visualized by network analysis: A. sanguinea-Amoebophrya sp. 1 (r = .59, time lag: 2 days) and A. sanguinea-Ectocarpus siliculosus virus 1 in Phycodnaviridae (0.50, 4 days) relationships showed close associations. The relationship between A. sanguinea and dissolved inorganic phosphorus relationship also showed a very close correlation (0.74, 0 day). Microbial communities and the environment changed dynamically during the A. sanguinea bloom, and the rapid turnover of microorganisms responded to ecological interactions. A. sanguinea bloom dramatically changes the environments by exuding dissolved carbohydrates via autotrophic processes, followed by changes in microbial communities involving host-specific viruses, bacteria and parasitoids. Thus, the microbial communities in HAB are composed of various organisms that interact in a complex manner.	[Kang, Junsu; Park, Joon Sang; Jung, Seung Won; Kim, Hyun-Jung] Korea Inst Ocean Sci & Technol, Lib Marine Samples, Geoje 53201, South Korea; [Kang, Junsu; Seo, Hyojeong; Kim, Sunju; Jin Oh, Seok] Pukyong Natl Univ, Dept Oceanog, Busan, South Korea; [Joo, Hyoung Min] Korea Polar Res Inst, Div Polar Ocean Sci, Incheon, South Korea; [Kang, Donhyug] Korea Inst Ocean Sci & Technol, Maritime Secur Res Ctr, Busan, South Korea; [Jang, Min-Chul] Korea Inst Ocean Sci & Technol, Ballast Water Res Ctr, Geoje, South Korea; [Lee, Kyun-Woo] Korea Inst Ocean Sci & Technol, Marine Biotechnol Res Ctr, Busan, South Korea; [Lee, Sukchan] Sungkyunkwan Univ, Dept Genet Engn, Suwon, South Korea; [Lee, Taek-Kyun] Korea Inst Ocean Sci & Technol, Risk Assessment Res Ctr, Geoje 53201, South Korea	Jung, SW (corresponding author), Korea Inst Ocean Sci & Technol, Lib Marine Samples, Geoje 53201, South Korea.; Lee, TK (corresponding author), Korea Inst Ocean Sci & Technol, Risk Assessment Res Ctr, Geoje 53201, South Korea.	diatoms@kiost.ac.kr; tklee@kiost.ac.kr	Jung, Seung Won/L-9467-2016	Jung, Seung Won/0000-0002-7473-7924; Park, Joon Sang/0000-0003-1945-2210	National Research Foundation (NRF) - Ministry of Science and ICT (MSIT) [NRF-2020R1A2C2005970, NRF-2017M3A9E4072753]	The stored gDNA samples and fixed phytoplankton samples were obtained from the Library of Marine Samples of Korea Institute of Ocean Science & Technology (KIOST), South Korea. This research was supported by the National Research Foundation (NRF) funded by the Ministry of Science and ICT (MSIT) (NRF-2020R1A2C2005970 and NRF-2017M3A9E4072753).	ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Anderson DM, 1997, NATURE, V388, P513, DOI 10.1038/41415; Andrew, 2010, FASTQC QUALITY CONTR; Arrigo KR, 2005, NATURE, V437, P349, DOI 10.1038/nature04159; Assenov Y, 2008, BIOINFORMATICS, V24, P282, DOI 10.1093/bioinformatics/btm554; Azam F, 2007, NAT REV MICROBIOL, V5, P782, DOI 10.1038/nrmicro1747; Bankevich A, 2012, J COMPUT BIOL, V19, P455, DOI 10.1089/cmb.2012.0021; Bellec L, 2014, BMC EVOL BIOL, V14, DOI 10.1186/1471-2148-14-59; Borsheim KY, 1999, MAR CHEM, V63, P255, DOI 10.1016/S0304-4203(98)00066-8; Caporaso JG, 2010, NAT METHODS, V7, P335, DOI 10.1038/nmeth.f.303; Chambouvet A, 2008, SCIENCE, V322, P1254, DOI 10.1126/science.1164387; Chen TT, 2018, J EUKARYOT MICROBIOL, V65, P448, DOI 10.1111/jeu.12489; Chen WH, 1996, J PLANKTON RES, V18, P1521, DOI 10.1093/plankt/18.9.1521; CLARKE KR, 1993, AUST J ECOL, V18, P117, DOI 10.1111/j.1442-9993.1993.tb00438.x; Claverie JM, 2018, VIRUSES-BASEL, V10, DOI 10.3390/v10090506; Coats DW, 2002, J PHYCOL, V38, P520, DOI 10.1046/j.1529-8817.2002.t01-1-01200.x; Coats DW, 1996, AQUAT MICROB ECOL, V11, P1, DOI 10.3354/ame011001; Colson P, 2013, ARCH VIROL, V158, P2517, DOI 10.1007/s00705-013-1768-6; Croft MT, 2005, NATURE, V438, P90, DOI 10.1038/nature04056; Du XN, 2011, HARMFUL ALGAE, V10, P784, DOI 10.1016/j.hal.2011.06.011; Eiler A, 2012, ISME J, V6, P330, DOI 10.1038/ismej.2011.113; Fajon C, 1999, FEMS MICROBIOL ECOL, V29, P351, DOI 10.1111/j.1574-6941.1999.tb00626.x; Flaviani F, 2017, VIRUSES-BASEL, V9, DOI 10.3390/v9030047; Fuhrman JA, 1999, NATURE, V399, P541, DOI 10.1038/21119; Fuhrman JA, 2008, AQUAT MICROB ECOL, V53, P69, DOI 10.3354/ame01222; Hwang J, 2018, MAR POLLUT BULL, V137, P449, DOI 10.1016/j.marpolbul.2018.10.053; Jessup DA, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0004550; Jung SW, 2008, J APPL MICROBIOL, V105, P186, DOI 10.1111/j.1365-2672.2008.03733.x; Jung SW, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-25345-4; Kim HJ, 2016, MAR POLLUT BULL, V106, P139, DOI 10.1016/j.marpolbul.2016.03.015; Legendre M, 2018, NAT COMMUN, V9, DOI 10.1038/s41467-018-04698-4; Li W., 2016, BIORXIV PREPRINT; Lima-Mendez G, 2015, SCIENCE, V348, DOI 10.1126/science.1262073; Magoc T, 2011, BIOINFORMATICS, V27, P2957, DOI 10.1093/bioinformatics/btr507; Mayali X, 2004, J EUKARYOT MICROBIOL, V51, P139, DOI 10.1111/j.1550-7408.2004.tb00538.x; Mazzillo FFM, 2011, HARMFUL ALGAE, V10, P763, DOI 10.1016/j.hal.2011.06.009; Montagnes DJS, 2008, AQUAT MICROB ECOL, V53, P211, DOI 10.3354/ame01245; MYKLESTAD SM, 1995, SCI TOTAL ENVIRON, V165, P155, DOI 10.1016/0048-9697(95)04549-G; Nagasaki K, 1998, AQUAT MICROB ECOL, V14, P109, DOI 10.3354/ame014109; Naviner M, 1999, AQUACULTURE, V174, P15, DOI 10.1016/S0044-8486(98)00513-4; Oksanen J., 2019, PACKAGE VEGAN COMMUN; PAKULSKI JD, 1994, LIMNOL OCEANOGR, V39, P930, DOI 10.4319/lo.1994.39.4.0930; Paradis E., 2019, PACKAGE APE ANAL PHY; Penna A, 1999, J PLANKTON RES, V21, P1681, DOI 10.1093/plankt/21.9.1681; Philippe N, 2013, SCIENCE, V341, P281, DOI 10.1126/science.1239181; PORTER KG, 1980, LIMNOL OCEANOGR, V25, P943, DOI 10.4319/lo.1980.25.5.0943; Reis JA, 2012, INT REV HYDROBIOL, V97, P389, DOI 10.1002/iroh.201101457; Schloss PD, 2009, APPL ENVIRON MICROB, V75, P7537, DOI 10.1128/AEM.01541-09; Schulz F, 2017, SCIENCE, V356, P82, DOI 10.1126/science.aal4657; Shannon P, 2003, GENOME RES, V13, P2498, DOI 10.1101/gr.1239303; Silveira CB, 2016, NPJ BIOFILMS MICROBI, V2, DOI 10.1038/npjbiofilms.2016.10; Smayda TJ, 1997, LIMNOL OCEANOGR, V42, P1137, DOI 10.4319/lo.1997.42.5_part_2.1137; Urbani R, 2005, SCI TOTAL ENVIRON, V353, P300, DOI 10.1016/j.scitotenv.2005.09.026; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Wickham H., 2020, PACKAGE GGPLOT2 CREA; Winter C, 2010, MICROBIOL MOL BIOL R, V74, P42, DOI 10.1128/MMBR.00034-09; Worden AZ, 2015, SCIENCE, V347, DOI 10.1126/science.1257594; Xia LC, 2013, BIOINFORMATICS, V29, P230, DOI 10.1093/bioinformatics/bts668; Xia LC, 2011, BMC SYST BIOL, V5, DOI 10.1186/1752-0509-5-S2-S15; Yang CY, 2016, SCI REP-UK, V6, DOI 10.1038/srep34645; Yang CY, 2012, HARMFUL ALGAE, V20, P132, DOI 10.1016/j.hal.2012.09.002; Yutin N, 2013, BIOL DIRECT, V8, DOI 10.1186/1745-6150-8-25; Zhou J, 2018, FRONT MICROBIOL, V9, DOI 10.3389/fmicb.2018.01201	63	0	0	16	16	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0962-1083	1365-294X		MOL ECOL	Mol. Ecol.	JAN	2021	30	1					207	221		10.1111/mec.15714		NOV 2020	15	Biochemistry & Molecular Biology; Ecology; Evolutionary Biology	Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology	PK7WX	WOS:000589083700001	33113287	Other Gold, Green Published			2021-04-07	
J	Burgunter-Delamare, B; Boyen, C; Dittami, SM				Burgunter-Delamare, Bertille; Boyen, Catherine; Dittami, Simon M.			Effect of essential oil- and iodine treatments on the bacterial microbiota of the brown alga Ectocarpus siliculosus	JOURNAL OF APPLIED PHYCOLOGY			English	Article						Antibiotics; Essential oils; Povidone-iodine; Brown algae; Microbiome; Metabarcoding	PLANT ESSENTIAL OILS; TEA TREE OIL; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL ACTIVITY; STAPHYLOCOCCUS-AUREUS; CHEMICAL-COMPOSITION; MICROFLORA; PHAEOPHYTA; MECHANISM; THYMOL	Macroalgae live in tight association with bacterial communities, which impact most aspects of their biology. Clean, ideally axenic, algal starting material is required to control and study these interactions. Antibiotics are routinely used to generate clean tissue; however, bacterial resistance to antibiotics is increasingly widespread and sometimes related to the emergence of potentially pathogenic, multi-resistant strains. In this study, we explore the suitability of two alternative treatments for use with algal cultures: essential oils (EOs; thyme, oregano and eucalyptus) and povidone-iodine. The impact of these treatments on bacterial communities was assessed by bacterial cell counts, inhibition diameter experiments and 16S-metabarcoding. Our data show that thyme and oregano essential oils (50% solution in DMSO, 15 h incubation) efficiently reduced the bacterial load of algae without introducing compositional biases, but they did not eliminate all bacteria. Povidone-iodine (2% and 5% solution in artificial seawater, 10 min incubation) both reduced and changed the alga-associated bacterial community, similar to the antibiotic treatment. The proposed EO- and povidone-iodine protocols are thus promising alternatives when only a reduction of bacterial abundance is necessary and where the phenomena of antibiotic resistance are likely to arise.	[Burgunter-Delamare, Bertille; Boyen, Catherine; Dittami, Simon M.] Sorbonne Univ, CNRS, Integrat Biol Marine Models LBI2M, Stn Biol Roscoff, F-29680 Roscoff, France	Burgunter-Delamare, B; Dittami, SM (corresponding author), Sorbonne Univ, CNRS, Integrat Biol Marine Models LBI2M, Stn Biol Roscoff, F-29680 Roscoff, France.	bertille.burgunter-delamare@sb-roscoff.fr; simon.dittami@sb-roscoff.fr			ANR project IDEALGFrench National Research Agency (ANR) [ANR-10-BTBR-04]; CNRSCentre National de la Recherche Scientifique (CNRS)European Commission; Brittany region (Project HOSALA); Sorbonne University [ED227]	This work was funded partially by ANR project IDEALG (ANR-10-BTBR-04) "Investissements d'Avenir, Biotechnologies-Bioressources" and CNRS momentum call (2017). BBD was funded by a joint Ph.D. scholarship from the Brittany region (Project HOSALA) and the Sorbonne University (ED227).	Adams RP., 2007, IDENTIFICATION ESSEN; Amachi S, 2007, APPL ENVIRON MICROB, V73, P7536, DOI 10.1128/AEM.01592-07; Amrouni S., 2014, Phytotherapie (Paris), V12, P309, DOI 10.1007/s10298-014-0842-x; Bakkali F, 2008, FOOD CHEM TOXICOL, V46, P446, DOI 10.1016/j.fct.2007.09.106; Barbeyron T, 2016, ENVIRON MICROBIOL, V18, P4610, DOI 10.1111/1462-2920.13584; Bassole IHN, 2010, MOLECULES, V15, P7825, DOI 10.3390/molecules15117825; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; BERKELMAN RL, 1982, J CLIN MICROBIOL, V15, P635, DOI 10.1128/JCM.15.4.635-639.1982; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Burgunter-Delamare B, 2020, FRONT MAR SCI, V7, DOI 10.3389/fmars.2020.00085; Burt S, 2004, INT J FOOD MICROBIOL, V94, P223, DOI 10.1016/j.ijfoodmicro.2004.03.022; Burt SA, 2003, LETT APPL MICROBIOL, V36, P162, DOI 10.1046/j.1472-765X.2003.01285.x; Carson CF, 2006, CLIN MICROBIOL REV, V19, P50, DOI 10.1128/CMR.19.1.50-62.2006; Carson CF, 2002, ANTIMICROB AGENTS CH, V46, P1914, DOI 10.1128/AAC.46.6.1914-1920.2002; Chamberland M, 1887, ANN I PASTEUR, V1, P153; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Da Silva F, 2010, THESIS; de Billerbeck V. -G., 2007, Phytotherapie (Paris), V5, P249, DOI 10.1007/s10298-007-0265-z; DEANS SG, 1987, INT J FOOD MICROBIOL, V5, P165, DOI 10.1016/0168-1605(87)90034-1; Dittami SM, 2016, ISME J, V10, P51, DOI 10.1038/ismej.2015.104; Dittami SM, 2020, COMMUNITY PERSPECTIV, DOI [10.5281/ZENODO.3696771, DOI 10.5281/ZEN0D0.3696771]; Dorman HJD, 2000, J APPL MICROBIOL, V88, P308, DOI 10.1046/j.1365-2672.2000.00969.x; Eftekhar F, 2005, Z NATURFORSCH C, V60, P821; Fair RJ, 2014, PERSPECT MED CHEM, V6, P25, DOI 10.4137/PMC.S14459; Ferreira JVN, 2016, LANGMUIR, V32, P3234, DOI 10.1021/acs.langmuir.6b00600; Fournier JB, 2014, APPL ENVIRON MICROB, V80, P7561, DOI 10.1128/AEM.02430-14; Gilles M, 2010, FOOD CHEM, V119, P731, DOI 10.1016/j.foodchem.2009.07.021; Goecke F, 2010, MAR ECOL PROG SER, V409, P267, DOI 10.3354/meps08607; Habbadi K, 2018, ENVIRON SCI POLLUT R, V25, P29943, DOI 10.1007/s11356-017-1008-9; Herman A, 2016, CURR MICROBIOL, V72, P165, DOI 10.1007/s00284-015-0933-4; Hollants J, 2013, FEMS MICROBIOL ECOL, V83, P1, DOI 10.1111/j.1574-6941.2012.01446.x; HOUANG ET, 1976, J CLIN PATHOL, V29, P752, DOI 10.1136/jcp.29.8.752; Illumina Inc, 2013, PREP 16S RIB RNA GEN, P1; Kaloustian J., 2008, Phytotherapie (Paris), V6, P160, DOI 10.1007/s10298-008-0307-1; Kanagalingam J, 2015, INT J CLIN PRACT, V69, P1247, DOI 10.1111/ijcp.12707; Kerrison PD, 2016, J APPL PHYCOL, V28, P3423, DOI 10.1007/s10811-016-0873-9; Kientz B, 2011, BOT MAR, V54, P457, DOI 10.1515/BOT.2011.053; KleinJan H, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.02456; Kozich JJ, 2013, APPL ENVIRON MICROB, V79, P5112, DOI 10.1128/AEM.01043-13; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; La Barre S, 2010, MAR DRUGS, V8, P988, DOI 10.3390/md8040988; Lachapelle JM, 2013, CLIN PRACT, V10, P579, DOI DOI 10.2217/CPR.13.50; Lambert RJW, 2001, J APPL MICROBIOL, V91, P453, DOI 10.1046/j.1365-2672.2001.01428.x; LAWLOR HJ, 1991, BOT MAR, V34, P261, DOI 10.1515/botm.1991.34.3.261; Leblanc C, 2006, BIOCHIMIE, V88, P1773, DOI 10.1016/j.biochi.2006.09.001; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; Marchese A, 2017, MATERIALS, V10, DOI 10.3390/ma10080947; Marchese A, 2016, FOOD CHEM, V210, P402, DOI 10.1016/j.foodchem.2016.04.111; Margulis L., 1991, SYMBIOSIS SOURCE EVO, P1; Masella AP, 2012, BMC BIOINFORMATICS, V13, DOI 10.1186/1471-2105-13-31; McCulloch EC, 1936, STERILIZATION DISINF; McDonnell G, 1999, CLIN MICROBIOL REV, V12, P147, DOI 10.1128/CMR.12.1.147; Mora FD, 2011, NAT PROD COMMUN, V6, P1051; Mousavi SM, 2011, AQUACULT INT, V19, P205, DOI 10.1007/s10499-010-9354-3; Nelson RRS, 1997, J ANTIMICROB CHEMOTH, V40, P305, DOI 10.1093/jac/40.2.305; Ponce AG, 2003, LEBENSM-WISS TECHNOL, V36, P679, DOI 10.1016/S0023-6438(03)00088-4; Provasoli L, 1974, ALGAL PHYSL BIOCH, P741; RACKUR H, 1985, J HOSP INFECT, V6, P13, DOI 10.1016/S0195-6701(85)80041-4; Suvega T, 2019, BIOCATAL AGR BIOTECH, V19, DOI 10.1016/j.bcab.2019.101136; Tapia JE, 2016, FRONT MICROBIOL, V7, DOI [10.3389/fmicb.2016.00197, 10.3389/fmicb.2016.00107]; Thomas F, 2019, ENV MICROBIOL REP, V1758-2229, P12806; Ultee A, 1999, APPL ENVIRON MICROB, V65, P4606; Underwood A.J., 1981, Oceanography and Marine Biology an Annual Review, V19, P513; Wahl M, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00292; Watts JEM, 2017, MAR DRUGS, V15, DOI 10.3390/md15060158; ZAMORA JL, 1986, AM J SURG, V151, P400, DOI 10.1016/0002-9610(86)90477-0	66	0	0	4	4	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0921-8971	1573-5176		J APPL PHYCOL	J. Appl. Phycol.	FEB	2021	33	1			SI		459	470		10.1007/s10811-020-02286-y		NOV 2020	12	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	QB5AA	WOS:000586341500002					2021-04-07	
J	Rathor, P; Borza, T; Liu, YH; Qin, Y; Stone, S; Zhang, JZ; Hui, JPM; Berrue, F; Groisillier, A; Tonon, T; Yurgel, S; Potin, P; Prithiviraj, B				Rathor, Pramod; Borza, Tudor; Liu, Yanhui; Qin, Yuan; Stone, Sophia; Zhang, Junzeng; Hui, Joseph P. M.; Berrue, Fabrice; Groisillier, Agnes; Tonon, Thierry; Yurgel, Svetlana; Potin, Philippe; Prithiviraj, Balakrishnan			Low Mannitol Concentrations in Arabidopsis thaliana Expressing Ectocarpus Genes Improve Salt Tolerance	PLANTS-BASEL			English	Article						mannitol biosynthesis genes; mannitol-1-phosphate dehydrogenase; mannitol-1-phosphatase; Ectocarpus sp; Arabidopsis thaliana; abiotic stress tolerance; salt stress	ABSCISIC-ACID; MANNITOL-1-PHOSPHATE DEHYDROGENASE; TRANSCRIPTION FACTOR; TRANSGENIC TOBACCO; STRESS RESPONSES; OSMOTIC-STRESS; WATER-STRESS; OVEREXPRESSION; DROUGHT; CHANNEL	Mannitol is abundant in a wide range of organisms, playing important roles in biotic and abiotic stress responses. Nonetheless, mannitol is not produced by a vast majority of plants, including many important crop plants. Mannitol-producing transgenic plants displayed improved tolerance to salt stresses though mannitol production was rather low, in the mu M range, compared to mM range found in plants that innately produce mannitol. Little is known about the molecular mechanisms underlying salt tolerance triggered by low concentrations of mannitol. Reported here is the production of mannitol in Arabidopsis thaliana, by expressing two mannitol biosynthesis genes from the brown alga Ectocarpus sp. strain Ec32. To date, no brown algal genes have been successfully expressed in land plants. Expression of mannitol-1-phosphate dehydrogenase and mannitol-1-phosphatase genes was associated with the production of 42.3-52.7 nmol g(-1) fresh weight of mannitol, which was sufficient to impart salinity and temperature stress tolerance. Transcriptomics revealed significant differences in the expression of numerous genes, in standard and salinity stress conditions, including genes involved in K+ homeostasis, ROS signaling, plant development, photosynthesis, ABA signaling and secondary metabolism. These results suggest that the improved tolerance to salinity stress observed in transgenic plants producing mannitol in mu M range is achieved by the activation of a significant number of genes, many of which are involved in priming and modulating the expression of genes involved in a variety of functions including hormone signaling, osmotic and oxidative stress, and ion homeostasis.	[Rathor, Pramod; Borza, Tudor; Yurgel, Svetlana; Prithiviraj, Balakrishnan] Dalhousie Univ, Dept Plant Food & Environm Sci, Marine Bioprod Res Lab, Truro, NS B2N 5E3, Canada; [Liu, Yanhui; Qin, Yuan] Fujian Agr & Forestry Univ, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Ctr Genom & Biotechnol, Coll Life Sci,State Key Lab Ecol Pest Control Fuj, Fuzhou 350002, Peoples R China; [Stone, Sophia] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4R2, Canada; [Zhang, Junzeng; Hui, Joseph P. M.; Berrue, Fabrice] Natl Res Council Canada, Aquat & Crop Resource Dev Res Ctr, Halifax, NS B3H 3Z1, Canada; [Groisillier, Agnes] Univ Nantes, Unite Fonct & Ingn Prot UFIP, CNRS, UMR 6286, F-44322 Nantes, France; [Tonon, Thierry] Univ York, Dept Biol, Ctr Novel Agr Prod, Heslington YO10 5DD, England; [Tonon, Thierry; Potin, Philippe] Sorbonne Univ, CNRS, UMR 8227, Stn Biol,Integrat Biol Marine Models LBI2M, F-29680 Roscoff, France	Prithiviraj, B (corresponding author), Dalhousie Univ, Dept Plant Food & Environm Sci, Marine Bioprod Res Lab, Truro, NS B2N 5E3, Canada.	pramod.rathor@dal.ca; tudor.borza@dal.ca; 2180514010@fafu.edu.cn; yuanqin@fafu.edu.cn; s.stone@dal.ca; junzeng.zhang@nrc-cnrc.gc.ca; Joseph.Hui@nrc-cnrc.gc.ca; Fabrice.Berrue@nrc-cnrc.gc.ca; agnes.groisillier@univ-nantes.fr; thierry.tonon@york.ac.uk; syurgel@dal.ca; philippe.potin@sb-roscoff.fr; bprithiviraj@dal.ca	Borza, Tudor/M-4937-2019; Borza, Tudor/A-8510-2012; Tonon, Thierry/A-3214-2009	Borza, Tudor/0000-0002-2527-6551; Borza, Tudor/0000-0002-2527-6551; Berrue, Fabrice/0000-0003-2389-4388; Zhang, Junzeng/0000-0003-0118-0317; Tonon, Thierry/0000-0002-1454-6018; qin, yuan/0000-0003-4713-6151	NSERC-DG grant [1177546]; MITACS; France-Canada Research Fund (FCRF); NSERC Industrial Postgraduate ScholarshipNatural Sciences and Engineering Research Council of Canada (NSERC); MITACS Globalink Research Award; Agence Nationale de la Recherche via the investment expenditure program IDEALG GrantFrench National Research Agency (ANR) [ANR-10-BTBR-04]	B.P.'s lab was supported by NSERC-DG grant 1177546, MITACS and France-Canada Research Fund (FCRF). P.R. was supported by an NSERC Industrial Postgraduate Scholarship and a MITACS Globalink Research Award. P.P. and T.T. were also supported by the Agence Nationale de la Recherche via the investment expenditure program IDEALG Grant ANR-10-BTBR-04.	Abebe T, 2003, PLANT PHYSIOL, V131, P1748, DOI 10.1104/pp.102.003616; Akhter S, 2016, PLANT BIOTECHNOL J, V14, P215, DOI 10.1111/pbi.12376; Antoni R, 2012, PLANT PHYSIOL, V158, P970, DOI 10.1104/pp.111.188623; Apse MP, 2003, PLANT J, V36, P229, DOI 10.1046/j.1365-313X.2003.01871.x; Babicki S, 2016, NUCLEIC ACIDS RES, V44, pW147, DOI 10.1093/nar/gkw419; BLACK WAP, 1950, J MAR BIOL ASSOC UK, V29, P45, DOI 10.1017/S0025315400056186; Bonin P, 2015, PHYTOCHEMISTRY, V117, P509, DOI 10.1016/j.phytochem.2015.07.015; Chan ZL, 2011, J EXP BOT, V62, P4787, DOI 10.1093/jxb/err130; Chang CCC, 2009, PLANT PHYSIOL, V150, P670, DOI 10.1104/pp.109.135566; Chiang YJ, 2005, J AM SOC HORTIC SCI, V130, P605, DOI 10.21273/JASHS.130.4.605; Clough SJ, 1998, PLANT J, V16, P735, DOI 10.1046/j.1365-313x.1998.00343.x; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Demidchik V, 2010, J CELL SCI, V123, P1468, DOI 10.1242/jcs.064352; Dixon DP, 1998, CURR OPIN PLANT BIOL, V1, P258, DOI 10.1016/S1369-5266(98)80114-3; Fujii H, 2009, NATURE, V462, P660, DOI 10.1038/nature08599; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Groisillier A, 2014, J EXP BOT, V65, P559, DOI 10.1093/jxb/ert405; Guo KM, 2008, PHYSIOL PLANTARUM, V134, P499, DOI 10.1111/j.1399-3054.2008.01157.x; Heberle H, 2015, BMC BIOINFORMATICS, V16, DOI 10.1186/s12859-015-0611-3; Hundertmark M, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-118; Jia FJ, 2014, BIOCHEM BIOPH RES CO, V454, P505, DOI 10.1016/j.bbrc.2014.10.136; Kempa S, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003935; Kiyosue T, 1998, BBA-BIOMEMBRANES, V1370, P187, DOI 10.1016/S0005-2736(98)00007-8; Laohavisit A, 2013, PLANT PHYSIOL, V163, P253, DOI 10.1104/pp.113.217810; Lee S, 2004, PLANT CELL, V16, P1378, DOI 10.1105/tpc.021683; Li L, 2015, P NATL ACAD SCI USA, V112, P14734, DOI 10.1073/pnas.1514670112; Lloyd JR, 2005, TRENDS PLANT SCI, V10, P130, DOI 10.1016/j.tplants.2005.01.001; Mittler R, 2002, TRENDS PLANT SCI, V7, P405, DOI 10.1016/S1360-1385(02)02312-9; Montecinos AE, 2017, J PHYCOL, V53, P17, DOI 10.1111/jpy.12452; Osakabe Y, 2013, PLANT CELL, V25, P609, DOI 10.1105/tpc.112.105700; Patel T. K., 2015, American Journal of Plant Sciences, V6, P1116, DOI 10.4236/ajps.2015.68116; Patel TK, 2016, TRENDS PLANT SCI, V21, P486, DOI 10.1016/j.tplants.2016.01.006; Pilot G, 2003, PLANT MOL BIOL, V51, P773, DOI 10.1023/A:1022597102282; Prabhavathi V, 2007, PLANT SCI, V173, P50, DOI 10.1016/j.plantsci.2007.04.004; Qi MS, 2019, PLANT BIOTECHNOL J, V17, P252, DOI 10.1111/pbi.12961; Qin XQ, 2002, PLANT PHYSIOL, V128, P544, DOI 10.1104/pp.010663; Qiu QS, 2003, PLANT PHYSIOL, V132, P1041, DOI 10.1104/pp.102.010421; Rook F, 2006, PLANT CELL ENVIRON, V29, P426, DOI 10.1111/j.1365-3040.2005.01477.x; Rossel JB, 2006, PLANT CELL ENVIRON, V29, P269, DOI 10.1111/j.1365-3040.2005.01419.x; Sakuma Y, 2006, P NATL ACAD SCI USA, V103, P18822, DOI 10.1073/pnas.0605639103; Sickler CM, 2007, FUNCT PLANT BIOL, V34, P382, DOI 10.1071/FP06274; Soon FF, 2012, SCIENCE, V335, P85, DOI 10.1126/science.1215106; TARCZYNSKI MC, 1992, P NATL ACAD SCI USA, V89, P2600, DOI 10.1073/pnas.89.7.2600; TARCZYNSKI MC, 1993, SCIENCE, V259, P508, DOI 10.1126/science.259.5094.508; Thalmann M, 2017, NEW PHYTOL, V214, P943, DOI 10.1111/nph.14491; THOMAS JC, 1995, PLANT CELL ENVIRON, V18, P801, DOI 10.1111/j.1365-3040.1995.tb00584.x; Tonon T, 2017, NEW PHYTOL, V213, P1573, DOI 10.1111/nph.14358; Tunnacliffe A, 2007, NATURWISSENSCHAFTEN, V94, P791, DOI 10.1007/s00114-007-0254-y; Verslues PE, 2006, PLANT J, V45, P523, DOI 10.1111/j.1365-313X.2005.02593.x; Wang WX, 2004, TRENDS PLANT SCI, V9, P244, DOI 10.1016/j.tplants.2004.03.006; Willekens H, 1997, EMBO J, V16, P4806, DOI 10.1093/emboj/16.16.4806; Yoo JH, 2005, J BIOL CHEM, V280, P3697, DOI 10.1074/jbc.M408237200; Zhang HX, 2001, NAT BIOTECHNOL, V19, P765, DOI 10.1038/90824; Zhifang G, 2003, PLANT CELL ENVIRON, V26, P275, DOI 10.1046/j.1365-3040.2003.00958.x; Zhu JK, 2016, CELL, V167, P313, DOI 10.1016/j.cell.2016.08.029	55	1	1	3	3	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		2223-7747		PLANTS-BASEL	Plants-Basel	NOV	2020	9	11							1508	10.3390/plants9111508			18	Plant Sciences	Plant Sciences	OX7DJ	WOS:000593720400001	33171775	DOAJ Gold, Green Accepted, Green Published			2021-04-07	
J	Celis-Pla, PSM; Moenne, F; Rodriguez-Rojas, F; Pardo, D; Lavergne, C; Moenne, A; Brown, MT; Huovinen, P; Gomez, I; Navarro, N; Saez, CA				Celis-Pla, Paula S. M.; Moenne, Fabiola; Rodriguez-Rojas, Fernanda; Pardo, Diego; Lavergne, Celine; Moenne, Alejandra; Brown, Murray T.; Huovinen, Pirjo; Gomez, Ivan; Navarro, Nelso; Saez, Claudio A.			Antarctic intertidal macroalgae under predicted increased temperatures mediated by global climate change: Would they cope?	SCIENCE OF THE TOTAL ENVIRONMENT			English	Article						Rhodophyceae; Ulvophyceae; Phaeophyceae; Global warming; Extremophiles	ECTOCARPUS-SILICULOSUS; GENE-EXPRESSION; ANTIOXIDANT RESPONSES; H2O2 PRODUCTION; UV-RADIATION; LIGHT; BROWN; TOLERANCE; PHOTOINHIBITION; PHOTOSYNTHESIS	The Antarctic Peninsula is one of the regions to be most affected by increase in sea surface temperatures (SSTs) mediated by Global Climate Change; indeed, most negative predictions imply an up to 6 degrees C increment by the end of the XXI century. Temperature is one of the most important factors mediating diversity and distribution of macroalgae, although there is still no consensus as to the likely effects of higher SSTs, especially for polar seaweeds. Some available information suggests that potential strategies to withstand future increases in SSTs will be founded upon the glutathione-ascorbate cycle and the induction of chaperone-functioning heat shock proteins (HSPs); however, their eventual role, even for general stress responses, is unclear. The intertidal green, brown and red macroalgae species Monostroma hariotii, Adenocystis utricularis and Pyropia endiviifolia, respectively, from King George Island, Antarctic Peninsula, were exposed to 2 degrees C (control) and 8 degrees C (climate change scenario) for up to 5 days (d). Photosynthetic activity (alpha(ETR) and ETRmax and Ek(ETR)), photoinhibition (F-v/F-m) and photoprotection processes (alpha(NPQ), NPQ(max) and Ek(NPQ)) provided no evidence of negative ecophysiological effects. There were moderate increases in H2O2 production and levels of lipid peroxidation with temperature, results supported by stable levels of total glutathione and ascorbate pools, with mostly higher levels of reduced ascorbate and glutathione than oxidized forms in all species. Transcripts of P. endivdfolia indicated a general upregulation of all antioxidant enzymes and HSPs genes studied under warmer temperature, although with different levels of activation with time. This pioneering investigation exploring different levels of biological organization, suggested that Antarctic intertidal macroalgae may be able to withstand future rise in SSTs, probably slightly altering their latitudinal distribution and/or range of thermal tolerance, by exhibiting robust glutathione-ascorbate production and recycling, as well as the induction of associated antioxidant enzymatic machinery and the syntheses of HSPs. (C) 2020 Elsevier B.V. All rights reserved.	[Celis-Pla, Paula S. M.; Moenne, Fabiola; Rodriguez-Rojas, Fernanda; Pardo, Diego; Lavergne, Celine; Saez, Claudio A.] Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar, Chile; [Celis-Pla, Paula S. M.; Moenne, Fabiola; Rodriguez-Rojas, Fernanda; Pardo, Diego; Lavergne, Celine; Saez, Claudio A.] Univ Playa Ancha, HUB Ambiental UPLA, Valparaiso, Chile; [Pardo, Diego] Univ Playa Ancha, Fac Ingn, Dept Medio Ambiente, Valparaiso, Chile; [Lavergne, Celine] Pontificia Univ Catolica Valparaiso, Escuela Ingn Bioquim, Ave Brasil 2085, Valparaiso 2340950, Chile; [Moenne, Alejandra] Fac Quim & Biol, Dept Biol, Lab Marine Biotechnol, Estn Cent, Valparaiso, Chile; [Brown, Murray T.] Univ Plymouth, Sch Biol & Marine Sci, Plymouth, Devon, England; [Huovinen, Pirjo; Gomez, Ivan] Univ Austral Chile, Fac Ciencias, Inst Ciencias Marinas & Limnol, Valdivia, Chile; [Huovinen, Pirjo; Gomez, Ivan; Navarro, Nelso] Ctr Invest Dinam Ecosistemas Marinos Altas Latitu, Punta Arenas, Chile; [Navarro, Nelso] Univ Magallanes, Fac Ciencias, Dept Ciencias & Recursos Nat, Punta Arenas, Chile	Saez, CA (corresponding author), Univ Playa Ancha, HUB Ambiental UPLA, Valparaiso, Chile.	claudio.saez@upla.cl	Lavergne, Celine/B-4208-2015; Saez, Claudio/F-5978-2015	Lavergne, Celine/0000-0002-2002-8655; Saez, Claudio/0000-0002-5037-3484; Moenne, Alejandra/0000-0002-7309-9713	projects INACH (Chilean Antarctic Institute) [RT_09_16, RG_10_18]; Fondap IDEAL [15150003]; ANID FONDECYT Postdoctorado [3180374, 3180394]	This work was financed and logistic support granted through the projects INACH (Chilean Antarctic Institute) RT_09_16 and RG_10_18 directed by C.A. Saez and P. S. M. Celis-Pla, respectively. P. Huovinen and I. Gomez were funded by Fondap IDEAL 15150003, while C. Lavergne was funded by ANID FONDECYT Postdoctorado (#3180374) and Fernanda Rodriguez-Rojas by ANID FONDECYT Postdoctorado (#3180394).	Aral MM, 2016, WATER-SUI, V8, DOI 10.3390/w8110519; Aranda PS, 2012, ELECTROPHORESIS, V33, P366, DOI 10.1002/elps.201100335; Barati B, 2019, ACTA PHYSIOL PLANT, V41, DOI 10.1007/s11738-019-2813-1; Becker S, 2011, ANTARCT SCI, V23, P419, DOI 10.1017/S0954102011000575; Cardenas CA, 2018, PEERJ, V6, DOI 10.7717/peerj.4289; Celis-Pla PSM, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20184547; Celis-Pla PSM, 2018, MAR POLLUT BULL, V128, P214, DOI 10.1016/j.marpolbul.2018.01.005; Celis-Pla PSM, 2016, MAR ENVIRON RES, V115, P89, DOI 10.1016/j.marenvres.2015.11.014; Cruces E, 2013, MAR BIOL, V160, P1, DOI 10.1007/s00227-012-2049-8; Cruces E, 2012, PHOTOCHEM PHOTOBIOL, V88, P58, DOI 10.1111/j.1751-1097.2011.01013.x; Di Bari P, 2019, J HIGH ENERGY PHYS, DOI 10.1007/JHEP05(2019)011; EILERS PHC, 1988, ECOL MODEL, V42, P199, DOI 10.1016/0304-3800(88)90057-9; Fan MH, 2018, J APPL PHYCOL, V30, P3361, DOI 10.1007/s10811-018-1567-2; Fan MH, 2018, PROTEOME SCI, V16, DOI 10.1186/s12953-018-0145-5; Figueroa FL, 2014, AQUAT BIOL, V22, P195, DOI 10.3354/ab00593; Figueroa FL, 2019, ALGAL RES, V41, DOI 10.1016/j.algal.2019.101560; Figueroa FL, 2014, MAR ENVIRON RES, V97, P30, DOI 10.1016/j.marenvres.2014.01.009; Figueroa FL, 2009, AQUAT BIOL, V7, P159, DOI 10.3354/ab00186; Figueroa FL, 2003, PHOTOSYNTH RES, V75, P259, DOI 10.1023/A:1023936313544; Foyer CH, 2011, PLANT PHYSIOL, V155, P2, DOI 10.1104/pp.110.167569; Gomez F, 2008, POSTHARVEST BIOL TEC, V49, P229, DOI 10.1016/j.postharvbio.2008.02.012; Gomez I., 2020, ANTARCTIC SEAWEEDS D, P397; Gomez I, 2009, BOT MAR, V52, P593, DOI 10.1515/BOT.2009.073; Grzymski J, 1997, J PHYCOL, V33, P408, DOI 10.1111/j.0022-3646.1997.00408.x; Hammann M, 2016, MAR BIOL, V163, DOI 10.1007/s00227-016-2881-3; HANELT D, 1995, BOT ACTA, V108, P99, DOI 10.1111/j.1438-8677.1995.tb00838.x; Heinrich S, 2015, J PHYCOL, V51, P93, DOI 10.1111/jpy.12255; Iniguez C, 2017, J EXP BOT, V68, P3971, DOI 10.1093/jxb/erx164; Jacob P, 2017, PLANT BIOTECHNOL J, V15, P405, DOI 10.1111/pbi.12659; Jueterbock A, 2016, ECOL EVOL, V6, P1712, DOI 10.1002/ece3.2001; Kim K, 2004, FEBS LETT, V571, P124, DOI 10.1016/j.febslet.2004.06.064; Kremb S, 2012, PHYCOL RES, V60, P151, DOI 10.1111/j.1440-1835.2012.00647.x; Kumar M, 2014, ADV BOT RES, V71, P91, DOI 10.1016/B978-0-12-408062-1.00004-4; Lee R. E., 2008, PHYCOLOGY; Li HR, 2020, PHYSIOL PLANTARUM, V168, P5, DOI 10.1111/ppl.13009; Liu L, 2018, J APPL PHYCOL, V30, P1271, DOI 10.1007/s10811-017-1316-y; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Lu N, 2013, J APPL PHYCOL, V25, P1925, DOI 10.1007/s10811-013-0020-9; Maharana D, 2015, ENVIRON SCI POLLUT R, V22, P18741, DOI 10.1007/s11356-015-4985-6; Distefano AM, 2017, J CELL BIOL, V216, P463, DOI 10.1083/jcb.201605110; Mellard JP, 2015, P ROY SOC B-BIOL SCI, V282, DOI 10.1098/rspb.2014.1351; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Muller R, 2012, PHYCOL RES, V60, P27, DOI 10.1111/j.1440-1835.2011.00630.x; Mulvaney R, 2012, NATURE, V489, P141, DOI 10.1038/nature11391; Navarrete A, 2019, PLANT PHYSIOL BIOCH, V135, P423, DOI 10.1016/j.plaphy.2018.11.019; Navarro NP, 2016, REV CHIL HIST NAT, V89, DOI 10.1186/s40693-016-0051-0; Ortega KJ, 2014, REV BIOL MAR OCEANOG, V49, P129, DOI 10.4067/S0718-19572014000100014; Rautenberger R, 2006, POLAR BIOL, V29, P988, DOI 10.1007/s00300-006-0141-6; Rautenberger R, 2015, MAR BIOL, V162, P1087, DOI 10.1007/s00227-015-2651-7; Rodriguez-Rojas F, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20184546; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Flores-Molina MR, 2016, PHOTOCHEM PHOTOBIOL, V92, P455, DOI 10.1111/php.12580; Saez CA, 2015, PHYCOLOGIA, V54, P425, DOI 10.2216/15-30.1; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; Saroussi S, 2007, AQUAT BOT, V86, P89, DOI 10.1016/j.aquabot.2006.09.003; Schreiber U., 1995, ECOPHYSIOLOGY PHOTOS, P49, DOI [DOI 10.1007/978-3-642-79354-7_3, 10.1007/978-3-642-79354-7_3]; Smirnoff N, 2019, NEW PHYTOL, V221, P1197, DOI 10.1111/nph.15488; Smolina I, 2016, ROY SOC OPEN SCI, V3, DOI 10.1098/rsos.150429; Solomon S., 2007, CLIMATE CHANGE 2007; Tuckett PA, 2019, NAT COMMUN, V10, DOI 10.1038/s41467-019-12039-2; Underwood A.J., 1996, EXPT ECOLOGY THEIR L; Valdivia N, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0100714; Wang WL, 2018, PLOS ONE, V13, DOI 10.1371/journal.pone.0195842; Ware MA, 2015, PHOTOSYNTH RES, V126, P261, DOI 10.1007/s11120-015-0102-4; Wiencke C, 2007, LIFE EXTREME ENV, P213; Wiencke C., 2011, BIOL POLAR BENTHIC A; Zacher K, 2009, BOT MAR, V52, P483, DOI 10.1515/BOT.2009.082	69	0	0	52	71	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0048-9697	1879-1026		SCI TOTAL ENVIRON	Sci. Total Environ.	OCT 20	2020	740								140379	10.1016/j.scitotenv.2020.140379			12	Environmental Sciences	Environmental Sciences & Ecology	NE1TF	WOS:000562379400015	32927555				2021-04-07	
J	Rodriguez-Rojas, F; Lopez-Marras, A; Celis-Pla, PSM; Munoz, P; Garcia-Bartolomei, E; Valenzuela, F; Orrego, R; Carratala, A; Sanchez-Lizaso, JL; Saez, CA				Rodriguez-Rojas, Fernanda; Lopez-Marras, Americo; Celis-Pla, Paula S. M.; Munoz, Pamela; Garcia-Bartolomei, Enzo; Valenzuela, Fernando; Orrego, Rodrigo; Carratala, Adoracion; Luis Sanchez-Lizaso, Jose; Saez, Claudio A.			Ecophysiological and cellular stress responses in the cosmopolitan brown macroalga Ectocarpus as biomonitoring tools for assessing desalination brine impacts	DESALINATION			English	Article						Brine; Desalination; Oxidative stress; Macroalgae	SEAGRASS POSIDONIA-OCEANICA; ANTIOXIDANT DEFENSE SYSTEM; SILICULOSUS ECTOCARPALES; CYMODOCEA-NODOSA; SALINITY STRESS; GENE-EXPRESSION; SEAWATER; GROWTH; MODEL; PHOTOSYNTHESIS	Seawater desalination via reverse osmosis (SWRO) is highlighted as one of the most feasible solutions for obtaining freshwater. However, brine produced by SWRO is generally discharged to the subtidal area potentially causing detrimental effects on benthic organisms. In this study, we evaluated for the first time, ecophysiological and cellular responses of brown macroalgae as diagnosis tools to assess environmental impacts of desalination, through transplantation experiments with the cosmopolitan brown alga Ectocarpus. Transplants located at 10 and 30 m from the discharge point of a desalination plant located in Antofagasta, Chile, showed impaired photosynthetic parameters (ETR, Fv/Fm, alpha(ETR) and ETRmax) and oxidative stress responses like accumulation of H2O2 and enhanced lipid peroxidation. Also, increased salinity produced high accumulation of ascorbate but a decrease in glutathione content. Also, genes encoding for enzymes related to salinity tolerance, SOS2, and oxidative stress, SOD, APX, PRX and GR, were highly up-regulated in transplanted Ectocarpus, especially at 10 m from the brine discharge. Altogether, our results demonstrate that Ectocarpus is a sensitive species to brine impacts, and that the transplantation method combined with its physiological and molecular responses are reliable tools to incorporate in environmental monitoring plans to address for desalination brine impacts on coastal ecosystems.	[Rodriguez-Rojas, Fernanda; Lopez-Marras, Americo; Celis-Pla, Paula S. M.; Munoz, Pamela; Saez, Claudio A.] Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar, Chile; [Rodriguez-Rojas, Fernanda; Lopez-Marras, Americo; Celis-Pla, Paula S. M.; Munoz, Pamela; Saez, Claudio A.] Univ Playa Ancha, HUB AMBIENTAL UPLA, Valparaiso, Chile; [Munoz, Pamela] Univ Playa Ancha, Fac Ciencias Nat & Exactas, Doctorado Interdisciplinario Ciencias Ambientales, Valparaiso, Chile; [Munoz, Pamela] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, Doctorado Ciencias Mar & Biol Aplicada, Alicante, Spain; [Garcia-Bartolomei, Enzo] Univ Concepcion, Ctr Recursos Hidr Agr & Min, Concepcion, Chile; [Garcia-Bartolomei, Enzo] Univ Concepcion, Ctr EULA, Doctorado Ciencias Ambientales, Concepcion, Chile; [Valenzuela, Fernando] Univ Antofagasta, Ctr Bioinnovac, Antofagasta, Chile; [Orrego, Rodrigo] Univ Antofagasta, Inst Ciencias Nat Alexander Humboldt, Lab Toxicol Acuat AQUATOX, Antofagasta, Chile; [Carratala, Adoracion] Univ Alicante, Dept Ingn Quim, Alicante, Spain; [Luis Sanchez-Lizaso, Jose] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, Alicante, Spain	Rodriguez-Rojas, F; Saez, CA (corresponding author), Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar, Chile.; Rodriguez-Rojas, F; Saez, CA (corresponding author), Univ Playa Ancha, HUB AMBIENTAL UPLA, Valparaiso, Chile.	fernanda.rodriguez@upla.cl; claudio.saez@upla.cl	Lizaso, Jose Luis L Sanchez/J-4939-2017; Saez, Claudio/F-5978-2015; Orrego, Rodrigo/U-9077-2018	Lizaso, Jose Luis L Sanchez/0000-0002-3927-5699; Saez, Claudio/0000-0002-5037-3484; Orrego, Rodrigo/0000-0002-7606-2828; Carratala, Adoracion/0000-0002-8531-1814	postdoctoral fellowship - Fondecyt program (CONICYT), Chile [3180394]; CONICYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) [21171486]; IDA Channabasappa Memorial Scholarship	This work was funded by the postdoctoral fellowship #3180394 granted by Fondecyt program (CONICYT), Chile, to FRR. EGB was funded by CONICYT doctoral scholarship #21171486 and a IDA Channabasappa Memorial Scholarship.	[Anonymous], 2015, 4 UN WORLD WAT DEV, V1; Aranda PS, 2012, ELECTROPHORESIS, V33, P366, DOI 10.1002/elps.201100335; Bellgrove A, 2017, MAR POLLUT BULL, V117, P17, DOI 10.1016/j.marpolbul.2017.02.012; Bose J, 2014, J EXP BOT, V65, P1241, DOI 10.1093/jxb/ert430; Cambridge ML, 2019, MAR POLLUT BULL, V140, P462, DOI 10.1016/j.marpolbul.2019.02.001; Celis-Pla PSM, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20184547; Celis-Pla PSM, 2018, MAR POLLUT BULL, V128, P214, DOI 10.1016/j.marpolbul.2018.01.005; Celis-Pla PSM, 2016, MAR ENVIRON RES, V115, P89, DOI 10.1016/j.marenvres.2015.11.014; Celis-Pla PSM, 2014, SCI MAR, V78, P377, DOI 10.3989/scimar.04053.05A; Clark GF, 2018, WATER RES, V145, P757, DOI 10.1016/j.watres.2018.08.071; Del-Pilar-Ruso Y, 2015, WATER RES, V70, P325, DOI 10.1016/j.watres.2014.11.036; Dernetriou G, 2007, BBA-BIOENERGETICS, V1767, P272, DOI 10.1016/j.bbabio.2007.02.020; EILERS PHC, 1988, ECOL MODEL, V42, P199, DOI 10.1016/0304-3800(88)90057-9; Elazzaoui Ahmed, 2019, ScientificWorldJournal, V2019, P5875027, DOI 10.1155/2019/5875027; Fernandez-Torquemada Y, 2005, J EXP MAR BIOL ECOL, V320, P57, DOI 10.1016/j.jembe.2004.12.019; Fernandez-Torquemada Y, 2013, DESALIN WATER TREAT, V51, P567, DOI 10.1080/19443994.2012.716609; Figueroa FL, 2014, MAR ENVIRON RES, V97, P30, DOI 10.1016/j.marenvres.2014.01.009; Foyer CH, 2011, PLANT PHYSIOL, V155, P2, DOI 10.1104/pp.110.167569; Frank H, 2019, WATER RES, V151, P478, DOI 10.1016/j.watres.2018.12.046; Gao L, 2017, WATER-SUI, V9, DOI 10.3390/w9100763; Garrote-Moreno A, 2014, MAR POLLUT BULL, V81, P61, DOI 10.1016/j.marpolbul.2014.02.019; Garrote-Moreno A, 2016, ESTUAR COAST SHELF S, V181, P209, DOI 10.1016/j.ecss.2016.08.034; Haddeland I, 2014, P NATL ACAD SCI USA, V111, P3251, DOI 10.1073/pnas.1222475110; Hanin M, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.01787; Imlay JA, 2003, ANNU REV MICROBIOL, V57, P395, DOI 10.1146/annurev.micro.57.030502.090938; Ji HT, 2013, MOL PLANT, V6, P275, DOI 10.1093/mp/sst017; Jones E, 2019, SCI TOTAL ENVIRON, V657, P1343, DOI 10.1016/j.scitotenv.2018.12.076; Kim HJ, 2015, INT J MOL SCI, V16, P12261, DOI 10.3390/ijms160612261; Lattemann S., 2003, SEAWATER DESALINATIO; Lee HJ, 2018, J MICROBIOL BIOTECHN, V28, P1217, DOI 10.4014/jmb.1802.02024; Li LH, 2010, WATER RESOUR MANAG, V24, P83, DOI 10.1007/s11269-009-9438-x; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Lu IF, 2006, MAR BIOL, V150, P1, DOI 10.1007/s00227-006-0323-3; Luo MB, 2011, J EXP MAR BIOL ECOL, V409, P223, DOI 10.1016/j.jembe.2011.08.023; Marin-Guirao L, 2013, MAR ENVIRON RES, V84, P60, DOI 10.1016/j.marenvres.2012.12.001; Marin-Guirao L, 2011, ESTUAR COAST SHELF S, V92, P286, DOI 10.1016/j.ecss.2011.01.003; Sandoval-Gil JM, 2012, MAR BIOL, V159, P1129, DOI 10.1007/s00227-012-1892-y; Missimer TM, 2018, DESALINATION, V434, P198, DOI 10.1016/j.desal.2017.07.012; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Petersen KL, 2018, WATER RES, V144, P183, DOI 10.1016/j.watres.2018.07.009; Provasoli L., 1966, P US JAP C HAK SEPT; Roberts DA, 2010, WATER RES, V44, P5117, DOI 10.1016/j.watres.2010.04.036; Saez CA, 2015, PHYCOLOGIA, V54, P425, DOI 10.2216/15-30.1; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Sanchez-Lizaso JL, 2008, DESALINATION, V221, P602, DOI 10.1016/j.desal.2007.01.119; Sandoval-Gil JM, 2014, MAR ENVIRON RES, V95, P39, DOI 10.1016/j.marenvres.2013.12.011; SCHREIBER U, 1995, PLANT CELL PHYSIOL, V36, P873, DOI 10.1093/oxfordjournals.pcp.a078833; Shi HZ, 2002, PLANT CELL, V14, P465, DOI 10.1105/tpc.010371; Singh P, 2019, J PHYCOL, V55, P60, DOI 10.1111/jpy.12777; Sung MS, 2009, MAR BIOTECHNOL, V11, P199, DOI 10.1007/s10126-008-9134-5; Tomaz A, 2020, ENVIRON MONIT ASSESS, V192, DOI 10.1007/s10661-019-8048-1; Touchette BW, 2007, J EXP MAR BIOL ECOL, V350, P194, DOI 10.1016/j.jembe.2007.05.037; Underwood AJ., 1997, EXPT ECOLOGY THEIR L; Yang YQ, 2018, J INTEGR PLANT BIOL, V60, P796, DOI 10.1111/jipb.12689; Yuan HJ, 2011, PLANT CELL REP, V30, P1503, DOI 10.1007/s00299-011-1060-8; Zheng MS, 2019, ENVIRON SCI POLLUT R, V26, P19982, DOI 10.1007/s11356-019-05364-y; Zuo ZJ, 2014, BIOLOGIA, V69, P1314, DOI 10.2478/s11756-014-0437-x	58	1	1	0	10	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0011-9164	1873-4464		DESALINATION	Desalination	SEP 1	2020	489								114527	10.1016/j.desal.2020.114527			9	Engineering, Chemical; Water Resources	Engineering; Water Resources	MC5EE	WOS:000543309100005					2021-04-07	
J	Badstober, J; Gachon, CMM; Ludwig-Muller, J; Sandbichler, AM; Neuhauser, S				Badstoeber, Julia; Gachon, Claire M. M.; Ludwig-Mueller, Jutta; Sandbichler, Adolf M.; Neuhauser, Sigrid			Demystifying biotrophs: FISHing for mRNAs to decipher plant and algal pathogen-host interaction at the single cell level	SCIENTIFIC REPORTS			English	Article							PLASMODIOPHORA-BRASSICAE; ECTOCARPUS-SILICULOSUS; PARASITE; PHAEOPHYCEAE; METABOLISM; INSIGHTS; GENOMES; SUPPORT	Plant-pathogen interactions follow spatial and temporal developmental dynamics where gene expression in pathogen and host undergo crucial changes. Therefore, it is of great interest to detect, quantify and localise where and when key genes are active to understand these processes. Many pathosystems are not accessible for genetic amendments or other spatially-resolved gene expression monitoring methods. Here, we adapt single molecule FISH techniques to demonstrate the presence and activity of mRNAs at the single-cell level using phytomyxids in their plant and algal host in lab and field material. This allowed us to monitor and quantify the expression of genes from the clubroot pathogen Plasmodiophora brassicae, several species of its Brassica hosts, and of several brown algae, including the genome model Ectocarpus siliculosus, infected with the phytomyxid Maullinia ectocarpii. We show that mRNAs are localised along a spatiotemporal gradient, thus providing a proof-of-concept of the usefulness of single-molecule FISH to increase knowledge about the interactions between plants, algae and phytomyxids. The methods used are easily applicable to any interaction between microbes and their algal or plant host, and have therefore the potential to rapidly increase our understanding of key, spatially- and temporally-resolved processes underpinning complex plant-microbe interactions.	[Badstoeber, Julia; Neuhauser, Sigrid] Univ Innsbruck, Inst Microbiol, A-6020 Innsbruck, Austria; [Gachon, Claire M. M.] Scottish Marine Inst, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland; [Gachon, Claire M. M.] CNRS, Museum Natl Hist Nat, UMR Mol Commun & Adaptat Microorganismes 7245, F-75005 Paris, France; [Ludwig-Mueller, Jutta] Tech Univ Dresden, Inst Bot, D-01217 Dresden, Germany; [Sandbichler, Adolf M.] Univ Innsbruck, Inst Zool, A-6020 Innsbruck, Austria	Neuhauser, S (corresponding author), Univ Innsbruck, Inst Microbiol, A-6020 Innsbruck, Austria.	Sigrid.Neuhauser@uibk.ac.at	Neuhauser, Sigrid/C-8189-2014	Neuhauser, Sigrid/0000-0003-0305-1615	Austrian Science Fund (FWF)Austrian Science Fund (FWF) [Y801-B16]; European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant [642575]; UK NERC under the grant agreement GlobalSeaweed [NE/L013223/1]	JB and SN were funded by the Austrian Science Fund (FWF): Grant Y801-B16 (START-grant). CG has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No 642575, and from the UK NERC under the grant agreement GlobalSeaweed (NE/L013223/1). We thank Stefan Ciaghi for providing the transcriptomic data for MEX1. The authors want to thank Martin Kirchmair, Arne Schwelm, Mohammad Etemadi and Stefan Ciaghi for useful discussions.	Archibald JM, 2004, J EUKARYOT MICROBIOL, V51, P113, DOI 10.1111/j.1550-7408.2004.tb00172.x; Baroja-Fernandez E, 2012, P NATL ACAD SCI USA, V109, P321, DOI 10.1073/pnas.1117099109; Bruno L, 2011, INT J DEV BIOL, V55, P197, DOI 10.1387/ijdb.103132lb; Bulman S, 2019, PLANT BIOLOGY, V21, P120, DOI 10.1111/plb.12728; Bulman S, 2006, FEMS MICROBIOL LETT, V264, P198, DOI 10.1111/j.1574-6968.2006.00466.x; Bulman S, 2011, PROTIST, V162, P449, DOI 10.1016/j.protis.2010.09.004; Buxbaum AR, 2015, NAT REV MOL CELL BIO, V16, P95, DOI 10.1038/nrm3918; Cavalier-Smith T, 2003, J MOL EVOL, V56, P540, DOI 10.1007/s00239-002-2424-z; Chen KH, 2015, SCIENCE, V348, DOI 10.1126/science.aaa6090; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; Ding YL, 2014, NATURE, V505, P696, DOI 10.1038/nature12756; Djavaheri M, 2019, MOL PLANT MICROBE IN, V32, P296, DOI 10.1094/MPMI-07-18-0192-R; Donald EC, 2008, PLANT PATHOL, V57, P201, DOI 10.1111/j.1365-3059.2007.01765.x; Duncan S, 2016, PLANT METHODS, V12, DOI 10.1186/s13007-016-0114-x; Egan S, 2013, FEMS MICROBIOL REV, V37, P462, DOI 10.1111/1574-6976.12011; Farnham G, 2013, J PHYCOL, V49, P819, DOI 10.1111/jpy.12096; Fesel PH, 2016, FUNGAL GENET BIOL, V90, P53, DOI 10.1016/j.fgb.2015.12.004; Foley SW, 2017, WIRES RNA, V8, DOI 10.1002/wrna.1426; Francoz E, 2016, SCI REP-UK, V6, DOI 10.1038/srep24644; Gaspar I, 2015, WIRES DEV BIOL, V4, P135, DOI 10.1002/wdev.170; Irani S, 2018, BMC GENOMICS, V19, DOI 10.1186/s12864-017-4426-7; Kemen E, 2012, TRENDS PLANT SCI, V17, P448, DOI 10.1016/j.tplants.2012.04.005; Leblanc C, 2015, COORDIN CHEM REV, V301, P134, DOI 10.1016/j.ccr.2015.02.013; Lee C, 2016, METHODS, V98, P124, DOI 10.1016/j.ymeth.2015.12.007; Libault M, 2017, TRENDS PLANT SCI, V22, P949, DOI 10.1016/j.tplants.2017.08.006; Ludwig-Muller J, 2015, MOL PLANT PATHOL, V16, P349, DOI 10.1111/mpp.12185; Ludwig-Muller J, 2009, J PLANT GROWTH REGUL, V28, P229, DOI 10.1007/s00344-009-9089-4; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; Medioni Caroline, 2018, Methods Mol Biol, V1649, P1, DOI 10.1007/978-1-4939-7213-5_1; Merchante C, 2017, PLANT J, V90, P628, DOI 10.1111/tpj.13520; Misra BB, 2014, TRENDS PLANT SCI, V19, P637, DOI 10.1016/j.tplants.2014.05.005; Moor AE, 2017, CURR OPIN BIOTECH, V46, P126, DOI 10.1016/j.copbio.2017.02.004; Muhlenberg I, 2003, PLANT PROT SCI PRAGU, V38, P69, DOI [10.17221/10323-PPS, DOI 10.17221/10323-PPS]; Muller DB, 2016, ANNU REV GENET, V50, P211, DOI 10.1146/annurev-genet-120215-034952; Murua P, 2017, PROTIST, V168, P468, DOI 10.1016/j.protis.2017.07.001; Neuhauser S, 2011, EUR J PLANT PATHOL, V130, P503, DOI 10.1007/s10658-011-9769-3; Niessing D, 2018, WIRES RNA, V9, DOI 10.1002/wrna.1453; Riascos Donald, 2011, Agron. colomb., V29, P57; Robin A. H. K., 2019, Plant Breeding and Biotechnology, V7, P73, DOI 10.9787/PBB.2019.7.2.73; Rolfe SA, 2016, BMC GENOMICS, V17, DOI 10.1186/s12864-016-2597-2; Schneider CA, 2012, NAT METHODS, V9, P671, DOI 10.1038/nmeth.2089; Schuller A, 2016, PLANT PATHOL, V65, P1223, DOI 10.1111/ppa.12520; Schuller A, 2014, PLANT CELL PHYSIOL, V55, P392, DOI 10.1093/pcp/pct174; Schwelm A, 2018, MOL PLANT PATHOL, V19, P1029, DOI 10.1111/mpp.12580; Schwelm A, 2015, SCI REP-UK, V5, DOI 10.1038/srep11153; Singh K, 2018, MOL GENET GENOMICS, V293, P381, DOI 10.1007/s00438-017-1395-0; Stettler M, 2009, MOL PLANT, V2, P1233, DOI 10.1093/mp/ssp093; Strittmatter M, 2016, PLANT CELL ENVIRON, V39, P259, DOI 10.1111/pce.12533; Trcek T, 2017, NAT PROTOC, V12, P1326, DOI 10.1038/nprot.2017.030; van Gijtenbeek LA, 2017, FRONT MICROBIOL, V8, P1, DOI 10.3389/fmicb.2017.01161; Vandenkoornhuyse P, 2015, NEW PHYTOL, V206, P1196, DOI 10.1111/nph.13312; Vlot A. C., 2017, ELS, DOI [10.1002/9780470015902.a0001322.pub3, DOI 10.1002/9780470015902.A0001322.PUB3]; Wang DJ, 2010, TRENDS BIOTECHNOL, V28, P281, DOI 10.1016/j.tibtech.2010.03.002; Weibrecht I, 2013, NAT PROTOC, V8, P355, DOI 10.1038/nprot.2013.006; West JA, 1999, HYDROBIOLOGIA, V399, P101; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zeilinger S, 2016, FEMS MICROBIOL REV, V40, P182, DOI 10.1093/femsre/fuv045	58	0	0	1	1	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	2045-2322			SCI REP-UK	Sci Rep	AUG 31	2020	10	1							14269	10.1038/s41598-020-70884-4			13	Multidisciplinary Sciences	Science & Technology - Other Topics	NP2UE	WOS:000570035500016	32868853	DOAJ Gold, Green Published			2021-04-07	
J	Simeon, A; Kridi, S; Kloareg, B; Herve, C				Simeon, Amandine; Kridi, Sonia; Kloareg, Bernard; Herve, Cecile			Presence of Exogenous Sulfate Is Mandatory for Tip Growth in the Brown AlgaEctocarpus subulatus	FRONTIERS IN PLANT SCIENCE			English	Article						tip growth; sulfated fucans; sulfate; extracellular matrix; cell wall; brown algae; Ectocarpus; alginate	ALGA ECTOCARPUS-SILICULOSUS; CELL-WALL; ARABINOGALACTAN PROTEINS; FUCUS ZYGOTES; MULTICELLULARITY; IDENTIFICATION; PHAEOPHYCEAE; EVOLUTION; FIXATION; EPITOPES	Brown algae (Phaeophyceae) are multicellular photoautrophic organisms and the largest biomass producers in coastal regions. A variety of observations indicate that their extracellular matrix (ECM) is involved with screening of salts, development, cell fate selection, and defense responses. It is likely that these functionalities are related to its constitutive structures. The major components of the ECM of brown algae are beta-glucans, alginates, and fucose-containing sulfated polysaccharides. The genusEctocarpuscomprises a wide range of species that have adapted to different environments, including isolates ofEctocarpus subulatus, a species highly resistant to low salinity. Previous studies on a freshwater strain ofE. subulatusindicated that the sulfate remodeling of fucans is related to the external salt concentration. Here we show that the sulfate content of the surrounding medium is a key parameter influencing both the patterning of the alga and the occurrence of the BAM4 sulfated fucan epitope in walls of apical cells. These results indicate that sulfate uptake and incorporation in the sulfated fucans from apical cells is an essential parameter to sustain tip growth, and we discuss its influence on the architectural plasticity ofEctocarpus.	[Simeon, Amandine; Kridi, Sonia; Kloareg, Bernard; Herve, Cecile] Sorbonne Univ, CNRS, Integrat Biol Marine Models LBI2M, Stn Biol Roscoff, Roscoff, France	Herve, C (corresponding author), Sorbonne Univ, CNRS, Integrat Biol Marine Models LBI2M, Stn Biol Roscoff, Roscoff, France.	cecile.herve@sb-roscoff.fr			Brittany regionRegion Bretagne [ARED_ 8979 ECTOPAR]; French National Research AgencyFrench National Research Agency (ANR) [ANR-10-BTBR-04]	AS received a grant from the Brittany region (ARED_ 8979 ECTOPAR). CH acknowledges funding from the French National Research Agency with regard to the investment expenditure program Idealg (http://www.idealg.ueb.eu/, grant agreement no. ANR-10-BTBR-04).	Avia K, 2017, SCI REP-UK, V7, DOI 10.1038/srep43241; BERGER F, 1994, SCIENCE, V263, P1421, DOI 10.1126/science.263.5152.1421; Berger F, 1993, ZYGOTE, V1, P9, DOI 10.1017/S0967199400001246; Bisgrove Sherryl R., 2008, V9, P323, DOI 10.1007/7089_2007_134; Bisgrove SR, 2001, PLANTA, V212, P648, DOI 10.1007/s004250000434; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Chebli Y, 2012, PLANT PHYSIOL, V160, P1940, DOI 10.1104/pp.112.199729; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; CRAYTON MA, 1974, DEV BIOL, V39, P164, DOI 10.1016/S0012-1606(74)80018-7; Deniaud-Bouet E, 2017, CARBOHYD POLYM, V175, P395, DOI 10.1016/j.carbpol.2017.07.082; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Dittami SM, 2020, J PHYCOL, V56, P719, DOI 10.1111/jpy.12970; Dittami SM, 2020, MAR GENOM, V52, DOI 10.1016/j.margen.2020.100740; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Fischl R, 2016, GLYCOBIOLOGY, V26, P973, DOI 10.1093/glycob/cww040; Herve C, 2016, NEW PHYTOL, V209, P1428, DOI 10.1111/nph.13786; Katsaros Christos I., 1995, Phycological Research, V43, P43, DOI 10.1111/j.1440-1835.1995.tb00004.x; KLOAREG B, 1986, INT J BIOL MACROMOL, V8, P380, DOI 10.1016/0141-8130(86)90060-7; KROPF DL, 1988, SCIENCE, V239, P187, DOI 10.1126/science.3336780; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Lee KJD, 2005, PLANT CELL, V17, P3051, DOI 10.1105/tpc.105.034413; Marcus SE, 2008, BMC PLANT BIOL, V8, DOI 10.1186/1471-2229-8-60; MEEKES HTHM, 1986, J EXP BOT, V37, P1201, DOI 10.1093/jxb/37.8.1201; MEIKLE PJ, 1991, PLANTA, V185, P1, DOI 10.1007/BF00194507; Parre E, 2005, PLANTA, V220, P582, DOI 10.1007/s00425-004-1368-5; QUATRANO RS, 1976, PLANT PHYSIOL, V58, P224, DOI 10.1104/pp.58.2.224; QUATRANO RS, 1991, DEVELOPMENT, P11; Rabille H, 2019, SCI REP-UK, V9, DOI 10.1038/s41598-019-49427-z; Rabille H, 2019, PLOS BIOL, V17, DOI 10.1371/journal.pbio.2005258; Riquelme M, 2011, FUNGAL BIOL-UK, V115, P446, DOI 10.1016/j.funbio.2011.02.008; Salmean AA, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-03081-5; Tarver JE, 2015, NUCLEIC ACIDS RES, V43, P6384, DOI 10.1093/nar/gkv578; Taylor AR, 1996, PLANT CELL, V8, P2015; Torode TA, 2016, J EXP BOT, V67, P6089, DOI 10.1093/jxb/erw369; Torode TA, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0118366; WAGNER VT, 1992, P NATL ACAD SCI USA, V89, P3644, DOI 10.1073/pnas.89.8.3644	39	0	0	1	2	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-462X			FRONT PLANT SCI	Front. Plant Sci.	AUG 18	2020	11								1277	10.3389/fpls.2020.01277			11	Plant Sciences	Plant Sciences	NJ6SH	WOS:000566173700001	33013948	DOAJ Gold, Green Published			2021-04-07	
J	Munoz, PT; Rodriguez-Rojas, F; Celis-Pla, PSM; Mendez, L; Pinto, D; Pardo, D; Moenne, F; Sanchez-Lizaso, JL; Saez, CA				Munoz, Pamela T.; Rodriguez-Rojas, Fernanda; Celis-Pla, Paula S. M.; Mendez, Lorena; Pinto, Denise; Pardo, Diego; Moenne, Fabiola; Luis Sanchez-Lizaso, Jose; Saez, Claudio A.			Physiological and metabolic responses to hypersalinity reveal interpopulation tolerance in the green macroalga Ulva compressa with different pollution histories	AQUATIC TOXICOLOGY			English	Article						Osmotic stress; Chlorophyta; Desalination; Reactive oxygen species; Transcriptomics; Intraspecific	ANTIOXIDANT DEFENSE SYSTEM; INDUCED OXIDATIVE STRESS; CHLOROPHYLL FLUORESCENCE; ECTOCARPUS-SILICULOSUS; POSIDONIA-OCEANICA; GENE-EXPRESSION; SALINITY STRESS; GLUTATHIONE; ASCORBATE; ACCLIMATION	There is scarce investigation addressing interpopulation tolerance responses to address the influence of a history of chronic stress exposure, as that occurring in polluted environments, in photoautotrophs. We evaluated ecophysiological (photosynthetic activity) and metabolic (oxidative stress and damage) responses of two populations of green macroalga Ulva compressa from polluted (Ventanas) and non-polluted (Cachagua) localions of central Chile, and exposed to controlled hypersalinity conditions of 32 (control), 42, 62 and 82 psu (practical salinity units) for 6 h, 48 h and 6 d. Both primary production (ETRmax) and photosynthetic efficiency (alpha(ETR)) were generally higher in the population from Cachagua compared to Ventanas at all times and salinities. Moreover, at most experimental times and salinities the population from Ventanas had greater levels of H2O2 and lipid peroxidation that individuals from Cachagua. Total ascorbate was higher in the population of Cachagua than Ventanas at 42 and 82 psu after 6 and 48 h, respectively, while at 6 d concentrations were similar between both populations at all salinities. Total glutathione was greater in both populations after 6 h at all salinities, but at 48 h its concentrations were higher only in the population from Cachagua, a trend that was maintained at 6 d under 82 psu only. Reduced and oxidized ascorbate (ASC and DHA, respectively) and glutathione (GSH and GSSG, respectively) demonstrated similar patterns between U. compressa populations, with an increase oxidation with greater salinities but efficient recycling to maintain sufficient batch of ASC and GSH. When assessing the expression of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and dehydroascorbate reductase (DHAR), while the population of Ventanas displayed a general trend of upregulation with increasing salinities along the experiments, U. compressa from Cachagua revealed patterns of downregulation. Results demonstrated that although both populations were still viable after the applied hypersalinities during all experimental times, biological performance was usually more affected in the population from the Ventanas than Cachagua, likely due to a depressed baseline metabolism after a long history of exposition to environmental pollution.	[Munoz, Pamela T.; Rodriguez-Rojas, Fernanda; Celis-Pla, Paula S. M.; Mendez, Lorena; Pinto, Denise; Pardo, Diego; Moenne, Fabiola; Saez, Claudio A.] Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar, Chile; [Munoz, Pamela T.; Pinto, Denise] Univ Playa Ancha, Fac Ciencias Nat & Exactas, Doctorado Interdisciplinario Ciencias Ambientales, Valparaiso, Chile; [Munoz, Pamela T.] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, Doctorado Ciencias Mar & Biol Aplicada, Alicante, Spain; [Mendez, Lorena] Univ Valparaiso, Fac Ciencias Mar & Recursos Nat, Carrera Biol Marina, Vina Del Mar, Chile; [Pardo, Diego] Univ Playa Ancha, Fac Ingn, Carrera Ingn Civil Ambiental, Valparaiso, Chile; [Luis Sanchez-Lizaso, Jose] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, Alicante, Spain; [Munoz, Pamela T.; Rodriguez-Rojas, Fernanda; Celis-Pla, Paula S. M.; Pinto, Denise; Pardo, Diego; Moenne, Fabiola; Saez, Claudio A.] Univ Playa Ancha, ENVIRONM HUB UPLA, Valparaiso, Chile	Saez, CA (corresponding author), Univ Playa Ancha, ENVIRONM HUB UPLA, Valparaiso, Chile.	claudio.saez@upla.cl	Lizaso, Jose Luis L Sanchez/J-4939-2017; Saez, Claudio/F-5978-2015	Lizaso, Jose Luis L Sanchez/0000-0002-3927-5699; Saez, Claudio/0000-0002-5037-3484	DGI Regular [CEA 01-1819, CEA 19-20]; CORFO [19CTIGH-121349]; FONDECYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [3180394]	The authors thank financial support of projects DGI RegularCEA 01-1819 and CEA 19-20, CORFO 19CTIGH-121349, and FONDECYT Postdoctorado3180394.	Aranda PS, 2012, ELECTROPHORESIS, V33, P366, DOI 10.1002/elps.201100335; Asada K, 1999, ANNU REV PLANT PHYS, V50, P601, DOI 10.1146/annurev.arplant.50.1.601; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; Celis-Pla PSM, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20184547; Duran RE, 2019, FRONT MICROBIOL, V10, DOI 10.3389/fmicb.2019.00528; EILERS PHC, 1988, ECOL MODEL, V42, P199, DOI 10.1016/0304-3800(88)90057-9; Fernandez-Torquemada Y, 2009, DESALIN WATER TREAT, V5, P137, DOI 10.5004/dwt.2009.576; Figueroa FL, 2014, MAR ENVIRON RES, V97, P30, DOI 10.1016/j.marenvres.2014.01.009; Figueroa FL, 2003, PHOTOSYNTH RES, V75, P259, DOI 10.1023/A:1023936313544; Foyer CH, 2011, PLANT PHYSIOL, V155, P2, DOI 10.1104/pp.110.167569; Garrote-Moreno A, 2015, AQUAT BOT, V120, P315, DOI 10.1016/j.aquabot.2014.09.011; Gill SS, 2010, PLANT PHYSIOL BIOCH, V48, P909, DOI 10.1016/j.plaphy.2010.08.016; Hajiboland R, 2014, OXIDATIVE DAMAGE TO PLANTS: ANTIOXIDANT NETWORKS AND SIGNALING, P1, DOI 10.1016/B978-0-12-799963-0.00001-0; Imlay JA, 2003, ANNU REV MICROBIOL, V57, P395, DOI 10.1146/annurev.micro.57.030502.090938; Karanov, 1997, P B ACAD SCI, V51, P121; KIRST GO, 1990, ANNU REV PLANT PHYS, V41, P21, DOI 10.1146/annurev.pp.41.060190.000321; Kumar M, 2014, ADV BOT RES, V71, P91, DOI 10.1016/B978-0-12-408062-1.00004-4; Li YF, 2016, MAR BIOL RES, V12, P631, DOI 10.1080/17451000.2016.1177654; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Lu IF, 2006, MAR BIOL, V150, P1, DOI 10.1007/s00227-006-0323-3; Luo MB, 2011, J EXP MAR BIOL ECOL, V409, P223, DOI 10.1016/j.jembe.2011.08.023; Marin-Guirao L, 2013, MAR ENVIRON RES, V84, P60, DOI 10.1016/j.marenvres.2012.12.001; Mellado M, 2012, PLANT PHYSIOL BIOCH, V51, P102, DOI 10.1016/j.plaphy.2011.10.007; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Neaman A, 2009, GEODERMA, V150, P359, DOI 10.1016/j.geoderma.2009.02.017; Noctor G, 2002, J EXP BOT, V53, P1283, DOI 10.1093/jexbot/53.372.1283; Ogawa T, 2013, PHYCOLOGIA, V52, P637, DOI 10.2216/13-199.1; Panda A, 2019, ENVIRON EXP BOT, V166, DOI 10.1016/j.envexpbot.2019.103799; Ratkevicius N, 2003, PLANT CELL ENVIRON, V26, P1599, DOI 10.1046/j.1365-3040.2003.01073.x; Rodriguez-Rojas F, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20184546; Ruiz JM, 2002, PLANTA, V214, P965, DOI 10.1007/s00425-002-0748-y; Rybak AS, 2018, ECOL INDIC, V85, P253, DOI 10.1016/j.ecolind.2017.10.061; Saez CA, 2015, PHYCOLOGIA, V54, P425, DOI 10.2216/15-30.1; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Saez CA, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0050170; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; Sampath-Wiley P, 2008, J EXP MAR BIOL ECOL, V361, P83, DOI 10.1016/j.jembe.2008.05.001; Sandoval-Gil JM, 2014, MAR ENVIRON RES, V95, P39, DOI 10.1016/j.marenvres.2013.12.011; Schreiber U., 1995, ECOPHYSIOLOGY PHOTOS, P49, DOI [DOI 10.1007/978-3-642-79354-7_3, 10.1007/978-3-642-79354-7_3]; Sievers Hellmuth A., 2000, Revista de Biologia Marina y Oceanografia, V35, P153; Slesak I, 2016, ASTROBIOLOGY, V16, P348, DOI 10.1089/ast.2015.1328; Sola I, 2019, WATER-SUI, V11, DOI 10.3390/w11102085; Sola I, 2019, DESALINATION, V471, DOI 10.1016/j.desal.2019.114132; Sung MS, 2009, MAR BIOTECHNOL, V11, P199, DOI 10.1007/s10126-008-9134-5; Tine M, 2008, MAR GENOM, V1, P37, DOI 10.1016/j.margen.2008.06.001; Tume P., 2019, INFLUENCE IND AREA P; Vargas-Chacoff L, 2015, FISH PHYSIOL BIOCHEM, V41, P1369, DOI 10.1007/s10695-015-0092-3; Wang WL, 2019, SCI TOTAL ENVIRON, V662, P168, DOI 10.1016/j.scitotenv.2019.01.214; Wang Y, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0038245; Xia JR, 2004, AQUAT BOT, V80, P129, DOI 10.1016/j.aquabot.2004.07.006	51	1	1	3	7	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0166-445X	1879-1514		AQUAT TOXICOL	Aquat. Toxicol.	AUG	2020	225								105552	10.1016/j.aquatox.2020.105552			9	Marine & Freshwater Biology; Toxicology	Marine & Freshwater Biology; Toxicology	MO6JU	WOS:000551630500020	32615475				2021-04-07	
J	Landa-Cansigno, C; Hernandez-Dominguez, EE; Monribot-Villanueva, JL; Licea-Navarro, AF; Mateo-Cid, LE; Segura-Cabrera, A; Guerrero-Analco, JA				Landa-Cansigno, Cristina; Hernandez-Dominguez, Eric E.; Monribot-Villanueva, Juan L.; Licea-Navarro, Alexei F.; Mateo-Cid, Luz E.; Segura-Cabrera, Aldo; Guerrero-Analco, Jose A.			Screening of Mexican tropical seaweeds as sources of alpha-amylase and alpha- glucosidase inhibitors	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS			English	Article						Chlorophyta; Hyperglycemia; Metabolic syndrome; Metabolomics; Ochrophyta; Rhodophyta	MARINE MACROALGAE; GREEN-ALGAE; BROWN-ALGAE; IN-VITRO; PHLOROTANNINS; RED; POLYPHENOLS; INSIGHTS	A key therapeutic strategy to prevent metabolic syndrome is the inhibition of alpha-amylase and alpha-glucosidase. Derivatives isolated from naturally-sourced seaweeds may act as inhibitors of these enzymes. The aims of this study are to evaluate in vitro the alpha-amylase and alpha-glucosidase inhibition of 45 crude extracts from 31 species of Ochrophyta, Rhodophyta, and Chlorophyta present in Mexican seashores, describe their acute toxicity, and putatively identify some of the potential bioactive compounds by using untargeted metabolomics. Also, active extracts were evaluated in the brine shrimp lethality test. Samples were collected during rainy and dry seasons in the rocky shores of Paraiso, Villa Rica and Munecos, in Veracruz, Mexico. Crude extracts were obtained by maceration and then tested on both enzymes. Chemical profiling was done by accurate mass spectrometry and data was analyzed using statistical tools. The results showed that seaweeds from Veracruz are sources of aamylase and alpha-glucosidase inhibitors. The highest alpha-amylase and a-glucosidase inhibition (IC50 values) were observed in Cladophora dalmatica (116.99 +/- 11.59, 27.86 +/- 2.95 mu g mL(-1)), Ectocarpus siliculosus (679 +/- 68.17, 276.86 +/- 11.20 mu g mL(-1)), Padina boergesenii (567.01 +/- 65.20, 43.89 +/- 5.46 mu g mL(-1)) and P. gymnospora (> 1000, 59.92 +/- 7.45 mu g mL(-1)) species, respectively. Active extracts were more effective inhibitors of alpha-glucosidase compared to acarbose (> 1000 mu g mL(-1)), used as drug reference. C. dalmatica showed high toxicity (LC50 = 37.55 +/- 1.04 mu g mL(-1)), whilst the rest of the active extracts did not. Fatty acids and terpenoids were tentatively identified in the active extracts as potential inhibitors of tested enzymes. In conclusion, Mexican seaweeds constitute sources of metabolites that could reduce hyperglycemia postprandial by the inhibition of alpha-amylase and a-glucosidase. Ochrophyta species are the best sources to look for these inhibitors because their extracts are not toxic and displayed lower alpha-amylase inhibitory activities.	[Landa-Cansigno, Cristina; Hernandez-Dominguez, Eric E.; Monribot-Villanueva, Juan L.; Guerrero-Analco, Jose A.] Inst Ecol AC, Lab Quim Prod Nat, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, Xalapa 91073, Veracruz, Mexico; [Hernandez-Dominguez, Eric E.] Inst Ecol AC, Lab Prote, Catedra CONACYT, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, Xalapa 91073, Veracruz, Mexico; [Licea-Navarro, Alexei F.] Ctr Invest Cient & Estudios Super Ensenada, Dept Innovac Biomed, Carretera Tijuana Ensenada 3918, Ensenada 22860, Baja California, Mexico; [Mateo-Cid, Luz E.] Inst Politecn Nacl, Dept Bot, Lab Ficol, Carpio & Plan Ayala S-N, Mexico City 11340, DF, Mexico; [Segura-Cabrera, Aldo] European Bioinformat Inst, European Mol Biol Lab, Hixton CB10 1SD, Cambs, England	Guerrero-Analco, JA (corresponding author), Inst Ecol AC, Lab Quim Prod Nat, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, Xalapa 91073, Veracruz, Mexico.	joseantonio.guerrero@inecol.mx	Licea, Alexei/B-4106-2016	Licea, Alexei/0000-0003-4022-7405; Hernandez Dominguez, Eric Edmundo/0000-0002-1153-9592; Mateo-Cid, Luz Elena/0000-0001-7585-4826	Instituto de Ecologia A.C., Mexico [20030, 11408, 2016-2]; Consejo Nacional de Ciencia y Tecnologia, MexicoConsejo Nacional de Ciencia y Tecnologia (CONACyT) [300044]	This work was supported by the Instituto de Ecologia A.C., Mexico (projects 20030, 2016; 11408, 2017; mobility grant 2016-2, 2016). PhD. scholarship to C.L.C by the Consejo Nacional de Ciencia y Tecnologia, Mexico (300044, 2016-2020).	Alali FQ, 2006, NAT PROD RES, V20, P558, DOI 10.1080/14786410500183381; Ali L, 2017, MAR DRUGS, V15, DOI 10.3390/md15010019; Ali L, 2015, MAR DRUGS, V13, P4344, DOI 10.3390/md13074344; Ali MY, 2017, MAR DRUGS, V15, DOI 10.3390/md15120368; Amsler C. D., 2008, P91; Apostolidis E, 2010, J FOOD SCI, V75, pH97, DOI 10.1111/j.1750-3841.2010.01544.x; Ara J, 1999, PHYTOTHER RES, V13, P304, DOI 10.1002/(SICI)1099-1573(199906)13:4&lt;304::AID-PTR439&gt;3.0.CO;2-9; Asp NG, 1996, FOOD CHEM, V57, P9, DOI 10.1016/0308-8146(96)00055-6; Ayesha, 2010, PAK J BOT, V42, P3555; Belghit I, 2017, ALGAL RES, V26, P240, DOI 10.1016/j.algal.2017.08.001; BISCHOFF H, 1994, EUR J CLIN INVEST, V24, P3; Borcard D, 2011, USE R, P1, DOI 10.1007/978-1-4419-7976-6; Carballo Jose Luis, 2002, BMC Biotechnol, V2, P17, DOI 10.1186/1472-6750-2-17; CAVALIERSMITH T, 1991, TRENDS GENET, V7, P145, DOI 10.1016/0168-9525(91)90377-3; Chin YX, 2015, J APPL PHYCOL, V27, P2137, DOI 10.1007/s10811-014-0462-8; Chmielewska E, 2001, CROAT CHEM ACTA, V74, P135; Colegate S.M., 2007, BIOACTIVE NATURAL PR, DOI [10.1016/s0031-9422(97)87094-x, DOI 10.1016/S0031-9422(97)87094-X]; Collins KG, 2016, MAR DRUGS, V14, DOI 10.3390/md14030060; Crawley M.J., 2013, R BOOK, DOI [10.1086/431086, DOI 10.1086/431086]; Gestinari LMD, 2010, PHYTOTAXA, V14, P22, DOI 10.11646/phytotaxa.14.1.2; Eom SH, 2012, J SCI FOOD AGR, V92, P2084, DOI 10.1002/jsfa.5585; FAO, 2018, GLOB STAT SEAW PROD, V124, P120; Faridmoayer A, 2005, GLYCOBIOLOGY, V15, P1341, DOI 10.1093/glycob/cwj009; FRANCISCO C, 1985, TETRAHEDRON LETT, V26, P4919, DOI 10.1016/S0040-4039(00)94985-2; Geissert-Kientz D., 1999, INVESTIGACIONES GEOG, V40, P23; Gella FJ, 1997, CLIN CHIM ACTA, V259, P147, DOI 10.1016/S0009-8981(96)06481-9; GERWICK WH, 1979, TETRAHEDRON LETT, P145; Goodman BE, 2010, ADV PHYSIOL EDUC, V34, P44, DOI 10.1152/advan.00094.2009; Gurgel CFD, 2004, CRYPTOGAMIE ALGOL, V25, P367; Kellogg J, 2014, MAR DRUGS, V12, P5277, DOI 10.3390/md12105277; Kim KY, 2008, PHYTOCHEMISTRY, V69, P2820, DOI 10.1016/j.phytochem.2008.09.007; Kim KY, 2010, J FOOD SCI, V75, pH145, DOI 10.1111/j.1750-3841.2010.01629.x; Koh LW, 2010, J AGR FOOD CHEM, V58, P148, DOI 10.1021/jf903011g; Kumar M, 2016, ALGAL RES, V16, P76, DOI 10.1016/j.algal.2016.02.033; Lakshmanasenthil S., 2013, INT J PHAR PHAR SC, V5, P240; Landa-Cansigno C, 2019, ACTA BOT MEX, V126, DOI 10.21829/abm126.2019.1525; Lauritano C, 2016, MAR DRUGS, V14, DOI 10.3390/md14120220; Lavelli V, 2017, INNOV FOOD SCI EMERG, V39, P156, DOI 10.1016/j.ifset.2016.12.006; Lee Chae-Won, 2012, Prev Nutr Food Sci, V17, P8, DOI 10.3746/pnf.2012.17.1.008; Lee SH, 2013, FITOTERAPIA, V86, P129, DOI 10.1016/j.fitote.2013.02.013; Lee SH, 2010, FOOD CHEM TOXICOL, V48, P2633, DOI 10.1016/j.fct.2010.06.032; Littler DS., 2000, CARIBBEAN REEF PLANT; Lordan S, 2013, FOOD CHEM, V141, P2170, DOI 10.1016/j.foodchem.2013.04.123; MacGregor EA, 2001, BBA-PROTEIN STRUCT M, V1546, P1, DOI 10.1016/S0167-4838(00)00302-2; Mahmood N., 2016, Comparative Clinical Pathology, V25, P1253, DOI 10.1007/s00580-014-1967-x; Meenakshi S.K., 2018, RES REV J LIFE SCI, V8, P24; MEYER BN, 1982, PLANTA MED, V45, P31, DOI 10.1055/s-2007-971236; Mohanapriya V., 2016, SAUDI J BIOMED RES, V1, P59, DOI [10.21276/sjbr.2016.1.3.1, DOI 10.21276/SJBR.2016.1.3.1]; Moon HE, 2011, BIOSCI BIOTECH BIOCH, V75, P1472, DOI 10.1271/bbb.110137; Murugan AC, 2015, PHARM BIOL, V53, P1087, DOI 10.3109/13880209.2014.959615; Murugesan S., 2016, WORLD J PHARM SCI, V4, P415; Muzquiz de la Garza Ana Rocio, 2019, Biomed Res Int, V2019, P3795160, DOI 10.1155/2019/3795160; Park Mi Hwa, 2012, Prev Nutr Food Sci, V17, P239, DOI [10.3746/pnf.2012.17.4.239, 10.3746/pnf.2012.17.4.]; Paul J. P., 2014, INT J INNNOVATIVE DR, V4, P164; Pedroche Francisco F., 2003, Hidrobiológica, V13, P23; Pirian K, 2017, J APPL PHYCOL, V29, P3151, DOI 10.1007/s10811-017-1152-0; Ritz C, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0146021; Sharifuddin Y, 2015, MAR DRUGS, V13, P5447, DOI 10.3390/md13085447; Slobodnick A, 2015, AM J MED, V128, P461, DOI 10.1016/j.amjmed.2014.12.010; Stengel DB, 2015, METHODS MOL BIOL, V1308, P1, DOI 10.1007/978-1-4939-2684-8_1; Stengel DB, 2011, BIOTECHNOL ADV, V29, P483, DOI 10.1016/j.biotechadv.2011.05.016; Su CH, 2013, BIOFACTORS, V39, P415, DOI 10.1002/biof.1082; Taylor WR, 1960, MARINE ALGAE E TROPI; Teixeira VL, 2007, FITOTERAPIA, V78, P35, DOI 10.1016/j.fitote.2006.09.017; Trease G.E., 2002, TREASE EVANS PHARMAC, DOI [10.1017/CBO9781107415324, DOI 10.1017/CBO9781107415324.004]; Triana-Mantilla M.E., 2001, REV CUB ANGIOL CIR V, V2, P131; Unnikrishnan PS, 2015, PHARMACOGN MAG, V11, pS511, DOI 10.4103/0973-1296.172954; Whitcomb DC, 2007, DIGEST DIS SCI, V52, P1, DOI 10.1007/s10620-006-9589-z; WHO, 2013, GLOB ACT PLAN PREV C, V92, P102; WHO, 2018, NONC DIS, V157, DOI [10.1002/9781119097136.part5, DOI 10.1002/9781119097136]	70	2	2	5	8	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	2211-9264			ALGAL RES	Algal Res.	AUG	2020	49								101954	10.1016/j.algal.2020.101954			11	Biotechnology & Applied Microbiology	Biotechnology & Applied Microbiology	MF7XS	WOS:000545552200013					2021-04-07	
J	Dittami, SM; Corre, E; Brillet-Gueguen, L; Lipinska, AP; Pontoizeau, N; Aite, M; Avia, K; Caron, C; Cho, CH; Collen, J; Cormier, A; Delage, L; Doubleau, S; Frioux, C; Gobet, A; Gonzalez-Navarrete, I; Groisillier, A; Herve, C; Jollivet, D; KleinJan, H; Leblanc, C; Liu, X; Marie, D; Markov, GV; Minoche, AE; Monsoor, M; Pericard, P; Perrineau, MM; Peters, AF; Siegel, A; Simeon, A; Trottier, C; Yoon, HS; Himmelbauer, H; Boyen, C; Tonon, T				Dittami, Simon M.; Corre, Erwan; Brillet-Gueguen, Loraine; Lipinska, Agnieszka P.; Pontoizeau, Noe; Aite, Meziane; Avia, Komlan; Caron, Christophe; Cho, Chung Hyun; Collen, Jonas; Cormier, Alexandre; Delage, Ludovic; Doubleau, Sylvie; Frioux, Clemence; Gobet, Angelique; Gonzalez-Navarrete, Irene; Groisillier, Agnes; Herve, Cecile; Jollivet, Didier; KleinJan, Hetty; Leblanc, Catherine; Liu, Xi; Marie, Dominique; Markov, Gabriel, V; Minoche, Andre E.; Monsoor, Misharl; Pericard, Pierre; Perrineau, Marie-Mathilde; Peters, Akira F.; Siegel, Anne; Simeon, Amandine; Trottier, Camille; Yoon, Hwan Su; Himmelbauer, Heinz; Boyen, Catherine; Tonon, Thierry			The genome of Ectocarpus subulatus - A highly stress-tolerant brown alga	MARINE GENOMICS			English	Article							PHYLOGENETIC ANALYSIS; METABOLIC NETWORK; DRAFT GENOME; SILICULOSUS; SEQUENCE; ANNOTATION; PHAEOPHYCEAE; EVOLUTION; MARINE; DIVERSITY	Brown algae are multicellular photosynthetic stramenopiles that colonize marine rocky shores worldwide. Ectocarpus sp. Ec32 has been established as a genomic model for brown algae. Here we present the genome and metabolic network of the closely related species, Ectocarpus subulatus Kiitzing, which is characterized by high abiotic stress tolerance. Since their separation, both strains show new traces of viral sequences and the activity of large retrotransposons, which may also be related to the expansion of a family of chlorophyll-binding proteins. Further features suspected to contribute to stress tolerance include an expanded family of heat shock proteins, the reduction of genes involved in the production of halogenated defence compounds, and the presence of fewer cell wall polysaccharide-modifying enzymes. Overall, E. subulatus has mainly lost members of gene families down-regulated in low salinities, and conserved those that were up-regulated in the same condition. However, 96% of genes that differed between the two examined Ectocarpus species, as well as all genes under positive selection, were found to encode proteins of unknown function. This underlines the uniqueness of brown algal stress tolerance mechanisms as well as the significance of establishing E. subulatus as a comparative model for future functional studies.	[Dittami, Simon M.; Brillet-Gueguen, Loraine; Lipinska, Agnieszka P.; Pontoizeau, Noe; Avia, Komlan; Collen, Jonas; Cormier, Alexandre; Delage, Ludovic; Gobet, Angelique; Groisillier, Agnes; Herve, Cecile; KleinJan, Hetty; Leblanc, Catherine; Markov, Gabriel, V; Perrineau, Marie-Mathilde; Simeon, Amandine; Boyen, Catherine; Tonon, Thierry] Sorbonne Univ, Stn Biol Roscoff, Integrat Biol Marine Models LBI2M, CNRS, F-29680 Roscoff, France; [Corre, Erwan; Brillet-Gueguen, Loraine; Pontoizeau, Noe; Caron, Christophe; Liu, Xi; Monsoor, Misharl; Pericard, Pierre] Sorbonne Univ, Stn Biol Roscoff, ABiMS Platform, FR2424,CNRS, F-29680 Roscoff, France; [Aite, Meziane; Frioux, Clemence; Siegel, Anne; Trottier, Camille] Univ Rennes, IRISA, CNRS, INRIA, F-35000 Rennes, France; [Avia, Komlan] Univ Strasbourg, INRA, UMR A 1131, SVQV, F-68000 Colmar, France; [Cho, Chung Hyun; Yoon, Hwan Su] Sungkyunkwan Univ, Dept Biol Sci, Suwon 16419, South Korea; [Doubleau, Sylvie] UMR DIADE, IRD, 911 Ave Agropolis,BP 64501, F-34394 Montpellier, France; [Gonzalez-Navarrete, Irene; Minoche, Andre E.; Himmelbauer, Heinz] Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Dr Aiguader 88, Barcelona 08003, Spain; [Jollivet, Didier; Marie, Dominique] Sorbonne Univ, Stn Biol Roscoff SBR, Adaptat & Divers Marine Environm ADME, CNRS, F-29680 Roscoff, France; [Minoche, Andre E.; Himmelbauer, Heinz] Max Planck Inst Mol Genet, D-14195 Berlin, Germany; [Perrineau, Marie-Mathilde] Scottish Assoc Marine Sci, Scottish Marine Inst, Oban PA37 1QA, Argyll, Scotland; [Peters, Akira F.] Bezhin Rosko, 40 Rue Pecheurs, F-29250 Santec, France; [Trottier, Camille] Univ Nantes, Lab Digital Sci Nantes LS2N, Nantes, France; [Himmelbauer, Heinz] Univ Nat Resources & Life Sci BOKU, Dept Biotechnol, A-1190 Vienna, Austria; [Tonon, Thierry] Univ York, Ctr Novel Agr Prod, Dept Biol, York YO10 5DD, N Yorkshire, England	Dittami, SM (corresponding author), Sorbonne Univ, Stn Biol Roscoff, Integrat Biol Marine Models LBI2M, CNRS, F-29680 Roscoff, France.	simon.dittami@sb-roscoff.fr	corre, erwan/O-4669-2019; Cho, Chung Hyun/AAM-1233-2020; Pericard, Pierre/AAH-1451-2019; Frioux, Clemence/A-1517-2019; Avia, Komlan/E-6850-2015; Perrineau, Marie-Mathilde/J-4459-2014; Gobet, Angelique/B-7572-2013; Tonon, Thierry/A-3214-2009	corre, erwan/0000-0001-6354-2278; Cho, Chung Hyun/0000-0003-3105-8750; Pericard, Pierre/0000-0001-8167-6448; Frioux, Clemence/0000-0003-2114-0697; Avia, Komlan/0000-0001-6212-6774; Perrineau, Marie-Mathilde/0000-0002-1772-7009; Cormier, Alexandre/0000-0002-7775-8413; Markov, Gabriel V./0000-0002-8566-7482; Gobet, Angelique/0000-0003-4204-8451; Tonon, Thierry/0000-0002-1454-6018	ANR project IDEALGFrench National Research Agency (ANR) [ANR-10-BTBR-04]; European Union's Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie grant [624575]; CNRS Momentum call	We would like to thank Dieter G. Muller for isolating, maintaining, and providing the strain, Philippe Potin, Mark Cock, Susanna Coelho, Florian Maumus, and Olivier Panaud for helpful discussions, as well as Gwendoline Andres for help setting up the Jbrowse instance. This work was funded partially by ANR project IDEALG (ANR-10-BTBR-04) "Investissements d'Avenir, Biotechnologies-Bioressources", the European Union's Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie grant agreement number 624575 (ALFF), and the CNRS Momentum call. Sequencing was performed at the Genomics Unit of the Centre for Genomic Regulation (CRG), Barcelona, Spain.	Aite M, 2018, PLOS COMPUT BIOL, V14, DOI 10.1371/journal.pcbi.1006146; Amtmann A, 2009, MOL PLANT, V2, P3, DOI 10.1093/mp/ssn094; Archibald JM, 2009, CURR BIOL, V19, pR81, DOI 10.1016/j.cub.2008.11.067; Avia K, 2017, SCI REP-UK, V7, DOI 10.1038/srep43241; Bernt M, 2013, MOL PHYLOGENET EVOL, V69, P313, DOI 10.1016/j.ympev.2012.08.023; Boetzer M, 2011, BIOINFORMATICS, V27, P578, DOI 10.1093/bioinformatics/btq683; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Bosi E, 2015, BIOINFORMATICS, V31, P2443, DOI 10.1093/bioinformatics/btv171; Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Cabanettes F, 2018, PEERJ, V6, DOI 10.7717/peerj.4958; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Dal'Molin CGD, 2010, PLANT PHYSIOL, V152, P579, DOI 10.1104/pp.109.148817; Dassanayake M, 2011, NAT GENET, V43, P913, DOI 10.1038/ng.889; Davison IR, 1996, J PHYCOL, V32, P197, DOI 10.1111/j.0022-3646.1996.00197.x; de Boer JG, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-422; Delage L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0019540; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Tommaso P, 2011, NUCLEIC ACIDS RES, V39, pW13, DOI 10.1093/nar/gkr245; Dittami SM, 2017, PEERJ, V5, DOI 10.7717/peerj.4073; Dittami SM, 2017, J PHYCOL, V53, P731, DOI 10.1111/jpy.12547; Dittami SM, 2012, GENOME BIOL, V13, DOI 10.1186/gb-2012-13-8-166; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Dittami SM, 2010, BMC EVOL BIOL, V10, DOI 10.1186/1471-2148-10-365; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dong HP, 2016, BMC GENOMICS, V17, DOI 10.1186/s12864-016-3335-5; Dunn NA, 2019, PLOS COMPUT BIOL, V15, DOI 10.1371/journal.pcbi.1006790; Ellinghaus D, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-18; Emms DM, 2015, GENOME BIOL, V16, DOI 10.1186/s13059-015-0721-2; Eren AM, 2015, PEERJ, V3, DOI 10.7717/peerj.1319; Flutre T, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0016526; Foissac S, 2008, CURR BIOINFORM, V3, P87, DOI 10.2174/157489308784340702; Food and Agriculture Organization of the United Nations F, 2016, GLOB PROD STAT 1950; Goff SA, 2011, FRONT PLANT SCI, V2, DOI 10.3389/fpls.2011.00034; Gotz S, 2008, NUCLEIC ACIDS RES, V36, P3420, DOI 10.1093/nar/gkn176; Gruber A, 2015, PLANT J, V81, P519, DOI 10.1111/tpj.12734; Gualtieri T, 2004, YEAST, V21, P1107, DOI 10.1002/yea.1155; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Harvey W.H., 1848, PHYCOLOGIA BRITANNIC; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hu TT, 2011, NAT GENET, V43, P476, DOI 10.1038/ng.807; Karp PD, 2016, BRIEF BIOINFORM, V17, P877, DOI 10.1093/bib/bbv079; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Khalturin K, 2009, TRENDS GENET, V25, P404, DOI 10.1016/j.tig.2009.07.006; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]; KUTZING F. T., 1843, PHYCOLOGIA GEN ANATO; Lagesen K, 2007, NUCLEIC ACIDS RES, V35, P3100, DOI 10.1093/nar/gkm160; Laslett D, 2004, NUCLEIC ACIDS RES, V32, P11, DOI 10.1093/nar/gkh152; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; Lee E, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-8-r93; Lipinska AP, 2019, GENOME BIOL, V20, DOI 10.1186/s13059-019-1630-6; Lipinska AP, 2016, BMC EVOL BIOL, V16, DOI 10.1186/s12862-015-0577-9; Liu MJ, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms6315; Loira N, 2015, J BIOINF COMPUT BIOL, V13, DOI 10.1142/S0219720015500067; Luo RB, 2012, GIGASCIENCE, V1, DOI 10.1186/2047-217X-1-18; Ma T, 2013, NAT COMMUN, V4, DOI 10.1038/ncomms3797; Mchugh D. J., 2003, FAO FISHERIES TECHNI; Meyer F, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-386; Montecinos AE, 2017, J PHYCOL, V53, P17, DOI 10.1111/jpy.12452; Mosavi LK, 2004, PROTEIN SCI, V13, P1435, DOI 10.1110/ps.03554604; Muller D.G., 1998, VIRUSES MARINE BROWN, P49; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; Nguyen VH, 2009, BMC BIOINFORMATICS, V10, DOI 10.1186/1471-2105-10-329; Nielsen H, 2017, METHODS MOL BIOL, V1611, P59, DOI 10.1007/978-1-4939-7015-5_6; Nishitsuji K, 2019, SCI REP-UK, V9, DOI 10.1038/s41598-019-40955-2; Nishitsuji K, 2016, DNA RES, V23, P561, DOI 10.1093/dnares/dsw039; Oh DH, 2012, GENOME BIOL, V13, DOI [10.1186/gb4003, 10.1186/gb-2012-13-3-241]; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2015, CRYPTOGAMIE ALGOL, V36, P3, DOI 10.7872/crya.v36.iss1.2015.3; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Prigent S, 2017, PLOS COMPUT BIOL, V13, DOI 10.1371/journal.pcbi.1005276; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; Rao A.Q., 2016, PLANT GENOMICS, P273; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Rival A, 2013, PLANT CELL REP, V32, P359, DOI 10.1007/s00299-012-1369-y; Schattner P, 2005, NUCLEIC ACIDS RES, V33, pW686, DOI 10.1093/nar/gki366; Simao FA, 2015, BIOINFORMATICS, V31, P3210, DOI 10.1093/bioinformatics/btv351; Skinner ME, 2009, GENOME RES, V19, P1630, DOI 10.1101/gr.094607.109; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; Suyama M, 2006, NUCLEIC ACIDS RES, V34, pW609, DOI 10.1093/nar/gkl315; Talavera G, 2007, SYST BIOL, V56, P564, DOI 10.1080/10635150701472164; Tan SJ, 2016, GENOME RES, V26, P1663, DOI 10.1101/gr.204925.116; Tang HB, 2015, GENOME BIOL, V16, DOI 10.1186/s13059-014-0573-1; Torode TA, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0118366; Waterhouse AM, 2009, BIOINFORMATICS, V25, P1189, DOI 10.1093/bioinformatics/btp033; West John A., 1996, Muelleria, V9, P29; Wu HJ, 2012, P NATL ACAD SCI USA, V109, P12219, DOI 10.1073/pnas.1209954109; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zeng XQ, 2015, P NATL ACAD SCI USA, V112, P1095, DOI 10.1073/pnas.1423628112; Zhu SH, 2010, BBA-BIOENERGETICS, V1797, P1449, DOI 10.1016/j.bbabio.2010.04.003	98	9	9	10	16	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1874-7787	1876-7478		MAR GENOM	Mar. Genom.	AUG	2020	52								100740	10.1016/j.margen.2020.100740			11	Genetics & Heredity; Marine & Freshwater Biology	Genetics & Heredity; Marine & Freshwater Biology	MC4GL	WOS:000543247400007	31937506	Green Published, Green Accepted	Y	N	2021-04-07	
J	Baudry, L; Guiglielmoni, N; Marie-Nelly, H; Cormier, A; Marbouty, M; Avia, K; Mie, YL; Godfroy, O; Sterck, L; Cock, JM; Zimmer, C; Coelhe, SM; Koszul, R				Baudry, Lyam; Guiglielmoni, Nadege; Marie-Nelly, Herve; Cormier, Alexandre; Marbouty, Martial; Avia, Komlan; Mie, Yann Loe; Godfroy, Olivier; Sterck, Lieven; Cock, J. Mark; Zimmer, Christophe; Coelhe, Susana M.; Koszul, Romain			instaGRAAL: chromosome-level quality scaffolding of genomes using a proximity ligation-based scaffolder	GENOME BIOLOGY			English	Article						Ectocarpus; Hi-C scaffolding; Hi-C; genome assembly; MCMC; GPU; Desmarestia herbacea	ASSEMBLIES; ANNOTATION	Hi-C exploits contact frequencies between pairs of loci to bridge and order contigs during genome assembly, resulting in chromosome-level assemblies. Because few robust programs are available for this type of data, we developed instaGRAAL, a complete overhaul of the GRAAL program, which has adapted the latter to allow efficient assembly of large genomes. instaGRAAL features a number of improvements over GRAAL, including a modular correction approach that optionally integrates independent data. We validate the program using data for two brown algae, and human, to generate near-complete assemblies with minimal human intervention.	[Baudry, Lyam; Guiglielmoni, Nadege; Marie-Nelly, Herve; Marbouty, Martial; Koszul, Romain] Inst Pasteur, Unite Regulat Spatiale Genomes, CNRS, UMR 3525,C3BI,USR 3756, F-75015 Paris, France; [Baudry, Lyam; Marie-Nelly, Herve] Sorbonne Univ, Coll Doctoral, F-75005 Paris, France; [Guiglielmoni, Nadege] Univ Libre Bruxelles, Evolutionary Biol & Ecol, B-1050 Brussels, Belgium; [Cormier, Alexandre; Avia, Komlan; Godfroy, Olivier; Cock, J. Mark; Coelhe, Susana M.] Sorbonne Univ, Lab Integrat Biol Marine Models, Algal Genet, UMR 8227, Roscoff, France; [Avia, Komlan] Univ Strasbourg, INRA, SVQV UMR A 1131, Colmar, France; [Mie, Yann Loe] Inst Pasteur, Ctr Bioinformat Biostat & Integrat Biol C3BI, USR3756, CNRS, Paris, France; [Sterck, Lieven] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium; [Sterck, Lieven] VIB Ctr Plant Syst Biol, Technol Pk 927, B-9052 Ghent, Belgium; [Zimmer, Christophe] Inst Pasteur, Imaging & Modeling Unit, CNRS, UMR 3691,C3BI,USR 3756, F-75015 Paris, France	Koszul, R (corresponding author), Inst Pasteur, Unite Regulat Spatiale Genomes, CNRS, UMR 3525,C3BI,USR 3756, F-75015 Paris, France.; Coelhe, SM (corresponding author), Sorbonne Univ, Lab Integrat Biol Marine Models, Algal Genet, UMR 8227, Roscoff, France.	coelho@sbroscoff.fr; romain.koszul@pasteur.fr	Avia, Komlan/E-6850-2015	Avia, Komlan/0000-0001-6212-6774; Guiglielmoni, Nadege/0000-0002-6185-1592; Baudry, Lyam/0000-0002-9283-674X; Koszul, Romain/0000-0002-3086-1173; Cock, J. Mark/0000-0002-2650-0383	European Research Council under the Horizon 2020 Program (ERC) [260822, 638240]; European UnionEuropean Commission [764840]	This research was supported by funding to R.K. and S.M.C. from the European Research Council under the Horizon 2020 Program (ERC grant agreements 260822 and 638240, respectively). This project has also received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 764840.	Aganezov SS, 2017, BMC BIOINFORMATICS, V18, DOI 10.1186/s12859-017-1919-y; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Alhakami H, 2017, GENOME BIOL, V18, DOI 10.1186/s13059-017-1213-3; Alkan C, 2011, NAT REV GENET, V12, P363, DOI 10.1038/nrg2958; Antipov D, 2016, BIOINFORMATICS, V32, P1009, DOI 10.1093/bioinformatics/btv688; Appels R, 2018, SCIENCE, V361, P661, DOI 10.1126/science.aar7191; Arun A, 2019, ELIFE, V8, DOI 10.7554/eLife.43101; Avia K, 2017, SCI REP-UK, V7, DOI 10.1038/srep43241; Baudry L, 2020, SEQUENCE READ ARCH D; Bickhart DM, 2017, NAT GENET, V49, P643, DOI 10.1038/ng.3802; Bradnam KR, 2013, GIGASCIENCE, V2, DOI 10.1186/2047-217X-2-10; Burton JN, 2013, NAT BIOTECHNOL, V31, P1119, DOI 10.1038/nbt.2727; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Cournac A, 2012, BMC GENOMICS, V13, DOI 10.1186/1471-2164-13-436; Dekker J, 2002, SCIENCE, V295, P1306, DOI 10.1126/science.1067799; Dudchenko O, 2017, SCIENCE, V356, P92, DOI 10.1126/science.aal3327; English AK, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0041444; Flot JF, 2015, FEBS LETT, V589, P2966, DOI 10.1016/j.febslet.2015.04.034; Ghurye J, 2019, PLOS COMPUT BIOL, V15, DOI 10.1371/journal.pcbi.1007273; Wences AH, 2015, GENOME BIOL, V16, DOI 10.1186/s13059-015-0764-4; Imakaev M, 2012, NAT METHODS, V9, P999, DOI [10.1038/NMETH.2148, 10.1038/nmeth.2148]; Jourdier E, 2017, BIOTECHNOL BIOFUELS, V10, DOI 10.1186/s13068-017-0837-6; Kaplan N, 2013, NAT BIOTECHNOL, V31, P1143, DOI 10.1038/nbt.2768; Khan A, 2019, TANZ, P12; Kundu R, 2019, HYPO SUPER FAST ACCU; Lazar-Stefanita L, 2017, EMBO J, V36, P2684, DOI 10.15252/embj.201797342; Lieberman-Aiden E, 2009, SCIENCE, V326, P289, DOI 10.1126/science.1181369; Marbouty M, 2017, SCI ADV, V3, DOI 10.1126/sciadv.1602105; Marbouty M, 2014, ELIFE, V3, DOI 10.7554/eLife.03318; Marie-Nelly H, 2013, THESIS, P6; Marie-Nelly H, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms6695; Mikheenko A, 2018, BIOINFORMATICS, V34, P142, DOI 10.1093/bioinformatics/bty266; Miller JR, 2017, BMC GENOMICS, V18, DOI 10.1186/s12864-017-3927-8; MULLER DG, 1966, PLANTA, V68, P57, DOI 10.1007/BF00385371; Muller H, 2018, MOL SYST BIOL, V14, DOI 10.15252/msb.20188293; Putnam NH, 2016, GENOME RES, V26, P342, DOI 10.1101/gr.193474.115; RAMIREZ ME, 1986, CAN J BOT, V64, P2948, DOI 10.1139/b86-389; Rice ES, 2019, ANNU REV ANIM BIOSCI, V7, P17, DOI 10.1146/annurev-animal-020518-115344; Rippe K, 2001, TRENDS BIOCHEM SCI, V26, P733, DOI 10.1016/S0968-0004(01)01978-8; Salzberg SL, 2012, GENOME RES, V22, P557, DOI 10.1101/gr.131383.111; Sedlazeck FJ, 2018, NAT REV GENET, V19, P329, DOI 10.1038/s41576-018-0003-4; Simao FA, 2015, BIOINFORMATICS, V31, P3210, DOI 10.1093/bioinformatics/btv351; Vaser R, 2017, GENOME RES, V27, P737, DOI 10.1101/gr.214270.116; Walker BJ, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0112963; Yaffe E, 2011, NAT GENET, V43, P1059, DOI 10.1038/ng.947; Zimin AV, 2013, BIOINFORMATICS, V29, P2669, DOI 10.1093/bioinformatics/btt476	48	2	2	2	3	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1474-760X			GENOME BIOL	Genome Biol.	JUN 18	2020	21	1							148	10.1186/s13059-020-02041-z			22	Biotechnology & Applied Microbiology; Genetics & Heredity	Biotechnology & Applied Microbiology; Genetics & Heredity	MC0PI	WOS:000542999600001	32552806	DOAJ Gold, Green Published			2021-04-07	
J	Poza, AM; Santianez, WJE; Croce, ME; Gauna, MC; Kogame, K; Parodi, ER				Poza, Ailen M.; Santianez, Wilfred John E.; Croce, M. Emilia; Gauna, M. Cecilia; Kogame, Kazuhiro; Parodi, Elisa R.			Cryptic Haploid Stages in the Life Cycle ofLeathesia marina(Chordariaceae, Phaeophyceae) Under In Vitro Culture	JOURNAL OF PHYCOLOGY			English	Article						flow cytometry; fluorescent nuclei; microthalli; molecular identification; ploidy	ECTOCARPUS-SILICULOSUS ECTOCARPALES; LEATHESIA-DIFFORMIS; TEMPERATURE REQUIREMENTS; NUCLEAR-DNA; BROWN; ALGAE; EVOLUTION; MACROALGAE; HISTORIES; TOLERANCE	We evaluated the life cycle ofLeathesia marinathrough molecular analyses, culture studies, morphological observations, and ploidy measurements. Macroscopic sporophytes were collected from two localities in Atlantic Patagonia and were cultured under long-day (LD) and short-day (SD) conditions. Molecular identification of the microscopic and macroscopic phases was performed through thecox3 andrbcL genes and the phylogeny was assessed on the basis of single gene and concatenated datasets. Nuclear ploidy of each phase was estimated from the DNA contents of individual nuclei through epifluorescence microscopy and flow cytometry. Molecular results confirmed the identity of the Argentinian specimens asL. marinaand revealed their conspecificity withL. marinafrom New Zealand, Germany, and Japan. The sporophytic macrothalli (2n) released mitospores from plurilocular sporangia, which developed into globular microthalli (2n), morphologically similar to the sporophytes but not in size, constituting a generation of small diploid thalli, with a mean fluorescent nuclei cross-sectional area of 3.21 +/- 0.7 mu m(2). The unilocular sporangia released meiospores that developed two morphologically different types of microthalli: erect branched microthalli (n) with a nuclear area of 1.48 +/- 0.07 mu m(2)that reproduces asexually, and prostrate branched microthalli (n) with a nuclear area of 1.24 +/- 0.10 mu m(2)that reproduces sexually. The prostrate microthalli released gametes in LD conditions, which merged and produced macroscopic thalli with a nuclear cross-sectional area of 3.45 +/- 0.09 mu m(2). Flow cytometry confirmed that the erect and prostrate microthalli were haploid and that the globular microthalli and macrothalli were diploid.	[Poza, Ailen M.; Croce, M. Emilia; Gauna, M. Cecilia; Parodi, Elisa R.] CONICET Bahia Blanca, Inst Argentino Oceanog IADO, Camino Carrindanga 7-5 Km,B8000FWB, Bahia Blanca, Buenos Aires, Argentina; [Santianez, Wilfred John E.] Hokkaido Univ, Dept Nat Hist Sci, Grad Sch Sci, Sapporo, Hokkaido 0600810, Japan; [Santianez, Wilfred John E.] Univ Philippines Diliman, Inst Marine Sci, Coll Sci, Velasquez St, Quezon City 1101, Philippines; [Croce, M. Emilia; Gauna, M. Cecilia] Univ Nacl Sur, Lab Ecol Acuat Bot Marina & Acuicultura, Dept Biol Bioquim & Farm, San Juan 670,B8000FTN, Bahia Blanca, Buenos Aires, Argentina; [Kogame, Kazuhiro] Hokkaido Univ, Dept Biol Sci, Fac Sci, Sapporo, Hokkaido 0600810, Japan	Poza, AM (corresponding author), CONICET Bahia Blanca, Inst Argentino Oceanog IADO, Camino Carrindanga 7-5 Km,B8000FWB, Bahia Blanca, Buenos Aires, Argentina.	pozaailen@iado-conicet.gob.ar		Santianez, Wilfred John E./0000-0002-1994-4920	Secretaria General de Ciencia y Tecnologia, Universidad Nacional del Sur [PGI CSU-24/B234]; Consejo Nacional de Investigaciones Cientificas y TecnicasConsejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP-11220130100070CO]; Ministry of Education, Culture, Sports, Science and Technology (MEXT) of the Government of JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT); Department of Science and Technology (DOST) - Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD) of the Government of the Philippines	This work was funded by the Secretaria General de Ciencia y Tecnologia, Universidad Nacional del Sur under grant number PGI CSU-24/B234, and the Consejo Nacional de Investigaciones Cientificas y Tecnicas under grant number PIP-11220130100070CO. Collaborative work for this study was possible thanks to the Matsumae International Foundation (MIF) through the 2016 Research Fellowship Program. WJES is funded by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of the Government of Japan under the Monbukagakusho Scholarship Grant and the Department of Science and Technology (DOST) - Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD) of the Government of the Philippines through the DOST Balik Scientist Program.	ANDERSEN RA, 1992, BIODIVERS CONSERV, V1, P267, DOI 10.1007/BF00693765; Arun A., 2018, CONVERGENT RECRUITME; Arun A, 2019, ELIFE, V8, DOI 10.7554/eLife.43101; Bertness MD, 2006, ECOL MONOGR, V76, P439, DOI 10.1890/0012-9615(2006)076[0439:TCSOWA]2.0.CO;2; Bourdareau S., 2020, ECTOCARPUS; BRAWLEY SH, 1992, BRIT PHYCOL J, V27, P233, DOI 10.1080/00071619200650241; Carney Laura T., 2006, Algae, V21, P161; CHAPMAN A R O, 1971, Phycologia, V10, P63, DOI 10.2216/i0031-8884-10-1-63.1; CHAPMAN ARO, 1986, ADV MAR BIOL, V23, P1; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; CHOI HG, 1994, KOREAN J PHYCOLOGY, V9, P21; CLAYTON MN, 1988, BOT MAR, V31, P379, DOI 10.1515/botm.1988.31.5.379; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; COLE K, 1968, CAN J GENET CYTOL, V10, P63, DOI 10.1139/g68-009; Cole K. M., 1990, BIOL RED ALGAE, P73; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; CROW JAMES F., 1965, AMER NATUR, V99, P439, DOI 10.1086/282389; CUNNINGHAM EM, 1993, J EXP MAR BIOL ECOL, V171, P1, DOI 10.1016/0022-0981(93)90136-C; DAMMANN HILDEGARD, 1930, WISS MEERESUNTERSUCH ABT HEL GOLAND, V18, P1; Dangeard P., 1969, PHEOSPOREES BOT, V52, P59; Dangeard P., 1965, BOTANISTE, V48, P5; Edwards MS, 2000, ECOLOGY, V81, P2404, DOI 10.2307/177463; Heesch S., 2019, EVOLUTION LIFE CYCLE; HOFFMANN AJ, 1988, J PHYCOL, V24, P203; HOFFMANN AJ, 1991, MAR ECOL PROG SER, V79, P185, DOI 10.3354/meps079185; Hothorn T, 2008, BIOMETRICAL J, V50, P346, DOI 10.1002/bimj.200810425; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; Hurd CL, 2014, SEAWEED ECOLOGY AND PHYSIOLOGY, 2ND EDITION, P1, DOI 10.1017/CBO9781139192637; Hwang IK, 2005, MAR BIOL, V147, P999, DOI 10.1007/s00227-005-1623-8; KAPRAUN DF, 1994, PHYCOLOGIA, V33, P42, DOI 10.2216/i0031-8884-33-1-42.1; KLINGER T, 1993, TRENDS ECOL EVOL, V8, P256, DOI 10.1016/0169-5347(93)90202-Z; Kylin H., 1933, LUNDS U ARSSKR, V29, P1; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Gall L, 2010, J PHYCOL, V46, P374, DOI 10.1111/j.1529-8817.2010.00807.x; LEGALL Y, 1993, PROTOPLASMA, V173, P123; LUBCHENCO J, 1980, ECOLOGY, V61, P676, DOI 10.2307/1937433; Mable BK, 1998, BIOESSAYS, V20, P453, DOI 10.1002/(SICI)1521-1878(199806)20:6<453::AID-BIES3>3.0.CO;2-N; Mair P, 2020, BEHAV RES METHODS, V52, P464, DOI 10.3758/s13428-019-01246-w; Mann K. H., 1982, ECOLOGY COASTAL WATE; Miller MA., 2010, CREATING CIPRES SCI, DOI [DOI 10.1109/GCE.2010.5676129, 10.1109/GCE.2010.5676129]; Muller D. G., 1980, PUBL WISS FILMEN SEK, V13 11/C 1308, P1; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Nakahara H., 1984, SCI PAPERS I ALGOLOG, V7, P91; NYGREN S, 1975, BOT MAR, V18, P131, DOI 10.1515/botm.1975.18.3.131; OATES BR, 1989, BOT MAR, V32, P475, DOI 10.1515/botm.1989.32.5.475; Otto SP, 1996, BIOL J LINN SOC, V57, P197, DOI 10.1111/j.1095-8312.1996.tb00309.x; Paruelo Jose M., 1998, Ecologia Austral, V8, P85; Peters A.F., 1987, PROGR PHYCOLOGICAL R, V5, P223; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Phillips N, 2008, J PHYCOL, V44, P394, DOI 10.1111/j.1529-8817.2008.00473.x; Poza AM, 2017, PHYCOLOGIA, V56, P579, DOI 10.2216/16-117.1; PRICE HJ, 1976, BOT REV, V42, P27, DOI 10.1007/BF02860861; Provasoli L, 1968, CULTURES COLLECTIONS, P63; Quartino M. L., 1996, Revista Brasileira de Biologia, V56, P139; R CoreTeam, 2016, R LANG ENV STAT COMP; RUSSELL G, 1986, OCEANOGR MAR BIOL, V24, P309; Santianez WJE, 2018, PHYCOLOGIA, V57, P61, DOI 10.2216/17-68.1; Sauvageau C, 1925, CR HEBD ACAD SCI, V180, P1632; Schiel DR, 2006, ANNU REV ECOL EVOL S, V37, P343, DOI 10.1146/annurev.ecolsys.37.091305.110251; SPARROW AH, 1961, SCIENCE, V134, P282, DOI 10.1126/science.134.3474.282; Stamatakis A, 2014, BIOINFORMATICS, V30, P1312, DOI 10.1093/bioinformatics/btu033; Swanson AK, 2000, MAR BIOL, V136, P657, DOI 10.1007/s002270050725; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; WEBBER EE, 1981, BOT MAR, V24, P297; WHITTICK A, 1987, BRIT PHYCOL J, V22, P314; WIENCKE C, 1990, MAR ECOL PROG SER, V59, P157, DOI 10.3354/meps059157; WIENCKE C, 1989, MAR ECOL PROG SER, V54, P189, DOI 10.3354/meps054189; ZUPAN JR, 1990, J PHYCOL, V26, P232, DOI 10.1111/j.0022-3646.1990.00232.x	70	0	0	0	2	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	OCT	2020	56	5					1349	1361		10.1111/jpy.13034		JUN 2020	13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	OE7VV	WOS:000540197500001	32463924				2021-04-07	
J	Carrano, MW; Yarimizu, K; Gonzales, JL; Cruz-Lopez, R; Edwards, MS; Tymon, TM; Kupper, FC; Carrano, CJ				Carrano, Mary W.; Yarimizu, Kyoko; Gonzales, Jennifer L.; Cruz-Lopez, Ricardo; Edwards, Matthew S.; Tymon, Teresa M.; Kupper, Frithjof C.; Carrano, Carl J.			The influence of marine algae on iodine speciation in the coastal ocean	ALGAE			English	Article						Ectocarpus; iodate; iodide; iodine speciation; Lingulodinium; Macrocystis; phytoplankton	DISSOLVED IODATE; PHYTOPLANKTON; SEAWATER; STORAGE; TRANSFORMATION; OXIDATION; ATLANTIC; KELP; SEA	Iodine exists as a trace element in seawater, with total iodine being generally constant at about 0.45-0.55 mu M. Almost all of this iodine occurs in two main forms: iodate and iodide. Iodate is the thermodynamically stable form under normal seawater conditions, and thus should be the only iodine-containing species in the water column. However, iodate concentrations are found to vary considerably, being generally greater at depth and lower at the surface, while iodide concentrations follow the reverse pattern, being anomalously accumulated in the euphotic zone and decreasing with depth. The fact that iodide concentrations follow a depth dependence corresponding to the euphotic zone suggests that biological activity is the source of the reduced iodine. Nonetheless, the nature and source of iodate reduction activity remains controversial. Here, using a combination of field and laboratory studies, we examine some of the questions raised in our and other previous studies, and seek further correlations between changes in iodine speciation and the presence of marine macro- and microalgae. The present results indicate that microalgal growth per se does not seem to be responsible for the reduction of iodate to iodide. However, there is some support for the hypothesis that iodate reduction can occur due to release of cellular reducing agents that accompany cell senescence during phytoplankton bloom declines. In addition, support is given to the concept that macroalgal species such as giant kelp (Macrocystis pyrifera) can take up both iodide and iodate from seawater (albeit on a slower time scale). We propose a mechanism whereby iodate is reduced to iodide at the cell surface by cell surface reductases and is taken up directly as such without reentering the bulk solution.	[Carrano, Mary W.; Yarimizu, Kyoko; Gonzales, Jennifer L.; Cruz-Lopez, Ricardo; Tymon, Teresa M.; Carrano, Carl J.] San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA; [Edwards, Matthew S.] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA; [Kupper, Frithjof C.] Univ Aberdeen, Sch Biol Sci, Cruickshank Bldg,St Machar Dr, Aberdeen AB24 3UU, Scotland	Carrano, CJ (corresponding author), San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA.	ccarrano@mail.sdsu.edu		Yarimizu, Kyoko/0000-0001-7492-6592	National Science FoundationNational Science Foundation (NSF) [CHE-1664657]; TOTAL Foundation (Paris); UK Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/D521522/1, NE/J023094/1, Oceans 2025/WP 4.5]; MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland); Hanse-Wissenschaftskolleg	This work was supported in part by grant CHE-1664657 from the National Science Foundation to CJC and FCK, the TOTAL Foundation (Paris) and the UK Natural Environment Research Council grants (NE/D521522/1, NE/J023094/1, Oceans 2025/WP 4.5) to FCK. We are also grateful for funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). We thank Dr. M. L. Carter, SIO for help with collection of water samples at Scripps Pier, Cesar O. Almeda-Jauregui, CICESE for Ocean Data View plots and Dr. Avery Tatters, USC for the initial culture of Lingulodinium polyedra. A fellowship from the Hanse-Wissenschaftskolleg to CJC is also gratefully acknowledged.	Bluhm K, 2010, AQUAT BIOL, V11, P1, DOI 10.3354/ab00284; Bottger LH, 2012, J EXP BOT, V63, P5763, DOI 10.1093/jxb/ers225; BUTLER ECV, 1981, LIMNOL OCEANOGR, V26, P382, DOI 10.4319/lo.1981.26.2.0382; Campos MLAM, 1996, DEEP-SEA RES PT II, V43, P455, DOI 10.1016/0967-0645(95)00100-X; Campos MLAM, 1997, MAR CHEM, V57, P107, DOI 10.1016/S0304-4203(96)00093-X; Campos MLAM, 1999, MAR CHEM, V65, P167, DOI 10.1016/S0304-4203(98)00094-2; Carpenter LJ, 2003, CHEM REV, V103, P4953, DOI 10.1021/cr0206465; Chance R, 2014, ENVIRON SCI-PROC IMP, V16, P1841, DOI 10.1039/c4em00139g; Chance R, 2009, ESTUAR COAST SHELF S, V82, P406, DOI 10.1016/j.ecss.2009.02.004; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Davis D, 1996, J GEOPHYS RES-ATMOS, V101, P2135, DOI 10.1029/95JD02727; Farrenkopf AM, 1997, MAR CHEM, V57, P347, DOI 10.1016/S0304-4203(97)00039-X; Gonzales J, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0180755; Hardisty DS, 2014, GEOLOGY, V42, P619, DOI 10.1130/G35439.1; HERRING JR, 1974, DEEP-SEA RES, V21, P777, DOI 10.1016/0011-7471(74)90085-0; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kupper FC, 1998, PLANTA, V207, P163, DOI 10.1007/s004250050469; Kupper FC, 2019, METALLOMICS, V11, P756, DOI [10.1039/c8mt00327k, 10.1039/C8MT00327K]; Kupper FC, 2018, J BIOL INORG CHEM, V23, P1119, DOI 10.1007/s00775-018-1539-7; LUTHER GW, 1995, ADV CHEM SER, V244, P135, DOI 10.1021/ba-1995-0244.ch006; LUTHER GW, 1988, ANAL CHEM, V60, P1721, DOI 10.1021/ac00168a017; Moorthi SD, 2006, MICROB ECOL, V52, P136, DOI 10.1007/s00248-006-9030-3; O'Dowd CD, 2002, NATURE, V417, P632, DOI 10.1038/nature00775; Quack B, 2004, GEOPHYS RES LETT, V31, DOI 10.1029/2004GL020597; Saiz-Lopez A, 2006, ATMOS CHEM PHYS, V6, P883, DOI 10.5194/acp-6-883-2006; Saiz-Lopez A, 2012, CHEM REV, V112, P1773, DOI 10.1021/cr200029u; Salawitch RJ, 2005, GEOPHYS RES LETT, V32, DOI 10.1029/2004GL021504; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; TAKAYANAGI K, 1986, TALANTA, V33, P451, DOI 10.1016/0039-9140(86)80115-1; Truesdale VW, 2008, ESTUAR COAST SHELF S, V78, P155, DOI 10.1016/j.ecss.2007.11.022; TRUESDALE VW, 1978, MAR CHEM, V6, P253, DOI 10.1016/0304-4203(78)90034-8; Truesdale VW, 2003, LIMNOL OCEANOGR, V48, P1569, DOI 10.4319/lo.2003.48.4.1569; Tymon TM, 2017, J INORG BIOCHEM, V177, P82, DOI 10.1016/j.jinorgbio.2017.09.003; Wong GTF, 2002, MAR ECOL PROG SER, V237, P27, DOI 10.3354/meps237027; Yarimizu K, 2019, BIOMETALS, V32, P139, DOI 10.1007/s10534-018-00163-3; Yarimizu K, 2017, BIOMETALS, V30, P945, DOI 10.1007/s10534-017-0061-7	36	0	0	3	7	KOREAN SOC PHYCOLOGY	SEOUL	B1F, TRUST TOWER, 275-7 YANGJAE-DONG, SEOCHO-KU, SEOUL, 137-739, SOUTH KOREA	1226-2617	2093-0860		ALGAE-SEOUL	Algae	JUN	2020	35	2					167	176		10.4490/algae.2020.35.5.25			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	MB5HI	WOS:000542633200006		Other Gold			2021-04-07	
J	Li, JY; Zhang, XC; Li, D; Sun, MY; Shi, L				Li, Jing-Yi; Zhang, Xian-Chun; Li, Dong; Sun, Mei-Yu; Shi, Lei			Energy response patterns to light spectrum at sex differentiation stages of Drynaria roosii gametophytes	ENVIRONMENTAL AND EXPERIMENTAL BOTANY			English	Article						Drynaria roosii gametophyte; Energy response; Light spectrum; Sex differentiation; WGCNA	INORGANIC CARBON ACQUISITION; BLUE-LIGHT; ECTOCARPUS-SILICULOSUS; GENE-EXPRESSION; PHOTOSYSTEM-I; RED-LIGHT; PHOTOSYNTHESIS; QUALITY; ARABIDOPSIS; ALLOCATION	The environmental light changing, such as light spectrum (LS), influences the balance of energy metabolism and sex allocation based on plant species. However, the molecular mechanism underlying systems energy responses (SERs) linked to sex differentiation of Drynaria roosii gametophytes in response to IS remains the enigma. Here, we used Red-LED-Light (RLL), Blue-LED-Light (BLL) and Fluorescent-Light (FL) regimes to treat D. roosii gametophytes to achieve the knowledge of global expression database. Compared with FL, transcriptional changes showed that RLL reduced mRNA levels of photosynthetic genes and improved mRNA levels of respiratory genes. Obviously, RLL decreased PSII/PSI activities with lower Y(II), Fv/Fm, Y(I) and ETR(I) values, and promoted mitochondria activity with higher respiratory rate. Combined with severer ROS stress, we suggested that RLL caused the energy deficit to gametophytes. However, the impacts of BLL were contrary to RLL. Exactly, 63.75 % of males was induced under RLL vs 12.70 % under FL, whilst 64.50 % of females under BLL vs 48.08 % under FL. Moreover, we applied the WGCNA to cluster IS-induced DEGs into eleven modules, and DEGs in five significant sample-specific modules mainly fell in energy metabolism categorization through KEGG pathway analysis. Networks proved the complicated 'cross-talk' between SER- and TF-associated genes, and revealed hub genes in response to LS. Based on previous experimental evidence, we supposed a link between LS-induced energy metabolism responses and sex differentiation in D. roosii gametophytes, showing that RLL contributed to malebiased sex ratios for less energy production than BLL and FL, while BLL contributed to a female-biased trend. Our work firstly shed light on SERs to LS in D. roosii gametophytes, and provided new insights for understanding the sex differentiation in ferns.	[Li, Jing-Yi; Li, Dong; Sun, Mei-Yu; Shi, Lei] Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China; [Zhang, Xian-Chun] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China	Sun, MY; Shi, L (corresponding author), Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China.	jingyileesd@126.com; zhangxc@ibcas.ac.cn; lidongfern@126.com; sunmeiyu@ibcas.ac.cn; shilei_67@126.com			National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81573519]; Key Projects of the Chinese Academy of Sciences [KZCC-EW-103-3]	This work was financially supported by the National Natural Science Foundation of China [grant no. 81573519] and the Key Projects of the Chinese Academy of Sciences [grant no. KZCC-EW-103-3].	Aguilera J, 2000, J PLANT PHYSIOL, V157, P86, DOI 10.1016/S0176-1617(00)80140-6; ANDERS S, 2010, GENOME BIOL, V11, DOI DOI 10.1186/GB-2010-11-10-R106; ARNON DI, 1949, PLANT PHYSIOL, V24, P1, DOI 10.1104/pp.24.1.1; Asada K, 2006, PLANT PHYSIOL, V141, P391, DOI 10.1104/pp.106.082040; Banks JA, 1997, TRENDS PLANT SCI, V2, P175, DOI 10.1016/S1360-1385(97)85223-5; Deng XG, 2015, J EXP BOT, V66, P6219, DOI 10.1093/jxb/erv328; DeSoto L, 2008, J ECOL, V96, P1319, DOI 10.1111/j.1365-2745.2008.01425.x; Dinakar C, 2010, PLANTA, V231, P461, DOI 10.1007/s00425-009-1067-3; Dong Y, 2018, MOL BIOL EVOL, V35, P1901, DOI 10.1093/molbev/msy090; Field DL, 2013, ANN BOT-LONDON, V111, P917, DOI 10.1093/aob/mct040; Flexas J., 2005, PLANT RESP CELL ECOS, P85; Flores-Renteria L, 2013, AM J BOT, V100, P602, DOI 10.3732/ajb.1200068; Foyer CH, 2011, PLANT PHYSIOL, V155, P93, DOI 10.1104/pp.110.166181; Gerttula S, 2015, PLANT CELL, V27, P2800, DOI 10.1105/tpc.15.00531; GOLDMAN DA, 1986, BOT REV, V52, P157, DOI 10.1007/BF02861000; Guillon JM, 2003, J ECOL, V91, P49, DOI 10.1046/j.1365-2745.2003.00744.x; HAUKE RL, 1971, AM J BOT, V58, P373, DOI 10.2307/2441127; HERMSMEIER D, 1991, J PHOTOCH PHOTOBIO B, V11, P189, DOI 10.1016/1011-1344(91)80260-O; HICKMAN JC, 1975, OECOLOGIA, V21, P117, DOI 10.1007/BF00345554; Hillrichs S, 2001, EUR J PHYCOL, V36, P71, DOI 10.1017/S096702620100302X; Hogewoning SW, 2010, J EXP BOT, V61, P3107, DOI 10.1093/jxb/erq132; Huang JY, 2018, J SCI FOOD AGR, V98, P5486, DOI 10.1002/jsfa.9093; Igamberdiev AU, 2014, PLANT CELL ENVIRON, V37, P290, DOI 10.1111/pce.12155; Jesson LK, 2012, PERSPECT PLANT ECOL, V14, P153, DOI 10.1016/j.ppees.2011.10.003; Jungandreas A, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0099727; Kamachi H, 2007, J PLANT RES, V120, P629, DOI 10.1007/s10265-007-0106-z; Kangasjarvi S, 2012, J EXP BOT, V63, P1619, DOI 10.1093/jxb/err402; Kasajima SY, 2008, PLANT PROD SCI, V11, P76, DOI 10.1626/pps.11.76; Langfelder P, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-559; Li JY, 2018, ENVIRON EXP BOT, V156, P298, DOI 10.1016/j.envexpbot.2018.09.017; Li JY, 2015, PLANT GROWTH REGUL, V77, P33, DOI 10.1007/s10725-015-0032-3; Liao JC, 2016, PHOTOSYNTH RES, V127, P151, DOI 10.1007/s11120-015-0161-6; Lima JMT, 2018, PLANT ECOL, V219, P1225, DOI 10.1007/s11258-018-0874-7; Lin CW, 2019, BMC PLANT BIOL, V19, DOI 10.1186/s12870-018-1613-x; Liu XY, 2011, HORTSCIENCE, V46, P217, DOI 10.21273/HORTSCI.46.2.217; LLOYD DG, 1984, EVOL BIOL, V17, P255; LOKESHA R, 1993, CURR SCI INDIA, V65, P238; Lv JY, 2017, FOOD CHEM, V216, P225, DOI 10.1016/j.foodchem.2016.08.005; Mastropasqua L, 2012, PLANT SCI, V183, P57, DOI 10.1016/j.plantsci.2011.11.009; Millar AH, 2011, ANNU REV PLANT BIOL, V62, P79, DOI 10.1146/annurev-arplant-042110-103857; Mochizuki T, 2004, FEBS LETT, V571, P26, DOI 10.1016/j.febslet.2004.06.052; Moeder W, 2002, PLANT PHYSIOL, V130, P1918, DOI 10.1104/pp.009712; Munekage Y, 2002, CELL, V110, P361, DOI 10.1016/S0092-8674(02)00867-X; Nascimento LBS, 2013, PHOTOCHEM PHOTOBIOL, V89, P391, DOI 10.1111/php.12006; Norman C, 2004, PLANT PHYSIOL, V134, P492, DOI 10.1104/pp.103.031039; O'Leary BM, 2020, PLANT CELL, V32, P666, DOI 10.1105/tpc.19.00157; Overmyer K, 2003, TRENDS PLANT SCI, V8, P335, DOI 10.1016/S1360-1385(03)00135-3; Pallozzi E, 2013, ENVIRON EXP BOT, V95, P50, DOI 10.1016/j.envexpbot.2013.06.001; Pinson JB, 2017, INT J PLANT SCI, V178, P1, DOI 10.1086/688773; Pribil M, 2014, J EXP BOT, V65, P1955, DOI 10.1093/jxb/eru090; Pu XJ, 2015, ANN BOT-LONDON, V116, P583, DOI 10.1093/aob/mcv063; Raghavan V, 1989, DEV BIOL FERN GAMETO; RAM HYM, 1970, PHYTOMORPHOLOGY, V20, P151; Sacco A, 2019, BMC GENOMICS, V20, DOI 10.1186/s12864-019-5428-4; Sanderson BJ, 2019, NEW PHYTOL, V221, P527, DOI 10.1111/nph.15421; Schenk PM, 2000, P NATL ACAD SCI USA, V97, P11655, DOI 10.1073/pnas.97.21.11655; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; Sewelam N, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0070289; SOLOMON BP, 1985, ECOLOGY, V66, P1321, DOI 10.2307/1939185; Su NN, 2014, PLANT GROWTH REGUL, V73, P227, DOI 10.1007/s10725-013-9883-7; Suetsugu N, 2003, CURR OPIN PLANT BIOL, V6, P91, DOI 10.1016/S1369526602000067; Sun MY, 2018, PLANT CELL PHYSIOL, V59, P1398, DOI 10.1093/pcp/pcy072; Sun W, 2012, PLANT CELL ENVIRON, V35, P982, DOI 10.1111/j.1365-3040.2011.02466.x; Suzuki N, 2012, PLANT CELL ENVIRON, V35, P259, DOI 10.1111/j.1365-3040.2011.02336.x; Szechynska-Hebda M, 2013, J PLANT PHYSIOL, V170, P1501, DOI 10.1016/j.jplph.2013.06.005; Takagi E, 2012, BOTANY, V90, P75, DOI [10.1139/B11-085, 10.1139/b11-085]; Vega-Frutis R, 2014, J EVOLUTION BIOL, V27, P667, DOI 10.1111/jeb.12333; Wada M, 1997, PLANT CELL ENVIRON, V20, P685, DOI 10.1046/j.1365-3040.1997.d01-118.x; Wang H, 2010, EUR J PLANT PATHOL, V127, P125, DOI 10.1007/s10658-009-9577-1; Wang H, 2009, J PHOTOCH PHOTOBIO B, V96, P30, DOI 10.1016/j.jphotobiol.2009.03.010; Wang L, 2017, MOL PLANT, V10, P183, DOI 10.1016/j.molp.2016.12.006; Wang P, 2017, NEW PHYTOL, V216, P32, DOI 10.1111/nph.14682; Xu F, 2014, SCI HORTIC-AMSTERDAM, V175, P181, DOI 10.1016/j.scienta.2014.06.012; Xu X, 2008, PLANT CELL ENVIRON, V31, P850, DOI 10.1111/j.1365-3040.2008.01799.x; [杨斌 Yang Bin], 2010, [中国野生植物资源, Chinese Wild Plant Resources], V29, P1; YUNGHANS H, 1972, PLANT PHYSIOL, V49, P1, DOI 10.1104/pp.49.1.1; Zheng L, 2017, FRONT PLANT SCI, V8, DOI 10.3389/fpls.2017.00917; ZIMMERMAN JK, 1991, ECOLOGY, V72, P597, DOI 10.2307/2937200	78	0	0	3	5	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND	0098-8472	1873-7307		ENVIRON EXP BOT	Environ. Exp. Bot.	APR	2020	172								103996	10.1016/j.envexpbot.2020.103996			16	Plant Sciences; Environmental Sciences	Plant Sciences; Environmental Sciences & Ecology	KT0MG	WOS:000518704100006		Other Gold			2021-04-07	
J	Dittami, SM; Peters, AF; West, JA; Cariou, T; KleinJan, H; Burgunter-Delamare, B; Prechoux, A; Egan, S; Boyen, C				Dittami, Simon M.; Peters, Akira F.; West, John A.; Cariou, Thierry; KleinJan, Hetty; Burgunter-Delamare, Bertille; Prechoux, Aurelie; Egan, Suhelen; Boyen, Catherine			Revisiting Australian Ectocarpus subulatus (Phaeophyceae) From the Hopkins River: Distribution, Abiotic Environment, and Associated Microbiota	JOURNAL OF PHYCOLOGY			English	Article						distribution; Ectocarpus subulatus; freshwater colonization; low salinity adaptation; microbiota	MARINE; ALGAE; DIVERSITY; EVOLUTION	In 1995 a strain of Ectocarpus was isolated from Hopkins River Falls, Victoria, Australia, constituting one of few available freshwater or nearly freshwater brown algae, and the only one belonging to the genus Ectocarpus. It has since been used as a model to study acclimation and adaptation to low salinities and the role of its microbiota in these processes. To provide more background information on this model, we assessed if Ectocarpus was still present in the Hopkins river 22 years after the original finding, estimated its present distribution, described its abiotic environment, and determined its in situ microbial composition. We sampled for Ectocarpus at 15 sites along the Hopkins River as well as 10 neighboring sites and found individuals with ITS and cox1 sequences identical to the original isolate at three sites upstream of Hopkins River Falls. The salinity of the water at these sites ranged from 3.1 to 6.9, and it was rich in sulfate (1-5 mM). The diversity of bacteria associated with the algae in situ (1312 operational taxonomic units) was one order of magnitude higher than in previous studies of the original laboratory culture, and 95 alga-associated bacterial strains were isolated from algal filaments on site. In particular, species of Planctomycetes were abundant in situ but rare in laboratory cultures. Our results confirmed that Ectocarpus was still present in the Hopkins River, and the newly isolated algal and bacterial strains offer new possibilities to study the adaptation of Ectocarpus to low salinity and its interactions with its microbiome.	[Dittami, Simon M.; KleinJan, Hetty; Burgunter-Delamare, Bertille; Prechoux, Aurelie; Boyen, Catherine] Sorbonne Univ, Stn Biol Roscoff, Integrat Biol Marine Models LBI2M, CNRS, F-29680 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, 40 Rue Pecheurs, F-29250 Santec, France; [West, John A.] Univ Melbourne, Biosci 2, Parkville, Vic 3010, Australia; [Cariou, Thierry] Sorbonne Univ, Stn Biol Roscoff, CNRS, FR2424, F-29680 Roscoff, France; [Egan, Suhelen] Univ New South Wales, Sch Biol Earth & Environm Sci, Ctr Marine Sci & Innovat, Sydney, NSW, Australia; [KleinJan, Hetty] Res & Expertise Ctr Water, CEBEDEAU, Allee Decouverte,11 B53,Quartier Polytech 1, B-4000 Liege, Belgium; [Prechoux, Aurelie] ALGAIA, Res & Dev Ctr, 91 Rue Edouard Branly, F-50000 St Lo, France	Dittami, SM (corresponding author), Sorbonne Univ, Stn Biol Roscoff, Integrat Biol Marine Models LBI2M, CNRS, F-29680 Roscoff, France.	simon.dittami@sb-roscoff.fr	Dittami, Simon/E-8354-2011	Dittami, Simon/0000-0001-7987-7523; Peters, Akira/0000-0001-5332-199X; Egan, Suhelen/0000-0003-3286-4279	ANR project IDEALGFrench National Research Agency (ANR) [ANR-10-BTBR-04]; European Union's Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie grant [624575]; CNRS, Sorbonne University [UMR8227]	This work was funded partially by ANR project IDEALG (ANR-10-BTBR-04) "Investissements d'Avenir, Biotechnologies-Bioressources", the European Union's Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie grant agreement number 624575 (ALFF), and an internal call for proposals from the UMR8227 (CNRS, Sorbonne University). We thank Cecile Herve, Amandine Simeon, and Agnieszka P. Lipinska for helpful discussions; Gwenn Tanguy and Erwan Legeay from the GENOMER platform; Roscoff for support during the library construction and sequencing; and the ABIMS platform for providing the computational facilities for the amplicon sequencing analyses.	Aminot A, 2007, DOSAGE AUTOMATIQUE N; [Anonymous], 1958, LIMNOL OCEANOGR, V3, P346; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Dittami S.M., 2019, PEERJ PREPR, V7, DOI DOI 10.7287/PEERJ.PREPRINTS.27519V3; Dittami SM, 2020, MAR GENOM, V52, DOI 10.1016/j.margen.2020.100740; Dittami SM, 2017, J PHYCOL, V53, P731, DOI 10.1111/jpy.12547; Dittami SM, 2016, ISME J, V10, P51, DOI 10.1038/ismej.2015.104; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; FOFONOFF NP, 1983, UNESCO TECHNICAL PAP, P44; GEISSLER U, 1983, NOVA HEDWIGIA, V37, P193; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; KleinJan H, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.02456; Kozich JJ, 2013, APPL ENVIRON MICROB, V79, P5112, DOI 10.1128/AEM.01043-13; Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]; Lage OM, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00405; Lane CE, 2007, MOL PHYLOGENET EVOL, V44, P634, DOI 10.1016/j.ympev.2007.03.016; Lundholm N, 2003, J PHYCOL, V39, P797, DOI 10.1046/j.1529-8817.2003.02031.x; Masella AP, 2012, BMC BIOINFORMATICS, V13, DOI 10.1186/1471-2105-13-31; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2015, CRYPTOGAMIE ALGOL, V36, P3, DOI 10.7872/crya.v36.iss1.2015.3; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Prechoux A, 2016, MAR BIOTECHNOL, V18, P133, DOI 10.1007/s10126-015-9675-3; Rognes T, 2016, PEERJ, V4, DOI 10.7717/peerj.2584; STACKEBRANDT E, 1994, INT J SYST BACTERIOL, V44, P846, DOI 10.1099/00207713-44-4-846; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Tapia JE, 2016, FRONT MICROBIOL, V7, DOI [10.3389/fmicb.2016.00197, 10.3389/fmicb.2016.00107]; Thomas F, 2020, ENV MICROBIOL REP, V12, P30, DOI 10.1111/1758-2229.12806; Torode TA, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0118366; Wang Q, 2007, APPL ENVIRON MICROB, V73, P5261, DOI 10.1128/AEM.00062-07; WEISBURG WG, 1991, J BACTERIOL, V173, P697, DOI 10.1128/JB.173.2.697-703.1991; West John A., 1996, Muelleria, V9, P29; Wetzel, 2001, LIMNOLOGY LAKE RIVER; Zobell CE, 1941, J MAR RES, V4, P42; Zuljevic A, 2016, SCI REP-UK, V6, DOI 10.1038/srep19642	37	2	2	2	4	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	JUN	2020	56	3					719	729		10.1111/jpy.12970		FEB 2020	11	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	LX4ST	WOS:000516815700001	31965565				2021-04-07	
J	Burgunter-Delamare, B; KleinJan, H; Frioux, C; Fremy, E; Wagner, M; Corre, E; Le Salver, A; Leroux, C; Leblanc, C; Boyen, C; Siegel, A; Dittami, SM				Burgunter-Delamare, Bertille; KleinJan, Hetty; Frioux, Clemence; Fremy, Enora; Wagner, Margot; Corre, Erwan; Le Salver, Alicia; Leroux, Cedric; Leblanc, Catherine; Boyen, Catherine; Siegel, Anne; Dittami, Simon M.			Metabolic Complementarity Between a Brown Alga and Associated Cultivable Bacteria Provide Indications of Beneficial Interactions	FRONTIERS IN MARINE SCIENCE			English	Article						Ectocarpus siliculosus; symbiotic/mutualistic bacteria; genome-scale metabolic networks; metabolic complementarity; holobiont	NETWORK-BASED TOOL	Brown algae are key components of marine ecosystems and live in association with bacteria that are essential for their growth and development. Ectocarpus siliculosus is a genetic and genomic model for brown algae. Here we use this model to start disentangling the complex interactions that may occur between the algal host and its associated bacteria. We report the genome-sequencing of 10 alga-associated bacteria and the genome-based reconstruction of their metabolic networks. The predicted metabolic capacities were then used to identify metabolic complementarities between the algal host and the bacteria, highlighting a range of potentially beneficial metabolite exchanges between them. These putative exchanges allowed us to predict consortia consisting of a subset of these ten bacteria that would best complement the algal metabolism. Finally, co-culture experiments were set up with a subset of these consortia to monitor algal growth as well as the presence of key algal metabolites. Although we did not fully control but only modified bacterial communities in our experiments, our data demonstrated a significant increase in algal growth in cultures inoculated with the selected consortia. In several cases, we also detected, in algal extracts, the presence of key metabolites predicted to become producible via an exchange of metabolites between the alga and the microbiome. Thus, although further methodological developments will be necessary to better control and understand microbial interactions in Ectocarpus, our data suggest that metabolic complementarity is a good indicator of beneficial metabolite exchanges in the holobiont.	[Burgunter-Delamare, Bertille; KleinJan, Hetty; Leblanc, Catherine; Boyen, Catherine; Dittami, Simon M.] Sorbonne Univ, Integrat Biol Marine Models LBI2M, CNRS, Roscoff, France; [Frioux, Clemence; Fremy, Enora; Wagner, Margot; Siegel, Anne] Univ Rennes, IRISA, CNRS, INRIA, Rennes, France; [Corre, Erwan; Le Salver, Alicia; Leroux, Cedric] Sorbonne Univ, Stn Biol Roscoff, FR2424, CNRS, Roscoff, France	Dittami, SM (corresponding author), Sorbonne Univ, Integrat Biol Marine Models LBI2M, CNRS, Roscoff, France.	simon.dittami@sb-roscoff.fr	Frioux, Clemence/A-1517-2019; corre, erwan/O-4669-2019	Frioux, Clemence/0000-0003-2114-0697; corre, erwan/0000-0001-6354-2278; LEROUX, Cedric/0000-0001-9225-1234	CNRS Momentum call; ANR project IDEALGFrench National Research Agency (ANR) [ANR-10-BTBR-04]; European UnionEuropean Commission [624575]; Brittany region (Project HOSALA); Sorbonne University [ED227]	This work was funded partially by the CNRS Momentum call, the ANR project IDEALG (ANR-10-BTBR-04) "Investissements d'Avenir, Biotechnologies-Bioressources," the European Union's Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie grant agreement number 624575 (ALFF), and joint Ph.D. scholarship from the Brittany region (Project HOSALA) and the Sorbonne University (ED227).	Aite M, 2018, PLOS COMPUT BIOL, V14, DOI 10.1371/journal.pcbi.1006146; Amin SA, 2015, NATURE, V522, P98, DOI 10.1038/nature14488; Aziz RK, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-75; Bankevich A, 2012, J COMPUT BIOL, V19, P455, DOI 10.1089/cmb.2012.0021; BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Burgunter-Delamare B, 2019, PREPRINT, DOI [10.1101/813683, DOI 10.1101/813683]; de Oliveira LS, 2017, MSPHERE, V2, DOI 10.1128/mSphere.00094-17; Dittami SM, 2014, FRONT GENET, V5, DOI 10.3389/fgene.2014.00241; Dittami SM, 2014, MOL ECOL, V23, P1656, DOI 10.1111/mec.12670; Frioux C, 2018, BIOINFORMATICS, V34, P934, DOI 10.1093/bioinformatics/bty588; Goecke F, 2010, MAR ECOL PROG SER, V409, P267, DOI 10.3354/meps08607; Hammer Oyvind, 2001, Palaeontologia Electronica, V4, pUnpaginated; Illumina, 2017, 16S MET SEQ LIB PREP; Karp PD, 2016, BRIEF BIOINFORM, V17, P877, DOI 10.1093/bib/bbv079; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KleinJan H, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.02456; Kozich JJ, 2013, APPL ENVIRON MICROB, V79, P5112, DOI 10.1128/AEM.01043-13; Kreimer A, 2012, BIOINFORMATICS, V28, P2195, DOI 10.1093/bioinformatics/bts323; Le Bail A, 2010, PLANT PHYSIOL, V153, P128, DOI 10.1104/pp.109.149708; Levy R, 2015, BMC BIOINFORMATICS, V16, DOI 10.1186/s12859-015-0588-y; Lindemann SR, 2016, ISME J, V10, P2077, DOI 10.1038/ismej.2016.26; Masella AP, 2012, BMC BIOINFORMATICS, V13, DOI 10.1186/1471-2105-13-31; Metsalu T, 2015, NUCLEIC ACIDS RES, V43, pW566, DOI 10.1093/nar/gkv468; Prigent S, 2017, PLOS COMPUT BIOL, V13, DOI 10.1371/journal.pcbi.1005276; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; Rohwer F, 2002, MAR ECOL PROG SER, V243, P1, DOI 10.3354/meps243001; Schnoes AM, 2009, PLOS COMPUT BIOL, V5, DOI 10.1371/journal.pcbi.1000605; Spoerner M, 2012, J PHYCOL, V48, P1433, DOI 10.1111/j.1529-8817.2012.01231.x; Tapia JE, 2016, FRONT MICROBIOL, V7, DOI [10.3389/fmicb.2016.00197, 10.3389/fmicb.2016.00107]; Tetz G, 2017, GUT PATHOG, V9, DOI 10.1186/s13099-017-0187-8; Thomas F, 2020, ENV MICROBIOL REP, V12, P30, DOI 10.1111/1758-2229.12806; Vallenet D, 2006, NUCLEIC ACIDS RES, V34, P53, DOI 10.1093/nar/gkj406; Wahl M, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00292; Wang Q, 2018, FRONT PLANT SCI, V9, DOI 10.3389/fpls.2018.00313; Wang Q, 2007, APPL ENVIRON MICROB, V73, P5261, DOI 10.1128/AEM.00062-07; Zhou J, 2016, CRIT REV PLANT SCI, V35, P81, DOI 10.1080/07352689.2016.1172461	37	5	5	0	5	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND		2296-7745		FRONT MAR SCI	Front. Mar. Sci.	FEB 21	2020	7								85	10.3389/fmars.2020.00085			11	Environmental Sciences; Marine & Freshwater Biology	Environmental Sciences & Ecology; Marine & Freshwater Biology	KN8PN	WOS:000515107500001		DOAJ Gold, Green Published			2021-04-07	
J	Lyubetsky, VA; Zverkov, OA; Rubanov, LI; Seliverstov, AV				Lyubetsky, Vassily A.; Zverkov, Oleg A.; Rubanov, Lev, I; Seliverstov, Alexandr, V			Optimal Growth Temperature and Intergenic Distances in Bacteria, Archaea, and Plastids of Rhodophytic Branch	BIOMED RESEARCH INTERNATIONAL			English	Article							FUCUS-VESICULOSUS; CYANIDIOSCHYZON-MEROLAE; ECTOCARPUS-SILICULOSUS; PLEUROCLADIA-LACUSTRIS; GENOME SEQUENCE; HEAT-SHOCK; ORIGIN; PHOTOSYNTHESIS; EVOLUTION; SEA	The lengths of intergenic regions between neighboring genes that are convergent, divergent, or unidirectional were calculated for plastids of the rhodophytic branch and complete archaeal and bacterial genomes. Statistically significant linear relationships between any pair of the medians of these three length types have been revealed in each genomic group. Exponential relationships between the optimal growth temperature and each of the three medians have been revealed as well. The leading coefficients of the regression equations relating all pairs of the medians as well as temperature and any of the medians have the same sign and order of magnitude. The results obtained for plastids, archaea, and bacteria are also similar at the qualitative level. For instance, the medians are always low at high temperatures. At low temperatures, the medians tend to statistically significant greater values and scattering. The original model was used to test our hypothesis that the intergenic distances are optimized in particular to decrease the competition of RNA polymerases within the locus that results in transcribing shortened RNAs. Overall, this points to an effect of temperature for both remote and close genomes.	[Lyubetsky, Vassily A.; Zverkov, Oleg A.; Rubanov, Lev, I; Seliverstov, Alexandr, V] Russian Acad Sci, Inst Informat Transmiss Problems, Kharkevich Inst, Bolshoy Karetny 19, Moscow 127051, Russia	Zverkov, OA (corresponding author), Russian Acad Sci, Inst Informat Transmiss Problems, Kharkevich Inst, Bolshoy Karetny 19, Moscow 127051, Russia.	zverkov@iitp.ru	Lyubetsky, Vassily/D-8425-2014; Zverkov, Oleg A./D-8284-2014; Rubanov, Lev/F-8205-2014	Lyubetsky, Vassily/0000-0002-3739-9161; Zverkov, Oleg A./0000-0002-8546-364X; Seliverstov, Alexandr/0000-0003-4746-6396; Rubanov, Lev/0000-0003-3012-2589	RFBRRussian Foundation for Basic Research (RFBR) [18-29-13037]	The reported study was funded by RFBR according to the research project no. 18-29-13037.	Abbondanzieri EA, 2005, BIOPHYS J, V89, pL61, DOI 10.1529/biophysj.105.074195; Aigner S, 2017, EUR J PHYCOL, V52, P238, DOI 10.1080/09670262.2016.1274430; AVILA M, 1986, CAN J BOT, V64, P1867, DOI 10.1139/b86-247; Bergey D. H, 2012, ACTINOBACTERIA, V5; BOLTON JJ, 1982, MAR BIOL, V66, P89, DOI 10.1007/BF00397259; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Boone D.R., 2001, ARCHAEA DEEPLY BRANC, V1, P289; Brandt LD, 2016, ARCHAEA, V2016, DOI 10.1155/2016/2690329; Bricelj VM, 1997, LIMNOL OCEANOGR, V42, P1023, DOI 10.4319/lo.1997.42.5_part_2.1023; Buskey EJ, 1998, J PLANKTON RES, V20, P1553, DOI 10.1093/plankt/20.8.1553; Carvalho AP, 2009, J APPL PHYCOL, V21, P543, DOI 10.1007/s10811-009-9415-z; Chen BB, 2014, MAR BIOL RES, V10, P1019, DOI 10.1080/17451000.2013.872798; Choi HG, 2008, J APPL PHYCOL, V20, P729, DOI 10.1007/s10811-007-9281-5; Chorus I., 1999, TOXIC CYANOBACTERIA; Claquin P, 2008, AQUAT MICROB ECOL, V51, P1, DOI 10.3354/ame01187; Cox CJ, 2008, P NATL ACAD SCI USA, V105, P20356, DOI 10.1073/pnas.0810647105; DAVISON IR, 1991, J PHYCOL, V27, P2, DOI 10.1111/j.0022-3646.1991.00002.x; Dennis PP, 2009, J BACTERIOL, V191, P3740, DOI 10.1128/JB.00128-09; Donaher N, 2009, GENOME BIOL EVOL, V1, P439, DOI 10.1093/gbe/evp047; Endo H., 2013, American Journal of Plant Sciences, V4, P14; Fawley KP, 2007, PROTIST, V158, P325, DOI 10.1016/j.protis.2007.03.003; Fielding SR, 2013, LIMNOL OCEANOGR, V58, P663, DOI 10.4319/lo.2013.58.2.0663; Figueroa RI, 2009, PROTIST, V160, P285, DOI 10.1016/j.protis.2008.12.003; Freire I, 2016, AQUACULTURE, V459, P124, DOI 10.1016/j.aquaculture.2016.03.015; Gao X, 2013, J APPL PHYCOL, V25, P567, DOI 10.1007/s10811-012-9891-4; Gershgorin RA, 2017, LIFE-BASEL, V7, DOI 10.3390/life7010009; Gigova L, 2012, J PHYCOL, V48, P85, DOI 10.1111/j.1529-8817.2011.01088.x; Gowers DM, 2005, P NATL ACAD SCI USA, V102, P15883, DOI 10.1073/pnas.0505378102; Graiff A, 2015, J EXP MAR BIOL ECOL, V471, P8, DOI 10.1016/j.jembe.2015.05.009; Halford SE, 2009, BIOCHEM SOC T, V37, P343, DOI 10.1042/BST0370343; Ichinomiya M, 2016, ISME J, V10, P2419, DOI 10.1038/ismej.2016.38; Ichinomiya M, 2015, AQUAT MICROB ECOL, V75, P207, DOI 10.3354/ame01756; Imanian B, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0010711; Imbs TI, 2009, CHEM NAT COMPD+, V45, P786, DOI 10.1007/s10600-010-9507-7; Jain K, 2015, GENOME BIOL EVOL, V7, P367, DOI 10.1093/gbe/evu290; Janouskovec J, 2015, P NATL ACAD SCI USA, V112, P10200, DOI 10.1073/pnas.1423790112; Janouskovec J, 2010, P NATL ACAD SCI USA, V107, P10949, DOI 10.1073/pnas.1003335107; Jiang Hong-bo, 2009, Ying Yong Sheng Tai Xue Bao, V20, P185; Kadnikov VV, 2017, MICROBIOLOGY+, V86, P412, DOI 10.1134/S0026261717030079; KLEINSCHMIDT MG, 1970, PLANT PHYSIOL, V46, P290, DOI 10.1104/pp.46.2.290; KLEINSCHMIDT MG, 1970, PLANT PHYSIOL, V46, P286, DOI 10.1104/pp.46.2.286; Kobayashi Y, 2014, GENOME BIOL EVOL, V6, P2731, DOI 10.1093/gbe/evu216; Koonin EV, 2015, PHILOS T R SOC B, V370, DOI 10.1098/rstb.2014.0333; Korolev SA, 2016, BIOL DIRECT, V11, DOI 10.1186/s13062-016-0123-8; Krieg N. R., 2009, FIRMICUTES, V3; Krieg N. R., 2011, BACTEROIDETES SPIROC, V4; Lazar CS, 2017, ARCHAEA, V2017, DOI 10.1155/2017/2136287; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; Li GQ, 2016, SCI REP-UK, V6, DOI 10.1038/srep30009; Li XS, 2011, AQUAC RES, V42, P1079, DOI 10.1111/j.1365-2109.2010.02691.x; Lira B., 2017, BR MICROBIOL RES J, V18, P1, DOI DOI 10.9734/MRJI/2017/30342; Liu F, 2016, J APPL PHYCOL, V28, P1145, DOI 10.1007/s10811-015-0675-5; Loiko NG, 2017, MICROBIOLOGY+, V86, P560, DOI 10.1134/S0026261717050149; Long JD, 2007, J PLANKTON RES, V29, P769, DOI 10.1093/plankt/fbm058; Lyubetsky V, 2016, BMC BIOINFORMATICS, V17, DOI 10.1186/s12859-016-0878-z; Lyubetsky VA, 2012, BIOL DIRECT, V7, DOI 10.1186/1745-6150-7-26; Lyubetsky VA, 2011, BIOL DIRECT, V6, DOI 10.1186/1745-6150-6-3; Ma YT, 2016, ARCHAEA, V2016, DOI 10.1155/2016/9278929; Martin WF, 2015, PHILOS T R SOC B, V370, DOI 10.1098/rstb.2014.0330; Matsuzaki M, 2004, NATURE, V428, P653, DOI 10.1038/nature02398; Mirny L, 2009, J PHYS A-MATH THEOR, V42, DOI 10.1088/1751-8113/42/43/434013; Mujer CV, 1996, P NATL ACAD SCI USA, V93, P12333, DOI 10.1073/pnas.93.22.12333; Nakabayashi N, 2002, NIPPON SUISAN GAKK, V68, P659; Nam-Gil K, 1999, HYDROBIOLOGIA, V399, P127; Notoya M, 1999, HYDROBIOLOGIA, V399, P121; NOTOYA M., 1998, ALGAE, V13, P207; Nygard CA, 2008, EUR J PHYCOL, V43, P253, DOI 10.1080/09670260802172627; Ono K, 2000, AQUAC RES, V31, P427, DOI 10.1046/j.1365-2109.2000.00463.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Place AR, 2012, HARMFUL ALGAE, V14, P179, DOI 10.1016/j.hal.2011.10.021; REYNOLDS CS, 1987, ADV BOT RES, V13, P67; Rogozin IB, 2002, NUCLEIC ACIDS RES, V30, P4264, DOI 10.1093/nar/gkf549; RYALS J, 1982, J BACTERIOL, V151, P879, DOI 10.1128/JB.151.2.879-887.1982; Sadovskaya TA, 2009, MOL BIOL+, V43, P552, DOI 10.1134/S0026893309040037; Satoh A, 2013, BIORESOURCE TECHNOL, V137, P132, DOI [10.1016/j.biortech.2013.03.087, 10.1016/j.biortech.2]; Schelkunov M. I., 2010, P 33 C INF TECHN SYS, P382; Seliverstov AV, 2015, BIOMED RES INT, V2015, DOI 10.1155/2015/452958; Smith WO, 1999, J PLANKTON RES, V21, P1519, DOI 10.1093/plankt/21.8.1519; STROMGREN T, 1977, J EXP MAR BIOL ECOL, V29, P181, DOI 10.1016/0022-0981(77)90047-8; Wehr JD, 2013, WEST N AM NATURALIST, V73, P148, DOI 10.3398/064.073.0204; Wei L, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-534; WILCE RT, 1966, J PHYCOL, V2, P57, DOI 10.1111/j.1529-8817.1966.tb04595.x; Yakoubou S., 2005, THE PROTEOBACTERIA, V2; Yoon HS, 2002, P NATL ACAD SCI USA, V99, P15507, DOI 10.1073/pnas.242379899; Yuan C. Y., 2014, ADV MAT RES, V989-994, P747, DOI [10.4028/www.scientific.net/amr.989-994.747, DOI 10.4028/WWW.SCIENTIFIC.NET/AMR.989-994.747]; Zakrzewska K, 2012, CURR OPIN STRUC BIOL, V22, P160, DOI 10.1016/j.sbi.2012.01.004; Zaremba-Niedzwiedzka K, 2017, NATURE, V541, P353, DOI 10.1038/nature21031	87	1	1	1	1	HINDAWI LTD	LONDON	ADAM HOUSE, 3RD FLR, 1 FITZROY SQ, LONDON, W1T 5HF, ENGLAND	2314-6133	2314-6141		BIOMED RES INT	Biomed Res. Int.	JAN 18	2020	2020								3465380	10.1155/2020/3465380			10	Biotechnology & Applied Microbiology; Medicine, Research & Experimental	Biotechnology & Applied Microbiology; Research & Experimental Medicine	KG3ZA	WOS:000509880900003	32025518	DOAJ Gold, Green Published			2021-04-07	
J	Karimi, E; Geslain, E; KleinJan, H; Tanguy, G; Legeay, E; Corre, E; Dittami, SM				Karimi, Elham; Geslain, Enora; KleinJan, Hetty; Tanguy, Gwenn; Legeay, Erwan; Corre, Erwan; Dittami, Simon M.			Genome Sequences of 72 Bacterial Strains Isolated from Ectocarpus subulatus: A Resource for Algal Microbiology	GENOME BIOLOGY AND EVOLUTION			English	Article						brown algae; holobiont; alga-associated bacteria; biosynthetic gene clusters; detoxification; metabolic networks	BROWN; MICROORGANISMS; SILICULOSUS; EVOLUTION; CYANATE	Brown algae are important primary producers and ecosystem engineers in the ocean, and Ectocarpus has been established as a laboratory model for this lineage. Like most multicellular organisms, Ectocarpus is associated with a community of microorganisms, a partnership frequently referred to as holobiont due to the tight interconnections between the components. Although genomic resources for the algal host are well established, its associated microbiome is poorly characterized from a genomic point of view, limiting the possibilities of using these types of data to study host-microbe interactions. To address this gap in knowledge, we present the annotated draft genome sequences of seventy-two cultivable Ectocarpus-associated bacteria. A screening of gene clusters related to the production of secondary metabolites revealed terpene, bacteriocin, NRPS, PKS-t3, siderophore, PKS-t1, and homoserine lactone clusters to be abundant among the sequenced genomes. These compounds may be used by the bacteria to communicate with the host and other microbes. Moreover, detoxification and provision of vitamin B pathways have been observed in most sequenced genomes, highlighting potential contributions of the bacterial metabolism toward host fitness and survival. The genomes sequenced in this study form a valuable resource for comparative genomic analyses and evolutionary surveys of alga-associated bacteria. They help establish Ectocarpus as a model for brown algal holobionts and will enable the research community to produce testable hypotheses about the molecular interactions within this complex system.	[Karimi, Elham; Geslain, Enora; KleinJan, Hetty; Dittami, Simon M.] Sorbonne Univ, CNRS, UMR 8227, Stn Biol Roscoff,Integrat Biol Marine Models, Roscoff, France; [Geslain, Enora; Tanguy, Gwenn; Legeay, Erwan; Corre, Erwan] Sorbonne Univ, CNRS, FR2424, Stn Biol Roscoff, Roscoff, France; [KleinJan, Hetty] CEBEDEAU, Res & Expertise Ctr Water, Liege, Belgium	Karimi, E (corresponding author), Sorbonne Univ, CNRS, UMR 8227, Stn Biol Roscoff,Integrat Biol Marine Models, Roscoff, France.	elham.karimi@sb-roscoff.fr	corre, erwan/O-4669-2019	corre, erwan/0000-0001-6354-2278	Investissement d'Avenir programFrench National Research Agency (ANR) [ANR-10-INBS09, ANR-11-INBS-0013]; CNRS Momentum call; ANR project IDEALGFrench National Research Agency (ANR) [ANR-10-BTBR-04]; European Union's Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie grant [624575]	We appreciate the LABGeM (CEA/Genoscope & CNRS UMR8030), the France Genomique and French Bioinformatics Institute national infrastructures (funded as part of Investissement d'Avenir program managed by Agence Nationale pour la Recherche, contracts ANR-10-INBS09 and ANR-11-INBS-0013) for their technical support within the MicroScope annotation platform and thank to Sylvie Rousvoal for help with DNA extractions.; This study was supported partially by the CNRS Momentum call, the ANR project IDEALG [ANR-10-BTBR-04] "Investissements d'Avenir, Biotechnologies-Bioressources," and the European Union's Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie grant agreement [624575 (ALFF)]. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.	Andrews S., 2010, BABRAHAM BIOINFORMAT; Bankevich A, 2012, J COMPUT BIOL, V19, P455, DOI 10.1089/cmb.2012.0021; Behringer G, 2018, FRONT MICROBIOL, V9, DOI 10.3389/fmicb.2018.00659; Blin K, 2017, NUCLEIC ACIDS RES, V45, pW36, DOI 10.1093/nar/gkx319; Brodie J, 2017, TRENDS PLANT SCI, V22, P726, DOI 10.1016/j.tplants.2017.05.005; Burgunter-Delamare B, 2019, PREPRINT, DOI [10.1101/813683, DOI 10.1101/813683]; Caspi R, 2018, NUCLEIC ACIDS RES, V46, pD633, DOI 10.1093/nar/gkx935; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cosse A., 2007, ADV BOT RES, V46, P221, DOI DOI 10.1016/S0065-2296(07)46006-2; Croft MT, 2005, NATURE, V438, P90, DOI 10.1038/nature04056; Dittami SM, 2016, ISME J, V10, P51, DOI 10.1038/ismej.2015.104; Dittami SM, 2014, MOL ECOL, V23, P1656, DOI 10.1111/mec.12670; Dittami SM, 2019, BIORXIV821579, DOI [10.1101/821579, DOI 10.1101/821579]; Douglas AE, 2016, MBIO, V7, DOI [10.1128/mBio.02099, 10.1128/mBio.02099-15]; Frioux C, 2018, BIOINFORMATICS, V34, P934, DOI 10.1093/bioinformatics/bty588; Gershenzon J, 2007, NAT CHEM BIOL, V3, P408, DOI 10.1038/nchembio.2007.5; Kamennaya NA, 2008, LIMNOL OCEANOGR, V53, P2485, DOI 10.4319/lo.2008.53.6.2485; Kanehisa M, 2008, NUCLEIC ACIDS RES, V36, pD480, DOI 10.1093/nar/gkm882; KIM SK, 2015, HDB MARINE MICROALGA, P1; KleinJan H, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.02456; Klemetsen T, 2018, NUCLEIC ACIDS RES, V46, pD692, DOI 10.1093/nar/gkx1036; Koru E, 2013, SEAWEEDS FOOD IND AP, P735; Li RQ, 2010, GENOME RES, V20, P265, DOI 10.1101/gr.097261.109; Li YH, 2012, SENSORS-BASEL, V12, P2519, DOI 10.3390/s120302519; Nasrolahi A, 2012, FEMS MICROBIOL ECOL, V81, P583, DOI 10.1111/j.1574-6941.2012.01384.x; Netzker T, 2015, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.00299; Paix B, 2019, ENVIRON MICROBIOL, V21, P3346, DOI 10.1111/1462-2920.14617; Palatinszky M, 2015, NATURE, V524, P105, DOI 10.1038/nature14856; Saez LP, 2019, INT J MOL SCI, V20, DOI 10.3390/ijms20123008; Parks DH, 2015, GENOME RES, V25, P1043, DOI 10.1101/gr.186072.114; PEDERSEN M, 1968, NATURE, V218, P776, DOI 10.1038/218776a0; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Raja A., 2013, INT J CURRENT MICROB, V2, P222; REASONER DJ, 1985, APPL ENVIRON MICROB, V49, P1; Richter M, 2016, BIOINFORMATICS, V32, P929, DOI 10.1093/bioinformatics/btv681; Robertsen E., 2017, F1000RESEARCH, V6, DOI [10.12688/f1000research.10443.1, DOI 10.12688/F1000RESEARCH.10443.1]; Susilowati R, 2015, PROCEDIA ENVIRON SCI, V23, P240, DOI 10.1016/j.proenv.2015.01.036; Tang YZ, 2010, P NATL ACAD SCI USA, V107, P20756, DOI 10.1073/pnas.1009566107; Tapia JE, 2016, FRONT MICROBIOL, V7, DOI [10.3389/fmicb.2016.00197, 10.3389/fmicb.2016.00107]; Vallenet D, 2017, NUCLEIC ACIDS RES, V45, pD517, DOI 10.1093/nar/gkw1101; WEISBURG WG, 1991, J BACTERIOL, V173, P697, DOI 10.1128/JB.173.2.697-703.1991; West John A., 1996, Muelleria, V9, P29; Wiese J, 2009, MAR BIOTECHNOL, V11, P287, DOI 10.1007/s10126-008-9143-4; Yamada Y, 2015, P NATL ACAD SCI USA, V112, P857, DOI 10.1073/pnas.1422108112; Zilber-Rosenberg I, 2008, FEMS MICROBIOL REV, V32, P723, DOI 10.1111/j.1574-6976.2008.00123.x	46	3	3	0	2	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	1759-6653			GENOME BIOL EVOL	Genome Biol. Evol.	JAN	2020	12	1					3647	3655		10.1093/gbe/evz278			9	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	KY8YA	WOS:000522860800009	31841132	DOAJ Gold, Green Published			2021-04-07	
J	Shao, ZR; Zhang, PY; Lu, C; Li, SX; Chen, ZH; Wang, XL; Duan, DL				Shao, Zhanru; Zhang, Pengyan; Lu, Chang; Li, Shaoxuan; Chen, Zhihang; Wang, Xiuliang; Duan, Delin			Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis	BMC GENOMICS			English	Article						Alginate; Mannitol; Transcriptome; Regulatory networks; Growth; Development; Saccharina japonica	BROWN ALGA ECTOCARPUS; EXTRACELLULAR-MATRIX; EXPRESSION ANALYSIS; GENE; INSIGHTS; METABOLISM; EVOLUTION; PROVIDE; POLYSACCHARIDES; DEHYDROGENASE	Background Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in the brown seaweed Saccharina japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown. Results With WGCNA and trend analysis of 20,940 known genes and 4264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and "imm upregulated 3" gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and Fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6-phosphate, contributing to high content of mannitol in the basal blade. Conclusions The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. japonica developments.	[Shao, Zhanru; Zhang, Pengyan; Lu, Chang; Chen, Zhihang; Wang, Xiuliang; Duan, Delin] Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China; [Shao, Zhanru; Zhang, Pengyan; Lu, Chang; Chen, Zhihang; Wang, Xiuliang; Duan, Delin] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; [Zhang, Pengyan] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China; [Lu, Chang; Chen, Zhihang] Univ Chinese Acad Sci, Beijing 100093, Peoples R China; [Li, Shaoxuan] Qingdao Acad Agr Sci, Qingdao 266100, Shandong, Peoples R China; [Duan, Delin] Qingdao Brightmoon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Shandong, Peoples R China	Duan, DL (corresponding author), Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China.; Duan, DL (corresponding author), Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China.	dlduan@qdio.ac.cn	Li, Shaoxuan/AAB-3642-2020; Duan, Delin/G-9002-2011	duan, delin/0000-0001-5356-5839; Wang, Xiuliang/0000-0003-4262-0083	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41806175]; Shandong Key Research and Development Program [2018GHY115023]; Qingdao National Laboratory for Marine Science and Technology Project [2018ASKJ03]; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances [SKL-BASS1702]	This work was supported by the National Natural Science Foundation of China (no. 41806175), Shandong Key Research and Development Program (2018GHY115023), Qingdao National Laboratory for Marine Science and Technology Project (No.2018ASKJ03), and Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances (SKL-BASS1702).	Attwooll C, 2004, EMBO J, V23, P4709, DOI 10.1038/sj.emboj.7600481; Ballif J, 2011, PLANT PHYSIOL BIOCH, V49, P579, DOI 10.1016/j.plaphy.2011.01.013; Bonente G, 2012, J BIOL CHEM, V287, P5833, DOI 10.1074/jbc.M111.304279; Bonin P, 2015, PHYTOCHEMISTRY, V117, P509, DOI 10.1016/j.phytochem.2015.07.015; Chan CX, 2012, J PHYCOL, V48, P1328, DOI 10.1111/j.1529-8817.2012.01229.x; Chi S, 2018, CURR GENET, V64, P259, DOI 10.1007/s00294-017-0733-4; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Donati I, 2009, MICROBIOL MONOGR, V13, P1, DOI 10.1007/978-3-540-92679-5_1; Ernst J, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-191; Fischl R, 2016, GLYCOBIOLOGY, V26, P973, DOI 10.1093/glycob/cww040; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Groisillier A, 2014, J EXP BOT, V65, P559, DOI 10.1093/jxb/ert405; Haga N, 2007, DEVELOPMENT, V134, P1101, DOI 10.1242/dev.02801; Huang XY, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0170855; Inoue A, 2016, ALGAL RES, V16, P282, DOI 10.1016/j.algal.2016.03.030; Ito T, 2000, PLANT J, V22, P257, DOI 10.1046/j.1365-313x.2000.00728.x; Iwamoto K, 2005, MAR BIOTECHNOL, V7, P407, DOI 10.1007/s10126-005-0029-4; JI MH, 1984, HYDROBIOLOGIA, V116, P554; Kalve S, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00362; Kanehisa M, 2008, NUCLEIC ACIDS RES, V36, pD480, DOI 10.1093/nar/gkm882; Kato K, 2009, PLANT PHYSIOL, V149, P1945, DOI 10.1104/pp.109.135582; Kettenberger H, 2003, CELL, V114, P347, DOI 10.1016/S0092-8674(03)00598-1; Kim D, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-4-r36; Klettner A, 2016, MAR DRUGS, V14, DOI 10.3390/md14020031; Kupper FC, 1998, PLANTA, V207, P163, DOI 10.1007/s004250050469; Landa-Cansigno C, 2017, J APPL PHYCOL, V29, P2605, DOI 10.1007/s10811-017-1195-2; Langfelder P, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-559; LAYCOCK RA, 1974, MAR BIOL, V25, P223, DOI 10.1007/BF00394968; Li B, 2011, BMC BIOINFORMATICS, V12, DOI 10.1186/1471-2105-12-323; Liu JX, 2010, PLANT CELL, V22, P782, DOI 10.1105/tpc.109.072173; Lu MQ, 2014, J BIOBASED MATER BIO, V8, P415, DOI 10.1166/jbmb.2014.1455; Manns D, 2017, J APPL PHYCOL, V29, P1493, DOI 10.1007/s10811-017-1056-z; MCKEE JWA, 1992, J APPL PHYCOL, V4, P357, DOI 10.1007/BF02185794; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Moradali MF, 2018, SPRING SER BIOMAT S, V11, P1, DOI 10.1007/978-981-10-6910-9_1; Moradali MF, 2017, APPL ENVIRON MICROB, V83, DOI 10.1128/AEM.03499-16; PERCIVAL EGV, 1948, J SOC CHEM IND-L, V67, P420, DOI 10.1002/jctb.5000671106; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Robinson MD, 2010, BIOINFORMATICS, V26, P139, DOI 10.1093/bioinformatics/btp616; Rousvoal S, 2011, PLANTA, V233, P261, DOI 10.1007/s00425-010-1295-6; Shannon P, 2003, GENOME RES, V13, P2498, DOI 10.1101/gr.1239303; Shao ZR, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0097935; Starko S, 2018, EUR J PHYCOL, V53, P307, DOI 10.1080/09670262.2018.1449013; Stephenson TJ, 2010, FUNCT INTEGR GENOMIC, V10, P265, DOI 10.1007/s10142-010-0158-3; Stirnberg P, 2012, BMC PLANT BIOL, V12, DOI 10.1186/1471-2229-12-160; Tenhaken R, 2011, J BIOL CHEM, V286, P16707, DOI 10.1074/jbc.M111.230979; Tonon T, 2017, NEW PHYTOL, V213, P1573, DOI 10.1111/nph.14358; Trapnell C, 2012, NAT PROTOC, V7, P562, DOI 10.1038/nprot.2012.016; TSENG C K, 1986, FAO (Food and Agriculture Organization of the United Nations) Fisheries Technical Paper, P239; [吴海一 Wu Haiyi], 2015, [海洋科学, Marine Sciences], V39, P35; [姚海芹 Yao Haiqin], 2016, [食品科学, Food Science], V37, P95; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zeng FM, 2008, ACAD PERIODICAL PROD, V6, P60; Zhang PY, 2018, J APPL PHYCOL, V30, P2687, DOI 10.1007/s10811-018-1460-z; Zhang PY, 2016, BMC PLANT BIOL, V16, DOI 10.1186/s12870-016-0750-3; Zia KM, 2015, INT J BIOL MACROMOL, V79, P377, DOI 10.1016/j.ijbiomac.2015.04.076; Zubia M, 2008, J APPL PHYCOL, V20, P1033, DOI 10.1007/s10811-007-9303-3	59	8	9	3	12	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2164			BMC GENOMICS	BMC Genomics	DEC 12	2019	20	1							975	10.1186/s12864-019-6366-x			15	Biotechnology & Applied Microbiology; Genetics & Heredity	Biotechnology & Applied Microbiology; Genetics & Heredity	JW0YU	WOS:000502786600002	31830918	DOAJ Gold, Green Published			2021-04-07	
J	Monteiro, C; Heinrich, S; Bartsch, I; Valentin, K; Corre, E; Collen, J; Harms, L; Glockner, G; Bischof, K				Monteiro, Catia; Heinrich, Sandra; Bartsch, Inka; Valentin, Klaus; Corre, Erwan; Collen, Jonas; Harms, Lars; Gloeckner, Gernot; Bischof, Kai			Temperature Modulates Sex-Biased Gene Expression in the Gametophytes of the Kelp Saccharina latissima	FRONTIERS IN MARINE SCIENCE			English	Article						brown algae; gametophyte; gene expression; kelp; life cycle; sex; temperature; transcriptomics	BROWN ALGA ECTOCARPUS; LAMINARIA-SACCHARINA; LIFE-HISTORY; UNDARIA-PINNATIFIDA; CHELATED IRON; GROWTH; EVOLUTION; LIGHT; PHAEOPHYCEAE; REPRODUCTION	Saccharina latissima is an economically and ecologically relevant kelp species in Europe and North America. In kelps, the sexuality is expressed during the haploid life stage and the microscopic gametophytes exhibit significant sexual dimorphism. To understand the sex-dependent impact of temperature on the gametophyte stage, we analyzed for the first time, gene expression profiles of male and female gametophytes at three different temperatures (4, 12, and 20 degrees C) characteristic for the species distribution range by using RNA-sequencing. We identified several differentially expressed genes (DEGs) between sexes; while female biased genes were enriched in general metabolism and energy production, male biased genes function within cell cycle and signaling. In our study, temperature modulated sex-biased gene expression, with only a small percentage of DEGs consistently male (7%) or female-biased (12%) at the three temperatures. Female gametophytes responded stronger to higher temperatures than males, suggesting that males are more heat tolerant. Differences between S. latissima and other brown algal gender-dependent gene expression might mirror the different evolutionary and ecological contexts. Genomic information on kelp gametophyte is still scarce and thus this study adds to our knowledge on sex differences in abiotic stress responses in macroalgae at the transcriptomic level.	[Monteiro, Catia; Bischof, Kai] Univ Bremen, Fac Biol Chem, Marine Bot, Bremen, Germany; [Monteiro, Catia; Corre, Erwan] Sorbonne Univ UPMC, CNRS FR2424, Stn Biol Roscoff, Plateforme ABiMS, Roscoff, France; [Monteiro, Catia; Collen, Jonas] Sorbonne Univ UPMC, Stn Biol Roscoff, CNRS, Integrat Biol Marine Models LBI2M, Roscoff, France; [Heinrich, Sandra] Univ Hamburg, Inst Plant Sci & Microbiol, Hamburg, Germany; [Bartsch, Inka; Valentin, Klaus; Harms, Lars] Helmholtz Ctr Polar & Marine Res, Alfred Wegener Inst, Bremerhaven, Germany; [Gloeckner, Gernot] Univ Cologne, Med Fac, Inst Biochem 1, Cologne, Germany	Monteiro, C (corresponding author), Univ Bremen, Fac Biol Chem, Marine Bot, Bremen, Germany.; Monteiro, C (corresponding author), Sorbonne Univ UPMC, CNRS FR2424, Stn Biol Roscoff, Plateforme ABiMS, Roscoff, France.; Monteiro, C (corresponding author), Sorbonne Univ UPMC, Stn Biol Roscoff, CNRS, Integrat Biol Marine Models LBI2M, Roscoff, France.	monteiro@uni-bremen.de	corre, erwan/O-4669-2019; Monteiro, Catia/AAM-9813-2020; Glockner, Gernot/A-7800-2010	corre, erwan/0000-0001-6354-2278; Monteiro, Catia/0000-0002-5392-072X; Glockner, Gernot/0000-0002-9061-1061	German Research Foundation within the ERA-Net Cofund BiodivERsA 3 program MARFOR [ANR-16-EBI3-0005-01]; MARES Joint Doctoral Programme on Marine Ecosystem Health & Conservation through Erasmus Mundus [MARES_14_09]; Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research (Bremerhaven, Germany)	This work was supported by the German Research Foundation for funding within the ERA-Net Cofund BiodivERsA 3 program MARFOR (ANR-16-EBI3-0005-01). Further funding was provided by the MARES Joint Doctoral Programme on Marine Ecosystem Health & Conservation funded through Erasmus Mundus (grant number MARES_14_09) and the Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research (Bremerhaven, Germany).	Andrews S, 2014, MAR ECOL PROG SER, V495, P119, DOI 10.3354/meps10567; Araujo RM, 2016, BIODIVERS CONSERV, V25, P1319, DOI 10.1007/s10531-016-1141-7; Assis J, 2017, SCI REP-UK, V7, DOI 10.1038/srep44348; Athar A, 2019, NUCLEIC ACIDS RES, V47, pD711, DOI 10.1093/nar/gky964; Barradas A, 2011, CAH BIOL MAR, V52, P435; Barrett SCH, 2013, J EXP BOT, V64, P67, DOI 10.1093/jxb/ers308; Bartsch I, 2018, DERIVATION CLONAL ST, P61, DOI DOI 10.1201/B21460-3; Bell G, 1997, BIOL J LINN SOC, V60, P21, DOI 10.1111/j.1095-8312.1997.tb01481.x; Bi Yan-Hui, 2014, Algological Studies, V145, P65, DOI 10.1127/1864-1318/2014/0145; Blekhman R, 2010, GENOME RES, V20, P180, DOI 10.1101/gr.099226.109; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; BOLTON JJ, 1982, MAR BIOL, V66, P89, DOI 10.1007/BF00397259; Borcard D, 2011, USE R, P1, DOI 10.1007/978-1-4419-7976-6; Breitbach G, 2016, OCEAN SCI, V12, P909, DOI 10.5194/os-12-909-2016; Broch OJ, 2019, FRONT MAR SCI, V5, DOI 10.3389/fmars.2018.00529; Bryant DM, 2017, CELL REP, V18, P762, DOI 10.1016/j.celrep.2016.12.063; Buchfink B, 2015, NAT METHODS, V12, P59, DOI 10.1038/nmeth.3176; Charrier B, 2017, NEW PHYTOL, V216, P967, DOI 10.1111/nph.14728; Coelho SM, 2019, NEW PHYTOL, V222, P1751, DOI 10.1111/nph.15694; Coelho Susana M., 2000, Journal of Aquatic Ecosystem Stress and Recovery, V7, P317, DOI 10.1023/A:1009916129009; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; de Bettignies T, 2018, FRONT MAR SCI, V5, DOI 10.3389/fmars.2018.00218; Destombe C, 2011, CAH BIOL MAR, V52, P385; DIECK IT, 1993, MAR ECOL PROG SER, V100, P253; Dixon P, 2003, J VEG SCI, V14, P927, DOI 10.1111/j.1654-1103.2003.tb02228.x; Dubin MJ, 2018, CURR OPIN PLANT BIOL, V42, P23, DOI 10.1016/j.pbi.2018.01.003; Edwards MS, 2000, ECOLOGY, V81, P2404, DOI 10.2307/177463; EGAN B, 1989, J EXP MAR BIOL ECOL, V129, P1, DOI 10.1016/0022-0981(89)90059-2; Ellegren H, 2007, NAT REV GENET, V8, P689, DOI 10.1038/nrg2167; Fischl R, 2016, GLYCOBIOLOGY, V26, P973, DOI 10.1093/glycob/cww040; Forbord S, 2012, J APPL PHYCOL, V24, P393, DOI 10.1007/s10811-011-9784-y; Fredersdorf J, 2009, OECOLOGIA, V160, P483, DOI 10.1007/s00442-009-1326-9; Gao X, 2019, MAR POLLUT BULL, V142, P315, DOI 10.1016/j.marpolbul.2019.03.063; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Hays GC, 2017, P ROY SOC B-BIOL SCI, V284, DOI 10.1098/rspb.2016.2576; Heinrich S, 2012, EUR J PHYCOL, V47, P83, DOI 10.1080/09670262.2012.660639; Hou YL, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.01073; HSIAO SIC, 1973, J PHYCOL, V9, P160, DOI 10.1111/j.1529-8817.1973.tb04073.x; Hu Z-L, 2008, ONLINE J BIOINFORMAT, V9, P108, DOI DOI 10.1186/1471-2105-13-134; Hurd C. L., 2014, LIFE HIST REPROD MOR; Hurd C. L., 2014, SEAWEED THALI CELLS; Izquierdo JL, 2002, HELGOLAND MAR RES, V55, P285, DOI 10.1007/s10152-001-0087-6; Jackson C, 2017, J PHYCOL, V53, P1, DOI 10.1111/jpy.12465; JANZEN FJ, 1994, P NATL ACAD SCI USA, V91, P7487, DOI 10.1073/pnas.91.16.7487; Kim JK, 2015, MAR ECOL PROG SER, V531, P155, DOI 10.3354/meps11331; de Araujo AVL, 2019, BIOMED RES INT, V2019, DOI 10.1155/2019/7106951; Lane CE, 2006, J PHYCOL, V42, P493, DOI 10.1111/j.1529-8817.2006.00204.x; Leal PP, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-32899-w; LEE JA, 1986, J PHYCOL, V22, P276, DOI 10.1111/j.1529-8817.1986.tb00024.x; LEE JA, 1988, J PHYCOL, V24, P181, DOI 10.1111/j.1529-8817.1988.tb04232.x; Lewis RJ, 2013, PHYCOL RES, V61, P46, DOI 10.1111/j.1440-1835.2012.00667.x; Li HR, 2020, PHYSIOL PLANTARUM, V168, P5, DOI 10.1111/ppl.13009; Li J, 2013, CHIN J OCEANOL LIMN, V31, P774, DOI 10.1007/s00343-013-2207-y; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Lipinska AP, 2017, GENOME BIOL, V18, DOI 10.1186/s13059-017-1201-7; Lipinska AP, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-909; Livanos P, 2012, PLANT SIGNAL BEHAV, V7, P771, DOI 10.4161/psb.20530; Livanos P, 2012, CYTOSKELETON, V69, P1, DOI 10.1002/cm.20538; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; LUNING K, 1978, MAR BIOL, V45, P297, DOI 10.1007/BF00391816; LUNING K, 1980, J PHYCOL, V16, P1; LUNING K, 1975, MAR BIOL, V29, P195, DOI 10.1007/BF00391846; LUNING K, 1978, Z PFLANZENPHYSIOL, V89, P333; Luthringer R, 2014, PERSPECT PHYCOL, V1, P11, DOI DOI 10.1127/2198-011X/2014/0002; Makarevitch I, 2015, PLOS GENET, V11, DOI [10.1371/journal.pgen.1004915, 10.1371/journal.pgen.1005566]; Martins MJF, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-294; Martins N, 2019, EUR J PHYCOL, V54, P548, DOI 10.1080/09670262.2019.1613571; Martins N, 2017, BOT MAR, V60, P109, DOI 10.1515/bot-2016-0094; Maynard D, 2018, J EXP BOT, V69, P5341, DOI 10.1093/jxb/ery316; Metta M, 2006, GENETICS, V174, P411, DOI 10.1534/genetics.106.057414; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; MOTOMURA T, 1981, B JPN SOC SCI FISH, V47, P1535; Nelson WA, 2005, J APPL PHYCOL, V17, P23, DOI 10.1007/s10811-005-5521-8; Oppliger LV, 2011, J PHYCOL, V47, P5, DOI 10.1111/j.1529-8817.2010.00930.x; Ospina-Alvarez N, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002837; Park J, 2017, BOT MAR, V60, P39, DOI 10.1515/bot-2016-0103; Patro R, 2017, NAT METHODS, V14, P417, DOI 10.1038/nmeth.4197; Pearson GA, 2019, PLOS ONE, V14, DOI 10.1371/journal.pone.0219723; Pehlke C, 2008, CLIM RES, V37, P135, DOI 10.3354/cr00767; Rhodes D, 2015, NUCLEIC ACIDS RES, V43, P8627, DOI 10.1093/nar/gkv862; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Ritter A, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0173315; Roleda MY, 2016, POLAR BIOL, V39, P1967, DOI 10.1007/s00300-015-1813-x; Sabouri N, 2017, CURR GENET, V63, P621, DOI 10.1007/s00294-016-0675-2; Schiel DR, 2006, ANNU REV ECOL EVOL S, V37, P343, DOI 10.1146/annurev.ecolsys.37.091305.110251; Shan TF, 2015, J APPL PHYCOL, V27, P1011, DOI 10.1007/s10811-014-0393-4; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; Teagle H, 2017, J EXP MAR BIOL ECOL, V492, P81, DOI 10.1016/j.jembe.2017.01.017; Martin MV, 2013, PLANT SIGNAL BEHAV, V8, DOI 10.4161/psb.25714; Waterhouse RM, 2018, MOL BIOL EVOL, V35, P543, DOI 10.1093/molbev/msx319; Wuest SE, 2010, CURR BIOL, V20, P506, DOI 10.1016/j.cub.2010.01.051; Yang X, 2006, GENOME RES, V16, P995, DOI 10.1101/gr.5217506; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Young MD, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-2-r14; Zinn KE, 2010, J EXP BOT, V61, P1959, DOI 10.1093/jxb/erq053	95	3	3	3	5	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND		2296-7745		FRONT MAR SCI	Front. Mar. Sci.	DEC 12	2019	6								769	10.3389/fmars.2019.00769			12	Environmental Sciences; Marine & Freshwater Biology	Environmental Sciences & Ecology; Marine & Freshwater Biology	JW3RT	WOS:000502973100001		DOAJ Gold			2021-04-07	
J	Tang, L; Qiu, LP; Liu, C; Du, GY; Mo, ZL; Tang, XH; Mao, YX				Tang, Lei; Qiu, Liping; Liu, Cong; Du, Guoying; Mo, Zhaolan; Tang, Xianghai; Mao, Yunxiang			Transcriptomic Insights into Innate Immunity Responding to Red Rot Disease in Red Alga Pyropia yezoensis	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES			English	Article						Transcriptome; Pyropia yezoensis; innate immune systems; infection; Pythium porphyrae	PROGRAMMED CELL-DEATH; PYTHIUM-PORPHYRAE; ECTOCARPUS-SILICULOSUS; INDUCED RESISTANCE; DEFENSE RESPONSES; OXIDATIVE BURST; GENE FAMILY; ARIAKE SEA; INFECTION; PLANTS	yyyy Pyropia yezoensis, one of the most economically important marine algae, suffers from the biotic stress of the oomycete necrotrophic pathogen Pythium porphyrae. However, little is known about the molecular defensive mechanisms employed by Pyr. yezoensis during the infection process. In the present study, we defined three stages of red rot disease based on histopathological features and photosynthetic physiology. Transcriptomic analysis was carried out at different stages of infection to identify the genes related to the innate immune system in Pyr. yezoensis. In total, 2139 up-regulated genes and 1672 down-regulated genes were identified from all the infected groups. Pathogen receptor genes, including three lectin genes (pattern recognition receptors (PRRs)) and five genes encoding typical plant R protein domains (leucine rich repeat (LRR), nucleotide binding site (NBS), or Toll/interleukin-1 receptor (TIR)), were found to be up-regulated after infection. Several defense mechanisms that were typically regarded as PAMP-triggered immunity (PTI) in plants were induced during the infection. These included defensive and protective enzymes, heat shock proteins, secondary metabolites, cellulase, and protease inhibitors. As a part of the effector-triggered immunity (ETI), the expression of genes related to the ubiquitin-proteasome system (UPS) and hypersensitive cell death response (HR) increased significantly during the infection. The current study suggests that, similar to plants, Pyr. yezoensis possesses a conserved innate immune system that counters the invasion of necrotrophic pathogen Pyt. porphyrae. However, the innate immunity genes of Pyr. yezoensis appear to be more ancient in origin compared to those in higher plants.	[Tang, Lei; Qiu, Liping; Liu, Cong; Du, Guoying; Tang, Xianghai; Mao, Yunxiang] Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China; [Mo, Zhaolan] Chinese Acad Fishery Sci, Key Lab Maricultural Organism Dis Control, Minist Agr & Rural Affairs, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China; [Mao, Yunxiang] Hainan Trop Ocean Univ, Key Lab Utilizat & Conservat Trop Marine Bioresou, Minist Educ, Coll Fisheries & Life Sci, Sanya 572022, Peoples R China	Mao, YX (corresponding author), Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China.; Mo, ZL (corresponding author), Chinese Acad Fishery Sci, Key Lab Maricultural Organism Dis Control, Minist Agr & Rural Affairs, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China.; Mao, YX (corresponding author), Hainan Trop Ocean Univ, Key Lab Utilizat & Conservat Trop Marine Bioresou, Minist Educ, Coll Fisheries & Life Sci, Sanya 572022, Peoples R China.	tangleiouc@163.com; qiuliping525@163.com; haiyangliucong@163.com; duguo923@ouc.edu.cn; mozl@ysfri.ac.cn; Txianghai@ouc.edu.cn; yxmao@ouc.edu.cn			National Key R&D Program of China [2018YFD0900106, 2018YFC1406700]; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018SDKJ0302-4]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [201762016]; MOA Modern Agricultural Talents Support Project	This work was financially supported by the National Key R&D Program of China (2018YFD0900106, 2018YFC1406700), the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (No. 2018SDKJ0302-4), the Fundamental Research Funds for the Central Universities (201762016), the MOA Modern Agricultural Talents Support Project.	Addepalli MK, 2002, FEMS MICROBIOL LETT, V211, P253, DOI 10.1111/j.1574-6968.2002.tb11233.x; Anand A, 2003, J EXP BOT, V54, P1101, DOI 10.1093/jxb/erg110; Arasaki S, 1947, NIPPON SUISAN GAKK, V13, P74, DOI [10.2331/suisan.13.74, DOI 10.2331/SUISAN.13.74]; Ausubel FM, 2005, NAT IMMUNOL, V6, P973, DOI 10.1038/ni1253; Bagnaresi P, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0051609; Berthonneau E, 2000, FEBS LETT, V470, P300, DOI 10.1016/S0014-5793(00)01343-0; Bhattacharya D, 2004, BIOESSAYS, V26, P50, DOI 10.1002/bies.10376; Bieri S, 2004, PLANT CELL, V16, P3480, DOI 10.1105/tpc.104.026682; Brawley SH, 2017, P NATL ACAD SCI USA, V114, pE6361, DOI 10.1073/pnas.1703088114; Bressendorff S, 2016, PLANT CELL, V28, P1328, DOI 10.1105/tpc.15.00774; Chen QZ, 2015, J PROTEOMICS, V115, P117, DOI 10.1016/j.jprot.2014.12.008; Coll NS, 2014, CELL DEATH DIFFER, V21, P1399, DOI 10.1038/cdd.2014.50; Coll NS, 2011, CELL DEATH DIFFER, V18, P1247, DOI 10.1038/cdd.2011.37; COOK RJ, 1980, PLANT DIS, V64, P102, DOI 10.1094/PD-64-1061; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; Craig A, 2009, J EXP BOT, V60, P1123, DOI 10.1093/jxb/erp059; de Vries Sophie, 2018, Commun Integr Biol, V11, P1, DOI 10.1080/19420889.2018.1486168; Diehl N, 2017, ALGAE-SEOUL, V32, P29, DOI 10.4490/algae.2017.32.2.25; Ding B, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00675; Ding HY, 2005, J APPL PHYCOL, V17, P51, DOI 10.1007/s10811-005-5523-6; Dowen RH, 2012, P NATL ACAD SCI USA, V109, pE2183, DOI 10.1073/pnas.1209329109; Duan KX, 2018, MOL PLANT MICROBE IN, V31, P445, DOI 10.1094/MPMI-10-17-0249-R; Dumilag RV, 2019, J GEN PLANT PATHOL, V85, P72, DOI 10.1007/s10327-018-0815-2; Ellis J, 2000, CURR OPIN PLANT BIOL, V3, P278, DOI 10.1016/S1369-5266(00)00080-7; Emanuelsson O, 2007, NAT PROTOC, V2, P953, DOI 10.1038/nprot.2007.131; Fagundes D, 2015, FUNCT INTEGR GENOMIC, V15, P639, DOI 10.1007/s10142-015-0459-7; FREI E, 1964, PROC R SOC SER B-BIO, V160, P314, DOI 10.1098/rspb.1964.0042; FUJITA Y, 1977, B JPN SOC SCI FISH, V43, P89; Gao YX, 2018, PLANT PHYSIOL, V177, P82, DOI 10.1104/pp.18.00185; Goldberg AL, 2003, NATURE, V426, P895, DOI 10.1038/nature02263; Goodman RN, 1994, HYPERSENSITIVE REACT; Goulitquer S, 2009, CHEMBIOCHEM, V10, P977, DOI 10.1002/cbic.200900004; Grenville-Briggs L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0024500; Grison R, 1996, NAT BIOTECHNOL, V14, P643, DOI 10.1038/nbt0596-643; Guan XY, 2013, J APPL PHYCOL, V25, P1341, DOI 10.1007/s10811-013-9976-8; Gururani MA, 2012, PHYSIOL MOL PLANT P, V78, P51, DOI 10.1016/j.pmpp.2012.01.002; Holeski LM, 2012, TRENDS ECOL EVOL, V27, P618, DOI 10.1016/j.tree.2012.07.011; Im SH, 2019, J PHYCOL, V55, P801, DOI 10.1111/jpy.12857; Iwai T, 2007, PLANT CELL PHYSIOL, V48, P915, DOI 10.1093/pcp/pcm062; Jones DA, 2004, CURR OPIN IMMUNOL, V16, P48, DOI 10.1016/j.coi.2003.11.016; Jones JDG, 2006, NATURE, V444, P323, DOI 10.1038/nature05286; Jorgensen HJL, 1998, PHYTOPATHOLOGY, V88, P698, DOI 10.1094/PHYTO.1998.88.7.698; Kawamura Y, 2005, PLANT DIS, V89, P1041, DOI 10.1094/PD-89-1041; Khan S, 2018, BMC GENOMICS, V19, DOI 10.1186/s12864-018-5229-1; Kim GH, 2014, ALGAE-SEOUL, V29, P249, DOI 10.4490/algae.2014.29.4.249; Kim JY, 2009, INT J MOL SCI, V10, P2860, DOI 10.3390/ijms10062860; Klochkova TA, 2016, J APPL PHYCOL, V28, P73, DOI 10.1007/s10811-015-0595-4; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; Laluk K, 2011, PLANT J, V68, P480, DOI 10.1111/j.1365-313X.2011.04702.x; Li XD, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-17140-4; Li YL, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-17059-w; [刘成圣 Liu Chengsheng], 2002, [海洋科学, Marine Sciences], V26, P44; Ma X, 2018, MOL PLANT MICROBE IN, V31, P420, DOI [10.1094/mpmi-10-17-0245-r, 10.1094/MPMI-10-17-0245-R]; Mazzucotelli E, 2006, CURR GENOMICS, V7, P509, DOI 10.2174/138920206779315728; Miraeiz E, 2020, THEOR APPL GENET, V133, P87, DOI 10.1007/s00122-019-03442-w; Mo ZL, 2016, J APPL PHYCOL, V28, P395, DOI 10.1007/s10811-015-0539-z; Mosca R, 2014, NUCLEIC ACIDS RES, V42, pD374, DOI 10.1093/nar/gkt887; MUKAI LS, 1981, J PHYCOL, V17, P192, DOI 10.1111/j.0022-3646.1981.00192.x; Nanda AK, 2010, J INTEGR PLANT BIOL, V52, P195, DOI 10.1111/j.1744-7909.2010.00933.x; Nandety RS, 2013, PLANT PHYSIOL, V162, P1459, DOI 10.1104/pp.113.219162; Nekrasov V, 2006, FEBS LETT, V580, P4236, DOI 10.1016/j.febslet.2006.06.077; Nielsen H, 2017, METHODS MOL BIOL, V1611, P59, DOI 10.1007/978-1-4939-7015-5_6; Nurnberger T, 2005, MOL PLANT PATHOL, V6, P335, DOI 10.1111/j.1364-3703.2005.00279.x; Park CS, 2014, J APPL PHYCOL, V26, P811, DOI 10.1007/s10811-013-0183-4; Pekkarinen AI, 2007, J AGR FOOD CHEM, V55, P2736, DOI 10.1021/jf0631777; Perez-Bueno ML, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.01790; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; Pieterse CMJ, 2012, PLANT PHYSIOL, V158, P545, DOI 10.1104/pp.112.900430; Pineda M, 2017, FUNCT PLANT BIOL, V44, P563, DOI [10.1071/FP16164, 10.1071/fp16164]; Provasoli L., 1968, P US JAP C HAK JAP 1; Qiu LP, 2019, J OCEANOL LIMNOL, V37, P1102, DOI 10.1007/s00343-019-8075-3; Sanchez AL, 2016, PLANT J, V88, P361, DOI 10.1111/tpj.13252; Shao ZQ, 2019, TRENDS PLANT SCI, V24, P9, DOI 10.1016/j.tplants.2018.10.015; Strittmatter M, 2016, PLANT CELL ENVIRON, V39, P259, DOI 10.1111/pce.12533; SUTTLE CA, 1990, NATURE, V347, P467, DOI 10.1038/347467a0; TAKEBE I, 1969, P NATL ACAD SCI USA, V64, P843, DOI 10.1073/pnas.64.3.843; Tsirigoti A, 2014, PLANT BIOLOGY, V16, P272, DOI 10.1111/plb.12041; Tsirigoti A, 2015, PROTOPLASMA, V252, P845, DOI 10.1007/s00709-014-0721-1; Le TN, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0458-3; Vaid N, 2013, MOL PLANT, V6, P1405, DOI 10.1093/mp/sst033; Vairappan CS, 2010, J APPL PHYCOL, V22, P305, DOI 10.1007/s10811-009-9460-7; van Bentem SDF, 2005, PLANT J, V43, P284, DOI 10.1111/j.1365-313X.2005.02450.x; Vierstra RD, 2009, NAT REV MOL CELL BIO, V10, P385, DOI 10.1038/nrm2688; Wang G, 2008, J APPL PHYCOL, V20, P403, DOI 10.1007/s10811-007-9274-4; Wang SS, 2013, J APPL PHYCOL, V25, P1215, DOI 10.1007/s10811-012-9911-4; Wei T, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0059720; Xia ST, 2013, PLANT PHYSIOL, V162, P1694, DOI 10.1104/pp.113.214551; Xiao XO, 2017, J PHYTOPATHOL, V165, P652, DOI 10.1111/jph.12604; Yang W, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0146910; Ye XY, 2001, BIOCHEM BIOPH RES CO, V289, P91, DOI 10.1006/bbrc.2001.5965; Zhu QL, 2018, SCI HORTIC-AMSTERDAM, V227, P169, DOI 10.1016/j.scienta.2017.09.033; Zipfel C, 2008, CURR OPIN IMMUNOL, V20, P10, DOI 10.1016/j.coi.2007.11.003; Zuluaga AP, 2016, MOL PLANT PATHOL, V17, P42, DOI 10.1111/mpp.12260	94	2	2	2	7	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		1422-0067		INT J MOL SCI	Int. J. Mol. Sci.	DEC	2019	20	23							5970	10.3390/ijms20235970			20	Biochemistry & Molecular Biology; Chemistry, Multidisciplinary	Biochemistry & Molecular Biology; Chemistry	JY5AR	WOS:000504428300152	31783543	DOAJ Gold, Green Published			2021-04-07	
J	Mignerot, L; Nagasato, C; Peters, AF; Perrineau, MM; Scornet, D; Pontheaux, F; Djema, W; Badis, Y; Motomura, T; Coelho, SM; Cock, JM				Mignerot, Laure; Nagasato, Chikako; Peters, Akira F.; Perrineau, Marie-Mathilde; Scornet, Delphine; Pontheaux, Florian; Djema, Walid; Badis, Yacine; Motomura, Taizo; Coelho, Susana M.; Cock, J. Mark			Unusual Patterns of Mitochondrial Inheritance in the Brown Alga Ectocarpus	MOLECULAR BIOLOGY AND EVOLUTION			English	Article						brown algae; Ectocarpus; life cycle; uniparental inheritance; mitochondria; parthenogenesis; recombination	MATERNAL INHERITANCE; DNA; CHLOROPLAST; EVOLUTION; GENOME; FERTILIZATION; PHAEOPHYCEAE; MECHANISMS; EXPRESSION; ORIGINS	Most eukaryotes inherit their mitochondria from only one of their parents. When there are different sexes, it is almost always the maternal mitochondria that are transmitted. Indeed, maternal uniparental inheritance has been reported for the brown alga Ectocarpus but we show in this study that different strains of Ectocarpus can exhibit different patterns of inheritance: Ectocarpus siliculosus strains showed maternal uniparental inheritance, as expected, but crosses using different Ectocarpus species 7 strains exhibited either paternal uniparental inheritance or an unusual pattern of transmission where progeny inherited either maternal or paternal mitochondria, but not both. A possible correlation between the pattern of mitochondrial inheritance and male gamete parthenogenesis was investigated. Moreover, in contrast to observations in the green lineage, we did not detect any change in the pattern of mitochondrial inheritance in mutant strains affected in life cycle progression. Finally, an analysis of field-isolated strains provided evidence of mitochondrial genome recombination in both Ectocarpus species.	[Mignerot, Laure; Perrineau, Marie-Mathilde; Scornet, Delphine; Pontheaux, Florian; Badis, Yacine; Coelho, Susana M.; Cock, J. Mark] Sorbonne Univ, UMR 8227 Integrat Biol Marine Models LBI2M, Algal Genet Grp, SBR,CNRS, Roscoff, France; [Nagasato, Chikako; Motomura, Taizo] Hokkaido Univ, Muroran Marine Stn, Sapporo, Hokkaido, Japan; [Peters, Akira F.] Bezhin Rosko, Santec, France; [Perrineau, Marie-Mathilde; Badis, Yacine] Scottish Assoc Marine Sci, Scottish Marine Inst, Oban, Argyll, Scotland; [Djema, Walid] Cote Azur Univ, Inria Sophia Antipolis, Bicore Team, Sophia Antipolis, France; [Djema, Walid] Cote Azur Univ, Inria Sophia Antipolis, McTAO Team, Sophia Antipolis, France	Cock, JM (corresponding author), Sorbonne Univ, UMR 8227 Integrat Biol Marine Models LBI2M, Algal Genet Grp, SBR,CNRS, Roscoff, France.	cock@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020; Perrineau, Marie-Mathilde/J-4459-2014	Perrineau, Marie-Mathilde/0000-0002-1772-7009; Cock, J. Mark/0000-0002-2650-0383; Badis, Yacine/0000-0003-1606-3906	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Japan Society for Promotion of Science (JSPS)Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science; Agence Nationale de la Recherche (project Bicycle)French National Research Agency (ANR) [ANR-10-BLAN-1727]; European Research Council (SexSea grant) [638240]; Sorbonne Universite	We thank Lucie Caradec for technical assistance. This work was supported by the Centre National de la Recherche Scientifique, the Japan Society for Promotion of Science (JSPS), the Agence Nationale de la Recherche (project Bicycle ANR-10-BLAN-1727), the European Research Council (SexSea grant agreement 638240), and Sorbonne Universite.	Abeel T, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gkr995; Allen JF, 1996, J THEOR BIOL, V180, P135, DOI 10.1006/jtbi.1996.0089; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Arun A, 2019, ELIFE, V8, DOI 10.7554/eLife.43101; Birky CW, 2001, ANNU REV GENET, V35, P125, DOI 10.1146/annurev.genet.35.102401.090231; Breton S, 2015, GENOME, V58, P423, DOI 10.1139/gen-2015-0090; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P262, DOI 10.1101/pdb.prot067942; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Crow JF, 2000, NAT REV GENET, V1, P40, DOI 10.1038/35049558; Doublet V, 2012, GENOME, V55, P234, DOI [10.1139/G2012-008, 10.1139/g2012-008]; FAURE S, 1994, CURR GENET, V25, P265, DOI 10.1007/BF00357172; Greiner S, 2015, BIOESSAYS, V37, P80, DOI 10.1002/bies.201400110; Han JW, 2014, PLANTA, V240, P1253, DOI 10.1007/s00425-014-2148-5; Havey MJ, 2004, J HERED, V95, P492, DOI 10.1093/jhered/esh081; Hoarau G, 2009, J PHYCOL, V45, P621, DOI 10.1111/j.1529-8817.2009.00679.x; Horst NA, 2016, NAT PLANTS, V2, DOI [10.1038/NPLANTS.2015.209, 10.1038/nplants.2015.209]; Kimura K, 2010, J PHYCOL, V46, P143, DOI 10.1111/j.1529-8817.2009.00779.x; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Lee JH, 2008, CELL, V133, P829, DOI 10.1016/j.cell.2008.04.028; Li H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Lohse M, 2013, NUCLEIC ACIDS RES, V41, pW575, DOI 10.1093/nar/gkt289; Lynch M, 1996, MOL BIOL EVOL, V13, P209, DOI 10.1093/oxfordjournals.molbev.a025557; Mignerot L, 2019, PLOS GENET, V15, DOI 10.1371/journal.pgen.1008211; Mishra P, 2014, NAT REV MOL CELL BIO, V15, P634, DOI 10.1038/nrm3877; Montecinos AE, 2017, J PHYCOL, V53, P17, DOI 10.1111/jpy.12452; MOTOMURA T, 1990, J PHYCOL, V26, P80, DOI 10.1111/j.0022-3646.1990.00080.x; Motomura T, 2010, J PLANT RES, V123, P185, DOI 10.1007/s10265-010-0313-x; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Nakamura S, 2010, J PLANT RES, V123, P163, DOI 10.1007/s10265-009-0295-8; NEALE DB, 1989, P NATL ACAD SCI USA, V86, P9347, DOI 10.1073/pnas.86.23.9347; Neff MM, 1998, PLANT J, V14, P387, DOI 10.1046/j.1365-313X.1998.00124.x; Nishimura Y, 2012, PLANT CELL, V24, P2401, DOI 10.1105/tpc.112.097865; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Roze D, 2005, GENETICS, V170, P1385, DOI 10.1534/genetics.104.039495; Sakakibara K, 2013, SCIENCE, V339, P1067, DOI 10.1126/science.1230082; Sato M, 2013, BBA-MOL CELL RES, V1833, P1979, DOI 10.1016/j.bbamcr.2013.03.010; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Takano H, 2010, J PLANT RES, V123, P131, DOI 10.1007/s10265-009-0268-y; Wilson A. J., 2012, Mycology - An International Journal on Fungal Biology, V3, P158; Zouros E, 2013, EVOL BIOL, V40, P1, DOI 10.1007/s11692-012-9195-2	45	1	1	0	4	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0737-4038	1537-1719		MOL BIOL EVOL	Mol. Biol. Evol.	DEC	2019	36	12					2778	2789		10.1093/molbev/msz186			12	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	JU5RY	WOS:000501735000010	31504759				2021-04-07	
J	Negre, D; Aite, M; Belcour, A; Frioux, C; Brillet-Gueguen, L; Liu, X; Bordron, P; Godfroy, O; Lipinska, AP; Leblanc, C; Siegel, A; Dittami, SM; Corre, E; Markov, GV				Negre, Delphine; Aite, Meziane; Belcour, Arnaud; Frioux, Clemence; Brillet-Gueguen, Loraine; Liu, Xi; Bordron, Philippe; Godfroy, Olivier; Lipinska, Agnieszka P.; Leblanc, Catherine; Siegel, Anne; Dittami, Simon M.; Corre, Erwan; Markov, Gabriel, V			Genome-Scale Metabolic Networks Shed Light on the Carotenoid Biosynthesis Pathway in the Brown Algae Saccharina japonica and Cladosiphon okamuranus	ANTIOXIDANTS			English	Article						genome-scale metabolic networks (GSMNs); data integration; brown algae; oxygenated carotenoid biosynthesis; fucoxanthin; abscisic acid; Saccharina japonica; Cladosiphon okamuranus	ABSCISIC-ACID BIOSYNTHESIS; LAMINARIA-JAPONICA; MOLYBDENUM COFACTOR; XANTHINE-DEHYDROGENASE; NEOXANTHIN SYNTHASE; METACYC DATABASE; ABA BIOSYNTHESIS; ALDEHYDE OXIDASE; FATTY-ACIDS; IDENTIFICATION	Understanding growth mechanisms in brown algae is a current scientific and economic challenge that can benefit from the modeling of their metabolic networks. The sequencing of the genomes of Saccharina japonica and Cladosiphon okamuranus has provided the necessary data for the reconstruction of Genome-Scale Metabolic Networks (GSMNs). The same in silico method deployed for the GSMN reconstruction of Ectocarpus siliculosus to investigate the metabolic capabilities of these two algae, was used. Integrating metabolic profiling data from the literature, we provided functional GSMNs composed of an average of 2230 metabolites and 3370 reactions. Based on these GSMNs and previously published work, we propose a model for the biosynthetic pathways of the main carotenoids in these two algae. We highlight, on the one hand, the reactions and enzymes that have been preserved through evolution and, on the other hand, the specificities related to brown algae. Our data further indicate that, if abscisic acid is produced by Saccharina japonica, its biosynthesis pathway seems to be different in its final steps from that described in land plants. Thus, our work illustrates the potential of GSMNs reconstructions for formalizing hypotheses that can be further tested using targeted biochemical approaches.	[Negre, Delphine; Brillet-Gueguen, Loraine; Godfroy, Olivier; Lipinska, Agnieszka P.; Leblanc, Catherine; Dittami, Simon M.; Markov, Gabriel, V] Sorbonne Univ, Stn Biol Roscoff SBR, Integrat Biol Marine Models LBI2M, CNRS, F-29680 Roscoff, France; [Negre, Delphine; Brillet-Gueguen, Loraine; Liu, Xi; Bordron, Philippe; Corre, Erwan] Sorbonne Univ, Stn Biol Roscoff, Plateforme ABiMS FR2424, CNRS, F-29680 Roscoff, France; [Negre, Delphine] Univ Nantes, UFR Sci Pharmaceut & Biol, Grp Mer Mol, Sante EA 2160, 9 Rue Bias, F-44035 Nantes, France; [Aite, Meziane; Belcour, Arnaud; Frioux, Clemence; Siegel, Anne] Univ Rennes 1, Inst Res IT & Random Syst IRISA, Equipe Dyliss, F-35052 Rennes, France; [Frioux, Clemence] Quadram Inst, Colney Lane, Norwich NR4 7UQ, Norfolk, England	Markov, GV (corresponding author), Sorbonne Univ, Stn Biol Roscoff SBR, Integrat Biol Marine Models LBI2M, CNRS, F-29680 Roscoff, France.	delphine.negre@sb-roscoff.fr; meziane.aite@inria.fr; arnaud.belcour@irisa.fr; clemence.frioux@quadram.ac.uk; loraine.gueguen@sb-roscoff.fr; xi.liu@sb-roscoff.fr; philippe.bordron@univ-nantes.fr; olivier.godfroy@sb-roscoff.fr; alipinska@sb-roscoff.fr; catherine.leblanc@sb-roscoff.fr; anne.siegel@irisa.fr; simon.dittami@sb-roscoff.fr; erwan.corre@sb-roscoff.fr; gabriel.markov@sb-roscoff.fr	Frioux, Clemence/A-1517-2019; corre, erwan/O-4669-2019	Frioux, Clemence/0000-0003-2114-0697; corre, erwan/0000-0001-6354-2278; Markov, Gabriel V./0000-0002-8566-7482; Belcour, Arnaud/0000-0003-1170-0785	French Government via the National Research Agency investment expenditure program IDEALG [ANR-10-BTBR-04]; Region Bretagne via the grant "SAD 2016 - METALG" [9673]	This research received funding from the French Government via the National Research Agency investment expenditure program IDEALG (ANR-10-BTBR-04) and from Region Bretagne via the grant "SAD 2016 - METALG (9673)".	Ahrazem O, 2016, INT J MOL SCI, V17, DOI 10.3390/ijms17111781; Aite M, 2018, PLOS COMPUT BIOL, V14, DOI 10.1371/journal.pcbi.1006146; Al-Babili S, 2000, FEBS LETT, V485, P168, DOI 10.1016/S0014-5793(00)02193-1; Alvarez R, 2014, CHEM REV, V114, P1, DOI 10.1021/cr400126u; Amengual J, 2019, NUTRIENTS, V11, DOI 10.3390/nu11102388; Ashburner M, 2000, NAT GENET, V25, P25, DOI 10.1038/75556; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Belcour A., INFERRING BIOCH REAC, DOI [10.1101/462556, DOI 10.1101/462556]; Bittner F, 2001, J BIOL CHEM, V276, P40381, DOI 10.1074/jbc.C100472200; Bleakley S, 2017, FOODS, V6, DOI 10.3390/foods6050033; Bohn T, 2019, ANTIOXIDANTS-BASEL, V8, DOI 10.3390/antiox8060179; Bouvier F, 2000, EUR J BIOCHEM, V267, P6346, DOI 10.1046/j.1432-1327.2000.01722.x; Bryant DM, 2017, CELL REP, V18, P762, DOI 10.1016/j.celrep.2016.12.063; Buchfink B, 2015, NAT METHODS, V12, P59, DOI 10.1038/nmeth.3176; Carbon S, 2019, NUCLEIC ACIDS RES, V47, pD330, DOI 10.1093/nar/gky1055; Caspi R, 2008, NUCLEIC ACIDS RES, V36, pD623, DOI 10.1093/nar/gkm900; Caspi R, 2018, NUCLEIC ACIDS RES, V46, pD633, DOI 10.1093/nar/gkx935; Caspi R, 2013, FEMS MICROBIOL LETT, V345, P85, DOI 10.1111/1574-6968.12194; Cheng KC, 2016, PHYTOCHEM LETT, V18, P113, DOI 10.1016/j.phytol.2016.09.008; Chi S, 2018, CURR GENET, V64, P259, DOI 10.1007/s00294-017-0733-4; Christaki E, 2013, J SCI FOOD AGR, V93, P5, DOI 10.1002/jsfa.5902; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coesel S, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002896; Cui HL, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-457; Cui HL, 2011, PLANT MOL BIOL REP, V29, P1013, DOI 10.1007/s11105-011-0297-2; Cunningham FX, 1996, PLANT CELL, V8, P1613, DOI 10.1105/tpc.8.9.1613; Cunningham FX, 2001, P NATL ACAD SCI USA, V98, P2905, DOI 10.1073/pnas.051618398; Dal'Molin CGD, 2010, PLANT PHYSIOL, V152, P579, DOI 10.1104/pp.109.148817; Dambek M, 2012, J EXP BOT, V63, P5607, DOI 10.1093/jxb/ers211; Dittami SM, 2017, PEERJ, V5, DOI 10.7717/peerj.4073; DUAN D, 1995, BOT MAR, V38, P409, DOI 10.1515/botm.1995.38.1-6.409; Ebenhoh Oliver, 2004, Genome Inform, V15, P35; Ebrahim A, 2015, MOL SYST BIOL, V11, DOI 10.15252/msb.20156157; Ebrahim A, 2013, BMC SYST BIOL, V7, DOI 10.1186/1752-0509-7-74; Esteban R, 2015, ENVIRON EXP BOT, V119, P63, DOI 10.1016/j.envexpbot.2015.04.009; Filiz E, 2015, TURK J AGRIC FOR, V39, P786, DOI 10.3906/tar-1411-68; Finkelstein Ruth R, 2002, Arabidopsis Book, V1, pe0058, DOI 10.1199/tab.0058; Firn RD, 2009, J EXP BOT, V60, P719, DOI 10.1093/jxb/erp002; Frainay C, 2018, METABOLITES, V8, DOI 10.3390/metabo8030051; Frioux C, 2019, THEOR PRACT LOG PROG, V19, P83, DOI 10.1017/S1471068418000455; Frommolt R, 2008, MOL BIOL EVOL, V25, P2653, DOI 10.1093/molbev/msn206; Getachew P, 2015, BOT MAR, V58, P267, DOI 10.1515/bot-2015-0007; Gotz S, 2008, NUCLEIC ACIDS RES, V36, P3420, DOI 10.1093/nar/gkn176; Gouy M, 2010, MOL BIOL EVOL, V27, P221, DOI 10.1093/molbev/msp259; Groisillier A, 2014, J EXP BOT, V65, P559, DOI 10.1093/jxb/ert405; Harrison PJ, 2014, ARCH BIOCHEM BIOPHYS, V544, P105, DOI 10.1016/j.abb.2013.10.005; Harvey AL, 2015, NAT REV DRUG DISCOV, V14, P111, DOI 10.1038/nrd4510; HAUGAN JA, 1994, BIOCHEM SYST ECOL, V22, P31, DOI 10.1016/0305-1978(94)90112-0; Hauser F, 2011, CURR BIOL, V21, pR346, DOI 10.1016/j.cub.2011.03.015; Havaux M, 2014, PLANT J, V79, P597, DOI 10.1111/tpj.12386; Hille R, 2011, COORDIN CHEM REV, V255, P1179, DOI 10.1016/j.ccr.2010.11.034; Hirschberg J, 2001, CURR OPIN PLANT BIOL, V4, P210, DOI 10.1016/S1369-5266(00)00163-1; HONYA M, 1994, J APPL PHYCOL, V6, P25, DOI 10.1007/BF02185900; Hwang JaeHo, 2014, Journal of Aquaculture Research and Development, V5, P286; Jahns P, 2009, BBA-BIOENERGETICS, V1787, P3, DOI 10.1016/j.bbabio.2008.09.013; KAKISAWA H, 1988, PHYTOCHEMISTRY, V27, P731, DOI 10.1016/0031-9422(88)84084-6; Kanazawa K, 2008, FOOD SCI TECHNOL RES, V14, P573, DOI 10.3136/fstr.14.573; Kanehisa M, 2000, NUCLEIC ACIDS RES, V28, P27, DOI 10.1093/nar/28.1.27; Karp Peter D, 2002, Bioinformatics, V18 Suppl 1, pS225; Kaufholdt D, 2017, FRONT PLANT SCI, V8, DOI 10.3389/fpls.2017.01946; King ZA, 2016, NUCLEIC ACIDS RES, V44, pD515, DOI 10.1093/nar/gkv1049; Kuczynska P, 2015, MAR DRUGS, V13, P5847, DOI 10.3390/md13095847; Lee KH, 2006, CELL, V126, P1109, DOI 10.1016/j.cell.2006.07.034; Li L, 2003, GENOME RES, V13, P2178, DOI 10.1101/gr.1224503; Lim SJ, 2019, FOOD CHEM, V272, P222, DOI 10.1016/j.foodchem.2018.08.034; Lohr M, 1999, P NATL ACAD SCI USA, V96, P8784, DOI 10.1073/pnas.96.15.8784; Loira N, 2017, BMC SYST BIOL, V11, DOI 10.1186/s12918-017-0441-1; Mackie A, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0075210; Mendel RR, 2013, J BIOL CHEM, V288, P13165, DOI 10.1074/jbc.R113.455311; Mendel RR, 2002, J EXP BOT, V53, P1689, DOI 10.1093/jxb/erf038; Mikami K, 2013, INT J MOL SCI, V14, P13763, DOI 10.3390/ijms140713763; MISE T., 2011, ADV J FOOD SCI TECHN, V3, P73; Moretti S, 2016, NUCLEIC ACIDS RES, V44, pD523, DOI 10.1093/nar/gkv1117; Moummou H, 2012, BMC PLANT BIOL, V12, DOI 10.1186/1471-2229-12-219; Mounien L, 2019, NUTRIENTS, V11, DOI 10.3390/nu11071562; Nambara E, 2005, ANNU REV PLANT BIOL, V56, P165, DOI 10.1146/annurev.arplant.56.032604.144046; Nimura K, 2002, J APPL PHYCOL, V14, P159, DOI 10.1023/A:1019929712633; Nishitsuji K, 2016, DNA RES, V23, P561, DOI 10.1093/dnares/dsw039; O'Brien KP, 2005, NUCLEIC ACIDS RES, V33, pD476, DOI 10.1093/nar/gki107; Pan S, 2018, CURR OPIN BIOTECH, V51, P103, DOI 10.1016/j.copbio.2017.12.012; Park JN, 2012, KOREAN J CHEM ENG, V29, P1604, DOI 10.1007/s11814-012-0051-y; PATTERSON GW, 1968, COMP BIOCHEM PHYSIOL, V24, P501, DOI 10.1016/0010-406X(68)91001-3; Peng T, 2018, BMC GENOMICS, V19, DOI 10.1186/s12864-018-5068-0; Potter SC, 2018, NUCLEIC ACIDS RES, V46, pW200, DOI 10.1093/nar/gky448; Prigent S, 2017, PLOS COMPUT BIOL, V13, DOI 10.1371/journal.pcbi.1005276; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; Priya R, 2014, GENE, V548, P223, DOI 10.1016/j.gene.2014.07.037; Rodriguez-Concepcion M, 2018, PROG LIPID RES, V70, P62, DOI 10.1016/j.plipres.2018.04.004; Rodriguez-Trelles F, 2003, P NATL ACAD SCI USA, V100, P13413, DOI 10.1073/pnas.1835646100; Saito H, 2010, J PHYCOL, V46, P665, DOI 10.1111/j.1529-8817.2010.00848.x; Sajilata MG, 2008, COMPR REV FOOD SCI F, V7, P29, DOI 10.1111/j.1541-4337.2007.00028.x; SALT SD, 1986, PHYSIOL MOL PLANT P, V28, P287, DOI 10.1016/S0048-4059(86)80071-6; Sandmann G, 2019, ANTIOXIDANTS-BASEL, V8, DOI 10.3390/antiox8070219; SCHAFFELKE B, 1995, J PLANT PHYSIOL, V146, P453, DOI 10.1016/S0176-1617(11)82008-0; Schwartz SH, 2003, PLANT PHYSIOL, V131, P1591, DOI 10.1104/pp.102.017921; Seo M, 2004, PLANT CELL PHYSIOL, V45, P1694, DOI 10.1093/pcp/pch198; Seo M, 2002, TRENDS PLANT SCI, V7, P41, DOI 10.1016/S1360-1385(01)02187-2; Shimizu K, 2018, J APPL PHYCOL, V30, P1371, DOI 10.1007/s10811-017-1320-2; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Sui XW, 2013, ARCH BIOCHEM BIOPHYS, V539, P203, DOI 10.1016/j.abb.2013.06.012; Takaichi S, 2011, MAR DRUGS, V9, P1101, DOI 10.3390/md9061101; Tako M, 2000, BOT MAR, V43, P393, DOI 10.1515/BOT.2000.040; Tan BC, 2003, PLANT J, V35, P44, DOI 10.1046/j.1365-313X.2003.01786.x; Teng LH, 2019, PLANTA, V249, P647, DOI 10.1007/s00425-018-3028-1; Thiele I, 2010, NAT PROTOC, V5, P93, DOI 10.1038/nprot.2009.203; Walter MH, 2011, NAT PROD REP, V28, P663, DOI 10.1039/c0np00036a; Wang SS, 2018, ACTA OCEANOL SIN, V37, P89, DOI 10.1007/s13131-018-1178-4; Wang XJ, 2014, BIOMED CHROMATOGR, V28, P275, DOI 10.1002/bmc.3018; Xie C, 2011, NUCLEIC ACIDS RES, V39, pW316, DOI 10.1093/nar/gkr483; Xiong LM, 2003, PLANT PHYSIOL, V133, P29, DOI 10.1104/pp.103.025395; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zhang PY, 2016, BMC PLANT BIOL, V16, DOI 10.1186/s12870-016-0750-3; Zhao LS, 2013, ANNU REV BIOCHEM, V82, P497, DOI 10.1146/annurev-biochem-052010-100934	113	2	2	3	10	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		2076-3921		ANTIOXIDANTS-BASEL	Antioxidants	NOV	2019	8	11							564	10.3390/antiox8110564			23	Biochemistry & Molecular Biology; Chemistry, Medicinal; Food Science & Technology	Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Food Science & Technology	JV3LS	WOS:000502268400060	31744163	DOAJ Gold, Green Published			2021-04-07	
J	Rabille, H; Torode, TA; Tesson, B; Le Bail, A; Billoud, B; Rolland, E; Le Panse, S; Jam, M; Charrier, B				Rabille, Herve; Torode, Thomas A.; Tesson, Benoit; Le Bail, Aude; Billoud, Bernard; Rolland, Elodie; Le Panse, Sophie; Jam, Murielle; Charrier, Benedicte			Alginates along the filament of the brown alga Ectocarpus help cells cope with stress	SCIENTIFIC REPORTS			English	Article							POLYMANNURONIC ACID 5-EPIMERASE; SILICULOSUS ECTOCARPALES; EXTRACELLULAR-MATRIX; PHENOLIC SUBSTANCES; PHYSICAL-PROPERTIES; WALL; FUCALES; PECTIN; POLYSACCHARIDES; PHAEOPHYCEAE	Ectocarpus is a filamentous brown alga, which cell wall is composed mainly of alginates and fucans (80%), two non-crystalline polysaccharide classes. Alginates are linear chains of epimers of 1,4-linked uronic acids, beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G). Previous physico-chemical studies showed that G-rich alginate gels are stiffer than M-rich alginate gels when prepared in vitro with calcium. In order to assess the possible role of alginates in Ectocarpus, we first immunolocalised M-rich or G-rich alginates using specific monoclonal antibodies along the filament. As a second step, we calculated the tensile stress experienced by the cell wall along the filament, and varied it with hypertonic or hypotonic solutions. As a third step, we measured the stiffness of the cell along the filament, using cell deformation measurements and atomic force microscopy. Overlapping of the three sets of data allowed to show that alginates co-localise with the stiffest and most stressed areas of the filament, namely the dome of the apical cell and the shanks of the central round cells. In addition, no major distinction between M-rich and G-rich alginate spatial patterns could be observed. Altogether, these results support that both M-rich and G-rich alginates play similar roles in stiffening the cell wall where the tensile stress is high and exposes cells to bursting, and that these roles are independent from cell growth and differentiation.	[Rabille, Herve; Le Bail, Aude; Billoud, Bernard; Rolland, Elodie; Charrier, Benedicte] Sorbonne Univ, Lab Biol Integrat Modeles Marins LBI2M, CNRS, Stn Biol, Roscoff, France; [Torode, Thomas A.] Univ Cambridge, Sainsbury Lab, Bateman St, Cambridge, England; [Tesson, Benoit] Univ Calif San Diego, Scripps Inst Oceanog, Marine Biol Res Div, La Jolla, CA 92093 USA; [Le Panse, Sophie] CNRS, Stn Biol, Platform Merimage, FR 2424, Roscoff, France; [Jam, Murielle] UPMC, CNRS, UMR8227, Marine Glycobiol Team,Stn Biol, Roscoff, France; [Le Bail, Aude] Friedrich Alexander Univ Erlangen Nurnberg, Dept Cell Biol, Erlangen, Germany	Charrier, B (corresponding author), Sorbonne Univ, Lab Biol Integrat Modeles Marins LBI2M, CNRS, Stn Biol, Roscoff, France.	benedicte.charrier@sb-roscoff.fr		Billoud, Bernard/0000-0002-5140-8087; Torode, Thomas/0000-0001-9717-6923; Charrier, Benedicte/0000-0001-5721-1640	Region Bretagne (ARED "Ectotip"); Sorbonne Universite	Herve Rabille was funded by Sorbonne Universite and Region Bretagne (ARED "Ectotip"). We thank Paul Knox and Cecile Herve for making the antibodies available. We are indebted to Mark Hildebrand for access to the AFM platform at Scripps Institute of Oceanography.	Arnold TM, 2003, OIKOS, V100, P406, DOI 10.1034/j.1600-0706.2003.11680.x; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Billoud B, 2008, FUNCT PLANT BIOL, V35, P1014, DOI 10.1071/FP08036; Bosch M, 2005, PLANT CELL, V17, P3219, DOI 10.1105/tpc.105.037473; Bosch M, 2005, PLANT PHYSIOL, V138, P1334, DOI 10.1104/pp.105.059865; Castle ES, 1937, J CELL COMPAR PHYSL, V10, P113, DOI 10.1002/jcp.1030100110; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Charrier B, 2019, TRENDS PLANT SCI, V24, P130, DOI 10.1016/j.tplants.2018.10.013; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Chebli Y, 2012, PLANT PHYSIOL, V160, P1940, DOI 10.1104/pp.112.199729; CHESHIRE AC, 1985, PHYCOLOGIA, V24, P147, DOI 10.2216/i0031-8884-24-2-147.1; Clarke JT, 2011, NEW PHYTOL, V192, P266, DOI 10.1111/j.1469-8137.2011.03794.x; CRAIGIE JS, 1984, CARBOHYD POLYM, V4, P237, DOI 10.1016/0144-8617(84)90001-8; Daher FB, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00523; Deniaud-Bouet E, 2017, CARBOHYD POLYM, V175, P395, DOI 10.1016/j.carbpol.2017.07.082; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Donati I, 2005, BIOMACROMOLECULES, V6, P1031, DOI 10.1021/bm049306e; DRAGET KI, 1994, CARBOHYD POLYM, V25, P31, DOI 10.1016/0144-8617(94)90159-7; Dumais J, 2006, INT J DEV BIOL, V50, P209, DOI 10.1387/ijdb.052066jd; Ertesvag H, 2015, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.00523; FORBES MA, 1979, BRIT PHYCOL J, V14, P69, DOI 10.1080/00071617900650101; Geitmann A, 2004, SEX PLANT REPROD, V17, P9, DOI 10.1007/s00497-004-0210-3; GRANT GT, 1973, FEBS LETT, V32, P195, DOI 10.1016/0014-5793(73)80770-7; GREEN PB, 1965, J CELL BIOL, V27, P343, DOI 10.1083/jcb.27.2.343; HAUG A, 1974, CARBOHYD RES, V32, P217, DOI 10.1016/S0008-6215(00)82100-X; HAUG A, 1969, BIOCHIM BIOPHYS ACTA, V192, P557, DOI 10.1016/0304-4165(69)90414-0; HEJNOWICZ Z, 1977, Z PFLANZENPHYSIOL, V81, P409, DOI 10.1016/S0044-328X(77)80176-1; Herve C, 2016, NEW PHYTOL, V209, P1428, DOI 10.1111/nph.13786; Inoue A, 2019, INT J ENGL LINGUIST, V9, P1, DOI 10.5539/ijel.v9n1p1; ISHIKAWA M, 1981, B JPN SOC SCI FISH, V47, P889; Jothisaraswathi S, 2006, J APPL PHYCOL, V18, P161, DOI 10.1007/s10811-006-9089-8; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Knoblauch J, 2016, PLANT CELL ENVIRON, V39, P1727, DOI 10.1111/pce.12736; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail Aude, 2013, Methods Mol Biol, V959, P323, DOI 10.1007/978-1-62703-221-6_22; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Linardic M, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-13767-5; Luder UH, 2004, PLANTA, V218, P928, DOI 10.1007/s00425-003-1176-3; Ma H, 2005, MICROBIOL-SGM, V151, P3679, DOI 10.1099/mic.0.28328-0; MADGWICK J, 1973, ACTA CHEM SCAND, V27, P3592, DOI 10.3891/acta.chem.scand.27-3592; MCKEE JWA, 1992, J APPL PHYCOL, V4, P357, DOI 10.1007/BF02185794; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Miller IJ, 1996, PHYTOCHEMISTRY, V41, P1315, DOI 10.1016/0031-9422(95)00741-5; Mine I, 2003, PLANTA, V217, P425, DOI 10.1007/s00425-003-0993-8; Mohnen D, 2008, CURR OPIN PLANT BIOL, V11, P266, DOI 10.1016/j.pbi.2008.03.006; Morch YA, 2008, BIOMACROMOLECULES, V9, P2360, DOI 10.1021/bm8003572; Nagasato C, 2010, PLANTA, V232, P287, DOI 10.1007/s00425-010-1188-8; Nezhad AS, 2013, J EXP BOT, V64, P4709, DOI 10.1093/jxb/ert254; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; Parre E, 2005, PLANTA, V220, P582, DOI 10.1007/s00425-004-1368-5; Ponce NMA, 2007, PHYCOLOGIA, V46, P675, DOI 10.2216/06-102.1; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; R Core Team, 2017, R LANG ENV STAT COMP; Rabille H, 2019, PLOS BIOL, V17, DOI 10.1371/journal.pbio.2005258; Raven JA, 2001, J EXP BOT, V52, P381, DOI 10.1093/jexbot/52.suppl_1.381; Salgado LT, 2009, J PHYCOL, V45, P193, DOI 10.1111/j.1529-8817.2008.00642.x; Schoenwaelder MEA, 1998, J PHYCOL, V34, P969, DOI 10.1046/j.1529-8817.1998.340969.x; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; SMIDSROD O, 1972, ACTA CHEM SCAND, V26, P2563, DOI 10.3891/acta.chem.scand.26-2563; SMIDSROD O, 1973, CARBOHYD RES, V27, P107, DOI 10.1016/S0008-6215(00)82430-1; Terauchi M, 2017, MAR GENOM, V32, P49, DOI 10.1016/j.margen.2016.12.002; Terauchi M, 2016, PLANTA, V244, P361, DOI 10.1007/s00425-016-2516-4; Tesson B, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00471; Thomas F, 2013, J BIOL CHEM, V288, P23021, DOI 10.1074/jbc.M113.467217; Tonon T, 2008, J PHYCOL, V44, P1250, DOI 10.1111/j.1529-8817.2008.00580.x; Torode T. A., 2018, PROTOCOLS MACROALGAE, V515; Torode TA, 2018, PLANT PHYSIOL, V176, P1547, DOI 10.1104/pp.17.01568; Torode TA, 2016, J EXP BOT, V67, P6089, DOI 10.1093/jxb/erw369; Torode TA, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0118366; TOVEY DJ, 1978, PHYCOLOGIA, V17, P17, DOI 10.2216/i0031-8884-17-1-17.1; Zerzour R, 2009, DEV BIOL, V334, P437, DOI 10.1016/j.ydbio.2009.07.044	72	5	5	3	6	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	2045-2322			SCI REP-UK	Sci Rep	SEP 10	2019	9								12956	10.1038/s41598-019-49427-z			17	Multidisciplinary Sciences	Science & Technology - Other Topics	IW4YK	WOS:000484985500004	31506545	DOAJ Gold, Green Published			2021-04-07	
J	Celis-Pla, PSM; Rodriguez-Rojas, F; Mendez, L; Moenne, F; Munoz, PT; Lobos, MG; Diaz, P; Sanchez-Lizaso, JL; Brown, MT; Moenne, A; Saez, CA				Celis-Pla, Paula S. M.; Rodriguez-Rojas, Fernanda; Mendez, Lorena; Moenne, Fabiola; Munoz, Pamela T.; Gabriela Lobos, M.; Diaz, Patricia; Luis Sanchez-Lizaso, Jose; Brown, Murray T.; Moenne, Alejandra; Saez, Claudio A.			MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Influence on Metal Exclusion/Extrusion Mechanisms and Photosynthesis	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES			English	Article						in vivo chlorophyll a florescence; physiology; mitogen activated protein kinases; Ulva compressa; metal accumulation	ACTIVATED PROTEIN-KINASE; ECTOCARPUS-SILICULOSUS; INTERTIDAL MACROALGAE; ENVIRONMENTAL-STRESS; DUNALIELLA-VIRIDIS; CROSS-TALK; RESPONSES; PHOSPHORYLATION; CALCIUM; ANTIOXIDANT	There is currently no information regarding the role that whole mitogen activated protein kinase (MAPK) pathways play in counteracting environmental stress in photosynthetic organisms. To address this gap, we exposed Ulva compressa to chronic levels of copper (10 mu M) specific inhibitors of Extracellular Signal Regulated Kinases (ERK), c-Jun N-terminal Kinases (JNK), and Cytokinin Specific Binding Protein (p38) MAPKs alone or in combination. Intracellular copper accumulation and photosynthetic activity (in vivo chlorophyll a fluorescence) were measured after 6 h, 24 h, 48 h, and 6 days of exposure. By day 6, when one (except JNK) or more of the MAPK pathways were inhibited under copper stress, there was a decrease in copper accumulation compared with algae exposed to copper alone. When at least two MAPKs were blocked, there was a decrease in photosynthetic activity expressed in lower productivity (ETRmax), efficiency (alpha(ETR)), and saturation of irradiance (EkETR), accompanied by higher non-photochemical quenching (NPQ(max)), compared to both the control and copper-only treatments. In terms of accumulation, once the MAPK pathways were partially or completely blocked under copper, there was crosstalk between these and other signaling mechanisms to enhance metal extrusion/exclusion from cells. Crosstalk occurred among MAPK pathways to maintain photosynthesis homeostasis, demonstrating the importance of the signaling pathways for physiological performance. This study is complemented by a parallel/complementary article Rodriguez-Rojas et al. on the role of MAPKs in copper-detoxification.	[Celis-Pla, Paula S. M.; Rodriguez-Rojas, Fernanda; Mendez, Lorena; Moenne, Fabiola; Munoz, Pamela T.; Saez, Claudio A.] Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar 2520000, Chile; [Munoz, Pamela T.] Univ Playa Ancha, Fac Ciencias Nat & Exactas, Ciencias Ambientales, Valparaiso 2340000, Chile; [Munoz, Pamela T.] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, Ciencias Mar & Biol Aplicada, E-03080 Alicante, Spain; [Gabriela Lobos, M.; Diaz, Patricia] Univ Valparaiso, Inst Quim & Bioquim, Lab Environm & Analyt Chem, Fac Ciencias, Valparaiso 234000, Chile; [Luis Sanchez-Lizaso, Jose] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, E-03080 Alicante, Spain; [Brown, Murray T.] Univ Plymouth, Sch Biol & Marine Sci, Plymouth PL4 8AA, Devon, England; [Moenne, Alejandra] Univ Santiago Chile, Fac Quim & Biol, Lab Marine Biotechnol, Santiago 9170020, Chile; [Saez, Claudio A.] Univ Playa Ancha, HUB AMBIENTAL UPLA, Valparaiso 2340000, Chile	Saez, CA (corresponding author), Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar 2520000, Chile.; Saez, CA (corresponding author), Univ Playa Ancha, HUB AMBIENTAL UPLA, Valparaiso 2340000, Chile.	paulacelispla@upla.cl; fernanda.rodriguez@upla.cl; lorena.mendez@alumnos.uv.cl; fabiola.moenne@upla.cl; pamela.munoz@upla.cl; gabriela.lobos@uv.cl; patriciaediazg@gmail.com; jl.sanchez@ua.es; mt.brown@plymouth.ac.uk; alejandra.moenne@usach.cl; claudio.saez@upla.cl	Lizaso, Jose Luis L Sanchez/J-4939-2017; Saez, Claudio/F-5978-2015	Lizaso, Jose Luis L Sanchez/0000-0002-3927-5699; Moenne, Alejandra/0000-0002-7309-9713; Saez, Claudio/0000-0002-5037-3484	 [11160369]	Project FONDECYT NO. 11160369 granted to C.A. Saez.	Aburai N, 2015, BIOTECHNOL LETT, V37, P1073, DOI 10.1007/s10529-015-1770-z; Anderson M, 2008, PERMANOVA PRIMER GUI, P218; Celis-Pla PSM, 2018, MAR POLLUT BULL, V128, P214, DOI 10.1016/j.marpolbul.2018.01.005; Celis-Pla PSM, 2016, MAR ENVIRON RES, V115, P89, DOI 10.1016/j.marenvres.2015.11.014; Celis-Pla PSM, 2014, SCI MAR, V78, P377, DOI 10.3989/scimar.04053.05A; Doan TKP, 2012, TISSUE ENG REGEN MED, V9, P283, DOI 10.1007/s13770-012-0352-6; EILERS PHC, 1988, ECOL MODEL, V42, P199, DOI 10.1016/0304-3800(88)90057-9; Ferreira JG, 2011, ESTUAR COAST SHELF S, V93, P117, DOI 10.1016/j.ecss.2011.03.014; Figueroa FL, 2003, PHOTOSYNTH RES, V75, P259, DOI 10.1023/A:1023936313544; Franchi N, 2013, J INVERTEBR PATHOL, V112, P260, DOI 10.1016/j.jip.2012.12.001; de Oliveira EAG, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0045707; Gao G, 2017, ENVIRON EXP BOT, V135, P63, DOI 10.1016/j.envexpbot.2016.12.007; Gasulla F, 2016, PLANT CELL PHYSIOL, V57, P1908, DOI 10.1093/pcp/pcw111; Gawronski P, 2014, MOL PLANT, V7, P1151, DOI 10.1093/mp/ssu060; Gomez M, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.00754; Gomez M, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00182; Gonzalez A, 2012, PLANT SIGNAL BEHAV, V7, P728, DOI 10.4161/psb.20355; Gonzalez A, 2012, PLANT PHYSIOL, V158, P1451, DOI 10.1104/pp.111.191759; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; GOWER JC, 1966, BIOMETRIKA, V53, P325, DOI 10.1093/biomet/53.3-4.325; Guyton KZ, 1996, J BIOL CHEM, V271, P4138, DOI 10.1074/jbc.271.7.3604; Jalmi SK, 2018, FRONT PLANT SCI, V9, DOI 10.3389/fpls.2018.00012; Jimenez C, 2004, BBA-MOL CELL RES, V1644, P61, DOI 10.1016/j.bbamcr.2003.10.009; Jimenez C, 2007, J EXP BOT, V58, P1001, DOI 10.1093/jxb/erl260; Kamata H, 2005, CELL, V120, P649, DOI 10.1016/j.cell.2004.12.041; Khokon MAR, 2015, PLANT BIOLOGY, V17, P946, DOI 10.1111/plb.12321; Kim DY, 2007, PLANT J, V50, P207, DOI 10.1111/j.1365-313X.2007.03044.x; Kumar K. Suresh, 2009, Toxicology and Environmental Health Sciences, V1, P17; Kungolos A, 2009, SCI TOTAL ENVIRON, V407, P4610, DOI 10.1016/j.scitotenv.2009.04.038; Laporte D, 2016, AQUAT TOXICOL, V177, P433, DOI 10.1016/j.aquatox.2016.06.017; Li SN, 2016, PLANT CELL, V28, P2866, DOI 10.1105/tpc.16.00130; Li YB, 2017, NEW PHYTOL, V215, P1102, DOI 10.1111/nph.14647; Livanos P, 2014, PLANT CELL ENVIRON, V37, P1130, DOI 10.1111/pce.12222; Ludwig AA, 2005, P NATL ACAD SCI USA, V102, P10736, DOI 10.1073/pnas.0502954102; Matsuzawa A, 2008, BBA-GEN SUBJECTS, V1780, P1325, DOI 10.1016/j.bbagen.2007.12.011; Mehlmer N, 2010, PLANT J, V63, P484, DOI 10.1111/j.1365-313X.2010.04257.x; Meng D, 2007, FREE RADICAL BIO MED, V42, P1651, DOI 10.1016/j.freeradbiomed.2007.01.037; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Charneco GO, 2018, ENVIRON EXP BOT, V156, P203, DOI 10.1016/j.envexpbot.2018.08.030; Parages ML, 2014, AQUAT BIOL, V22, P213, DOI 10.3354/ab00592; Parages ML, 2014, J PLANT PHYSIOL, V171, P276, DOI 10.1016/j.jplph.2013.08.005; Ratkevicius N, 2003, PLANT CELL ENVIRON, V26, P1599, DOI 10.1046/j.1365-3040.2003.01073.x; Rodriguez-Rojas F., 2019, INT J MOL SCI; Roelofs D, 2008, FUNCT ECOL, V22, P8, DOI 10.1111/j.1365-2435.2007.01312.x; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Saez CA, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0050170; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; Schreiber U., 1995, ECOPHYSIOLOGY PHOTOS, P49, DOI [DOI 10.1007/978-3-642-79354-7_3, 10.1007/978-3-642-79354-7_3]; Sheppard C., 2018, WORLD SEAS ENV EVALU; Silvan JM, 2016, FOOD FUNCT, V7, P1067, DOI [10.1039/C5FO01368B, 10.1039/c5fo01368b]; Simon DF, 2013, APPL ENVIRON MICROB, V79, P4774, DOI 10.1128/AEM.00998-13; Sinha AK, 2011, PLANT SIGNAL BEHAV, V6, P196, DOI 10.4161/psb.6.2.14701; Song Y., 2011, J SIGNAL TRANSDUCT, V2011; Su JB, 2018, PLOS BIOL, V16, DOI 10.1371/journal.pbio.2004122; Sytar O, 2013, ACTA PHYSIOL PLANT, V35, P985, DOI 10.1007/s11738-012-1169-6; Taylor E, 2018, FRONT ENDOCRINOL, V9, DOI 10.3389/fendo.2018.00439; Wang X, 2017, BIOL PLANTARUM, V61, P693, DOI [10.1007/s10535-017-0734-7, 10.1007/s10535-016-0697-0]; Xu HY, 2018, PLANT PHYSIOL, V178, P907, DOI 10.1104/pp.18.00903; Yruela I, 2009, FUNCT PLANT BIOL, V36, P409, DOI 10.1071/FP08288; Zhao HY, 2015, J VET SCI, V16, P297, DOI 10.4142/jvs.2015.16.3.297; ZHU D, 2014, ACTA SCI VET, V42, P8	63	7	7	1	3	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		1422-0067		INT J MOL SCI	Int. J. Mol. Sci.	SEP 2	2019	20	18							4547	10.3390/ijms20184547			16	Biochemistry & Molecular Biology; Chemistry, Multidisciplinary	Biochemistry & Molecular Biology; Chemistry	JC2IK	WOS:000489100500226	31540294	DOAJ Gold, Green Published			2021-04-07	
J	Rodriguez-Rojas, F; Celis-Pla, PSM; Mendez, L; Moenne, F; Munoz, PT; Lobos, MG; Diaz, P; Sanchez-Lizaso, JL; Brown, MT; Moenne, A; Saez, CA				Rodriguez-Rojas, Fernanda; Celis-Pla, Paula S. M.; Mendez, Lorena; Moenne, Fabiola; Munoz, Pamela T.; Gabriela Lobos, M.; Diaz, Patricia; Luis Sanchez-Lizaso, Jose; Brown, Murray T.; Moenne, Alejandra; Saez, Claudio A.			MAPK Pathway under Chronic Copper Excess in Green Macroalgae (Chlorophyta): Involvement in the Regulation of Detoxification Mechanisms	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES			English	Article						mitogen-activated protein kinases; Ulva compressa; antioxidant; oxidative stress; metal chelator	GENE-EXPRESSION; ECTOCARPUS-SILICULOSUS; ANTIOXIDANT RESPONSES; INTERTIDAL MACROALGAE; ACTIVATION; GLUTATHIONE; RESISTANCE; ASCORBATE; PHOSPHORYLATION; MELANOMA	Following the physiological complementary/parallel Celis-Pla et al., by inhibiting extracellular signal regulated kinases (ERK), c-Jun N-terminal kinases (JNK), and cytokinin specific binding protein (p38), we assessed the role of the mitogen-activated protein kinases (MAPK) pathway in detoxification responses mediated by chronic copper (10 mu M) in U. compressa. Parameters were taken at 6, 24, and 48 h, and 6 days (d). H2O2 and lipid peroxidation under copper and inhibition of ERK, JNK, or p38 alone increased but recovered by the sixth day. By blocking two or more MAPKs under copper, H2O2 and lipid peroxidation decayed even below controls. Inhibition of more than one MAPK (at 6 d) caused a decrease in total glutathione (reduced glutathione (GSH) + oxidised glutathione (GSSG)) and ascorbate (reduced ascorbate (ASC) + dehydroascorbate (DHA)), although in the latter it did not occur when the whole MAPK was blocked. Catalase (CAT), superoxide dismutase (SOD), thioredoxin (TRX) ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), and glutathione synthase (GS), were downregulated when blocking more than one MAPK pathway. When one MAPK pathway was blocked under copper, a recovery and even enhancement of detoxification mechanisms was observed, likely due to crosstalk within the MAPKs and/or other signalling processes. In contrast, when more than one MAPK pathway were blocked under copper, impairment of detoxification defences occurred, demonstrating that MAPKs were key signalling mechanisms for detoxification in macroalgae.	[Rodriguez-Rojas, Fernanda; Celis-Pla, Paula S. M.; Mendez, Lorena; Moenne, Fabiola; Munoz, Pamela T.; Saez, Claudio A.] Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar 2520000, Chile; [Rodriguez-Rojas, Fernanda; Celis-Pla, Paula S. M.; Moenne, Fabiola; Saez, Claudio A.] Univ Playa Ancha, HUB AMBIENTAL UPLA, Valparaiso 2340000, Chile; [Munoz, Pamela T.] Univ Playa Ancha, Fac Ciencias Nat & Exactas, Ciencias Ambientales, Valparaiso 2340000, Chile; [Munoz, Pamela T.] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, Ciencias Mar & Biol Aplicada, E-03080 Alicante, Spain; [Gabriela Lobos, M.; Diaz, Patricia] Univ Valparaiso, Fac Ciencias, Inst Quim & Bioquim, Lab Environm & Analyt Chem, Valparaiso 234000, Chile; [Luis Sanchez-Lizaso, Jose] Univ Alicante, Dept Ciencias Mar & Biol Aplicada, E-03080 Alicante, Spain; [Brown, Murray T.] Univ Plymouth, Sch Biol & Marine Sci, Plymouth PL4 8AA, Devon, England; [Moenne, Alejandra] Univ Santiago Chile, Fac Quim & Biol, Lab Marine Biotechnol, Santiago 9170020, Chile	Saez, CA (corresponding author), Univ Playa Ancha, Ctr Estudios Avanzados, Lab Aquat Environm Res, Vina Del Mar 2520000, Chile.; Saez, CA (corresponding author), Univ Playa Ancha, HUB AMBIENTAL UPLA, Valparaiso 2340000, Chile.	claudio.saez@upla.cl	Lizaso, Jose Luis L Sanchez/J-4939-2017; Saez, Claudio/F-5978-2015	Lizaso, Jose Luis L Sanchez/0000-0002-3927-5699; Saez, Claudio/0000-0002-5037-3484; Moenne, Alejandra/0000-0002-7309-9713	 [11160369]	Project FONDECYT NO. 11160369 granted to C.A. Saez	Abel EV, 2013, J CLIN INVEST, V123, P2155, DOI 10.1172/JCI65780; Ahanger MA, 2018, J PLANT GROWTH REGUL, V37, P1007, DOI 10.1007/s00344-018-9855-2; Anderson M, 2008, PERMANOVA PRIMER GUI, P218; Benzie IFF, 1999, METHOD ENZYMOL, V299, P15; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; Buchanan BB, 2017, PLANT CELL PHYSIOL, V58, P1826, DOI [10.1093/pcp/pcx119, 10.1093/p]; Burkhead JL, 2009, NEW PHYTOL, V182, P799, DOI 10.1111/j.1469-8137.2009.02846.x; Celis-Pla P.S.M., 2019, INT J MOL SCI; Cuypers A, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.00470; Davies MA, 2013, JNCI-J NATL CANCER I, V105, P9, DOI 10.1093/jnci/djs507; Foyer CH, 2016, PLANT CELL ENVIRON, V39, P951, DOI 10.1111/pce.12621; Foyer CH, 2011, PLANT PHYSIOL, V155, P2, DOI 10.1104/pp.110.167569; Gonzalez A, 2012, PLANT PHYSIOL, V158, P1451, DOI 10.1104/pp.111.191759; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; Gopal YNV, 2010, CANCER RES, V70, P8736, DOI 10.1158/0008-5472.CAN-10-0902; Ismail GA, 2017, ENVIRON MONIT ASSESS, V189, DOI 10.1007/s10661-017-5775-z; Jagodzik P, 2018, FRONT PLANT SCI, V9, DOI 10.3389/fpls.2018.01387; Janitza P, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00271; Jervis Les, 1997, Biochemical Society Transactions, V25, p63S; Junglee S., 2014, AM J ANAL CHEM, V5, P730, DOI DOI 10.4236/AJAC.2014.511081; Karuppanapandian T, 2013, ACTA PHYSIOL PLANT, V35, P2429, DOI 10.1007/s11738-013-1277-y; Laporte D, 2016, AQUAT TOXICOL, V177, P433, DOI 10.1016/j.aquatox.2016.06.017; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Mellado M, 2012, PLANT PHYSIOL BIOCH, V51, P102, DOI 10.1016/j.plaphy.2011.10.007; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Navarrete A, 2019, PLANT PHYSIOL BIOCH, V135, P423, DOI 10.1016/j.plaphy.2018.11.019; Nazarian R, 2010, NATURE, V468, P973, DOI 10.1038/nature09626; Newlaczyl AU, 2014, BIOCHEM SOC T, V42, P742, DOI 10.1042/BST20140057; Charneco GO, 2018, ENVIRON EXP BOT, V156, P203, DOI 10.1016/j.envexpbot.2018.08.030; Parages ML, 2014, AQUAT BIOL, V22, P213, DOI 10.3354/ab00592; Parages ML, 2014, J PLANT PHYSIOL, V171, P276, DOI 10.1016/j.jplph.2013.08.005; Paraiso KHT, 2011, CANCER RES, V71, P2750, DOI 10.1158/0008-5472.CAN-10-2954; Qi Q, 2019, PLANT PHYSIOL BIOCH, V135, P1, DOI 10.1016/j.plaphy.2018.11.017; Rahman I, 2006, NAT PROTOC, V1, P3159, DOI 10.1038/nprot.2006.378; Ratkevicius N, 2003, PLANT CELL ENVIRON, V26, P1599, DOI 10.1046/j.1365-3040.2003.01073.x; Rodriguez FE, 2018, BMC GENOMICS, V19, DOI 10.1186/s12864-018-5226-4; Saez CA, 2015, PHYCOLOGIA, V54, P425, DOI 10.2216/15-30.1; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Shan CJ, 2018, PROTOPLASMA, V255, P977, DOI 10.1007/s00709-017-1183-z; Sinha AK, 2011, PLANT SIGNAL BEHAV, V6, P196, DOI 10.4161/psb.6.2.14701; Song Y., 2011, J SIGNAL TRANSDUCT, V2011; Villanueva J, 2010, CANCER CELL, V18, P683, DOI 10.1016/j.ccr.2010.11.023; Wu TM, 2008, PHYCOLOGIA, V47, P346, DOI 10.2216/PH07-77.1; Yoo SJ, 2014, PLANT PATHOLOGY J, V30, P168, DOI 10.5423/PPJ.OA.10.2013.0106; Zhao FY, 2014, ACTA PHYSIOL PLANT, V36, P1879, DOI 10.1007/s11738-014-1564-2	46	7	7	0	2	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND		1422-0067		INT J MOL SCI	Int. J. Mol. Sci.	SEP 2	2019	20	18							4546	10.3390/ijms20184546			19	Biochemistry & Molecular Biology; Chemistry, Multidisciplinary	Biochemistry & Molecular Biology; Chemistry	JC2IK	WOS:000489100500225	31540290	DOAJ Gold, Green Published			2021-04-07	
J	Poliner, E; Cummings, C; Newton, L; Farre, EM				Poliner, Eric; Cummings, Cameron; Newton, Linsey; Farre, Eva M.			Identification of circadian rhythms in Nannochloropsis species using bioluminescence reporter lines	PLANT JOURNAL			English	Article						circadian rhythms; stramenopiles; luciferase; transcription; cell division; bioluminescence reporter; Nannochloropsis oceanica; Nannochloropsis salina; Nannochloropsis genus	REGULATED GENE-EXPRESSION; BLUE-LIGHT; CELL-DIVISION; CRYPTOCHROME/PHOTOLYASE FAMILY; PHAEODACTYLUM-TRICORNUTUM; ECTOCARPUS PHAEOPHYTA; FUNCTIONAL-ANALYSIS; OCEANICA CCMP1779; CONSTANT LIGHT; FAST RESPONSES	Circadian clocks allow organisms to predict environmental changes caused by the rotation of the Earth. Although circadian rhythms are widespread among different taxa, the core components of circadian oscillators are not conserved and differ between bacteria, plants, animals and fungi. Stramenopiles are a large group of organisms in which circadian rhythms have been only poorly characterized and no clock components have been identified. We have investigated cell division and molecular rhythms in Nannochloropsis species. In the four strains tested, cell division occurred principally during the night period under diel conditions; however, these rhythms damped within 2-3 days after transfer to constant light. We developed firefly luciferase reporters for the long-term monitoring of in vivo transcriptional rhythms in two Nannochlropsis species, Nannochloropsis oceanica CCMP1779 and Nannochloropsis salina CCMP537. The reporter lines express anticipatory behavior under light/dark cycles and free-running bioluminescence rhythms with periods of similar to 21-31 h that damped within similar to 3-4 days under constant light. Using different entrainment regimes, we demonstrate that these rhythms are modulated by a circadian-type oscillator. In addition, the phase of free-running luminescence rhythms can be modulated pharmacologically using a CK1 epsilon/delta inhibitor, suggesting a role of this kinase in the Nannochloropsis clock. Together with the molecular and genomic tools available for Nannochloropsis species, these reporter lines represent an excellent system for future studies on the molecular mechanisms of stramenopile circadian oscillators.	[Poliner, Eric; Farre, Eva M.] Michigan State Univ, Cell & Mol Biol Program, E Lansing, MI 48824 USA; [Cummings, Cameron; Newton, Linsey; Farre, Eva M.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA; [Cummings, Cameron] Adimab, Lebanon, NH USA	Farre, EM (corresponding author), Michigan State Univ, Cell & Mol Biol Program, E Lansing, MI 48824 USA.; Farre, EM (corresponding author), Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA.	farre@msu.edu		Farre, Eva/0000-0003-1566-7572	National Science FoundationNational Science Foundation (NSF) [IOS-1354721]; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [T32GM110523, T32GM110523, T32GM110523, T32GM110523] Funding Source: NIH RePORTER	We thank Hideki Takahashi for the anti-Renilla antibody, Christoph Benning for access to the cell counter, and Stephanie Taylor for discussions and advice on the analyses of cyclic expression. We also thank Traverse Cottrell for technical assistance. This work was funded by a grant from the National Science Foundation (IOS-1354721) to E.M.F.	ANDERSON RW, 1985, EXP CELL RES, V157, P144, DOI 10.1016/0014-4827(85)90158-2; Andreu N, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0010777; Aschoff J, 1999, JPN J PHYSIOL, V49, P11, DOI 10.2170/jjphysiol.49.11; Ashworth J, 2013, P NATL ACAD SCI USA, V110, P7518, DOI 10.1073/pnas.1300962110; Badura L, 2007, J PHARMACOL EXP THER, V322, P730, DOI 10.1124/jpet.107.122846; Banerjee A, 2016, NUCLEIC ACIDS RES, V44, P5957, DOI 10.1093/nar/gkw420; BARGIELLO TA, 1984, NATURE, V312, P752, DOI 10.1038/312752a0; Bell-Pedersen D, 2005, NAT REV GENET, V6, P544, DOI 10.1038/nrg1633; Biller SJ, 2018, MSYSTEMS, V3, DOI 10.1128/mSystems.00040-18; Bordyugov G, 2015, J R SOC INTERFACE, V12, DOI 10.1098/rsif.2015.0282; Braun R, 2014, J PHYCOL, V50, P515, DOI 10.1111/jpy.12177; BURG JP, 1972, GEOPHYSICS, V37, P375, DOI 10.1190/1.1440265; Burki F, 2014, CSH PERSPECT BIOL, V6, DOI 10.1101/cshperspect.a016147; Carpinelli EC, 2014, MOL PLANT, V7, P323, DOI 10.1093/mp/sst120; Chauton MS, 2013, PLANT PHYSIOL, V161, P1034, DOI 10.1104/pp.112.206177; Coesel S, 2009, EMBO REP, V10, P655, DOI 10.1038/embor.2009.59; Cohen SE, 2015, MICROBIOL MOL BIOL R, V79, P373, DOI 10.1128/MMBR.00036-15; Corellou F, 2009, PLANT CELL, V21, P3436, DOI 10.1105/tpc.109.068825; Crane BR, 2014, ANNU REV BIOCHEM, V83, P191, DOI 10.1146/annurev-biochem-060713-035644; Dodd AN, 2005, SCIENCE, V309, P630, DOI 10.1126/science.1115581; Dodd AN, 2004, NEW PHYTOL, V162, P63, DOI 10.1111/j.1469-8137.2004.01005.x; Doherty CJ, 2010, ANNU REV GENET, V44, P419, DOI 10.1146/annurev-genet-102209-163432; Dunlap JC, 2017, MICROBIOL SPECTR, V5, DOI 10.1128/microbiolspec.FUNK-0039-2016; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Eide EJ, 2005, MOL CELL BIOL, V25, P2795, DOI 10.1128/MCB.25.7.2795-2807.2005; England CG, 2016, BIOCONJUGATE CHEM, V27, P1175, DOI 10.1021/acs.bioconjchem.6b00112; ENRIGHT JT, 1965, J THEOR BIOL, V8, P426, DOI 10.1016/0022-5193(65)90021-4; Farre EM, 2007, PLANT J, V52, P548, DOI 10.1111/j.1365-313X.2007.03258.x; Farre EM, 2013, CURR OPIN PLANT BIOL, V16, P621, DOI 10.1016/j.pbi.2013.06.015; Ferrante P, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003200; Gehan MA, 2015, CURR OPIN PLANT BIOL, V24, P39, DOI 10.1016/j.pbi.2015.01.004; GOTO K, 1995, J CELL BIOL, V129, P1061, DOI 10.1083/jcb.129.4.1061; Goyaa ME, 2016, P NATL ACAD SCI USA, V113, pE7837, DOI 10.1073/pnas.1605769113; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Gyllenstrand N, 2014, PLANT CELL PHYSIOL, V55, P535, DOI 10.1093/pcp/pct199; Hall MP, 2012, ACS CHEM BIOL, V7, P1848, DOI 10.1021/cb3002478; Hardigan MA, 2017, P NATL ACAD SCI USA, V114, pE9999, DOI 10.1073/pnas.1714380114; Heijde M, 2010, PLANT CELL ENVIRON, V33, P1614, DOI 10.1111/j.1365-3040.2010.02168.x; Heintz U, 2016, ELIFE, V5, DOI 10.7554/eLife.11860; Herman E, 2013, BIOCHEMISTRY-US, V52, P3094, DOI 10.1021/bi400197u; Hevia MA, 2015, P NATL ACAD SCI USA, V112, P8744, DOI 10.1073/pnas.1508432112; Hisatomi O, 2013, PLANT CELL PHYSIOL, V54, P93, DOI 10.1093/pcp/pcs160; Holtzendorff J, 2008, J BIOL RHYTHM, V23, P187, DOI 10.1177/0748730408316040; Hong HY, 2000, ELECTROPHORESIS, V21, P841, DOI 10.1002/(SICI)1522-2683(20000301)21:5<841::AID-ELPS841>3.0.CO;2-4; Hurley JM, 2014, P NATL ACAD SCI USA, V111, P16995, DOI 10.1073/pnas.1418963111; Izumo M, 2006, PLOS COMPUT BIOL, V2, P1248, DOI 10.1371/journal.pcbi.0020136; Johnson CH, 2010, CELL CYCLE, V9, P3864, DOI 10.4161/cc.9.19.13205; Johnson CH, 2003, CHRONOBIOL INT, V20, P741, DOI 10.1081/CBI-120024211; Kilian O, 2011, P NATL ACAD SCI USA, V108, P21265, DOI 10.1073/pnas.1105861108; Kirk JT, 2011, LIGHT PHOTOSYNTHESIS; Kroth PG, 2017, J PLANT PHYSIOL, V217, P20, DOI 10.1016/j.jplph.2017.06.010; Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]; Kusakina J, 2014, PLANT CELL ENVIRON, V37, P327, DOI 10.1111/pce.12152; Lee SJ, 2018, MYCORRHIZA, V28, P523, DOI 10.1007/s00572-018-0843-y; Leitao JMM, 2010, J PHOTOCH PHOTOBIO B, V101, P1, DOI 10.1016/j.jphotobiol.2010.06.015; Linde AM, 2017, NEW PHYTOL, V216, P576, DOI 10.1111/nph.14487; Makarov VN, 1995, EUR J PHYCOL, V30, P261, DOI 10.1080/09670269500651031; Meng Q., 2010, P NATL ACAD SCI USA, V1, P1; MILLAR AJ, 1992, PLANT CELL, V4, P1075, DOI 10.1105/tpc.4.9.1075; Miyagishima SY, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms4807; Moore A, 2014, METHODS MOL BIOL, V1158, P13, DOI 10.1007/978-1-4939-0700-7_2; MORSE D, 1989, P NATL ACAD SCI USA, V86, P172, DOI 10.1073/pnas.86.1.172; MORSE DS, 1990, TRENDS BIOCHEM SCI, V15, P262, DOI 10.1016/0968-0004(90)90050-L; Moulager M, 2007, PLANT PHYSIOL, V144, P1360, DOI 10.1104/pp.107.096149; Moulager M, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1000957; Muller NA, 2016, NAT GENET, V48, P89, DOI 10.1038/ng.3447; Mullineaux CW, 2009, J BACTERIOL, V191, P5333, DOI 10.1128/JB.00719-09; Nikaido SS, 2000, PHOTOCHEM PHOTOBIOL, V71, P758, DOI 10.1562/0031-8655(2000)071<0758:DACVIS>2.0.CO;2; Noordally ZB, 2015, BIOCHEMISTRY-US, V54, P171, DOI 10.1021/bi501089x; O'Neill JS, 2011, NATURE, V469, P554, DOI 10.1038/nature09654; Ohta H, 2005, NAT NEUROSCI, V8, P267, DOI 10.1038/nn1395; Olivares-Yanez C, 2016, GENETICS, V204, P163, DOI 10.1534/genetics.116.191064; PITTENDRIGH CS, 1976, J COMP PHYSIOL, V106, P333, DOI 10.1007/BF01417860; Plautz JD, 1997, J BIOL RHYTHM, V12, P204, DOI 10.1177/074873049701200302; Poliner E, 2018, PLANT CELL REP, V37, P1383, DOI 10.1007/s00299-018-2270-0; Poliner E, 2018, ACS SYNTH BIOL, V7, P962, DOI 10.1021/acssynbio.7b00362; Poliner E, 2018, PLANT BIOTECHNOL J, V16, P298, DOI 10.1111/pbi.12772; Poliner E, 2015, PLANT J, V83, P1097, DOI 10.1111/tpj.12944; Querfurth C, 2011, MOL CELL, V43, P713, DOI 10.1016/j.molcel.2011.06.033; Radakovits R, 2012, NAT COMMUN, V3, DOI 10.1038/ncomms1688; Ragni M, 2004, J PLANKTON RES, V26, P433, DOI 10.1093/plankt/fbh044; Ragni M, 2007, J PLANKTON RES, V29, P141, DOI 10.1093/plankt/fbm002; Rensing L, 2002, CHRONOBIOL INT, V19, P807, DOI 10.1081/CBI-120014569; Roenneberg T, 2005, P NATL ACAD SCI USA, V102, P7742, DOI 10.1073/pnas.0501884102; Roy S, 2014, BMC BIOL, V12, DOI 10.1186/s12915-014-0107-z; Rynearson T.A., 2011, LEARNING READ OCEANS; Schmelling NM, 2017, BMC EVOL BIOL, V17, DOI 10.1186/s12862-017-0999-7; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Schneider K, 2009, GENETICS, V181, P917, DOI 10.1534/genetics.108.097808; Schwartz A.S., 2018, GENOME ANNOUNC, V6; Serrano G, 2009, CURR BIOL, V19, P359, DOI 10.1016/j.cub.2009.01.044; Smith SR, 2016, PLOS GENET, V12, DOI 10.1371/journal.pgen.1006490; Takahashi F, 2007, P NATL ACAD SCI USA, V104, P19625, DOI 10.1073/pnas.0707692104; Tei H, 1997, NATURE, V389, P512, DOI 10.1038/39086; Thiriet-Rupert S, 2016, BMC GENOMICS, V17, DOI 10.1186/s12864-016-2610-9; Thommen Q, 2015, FRONT GENET, V6, DOI 10.3389/fgene.2015.00065; vansOoijen G., 2013, PLOS ONE, V8; Verruto J, 2018, P NATL ACAD SCI USA, V115, pE7015, DOI 10.1073/pnas.1718193115; Vieler A, 2012, PLOS GENET, V8, DOI 10.1371/journal.pgen.1003064; Walton KM, 2009, J PHARMACOL EXP THER, V330, P430, DOI 10.1124/jpet.109.151415; Wang DM, 2014, PLOS GENET, V10, DOI 10.1371/journal.pgen.1004094; Wang QT, 2016, PLANT J, V88, P1071, DOI 10.1111/tpj.13307; Wei L, 2017, PLANT J, V89, P1236, DOI 10.1111/tpj.13411; Welsh DK, 2005, METHOD ENZYMOL, V393, P269, DOI 10.1016/S0076-6879(05)93011-5; Welsh DK, 2005, CURR OPIN BIOTECH, V16, P73, DOI 10.1016/j.copbio.2004.12.006; Welsh DK, 2004, CURR BIOL, V14, P2289, DOI 10.1016/j.cub.2004.11.057; WINFREE AT, 1974, SCIENCE, V183, P970, DOI 10.1126/science.183.4128.970; Woelfle MA, 2004, CURR BIOL, V14, P1481, DOI 10.1016/j.cub.2004.08.023; Xiang QJ, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0092086; Yan OY, 1998, P NATL ACAD SCI USA, V95, P8660, DOI 10.1073/pnas.95.15.8660; Yerushalmi S, 2011, MOL ECOL, V20, P1155, DOI 10.1111/j.1365-294X.2010.04962.x; ZEHRING WA, 1984, CELL, V39, P369, DOI 10.1016/0092-8674(84)90015-1	113	7	7	1	9	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0960-7412	1365-313X		PLANT J	Plant J.	JUL	2019	99	1					112	127		10.1111/tpj.14314			16	Plant Sciences	Plant Sciences	IF4WC	WOS:000473081300009	30883973	Green Accepted, Bronze			2021-04-07	
J	Mignerot, L; Avia, K; Luthringer, R; Lipinska, AP; Peters, AF; Cock, JM; Coelho, SM				Mignerot, Laure; Avia, Komlan; Luthringer, Remy; Lipinska, Agnieszka P.; Peters, Akira F.; Cock, J. Mark; Coelho, Susana M.			A key role for sex chromosomes in the regulation of parthenogenesis in the brown alga Ectocarpus	PLOS GENETICS			English	Article							FLUCTUATING SELECTION; READ ALIGNMENT; APOMIXIS; LOCI; EVOLUTION; REPRODUCTION; GENOME; ESTABLISHMENT; POLYMORPHISM; MAINTENANCE	Although evolutionary transitions from sexual to asexual reproduction are frequent in eukaryotes, the genetic bases of these shifts remain largely elusive. Here, we used classic quantitative trait analysis, combined with genomic and transcriptomic information to dissect the genetic basis of asexual, parthenogenetic reproduction in the brown alga Ectocarpus. We found that parthenogenesis is controlled by the sex locus, together with two additional autosomal loci, highlighting the key role of the sex chromosome as a major regulator of asexual reproduction. We identify several negative effects of parthenogenesis on male fitness, and different fitness effects of parthenogenetic capacity depending on the life cycle generation. Although allele frequencies in natural populations are currently unknown, we discuss the possibility that parthenogenesis may be under both sex-specific selection and generation/ploidally-antagonistic selection, and/or that the action of fluctuating selection on this trait may contribute to the maintenance of polymorphisms in populations. Importantly, our data provide the first empirical illustration, to our knowledge, of a trade-off between the haploid and diploid stages of the life cycle, where distinct parthenogenesis alleles have opposing effects on sexual and asexual reproduction and may help maintain genetic variation. These types of fitness trade-offs have profound evolutionary implications in natural populations and may structure life history evolution in organisms with haploid-diploid life cycles. Author summary Asexual reproduction is widespread among all major clades of eukaryotes. Parthenogenesis represents a specific mode of asexual reproduction, secondarily derived from sexual reproduction, and refers to the development of a multicellular organism from an unfertilised gamete. Parthenogenesis has evolved independently in a wide variety of groups, but the genetic basis and the evolutionary forces driving transitions from sexual to parthenogenetic reproduction remain elusive. Here, we explore genetic, genomic and transcriptomic data from the brown alga Ectocarpus to uncover the genetic architecture of parthenogenesis. The brown algae are a group of complex multicellular organisms that have been evolving independently from animals and plants for more than a billion years, and they have recently emerged as important models to study the evolution of reproductive modes. We show that parthenogenesis is a complex genetic trait under the control of the sex locus, together with two additional autosomal quantitative trait loci, highlighting the critical role for the sex chromosomes in the control of asexual reproduction in this organism. We identify several negative effects of parthenogenesis on male fitness and reveal evidence for trade-offs between sexual and asexual reproduction during the life cycle of Ectocarpus. Our results support the idea that parthenogenesis may be under both sex-specific selection and generation/ploidally-antagonistic selection, but the action of fluctuating selection on this trait may also contribute to the maintenance of polymorphisms in populations.	[Mignerot, Laure; Avia, Komlan; Luthringer, Remy; Lipinska, Agnieszka P.; Cock, J. Mark; Coelho, Susana M.] UPMC Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, CNRS,Algal Genet Grp,UMR 8227,Stn Biol Roscoff, Roscoff, France; [Peters, Akira F.] Bezhin Rosko, Santec, France; [Avia, Komlan] Univ Strasbourg, INRA, SVQV UMR A 1131, Colmar, France	Avia, K; Coelho, SM (corresponding author), UPMC Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, CNRS,Algal Genet Grp,UMR 8227,Stn Biol Roscoff, Roscoff, France.; Avia, K (corresponding author), Univ Strasbourg, INRA, SVQV UMR A 1131, Colmar, France.	komlan.avia@inra.fr; coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020; Avia, Komlan/E-6850-2015	Avia, Komlan/0000-0001-6212-6774; Cock, J. Mark/0000-0002-2650-0383	CNRS, Sorbonne Universite; ERCEuropean Research Council (ERC)European Commission [638240]; ANR (IDEALG project)French National Research Agency (ANR) [10-BTBR-0004]	This work was supported by the CNRS, Sorbonne Universite, the ERC (grant agreement 638240 to SMC) and ANR (IDEALG project 10-BTBR-0004). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Abeel T, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gkr995; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Aitken RJ, 2010, MOL HUM REPROD, V16, P3, DOI 10.1093/molehr/gap059; Anders S, 2015, BIOINFORMATICS, V31, P166, DOI 10.1093/bioinformatics/btu638; Asker S. E, 1992, APOMIXIS PLANTS; Avia K, 2018, GENES-BASEL, V9, DOI 10.3390/genes9060286; Avia K, 2017, SCI REP-UK, V7, DOI 10.1038/srep43241; Barcaccia G, 2013, PLANT REPROD, V26, P159, DOI 10.1007/s00497-013-0222-y; Bell G., 1982, MASTERPIECE NATURE E; Betancourt AJ, 2009, CURR BIOL, V19, P655, DOI 10.1016/j.cub.2009.02.039; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Bonduriansky R, 2009, TRENDS ECOL EVOL, V24, P280, DOI 10.1016/j.tree.2008.12.005; Bothwell JH, 2010, PLANT SIGNAL BEHAV, V5, P1473, DOI 10.4161/psb.5.11.13520; Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Brelsford A, 2017, MOL ECOL RESOUR, V17, P752, DOI 10.1111/1755-0998.12624; Catanach AS, 2006, P NATL ACAD SCI USA, V103, P18650, DOI 10.1073/pnas.0605588103; Catchen J, 2013, MOL ECOL, V22, P3124, DOI 10.1111/mec.12354; CHARLESWORTH B, 1978, J THEOR BIOL, V73, P347, DOI 10.1016/0022-5193(78)90195-9; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P262, DOI 10.1101/pdb.prot067942; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Comeron JM, 2008, HEREDITY, V100, P19, DOI 10.1038/sj.hdy.6801059; Conner JA, 2015, P NATL ACAD SCI USA, V112, P11205, DOI 10.1073/pnas.1505856112; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; Danecek P, 2011, BIOINFORMATICS, V27, P2156, DOI 10.1093/bioinformatics/btr330; EWING EP, 1977, GENETICS, V87, P195; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Grossniklaus U, 2001, PLANT CELL, V13, P1491, DOI 10.1105/tpc.13.7.1491; Han JW, 2014, PLANTA, V240, P1253, DOI 10.1007/s00425-014-2148-5; Harrison XA, 2014, PEERJ, V2, DOI 10.7717/peerj.616; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hemsley PA, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0028799; Hoekstra R F, 1987, Experientia Suppl, V55, P59; Hoshino M, 2019, J PHYCOL, V55, P204, DOI 10.1111/jpy.12812; Hughes R. N., 1989, FUNCTIONAL BIOL CLON; Immler S, 2012, EVOLUTION, V66, P55, DOI 10.1111/j.1558-5646.2011.01399.x; Jackson JBC., 1985, POPULATION BIOL EVOL; Kao LR, 1996, MOL CELL BIOL, V16, P168; Koltunow AM, 2003, ANNU REV PLANT BIOL, V54, P547, DOI 10.1146/annurev.arplant.54.110901.160842; Lampert KP, 2008, SEX DEV, V2, P290, DOI 10.1159/000195678; Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]; Lepais O, 2014, MOL ECOL RESOUR, V14, P1314, DOI 10.1111/1755-0998.12273; Li H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324; Lipinska AP, 2017, GENOME BIOL, V18, DOI 10.1186/s13059-017-1201-7; Lipinska AP, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140535; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; Luthringer R, 2015, PERSPECT PHYCOL, V1, P11; Luthringer R, 2014, DETERMINATION DIFFER; Luthringer R, 2015, MOL BIOL EVOL, V32, P2973, DOI 10.1093/molbev/msv173; Matzk F, 2000, PLANT J, V21, P97, DOI 10.1046/j.1365-313x.2000.00647.x; Montecinos AE, 2017, MOL ECOL, V26, P3497, DOI 10.1111/mec.14098; Montecinos AE, 2017, J PHYCOL, V53, P17, DOI 10.1111/jpy.12452; Neiman M, 2014, J EVOLUTION BIOL, V27, P1346, DOI 10.1111/jeb.12357; Noyes RD, 2000, GENETICS, V155, P379; Ogawa D, 2013, PLANT REPROD, V26, P113, DOI 10.1007/s00497-013-0214-y; Oppliger LV, 2007, J PHYCOL, V43, P1295, DOI 10.1111/j.1529-8817.2007.00408.x; Otto SP, 2015, P NATL ACAD SCI USA, V112, P15952, DOI 10.1073/pnas.1512004112; Otto SP, 2002, NAT REV GENET, V3, P252, DOI 10.1038/nrg761; Paris JR, 2017, METHODS ECOL EVOL, V8, P1360, DOI 10.1111/2041-210X.12775; Rastas P, 2017, BIOINFORMATICS, V33, P3726, DOI 10.1093/bioinformatics/btx494; Reinhold K, 2000, J EVOLUTION BIOL, V13, P1009, DOI 10.1046/j.1420-9101.2000.00229.x; Savidan YH, 2000, ASEXUAL REPROD GENET; Schindelin J, 2012, NAT METHODS, V9, P676, DOI [10.1038/NMETH.2019, 10.1038/nmeth.2019]; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Soriano M, 2013, PLANT REPROD, V26, P181, DOI 10.1007/s00497-013-0226-7; Spillane C, 2004, NAT BIOTECHNOL, V22, P687, DOI 10.1038/nbt976; STALKER HD, 1956, EVOLUTION, V10, P345, DOI 10.1111/j.1558-5646.1956.tb02862.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Oppliger LV, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0102518; Van Dijk PJ, 1999, HEREDITY, V83, P715, DOI 10.1038/sj.hdy.6886200; Visscher PM, 1996, GENET RES, V68, P55, DOI 10.1017/S0016672300033887; Wittmann MJ, 2017, P NATL ACAD SCI USA, V114, pE9932, DOI 10.1073/pnas.1702994114; Xu SZ, 1996, GENETICS, V143, P1417; Zhang JJ, 2014, BIOINFORMATICS, V30, P614, DOI 10.1093/bioinformatics/btt593	76	4	4	1	5	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1553-7404			PLOS GENET	PLoS Genet.	JUN	2019	15	6							e1008211	10.1371/journal.pgen.1008211			25	Genetics & Heredity	Genetics & Heredity	II8TK	WOS:000475464900031	31194744	DOAJ Gold, Green Published			2021-04-07	
J	Liu, T; Wang, XM; Wang, GL; Jia, SG; Liu, GM; Shan, GL; Chi, S; Zhang, J; Yu, YH; Xue, T; Yu, J				Liu, Tao; Wang, Xumin; Wang, Guoliang; Jia, Shangang; Liu, Guiming; Shan, Guangle; Chi, Shan; Zhang, Jing; Yu, Yahui; Xue, Ting; Yu, Jun			Evolution of Complex Thallus Alga: Genome Sequencing of Saccharina japonica	FRONTIERS IN GENETICS			English	Article						Saccharine japonica; genome sequencing; virus genome; phylogenetic analysis; extracellular components; halogen biosynthesis	STRUCTURAL CHARACTERISTICS; SULFATED POLYSACCHARIDES; LAMINARIA-JAPONICA; BROWN-ALGAE; DNA; FUCOIDAN; IDENTIFICATION; ANTIOXIDANT; IODINE	Saccharina, as one of the most important brown algae (Phaeophyceae) with multicellular thallus, has a very remarkable evolutionary history, and globally accounts for most of the economic marine aquaculture production worldwide. Here, we present the 580.5 million base pairs of genome sequence of Saccharina japonica, whose current assembly contains 35,725 protein-coding genes. In a comparative analysis with Ectocarpus siliculosus, the integrated virus sequence suggested the genome evolutionary footprints, which derived from their co-ancestry and experienced genomic arrangements. Furthermore, the gene expansion was found to be an important strategy for functional evolution, especially with regard to extracelluar components, stress-related genes, and vanadium-dependent haloperoxidases, and we proposed a hypothesis that gene duplication events were the main driving force for the evolution history from multicellular filamentous algae to thallus algae. The sequenced Saccharina genome paves the way for further molecular studies and is useful for genome-assisted breeding of S. japonica and other related algae species.	[Liu, Tao; Chi, Shan; Yu, Yahui] Ocean Univ China, Coll Marine Life Sci, Qingdao, Shandong, Peoples R China; [Liu, Tao; Wang, Xumin] Yantai Univ, Coll Life Sci, Yantai, Peoples R China; [Wang, Guoliang; Yu, Jun] Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing Key Lab Genome & Precis Med Technol, Beijing, Peoples R China; [Wang, Guoliang; Shan, Guangle; Yu, Jun] Univ Chinese Acad Sci, Beijing, Peoples R China; [Jia, Shangang] China Agr Univ, Coll Grassland Sci & Technol, Beijing, Peoples R China; [Liu, Guiming] Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing, Peoples R China; [Chi, Shan] Qingdao Haide Blue Tek Biotechnol Co Ltd, Qingdao, Shandong, Peoples R China; [Zhang, Jing] Qilu Univ Technol, Shandong Acad Sci, Coll Biol Engn, Jinan, Shandong, Peoples R China; [Xue, Ting] Fujian Normal Univ, Publ Serv Platform Industrializat Dev Technol Mar, State Ocean Adm, Coll Life Sci, Fuzhou, Fujian, Peoples R China	Liu, T (corresponding author), Ocean Univ China, Coll Marine Life Sci, Qingdao, Shandong, Peoples R China.; Liu, T (corresponding author), Yantai Univ, Coll Life Sci, Yantai, Peoples R China.; Yu, J (corresponding author), Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing Key Lab Genome & Precis Med Technol, Beijing, Peoples R China.; Yu, J (corresponding author), Univ Chinese Acad Sci, Beijing, Peoples R China.; Jia, SG (corresponding author), China Agr Univ, Coll Grassland Sci & Technol, Beijing, Peoples R China.; Xue, T (corresponding author), Fujian Normal Univ, Publ Serv Platform Industrializat Dev Technol Mar, State Ocean Adm, Coll Life Sci, Fuzhou, Fujian, Peoples R China.	liutao@ouc.edu.cn; jsg200830@163.com; xueting@fjnu.edu.cn; junyu@big.ac.cn	dong, wang/T-9093-2019		National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41376143]; Science and Technology Major Project of Fujian Province [2019NZ08003]; Leading Talents Program in Taishan Industry of Shandong Province; seed industry innovation and industrialization project of Fujian Province [2017FJSCZY01]; 13th Five-Year Plan for the Marine Innovation and Economic Development Demonstration Projects [FZHJ1]; China Agriculture Research System-50	This study was funded by the National Natural Science Foundation of China (41376143); Science and Technology Major Project of Fujian Province (2019NZ08003); Leading Talents Program in Taishan Industry of Shandong Province; the seed industry innovation and industrialization project of Fujian Province (2017FJSCZY01); the 13th Five-Year Plan for the Marine Innovation and Economic Development Demonstration Projects (FZHJ1); and China Agriculture Research System-50.	BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Burton JN, 2013, NAT BIOTECHNOL, V31, P1119, DOI 10.1038/nbt.2727; Chi S., 2018, GENOM PROTEOM BIOINF; Chi S, 2018, CURR GENET, V64, P259, DOI 10.1007/s00294-017-0733-4; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; De Smet I, 2009, NAT CELL BIOL, V11, P1166, DOI 10.1038/ncb1009-1166; Demirbas A, 2010, ENERG CONVERS MANAGE, V51, P2738, DOI 10.1016/j.enconman.2010.06.010; Draisma SGA, 2001, J PHYCOL, V37, P586, DOI 10.1046/j.1529-8817.2001.037004586.x; Edel KH, 2017, CURR BIOL, V27, pR667, DOI 10.1016/j.cub.2017.05.020; Erting L, 2004, EUR J PHYCOL, V39, P243, DOI 10.1080/09670260410001712563; Frith MC, 2010, NUCLEIC ACIDS RES, V38, DOI 10.1093/nar/gkq010; Guillemaut P, 1992, PLANT MOL BIOL REP, V10, P60, DOI 10.1007/BF02669265; Huang L, 2010, PHARM BIOL, V48, P422, DOI 10.3109/13880200903150435; Kanehisa M, 2004, NUCLEIC ACIDS RES, V32, pD277, DOI 10.1093/nar/gkh063; Kim KH, 2006, BIOTECHNOL LETT, V28, P439, DOI 10.1007/s10529-005-6177-9; Korbel JO, 2013, NAT BIOTECHNOL, V31, P1099, DOI 10.1038/nbt.2764; Lane CE, 2006, J PHYCOL, V42, P493, DOI 10.1111/j.1529-8817.2006.00204.x; Lane CE, 2008, TRENDS ECOL EVOL, V23, P268, DOI 10.1016/j.tree.2008.02.004; Leblanc C, 2006, BIOCHIMIE, V88, P1773, DOI 10.1016/j.biochi.2006.09.001; Lee AM, 1998, VIROLOGY, V248, P35, DOI 10.1006/viro.1998.9245; Li L, 2003, GENOME RES, V13, P2178, DOI 10.1101/gr.1224503; Liang XY, 2014, ACTA OCEANOL SIN, V33, P27, DOI 10.1007/s13131-014-0438-1; Meints RH, 2008, J VIROL, V82, P1407, DOI 10.1128/JVI.01983-07; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Mizuta H, 2010, BOT MAR, V53, P409, DOI 10.1515/BOT.2010.047; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; Ronquist F, 2003, BIOINFORMATICS, V19, P1572, DOI 10.1093/bioinformatics/btg180; SAENKO GN, 1978, MAR BIOL, V47, P243, DOI 10.1007/BF00541002; Servant N, 2015, GENOME BIOL, V16, DOI 10.1186/s13059-015-0831-x; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tatusov RL, 2003, BMC BIOINFORMATICS, V4, DOI 10.1186/1471-2105-4-41; VALENTIN K, 1990, PLANT MOL BIOL, V15, P575, DOI 10.1007/BF00017832; Vishchuk OS, 2012, CHEM BIODIVERS, V9, P817, DOI 10.1002/cbdv.201100266; Vishchuk OS, 2011, CARBOHYD RES, V346, P2769, DOI 10.1016/j.carres.2011.09.034; Wang J, 2008, INT J BIOL MACROMOL, V42, P127, DOI 10.1016/j.ijbiomac.2007.10.003; Wang J, 2012, J ETHNOPHARMACOL, V139, P807, DOI 10.1016/j.jep.2011.12.022; Xue CH, 2001, J APPL PHYCOL, V13, P67, DOI 10.1023/A:1008103611522; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Yoon HS, 2001, MOL PHYLOGENET EVOL, V21, P231, DOI 10.1006/mpev.2001.1009; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847; Zemke-White WL, 1999, J APPL PHYCOL, V11, P369, DOI 10.1023/A:1008197610793; Zhao X, 2004, J APPL PHYCOL, V16, P111, DOI 10.1023/B:JAPH.0000044822.10744.59; Zhao X, 2012, THROMB RES, V129, P771, DOI 10.1016/j.thromres.2011.07.041	43	3	3	2	11	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND		1664-8021		FRONT GENET	Front. Genet.	MAY 2	2019	10								378	10.3389/fgene.2019.00378			10	Genetics & Heredity	Genetics & Heredity	HW2UD	WOS:000466543100001	31118944	DOAJ Gold, Green Published			2021-04-07	
J	Buaya, AT; Ploch, S; Thines, M				Buaya, Anthony T.; Ploch, Sebastian; Thines, Marco			Rediscovery and phylogenetic placement of Olpidiopsis gillii (de Wildeman) Friedmann, a holocarpic oomycete parasitoid of freshwater diatoms	MYCOSCIENCE			English	Article						Basal oomycetes; Ectrogella; Gyrosigma; Phylogeny; Straminipila	RED ALGAE; SP-NOV; ECTOCARPUS-SILICULOSUS; EURYCHASMA-DICKSONII; MARINE; EVOLUTION; INFECTION; PATHOGENS	The genus Olpidiopsis of the Oomycota includes several species that are aquatic parasites and hyperparasites. Despite their widespread occurrence and potential ecological importance, only a handful of these species has been subjected to phylogenetic investigations, so far. Most species have not been observed and reported for several decades. In the current study, the freshwater diatom parasite Olpidiopsis gillii (de Wild.) Friedmann was rediscovered from the river Main in Germany and investigated for its phylogenetic placement using nuclear small ribosomal subunit (SSU) sequences. The absence of a zoospore diplanetism is a characteristic of the genus Olpidiopsis, which is in contrast to the diplanetism observed in species of Ectrogella. The phylogenetic reconstruction revealed that Olpidiopsis gillii is a basal lineage within the oomycetes, grouping together with the recently- described marine diatom parasite Olpidiopsis drebesii with high support, and loosely associated with Olpidiopsis species parasitising red algae. However, as there are no sequence data available for the type species of both Olpidiopsis and Ectrogella the taxonomic assignment of these simple holocarpic parasites of algae and diatoms remains fraught with uncertainty. (C) 2019 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved.	[Buaya, Anthony T.; Thines, Marco] Goethe Univ, Dept Biol Sci, Inst Ecol Evolut & Divers, Max von Laue Str 13, D-60438 Frankfurt, Germany; [Buaya, Anthony T.; Ploch, Sebastian; Thines, Marco] Senckenberg Biodivers & Climate Res Ctr, Senckenberganlage 25, D-60325 Frankfurt, Germany; [Thines, Marco] Cluster Integrat Fungal Res IPF, Georg Voigt Str 14-16, D-60325 Frankfurt, Germany	Thines, M (corresponding author), Senckenberg Biodivers & Climate Res Ctr, Senckenberganlage 25, D-60325 Frankfurt, Germany.	m.thines@thines-lab.eu			Katholischer Akademischer Auslander-Dienst (KAAD); LOEWE excellence initiative of the government of Hessen in the framework of the Cluster for Integrative Fungal Research (IPF)	AB would like to acknowledge Katholischer Akademischer Auslander-Dienst (KAAD) for a three-year PhD Scholarship. This study was supported by the LOEWE excellence initiative of the government of Hessen in the framework of the Cluster for Integrative Fungal Research (IPF). The funders had no influence on the present manuscript at any stage of the study. We are grateful for the helpful comments of three anonymous referees and the editors.	Anthony TB, 2017, MYCOL PROG, V16, P1041, DOI 10.1007/s11557-017-1345-6; Atami H, 2009, FISH PATHOL, V44, P145, DOI 10.3147/jsfp.44.145; Barrett JT, 1912, ANN BOT-LONDON, V26, P209, DOI 10.1093/oxfordjournals.aob.a089386; Beakes G. W, 2016, HDB PROTISTS, DOI [10.1007/978-3-319-32669-6_26-1, DOI 10.1007/978-3-319-32669-6_26-1]; Beakes GW, 2012, PROTOPLASMA, V249, P3, DOI 10.1007/s00709-011-0269-2; BORTNICK RN, 1985, MYCOLOGIA, V77, P861, DOI 10.2307/3793298; Chukanhom Kanit, 2003, Mycoscience, V44, P123, DOI 10.1007/s10267-003-0090-7; Cornu M., 1872, MONOGRAPHIE SAPROLEG, V15, P1; CZECZUGA B, 1980, MYCOLOGIA, V72, P702, DOI 10.2307/3759763; Czeczuga B, 2002, LIMNOLOGICA, V32, P180, DOI 10.1016/S0075-9511(02)80007-X; de Wildeman E, 1896, ANN SOC BELGE MICRO, V20, P21; Derevnina L, 2016, PHILOS T R SOC B, V371, DOI 10.1098/rstb.2015.0459; Dick MW., 2001, STRAMINIPILOUS FUNGI; DREBES G, 1966, HELGOLAND WISS MEER, V13, P426, DOI 10.1007/BF01611959; DRECHSLER CHARLES, 1940, JOUR WASHINGTON ACAD SCI, V30, P240; Fletcher K, 2015, DIS AQUAT ORGAN, V117, P45, DOI 10.3354/dao02930; Friedmann I, 1952, OSTERR BOT Z, VZ 99, P173; Gill C. H., 1893, J ROYAL MICROSCOPICA, V1, P1; Glockling Sally L., 2000, Fungal Diversity, V4, P1; Hakariya Masateru, 2002, Mycoscience, V43, P119, DOI 10.1007/s102670200018; Hanic LA, 2009, BOTANY, V87, P1096, DOI 10.1139/B09-070; HATAI K, 1980, T MYCOL SOC JPN, V21, P47; Hatai Kishio, 2000, Mycoscience, V41, P565, DOI 10.1007/BF02460922; Huang SC, 2013, CLIMATIC CHANGE, V116, P631, DOI 10.1007/s10584-012-0586-2; Karling JS, 1942, SIMPLE HOLOCARPIC BI; Katoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010; Kitancharoen Nilubol, 1995, Mycoscience, V36, P97, DOI 10.1007/BF02268578; Klochkova TA, 2017, ALGAL RES, V28, P264, DOI 10.1016/j.algal.2017.09.019; Klochkova TA, 2016, J APPL PHYCOL, V28, P73, DOI 10.1007/s10811-015-0595-4; Lange-Bertalot H, 1979, NOVA HEDWIGIA, V1, P635, DOI [10.1127/nova.hedwigia/30/1979/635, DOI 10.1127/NOVA.HEDWIGIA/30/1979/635]; Lara E, 2011, FUNGAL DIVERS, V49, P93, DOI 10.1007/s13225-011-0098-9; Li W, 2010, ACTA OCEANOL SIN, V29, P74, DOI 10.1007/s13131-010-0065-4; Magnus P., 1905, HEDWIGIA, V44, P347; MARTIN RW, 1986, MYCOLOGIA, V78, P359, DOI 10.2307/3793039; Massana R, 2004, APPL ENVIRON MICROB, V70, P3528, DOI 10.1128/AEM.70.6.3528-3534.2004; Massana R, 2006, ENVIRON MICROBIOL, V8, P1515, DOI 10.1111/j.1462-2920.2006.01042.x; Molloy DP, 2014, FUNGAL BIOL-UK, V118, P544, DOI 10.1016/j.funbio.2014.01.007; Nakamura Kazuyo, 1994, Mycoscience, V35, P383, DOI 10.1007/BF02268509; Raffaele S, 2012, NAT REV MICROBIOL, V10, P417, DOI 10.1038/nrmicro2790; Raven JA, 2004, NEW PHYTOL, V162, P45, DOI 10.1111/j.1469-8137.2004.01022.x; Schenk A., 1859, VERHANDL PHYS MED GE, V9, P12; Scherffel A., 1925, Archiv fuer Protistenkunde Jena, V52, P1; SCHNEPF E, 1977, HELGOLAND WISS MEER, V29, P291, DOI 10.1007/BF01614265; Scholz B, 2016, EUR J PHYCOL, V51, P253, DOI 10.1080/09670262.2015.1134814; Scholz B, 2016, FUNGAL ECOL, V19, P59, DOI 10.1016/j.funeco.2015.09.002; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Sekimoto S, 2009, PHYCOLOGIA, V48, P460, DOI 10.2216/08-11.1; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES; Strittmatter M, 2009, OOMYCETE GENETICS GE, P25; Strittmatter M, 2016, PLANT CELL ENVIRON, V39, P259, DOI 10.1111/pce.12533; TAKAHASHI M, 1977, T MYCOL SOC JPN, V18, P279; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Thines M, 2015, MYCOL PROG, V14, DOI 10.1007/s11557-015-1099-y; Thines M, 2014, EUR J PLANT PATHOL, V138, P431, DOI 10.1007/s10658-013-0366-5; Van West Pieter, 2006, Mycologist, V20, P99, DOI 10.1016/j.mycol.2006.06.004; VISHNIAC HS, 1958, MYCOLOGIA, V50, P66, DOI 10.2307/3756037; Voigt K., 2013, MYCOTA, P243, DOI 10.1007/978-3-642-36821-9_9; Wang YP, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0077810; Zopf F. W., 1884, NOVA ACTA ACAD CAESA, V47, P143	59	4	8	0	8	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1340-3540	1618-2545		MYCOSCIENCE	Mycoscience	MAY	2019	60	3					141	146		10.1016/j.myc.2019.01.002			6	Mycology	Mycology	HZ3JA	WOS:000468743300002					2021-04-07	
J	Inoue, A; Ojima, T				Inoue, Akira; Ojima, Takao			Functional identification of alginate lyase from the brown alga Saccharina japonica	SCIENTIFIC REPORTS			English	Article							4-DEOXY-L-ERYTHRO-5-HEXOSEULOSE URONIC-ACID; VINELANDII MANNURONAN C-5-EPIMERASE; ZOBELLIA-GALACTANIVORANS; PSEUDOMONAS-FLUORESCENS; LIGHT-SCATTERING; PECTATE LYASE; EXPRESSION; METABOLISM; ECTOCARPUS; INSIGHTS	Despite the progress in massive gene analysis of brown algal species, no alginate-degrading enzyme from brown alga has been identified, impeding the understanding of alginate metabolism in brown alga. In the current study, we identified and characterized alginate lyase from Saccharina japonica using a protein-based approach. First, cDNA library was prepared from the S. japonica sporophyte. Expression screening was then performed; the encoding gene was identified and cloned; and the recombinant enzyme was purified and characterized. Alginate lyase production in algal tissues was evaluated by western blotting. The identified alginate lyase, SjAly (359 amino acids, with a predicted N-terminal secretion signal of 27 residues), is encoded by an open reading frame comprising seven exons. Recombinant SjAly exhibited endolytic alginate lyase activity, specifically toward stretches of consecutive ss-D-mannuronic acid units. The optimum temperature, pH, and NaCl concentration were 30 degrees C, pH 8.0, and 100 mM, respectively. SjAly exhibited pronounced activity below 20 degrees C, the S. japonica growth temperature. SjAly was highly expressed in the blade but not the stipe and rhizoid. The data indicate that S. japonica possesses at least one active alginate lyase. This is the first report of a functional alginate lyase from brown alga, the major natural alginate producer.	[Inoue, Akira; Ojima, Takao] Hokkaido Univ, Grad Sch Fisheries Sci, Lab Marine Biotechnol & Microbiol, 3-1-1 Minatocho, Hakodate, Hokkaido, Japan	Inoue, A (corresponding author), Hokkaido Univ, Grad Sch Fisheries Sci, Lab Marine Biotechnol & Microbiol, 3-1-1 Minatocho, Hakodate, Hokkaido, Japan.	inouea21@fish.hokudai.ac.jp			JSPS KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [16H04977]	This work was supported by JSPS KAKENHI (grant number 16H04977). We thank the Instrumental Analysis Division, Global Facility Center, Creative Research Institution, Hokkaido University for the ESI-MS with an Exactive Mass Spectrometer and providing insight and expertise that greatly assisted the research.	Albrecht MT, 2005, J BACTERIOL, V187, P3869, DOI 10.1128/JB.187.11.3869-3872.2005; Andresen I.-L., 1977, CELLULOSE CHEM TECHN, P361, DOI [10.1021/bk- 1977-0048.ch024, DOI 10.1021/BK-1977-0048.CH024]; Bakkevig K, 2005, J BACTERIOL, V187, P8375, DOI 10.1128/JB.187.24.8375-8384.2005; CALLOW ME, 1978, J CELL SCI, V32, P337; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Draget KI, 2011, FOOD HYDROCOLLOID, V25, P251, DOI 10.1016/j.foodhyd.2009.10.007; ERTESVAG H, 1994, J BACTERIOL, V176, P2846; Ertesvag H, 2015, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.00523; Fischl R, 2016, GLYCOBIOLOGY, V26, P973, DOI 10.1093/glycob/cww040; FRANKLIN MJ, 1994, J BACTERIOL, V176, P1821, DOI 10.1128/JB.176.7.1821-1830.1994; GACESA P, 1990, APPL ENVIRON MICROB, V56, P2265, DOI 10.1128/AEM.56.7.2265-2267.1990; GORIN PAJ, 1966, CAN J CHEMISTRY, V44, P993, DOI 10.1139/v66-147; GOVAN JRW, 1981, J GEN MICROBIOL, V125, P217; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; GRASDALEN H, 1983, CARBOHYD RES, V118, P255, DOI 10.1016/0008-6215(83)88053-7; Haas BJ, 2013, NAT PROTOC, V8, P1494, DOI 10.1038/nprot.2013.084; HABERMANN E, 1967, H-S Z PHYSIOL CHEM, V348, P37, DOI 10.1515/bchm2.1967.348.1.37; Hassan S, 2013, J BACTERIOL, V195, P2197, DOI 10.1128/JB.02118-12; HAUG A, 1970, ACTA CHEM SCAND, V24, P843, DOI 10.3891/acta.chem.scand.24-0843; HAUG A, 1967, NATURE, V215, P757, DOI 10.1038/215757a0; INDERGAARD M, 1987, HYDROBIOLOGIA, V151, P541, DOI 10.1007/BF00046180; Inoue A, 2018, METHOD ENZYMOL, V605, P499, DOI 10.1016/bs.mie.2018.01.030; Inoue A, 2016, ALGAL RES, V19, P355, DOI 10.1016/j.algal.2016.03.008; Inoue A, 2016, ALGAL RES, V16, P282, DOI 10.1016/j.algal.2016.03.030; Inoue A, 2015, MAR DRUGS, V13, P493, DOI 10.3390/md13010493; Inoue A, 2014, MAR DRUGS, V12, P4693, DOI 10.3390/md12084693; Inoue A, 2011, MAR BIOTECHNOL, V13, P256, DOI 10.1007/s10126-010-9290-2; Jenkins J, 2004, J BIOL CHEM, V279, P9139, DOI 10.1074/jbc.M311390200; Jornvall H, 2002, BIOCHEMISTRY-US, V34, P6003; Kelley LA, 2015, NAT PROTOC, V10, P845, DOI 10.1038/nprot.2015.053; Lee EJ, 2018, MAR BIOTECHNOL, V20, P410, DOI 10.1007/s10126-018-9805-9; LINKER A, 1966, J BIOL CHEM, V241, P3845; LINKER A, 1964, NATURE, V204, P187, DOI 10.1038/204187a0; Lyons E, 2008, PLANT J, V53, P661, DOI 10.1111/j.1365-313X.2007.03326.x; MCKEE JWA, 1992, J APPL PHYCOL, V4, P357, DOI 10.1007/BF02185794; Michel G, 2004, J BIOL CHEM, V279, P32882, DOI 10.1074/jbc.M403421200; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Mochizuki S, 2015, J BIOL CHEM, V290, P30962, DOI 10.1074/jbc.M115.686725; Moradali MF, 2018, SPRING SER BIOMAT S, V11, P1, DOI 10.1007/978-981-10-6910-9_1; Moummou H, 2012, BMC PLANT BIOL, V12, DOI 10.1186/1471-2229-12-219; Nagasato C, 2010, PLANTA, V232, P287, DOI 10.1007/s00425-010-1188-8; Nagasato C, 2009, J PHYCOL, V45, P404, DOI 10.1111/j.1529-8817.2009.00655.x; Nishiyama R, 2017, MAR DRUGS, V15, DOI 10.3390/md15020037; Njau RK, 2001, CHEM-BIOL INTERACT, V130, P785, DOI 10.1016/S0009-2797(00)00234-9; Penning TM, 2015, CHEM-BIOL INTERACT, V234, P236, DOI 10.1016/j.cbi.2014.09.024; Percival E., 2007, BR PHYCOL J, V14, P103; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; PREISS J, 1962, J BIOL CHEM, V237, P309; PREISS J, 1962, J BIOL CHEM, V237, P317; SCHILLER NL, 1993, J BACTERIOL, V175, P4780, DOI 10.1128/JB.175.15.4780-4789.1993; Schoenwaelder MEA, 2000, PLANT BIOLOGY, V2, P24, DOI 10.1055/s-2000-9178; Seyedarabi A, 2010, BIOCHEMISTRY-US, V49, P539, DOI 10.1021/bi901503g; SKJAKBRAEK G, 1989, CARBOHYD RES, V185, P119, DOI 10.1016/0008-6215(89)84027-3; SMIDSROD O, 1975, FARADAY DISCUSS, V57, P263; SMIDSROD O, 1969, MACROMOLECULES, V2, P42, DOI 10.1021/ma60007a008; STOKKE BT, 1993, CARBOHYD POLYM, V22, P57, DOI 10.1016/0144-8617(93)90166-2; Storz H, 2009, CARBOHYD RES, V344, P985, DOI 10.1016/j.carres.2009.02.016; Svanem BIG, 1999, J BACTERIOL, V181, P68, DOI 10.1128/JB.181.1.68-77.1999; Svanem BIG, 2001, J BIOL CHEM, V276, P31542, DOI 10.1074/jbc.M102562200; Takase R, 2014, J BIOL CHEM, V289, P33198, DOI 10.1074/jbc.M114.585661; Takase R, 2010, BBA-PROTEINS PROTEOM, V1804, P1925, DOI 10.1016/j.bbapap.2010.05.010; Thomas F, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.01808; Thomas F, 2012, ENVIRON MICROBIOL, V14, P2379, DOI 10.1111/j.1462-2920.2012.02751.x; Thomas F, 2013, J BIOL CHEM, V288, P23021, DOI 10.1074/jbc.M113.467217; VENEGAS M, 1993, BOT MAR, V36, P47, DOI 10.1515/botm.1993.36.1.47; VLASAK R, 1983, EUR J BIOCHEM, V135, P123, DOI 10.1111/j.1432-1033.1983.tb07626.x; Vold IMN, 2006, BIOMACROMOLECULES, V7, P2136, DOI 10.1021/bm060099n; Wang Y., 2016, SCI REP, V6, P495; Windhues T, 2003, CARBOHYD POLYM, V52, P47, DOI 10.1016/S0144-8617(02)00265-5; Winn MD, 2011, ACTA CRYSTALLOGR D, V67, P235, DOI 10.1107/S0907444910045749; Wong TY, 2000, ANNU REV MICROBIOL, V54, P289, DOI 10.1146/annurev.micro.54.1.289; Xu F, 2017, J BIOL CHEM, V292, P4457, DOI 10.1074/jbc.M116.766030; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986	74	10	10	2	9	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	2045-2322			SCI REP-UK	Sci Rep	MAR 20	2019	9								4937	10.1038/s41598-019-41351-6			11	Multidisciplinary Sciences	Science & Technology - Other Topics	HP5ZR	WOS:000461762600054	30894645	DOAJ Gold, Green Published			2021-04-07	
J	Nishitsuji, K; Arimoto, A; Higa, Y; Mekaru, M; Kawamitsu, M; Satoh, N; Shoguchi, E				Nishitsuji, Koki; Arimoto, Asuka; Higa, Yoshimi; Mekaru, Munekazu; Kawamitsu, Mayumi; Satoh, Noriyuki; Shoguchi, Eiichi			Draft genome of the brown alga, Nemacystus decipiens, Onna-1 strain: Fusion of genes involved in the sulfated fucan biosynthesis pathway	SCIENTIFIC REPORTS			English	Article							ANNOTATION; SEQUENCE; POLYSACCHARIDES; EVOLUTION; MODEL; TOOL	The brown alga, Nemacystus decipiens ("ito-mozuku" in Japanese), is one of the major edible seaweeds, cultivated principally in Okinawa, Japan. N. decipiens is also a significant source of fucoidan, which has various physiological activities. To facilitate brown algal studies, we decoded the similar to 154 Mbp draft genome of N. decipiens Onna-1 strain. The genome is estimated to contain 15,156 protein-coding genes, similar to 78% of which are substantiated by corresponding mRNAs. Mitochondrial genes analysis showed a close relationship between N. decipiens and Cladosiphon okamuranus. Comparisons with the C. okamuranus and Ectocarpus siliculosus genomes identified a set of N. decipiens-specific genes. Gene ontology annotation showed more than half of these are classified as molecular function, enzymatic activity, and/or biological process. Extracellular matrix analysis revealed domains shared among three brown algae. Characterization of genes that encode enzymes involved in the biosynthetic pathway for sulfated fucan showed two sets of genes fused in the genome. One is a fusion of L-fucokinase and GDPfucose pyrophosphorylase genes, a feature shared with C. okamuranus. Another fusion is between an ST-domain-containing gene and an alpha/beta hydrolase gene. Although the function of fused genes should be examined in future, these results suggest that N. decipiens is another promising source of fucoidan.	[Nishitsuji, Koki; Arimoto, Asuka; Satoh, Noriyuki; Shoguchi, Eiichi] Okinawa Inst Sci & Technol Grad Univ, Marine Genom Unit, Onna, Okinawa 9040495, Japan; [Higa, Yoshimi; Mekaru, Munekazu] Onna Fisheries Cooperat, Onna, Okinawa 9040414, Japan; [Kawamitsu, Mayumi] Okinawa Inst Sci & Technol Grad Univ, DNA Sequencing Sect, Onna, Okinawa 9040495, Japan	Nishitsuji, K (corresponding author), Okinawa Inst Sci & Technol Grad Univ, Marine Genom Unit, Onna, Okinawa 9040495, Japan.	koki.nishitsuji@oist.jp	Satoh, Nori/C-4123-2009	Satoh, Nori/0000-0002-4480-3572	OISTOkinawa Institute of Science & Technology Graduate University	We thank Ms. Haruhi Narisoko for culturing Nemacystus decipiens and Mr. Kenji Iwai for the photo of Cladosiphon okamuranus. This research was supported by OIST funding to the Marine Genomics Unit (N.S.).	BABA M, 1988, ANTIMICROB AGENTS CH, V32, P1742, DOI 10.1128/AAC.32.11.1742; Bailey TL, 2009, NUCLEIC ACIDS RES, V37, pW202, DOI 10.1093/nar/gkp335; Bentley DR, 2006, CURR OPIN GENET DEV, V16, P545, DOI 10.1016/j.gde.2006.10.009; Boetzer M, 2011, BIOINFORMATICS, V27, P578, DOI 10.1093/bioinformatics/btq683; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Capella-Gutierrez S, 2009, BIOINFORMATICS, V25, P1972, DOI 10.1093/bioinformatics/btp348; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Daley WP, 2008, J CELL SCI, V121, P255, DOI 10.1242/jcs.006064; Dierckxsens N, 2017, NUCLEIC ACIDS RES, V45, DOI 10.1093/nar/gkw955; Eddy SR, 1998, BIOINFORMATICS, V14, P755, DOI 10.1093/bioinformatics/14.9.755; Emms DM, 2015, GENOME BIOL, V16, DOI 10.1186/s13059-015-0721-2; Finn RD, 2006, NUCLEIC ACIDS RES, V34, pD247, DOI 10.1093/nar/gkj149; Gotz S, 2011, BIOINFORMATICS, V27, P919, DOI 10.1093/bioinformatics/btr059; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Haas BJ, 2003, NUCLEIC ACIDS RES, V31, P5654, DOI 10.1093/nar/gkg770; Hirakawa H, 2014, DNA RES, V21, P169, DOI 10.1093/dnares/dst049; Jarvelainen H, 2009, PHARMACOL REV, V61, P198, DOI 10.1124/pr.109.001289; Jones P, 2014, BIOINFORMATICS, V30, P1236, DOI 10.1093/bioinformatics/btu031; Jurka J, 2005, CYTOGENET GENOME RES, V110, P462, DOI 10.1159/000084979; Kajitani R, 2014, GENOME RES, V24, P1384, DOI 10.1101/gr.170720.113; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Krogh A, 2001, J MOL BIOL, V305, P567, DOI 10.1006/jmbi.2000.4315; Lagesen K, 2007, NUCLEIC ACIDS RES, V35, P3100, DOI 10.1093/nar/gkm160; Lex A, 2014, IEEE T VIS COMPUT GR, V20, P1983, DOI 10.1109/TVCG.2014.2346248; Li RQ, 2010, NATURE, V463, P311, DOI 10.1038/nature08696; LIN TY, 1966, J BIOL CHEM, V241, P5284; Marcais G, 2011, BIOINFORMATICS, V27, P764, DOI 10.1093/bioinformatics/btr011; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Migita S, 1972, B FACUL FISHER, V34, P51; Nishitsuji K, 2016, DNA RES, V23, P561, DOI 10.1093/dnares/dsw039; Nishiyama T, 2018, CELL, V174, P448, DOI 10.1016/j.cell.2018.06.033; NISIZAWA K, 1987, HYDROBIOLOGIA, V151, P5, DOI 10.1007/BF00046102; Parra G, 2007, BIOINFORMATICS, V23, P1061, DOI 10.1093/bioinformatics/btm071; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; Porse H, 2017, J APPL PHYCOL, V29, P2187, DOI 10.1007/s10811-017-1144-0; Price AL, 2005, BIOINFORMATICS, V21, pI351, DOI 10.1093/bioinformatics/bti1018; SAXENA IM, 1995, J BACTERIOL, V177, P1419, DOI 10.1128/jb.177.6.1419-1424.1995; Schulz MH, 2012, BIOINFORMATICS, V28, P1086, DOI 10.1093/bioinformatics/bts094; Sigrist CJA, 2013, NUCLEIC ACIDS RES, V41, pE344, DOI 10.1093/nar/gks1067; Silberfeld T, 2014, CRYPTOGAMIE ALGOL, V35, P117, DOI 10.7872/crya.v35.iss2.2014.117; Skinner ME, 2009, GENOME RES, V19, P1630, DOI 10.1101/gr.094607.109; Stamatakis A, 2014, BIOINFORMATICS, V30, P1312, DOI 10.1093/bioinformatics/btu033; Stanke M, 2008, BIOINFORMATICS, V24, P637, DOI 10.1093/bioinformatics/btn013; Takeuchi T, 2012, DNA RES, V19, P117, DOI 10.1093/dnares/dss005; Tako M, 1999, BIOSCI BIOTECH BIOCH, V63, P1813, DOI 10.1271/bbb.63.1813; Terauchi M, 2017, MAR GENOM, V32, P49, DOI 10.1016/j.margen.2016.12.002; Tillich M, 2017, NUCLEIC ACIDS RES, V45, pW6, DOI 10.1093/nar/gkx391; Van Den Hoek C, 1995, ALGAE INTRO PHYCOLOG; Vurture GW, 2017, BIOINFORMATICS, V33, P2202, DOI 10.1093/bioinformatics/btx153; Wu YW, 2014, MICROBIOME, V2, DOI 10.1186/2049-2618-2-26; Yamada N, 2006, SCI SEAWEED FUCOIDAN; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; YOSHIDA T, 2015, JAPANESE J PHYCOLOGY, V63, P129; Zerbino DR, 2008, GENOME RES, V18, P821, DOI 10.1101/gr.074492.107	56	8	8	1	9	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	2045-2322			SCI REP-UK	Sci Rep	MAR 14	2019	9								4607	10.1038/s41598-019-40955-2			11	Multidisciplinary Sciences	Science & Technology - Other Topics	HO7WB	WOS:000461159600084	30872679	DOAJ Gold, Green Published			2021-04-07	
J	Teng, LH; Fan, X; Nelson, DR; Han, WT; Zhang, XW; Xu, D; Renault, H; Markov, GV; Ye, NH				Teng, Linhong; Fan, Xiao; Nelson, David R.; Han, Wentao; Zhang, Xiaowen; Xu, Dong; Renault, Hugues; Markov, Gabriel V.; Ye, Naihao			Diversity and evolution of cytochromes P450 in stramenopiles	PLANTA			English	Article						Cytochrome P450; Comparative genomics; Molecular evolution; Stramenopiles	BROWN ALGA; THALASSIOSIRA-PSEUDONANA; PHYLOGENETIC ANALYSIS; COMPARATIVE GENOMICS; GENE DUPLICATION; CYP51; MONOOXYGENASES; BIOSYNTHESIS; FAMILY; MEMBER	Main conclusionComparative genomic analysis of cytochromes P450 revealed high diversification and dynamic changes in stramenopiles, associated with transcriptional responsiveness to various environmental stimuli.Comparative genomic and molecular evolution approaches were used to characterize cytochromes P450 (P450) diversity in stramenopiles. Phylogenetic analysis pointed to a high diversity of P450 in stramenopiles and identified three major clans. The CYP51 and CYP97 clans were present in brown algae, diatoms and Nannochloropsis gaditana, whereas the CYP5014 clan mainly includes oomycetes. Gene gain and loss patterns revealed that six CYP familiesCYP51, CYP97, CYP5160, CYP5021, CYP5022, and CYP5165predated the split of brown algae and diatoms. After they diverged, diatoms gained more CYP families, especially in the cold-adapted species Fragilariopsis cylindrus, in which eight new CYP families were found. Selection analysis revealed that the expanded CYP51 family in the brown alga Cladosiphon okamuranus exhibited a more relaxed selection constraint compared with those of other brown algae and diatoms. Our RNA-seq data further evidenced that most of P450s in Saccharina japonica are highly expressed in large sporophytes, which could potentially promote the large kelp formation in this developmental stage. A survey of Ectocarpus siliculosus and diatom transcriptomes showed that many P450s are responsive to stress, nutrient limitation or light quality, suggesting pivotal roles in detoxification or metabolic processes under adverse environmental conditions. The information provided in this study will be helpful in designing functional experiments and interpreting P450 roles in this particular lineage.	[Teng, Linhong; Fan, Xiao; Han, Wentao; Zhang, Xiaowen; Xu, Dong; Ye, Naihao] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; [Teng, Linhong; Ye, Naihao] Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China; [Nelson, David R.] Univ Tennessee, Hlth Sci Ctr, Dept Microbiol Immunol & Biochem, 858 Madison Ave Suite G01, Memphis, TN 38163 USA; [Renault, Hugues] Univ Strasbourg, CNRS, Inst Plant Mol Biol, F-67084 Strasbourg, France; [Markov, Gabriel V.] Sorbonne Univ, CNRS, SBR, Integrat Biol Marine Models LBI2M, F-29680 Roscoff, France	Ye, NH (corresponding author), Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China.; Ye, NH (corresponding author), Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China.	yenh@ysfri.ac.cn		Renault, Hugues/0000-0002-0871-9912; Markov, Gabriel V./0000-0002-8566-7482	Special Scientific Research Funds for Central Non-Profit Institutes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences [20603022016010, 20603022016001]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41676145]; Shandong key Research and Development Plan [2018GHY115010]; China Agriculture Research System [CARS-50]; Taishan Scholars Funding; AoShan Talents Program [2015ASTPES03]; Science Fund for Distinguished Young Scholars of Shandong Province [JQ201509]; Qingdao Municipal Science and Technology plan project [17-1-1-96-jch]; French Government via the National Research Agency investment expenditure program IDEALG [ANR-10-BTBR-04]; Region BretagneRegion Bretagne [SAD2016-METALG (9673)]; initiative of excellence IDEX-unistra from the French national program "Investment for the future" [ANR-10-IDEX-0002-28102]	This work was supported by Special Scientific Research Funds for Central Non-Profit Institutes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (20603022016010, 20603022016001); National Natural Science Foundation of China (41676145); Shandong key Research and Development Plan (2018GHY115010); China Agriculture Research System (CARS-50); Taishan Scholars Funding; AoShan Talents Program (No. 2015ASTPES03); the Science Fund for Distinguished Young Scholars of Shandong Province (JQ201509); Qingdao Municipal Science and Technology plan project (17-1-1-96-jch). G.V.M. benefited from the support of the French Government via the National Research Agency investment expenditure program IDEALG (ANR-10-BTBR-04) and from Region Bretagne via the grant "SAD2016-METALG (9673)". This work received support from the initiative of excellence IDEX-unistra (ANR-10-IDEX-0002-28102) from the French national program "Investment for the future" to H.R.	Aleoshin VV, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.01194; Almeida D, 2016, GENOME BIOL EVOL, V8, P1115, DOI 10.1093/gbe/evw041; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Andreou A, 2009, PROG LIPID RES, V48, P148, DOI 10.1016/j.plipres.2009.02.002; Ashworth J, 2016, MAR GENOM, V26, P21, DOI 10.1016/j.margen.2015.10.011; Barofsky A, 2007, ORG LETT, V9, P1017, DOI 10.1021/ol063051v; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; Boonprab K, 2003, PHYTOCHEMISTRY, V63, P669, DOI 10.1016/S0031-9422(03)00026-8; Boonprab K, 2003, Z NATURFORSCH C, V58, P207; Brembu T, 2011, ENVIRON SCI TECHNOL, V45, P7640, DOI 10.1021/es2002259; Carvalho RN, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0026985; Chou KC, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0009931; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cock JM, 2012, ECTOCARPUS GENOME BR; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Crooks GE, 2004, GENOME RES, V14, P1188, DOI 10.1101/gr.849004; Csuros M, 2010, BIOINFORMATICS, V26, P1910, DOI 10.1093/bioinformatics/btq315; Csuros M, 2011, PLOS COMPUT BIOL, V7, DOI 10.1371/journal.pcbi.1002150; d'Ippolito G, 2006, PHYTOCHEMISTRY, V67, P314, DOI 10.1016/j.phytochem.2005.11.012; Debeljak N, 2000, ARCH BIOCHEM BIOPHYS, V379, P37, DOI 10.1006/abbi.2000.1859; Deng JX, 2007, BMC EVOL BIOL, V7, DOI 10.1186/1471-2148-7-30; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dorrell RG, 2017, ELIFE, V6, DOI 10.7554/eLife.23717; Eddy SR, 2011, PLOS COMPUT BIOL, V7, DOI 10.1371/journal.pcbi.1002195; FARRIS JS, 1977, SYST ZOOL, V26, P77, DOI 10.2307/2412867; Finn RD, 2016, NUCLEIC ACIDS RES, V44, pD279, DOI 10.1093/nar/gkv1344; Frommolt R, 2008, MOL BIOL EVOL, V25, P2653, DOI 10.1093/molbev/msn206; Geisler K, 2013, P NATL ACAD SCI USA, V110, pE3360, DOI 10.1073/pnas.1309157110; Goldstone HMH, 2006, J MOL EVOL, V62, P708, DOI 10.1007/s00239-005-0134-z; Grigoriev IV, 2014, NUCLEIC ACIDS RES, V42, P26; Gruber A, 2015, PLANT J, V81, P519, DOI 10.1111/tpj.12734; Guengerich FP, 2013, J BIOL CHEM, V288, P17065, DOI 10.1074/jbc.R113.462275; Hu B, 2015, BIOINFORMATICS, V31, P1296, DOI 10.1093/bioinformatics/btu817; Keeling PJ, 2014, PLOS BIOL, V12, DOI 10.1371/journal.pbio.1001889; Keeling PJ, 2013, ANNU REV PLANT BIOL, V64, P583, DOI 10.1146/annurev-arplant-050312-120144; Kelly DE, 2009, FUNGAL GENET BIOL, V46, pS53, DOI 10.1016/j.fgb.2008.08.010; Kersey PJ, 2018, NUCLEIC ACIDS RES, V46, pD802, DOI 10.1093/nar/gkx1011; Kim HB, 2005, PLANT PHYSIOL, V138, P2033, DOI 10.1104/pp.105.061598; Kousaka K, 2003, J NAT PROD, V66, P1318, DOI 10.1021/np030049t; Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]; Lepesheva GI, 2007, BBA-GEN SUBJECTS, V1770, P467, DOI 10.1016/j.bbagen.2006.07.018; Li Z, 2016, PLANT CELL, V28, P326, DOI 10.1105/tpc.15.00877; Liu ZH, 2016, NAT COMMUN, V7, DOI 10.1038/ncomms13026; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; Mizutani M, 2010, ANNU REV PLANT BIOL, V61, P291, DOI 10.1146/annurev-arplant-042809-112305; Mock T, 2008, P NATL ACAD SCI USA, V105, P1579, DOI 10.1073/pnas.0707946105; Mock T, 2017, NATURE, V541, P536, DOI 10.1038/nature20803; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; Nelson D R, 1998, Methods Mol Biol, V107, P15; Nelson DR, 2018, BBA-PROTEINS PROTEOM, V1866, P141, DOI 10.1016/j.bbapap.2017.05.003; Nelson DR, 2013, PHILOS T R SOC B, V368, DOI 10.1098/rstb.2012.0474; Nelson David R., 2009, Human Genomics, V4, P59; Nelson DR, 2004, PLANT PHYSIOL, V135, P756, DOI 10.1104/pp.104.039826; Nishitsuji K, 2016, DNA RES, V23, P561, DOI 10.1093/dnares/dsw039; Nymark M, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0007743; Omura T, 2017, J BIOCHEM, V161, P399, DOI 10.1093/jb/mvx011; Parvez M, 2016, SCI REP-UK, V6, DOI 10.1038/srep33099; Pflugmacher S, 1998, PLANT PHYSIOL, V117, P123, DOI 10.1104/pp.117.1.123; PHILLIPS IR, 1998, CYTOCHROME P450 PROT; PROTEAU PJ, 1992, TETRAHEDRON LETT, V33, P4393, DOI 10.1016/S0040-4039(00)60092-8; Qi X, 2006, P NATL ACAD SCI USA, V103, P18848, DOI 10.1073/pnas.0607849103; Renault H, 2017, MOL BIOL EVOL, V34, P2041, DOI 10.1093/molbev/msx160; Renault H, 2014, CURR OPIN PLANT BIOL, V19, P27, DOI 10.1016/j.pbi.2014.03.004; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Ritter A, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0173315; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Roy SW, 2007, MOL BIOL EVOL, V24, P1447, DOI 10.1093/molbev/msm048; Sapriel G, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0007458; Sello MM, 2015, SCI REP-UK, V5, DOI 10.1038/srep11572; Sevcikova T, 2015, SCI REP-UK, V5, DOI 10.1038/srep10134; Sievers F, 2011, MOL SYST BIOL, V7, DOI 10.1038/msb.2011.75; Suyama M, 2006, NUCLEIC ACIDS RES, V34, pW609, DOI 10.1093/nar/gkl315; Syed K, 2013, MYCOLOGIA, V105, P1445, DOI 10.3852/13-002; Teng LH, 2017, FRONT PLANT SCI, V8, DOI 10.3389/fpls.2017.02018; Teng LH, 2017, FRONT PLANT SCI, V8, DOI 10.3389/fpls.2017.01429; Tian L, 2004, P NATL ACAD SCI USA, V101, P402, DOI 10.1073/pnas.2237237100; Toporkova YY, 1862, BIOCH BIOPHYS ACTA M, V2, P167; Valle KC, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0114211; Voorrips RE, 2002, J HERED, V93, P77, DOI 10.1093/jhered/93.1.77; Yang S, 2003, J PHYCOL, V39, P555, DOI 10.1046/j.1529-8817.2003.02190.x; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Yu LY, 2015, SCI REP-UK, V5, DOI 10.1038/srep08952; Zhao J, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0045092	84	2	2	2	22	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0032-0935	1432-2048		PLANTA	Planta	MAR	2019	249	3					647	661		10.1007/s00425-018-3028-1			15	Plant Sciences	Plant Sciences	HM5BG	WOS:000459488700003	30341489				2021-04-07	
J	Guillot, L; Delage, L; Viari, A; Vandenbrouck, Y; Com, E; Ritter, A; Lavigne, R; Marie, D; Peterlongo, P; Potin, P; Pineau, C				Guillot, Laetitia; Delage, Ludovic; Viari, Alain; Vandenbrouck, Yves; Com, Emmanuelle; Ritter, Andres; Lavigne, Regis; Marie, Dominique; Peterlongo, Pierre; Potin, Philippe; Pineau, Charles			Peptimapper: proteogenomics workflow for the expert annotation of eukaryotic genomes	BMC GENOMICS			English	Article						Bioinformatics; Genome annotation; Peptide sequence tag; Proteogenomics; Proteomics; Tandem mass spectrometry	MASS-SPECTROMETRY DATA; ECTOCARPUS GENOME; DATABASE SEARCH; BROWN; IDENTIFICATION; ORGANISMS; DISCOVERY; SEQUENCES; PEPTIDES; PROTEINS	BackgroundAccurate structural annotation of genomes is still a challenge, despite the progress made over the past decade. The prediction of gene structure remains difficult, especially for eukaryotic species, and is often erroneous and incomplete. We used a proteogenomics strategy, taking advantage of the combination of proteomics datasets and bioinformatics tools, to identify novel protein coding-genes and splice isoforms, assign correct start sites, and validate predicted exons and genes.ResultsOur proteogenomics workflow, Peptimapper, was applied to the genome annotation of Ectocarpus sp., a key reference genome for both the brown algal lineage and stramenopiles. We generated proteomics data from various life cycle stages of Ectocarpus sp. strains and sub-cellular fractions using a shotgun approach. First, we directly generated peptide sequence tags (PSTs) from the proteomics data. Second, we mapped PSTs onto the translated genomic sequence. Closely located hits (i.e., PSTs locations on the genome) were then clustered to detect potential coding regions based on parameters optimized for the organism. Third, we evaluated each cluster and compared it to gene predictions from existing conventional genome annotation approaches. Finally, we integrated cluster locations into GFF files to use a genome viewer. We identified two potential novel genes, a ribosomal protein L22 and an aryl sulfotransferase and corrected the gene structure of a dihydrolipoamide acetyltransferase. We experimentally validated the results by RT-PCR and using transcriptomics data.ConclusionsPeptimapper is a complementary tool for the expert annotation of genomes. It is suitable for any organism and is distributed through a Docker image available on two public bioinformatics docker repositories: Docker Hub and BioShaDock. This workflow is also accessible through the Galaxy framework and for use by non-computer scientists at https://galaxy.protim.eu.Data are available via ProteomeXchange under identifier PXD010618.	[Guillot, Laetitia; Com, Emmanuelle; Lavigne, Regis; Pineau, Charles] Univ Rennes, INSERM, EHESP, Irset,UMR S 1085, F-35042 Rennes, France; [Delage, Ludovic; Ritter, Andres; Marie, Dominique; Potin, Philippe] Sorbonne Univ, UPMC, CNRS, UMR 8227,Integrat Biol Marine Models,Biol Stn, CS 90074, F-29688 Roscoff, France; [Viari, Alain] INRIA Grenoble Rhone Alpes, F-38330 Montbonnot St Martin, France; [Vandenbrouck, Yves] Univ Grenoble Alpes, CEA, INSERM, BIG BGE, F-38000 Grenoble, France; [Ritter, Andres] Sorbonne Univ, CNRS, Inst Biol Paris Seine, Lab Computat & Quantitat Biol, F-75005 Paris, France; [Peterlongo, Pierre] Univ Rennes, INRIA, CNRS, IRISA, F-35042 Rennes, France; [Guillot, Laetitia; Com, Emmanuelle; Lavigne, Regis; Pineau, Charles] Univ Rennes, Protim, F-35042 Rennes, France	Pineau, C (corresponding author), Univ Rennes, INSERM, EHESP, Irset,UMR S 1085, F-35042 Rennes, France.; Pineau, C (corresponding author), Univ Rennes, Protim, F-35042 Rennes, France.	laetitia.guillot@univ-rennes1.fr; charles.pineau@inserm.fr	Com, Emmanuelle/AAD-2312-2020; Pineau, Charles/D-9185-2013; VANDENBROUCK, Yves/Q-2675-2017; Com, Emmanuelle/E-8656-2011; , Protim/C-4687-2012	Com, Emmanuelle/0000-0002-7401-817X; Pineau, Charles/0000-0002-7461-5433; VANDENBROUCK, Yves/0000-0002-1292-373X; Com, Emmanuelle/0000-0002-7401-817X; Ritter, Andres/0000-0001-7011-6824; Guillot, Laetitia/0000-0002-9990-0126	French National Research Agency via the investment expenditure programme IDEALG [ANR-10-BTBR-04-02]; Biogenouest, Infrastructures en Biologie Sante et Agronomie (IBiSA); Conseil Regional de BretagneRegion Bretagne	This work was supported by the French National Research Agency via the investment expenditure programme IDEALG (ANR-10-BTBR-04-02). This work was conducted at the PROTIM core facility (https://www.protim.eu) and supported by grants from Biogenouest, Infrastructures en Biologie Sante et Agronomie (IBiSA) and Conseil Regional de Bretagne awarded to C.P.	Afgan E, 2018, NUCLEIC ACIDS RES, V46, pW537, DOI 10.1093/nar/gky379; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Armengaud J, 2017, METHOD ENZYMOL, V585, P201, DOI 10.1016/bs.mie.2016.09.019; Armengaud J, 2014, J PROTEOMICS, V105, P5, DOI 10.1016/j.jprot.2014.01.007; Armengaud J, 2013, PROTEOMICS, V13, P2731, DOI 10.1002/pmic.201200576; Avia K, 2017, SCI REP-UK, V7, DOI 10.1038/srep43241; Bern M, 2007, ANAL CHEM, V79, P1393, DOI 10.1021/ac0617013; Carver T, 2012, BIOINFORMATICS, V28, P464, DOI 10.1093/bioinformatics/btr703; Castellana NE, 2008, P NATL ACAD SCI USA, V105, P21034, DOI 10.1073/pnas.0811066106; Chambers MC, 2017, CANCER RES, V77, pE43, DOI 10.1158/0008-5472.CAN-17-0331; Chapman Brett, 2013, Methods Mol Biol, V1002, P267, DOI 10.1007/978-1-62703-360-2_21; Chocu S, 2014, BIOL REPROD, V91, DOI 10.1095/biolreprod.114.122416; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Com E, 2012, J PROTEOMICS, V75, P3898, DOI 10.1016/j.jprot.2012.04.034; Contreras L, 2008, J PHYCOL, V44, P1315, DOI 10.1111/j.1529-8817.2008.00575.x; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Crappe J, 2015, NUCLEIC ACIDS RES, V43, DOI 10.1093/nar/gku1283; Data KK, 2016, METHODS MOL BIOL, V1410, P77, DOI 10.1007/978-1-4939-3524-6_5; de Groot A, 2009, PLOS GENET, V5, DOI 10.1371/journal.pgen.1000434; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Fan J, 2015, MOL CELL PROTEOMICS, V14, P3087, DOI 10.1074/mcp.O115.048777; Ferro M, 2008, J PROTEOME RES, V7, P1873, DOI 10.1021/pr070415k; Frank A, 2005, ANAL CHEM, V77, P964, DOI 10.1021/ac048788h; Ghali F, 2014, PROTEOMICS, V14, P2731, DOI 10.1002/pmic.201400265; Goecks J, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-8-r86; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Has Canan, 2016, J Integr Bioinform, V13, P16, DOI 10.1515/jib-2016-293; Has C, 2016, J INTEGR BIOINFORMAT, V13, DOI 10.2390/biecoll-jib-2016-293; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Jaffe JD, 2004, PROTEOMICS, V4, P59, DOI 10.1002/pmic.200300511; Jagtap PD, 2014, J PROTEOME RES, V13, P5898, DOI 10.1021/pr500812t; Kalume DE, 2005, BMC GENOMICS, V6, DOI 10.1186/1471-2164-6-128; Kim H, 2015, J PROTEOME RES, V14, P2784, DOI 10.1021/acs.jproteome.5b00047; Krug K, 2011, MOL BIOSYST, V7, P284, DOI 10.1039/c0mb00168f; Kumar D, 2016, MOL CELL PROTEOMICS, V15, P329, DOI 10.1074/mcp.M114.047126; Kuster B, 2001, PROTEOMICS, V1, P641; Lavigne R, 2012, MOL CELL PROTEOMICS, V11, DOI 10.1074/mcp.M111.012682; Li YX, 2016, J PROTEOME RES, V15, P2309, DOI 10.1021/acs.jproteome.6b00344; Lipinska AP, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140535; Lipinska AP, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-909; MANN M, 1994, ANAL CHEM, V66, P4390, DOI 10.1021/ac00096a002; Menschaert G, 2017, MASS SPECTROM REV, V36, P584, DOI 10.1002/mas.21483; Menschaert G, 2010, J PROTEOME RES, V9, P990, DOI 10.1021/pr900885k; Moreews Francois, 2015, F1000Res, V4, P1443, DOI 10.12688/f1000research.7536.1; Muller SA, 2013, J PROTEOMICS, V86, P27, DOI 10.1016/j.jprot.2013.04.036; Nagaraj SH, 2015, J PROTEOME RES, V14, P2255, DOI 10.1021/acs.jproteome.5b00029; Nanduri B, 2010, METHODS MOL BIOL, V604, P137, DOI 10.1007/978-1-60761-444-9_10; Nesvizhskii AI, 2014, NAT METHODS, V11, P1114, DOI [10.1038/NMETH.3144, 10.1038/nmeth.3144]; Pandey A, 2014, PROTEOMICS, V14, P2631, DOI 10.1002/pmic.201470173; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Potgieter MG, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.00427; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; Risk BA, 2013, J PROTEOME RES, V12, P3019, DOI 10.1021/pr400208w; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Ruggles KV, 2017, MOL CELL PROTEOMICS, V16, P959, DOI 10.1074/mcp.MR117.000024; Sajulga R, 2018, J PROTEOME RES, V17, P4329, DOI 10.1021/acs.jproteome.8b00404; Sanders WS, 2011, BMC BIOINFORMATICS, V12, DOI 10.1186/1471-2105-12-115; Shevchenko A, 2001, ANAL CHEM, V73, P1917, DOI 10.1021/ac0013709; Sheynkman GM, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-703; Venter E, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0027587; Vizcaino JA, 2016, NUCLEIC ACIDS RES, V44, pD447, DOI 10.1093/nar/gkv1145; Wright JC, 2016, NAT COMMUN, V7, DOI 10.1038/ncomms11778; YATES JR, 1995, ANAL CHEM, V67, P3202, DOI 10.1021/ac00114a016; Zhu YA, 2018, NAT COMMUN, V9, DOI 10.1038/s41467-018-03785-w	67	2	2	0	7	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2164			BMC GENOMICS	BMC Genomics	JAN 17	2019	20								56	10.1186/s12864-019-5431-9			19	Biotechnology & Applied Microbiology; Genetics & Heredity	Biotechnology & Applied Microbiology; Genetics & Heredity	HH8NV	WOS:000455989800003	30654742	DOAJ Gold, Green Published			2021-04-07	
J	Arun, A; Coelho, SM; Peters, AF; Bourdareau, S; Peres, L; Scornet, D; Section, MS; Lipinska, AP; Yao, HQ; Godfroy, O; Montecinos, GJ; Avia, K; Macaisne, N; Troadec, C; Bendahmane, A; Cock, JM				Arun, Alok; Coelho, Susana M.; Peters, Akira F.; Bourdareau, Simon; Peres, Laurent; Scornet, Delphine; Section, Martina Strittmatter; Lipinska, Agnieszka P.; Yao, Haiqin; Godfroy, Olivier; Montecinos, Gabriel J.; Avia, Komlan; Macaisne, Nicolas; Troadec, Christelle; Bendahmane, Abdelhafid; Cock, J. Mark			Convergent recruitment of TALE homeodomain life cycle regulators to direct sporophyte development in land plants and brown algae	ELIFE			English	Article							PHYSCOMITRELLA-PATENS; SEXUAL DEVELOPMENT; KNOX GENES; PROTEINS; ECTOCARPUS; EVOLUTION; GENOME; MULTICELLULARITY; DIMERIZATION; ANNOTATION	Three amino acid loop extension homeodomain transcription factors (TALE HD TFs) act as life cycle regulators in green algae and land plants. In mosses these regulators are required for the deployment of the sporophyte developmental program. We demonstrate that mutations in either of two TALE HD TF genes, OUROBOROS or SAMSARA, in the brown alga Ectocarpus result in conversion of the sporophyte generation into a gametophyte. The OUROBOROS and SAMSARA proteins heterodimerise in a similar manner to TALE HD TF life cycle regulators in the green lineage. These observations demonstrate that TALE-HD-TF-based life cycle regulation systems have an extremely ancient origin, and that these systems have been independently recruited to regulate sporophyte developmental programs in at least two different complex multicellular eukaryotic supergroups, Archaeplastida and Chromalveolata.	[Arun, Alok; Coelho, Susana M.; Bourdareau, Simon; Peres, Laurent; Scornet, Delphine; Section, Martina Strittmatter; Lipinska, Agnieszka P.; Yao, Haiqin; Godfroy, Olivier; Montecinos, Gabriel J.] Sorbonne Univ, CNRS, Algal Genet Grp, Integrat Biol Marine Models LBI2M,SBR, Roscoff, France; [Peters, Akira F.] Bezhin Rosko, Santec, France; [Troadec, Christelle; Bendahmane, Abdelhafid] Univ Paris Sud, CNRS, INRA, Inst Plant Sci Paris Saclay IPS2, Orsay, France; [Arun, Alok; Coelho, Susana M.] Inter Amer Univ Puerto Rico, Dept Sci & Technol, Inst Sustainable Biotechnol, Barranquitas Campus, Barranquitas, PR USA; [Section, Martina Strittmatter] UPMC Univ Paris 06, Sorbonne Univ, Adaptat & Divers Marine Environm, Stn Biol Roscoff,CNRS,UMR 7144, Roscoff, France; [Avia, Komlan] Univ Bourgogne Franche Comte, Univ Bourgogne, INRA, Agroecol,AgroSup Dijon, Dijon, France; [Macaisne, Nicolas] Univ Pittsburgh, Sch Med, Magee Womens Res Inst, Pittsburgh, PA USA		cock@sb-roscoff.fr	Avia, Komlan/E-6850-2015; Coelho, Susana/ABH-8166-2020; Arun, Alok/AAJ-7923-2020	Avia, Komlan/0000-0001-6212-6774; Cock, J. Mark/0000-0002-2650-0383; Macaisne, Nicolas/0000-0002-0109-9845; Bourdareau, Simon/0000-0001-9150-5327; PERES, Laurent/0000-0001-6016-4785; Arun, Alok/0000-0003-4666-9802; Strittmatter, Martina/0000-0002-1258-9751	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [ANR-10-BLAN-1727, ANR-10-BTBR-04-01, ANR-10-LABX-40]; Interreg Program France (Channel)-England; University Pierre and Marie Curie; European Research CouncilEuropean Research Council (ERC)European Commission [638240]; European Commission European Erasmus Mundus program; China Scholarship CouncilChina Scholarship Council; European Research Council ERC-SEXYPARTHEuropean Research Council (ERC)	Centre National de la Recherche Scientifique Alok Arun Susana M Coelho Akira F Peters Simon Bourdareau Laurent Peres Delphine Scornet Martina Strittmatter Agnieszka P Lipinska Haiqin Yao Olivier Godfroy Gabriel J Montecinos Komlan Avia Nicolas Macaisne Christelle Troadec Abdelhafid Bendahmane J Mark Cock; Agence Nationale de la Recherche ANR-10-BLAN-1727 J Mark Cock; Interreg Program France (Channel)-England Marinexus J Mark Cock; University Pierre and Marie Curie Alok Arun Susana M Coelho Akira F Peters Simon Bourdareau Laurent Peres Delphine Scornet Martina Strittmatter Agnieszka P Lipinska Haiqin Yao Olivier Godfroy Gabriel J Montecinos Komlan Avia Nicolas Macaisne J Mark Cock; European Research Council 638240 Susana M Coelho; European Commission European Erasmus Mundus program J Mark Cock; China Scholarship Council J Mark Cock; Agence Nationale de la Recherche ANR-10-BTBR-04-01 J Mark Cock; Agence Nationale de la Recherche ANR-10-LABX-40 Abdelhafid Bendahmane; European Research Council ERC-SEXYPARTH Abdelhafid Bendahmane; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.	Abeel T, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gkr995; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Anders S, 2015, BIOINFORMATICS, V31, P166, DOI 10.1093/bioinformatics/btu638; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Arun A, 2013, NEW PHYTOL, V197, P503, DOI 10.1111/nph.12007; BANHAM AH, 1995, PLANT CELL, V7, P773, DOI 10.1105/tpc.7.6.773; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Bowman JL, 2016, ANNU REV GENET, V50, P133, DOI 10.1146/annurev-genet-120215-035227; Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; Buchan DWA, 2013, NUCLEIC ACIDS RES, V41, pW349, DOI 10.1093/nar/gkt381; Burglin TR, 1997, NUCLEIC ACIDS RES, V25, P4173, DOI 10.1093/nar/25.21.4173; Champagne CEM, 2001, NEW PHYTOL, V150, P23, DOI 10.1046/j.1469-8137.2001.00076.x; Cock JM, 2015, ADV MAR GENOMICS, V2, P153, DOI 10.1007/978-94-017-9642-2_8; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Conesa Ana, 2008, Int J Plant Genomics, V2008, P619832, DOI 10.1155/2008/619832; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Eme L, 2014, CSH PERSPECT BIOL, V6, DOI 10.1101/cshperspect.a016139; Furumizu C, 2015, PLOS GENET, V11, DOI 10.1371/journal.pgen.1004980; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Hedgethorne K, 2017, SCI ADV, V3, DOI 10.1126/sciadv.1602937; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Horst NA, 2016, NAT PLANTS, V2, DOI [10.1038/NPLANTS.2015.209, 10.1038/nplants.2015.209]; Hull CM, 2005, EUKARYOT CELL, V4, P526, DOI 10.1128/EC.4.3.526-535.2005; Joo S, 2018, BMC BIOL, V16, DOI 10.1186/s12915-018-0605-5; KAMPER J, 1995, CELL, V81, P73, DOI 10.1016/0092-8674(95)90372-0; Kim D, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-4-r36; Lee JH, 2008, CELL, V133, P829, DOI 10.1016/j.cell.2008.04.028; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; Macaisne N, 2017, DEVELOPMENT, V144, P409, DOI 10.1242/dev.141523; Matasci N, 2014, GIGASCIENCE, V3, DOI 10.1186/2047-217X-3-17; Mukherjee K, 2009, MOL BIOL EVOL, V26, P2775, DOI 10.1093/molbev/msp201; Nagy LG, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms5471; NASMYTH K, 1987, SCIENCE, V237, P1162, DOI 10.1126/science.3306917; Niklas KJ, 2015, FRONT CELL DEV BIOL, V3, DOI 10.3389/fcell.2015.00008; Nishitsuji K, 2016, DNA RES, V23, P561, DOI 10.1093/dnares/dsw039; Ortiz-Ramirez C, 2017, NATURE, V549, P91, DOI 10.1038/nature23478; Perrin N, 2012, EVOLUTION, V66, P947, DOI 10.1111/j.1558-5646.2011.01562.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Ploner A., 2015, HEATPLUS HEATMAPS RO; Sakakibara K, 2008, EVOL DEV, V10, P555, DOI 10.1111/j.1525-142X.2008.00271.x; Sakakibara K, 2013, SCIENCE, V339, P1067, DOI 10.1126/science.1230082; Singer SD, 2007, PLANT CELL REP, V26, P1155, DOI 10.1007/s00299-007-0312-0; Stamatakis A., 2015, CURR PROTOC BIOINFOR, V51, DOI DOI 10.1002/0471250953.BI0614S51; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; van Heeckeren WJ, 1998, MOL CELL BIOL, V18, P7317, DOI 10.1128/MCB.18.12.7317; VOS P, 1995, NUCLEIC ACIDS RES, V23, P4407, DOI 10.1093/nar/23.21.4407; WOOTTON JC, 1994, COMPUT CHEM, V18, P269, DOI 10.1016/0097-8485(94)85023-2; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zhang T, 2012, J BIOMOL STRUCT DYN, V29, P799, DOI 10.1080/073911012010525022	53	17	17	3	10	ELIFE SCIENCES PUBLICATIONS LTD	CAMBRIDGE	SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND	2050-084X			ELIFE	eLife	JAN 15	2019	8								e43101	10.7554/eLife.43101			25	Biology	Life Sciences & Biomedicine - Other Topics	HK7BI	WOS:000458141200001	30644818	DOAJ Gold, Green Published			2021-04-07	
J	Boundir, Y; Hasni, M; Rafik, F; Sabri, H; Bahammou, N; Cheggour, M; Achtak, H; Cherifi, O				Boundir, Y.; Hasni, M.; Rafik, F.; Sabri, H.; Bahammou, N.; Cheggour, M.; Achtak, H.; Cherifi, O.			FIRST STUDY OF THE ECOLOGICAL STATUS IN THE ATLANTIC COAST OF MOROCCO USING THE BROWN SEAWEED CYSTOSEIRA TAMARISCIFOLIA	APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH			English	Article						environmental pollution; heavy metals; physicochemical parameters; algal physiology	ECTOCARPUS-SILICULOSUS; METAL CONTAMINATION; AMBIENT SALINITY; POLLUTION; EUTROPHICATION; COPPER; ALGAE; RESPONSES; PROLINE; IMPACTS	This study is a first attempt to evaluate the toxic effects of prominent aquatic pollutants (nutrients and toxic metals) on the brown seaweed Bushy Rainbow Wrack (Cystoseira tamariscifolia) physiology along the Atlantic coast of Morocco. The physicochemistry (nutrients) of seawater, toxic metals (Chromium, Lead, Copper and Cadmium) and physiological parameters (Chlorophyll contents, Proline, Glycinebetaine and Total Phenolic Compounds) of the brown macroalgae Bushy Rainbow Wrack were studied in order to assess the pollution degree of 8 coastal areas. The results show that the toxic metal contents of Bushy Rainbow Wrack (especially Cadmium) and the concentration of phosphorus are correlated with stress physiological parameters, and inversely correlated with pigment contents. It shows that while these brown algae exist in the less polluted areas, their physiology is significantly affected. However, in the highly polluted areas, this brown seaweed disappears. Thus, this specie could be used for monitoring the pollution degree in coastal areas.	[Boundir, Y.; Sabri, H.; Bahammou, N.; Cherifi, O.] Cadi Ayyad Univ, Lab Hydrobiol Ecotoxicol Sanitat & Global Changes, Fac Sci Semlalia, Bd Prince My Abdellah,POB 2390, Marrakech 40000, Morocco; [Boundir, Y.; Rafik, F.; Achtak, H.] Cadi Ayyad Univ, Polydisciplinary Fac, Environm & Hlth Team, Route Sidi Bouzid POB 4162 Ave Mohamed Belkhadir, Safi 46000, Morocco; [Boundir, Y.; Sabri, H.; Bahammou, N.; Cherifi, O.] Cadi Ayyad Univ, Natl Ctr Studies & Res Water & Energy CNEREE, Ave Abdelkrim Khattabi,POB 511, Marrakech 40000, Morocco; [Hasni, M.] Ibn Zohr Univ, Fac Sci, POB 8106, Agadir 80000, Morocco; [Hasni, M.; Cheggour, M.] Cadi Ayyad Univ, Dept Biol, Ecol Unit, Ecole Normale Super, Hay Hassani Route Essaouira,POB 2400, Marrakech 40000, Morocco	Boundir, Y (corresponding author), Cadi Ayyad Univ, Lab Hydrobiol Ecotoxicol Sanitat & Global Changes, Fac Sci Semlalia, Bd Prince My Abdellah,POB 2390, Marrakech 40000, Morocco.; Boundir, Y (corresponding author), Cadi Ayyad Univ, Polydisciplinary Fac, Environm & Hlth Team, Route Sidi Bouzid POB 4162 Ave Mohamed Belkhadir, Safi 46000, Morocco.; Boundir, Y (corresponding author), Cadi Ayyad Univ, Natl Ctr Studies & Res Water & Energy CNEREE, Ave Abdelkrim Khattabi,POB 511, Marrakech 40000, Morocco.	younes.boundir@ced.uca.ma	BAHAMMOU, Nadia/ABF-8556-2020; Boundir, Younes/AAL-3677-2020	BAHAMMOU, Nadia/0000-0001-8857-3812; Boundir, Younes/0000-0002-5066-3019; CHERIFI, Ouafa/0000-0003-2635-4686			Abdel Latef A. A. H., 2015, AUSTIN J PLANT BIOL, V1, P1004; Akcali I, 2011, MAR POLLUT BULL, V62, P637, DOI 10.1016/j.marpolbul.2010.12.021; Al-Masri MS, 2003, J ENVIRON RADIOACTIV, V67, P157, DOI 10.1016/S0265-931X(02)00177-7; ALIA, 1991, J PLANT PHYSIOL, V138, P554, DOI 10.1016/S0176-1617(11)80240-3; ANBAZHAGAN M, 1988, J PLANT PHYSIOL, V133, P122, DOI 10.1016/S0176-1617(88)80098-1; [Anonymous], 2000, T900152 AFNOR NF; [Anonymous], 1998, 119051 NF EN ISO; [Anonymous], 1975, T90012 AFNOR NF; [Anonymous], 2005, 6878 NF EN ISO; [Anonymous], 2005, T90022 AFNOR NF; AustralianJointRegister, 2018, ANN REPORT; Baez JC, 2005, BOT MAR, V48, P30, DOI 10.1515/BOT.2005.012; BALLESTEROS E, 1984, Collectanea Botanica (Barcelona), V15, P69; Ballesteros E, 2007, MAR POLLUT BULL, V55, P172, DOI 10.1016/j.marpolbul.2006.08.038; Bermejo R, 2018, SCI REPORTS, V8, P1; Blinda M., 2013, Bulletin de l'Institut Scientifique: Section Sciences de la Vie, V35, P43; Caliceti M, 2002, CHEMOSPHERE, V47, P443, DOI 10.1016/S0045-6535(01)00292-2; Celis-Pla PSM, 2014, AQUAT BIOL, V22, P227, DOI 10.3354/ab00573; Celis-Pla PSM, 2016, MAR ENVIRON RES, V115, P89, DOI 10.1016/j.marenvres.2015.11.014; CHERIFI O, 2018, SMETOX J, V1, P53; Claudet J, 2010, BIOL CONSERV, V143, P2195, DOI 10.1016/j.biocon.2010.06.004; Connan S, 2011, AQUAT TOXICOL, V104, P94, DOI 10.1016/j.aquatox.2011.03.015; Connan S, 2011, AQUAT TOXICOL, V104, P1, DOI 10.1016/j.aquatox.2011.03.016; Cormaci M, 1999, DEV HYDROBIOLOGY; DAYTON PK, 1975, ECOL MONOGR, V45, P137, DOI 10.2307/1942404; Essedaoui A., 2001, ACTES I AGRONOMIQUE, V21, P17; FAO, 2006, FAOUTFMOR019MOR; Fatma T, 2007, J APPL PHYCOL, V19, P625, DOI 10.1007/s10811-007-9195-2; Ferreira JG, 2011, ESTUAR COAST SHELF S, V93, P117, DOI 10.1016/j.ecss.2011.03.014; Ferssiwi A., 2004, J RECHERCHE OCEANOGR, V29, P59; Fleurence J, 2004, SEAWEED PROTEINS; Goumri Meryenn, 2018, AACL Bioflux, V11, P1193; GRIEVE CM, 1983, PLANT SOIL, V70, P303, DOI 10.1007/BF02374789; Guiry, 2019, ALGAEBASE; Haas P, 1933, ANN BOT-LONDON, V47, P55, DOI 10.1093/oxfordjournals.aob.a090377; JEFFREY SW, 1975, BIOCHEM PHYSIOL PFL, V167, P191, DOI 10.1016/s0015-3796(17)30778-3; JONES A, 1997, ENV BIOL; Kaimoussi A, 2001, CR ACAD SCI II A, V333, P337, DOI 10.1016/S1251-8050(01)01647-0; Koivikko R, 2005, J CHEM ECOL, V31, P195, DOI 10.1007/s10886-005-0984-2; LICHTENTHALER HK, 1987, METHOD ENZYMOL, V148, P350; McGlathery KJ, 2007, MAR ECOL PROG SER, V348, P1, DOI 10.3354/meps07132; Mineur F, 2015, J SEA RES, V98, P91, DOI 10.1016/j.seares.2014.11.004; MONNEVEUX P, 1986, AGRONOMIE, V6, P583, DOI 10.1051/agro:19860611; Moussa Hanaa, 2018, Acta Botanica Malacitana, V43, P91, DOI 10.24310/abm.v43i0.4966; Munda I., 1982, ACTA ADRIAT, V23, P329; Munday PL, 2013, ECOL LETT, V16, P1488, DOI 10.1111/ele.12185; Nielsen HD, 2010, MAR POLLUT BULL, V60, P710, DOI 10.1016/j.marpolbul.2009.11.025; NIXON SW, 1995, OPHELIA, V41, P199, DOI 10.1080/00785236.1995.10422044; Pereira RC, 1999, BOT MAR, V42, P441, DOI 10.1515/BOT.1999.051; RIBERA MA, 1992, BOT MAR, V35, P109, DOI 10.1515/botm.1992.35.2.109; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; SABRI H, 2017, J MAT ENV SCI, V8, P857; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Sales M, 2009, ESTUAR COAST SHELF S, V84, P476, DOI 10.1016/j.ecss.2009.07.013; Scavia D, 2006, BIOGEOCHEMISTRY, V79, P187, DOI 10.1007/s10533-006-9011-0; Schintu M, 2010, ENVIRON MONIT ASSESS, V167, P653, DOI 10.1007/s10661-009-1081-8; Schramm W, 1999, DEV HYDROBIOLOGY; Sverdrup H. U, 1943, PHYSIOL ZOOL, V16, P322; TAGA MS, 1984, J AM OIL CHEM SOC, V61, P928, DOI 10.1007/BF02542169; Taskin E., 2012, MEDITERRANEAN CYSTOS; Thibaut T, 2005, MAR POLLUT BULL, V50, P1472, DOI 10.1016/j.marpolbul.2005.06.014; Topcuoglu S, 2003, CHEMOSPHERE, V52, P1683, DOI 10.1016/S0045-6535(03)00301-1; Walker DI, 1998, BOT MAR, V41, P105, DOI 10.1515/botm.1998.41.1-6.105	63	1	1	3	7	CORVINUS UNIV BUDAPEST	BUDAPEST	VILLANYI UT 29/43, BUDAPEST, H-1118, HUNGARY	1589-1623	1785-0037		APPL ECOL ENV RES	Appl. Ecol. Environ. Res.		2019	17	6					14315	14331		10.15666/aeer/1706_1431514331			17	Ecology; Environmental Sciences	Environmental Sciences & Ecology	JZ6XS	WOS:000505251300112		Bronze			2021-04-07	
J	Rabille, H; Billoud, B; Tesson, B; Le Panse, S; Rolland, E; Charrier, B				Rabille, Herve; Billoud, Bernard; Tesson, Benoit; Le Panse, Sophie; Rolland, Elodie; Charrier, Benedicte			The brown algal mode of tip growth: Keeping stress under control	PLOS BIOLOGY			English	Article							ECTOCARPUS-SILICULOSUS ECTOCARPALES; POLLEN-TUBE GROWTH; CELL-WALL; EXTRACELLULAR-MATRIX; TURGOR PRESSURE; POLAR GROWTH; EVOLUTION; MORPHOGENESIS; METABOLISM; DIVERSITY	Tip growth has been studied in pollen tubes, root hairs, and fungal and oomycete hyphae and is the most widely distributed unidirectional growth process on the planet. It ensures spatial colonization, nutrient predation, fertilization, and symbiosis with growth speeds of up to 800 mu m h(-1). Although turgor-driven growth is intuitively conceivable, a closer examination of the physical processes at work in tip growth raises a paradox: growth occurs where biophysical forces are low, because of the increase in curvature in the tip. All tip-growing cells studied so far rely on the modulation of cell wall extensibility via the polarized excretion of cell wall-loosening compounds at the tip. Here, we used a series of quantitative measurements at the cellular level and a biophysical simulation approach to show that the brown alga Ectocarpus has an original tip-growth mechanism. In this alga, the establishment of a steep gradient in cell wall thickness can compensate for the variation in tip curvature, thereby modulating wall stress within the tip cell. Bootstrap analyses support the robustness of the process, and experiments with fluorescence recovery after photobleaching (FRAP) confirmed the active vesicle trafficking in the shanks of the apical cell, as inferred from the model. In response to auxin, biophysical measurements change in agreement with the model. Although we cannot strictly exclude the involvement of a gradient in mechanical properties in Ectocarpus morphogenesis, the viscoplastic model of cell wall mechanics strongly suggests that brown algae have evolved an alternative strategy of tip growth. This strategy is largely based on the control of cell wall thickness rather than fluctuations in cell wall mechanical properties.	[Rabille, Herve; Billoud, Bernard; Rolland, Elodie; Charrier, Benedicte] Sorbonne Univ, CNRS, Morphogenesis Macro Algae, UMR8227,Stn Biol, Roscoff, France; [Tesson, Benoit] Univ Calif San Diego, Scripps Inst Oceanog, San Diego, CA 92103 USA; [Le Panse, Sophie] Sorbonne Univ, MerImage Platform, FR2424, CNRS,Stn Biol, Roscoff, France	Charrier, B (corresponding author), Sorbonne Univ, CNRS, Morphogenesis Macro Algae, UMR8227,Stn Biol, Roscoff, France.	Benedicte.Charrier@sb-roscoff.fr		Billoud, Bernard/0000-0002-5140-8087; Charrier, Benedicte/0000-0001-5721-1640	Region Bretagne (ARED "ECTOTIP"); France BioImagingFrench National Research Agency (ANR) [ANR-10-INBS-04-01]; Labex "Saclay Plant Science [ANR-11-IDEX-0003-02]	Region Bretagne (ARED "ECTOTIP"). Received by HR. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. France BioImaging (grant number ANR-10-INBS-04-01). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Labex "Saclay Plant Science (grant number ANR-11-IDEX-0003-02). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Benkert R, 1997, PROTOPLASMA, V198, P1, DOI 10.1007/BF01282125; Bolte S, 2004, J MICROSC-OXFORD, V214, P159, DOI 10.1111/j.0022-2720.2004.01348.x; Bove J, 2008, PLANT PHYSIOL, V147, P1646, DOI 10.1104/pp.108.120212; Buck DK, 2013, POV RAY PERSISTENCE, DOI [10.1105/tpc.109.069260, DOI 10.1105/TPC.109.069260]; Butterfield NJ, 2000, PALEOBIOLOGY, V26, P386, DOI 10.1666/0094-8373(2000)026<0386:BPNGNS>2.0.CO;2; Cai G, 2011, PLANT PHYSIOL, V155, P1169, DOI 10.1104/pp.110.171371; Campas O, 2012, AM J BOT, V99, P1577, DOI 10.3732/ajb.1200087; Castle ES, 1937, J CELL COMPAR PHYSL, V10, P113, DOI 10.1002/jcp.1030100110; Cava F, 2013, CURR OPIN MICROBIOL, V16, P731, DOI 10.1016/j.mib.2013.09.004; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Charrier B, 2019, TRENDS PLANT SCI, V24, P130, DOI 10.1016/j.tplants.2018.10.013; Chebli Y, 2013, MOL PLANT, V6, P1037, DOI 10.1093/mp/sst073; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cosgrove DJ, 2018, PLANT PHYSIOL, V176, P16, DOI 10.1104/pp.17.01541; Cosgrove DJ, 2016, J EXP BOT, V67, P463, DOI 10.1093/jxb/erv511; Cosgrove DJ, 2005, NAT REV MOL CELL BIO, V6, P850, DOI 10.1038/nrm1746; Davi V, 2018, DEV CELL, V45, P170, DOI 10.1016/j.devcel.2018.03.022; Davidson LA, 2017, PHILOS T R SOC B, V372, DOI 10.1098/rstb.2015.0516; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; DERKSEN J, 1995, PROTOPLASMA, V188, P267, DOI 10.1007/BF01280379; Domozych David S, 2013, Plants (Basel), V2, P148, DOI 10.3390/plants2010148; Dumais J, 2006, INT J DEV BIOL, V50, P209, DOI 10.1387/ijdb.052066jd; Dunn CW, 2014, ANNU REV ECOL EVOL S, V45, P371, DOI 10.1146/annurev-ecolsys-120213-091627; Fayant P, 2010, PLANT CELL, V22, P2579, DOI 10.1105/tpc.110.075754; Geitmann A, 2006, PLANT CELL MONOGR, V3, P177, DOI 10.1007/7089_049; Geitmann A, 2009, TRENDS PLANT SCI, V14, P467, DOI 10.1016/j.tplants.2009.07.006; Goriely Alain, 2008, Fungal Biology Reviews, V22, P77, DOI 10.1016/j.fbr.2008.05.001; Hamant O, 2017, BMC BIOL, V15, DOI 10.1186/s12915-017-0403-5; Harold RL, 1996, PROTOPLASMA, V191, P105, DOI 10.1007/BF01280830; Heath I.B., 1990, TIP GROWTH PLANT FUN; Hill R., 1998, MATH THEORY PLASTICI; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; Kenrick P, 1997, NATURE, V389, P33, DOI 10.1038/37918; LANCELLE SA, 1992, PROTOPLASMA, V167, P215, DOI 10.1007/BF01403385; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail Aude, 2013, Methods Mol Biol, V959, P323, DOI 10.1007/978-1-62703-221-6_22; Le Bail A, 2010, PLANT PHYSIOL, V153, P128, DOI 10.1104/pp.109.149708; Leliaert F, 2012, CRIT REV PLANT SCI, V31, P1, DOI 10.1080/07352689.2011.615705; LOCKHART JA, 1965, J THEOR BIOL, V8, P264, DOI 10.1016/0022-5193(65)90077-9; McHugh DJ, 2003, GUIDE SEAWEED IND, P404, DOI [10.1666/0094-8373(2000), DOI 10.1666/0094-8373(2000)]; McKenna ST, 2009, PLANT CELL, V21, P3026, DOI 10.1105/tpc.109.069260; Menand B, 2007, J EXP BOT, V58, P1843, DOI 10.1093/jxb/erm047; Meyers MA, 2008, MECH BEHAV MAT, DOI [10.1146/annurev-arplant-042110-103809, DOI 10.1146/ANNUREV-ARPLANT-042110-103809]; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Mirabet V, 2011, ANNU REV PLANT BIOL, V62, P365, DOI 10.1146/annurev-arplant-042110-103852; Nagasato C, 2010, PLANTA, V232, P287, DOI 10.1007/s00425-010-1188-8; NAKAHORI K, 1991, PLANT CELL PHYSIOL, V32, P121; Ortega JKE, 2017, SCI REP-UK, V7, DOI 10.1038/s41598-017-03002-6; Park YB, 2012, PLANT PHYSIOL, V158, P465, DOI 10.1104/pp.111.189779; Parre E, 2005, PLANTA, V220, P582, DOI 10.1007/s00425-004-1368-5; Parton RM, 2001, J CELL SCI, V114, P2685; PASSIOURA JB, 1992, AUST J PLANT PHYSIOL, V19, P565, DOI 10.1071/PP9920565; Peaucelle A, 2011, CURR BIOL, V21, P1720, DOI 10.1016/j.cub.2011.08.057; Phair RD, 2004, METHOD ENZYMOL, V375, P393; Ponce NMA, 2007, PHYCOLOGIA, V46, P675, DOI 10.2216/06-102.1; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Proseus TE, 1999, PLANT PHYSIOL, V119, P775, DOI 10.1104/pp.119.2.775; Proseus TE, 2007, J EXP BOT, V58, P4283, DOI 10.1093/jxb/erm318; R Core Team, 2017, R LANG ENV STAT COMP; Rabille H, 2018, PROTOCOLS MACROALGAE, P349; Riquelme M, 2013, ANNU REV MICROBIOL, V67, P587, DOI 10.1146/annurev-micro-092412-155652; Saint-Marcoux D, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00054; Schindelin J, 2012, NAT METHODS, V9, P676, DOI [10.1038/NMETH.2019, 10.1038/nmeth.2019]; Shamsudhin N, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0168138; Shaw SL, 2000, PLANT PHYSIOL, V124, P959, DOI 10.1104/pp.124.3.959; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Silverman-Gavrila LB, 2002, J CELL SCI, V115, P5013, DOI 10.1242/jcs.00180; Song Y, 2013, DIS MODEL MECH, V6, P404, DOI 10.1242/dmm.009688; Terauchi M, 2016, PLANTA, V244, P361, DOI 10.1007/s00425-016-2516-4; Tesson B, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00471; Toyooka K, 2009, PLANT CELL, V21, P1212, DOI 10.1105/tpc.108.058933; TRINCI APJ, 1975, J GEN MICROBIOL, V91, P355, DOI 10.1099/00221287-91-2-355; Tsekos I, 1999, J PHYCOL, V35, P635, DOI 10.1046/j.1529-8817.1999.3540635.x; van der Walt S, 2011, COMPUT SCI ENG, V13, P22, DOI 10.1109/MCSE.2011.37; van Rossum Jr G, 2011, PYTHON LANGUAGE REFE; von Dassow M, 2001, PLANTA, V213, P659, DOI 10.1007/s004250100538; WRIGHT PJ, 1988, MAR BIOL, V99, P473, DOI 10.1007/BF00392554; Yanagisawa M, 2015, NAT PLANTS, V1, DOI [10.1038/nplants.2015.14, 10.1038/NPLANTS.2015.14]; Yuan S, 1995, CELL RES, V5, P255, DOI 10.1038/cr.1995.24; Zonia L, 2011, TRENDS PLANT SCI, V16, P347, DOI 10.1016/j.tplants.2011.03.009	82	6	6	0	6	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1544-9173	1545-7885		PLOS BIOL	PLoS. Biol.	JAN	2019	17	1							e2005258	10.1371/journal.pbio.2005258			31	Biochemistry & Molecular Biology; Biology	Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics	HK0NH	WOS:000457596000001	30640903	DOAJ Gold, Green Published			2021-04-07	
J	Bogaert, KA; Blommaert, L; Ljung, K; Beeckman, T; De Clerck, O				Bogaert, Kenny A.; Blommaert, Lander; Ljung, Karin; Beeckman, Tom; De Clerck, Olivier			Auxin Function in the Brown Alga Dictyota dichotoma	PLANT PHYSIOLOGY			English	Article							METABOLIC NETWORK; EMBRYO DEVELOPMENT; INDOLEACETIC-ACID; PATTERN-FORMATION; FUCUS-DISTICHUS; P-GLYCOPROTEINS; GENOME REVEALS; TRANSPORT; ESTABLISHMENT; POLARITY	Auxin controls body plan patterning in land plants and has been proposed to play a similar role in the development of brown algae (Phaeophyta) despite their distant evolutionary relationship with land plants. The mechanism of auxin action in brown algae remains controversial because of contradicting conclusions derived from pharmacological studies on Fucus. In this study, we used Dictyota dichotoma as a model system to show that auxin plays a role during the apical-basal patterning of the embryo of brown algae. Indole-3-acetic acid was detectable in D. dichotoma germlings and mature tissue. Although two-celled D. dichotoma zygotes normally develop a rhizoid from one pole and a thallus meristem from the other, addition of exogenous auxins to onecelled embryos affected polarization, and both poles of the spheroidal embryo developed into rhizoids instead. The effect was strongest at lower pH and when variable extrinsic informational cues were applied. 2-[4-(diethylamino)-2-hydroxybenzoyl] benzoic acid, an inhibitor of the ABC-B/multidrugresistance/P-glycoprotein subfamily of transporters in land plants, affected rhizoid formation by increasing rhizoid branching and inducing ectopic rhizoids. An in silico survey of auxin genes suggested that a diverse range of biosynthesis genes and transport genes, such as PIN-LIKES, and the ATP-binding cassette subfamily (ABC-B/multidrugresistance/P-glycoprotein) transporters from land plants have homologs in D. dichotoma and Ectocarpus siliculosus. Together with reports on auxin function in basal lineages of green algae, these results suggest that auxin function predates the divergence between the green and brown lineage and the transition toward land plants.	[Bogaert, Kenny A.; Blommaert, Lander; De Clerck, Olivier] Univ Ghent, Dept Biol, B-9000 Ghent, Belgium; [Ljung, Karin] Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, SE-90183 Umea, Sweden; [Beeckman, Tom] VIB UGent Ctr Plant Syst Biol, B-9052 Ghent, Belgium; [Beeckman, Tom] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium	Bogaert, KA (corresponding author), Univ Ghent, Dept Biol, B-9000 Ghent, Belgium.	kenny.bogaert@ugent.be	Beeckman, Tom/ABA-1164-2020; De Clerck, Olivier/AAU-4295-2020; Ljung, Karin/AAE-8691-2019; Beeckman, Tom/ABI-1703-2020; bogaert, kenny/AAK-6095-2021	Ljung, Karin/0000-0003-2901-189X; Beeckman, Tom/0000-0001-8656-2060; bogaert, kenny/0000-0003-1482-7618	Research Foundation Flanders (FWO PhD fellowship)FWO [1150111N]; European Community's Seventh Framework Program (FP7/2007-2013) [227799-ASSEMBLE]; Swedish Governmental Agency for Innovation Systems (VINNOVA)Vinnova; Swedish Research CouncilSwedish Research CouncilEuropean Commission; EMBRC Belgium - Research Foundation Flanders project [GOH3817N]	This work was supported by the Research Foundation Flanders (FWO PhD fellowship no. 1150111N to K.A.B.) and the European Community's Seventh Framework Program (FP7/2007-2013 under grant agreement no. 227799-ASSEMBLE). This work was also supported by the Swedish Governmental Agency for Innovation Systems (VINNOVA) and the Swedish Research Council (to K.L.) and with infrastructure funded by EMBRC Belgium - Research Foundation Flanders project (GOH3817N to O.D.C.).	ABE H, 1972, AGR BIOL CHEM TOKYO, V36, P2259, DOI 10.1080/00021369.1972.10860553; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Amin SA, 2015, NATURE, V522, P98, DOI 10.1038/nature14488; Andersen SU, 2008, PLANT CELL, V20, P88, DOI 10.1105/tpc.107.054676; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Bailly A, 2008, J BIOL CHEM, V283, P21817, DOI 10.1074/jbc.M709655200; Barbez E, 2012, NATURE, V485, P119, DOI 10.1038/nature11001; Basu S, 2002, PLANT PHYSIOL, V130, P292, DOI 10.1104/pp.004747; BENTRUP FW, 1968, PROTOPLASMA, V65, P25, DOI 10.1007/BF01666369; BERGER F, 1994, PLANT PHYSIOL, V105, P519, DOI 10.1104/pp.105.2.519; Bittner L, 2008, MOL PHYLOGENET EVOL, V49, P211, DOI 10.1016/j.ympev.2008.06.018; Bogaert KA, 2016, J APPL PHYCOL; Bogaert Kenny A, 2013, Methods Mol Biol, V959, P97, DOI 10.1007/978-1-62703-221-6_6; Bouget FY, 2001, CAH BIOL MAR, V42, P101; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Braendle C, 2009, J BIOSCIENCES, V34, P543, DOI 10.1007/s12038-009-0073-8; BRAWLEY SH, 1987, DEV BIOL, V122, P217, DOI 10.1016/0012-1606(87)90347-2; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Chevreux B, 2004, GENOME RES, V14, P1147, DOI 10.1101/gr.1917404; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cooke TJ, 2002, PLANT MOL BIOL, V49, P319, DOI 10.1023/A:1015242627321; Corellou F, 2000, DEV BIOL, V219, P165, DOI 10.1006/dbio.1999.9603; Corellou F, 2001, DEVELOPMENT, V128, P4383; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; DAVIDSON FF, 1950, AM J BOT, V37, P502, DOI 10.2307/2438025; De Clerck O, 2017, NAT PLANTS; De Smet I, 2014, AUXIN ITS ROLE PLANT, P265; De Smet I, 2011, NAT REV MOL CELL BIO, V12, P177, DOI 10.1038/nrm3064; de Visser JAGM, 2007, NAT REV GENET, V8, P139, DOI 10.1038/nrg1985; de Visser JAGM, 2003, EVOLUTION, V57, P1959, DOI 10.1554/02-750R; Debat V, 2001, TRENDS ECOL EVOL, V16, P555, DOI 10.1016/S0169-5347(01)02266-2; Dittami SM, 2014, FRONT GENET, V5, DOI 10.3389/fgene.2014.00241; du Buy HG, 1937, AM J BOT, V24, P609, DOI 10.2307/2436640; EVANS LV, 1991, J PHYCOL, V27, P322, DOI 10.1111/j.0022-3646.1991.00322.x; Feraru E, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00227; Friml J, 2003, NATURE, V426, P147, DOI 10.1038/nature02085; GAILLARD J, 1990, PHYCOLOGIA, V29, P39, DOI 10.2216/i0031-8884-29-1-39.1; Gattuso J.-P., 2010, GUIDE BEST PRACTICES, P41, DOI DOI 10.1016/J.JOMS.2003.10.009; Geisler M, 2006, FEBS LETT, V580, P1094, DOI 10.1016/j.febslet.2005.11.054; Geldner N, 2003, CELL, V112, P219, DOI 10.1016/S0092-8674(03)00003-5; GIBBON BC, 1993, DEV BIOL, V157, P259, DOI 10.1006/dbio.1993.1130; Gil P, 2001, GENE DEV, V15, P1985, DOI 10.1101/gad.905201; GOLDSMITH MHM, 1977, ANNU REV PLANT PHYS, V28, P439, DOI 10.1146/annurev.pp.28.060177.002255; Grones P, 2015, J CELL SCI, V128, P1, DOI 10.1242/jcs.159418; Hable WE, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00690; Hamann T, 1999, DEVELOPMENT, V126, P1387; Harrison PJ, 2005, ALGAL CULT TECH; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Jurado S, 2010, PLANT CELL, V22, P3891, DOI 10.1105/tpc.110.078972; Jurgens G, 2001, EMBO J, V20, P3609, DOI 10.1093/emboj/20.14.3609; Kasahara H, 2016, BIOSCI BIOTECH BIOCH, V80, P34, DOI 10.1080/09168451.2015.1086259; Khasin M, 2017, BIORXIV, V172833; Kim JY, 2010, J BIOL CHEM, V285, P23307, DOI 10.1074/jbc.M110.105981; KLAMBT D, 1992, PHYSIOL PLANTARUM, V85, P537, DOI 10.1111/j.1399-3054.1992.tb05823.x; Klemke I, 1973, PLANT SCI LETT, V1, P315; Lambrecht M, 2000, TRENDS MICROBIOL, V8, P298, DOI 10.1016/S0966-842X(00)01732-7; Le Bail A, 2010, PLANT PHYSIOL, V153, P128, DOI 10.1104/pp.109.149708; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Leyser O, 2018, PLANT PHYSIOL, V176, P465, DOI 10.1104/pp.17.00765; Ljung K, 2013, DEVELOPMENT, V140, P943, DOI 10.1242/dev.086363; Love J, 1997, PLANT PHYSIOL, V115, P249, DOI 10.1104/pp.115.1.249; Lu YD, 2015, TRENDS PLANT SCI, V20, P273, DOI 10.1016/j.tplants.2015.01.006; Luschnig C, 2001, CURR BIOL, V11, pR831, DOI 10.1016/S0960-9822(01)00497-3; Mano Y, 2012, J EXP BOT, V63, P2853, DOI 10.1093/jxb/ers091; MARTIN HV, 1987, PLANT PHYSIOL, V83, P262, DOI 10.1104/pp.83.2.262; Mashiguchi K, 2011, P NATL ACAD SCI USA, V108, P18512, DOI 10.1073/pnas.1108434108; Mazur H, 2001, J APPL PHYCOL, V13, P35, DOI 10.1023/A:1008199409953; Murphy A, 1999, PLANT PHYSIOL BIOCH, V37, P413, DOI 10.1016/S0981-9428(99)80047-3; Mutte SK, 2018, ELIFE, V7, DOI 10.7554/eLife.33399; Nijhout HF, 2002, BIOESSAYS, V24, P553, DOI 10.1002/bies.10093; NOVOTNY AM, 1974, DEV BIOL, V40, P162, DOI 10.1016/0012-1606(74)90116-X; Ohtaka K, 2017, PLANT PHYSIOL, V174, P1621, DOI 10.1104/pp.17.00274; Paciorek T, 2005, NATURE, V435, P1251, DOI 10.1038/nature03633; Pagnussat GC, 2009, SCIENCE, V324, P1684, DOI 10.1126/science.1167324; Parsons K, 2014, CELL MOL LIFE SCI; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters NT, 2010, CYTOSKELETON, V67, P102, DOI 10.1002/cm.20427; Petricka JJ, 2009, CSH PERSPECT BIOL, V1, DOI 10.1101/cshperspect.a000497; Piotrowska-Niczyporuk A, 2014, PLANT GROWTH REGUL, V73, P57, DOI 10.1007/s10725-013-9867-7; Polevoi V. V., 2003, Ontogenez, V34, P432; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; RAVEN JA, 1975, NEW PHYTOL, V74, P163, DOI 10.1111/j.1469-8137.1975.tb02602.x; Rensing SA, 2008, SCIENCE, V319, P64, DOI 10.1126/science.1150646; Richards S, 1996, GENETICS, V142, P1215; Robinson KR, 1997, DEV BIOL, V187, P125, DOI 10.1006/dbio.1997.8600; Rozov SM, 2014, BIOL B REV, V3, P423; RUBERY PH, 1973, NATURE-NEW BIOL, V244, P285; Salichos L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0018755; Schreiber E, 1935, PLANTA, V24, P266; Shaw SL, 1996, DEVELOPMENT, V122, P2623; Stirk WA, 2009, EUR J PHYCOL, V44, P231, DOI 10.1080/09670260802573717; Strader LC, 2011, MOL PLANT, V4, P477, DOI 10.1093/mp/ssr006; Sun HG, 2004, PLANT PHYSIOL, V135, P266, DOI 10.1104/pp.103.034900; Swarup R, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00225; Tanaka A, 2017, PROTOPLASMA, V254, P1341, DOI 10.1007/s00709-016-1025-4; Tarakhovskaya ER, 2013, RUSS J PLANT PHYSL+, V60, P176, DOI 10.1134/S1021443713020192; TAYLOR A, 1993, PLANTA, V189, P109; THIMANN KV, 1959, NATURE, V183, P946, DOI 10.1038/183946a0; Tivendale ND, 2014, TRENDS PLANT SCI, V19, P44, DOI 10.1016/j.tplants.2013.09.012; TORREY JG, 1970, AM J BOT, V57, P111, DOI 10.2307/2440384; Waddington C.H., 1940, ORGANISERS GENES; Wang BJ, 2013, PLANT CELL, V25, P202, DOI 10.1105/tpc.112.105999; Wenzel CL, 2007, PLANT J, V49, P387, DOI 10.1111/j.1365-313X.2006.02977.x; West JA, 1999, HYDROBIOLOGIA, V399, P101; WOOD NL, 1979, PLANT SCI LETT, V16, P285, DOI 10.1016/0304-4211(79)90040-3; Xiang DQ, 2011, PLANT PHYSIOL, V156, P346, DOI 10.1104/pp.110.171702; Xu TD, 2014, SCIENCE, V343, P1025, DOI 10.1126/science.1245125; Yue JP, 2014, TRENDS PLANT SCI, V19, P764, DOI 10.1016/j.tplants.2014.07.004; Zhao BL, 2016, MOL PLANT, V9, P582, DOI 10.1016/j.molp.2016.01.007	110	6	6	2	35	AMER SOC PLANT BIOLOGISTS	ROCKVILLE	15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA	0032-0889	1532-2548		PLANT PHYSIOL	Plant Physiol.	JAN	2019	179	1					280	299		10.1104/pp.18.01041			20	Plant Sciences	Plant Sciences	HG4FA	WOS:000454929100023	30420566	Green Published, Bronze			2021-04-07	
J	Vallet, M; Strittmatter, M; Murua, P; Lacoste, S; Dupont, J; Hubas, C; Genta-Jouve, G; Gachon, CMM; Kim, GH; Prado, S				Vallet, Marine; Strittmatter, Martina; Murua, Pedro; Lacoste, Sandrine; Dupont, Joelle; Hubas, Cedric; Genta-Jouve, Gregory; Gachon, Claire M. M.; Kim, Gwang Hoon; Prado, Soizic			Chemically-Mediated Interactions Between Macroalgae, Their Fungal Endophytes, and Protistan Pathogens	FRONTIERS IN MICROBIOLOGY			English	Article						fungal endophytes; macroalgae; protistan pathogens; secondary metabolites; metabolome; molecular interactions; pyrenocines	ECTOCARPUS-SILICULOSUS ECTOCARPALES; MYCOSPHAERELLA SYMBIOSIS; DEFENSE RESPONSES; FITNESS BENEFITS; ALGAL DISEASES; RIBOSOMAL DNA; ASCOPHYLLUM; PHAEOPHYCEAE; ECOLOGY; POLYSIPHONIA	Filamentous fungi asymptomatically colonize the inner tissues of macroalgae, yet their ecological roles remain largely underexplored. Here, we tested if metabolites produced by fungal endophytes might protect their host against a phylogenetically broad spectrum of protistan pathogens. Accordingly, the cultivable fungal endophytes of four brown algal species were isolated and identified based on LSU and SSU sequencing. The fungal metabolomes were tested for their ability to reduce the infection by protistan pathogens in the algal model Ectocarpus siliculosus. The most active metabolomes effective against the oomycetes Eurychasma dicksonii and Anisolpidium ectocarpii, and the phytomixid Maullinia ectocarpii were further characterized chemically. Several pyrenocines isolated from Phaeosphaeria sp. AN596H efficiently inhibited the infection by all abovementioned pathogens. Strikingly, these compounds also inhibited the infection of nori (Pyropia yezoensis) against its two most devastating oomycete pathogens, Olpidiopsis pyropiae, and Pythium porphyrae. We thus demonstrate that fungal endophytes associated with brown algae produce bioactive metabolites which might confer protection against pathogen infection. These results highlight the potential of metabolites to finely-tune the outcome of molecular interactions between algae, their endophytes, and protistan pathogens. This also provide proof-of-concept toward the applicability of such metabolites in marine aquaculture to control otherwise untreatable diseases.	[Vallet, Marine; Genta-Jouve, Gregory; Prado, Soizic] Museum Natl Hist Nat, Unite Mol Commun & Adaptat Microorganismes, UMR 7245, CP 54, Paris, France; [Strittmatter, Martina; Murua, Pedro; Gachon, Claire M. M.] Scottish Marine Inst, Scottish Assoc Marine Sci, Oban, Argyll, Scotland; [Lacoste, Sandrine; Dupont, Joelle] Sorbonne Univ, CNRS, Museum Natl Hist Nat, Inst Systemat Evolut Biodiversite ISYEB,EPHE, Paris, France; [Hubas, Cedric] Univ Antilles, Univ Caen Normandie, Sorbonne Univ,Museum Natl Hist Nat,CNRS, Unite Biol Organisms & Ecosyst Aquat UMR BOREA,IR, Concameau, France; [Hubas, Cedric] Stn Marine Concarneau, Concameau, France; [Genta-Jouve, Gregory] Univ Paris 05, Lab Chim Toxicol Anal & Cellulaire C TAC, UMR CNRS 8638, COMETE, Paris, France; [Kim, Gwang Hoon] Kongju Natl Univ, Dept Biol, Kong Ju, South Korea	Prado, S (corresponding author), Museum Natl Hist Nat, Unite Mol Commun & Adaptat Microorganismes, UMR 7245, CP 54, Paris, France.	sprado@mnhn.fr	Murua, Pedro/J-6397-2014; Murua, Pedro/L-6686-2019	Murua, Pedro/0000-0002-1598-7261; Murua, Pedro/0000-0002-1598-7261; Strittmatter, Martina/0000-0002-1258-9751; Gachon, Claire/0000-0002-3702-7472; Vallet, Marine/0000-0002-6878-0459	ATM Microorganisms grant from the Natural History Museum of Paris; Initiative Structurante Ecosphere continentale et cotiere EC2CO CNRS; Region Ile-de-FranceRegion Ile-de-France; CNRS (France)Centre National de la Recherche Scientifique (CNRS); MNHN (Paris, France); UK NERC IOF Pump-priming + scheme [NE/L013223/1]; European Union's Horizon 2020 research and innovation (ALFF) [642575]; Genomia fund; CONICYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) [72130422]; MASTS Visiting Fellowship Scheme; ASSEMBLE [227799]	This work was supported by ATM Microorganisms grant from the Natural History Museum of Paris (SP), ASSEMBLE grant agreement No. 227799 (SP), and Initiative Structurante Ecosphere continentale et cotiere EC2CO CNRS grant (SP). The 400 MHz and 600 MHz NMR spectrometers used in this study were funded jointly by the Region Ile-de-France, the MNHN (Paris, France), and the CNRS (France). This work was funded through the UK NERC IOF Pump-priming + scheme (NE/L013223/1, CMMG), the European Union's Horizon 2020 research and innovation (ALFF No 642575), the Genomia fund (award HERDIR, MS), the CONICYT (BecasChile No 72130422) (PM), and a MASTS Visiting Fellowship Scheme (SP).	Abdel-Wahab MA, 2010, MYCOL PROG, V9, P537, DOI 10.1007/s11557-010-0661-x; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Amagata T, 1998, J ANTIBIOT, V51, P432, DOI 10.7164/antibiotics.51.432; Arnold AE, 2003, P NATL ACAD SCI USA, V100, P15649, DOI 10.1073/pnas.2533483100; Cass BN, 2016, OECOLOGIA, V180, P169, DOI 10.1007/s00442-015-3436-x; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; Cotton A. D., 1907, T BRIT MYCOLOGICAL S, V3, P92, DOI [10.1016/S0007-1536(07)80023-4, DOI 10.1016/S0007-1536(07)80023-4]; Cruz dela T. E. E., 2006, THESIS; DAYTON PK, 1985, ANNU REV ECOL SYST, V16, P215, DOI 10.1146/annurev.es.16.110185.001243; Debbab A, 2012, FUNGAL DIVERS, V57, P45, DOI 10.1007/s13225-012-0191-8; Deckert Ronald J., 2005, Algae, V20, P363; Egan S, 2013, FEMS MICROBIOL REV, V37, P462, DOI 10.1111/1574-6976.12011; Elsebai MF, 2008, PLANTA MED, V74, P1042; Elsebai MF, 2013, STEROIDS, V78, P880, DOI 10.1016/j.steroids.2013.05.003; Flewelling Andrew J, 2013, Microorganisms, V1, P175; Flewelling AJ, 2013, BOT MAR, V56, P287, DOI 10.1515/bot-2012-0224; FRIES N, 1979, PHYSIOL PLANTARUM, V45, P117, DOI 10.1111/j.1399-3054.1979.tb01674.x; Gachon CMM, 2017, EUR J PHYCOL, V52, P133, DOI 10.1080/09670262.2016.1252857; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; GARBARY DJ, 1989, BOT MAR, V32, P181, DOI 10.1515/botm.1989.32.2.181; Garbary DJ, 1995, BOT MAR, V38, P529, DOI 10.1515/botm.1995.38.1-6.529; GARBARY DJ, 1995, BOT MAR, V38, P221, DOI 10.1515/botm.1995.38.1-6.221; GARBARY DJ, 1991, BOT MAR, V34, P391, DOI 10.1515/botm.1991.34.5.391; GLASS NL, 1995, APPL ENVIRON MICROB, V61, P1323, DOI 10.1128/AEM.61.4.1323-1330.1995; Godinho VM, 2013, ISME J, V7, P1434, DOI 10.1038/ismej.2013.77; Haas D, 2005, NAT REV MICROBIOL, V3, P307, DOI 10.1038/nrmicro1129; Harvey JBJ, 2010, FUNGAL BIOL-UK, V114, P82, DOI 10.1016/j.mycres.2009.10.009; Hiruma K, 2016, CELL, V165, P464, DOI 10.1016/j.cell.2016.02.028; Ji HF, 2009, EMBO REP, V10, P194, DOI 10.1038/embor.2009.12; Jones EBG, 2012, MAR FRESHW BOTANY; Kientz B, 2011, BOT MAR, V54, P457, DOI 10.1515/BOT.2011.053; Kim GH, 2014, ALGAE-SEOUL, V29, P249, DOI 10.4490/algae.2014.29.4.249; Kjer J, 2010, NAT PROTOC, V5, P479, DOI 10.1038/nprot.2009.233; Kohlmeyer J, 2003, BOT MAR, V46, P285, DOI 10.1515/BOT.2003.026; Langenfeld A, 2013, FUNGAL BIOL-UK, V117, P124, DOI 10.1016/j.funbio.2012.12.005; Lopez-Villavicencio M, 2010, FUNGAL GENET BIOL, V47, P693, DOI 10.1016/j.fgb.2010.05.002; Loque CP, 2010, POLAR BIOL, V33, P641, DOI 10.1007/s00300-009-0740-0; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; MICHAELIS KC, 1987, MYCOLOGIA, V79, P514, DOI 10.2307/3807589; Muller DG, 2008, CAH BIOL MAR, V49, P59; Murua P, 2017, PROTIST, V168, P468, DOI 10.1016/j.protis.2017.07.001; Nelson JM, 2018, NEW PHYTOL, V218, P1217, DOI 10.1111/nph.15012; Nilsson RH, 2008, EVOL BIOINFORM, V4, P193; Panzer K, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0134377; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Porras-Alfaro A, 2011, ANNU REV PHYTOPATHOL, V49, P291, DOI 10.1146/annurev-phyto-080508-081831; Posada D, 2001, TRENDS ECOL EVOL, V16, P37, DOI 10.1016/S0169-5347(00)02026-7; Rateb ME, 2011, NAT PROD REP, V28, P290, DOI 10.1039/c0np00061b; Redou V., 2016, MARINE MICROBIOME, P99, DOI [DOI 10.1007/978-3-319-33000-6_4, 10.1007/978-3-319-33000-6, DOI 10.1007/978-3-319-33000-6]; Richards TA, 2012, ANNU REV MAR SCI, V4, P495, DOI 10.1146/annurev-marine-120710-100802; Rukachaisirikul V, 2007, CHEM PHARM BULL, V55, P1383, DOI 10.1248/cpb.55.1383; SATO H, 1981, AGR BIOL CHEM TOKYO, V45, P1675, DOI 10.1080/00021369.1981.10864745; SATO H, 1981, AGR BIOL CHEM TOKYO, V45, P795, DOI 10.1080/00021369.1981.10864607; SATO H, 1979, AGR BIOL CHEM TOKYO, V43, P2409, DOI 10.1080/00021369.1979.10863831; Schulz B, 2005, MYCOL RES, V109, P661, DOI 10.1017/S095375620500273X; Schulz B, 1999, MYCOL RES, V103, P1275, DOI 10.1017/S0953756299008540; Singh RP, 2016, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.01488; Singh RP, 2015, APPL MICROBIOL BIOT, V99, P1571, DOI 10.1007/s00253-014-6334-y; SPARACE SA, 1987, CAN J MICROBIOL, V33, P327, DOI 10.1139/m87-055; Sparrow F. K., 1903, AQUATIC PHYCOMYCETES; Stanley S. J., 1991, AUTECOLOGY ULTRASTRU; Swett CL, 2017, FOREST PATHOL, V47, DOI 10.1111/efp.12298; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tapia JE, 2016, FRONT MICROBIOL, V7, DOI [10.3389/fmicb.2016.00197, 10.3389/fmicb.2016.00107]; Tellenbach C, 2012, FEMS MICROBIOL ECOL, V82, P157, DOI 10.1111/j.1574-6941.2012.01415.x; Terhonen E, 2016, BIOL CONTROL, V99, P53, DOI 10.1016/j.biocontrol.2016.04.006; Thomas F, 2014, NEW PHYTOL, V204, P567, DOI 10.1111/nph.12925; Toledo T. R., 2014, PENICILLIUM PAXILLI, DOI [10.1155/2014/767061, DOI 10.1155/2014/767061]; Van Alstyne KL, 2001, CRC MAR SCI, P301; VILGALYS R, 1994, P NATL ACAD SCI USA, V91, P4599, DOI 10.1073/pnas.91.10.4599; VILGALYS R, 1990, J BACTERIOL, V172, P4238, DOI 10.1128/jb.172.8.4238-4246.1990; White T.J., 1990, PCR PROTOCOLS GUIDE, P315; Wichard T, 2018, J CHEM ECOL, V44, P1008, DOI 10.1007/s10886-018-1004-7; Wichard T, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00072; Zhang T, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0130051; Zhang Y, 2009, MAR DRUGS, V7, P97, DOI 10.3390/md7020097; Zuccaro A, 2003, MYCOL RES, V107, P1451, DOI 10.1017/S0953756203008657; Zuccaro A, 2005, FUNGAL COMMUNITY ITS, P533, DOI DOI 10.1201/9781420027891.CH27; Zuccaro A, 2008, APPL ENVIRON MICROB, V74, P931, DOI 10.1128/AEM.01158-07	80	13	13	1	14	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-302X			FRONT MICROBIOL	Front. Microbiol.	DEC 21	2018	9								3161	10.3389/fmicb.2018.03161			13	Microbiology	Microbiology	HF2MV	WOS:000454071500001	30627120	DOAJ Gold, Green Published			2021-04-07	
J	Vigor, C; Reversat, G; Rocher, A; Oger, C; Galano, JM; Vercauteren, J; Durand, T; Tonon, T; Leblanc, C; Potin, P				Vigor, Claire; Reversat, Guillaume; Rocher, Amandine; Oger, Camille; Galano, Jean-Marie; Vercauteren, Joseph; Durand, Thierry; Tonon, Thierry; Leblanc, Catherine; Potin, Philippe			Isoprostanoids quantitative profiling of marine red and brown macroalgae	FOOD CHEMISTRY			English	Article						Macroalgae; Isoprostanoids; Heavy metal; Copper stress; Oxylipins; Micro-LC-MS/MS	CYCLIC OXYGENATED METABOLITES; OXIDATIVE STRESS BIOMARKERS; POLYUNSATURATED FATTY-ACIDS; ECTOCARPUS-SILICULOSUS; PHYTOPROSTANES; FOOD; B-1; DETOXIFICATION; PHYTOFURANS; DERIVATIVES	With the increasing demand for direct human and animal consumption seaweed farming is rapidly expanding worldwide. Macroalgae have colonized aquatic environments in which they are submitted to frequent changes in biotic and abiotic factors that can trigger oxidative stress (OS). Considering that isoprostanoid derivatives may constitute the most relevant OS biomarkers, we were interested to establish their profile in two red and four brown macroalgae. Seven phytoprostanes, three phytofuranes, and four isoprostanes were quantified through a new micro-LC-MS/MS method. The isoprostanoid contents vary greatly among all the samples, the ent-16(RS)-9-epi-ST-Delta(14)-10-PhytoF and the sum of 5-F-2t-IsoP and 5-epi-5F(2t)-IsoP being the major compounds for most of the macroalgae studied. We further quantified these isoprostanoids in macroalgae submitted to heavy metal (copper) exposure. In most of the cases, their concentrations increased after 24 h of copper stress corroborating the original hypothesis. One exception is the decrease of ent-9-L1 PhytoP content in L. digitata.	[Vigor, Claire; Reversat, Guillaume; Rocher, Amandine; Oger, Camille; Galano, Jean-Marie; Vercauteren, Joseph; Durand, Thierry] UM, CNRS, ENSCM, IBMM,Fac Pharm,UMR 5247, 15 Ave Charles Flahault, F-34060 Montpellier 2, France; [Tonon, Thierry; Leblanc, Catherine; Potin, Philippe] Sorbonne Univ, CNRS, Integrat Biol Marine Models LBI2M, SBR, F-29680 Roscoff, France; [Tonon, Thierry] Univ York, Dept Biol, Ctr Novel Agr Prod, York YO10 5DD, N Yorkshire, England	Vigor, C (corresponding author), UM, CNRS, ENSCM, IBMM,Fac Pharm,UMR 5247, 15 Ave Charles Flahault, F-34060 Montpellier 2, France.	claire.vigor@umontpellier.fr	Vercauteren, Joseph/AAF-7151-2019; Tonon, Thierry/A-3214-2009	Vercauteren, Joseph/0000-0002-0201-1235; Tonon, Thierry/0000-0002-1454-6018; Camille, OGER/0000-0002-5177-5792; VIGOR, Claire/0000-0002-1569-0425	EMBRC France (European Marine Biological Resource Centre); project IDEALG (France) [ANR-10-BTBR-04]; European Joint Programming Initiative "A Healthy Diet for a Healthy Life" [JPI HDHL - ANR-15-HDHL-0003]; Canceropole GSO	This work received financial support from EMBRC France (European Marine Biological Resource Centre) through a call of projects 2015. This project was also partly funded by the project IDEALG (France: ANR-10-BTBR-04), by European Joint Programming Initiative "A Healthy Diet for a Healthy Life" (JPI HDHL - ANR-15-HDHL-0003) and by Canceropole GSO (Emergence 2017). We thank our colleagues from the Marine Service of Roscoff during the material collection and the implementation of the experiments.	Aaronson S, 1986, Food Foodways, V1, P311; Barbosa M, 2015, J AGR FOOD CHEM, V63, P6466, DOI 10.1021/acs.jafc.5b01904; Brodie J, 2017, TRENDS PLANT SCI, V22, P726, DOI 10.1016/j.tplants.2017.05.005; Christeller JT, 2014, PLANT PHYSIOL BIOCH, V83, P117, DOI 10.1016/j.plaphy.2014.07.013; Cock JM, 2011, J EXP BOT, V62, P2425, DOI 10.1093/jxb/err117; Collen J, 2003, ARCH ENVIRON CON TOX, V45, P337, DOI 10.1007/s00244-003-0196-0; Craigie JS, 2011, J APPL PHYCOL, V23, P371, DOI 10.1007/s10811-010-9560-4; Cuyamendous C, 2015, CHEM COMMUN, V51, P15696, DOI 10.1039/c5cc05736a; Dillehay TD, 2008, SCIENCE, V320, P784, DOI 10.1126/science.1156533; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Durand T, 2002, J ORG CHEM, V67, P3615, DOI 10.1021/jo0109624; Durand T, 2001, BIOORG MED CHEM LETT, V11, P2495, DOI 10.1016/S0960-894X(01)00473-5; El Fangour S, 2005, J ORG CHEM, V70, P989, DOI 10.1021/jo048179+; El Fangour S, 2004, J ORG CHEM, V69, P2498, DOI 10.1021/jo035638i; Galano JM, 2017, PROG LIPID RES, V68, P83, DOI 10.1016/j.plipres.2017.09.004; Galano JM, 2015, BBA-MOL CELL BIOL L, V1851, P446, DOI 10.1016/j.bbalip.2014.11.004; Galloway AWE, 2012, J PHYCOL, V48, P956, DOI 10.1111/j.1529-8817.2012.01173.x; Gantar M, 2008, J PHYCOL, V44, P260, DOI 10.1111/j.1529-8817.2008.00469.x; Guy A, 2015, FRONT CHEM, V3, DOI 10.3389/fchem.2015.00041; Guy A, 2014, CHEM-EUR J, V20, P6374, DOI 10.1002/chem.201400380; Holdt SL, 2011, J APPL PHYCOL, V23, P543, DOI 10.1007/s10811-010-9632-5; Hurd CL, 2014, SEAWEED ECOLOGY AND PHYSIOLOGY, 2ND EDITION, P1, DOI 10.1017/CBO9781139192637; Jahn U, 2008, ANGEW CHEM INT EDIT, V47, P5894, DOI 10.1002/anie.200705122; Jimenez-Escrig A, 2012, J APPL PHYCOL, V24, P1123, DOI 10.1007/s10811-011-9742-8; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Kumari P, 2013, WOODHEAD PUBL FOOD S, V256, P87, DOI 10.1533/9780857098689.1.87; Kumari P., 2013, ALGAL LIPIDS FATTY A; Leung KS, 2014, CHEM PHYS LIPIDS, V180, P53, DOI 10.1016/j.chemphyslip.2014.02.004; Loeffler C, 2005, PLANT PHYSIOL, V137, P328, DOI 10.1104/pp.104.051714; Medina S, 2015, FREE RADICAL BIO MED, V79, P154, DOI 10.1016/j.freeradbiomed.2014.11.005; Milne GL, 2013, FREE RADICAL BIO MED, V59, P36, DOI 10.1016/j.freeradbiomed.2012.09.030; Minghetti L, 2014, FREE RADICAL BIO MED, V73, P41, DOI 10.1016/j.freeradbiomed.2014.04.025; Mueller S, 2008, PLANT CELL, V20, P768, DOI 10.1105/tpc.107.054809; Newton L, 1951, SEAWEED UTILIZATION; Noschka E, 2009, VET IMMUNOL IMMUNOP, V129, P200, DOI 10.1016/j.vetimm.2008.11.005; Oger C, 2008, ORG LETT, V10, P5087, DOI 10.1021/ol802104z; Oger C, 2010, CHEM-EUR J, V16, P13976, DOI 10.1002/chem.201002304; Pereira H, 2012, MAR DRUGS, V10, P1920, DOI 10.3390/md10091920; Pinto E, 2003, J PHYCOL, V39, P1008, DOI 10.1111/j.0022-3646.2003.02-193.x; Plaza M, 2008, TRENDS FOOD SCI TECH, V19, P31, DOI 10.1016/j.tifs.2007.07.012; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Roncarati F., 2015, AQUAT TOXICOL, V159, P8; Roy J, 2017, FREE RADICAL BIO MED, V102, P229, DOI 10.1016/j.freeradbiomed.2016.12.005; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Thoma I, 2003, PLANT J, V34, P363, DOI 10.1046/j.1365-313X.2003.01730.x; Tseng CK, 1981, BIOL SEAWEEDS, P680; Turner NJ, 2003, CAN J BOT, V81, P283, DOI [10.1139/b03-029, 10.1139/B03-029]; Wielgosz-Collin G, 2016, SEAWEED IN HEALTH AND DISEASE PREVENTION, P185, DOI 10.1016/B978-0-12-802772-1.00007-5; Yonny ME, 2016, J AGR FOOD CHEM, V64, P8296, DOI 10.1021/acs.jafc.6b03011; Yruela I., 2005, BRAZ J PLANT PHYSIOL, V17, P11	51	10	10	4	58	ELSEVIER SCI LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND	0308-8146	1873-7072		FOOD CHEM	Food Chem.	DEC 1	2018	268						452	462		10.1016/j.foodchem.2018.06.111			11	Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics	Chemistry; Food Science & Technology; Nutrition & Dietetics	GO4LM	WOS:000439980500056	30064783	Green Accepted			2021-04-07	
J	Kupper, FC; Miller, EP; Andrews, SJ; Hughes, C; Carpenter, LJ; Meyer-Klaucke, W; Toyama, C; Muramatsu, Y; Feiters, MC; Carrano, CJ				Kupper, Frithjof C.; Miller, Eric P.; Andrews, Stephen J.; Hughes, Claire; Carpenter, Lucy J.; Meyer-Klaucke, Wolfram; Toyama, Chiaki; Muramatsu, Yasuyuki; Feiters, Martin C.; Carrano, Carl J.			Emission of volatile halogenated compounds, speciation and localization of bromine and iodine in the brown algal genome model Ectocarpus siliculosus	JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY			English	Article						Energy-dispersive X-ray analysis; Halocarbons; Methyl iodide; Phaeophyta; X-ray absorption spectroscopy	METHYL-CHLORIDE TRANSFERASE; KELP LAMINARIA-DIGITATA; OXIDATIVE BURST; EURYCHASMA-DICKSONII; 2 CENTURIES; PHAEOPHYCEAE; BROMOPEROXIDASE; ACCUMULATION; METABOLISM; INFECTION	This study explores key features of bromine and iodine metabolism in the filamentous brown alga and genomics model Ectocarpus siliculosus. Both elements are accumulated in Ectocarpus, albeit at much lower concentration factors (2-3 orders of magnitude for iodine, and < 1 order of magnitude for bromine) than e.g. in the kelp Laminaria digitata. Iodide competitively reduces the accumulation of bromide. Both iodide and bromide are accumulated in the cell wall (apoplast) of Ectocarpus, with minor amounts of bromine also detectable in the cytosol. Ectocarpus emits a range of volatile halogenated compounds, the most prominent of which by far is methyl iodide. Interestingly, biosynthesis of this compound cannot be accounted for by vanadium haloperoxidase since the latter have not been found to catalyze direct halogenation of an unactivated methyl group or hydrocarbon so a methyl halide transferase-type production mechanism is proposed.	[Kupper, Frithjof C.] Univ Aberdeen, Oceanlab, Main St, Newburgh AB41 6AA, Scotland; [Kupper, Frithjof C.] Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland; [Miller, Eric P.; Carrano, Carl J.] San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA; [Andrews, Stephen J.; Carpenter, Lucy J.] Univ York, Dept Chem, Wolfson Atmospher Chem Labs, York YO10 5DD, N Yorkshire, England; [Hughes, Claire] Univ York, Dept Environm, York YO10 5NG, N Yorkshire, England; [Meyer-Klaucke, Wolfram] Univ Paderborn, Dept Chem Inorgan Chem, Fac Sci, Warburger Str 100, D-33098 Paderborn, Germany; [Toyama, Chiaki] Natl Inst Adv Ind Sci & Technol, Geol Survey Japan, Tsukuba, Ibaraki 3058567, Japan; [Muramatsu, Yasuyuki] Gakushuin Univ, Dept Chem, Fac Sci, Toshima Ku, Tokyo 1718588, Japan; [Feiters, Martin C.] Radboud Univ Nijmegen, Inst Mol & Mat, Dept Organ Chem, Heyendaalseweg 135, NL-6525 AJ Nijmegen, Netherlands	Kupper, FC (corresponding author), Univ Aberdeen, Oceanlab, Main St, Newburgh AB41 6AA, Scotland.; Kupper, FC (corresponding author), Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland.	fkuepper@abdn.ac.uk	Meyer-Klaucke, Wolfram/G-1148-2010; Carpenter, Lucy J/E-6742-2013; /D-4640-2012; Toyama, Chiaki/L-9345-2018	Toyama, Chiaki/0000-0003-2822-8376; Carpenter, Lucy/0000-0002-6257-3950	UK Natural Environment Research Council (NERC)UK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/D521522/1, NE/F012705/1, NE/K000454/1, 2025 (WP4.5)]; National Science FoundationNational Science Foundation (NSF) [CHE-1664657]; MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland) - Scottish Funding Council [HR09011]	Funding from the UK Natural Environment Research Council (NERC) through grants NE/D521522/1 (FCK), NE/F012705/1 (FCK), NE/K000454/1 (CH), and the Oceans 2025 (WP4.5) program FCK; the National Science Foundation (CHE-1664657) to CJC; and the MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) is gratefully acknowledged. We are also grateful to Claire M.M. Gachon (Scottish Association for Marine Science) for her assistance with the Ectocarpus/Eurychasma cultures. EPM and CJC are grateful to the Advanced Photon Source at Argonne National Laboratory for facilitating the GSECARS X-ray experiments and Tony Lanzirotti for his assistance on the GSECARS beamline and with subsequent data analysis. Furthermore, the authors are grateful for support from the European Community in the framework of the Access to Research Infrastructure Action of the Improving Human Potential Program to the EMBL Hamburg Outstation. Finally, we would like to thank Yuka Uchida (Department of Chemistry, Gakushuin University, Tokyo) for her help with the ICP-MS analyses mentioned here.	Andrews SJ, 2015, OCEAN SCI, V11, P313, DOI 10.5194/os-11-313-2015; Ar Gall E, 2004, BOT MAR, V47, P30; Balard AJ, 1826, ANN CHIM PHYS, V32, P337; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; BEISSNER RS, 1981, BIOCHEMISTRY-US, V20, P3724, DOI 10.1021/bi00516a009; BHATTACHARYA D, 1991, J MOL EVOL, V33, P525, DOI 10.1007/BF02102805; Bottger LH, 2012, J EXP BOT, V63, P5763, DOI 10.1093/jxb/ers225; Carpenter LJ, 2000, GLOBAL BIOGEOCHEM CY, V14, P1191, DOI 10.1029/2000GB001257; Chai JY, 2007, GEOSTAND GEOANAL RES, V31, P143, DOI 10.1111/j.1751-908X.2007.00856.x; Chance R, 2009, ESTUAR COAST SHELF S, V82, P406, DOI 10.1016/j.ecss.2009.02.004; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2012, ADV BOT RES, V64, P141, DOI 10.1016/B978-0-12-391499-6.00005-0; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Colin C, 2005, J BIOL INORG CHEM, V10, P156, DOI 10.1007/s00775-005-0626-8; Colin C, 2003, J BIOL CHEM, V278, P23545, DOI 10.1074/jbc.M300247200; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; Feiters MC, 2005, J SYNCHROTRON RADIAT, V12, P85, DOI 10.1107/S0909049504027815; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Gay-Lussac L-J, 1813, ANN CHIM, V88, P311; Gonzales J, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0180755; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Kupper FC, 2015, IODINE CHEMISTRY AND APPLICATIONS, P557; Kupper FC, 2014, J PHYCOL, V50, P652, DOI 10.1111/jpy.12199; Kupper FC, 2013, J EXP BOT, V64, P2653, DOI 10.1093/jxb/ert110; Kupper FC, 2011, ANGEW CHEM INT EDIT, V50, P11598, DOI 10.1002/anie.201100028; Kupper FC, 2009, PLANT CELL PHYSIOL, V50, P789, DOI 10.1093/pcp/pcp023; Kupper FC, 1998, PLANTA, V207, P163, DOI 10.1007/s004250050469; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; La Barre S, 2010, MAR DRUGS, V8, P988, DOI 10.3390/md8040988; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; McFiggans G, 2004, ATMOS CHEM PHYS, V4, P701, DOI 10.5194/acp-4-701-2004; Miller EP, 2014, J EXP BOT, V65, P585, DOI 10.1093/jxb/ert406; Muller DG, 2008, CAH BIOL MAR, V49, P59; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Newville M, 2013, J PHYS CONF SER, V430, DOI 10.1088/1742-6596/430/1/012007; Ni XH, 1999, P NATL ACAD SCI USA, V96, P3611, DOI 10.1073/pnas.96.7.3611; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Potin P, 2006, BIOLOGICAL ADHESIVES, P105, DOI 10.1007/978-3-540-31049-5_6; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; RUSSELL G, 1983, MAR ECOL PROG SER, V13, P303, DOI 10.3354/meps013303; RUSSELL G, 1983, MAR ECOL PROG SER, V11, P181, DOI 10.3354/meps011181; SAENKO GN, 1978, MAR BIOL, V47, P243, DOI 10.1007/BF00541002; Schnetger B, 1996, ANALYST, V121, P1627, DOI 10.1039/an9962101627; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Starr R.C., 1987, Journal of Phycology, V23, P1; Strittmatter M, 2016, PLANT CELL ENVIRON, V39, P259, DOI 10.1111/pce.12533; THEILER R, 1978, SCIENCE, V202, P1094, DOI 10.1126/science.202.4372.1094; Thomas F, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0021475; Tymon TM, 2017, J INORG BIOCHEM, V177, P82, DOI 10.1016/j.jinorgbio.2017.09.003; Verhaeghe EF, 2008, J BIOL INORG CHEM, V13, P257, DOI 10.1007/s00775-007-0319-6; Vilter H, 1995, VANADIUM ITS ROLE LI, P325; Wojtaszek P, 1997, BIOCHEM J, V322, P681, DOI 10.1042/bj3220681; WUOSMAA AM, 1990, SCIENCE, V249, P160, DOI 10.1126/science.2371563; Zambounis A, 2012, ALGAE-SEOUL, V27, P21, DOI 10.4490/algae.2012.27.1.021	59	10	10	1	13	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0949-8257	1432-1327		J BIOL INORG CHEM	J. Biol. Inorg. Chem.	OCT	2018	23	7					1119	1128		10.1007/s00775-018-1539-7			10	Biochemistry & Molecular Biology; Chemistry, Inorganic & Nuclear	Biochemistry & Molecular Biology; Chemistry	GY5BG	WOS:000448583900016	29523971	Green Published, Other Gold, Green Accepted			2021-04-07	
J	Hanyuda, T; Hansen, GI; Kawai, H				Hanyuda, Takeaki; Hansen, Gayle I.; Kawai, Hiroshi			Genetic identification of macroalgal species on Japanese tsunami marine debris and genetic comparisons with their wild populations	MARINE POLLUTION BULLETIN			English	Article						Genetic marker; Great East Japan Earthquake; Invasion; Macroalgae; Tsunami debris	CUTLERIA-CYLINDRICA CUTLERIALES; INTRODUCED POPULATIONS; UNDARIA-PINNATIFIDA; PHAEOPHYCEAE; CHLOROPHYTA; RHODOPHYTA; SUCCESSION; DIVERSITY; PACIFIC; PROGRAM	Since 2012 a huge amount of marine debris caused by the 2011 Great East Japan Earthquake and Tsunami has been arriving on Northeastern Pacific shores. Often healthy macroalgae were attached to them, which may become introduced to the Northwestern Pacific coasts and disturb their ecosystems. In order to elucidate the diversity of those macroalgae, and to establish a basis for detecting their new introduction to Northwestern Pacific coasts, we have examined their species diversity by morphology and genetic identifications. We have obtained gene sequences for 205 specimens, and identified 49 species as Japanese Tsunami Marine Debris (JTMD) macroalgae. Most of them are known to be distributed in Japan and showed identical or very closely related genetic types to those of Tohoku, and confirmed to be originated from the area. Several species such as Ceramium sungminbooi, Ectocarpus crouaniorum, Polysiphonia koreana, etc. have not been reported from Japan, but this is explained by the shortage of taxonomic information.	[Hanyuda, Takeaki; Kawai, Hiroshi] Kobe Univ, Res Ctr Inland Seas, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan; [Hansen, Gayle I.] Oregon State Univ, HMSC EPA, 2111 SE Marine Sci Dr, Newport, OR 97365 USA	Kawai, H (corresponding author), Kobe Univ, Res Ctr Inland Seas, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan.	kawai@kobe-u.ac.jp			Japanese Ministry of the Environment through the North Pacific Marine Science Organization (PICES); JSPSMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [26440217]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [16H04832] Funding Source: KAKEN	We are grateful to Dr Eric Henry for his valuable comments. This work was supported by the Japanese Ministry of the Environment through the North Pacific Marine Science Organization (PICES) and the JSPS Grants-in-Aid for Scientific Research (No. 26440217) to T.H. The US Environmental Protection Agency provided laboratory space for the Oregon part of this study.	Boudouresque CF, 2002, MAR POLLUT BULL, V44, P32, DOI 10.1016/S0025-326X(01)00150-3; Bustamante DE, 2015, EUR J PHYCOL, V50, P330, DOI 10.1080/09670262.2015.1060633; Clement M, 2000, MOL ECOL, V9, P1657, DOI 10.1046/j.1365-294x.2000.01020.x; Curiel D, 1998, HYDROBIOLOGIA, V385, P17, DOI 10.1023/A:1003437105147; DIECK IT, 1992, PHYCOLOGIA, V31, P147, DOI 10.2216/i0031-8884-31-2-147.1; Funano T., 1978, HOKUSUISHI GEPPO, V35, P23; FUNANO T, 1980, HOKUSUISHI GEPPO, V37, P181; Hansen I.G., MAR POLLUT B; Hanyuda T, 2016, PHYCOL RES, V64, P102, DOI 10.1111/pre.12123; Katoh K, 2008, BRIEF BIOINFORM, V9, P286, DOI 10.1093/bib/bbn013; Kawai H, 2012, PHYCOL RES, V60, P241, DOI 10.1111/j.1440-1835.2012.00651.x; Kirkendale L, 2013, J PHYCOL, V49, P69, DOI 10.1111/jpy.12016; KITAYAMA T, 1992, PHYCOLOGIA, V31, P449, DOI 10.2216/i0031-8884-31-5-449.1; Kogishi K, 2010, J PHYCOL, V46, P553, DOI 10.1111/j.1529-8817.2010.00818.x; Lis J T, 1980, Methods Enzymol, V65, P347; Ministry of the Environment (MOE), 2012, EST TOT AM DEBR WASH; Ogawa T, 2013, PHYCOLOGIA, V52, P637, DOI 10.2216/13-199.1; Silvestro D, 2012, ORG DIVERS EVOL, V12, P335, DOI 10.1007/s13127-011-0056-0; Smith Jennifer E., 2002, Pacific Science, V56, P299, DOI 10.1353/psc.2002.0030; Streftaris N, 2005, OCEANOGR MAR BIOL, V43, P419; Uwai SY, 2006, PHYCOLOGIA, V45, P687, DOI 10.2216/05-66.1; West JA, 2016, ALGAE-SEOUL, V31, P289, DOI 10.4490/algae.2016.31.10.20; YOSHIDA T, 2015, JAPANESE J PHYCOLOGY, V63, P129	23	14	14	0	8	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND	0025-326X	1879-3363		MAR POLLUT BULL	Mar. Pollut. Bull.	JUL	2018	132		C		SI		74	81		10.1016/j.marpolbul.2017.06.053			8	Environmental Sciences; Marine & Freshwater Biology	Environmental Sciences & Ecology; Marine & Freshwater Biology	GO6AS	WOS:000440117100009	28684109	Green Published, Other Gold			2021-04-07	
J	Avia, K; Lipinska, AP; Mignerot, L; Montecinos, AE; Jamy, M; Ahmed, S; Valero, M; Peters, AE; Cock, JM; Roze, D; Coelho, SM				Avia, Komlan; Lipinska, Agnieszka P.; Mignerot, Laure; Montecinos, Alejandro E.; Jamy, Mahwash; Ahmed, Sophia; Valero, Myriam; Peters, Akira E.; Cock, J. Mark; Roze, Denis; Coelho, Susana M.			Genetic Diversity in the UV Sex Chromosomes of the Brown Alga Ectocarpus	GENES			English	Article						UV sex chromosomes; pseudoautosomal region; brown algae; neutral diversity	HILL-ROBERTSON INTERFERENCE; X-LINKED LOCI; SSP MAYS L.; PSEUDOAUTOSOMAL REGION; Y-CHROMOSOME; NATURAL-POPULATIONS; RECOMBINATION RATES; DNA POLYMORPHISM; DROSOPHILA-MELANOGASTER; EVOLUTIONARY DYNAMICS	Three types of sex chromosome system exist in nature: diploid XY and ZW systems and haploid UV systems. For many years, research has focused exclusively on XY and ZW systems, leaving UV chromosomes and haploid sex determination largely neglected. Here, we perform a detailed analysis of DNA sequence neutral diversity levels across the U and V sex chromosomes of the model brown alga Ectocarpus using a large population dataset. We show that the U and V non-recombining regions of the sex chromosomes (SDR) exhibit about half as much neutral diversity as the autosomes. This difference is consistent with the reduced effective population size of these regions compared with the rest of the genome, suggesting that the influence of additional factors such as background selection or selective sweeps is minimal. The pseudoautosomal region (PAR) of this UV system, in contrast, exhibited surprisingly high neutral diversity and there were several indications that genes in this region may be under balancing selection. The PAR of Ectocarpus is known to exhibit unusual genomic features and our results lay the foundation for further work aimed at understanding whether, and to what extent, these structural features underlie the high level of genetic diversity. Overall, this study fills a gap between available information on genetic diversity in XY/ZW systems and UV systems and significantly contributes to advancing our knowledge of the evolution of UV sex chromosomes.	[Avia, Komlan; Lipinska, Agnieszka P.; Mignerot, Laure; Jamy, Mahwash; Ahmed, Sophia; Cock, J. Mark; Coelho, Susana M.] UPMC Univ Paris 06, Stn Biol Roscoff, Integrat Biol Marine Models, Algal Genet Grp,Sorbonne Univ,CNRS, CS 90074, F-29688 Roscoff, France; [Avia, Komlan; Montecinos, Alejandro E.; Valero, Myriam; Roze, Denis] Univ Paris VI, Sorbonne Univ, Evolutionary Biol & Ecol Algae, CNRS,UC,UNCAH,UMI 3614,UPMC, F-29688 Roscoff, France; [Montecinos, Alejandro E.] Univ Austral Chile, Fac Ciencias, Inst Ciencias Ambientales & Evolut, Casilla 567, Valdivia, Chile; [Peters, Akira E.] Bezhin Rosko, F-29250 Santec, France	Avia, K (corresponding author), UPMC Univ Paris 06, Stn Biol Roscoff, Integrat Biol Marine Models, Algal Genet Grp,Sorbonne Univ,CNRS, CS 90074, F-29688 Roscoff, France.; Avia, K (corresponding author), Univ Paris VI, Sorbonne Univ, Evolutionary Biol & Ecol Algae, CNRS,UC,UNCAH,UMI 3614,UPMC, F-29688 Roscoff, France.	komlan.avia@sb-roscoff.fr; alipinska@sb-roscoff.fr; laure.mignerot@sb-roscoff.fr; jano.montecinos@gmail.com; mahwash.jamy@ebc.uu.se; S.M.Ahmed@leeds.ac.uk; myriam.valero@sb-roscoff.fr; akirapeters@gmail.com; cock@sb-roscoff.fr; denis.roze@sb-roscoff.fr; coelho@sb-roscoff.fr	Avia, Komlan/E-6850-2015; Valero, Myriam/M-6052-2019; Coelho, Susana/ABH-8166-2020	Avia, Komlan/0000-0001-6212-6774; Valero, Myriam/0000-0002-9000-1423; Cock, J. Mark/0000-0002-2650-0383	CNRSCentre National de la Recherche Scientifique (CNRS)European Commission; Sorbonne Universite; project IDEALG (France) [ANR-10-BTBR-04]; Brittany RegionRegion Bretagne [SAD 2015-ECTOQTL 9119]; Becas-Chile doctoral grant (CONICYT, advanced human resources program); ERCEuropean Research Council (ERC)European Commission [638240]; EU FP7 "capacities" specific program ASSEMBLE	This work was supported by the CNRS, Sorbonne Universite, the project IDEALG (France: ANR-10-BTBR-04), the Brittany Region grant SAD 2015-ECTOQTL 9119 to K.A., the Becas-Chile doctoral grant to A.E.M. (CONICYT, advanced human resources program) and the ERC (grant agreement 638240). Field collections in Chile and Italy (2012/2013) were supported by two grants from the EU FP7 "capacities" specific program ASSEMBLE to A.F.P. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.	AGUADE M, 1989, GENETICS, V122, P607; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Avia K, 2017, SCI REP-UK, V7, DOI 10.1038/srep43241; Bachtrog D, 2013, NAT REV GENET, V14, P113, DOI 10.1038/nrg3366; Bachtrog D, 2011, TRENDS GENET, V27, P350, DOI 10.1016/j.tig.2011.05.005; Badouin H, 2017, MOL ECOL, V26, P2041, DOI 10.1111/mec.13976; BEGUN DJ, 1992, NATURE, V356, P519, DOI 10.1038/356519a0; Broman K.W., XOI TOOLS ANAL CROSS; Broman KW, 2003, BIOINFORMATICS, V19, P889, DOI 10.1093/bioinformatics/btg112; BULL JJ, 1978, AM NAT, V112, P245, DOI 10.1086/283267; Catchen J, 2013, MOL ECOL, V22, P3124, DOI 10.1111/mec.12354; Catchen JM, 2017, MOL ECOL RESOUR, V17, P362, DOI 10.1111/1755-0998.12669; Charlesworth B, 2000, PHILOS T R SOC B, V355, P1563, DOI 10.1098/rstb.2000.0717; Charlesworth B, 2014, ANNU REV GENET, V48, P383, DOI 10.1146/annurev-genet-120213-092525; Charlesworth B, 2014, EVOLUTION, V68, P1339, DOI 10.1111/evo.12364; Charlesworth B, 2012, GENETICS, V190, P5, DOI 10.1534/genetics.111.134288; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P262, DOI 10.1101/pdb.prot067942; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Comeron JM, 2008, HEREDITY, V100, P19, DOI 10.1038/sj.hdy.6801059; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; Cutter AD, 2013, NAT REV GENET, V14, P262, DOI 10.1038/nrg3425; Danecek P, 2011, BIOINFORMATICS, V27, P2156, DOI 10.1093/bioinformatics/btr330; Dvorak J, 1998, GENETICS, V148, P423; ELLIS N, 1990, NATURE, V344, P663, DOI 10.1038/344663a0; Guirao-Rico S, 2017, MOL ECOL, V26, P1357, DOI 10.1111/mec.13969; Handley LJL, 2006, HEREDITY, V96, P298, DOI 10.1038/sj.hdy.6800803; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hellborg L, 2004, MOL BIOL EVOL, V21, P158, DOI 10.1093/molbev/msh008; Hellmann I, 2003, AM J HUM GENET, V72, P1527, DOI 10.1086/375657; HILL WG, 1966, GENET RES, V8, P269, DOI 10.1017/S0016672300010156; Hough J, 2017, GENETICS, V207, P685, DOI 10.1534/genetics.117.300142; HUDSON RR, 1988, GENETICS, V120, P831; Jordan CY, 2012, EVOLUTION, V66, P505, DOI 10.1111/j.1558-5646.2011.01448.x; Kawakami T, 2014, MOL ECOL, V23, P4035, DOI 10.1111/mec.12810; KIMURA M, 1969, GENETICS, V61, P893; Kirkpatrick M, 2014, GENETICS, V197, P531, DOI 10.1534/genetics.113.156026; Kirkpatrick M, 2010, GENETICS, V184, P1141, DOI 10.1534/genetics.109.113555; Kraft T, 1998, GENETICS, V150, P1239; Lander ES, 2001, NATURE, V409, P860, DOI 10.1038/35057062; LI H, 2013, 13033997 ARXIV, V1303, P3997, DOI DOI 10.1093/BI0INF0RMATICS/BTP352; Librado P, 2009, BIOINFORMATICS, V25, P1451, DOI 10.1093/bioinformatics/btp187; Lindblad-Toh K, 2005, NATURE, V438, P803, DOI 10.1038/nature04338; Lipinska A.P., 2018, BIORXIV, DOI [10.1101/290809, DOI 10.1101/290809]; Lipinska AP, 2015, EUR J PHYCOL, V50, P68; Lipinska AP, 2017, GENOME BIOL, V18, DOI 10.1186/s13059-017-1201-7; Lipinska AP, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140535; Lowry DB, 2017, MOL ECOL RESOUR, V17, P142, DOI 10.1111/1755-0998.12635; Luthringer R, 2015, MOL BIOL EVOL, V32, P2973, DOI 10.1093/molbev/msv173; Makova KD, 2002, NATURE, V416, P624, DOI 10.1038/416624a; Martin SH, 2016, GENETICS, V203, P525, DOI 10.1534/genetics.115.183285; McAllister BF, 1999, GENETICS, V153, P221; McVean GAT, 2000, GENETICS, V155, P929; Mignerot L., 2018, GENETIC CELLUL UNPUB; Montecinos AE, 2017, MOL ECOL, V26, P3497, DOI 10.1111/mec.14098; Montecinos AE, 2017, J PHYCOL, V53, P17, DOI 10.1111/jpy.12452; Nachman MW, 1997, GENETICS, V147, P1303; Nachman MW, 1998, GENETICS, V150, P1133; Otto SP, 2011, TRENDS GENET, V27, P358, DOI 10.1016/j.tig.2011.05.001; Pearson G, 2006, EUR J PHYCOL, V41, P97, DOI 10.1080/09670260500505011; Peterson BK, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0037135; Pueschel C.M., 1990, BIOL RED ALGAE; Qiu S, 2013, GENETICS, V194, P663, DOI 10.1534/genetics.113.152397; Qiu S, 2010, P ROY SOC B-BIOL SCI, V277, P3283, DOI 10.1098/rspb.2010.0606; Sayres MAW, 2018, GENOME BIOL EVOL, V10, P1064, DOI 10.1093/gbe/evy039; Sayres MAW, 2014, PLOS GENET, V10, DOI 10.1371/journal.pgen.1004064; Singh ND, 2014, MOL BIOL EVOL, V31, P2612, DOI 10.1093/molbev/msu203; Smeds L, 2016, GENOME RES, V26, P1211, DOI 10.1101/gr.204669.116; Smeds L, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms8330; Smeds L, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms6448; STEPHAN W, 1989, GENETICS, V121, P89; Stephan W, 1998, GENETICS, V150, P1585; Stoletzki N, 2011, MOL BIOL EVOL, V28, P63, DOI 10.1093/molbev/msq249; TAJIMA F, 1989, GENETICS, V123, P585; Tenaillon MI, 2004, MOL BIOL EVOL, V21, P1214, DOI 10.1093/molbev/msh102; Tenaillon MI, 2002, GENETICS, V162, P1401; Tenaillon MI, 2001, P NATL ACAD SCI USA, V98, P9161, DOI 10.1073/pnas.151244298; Toder R, 1998, MAMM GENOME, V9, P373, DOI 10.1007/s003359900772; VANOOIJEN JW, 2004, SOFTWARE MAPPING QUA; Wickham H, 2011, WIRES COMPUT STAT, V3, P180, DOI 10.1002/wics.147; Willing EM, 2011, BIOINFORMATICS, V27, P2187, DOI 10.1093/bioinformatics/btr346	82	6	6	0	9	MDPI	BASEL	ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND	2073-4425			GENES-BASEL	Genes	JUN	2018	9	6							286	10.3390/genes9060286			18	Genetics & Heredity	Genetics & Heredity	GK8RG	WOS:000436494200018	29882839	DOAJ Gold, Green Published			2021-04-07	
J	Mikami, K; Ito, M; Taya, K; Kishimoto, I; Kobayashi, T; Itabashi, Y; Tanaka, R				Mikami, Koji; Ito, Meiko; Taya, Kensuke; Kishimoto, Ikuya; Kobayashi, Takuya; Itabashi, Yutaka; Tanaka, Ryusuke			Parthenosporophytes of the brown alga Ectocarpus siliculosus exhibit sexdependent differences in thermotolerance as well as fatty acid and sterol composition	MARINE ENVIRONMENTAL RESEARCH			English	Article						Ectocarpus siculosus; Parthenosporophyte; Sex; Heat stress; Thermotolerance; Fatty acid; Sterol	HEAT-SHOCK RESPONSE; MEMBRANE-FLUIDITY; PHOTOSYNTHETIC PIGMENTS; HIGH-TEMPERATURE; PLANT STEROLS; STRESS; LIPIDS; PERCEPTION; MECHANISMS; EXPRESSION	In the filamentous brown alga Ectocarpus siliculosus, male and female sex is expressed during the haploid parthenosporophyte phase of the life cycle. Here, we found that male parthenosporophytes displayed thermotolerance whereas female specimens displayed severely reduced viability at 25 degrees C and 28 degrees C. Profiling of polyunsaturated fatty acids showed that n-3 and n-6 were the predominant species in male and female parthenosporophytes, respectively, and that the n-3/n-6 fatty acid ratio was not affected by a temperature change. Both male and female parthenosporophytes contained the sterols fucosterol, cholesterol, and ergosterol, but these were present at higher levels at 10-25 degrees C in female specimens than in males. Thus, these fatty acids and sterols would be expected to make the membranes more rigid in the female compared to the male, which is opposite to the paradigm that increased rigidity confers thermotolerance. Our results suggest that the sex-dependent thermotolerance in E. siliculosus parthenosporophytes is not explained by the relationship between membrane fluidity and differences in fatty acids and sterol compositions.	[Mikami, Koji; Itabashi, Yutaka] Hokkaido Univ, Fac Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, Japan; [Mikami, Koji] Shanghai Ocean Univ, Coll Fisheries & Life Sci, 999 Huchenghuan Rd, Shanghai 201306, Peoples R China; [Ito, Meiko; Tanaka, Ryusuke] Miyazaki Univ, Fac Agr, Dept Marine Biol & Environm Sci, Gakuen Kibanadai Nishi 1-1, Miyazaki 8892192, Japan; [Taya, Kensuke; Kishimoto, Ikuya; Kobayashi, Takuya] Hokkaido Univ, Grad Sch Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, Japan	Mikami, K (corresponding author), Hokkaido Univ, Fac Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, Japan.	komikami@fish.hokudai.ac.jp	Tanaka, Ryusuke/F-8798-2017; Tanaka, Ryusuke/AAE-4191-2020; Mikami, Koji/A-9825-2012	Tanaka, Ryusuke/0000-0003-4044-3474; Tanaka, Ryusuke/0000-0003-4044-3474; Mikami, Koji/0000-0003-3811-0072	KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [15H04539]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [15H04539] Funding Source: KAKEN	We thank Keiji Sasuga of the Suzuyo Research Institute, Shimizu, Japan, for kindly providing statistical analysis. This work was also supported in part by KAKENHI Grants 15H04539.	AKNIN M, 1990, COMP BIOCHEM PHYS B, V96, P559, DOI 10.1016/0305-0491(90)90056-Y; Bogaert Kenny A, 2013, Methods Mol Biol, V959, P97, DOI 10.1007/978-1-62703-221-6_6; Carratu L, 1996, P NATL ACAD SCI USA, V93, P3870, DOI 10.1073/pnas.93.9.3870; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; de Mendoza D, 2014, ANNU REV MICROBIOL, V68, P101, DOI 10.1146/annurev-micro-091313-103612; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; FLEURY BG, 1994, PHYTOCHEMISTRY, V37, P1447, DOI 10.1016/S0031-9422(00)90430-8; FORD RC, 1983, PLANTA, V158, P35, DOI 10.1007/BF00395400; Gerasimenko N, 2016, OPEN J MAR SCI, V6, P498, DOI DOI 10.4236/ojms.2016.64041; Gerasimenko NI, 2014, RUSS J PLANT PHYSL+, V61, P893, DOI 10.1134/S1021443714050082; Gerasimenko NI, 2011, RUSS J PLANT PHYSL+, V58, P885, DOI 10.1134/S1021443711050086; Gihan A, 2014, INT J PHARM PHYTOCHE, V6, P894; Hartmann MA, 1998, TRENDS PLANT SCI, V3, P170, DOI 10.1016/S1360-1385(98)01233-3; Horvath I, 1998, P NATL ACAD SCI USA, V95, P3513, DOI 10.1073/pnas.95.7.3513; Ito M, 2017, FOOD ANAL METHOD, V10, P2692, DOI 10.1007/s12161-017-0841-2; Konigshofer H, 2008, PLANT CELL ENVIRON, V31, P1771, DOI 10.1111/j.1365-3040.2008.01880.x; Kumari P, 2013, WOODHEAD PUBL FOOD S, V256, P87, DOI 10.1533/9780857098689.1.87; LANDE MB, 1995, J GEN PHYSIOL, V106, P67, DOI 10.1085/jgp.106.1.67; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; Leach MD, 2014, EUKARYOT CELL, V13, P1077, DOI 10.1128/EC.00138-14; Luneva O G, 2007, Pathophysiology, V14, P41, DOI 10.1016/j.pathophys.2006.12.001; Marinho GS, 2015, MAR DRUGS, V13, P4357, DOI 10.3390/md13074357; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Mikami K, 2003, PROG LIPID RES, V42, P527, DOI 10.1016/S0163-7827(03)00036-5; Mikami K, 2009, MAR BIOTECHNOL, V11, P563, DOI 10.1007/s10126-008-9172-z; Mora MP, 1999, CHEM PHYS LIPIDS, V101, P255, DOI 10.1016/S0009-3084(99)00067-5; Murakami Y, 2000, SCIENCE, V287, P476, DOI 10.1126/science.287.5452.476; Orvar BL, 2000, PLANT J, V23, P785, DOI 10.1046/j.1365-313x.2000.00845.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Ruelland E, 2010, ENVIRON EXP BOT, V69, P225, DOI 10.1016/j.envexpbot.2010.05.011; Sanchez-Machado DI, 2004, BIOMED CHROMATOGR, V18, P183, DOI 10.1002/bmc.316; Sangwan V, 2002, PLANT J, V31, P629, DOI 10.1046/j.1365-313X.2002.01384.x; Tanaka R, 2016, FOOD CHEM, V212, P104, DOI 10.1016/j.foodchem.2016.05.166; Tillman TS, 2003, CELL BIOCHEM BIOPHYS, V38, P161, DOI 10.1385/CBB:38:2:161; Torok Z, 2014, BBA-BIOMEMBRANES, V1838, P1594, DOI 10.1016/j.bbamem.2013.12.015; Upchurch RG, 2008, BIOTECHNOL LETT, V30, P967, DOI 10.1007/s10529-008-9639-z; Vigh L, 1998, TRENDS BIOCHEM SCI, V23, P369, DOI 10.1016/S0968-0004(98)01279-1; VIGH L, 1993, P NATL ACAD SCI USA, V90, P9090, DOI 10.1073/pnas.90.19.9090; Weijers RNM, 2012, CURR DIABETES REV, V8, P390, DOI 10.2174/157339912802083531	46	4	4	1	14	ELSEVIER SCI LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND	0141-1136	1879-0291		MAR ENVIRON RES	Mar. Environ. Res.	JUN	2018	137						188	195		10.1016/j.marenvres.2018.02.003			8	Environmental Sciences; Marine & Freshwater Biology; Toxicology	Environmental Sciences & Ecology; Marine & Freshwater Biology; Toxicology	GG5TI	WOS:000432758200019	29459067	Green Published			2021-04-07	
J	Gonzalez, A; Saez, CA; Morales, B; Moenne, A				Gonzalez, Alberto; Saez, Claudio A.; Morales, Bernardo; Moenne, Alejandra			Copper-induced activation of TRP channels promotes extracellular calcium entry and activation of CaMK, PKA, PKC, PKG and CBLPK leading to increased expression of antioxidant enzymes in Ectocarpus siliculosus	PLANT PHYSIOLOGY AND BIOCHEMISTRY			English	Article						Copper; Calcium; Ectocarpus siliculosus; Marine macroalga; TRP channels	DIFFERENT POLLUTION HISTORIES; DEPENDENT PROTEIN-KINASES; ULVA-COMPRESSA; ABIOTIC STRESS; EVOLUTION; RESPONSES; POTENT; RED; CHLAMYDOMONAS; CHLOROPHYTA	The existence of functional Transient Receptor Potential (TRP) channels was analyzed in Ectocarpus siliculosus using agonists of human TRPs and specific antagonists of TRPA1, TRPC5, TRPM8 and TRPV; intracellular calcium was detected for 60 min. Increases in intracellular calcium were observed at 13, 29, 39 and 50-52 min, which appeared to be mediated by the activation of TRPM8/V1 at 13 min, TRPV1 at 29 min, TRPA1/V1 at 39 min and TRPA1/C5 at 50-52 min. In addition, intracellular calcium increases appear to be due to extra cellular calcium entry, not requiring protein kinase activation. On the other hand, 2.5 mu M copper exposure induced increased intracellular calcium at 13, 29, 39 and 51 min, likely due to the activation of a TRPA1/V1 at 13 min, TRPA1/C5/M8 at 29 min, TRPC5/M8 at 39 min, and a TRPC5/V1 at 51 min. The increases in intracellular calcium induced by copper were due to extracellular calcium entry and required protein kinase activation. Furthermore, from 3 to 24 h, copper exposure induced an increase in the level of transcripts encoding antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, glutathione reductase and peroxiredoxin. The described upregulation decreased with inhibitors of CaMK, PKA, PKC, PKG and CBLPK, as well as with a mixture of TRP inhibitors. Thus, copper induces the activation of TRP channels allowing extracellular calcium entry as well as the activation of CaMK, PKA, PKC, PKG and CBLPK leading to increased expression of genes encoding antioxidant enzymes in E. siliculosus.	[Gonzalez, Alberto; Morales, Bernardo; Moenne, Alejandra] Univ Santiago Chile, Fac Chem & Biol, Santiago, Chile; [Saez, Claudio A.] Univ Playa Ancha, Ctr Adv Studies, Lab Coastal Environm Res, Vina Del Mar, Chile	Gonzalez, A; Moenne, A (corresponding author), Univ Santiago Chile, Fac Chem & Biol, Santiago, Chile.	alberto.gonzalezfi@usach.cl; alejandra.moenne@usach.cl	Saez, Claudio/F-5978-2015; Figueroa, Alberto Gonzalez/AAA-7911-2020	Saez, Claudio/0000-0002-5037-3484; Figueroa, Alberto Gonzalez/0000-0002-5777-9022; Moenne, Alejandra/0000-0002-7309-9713	project FondecytComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [3150440]	This work was financed by postdoctoral project Fondecyt 3150440 granted to A.G.	Almeida MC, 2012, J NEUROSCI, V32, P2086, DOI 10.1523/JNEUROSCI.5606-11.2012; Arias-Darraz L, 2015, PLANT CELL, V27, P177, DOI 10.1105/tpc.114.131862; Baurain D, 2010, MOL BIOL EVOL, V27, P1698, DOI 10.1093/molbev/msq059; Butt E, 1995, BRIT J PHARMACOL, V116, P3110, DOI 10.1111/j.1476-5381.1995.tb15112.x; Chan CX, 2011, PLANT PHYSIOL, V155, P1552, DOI 10.1104/pp.111.173500; Chan CX, 2011, CURR BIOL, V21, P328, DOI 10.1016/j.cub.2011.01.037; Choudhury FK, 2017, PLANT J, V90, P856, DOI 10.1111/tpj.13299; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; Eid SR, 2008, MOL PAIN, V4, DOI 10.1186/1744-8069-4-48; Fujiu K, 2011, NAT CELL BIOL, V13, P630, DOI 10.1038/ncb2214; Gaudet R, 2008, MOL BIOSYST, V4, P372, DOI 10.1039/b801481g; Gill SS, 2010, PLANT PHYSIOL BIOCH, V48, P909, DOI 10.1016/j.plaphy.2010.08.016; Gomez M, 2017, ALGAL RES, V26, P115, DOI 10.1016/j.algal.2017.07.009; Gomez M, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.00754; Gomez M, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00182; Gonzalez A, 2012, PLANT SIGNAL BEHAV, V7, P728, DOI 10.4161/psb.20355; Gonzalez A, 2012, PLANT PHYSIOL, V158, P1451, DOI 10.1104/pp.111.191759; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; Greco M, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0096470; Hellwig N, 2005, J CELL SCI, V118, P917, DOI 10.1242/jcs.01675; HERBERT JM, 1990, BIOCHEM BIOPH RES CO, V172, P993, DOI 10.1016/0006-291X(90)91544-3; Hofmann T, 2002, P NATL ACAD SCI USA, V99, P7461, DOI 10.1073/pnas.102596199; KASE H, 1987, BIOCHEM BIOPH RES CO, V142, P436, DOI 10.1016/0006-291X(87)90293-2; Li SL, 2006, MOL BIOL EVOL, V23, P663, DOI 10.1093/molbev/msj075; LIU J, 1991, CELL, V66, P807, DOI 10.1016/0092-8674(91)90124-H; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Madrid R., 2015, TRP CHANNELS SENSORY, DOI [10.1007/978-3-319-18705-1, DOI 10.1007/978-3-319-18705-1]; MAGGI CA, 1993, BRIT J PHARMACOL, V108, P801, DOI 10.1111/j.1476-5381.1993.tb12881.x; Miller M, 2011, J BIOL CHEM, V286, P33436, DOI 10.1074/jbc.M111.274167; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; Nakamura Y, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0057122; Nilius B., 2014, TRANSIENT RECEPTOR P, DOI [10.1007/978-3-642-54215-2, DOI 10.1007/978-3-642-54215-2]; Provasoli L, 1974, ALGAL PHYSL BIOCH, P741; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Saez CA, 2015, PHYCOLOGIA, V54, P425, DOI 10.2216/15-30.1; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; TOKUMITSU H, 1990, J BIOL CHEM, V265, P4315; Verret F, 2010, NEW PHYTOL, V187, P23, DOI 10.1111/j.1469-8137.2010.03271.x	41	8	8	0	11	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER	ISSY-LES-MOULINEAUX	65 RUE CAMILLE DESMOULINS, CS50083, 92442 ISSY-LES-MOULINEAUX, FRANCE	0981-9428			PLANT PHYSIOL BIOCH	Plant Physiol. Biochem.	MAY	2018	126						106	116		10.1016/j.plaphy.2018.02.032			11	Plant Sciences	Plant Sciences	GD8QL	WOS:000430777100012	29518656				2021-04-07	
J	Gonzalez, A; Saez, CA; Moenne, A				Gonzalez, Alberto; Saez, Claudio A.; Moenne, Alejandra			Copper-induced activation of TRPs and VDCCs triggers a calcium signature response regulating gene expression in Ectocarpus siliculosus	PEERJ			English	Article						B Calcium; Copper; Gene expression; Voltage-dependent calcium channels; Transient receptor potential channels	DEPENDENT PROTEIN-KINASES; ULVA-COMPRESSA; CA2+ RELEASE; STRESS RESPONSES; HIGHER-PLANTS; CHANNELS; ANTIOXIDANT; ANTAGONIST; INHIBITION; CALMODULIN	certain multicellular photoautotrophs, such as plants and green macroalgae, it has been demonstrated that calcium signaling importantly mediates tolerance to copper excess. However, there is no information in brown macroalgae, which are phylogenetically distant from green algae and plants. We have previously shown that chronic copper levels (2.5 mu M) activate transient receptor potential (TRP) channels in the model brown macroalga Ectocarpus siliculosus, allowing extracellular calcium entry at 13, 29, 39 and 51 min. Here, we showed that intracellular calcium increases also occurred at 3 and 5 h of exposure; these increases were inhibited by antagonists of voltage-dependent calcium channels (VDCCs); a chelating agent of extracellular calcium; an antagonist of endoplasmic reticulum (ER) ATPase; and antagonists of cADPR-, NAADP-and IP3- dependent calcium channels. Thus, copper activates VDCCs allowing extracellular calcium entry and intracellular calcium release from the ER via cADPR-, IP3- and NAADP-dependent channels. Furthermore, the level of transcripts encoding a phytochelatin synthase (PS) and a metallothionein (MT) were analyzed in the alga exposed to 2.5 mu M copper from 3 to 24 h. The level of ps and mt transcripts increased until 24 h and these increases were inhibited by antagonists of calmodulins (CaMs), calcineurin B-like proteins (CBLs) and calcium-dependent protein kinases (CDPKs). Finally, activation of VDCC was inhibited by a mixture of TRP antagonists and by inhibitors of protein kinases. Thus, copper-mediated activation of TRPs triggers VDCCs via protein kinases, allowing extracellular calcium entry and intracellular calcium release from ER that, in turn, activate CaMs, CBLs and CDPKs increasing expression of PS and MT encoding genes in E. siliculosus.	[Gonzalez, Alberto; Moenne, Alejandra] Univ Santiago Chile, Fac Chem & Biol, Lab Marine Biotechnol, Santiago, Region Metropol, Chile; [Saez, Claudio A.] Univ Playa Ancha, Ctr Adv Studies, Lab Costal Environm Res, Valparaiso, Chile	Gonzalez, A; Moenne, A (corresponding author), Univ Santiago Chile, Fac Chem & Biol, Lab Marine Biotechnol, Santiago, Region Metropol, Chile.	alberto.gonzalezfi@usach.cl; alejandra.moenne@usach.cl	Saez, Claudio/F-5978-2015; Figueroa, Alberto Gonzalez/AAA-7911-2020	Saez, Claudio/0000-0002-5037-3484; Figueroa, Alberto Gonzalez/0000-0002-5777-9022; Moenne, Alejandra/0000-0002-7309-9713	Comision Nacional de Ciencia y Tecnologia (CONICYT) Postdoctoral Project [3150440]	This work was funded by Comision Nacional de Ciencia y Tecnologia (CONICYT) Postdoctoral Project 3150440. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Almeida MC, 2012, J NEUROSCI, V32, P2086, DOI 10.1523/JNEUROSCI.5606-11.2012; Batistic O, 2009, BBA-MOL CELL RES, V1793, P985, DOI 10.1016/j.bbamcr.2008.10.006; BISWAS S, 1995, BIOCHEM J, V306, P631, DOI 10.1042/bj3060631; Brayden JE, 2008, CLIN EXP PHARMACOL P, V35, P1116, DOI 10.1111/j.1440-1681.2007.04855.x; Butt E, 1995, BRIT J PHARMACOL, V116, P3110, DOI 10.1111/j.1476-5381.1995.tb15112.x; Catterall WA, 2011, CSH PERSPECT BIOL, V3, DOI 10.1101/cshperspect.a003947; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2002, PLANT CELL, V14, P2369, DOI 10.1105/tpc.003285; D'Angelo C, 2006, PLANT J, V48, P857, DOI 10.1111/j.1365-313X.2006.02921.x; Edel KH, 2017, CURR BIOL, V27, pR667, DOI 10.1016/j.cub.2017.05.020; Eid SR, 2008, MOL PAIN, V4, DOI 10.1186/1744-8069-4-48; Gomez M, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.00754; Gomez M, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00182; Gonzalez A, 2018, PLANT PHYSIOL BIOCH, V126, P106, DOI [10.1016/j.plaphy.2018.02.032, 10.1016/j.]; Gonzalez A, 2012, PLANT SIGNAL BEHAV, V7, P728, DOI 10.4161/psb.20355; Gonzalez A, 2012, PLANT PHYSIOL, V158, P1451, DOI 10.1104/pp.111.191759; Gonzalez A, 2010, PLANT SIGNAL BEHAV, V5, P1647, DOI 10.4161/psb.5.12.13977; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; Greco M, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0096470; HERBERT JM, 1990, BIOCHEM BIOPH RES CO, V172, P993, DOI 10.1016/0006-291X(90)91544-3; HIDAKA H, 1981, P NATL ACAD SCI-BIOL, V78, P4354, DOI 10.1073/pnas.78.7.4354; Hung WC, 2005, PLANT GROWTH REGUL, V45, P233, DOI 10.1007/s10725-005-1435-3; JOHNSON WT, 1993, BIOCHIM BIOPHYS ACTA, V1175, P263, DOI 10.1016/0167-4889(93)90215-B; KASE H, 1987, BIOCHEM BIOPH RES CO, V142, P436, DOI 10.1016/0006-291X(87)90293-2; Kilian J, 2007, PLANT J, V50, P347, DOI 10.1111/j.1365-313X.2007.03052.x; Kim MC, 2009, MOL PLANT, V2, P13, DOI 10.1093/mp/ssn091; Kudla J, 2018, NEW PHYTOL, V218, P414, DOI 10.1111/nph.14966; Laporte D, 2016, AQUAT TOXICOL, V177, P433, DOI 10.1016/j.aquatox.2016.06.017; Laver DR, 2007, BIOPHYS J, V92, P3541, DOI 10.1529/biophysj.106.099028; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lee HC, 1997, PHYSIOL REV, V77, P1133; LIU J, 1991, CELL, V66, P807, DOI 10.1016/0092-8674(91)90124-H; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Madrid R, 2015, TRP CHANNELS SENSORY, DOI [10.1074/jbc.M111.274167, DOI 10.1074/JBC.M111.274167]; MAGGI CA, 1993, BRIT J PHARMACOL, V108, P801, DOI 10.1111/j.1476-5381.1993.tb12881.x; Mao JJ, 2016, GENES-BASEL, V7, DOI 10.3390/genes7090062; MEISSNER G, 1986, J BIOL CHEM, V261, P6300; Miller M, 2011, J BIOL CHEM, V286, P33436, DOI 10.1074/jbc.M111.274167; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Muir SR, 1996, FEBS LETT, V395, P39, DOI 10.1016/0014-5793(96)01000-9; Muir SR, 1997, PLANT PHYSIOL, V114, P1511, DOI 10.1104/pp.114.4.1511; Navazio L, 2000, P NATL ACAD SCI USA, V97, P8693, DOI 10.1073/pnas.140217897; Naylor E, 2009, NAT CHEM BIOL, V5, P220, DOI 10.1038/nchembio.150; Nilius B, 2014, TRANSIENT RECEPTOR P; Perfus-Barbeoch L, 2002, PLANT J, V32, P539, DOI 10.1046/j.1365-313X.2002.01442.x; Provasoli L, 1974, VITAMINS GROWTH REGU, P741, DOI [DOI 10.1152/PHYSREV.00004.2005, 10.1152/physrev.00004.2005]; Pu RS, 1998, J CELL SCI, V111, P3197; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Rizzuto R, 2006, PHYSIOL REV, V86, P369, DOI 10.1152/physrev.00004.2005; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Saez CA, 2015, PHYCOLOGIA, V54, P425, DOI 10.2216/15-30.1; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Stael S, 2012, J EXP BOT, V63, P1525, DOI 10.1093/jxb/err394; Straub T, 2017, PLANT CELL, V29, P409, DOI 10.1105/tpc.16.00806; Tanramluk D, 2009, CHEM BIOL DRUG DES, V74, P16, DOI 10.1111/j.1747-0285.2009.00832.x; TOKUMITSU H, 1990, J BIOL CHEM, V265, P4315; Triggle DJ, 2006, CURR PHARM DESIGN, V12, P443, DOI 10.2174/138161206775474503; Valmonte GR, 2014, PLANT CELL PHYSIOL, V55, P551, DOI 10.1093/pcp/pct200; Vassilev PM, 2001, BIOCHEM BIOPH RES CO, V280, P145, DOI 10.1006/bbrc.2000.4110; Wernimont AK, 2010, NAT STRUCT MOL BIOL, V17, P596, DOI 10.1038/nsmb.1795; White PJ, 2000, BBA-BIOMEMBRANES, V1465, P171, DOI 10.1016/S0005-2736(00)00137-1; Zhu XY, 2015, TRENDS PLANT SCI, V20, P483, DOI 10.1016/j.tplants.2015.05.010	63	3	3	1	4	PEERJ INC	LONDON	341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND	2167-8359			PEERJ	PeerJ	APR 16	2018	6								e4556	10.7717/peerj.4556			20	Multidisciplinary Sciences	Science & Technology - Other Topics	GD2BW	WOS:000430306100001	29682409	DOAJ Gold, Green Published			2021-04-07	
J	Zhang, LA; Cui, CJ; Li, Y; Wu, H; Li, XJ				Zhang, Linan; Cui, Cuiju; Li, Yan; Wu, Hao; Li, Xiaojie			A genome screen for the development of sex-specific DNA markers in Saccharina japonica	JOURNAL OF APPLIED PHYCOLOGY			English	Article						Saccharina japonica; Phaeophyta; Sex-specific marker; Gametophytes; Partheno-sporophytes	BROWN ALGA ECTOCARPUS; SITE-ASSOCIATED DNA; TRIAL CULTIVATION; MALE GAMETOPHYTE; LINKAGE MAP; IDENTIFICATION; FEMALE; PHAEOPHYTA; CONSTRUCTION; LAMINARIALES	Saccharina japonica is an important cultured marine brown alga and it has been the subject of intensive genetic improvement during its domestication and farming. However, there are limited credible and effective sex markers to identify the sex of gametophytes and sporophytes derived from normal hybridization or monogenesis. In the present study, the transcriptomic and genomic sequences of S. japonica were screened by 15 sex-determining region (SDR) genes from a model brown alga (Ectocarpus), and six cDNA sequences and their corresponding genomic sequences were identified. Thirteen primer pairs were designed from the coding region of six genomic sequences and subsequently tested on gametophytes of S. japonica. From these regions, four novel male-specific markers were developed, which derived from S. japonica genomic sequences JXRI01001736 and JXRI01002827, corresponding to a male-specific SDR gene and a gametologue SDR gene in Ectocarpus, respectively. In addition, an existing female marker (M_68_58_2) for Macrocystis pyrifera and Undaria pinnatifida, derived from the Ectocarpus SDR gene, could also be used as a female-specific marker for S. japonica. The results of the present study not only demonstrate an important new tool to determine the sex of gametophytes and distinguish between diploid sporophytes and partheno-sporophytes in breeding programmes but also provide insight into the sex-determination system for S. japonica, including the identification of sex chromosomes, SDR, and SDR genes and evolution of sex chromosomes.	[Zhang, Linan] Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China; [Cui, Cuiju; Li, Yan; Li, Xiaojie] Shandong Oriental Ocean Sci tech Co Ltd, Natl Engn Sci Res & Dev Ctr Algae & Sea Cucumbers, Prov Key Lab Genet Improvement & Efficient Cultur, Yantai 264003, Peoples R China; [Wu, Hao] Qingdao Agr Univ, Coll Food Sci & Engn, Qingdao 266109, Peoples R China	Li, XJ (corresponding author), Shandong Oriental Ocean Sci tech Co Ltd, Natl Engn Sci Res & Dev Ctr Algae & Sea Cucumbers, Prov Key Lab Genet Improvement & Efficient Cultur, Yantai 264003, Peoples R China.; Wu, H (corresponding author), Qingdao Agr Univ, Coll Food Sci & Engn, Qingdao 266109, Peoples R China.	wuhaoqau@163.com; yeslxj@sina.com	Wu, Hao/AAP-5943-2020		National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41606184]; Shandong Province Higher Educational Science and Technology Program [J15LE14]; Qingdao Applied Basic Research Project [14-2-4-71-jch]; Shandong Province Agriculture Seed Project [2016LZGC024]; Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences [CXGC2016B10]	This study was supported by the National Natural Sciences Foundation of China (Grant No.: 41606184), Shandong Province Higher Educational Science and Technology Program (Grant No.: J15LE14), Qingdao Applied Basic Research Project (Grant No.: 14-2-4-71-jch), Shandong Province Agriculture Seed Project (Grand No.: 2016LZGC024), and Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (Grant No.: CXGC2016B10).	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Bello N, 1999, MOL ECOL, V8, P667, DOI 10.1046/j.1365-294x.1999.00549.x; BULL JJ, 1978, AM NAT, V112, P245, DOI 10.1086/283267; Chen SL, 2007, MAR BIOTECHNOL, V9, P273, DOI 10.1007/s10126-006-6081-x; Chen SL, 2012, MAR BIOTECHNOL, V14, P120, DOI 10.1007/s10126-011-9395-2; Dai JX., 1976, ACTA GENET SIN, V3, P32; Elmeer K, 2012, 3 BIOTECH, V2, P241, DOI 10.1007/s13205-012-0052-x; Fang TC., 1962, ACTA BOT SIN, V10, P197; Fang Z., 1978, T SCI, V2, P115; Fowler BLS, 2016, MOL ECOL, V25, P2165, DOI 10.1111/mec.13594; Gamble T, 2014, MOL ECOL RESOUR, V14, P902, DOI 10.1111/1755-0998.12237; Griffiths R, 2000, J FISH BIOL, V57, P1331, DOI 10.1111/j.1095-8649.2000.tb00490.x; Griffiths R, 1999, MOL ECOL, V8, P671, DOI 10.1046/j.1365-294x.1999.00578.x; Koressaar T, 2007, BIOINFORMATICS, V23, P1289, DOI 10.1093/bioinformatics/btm091; Korpelainen H, 2008, J HERED, V99, P581, DOI 10.1093/jhered/esn036; Lamatsch DK, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0118214; Lee BY, 2003, ANIM GENET, V34, P379, DOI 10.1046/j.1365-2052.2003.01035.x; Li XJ, 2008, AQUACULTURE, V280, P76, DOI 10.1016/j.aquaculture.2008.05.005; Li XJ, 2007, J APPL PHYCOL, V19, P139, DOI 10.1007/s10811-006-9120-0; Li XJ, 2016, SCI REP-UK, V6, DOI 10.1038/srep21255; Li YH, 2017, PLOS ONE, V12, DOI 10.1371/journal.pone.0171187; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Lipinska AP, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140535; Liu F, 2015, J APPL PHYCOL, V27, P469, DOI 10.1007/s10811-014-0295-5; Liu YS, 2009, J PHYCOL, V45, P894, DOI 10.1111/j.1529-8817.2009.00719.x; Liu Y, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0048784; Petrella V, 2015, BMC GENOMICS, V16, DOI 10.1186/s12864-015-2088-x; Scoggan J, 1989, LAMINARIA SEAFARMING; Sim MC, 2007, J APPL PHYCOL, V19, P763, DOI 10.1007/s10811-007-9224-1; Stehlik I, 2004, THEOR APPL GENET, V108, P238, DOI 10.1007/s00122-003-1425-7; Tian ZP., 1989, MARINE AQUACULTURE, V1, P7; Tseng CK, 2001, J APPL PHYCOL, V13, P375, DOI 10.1023/A:1017972812576; Untergasser A, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gks596; YABU H, 1991, Japanese Journal of Phycology, V39, P185; Yang GP, 2009, J PHYCOL, V45, P873, DOI 10.1111/j.1529-8817.2009.00720.x; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zhang LA, 2013, J APPL PHYCOL, V25, P261, DOI 10.1007/s10811-012-9860-y; Zhang N, 2015, BMC GENOMICS, V16, DOI 10.1186/s12864-015-1371-1; Zhang QS, 2007, J APPL PHYCOL, V19, P303, DOI 10.1007/s10811-006-9137-4; Zhou LR, 2004, HYDROBIOLOGIA, V512, P141, DOI 10.1023/B:HYDR.0000020319.36532.eb	41	4	4	3	19	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0921-8971	1573-5176		J APPL PHYCOL	J. Appl. Phycol.	APR	2018	30	2					1239	1246		10.1007/s10811-017-1295-z			8	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	GE4OG	WOS:000431194900045					2021-04-07	
J	Celis-Pla, PSM; Brown, MT; Santillan-Sarmiento, A; Korbee, N; Saez, CA; Figueroa, FL				Celis-Pla, Paula S. M.; Brown, Murray T.; Santillan-Sarmiento, Alex; Korbee, Nathalie; Saez, Claudio A.; Figueroa, Felix L.			Ecophysiological and metabolic responses to interactive exposure to nutrients and copper excess in the brown macroalga Cystoseira tamariscifolia	MARINE POLLUTION BULLETIN			English	Article						Copper; Cystoseira tamariscifolia; Nutrient; In vivo chlorophyll a; Phenolic compounds; Antioxidant capacity	DIFFERENT POLLUTION HISTORIES; ALGA FUCUS-VESICULOSUS; CHLOROPHYLL FLUORESCENCE; ECTOCARPUS-SILICULOSUS; BIOSYNTHETIC-PATHWAY; MARINE MACROALGAE; AMBIENT SALINITY; PHAEOPHYCEAE; PHOTOSYNTHESIS; STRESS	Global scenarios evidence that contamination due to anthropogenic activities occur at different spatial-temporal scales, being important stressors: eutrophication, due to increased nutrient inputs; and metal pollution, mostly derived from industrial activities. In this study, we investigated ecophysiological and metabolic responses to copper and nutrient excess in the brown macroalga Cystoseira tamariscifolia. Whole plants were incubated in an indoor system under control conditions, two levels of nominal copper (0.5 and 2.0 mu M), and two levels of nutrient supply for two weeks. Maximal quantum yield (F-v/F-m) and maximal electron transport rate (ETRmax) increased under copper exposure. Photosynthetic pigments and phenolic compounds (PC) increased under the highest copper levels. The intra-cellular copper content increased under high copper exposure in both nutrient conditions. C. tamariscifolia from the Atlantic displayed efficient metal exclusion mechanisms, since most of the total copper accumulated by the cell was bound to the cell wall.	[Celis-Pla, Paula S. M.; Saez, Claudio A.] Univ Playa Ancha, Ctr Adv Studies, Lab Coastal Environm Res, Traslavina 450, Vina Del Mar 581782, Chile; [Celis-Pla, Paula S. M.; Korbee, Nathalie; Figueroa, Felix L.] Univ Malaga, Fac Sci, Dept Ecol & Geol, Malaga 29071, Spain; [Brown, Murray T.; Santillan-Sarmiento, Alex] Plymouth Univ, Sch Marine Sci & Engn, Drake Circus, Plymouth PL4 8AA, Devon, England	Celis-Pla, PSM (corresponding author), Univ Playa Ancha, Ctr Adv Studies, Lab Coastal Environm Res, Traslavina 450, Vina Del Mar 581782, Chile.	paulacelispla@upla.cl	Celis-Pla, Paula S.M./I-9123-2016; Saez, Claudio/F-5978-2015; Korbee, Nathalie/K-5995-2014; Brown, Murray/K-5291-2014; Lopez Figueroa, Felix Diego/K-7720-2014	Celis-Pla, Paula S.M./0000-0002-2247-3787; Saez, Claudio/0000-0002-5037-3484; Korbee, Nathalie/0000-0002-9780-4915; Brown, Murray/0000-0003-2655-8611; Lopez Figueroa, Felix Diego/0000-0003-3580-4693; Santillan-Sarmiento, Alex R./0000-0003-4663-0959	Junta de AndaluciaJunta de Andalucia [RNM-5750, RNM-295]; "Becas-Chile Doctorado" (CONICYT) fellowship of the Ministry of Education of the Republic of Chile [72110192]	This work was supported by the Junta de Andalucia, Project No RNM-5750 of the research group RNM-295. Paula S. M. Cells-Pla gratefully acknowledges financial support from "Becas-Chile Doctorado No 72110192" (CONICYT) fellowship of the Ministry of Education of the Republic of Chile to conduct PhD studied. We thank technical support to all involved technicians at Plymouth University, especially to Dr. William Vevers.	Anderson M.J., 2008, PERMANOVA PRIMER GUI; Andrade S, 2006, AQUAT TOXICOL, V78, P398, DOI 10.1016/j.aquatox.2006.04.006; Audibert L, 2010, PHYTOCHEM ANALYSIS, V21, P399, DOI 10.1002/pca.1210; Bunker FStP, 2010, SEA SEARCH GUIDE SEA; Celis-Pla PSM, 2014, AQUAT BIOL, V22, P227, DOI 10.3354/ab00573; Celis-Pla P, 2017, CLIMATIC CHANGE, V142, P67, DOI 10.1007/s10584-017-1943-y; Celis-Pla PSM, 2016, MAR ENVIRON RES, V115, P89, DOI 10.1016/j.marenvres.2015.11.014; Celis-Pla PSM, 2014, SCI MAR, V78, P377, DOI 10.3989/scimar.04053.05A; Connan S, 2011, AQUAT TOXICOL, V104, P94, DOI 10.1016/j.aquatox.2011.03.015; Connan S, 2011, AQUAT TOXICOL, V104, P1, DOI 10.1016/j.aquatox.2011.03.016; Costa GB, 2016, PROTOPLASMA, V253, P111, DOI 10.1007/s00709-015-0795-4; Davis TA, 2003, WATER RES, V37, P4311, DOI 10.1016/S0043-1354(03)00293-8; EILERS PHC, 1988, ECOL MODEL, V42, P199, DOI 10.1016/0304-3800(88)90057-9; Ferreira JG, 2011, ESTUAR COAST SHELF S, V93, P117, DOI 10.1016/j.ecss.2011.03.014; Figueroa FL, 2009, AQUAT BIOL, V7, P159, DOI 10.3354/ab00186; Figueroa FL, 2003, PHOTOSYNTH RES, V75, P259, DOI 10.1023/A:1023936313544; Garcia-Sanchez M, 2014, MAR ENVIRON RES, V101, P8, DOI 10.1016/j.marenvres.2014.07.012; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; Gomez-Garreta A., 2001, FLORA PHYCOLOGICA IB; Graham MH, 2007, P NATL ACAD SCI USA, V104, P16576, DOI 10.1073/pnas.0704778104; Grzymski J, 1997, J PHYCOL, V33, P408, DOI 10.1111/j.0022-3646.1997.00408.x; Hassler CS, 2004, LIMNOL OCEANOGR-METH, V2, P237, DOI 10.4319/lom.2004.2.237; Huovinen P, 2010, MAR FRESHWATER RES, V61, P330, DOI 10.1071/MF09054; Koivikko R, 2007, PHYTOCHEM ANALYSIS, V18, P326, DOI 10.1002/pca.986; Kupper H, 2002, J PHYCOL, V38, P429, DOI 10.1046/j.1529-8817.2002.01148.x; LITTLER MM, 1984, J EXP MAR BIOL ECOL, V74, P13, DOI 10.1016/0022-0981(84)90035-2; Martinez B, 2012, OECOLOGIA, V170, P341, DOI 10.1007/s00442-012-2324-x; Mikami K, 2013, INT J MOL SCI, V14, P13763, DOI 10.3390/ijms140713763; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Nielsen HD, 2010, MAR POLLUT BULL, V60, P710, DOI 10.1016/j.marpolbul.2009.11.025; Pfister CA, 2003, J PHYCOL, V39, P285, DOI 10.1046/j.1529-8817.2003.01229.x; Ramirez T, 2005, ESTUAR COAST SHELF S, V65, P654, DOI 10.1016/j.ecss.2005.07.012; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Ryan S, 2012, ENVIRON POLLUT, V167, P171, DOI 10.1016/j.envpol.2012.04.006; Sachindra NM, 2007, J AGR FOOD CHEM, V55, P8516, DOI 10.1021/jf071848a; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; SCHREIBER U, 1995, PLANT CELL PHYSIOL, V36, P873, DOI 10.1093/oxfordjournals.pcp.a078833; SEELY GR, 1972, MAR BIOL, V12, P184, DOI 10.1007/BF00350754; Underwood T., 1997, EXPT ECOLOGY THEIR L; Van Alstyne KL, 2000, MAR ECOL PROG SER, V206, P33, DOI 10.3354/meps206033; Wells E, 2007, MAR POLLUT BULL, V55, P151, DOI 10.1016/j.marpolbul.2006.08.031; Woodward E. M. S., 2013, PML BENTHIC SURVEY W; Wurch LL, 2014, ENVIRON MICROBIOL, V16, P2444, DOI 10.1111/1462-2920.12374; Xiong J, 2010, ALLELOPATHY J, V25, P345	46	11	11	3	19	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND	0025-326X	1879-3363		MAR POLLUT BULL	Mar. Pollut. Bull.	MAR	2018	128						214	222		10.1016/j.marpolbul.2018.01.005			9	Environmental Sciences; Marine & Freshwater Biology	Environmental Sciences & Ecology; Marine & Freshwater Biology	GD6VC	WOS:000430645600025	29571366				2021-04-07	
J	Valdes, FA; Lobos, MG; Diaz, P; Saez, CA				Valdes, Felipe A.; Gabriela Lobos, M.; Diaz, Patricia; Saez, Claudio A.			Metal assessment and cellular accumulation dynamics in the green macroalga Ulva lactuca	JOURNAL OF APPLIED PHYCOLOGY			English	Article						Seaweed; Metal tolerance; Chlorophyta; Cell wall; Metal pollution	DIFFERENT POLLUTION HISTORIES; PHYSIOLOGICAL-RESPONSES; ECTOCARPUS-SILICULOSUS; COPPER ACCUMULATION; NORTHERN CHILE; TRP CHANNELS; COMPRESSA; CHLOROPHYTA; ACTIVATION; CALCIUM	We have tested Ulva lactuca Linnaeus as a metal biomonitor by measuring the concentrations of metals copper (Cu), cadmium (Cd), zinc (Zn), and mercury (Hg) in its biomass and in nearby sediments, within impacted and non-impacted sites in central Chile. Moreover, through Cu exposure laboratory experiments, we observed the dynamics of intracellular and extracellular Cu accumulation in two populations of U. lactuca. Highest metal levels in U. lactuca were associated with human activities; the exception was Cd, with high levels in the control site, although this could be explained due to the important nearby upwelling conditions. Laboratory experiments showed that more of the 90% of the total Cu in the two populations of U. lactuca was accumulated intracellularly, without intra-specific differences. Biomonitoring with U. lactuca provides a positive representation of metal status, which is reinforced in combination with metal measurements in sediments. Weak patterns in metal exclusion suggest the use of different strategies to counteract metal excess in U. lactuca, for instance, associated with the syntheses of metal chelators and the antioxidant metabolism.	[Valdes, Felipe A.; Saez, Claudio A.] Univ Playa Ancha, Ctr Adv Studies, Lab Coastal Environm Res, Vina Del Mar, Chile; [Valdes, Felipe A.] Univ Playa Ancha, Dept Environm, Fac Engn, Valparaiso, Chile; [Gabriela Lobos, M.; Diaz, Patricia] Univ Valparaiso, Inst Chem & Biochem, Lab Analyt & Environm Chem, Valparaiso, Chile	Saez, CA (corresponding author), Univ Playa Ancha, Ctr Adv Studies, Lab Coastal Environm Res, Vina Del Mar, Chile.	claudio.saez@upla.cl	Saez, Claudio/F-5978-2015	Saez, Claudio/0000-0002-5037-3484	Convenio Desempeno UPA at the University of Playa Ancha [1301]; FONDECYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [11160369, 1150855]; FONDEQUIP [EQM120169]	We thank the financial support from project Convenio Desempeno UPA 1301 at the University of Playa Ancha to C. A. Saez. We also appreciate the budget contribution from FONDECYT project nos. 11160369 and 1150855 granted to C. A. Saez and M. G. Lobos, respectively, and project FONDEQUIP no. EQM120169 to M. G. Lobos.	ABE K, 1988, MAR CHEM, V23, P145, DOI 10.1016/0304-4203(88)90028-X; Andrade LR, 2004, ECOTOX ENVIRON SAFE, V58, P117, DOI 10.1016/S0147-6513(03)00106-4; Brown Murray T., 1998, Chapman & Hall Ecotoxicology Series, V7, P185; BRULAND KW, 1980, EARTH PLANET SC LETT, V47, P176, DOI 10.1016/0012-821X(80)90035-7; Burger J, 2007, ENVIRON MONIT ASSESS, V128, P311, DOI 10.1007/s10661-006-9314-6; Currie LA, 1999, ANAL CHIM ACTA, V391, P127, DOI 10.1016/S0003-2670(99)00105-1; Diop M, 2016, MAR POLLUT BULL, V103, P339, DOI 10.1016/j.marpolbul.2015.12.038; dos Santos RW, 2014, ECOTOX ENVIRON SAFE, V105, P80, DOI 10.1016/j.ecoenv.2014.02.021; Gaudry A, 2007, WATER AIR SOIL POLL, V178, P267, DOI 10.1007/s11270-006-9196-9; Gledhill M, 1998, MAR ENVIRON RES, V45, P295, DOI 10.1016/S0141-1136(98)00100-7; Gomez I., 2012, ECOL STUD, V219, P293; Gomez M, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.00754; Gomez M, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00182; Gonzalez A, 2012, PLANT PHYSIOL, V158, P1451, DOI 10.1104/pp.111.191759; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; Bermudez YG, 2011, CHEM ENG J, V166, P122, DOI 10.1016/j.cej.2010.10.038; Green-Ruiz C, 2005, ENVIRON GEOCHEM HLTH, V27, P321, DOI 10.1007/s10653-004-5741-x; Huang HZ, 2013, CHIN J OCEANOL LIMN, V31, P803, DOI 10.1007/s00343-013-2261-5; Kalin M, 2005, J ENVIRON RADIOACTIV, V78, P151, DOI 10.1016/j.jenvrad.2004.05.002; Laib E, 2012, ENVIRON MONIT ASSESS, V184, P1711, DOI 10.1007/s10661-011-2072-0; Medina M, 2005, MAR POLLUT BULL, V50, P396, DOI 10.1016/j.marpolbul.2004.11.022; Mehta SK, 2000, EUR J PROTISTOL, V36, P443, DOI 10.1016/S0932-4739(00)80050-4; MINSAL, 1997, REGL SAN AL; Moenne A, 2016, AQUAT TOXICOL, V176, P30, DOI 10.1016/j.aquatox.2016.04.015; Ratkevicius N, 2003, PLANT CELL ENVIRON, V26, P1599, DOI 10.1046/j.1365-3040.2003.01073.x; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Saez CA, 2015, PHYCOLOGIA, V54, P425, DOI 10.2216/15-30.1; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Saez CA, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0050170; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; Schmidt EC, 2015, PHOTOCHEM PHOTOBIOL, V91, P359, DOI 10.1111/php.12396; Schramm W, 2012, MARINE BENTHIC VEGET; Silva N., 2003, INVEST MAR, V31, P73, DOI [10.4067/S0717-71782003000200007, DOI 10.4067/S0717-71782003000200007]; Stengel DB, 2005, ESTUAR COAST SHELF S, V65, P687, DOI 10.1016/j.ecss.2005.07.003; Taverniers I, 2004, TRAC-TREND ANAL CHEM, V23, P535, DOI 10.1016/j.trac.2004.04.001; Turner A, 2009, ENVIRON POLLUT, V157, P2314, DOI 10.1016/j.envpol.2009.03.026; Valdivia N, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0100714; Zhou JL, 1998, ENVIRON POLLUT, V101, P67, DOI 10.1016/S0269-7491(98)00034-7	39	5	5	3	22	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0921-8971	1573-5176		J APPL PHYCOL	J. Appl. Phycol.	FEB	2018	30	1					663	671		10.1007/s10811-017-1244-x			9	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	FZ7SY	WOS:000427804800065					2021-04-07	
J	KleinJan, H; Jeanthon, C; Boyen, C; Dittami, SM				KleinJan, Hetty; Jeanthon, Christian; Boyen, Catherine; Dittami, Simon M.			Exploring the Cultivable Ectocarpus Microbiome	FRONTIERS IN MICROBIOLOGY			English	Article						Ectocarpus; holobiont; bacterial cultivation; brown macroalgae; dilution-to-extinction; metabarcoding	MARINE-BACTERIA; PHYLOGENETIC ANALYSIS; ALGINATE LYASE; DIVERSITY; ALGAE; WATER; EVOLUTION; CULTURE; GROWTH; SEA	Coastal areas form the major habitat of brown macroalgae, photosynthetic multicellular eukaryotes that have great ecological value and industrial potential. Macroalgal growth, development, and physiology are influenced by the microbial community they accommodate. Studying the algal microbiome should thus increase our fundamental understanding of algal biology and may help to improve culturing efforts. Currently, a freshwater strain of the brown macroalga Ectocarpus subulatus is being developed as a model organism for brown macroalgal physiology and algal microbiome studies. It can grow in high and low salinities depending on which microbes it hosts. However, the molecular mechanisms involved in this process are still unclear. Cultivation of Ectocarpus-associated bacteria is the first step toward the development of a model system for in vitro functional studies of brown macroalgal-bacterial interactions during abiotic stress. The main aim of the present study is thus to provide an extensive collection of cultivable E. subulatus-associated bacteria. To meet the variety of metabolic demands of Ectocarpus-associated bacteria, several isolation techniques were applied, i.e., direct plating and dilution-to-extinction cultivation techniques, each with chemically defined and undefined bacterial growth media. Algal tissue and algal growth media were directly used as inoculum, or they were pretreated with antibiotics, by filtration, or by digestion of algal cell walls. In total, 388 isolates were identified falling into 33 genera (46 distinct strains), of which Halomonas (Gammaproteobacteria), Bosea (Alphaproteobacteria), and Limnobacter (Betaproteobacteria) were the most abundant. Comparisons with 16S rRNA gene metabarcoding data showed that culturability in this study was remarkably high (similar to 50%), although several cultivable strains were not detected or only present in extremely low abundance in the libraries. These undetected bacteria could be considered as part of the rare biosphere and they may form the basis for the temporal changes in the Ectocarpus microbiome.	[KleinJan, Hetty; Boyen, Catherine; Dittami, Simon M.] Sorbonne Univ, CNRS UPMC, Stn Biol Roscoff, UMR8227,Integrat Biol Marine Models, Roscoff, France; [Jeanthon, Christian] CNRS, Stn Biol Roscoff, UMR7144, Adaptat & Diversite Milieu Marin, Roscoff, France; [Jeanthon, Christian] UPMC Univ Paris 06, Sorbonne Univ, Stn Biol Roscoff, UMR7144,Adaptat & Diversite Milieu Marin, Roscoff, France	KleinJan, H; Dittami, SM (corresponding author), Sorbonne Univ, CNRS UPMC, Stn Biol Roscoff, UMR8227,Integrat Biol Marine Models, Roscoff, France.	hetty.kleinjan@sb-roscoff.fr; simon.dittami@sb-rocoff.fr		Jeanthon, Christian/0000-0002-7339-574X	European Union's Horizon research and innovation programme under the Marie Sklodowska-Curie grant [624575]; ANR project IDEALGFrench National Research Agency (ANR) [ANR-10-BTBR-04]	This work has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement number 624575 (ALFF) and ANR project IDEALG (ANR-10-BTBR-04) "Investissements d'Avenir, Biotechnologies-Bioressources".	AMANN RI, 1995, MICROBIOL REV, V59, P143, DOI 10.1128/MMBR.59.1.143-169.1995; Arahal DR, 2006, PROKARYOTES: A HANDBOOK ON THE BIOLOGY OF BACTERIA, VOL 6, THIRD EDITION, P811, DOI 10.1007/0-387-30746-x_28; Baggesen C., 2014, THESIS; Bengtsson MM, 2010, AQUAT MICROB ECOL, V60, P71, DOI 10.3354/ame01409; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Buerger S, 2012, APPL ENVIRON MICROB, V78, P3221, DOI 10.1128/AEM.07307-11; Burke C, 2011, ISME J, V5, P590, DOI 10.1038/ismej.2010.164; BUTTON DK, 1993, APPL ENVIRON MICROB, V59, P881, DOI 10.1128/AEM.59.3.881-891.1993; Carini P, 2013, ISME J, V7, P592, DOI 10.1038/ismej.2012.122; Cho JC, 2004, APPL ENVIRON MICROB, V70, P432, DOI 10.1128/AEM.70.1.432-440.2004; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P361, DOI 10.1101/pdb.prot067959; Connon SA, 2002, APPL ENVIRON MICROB, V68, P3878, DOI 10.1128/AEM.68.8.3878-3885.2002; Croft MT, 2005, NATURE, V438, P90, DOI 10.1038/nature04056; Dittami SM, 2017, J PHYCOL, V53, P731, DOI 10.1111/jpy.12547; Dittami SM, 2016, ISME J, V10, P51, DOI 10.1038/ismej.2015.104; Dittami SM, 2014, MOL ECOL, V23, P1656, DOI 10.1111/mec.12670; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Donachie SP, 2004, MICROB ECOL, V48, P509, DOI 10.1007/s00248-004-0217-1; Donachie SP, 2007, ISME J, V1, P97, DOI 10.1038/ismej.2007.22; Dong S, 2012, MAR DRUGS, V10, P2481, DOI 10.3390/md10112481; Edgar RC, 2011, BIOINFORMATICS, V27, P2194, DOI 10.1093/bioinformatics/btr381; Egan S, 2013, FEMS MICROBIOL REV, V37, P462, DOI 10.1111/1574-6976.12011; Eilers H, 2000, APPL ENVIRON MICROB, V66, P3044, DOI 10.1128/AEM.66.7.3044-3051.2000; Epstein SS, 2009, NATURE, V457, P1083, DOI 10.1038/4571083a; Esteves AIS, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.00499; Falcone-Dias MF, 2012, WATER RES, V46, P3612, DOI 10.1016/j.watres.2012.04.007; Ficko-Blean E, 2015, PERSPECT PHYCOL, V2, P51, DOI [DOI 10.1127/PIP/2015/0028, 10.1127/pip/2015/0028]; Garza DR, 2015, CELL MOL LIFE SCI, V72, P4287, DOI 10.1007/s00018-015-2004-1; Giovannoni SJ, 2005, NATURE, V437, P343, DOI 10.1038/nature04158; Goecke F, 2013, EUR J PHYCOL, V48, P47, DOI 10.1080/09670262.2013.767944; Goecke F, 2013, PHYCOLOGIA, V52, P14, DOI 10.2216/12-24.1; Goecke F, 2012, REV BIOL MAR OCEANOG, V47, P75, DOI 10.4067/S0718-19572012000100007; Goecke F, 2010, MAR ECOL PROG SER, V409, P267, DOI 10.3354/meps08607; Groisillier A, 2015, APPL ENVIRON MICROB, V81, P1790, DOI 10.1128/AEM.02808-14; Hollants J, 2013, FEMS MICROBIOL ECOL, V83, P1, DOI 10.1111/j.1574-6941.2012.01446.x; Ivanova EP, 2002, MICROB ECOL, V43, P242, DOI 10.1007/s00248-001-1011-y; Jimenez-Infante F, 2014, FEMS MICROBIOL ECOL, V89, P181, DOI 10.1111/1574-6941.12348; Jousset A, 2017, ISME J, V11, P853, DOI 10.1038/ismej.2016.174; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Keller M, 2004, NAT REV MICROBIOL, V2, P141, DOI 10.1038/nrmicro819; KESHTACHERLIEBSON E, 1995, APPL ENVIRON MICROB, V61, P2439, DOI 10.1128/AEM.61.6.2439-2441.1995; KONG MK, 1979, BOT MAR, V22, P83, DOI 10.1515/botm.1979.22.2.83; Kozich JJ, 2013, APPL ENVIRON MICROB, V79, P5112, DOI 10.1128/AEM.01043-13; Lagier JC, 2012, CLIN MICROBIOL INFEC, V18, P1185, DOI 10.1111/1469-0691.12023; Lavy A, 2014, FEMS MICROBIOL ECOL, V87, P486, DOI 10.1111/1574-6941.12240; Letunic I, 2016, NUCLEIC ACIDS RES, V44, pW242, DOI 10.1093/nar/gkw290; Lindh MV, 2015, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.00223; Lu HS, 2011, INT J SYST EVOL MICR, V61, P404, DOI 10.1099/ijs.0.020206-0; Mancuso FP, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.00476; Marie D, 1997, APPL ENVIRON MICROB, V63, P186, DOI 10.1128/AEM.63.1.186-193.1997; Martin M, 2015, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.01487; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Morris RM, 2002, NATURE, V420, P806, DOI 10.1038/nature01240; Page KA, 2004, APPL ENVIRON MICROB, V70, P6542, DOI 10.1128/AEM.70.11.6542-6550.2004; Parrot D, 2015, SCI REP-UK, V5, DOI 10.1038/srep15839; PEDERSEN M, 1973, PHYSIOL PLANTARUM, V28, P101, DOI 10.1111/j.1399-3054.1973.tb01158.x; PEDERSEN M, 1969, PHYSIOL PLANTARUM, V22, P977, DOI 10.1111/j.1399-3054.1969.tb07455.x; Pedros-Alio C, 2012, ANNU REV MAR SCI, V4, P449, DOI 10.1146/annurev-marine-120710-100948; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2015, CRYPTOGAMIE ALGOL, V36, P3, DOI 10.7872/crya.v36.iss1.2015.3; Poli A, 2009, CARBOHYD POLYM, V78, P651, DOI 10.1016/j.carbpol.2009.05.031; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Quast C, 2013, NUCLEIC ACIDS RES, V41, pD590, DOI 10.1093/nar/gks1219; REASONER DJ, 1985, APPL ENVIRON MICROB, V49, P1; RIEPERKIRCHNER M, 1989, BOT MAR, V32, P241, DOI 10.1515/botm.1989.32.3.241; RStudio Team, 2016, RSTUDIO INT DEV R; Salaun S, 2012, MICROB ECOL, V64, P359, DOI 10.1007/s00248-012-0048-4; Salaun S, 2010, TALANTA, V80, P1758, DOI 10.1016/j.talanta.2009.10.020; Sawabe T, 1997, CARBOHYD RES, V304, P69, DOI 10.1016/S0008-6215(97)00194-8; Shade A, 2015, TRENDS MICROBIOL, V23, P335, DOI 10.1016/j.tim.2015.01.007; Shade A, 2012, ENVIRON MICROBIOL, V14, P2247, DOI 10.1111/j.1462-2920.2012.02817.x; Singh RP, 2016, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.01488; Singh RP, 2014, FEMS MICROBIOL ECOL, V88, P213, DOI 10.1111/1574-6941.12297; Sipkema D, 2011, APPL ENVIRON MICROB, V77, P2130, DOI 10.1128/AEM.01203-10; Skopina MY, 2016, MICROBIOLOGY+, V85, P272, DOI 10.1134/S0026261716030139; Sogin ML, 2006, P NATL ACAD SCI USA, V103, P12115, DOI 10.1073/pnas.0605127103; Spoerner M, 2012, J PHYCOL, V48, P1433, DOI 10.1111/j.1529-8817.2012.01231.x; Spring S, 2001, INT J SYST EVOL MICR, V51, P1463, DOI 10.1099/00207713-51-4-1463; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Staufenberger T, 2008, FEMS MICROBIOL ECOL, V64, P65, DOI 10.1111/j.1574-6941.2008.00445.x; Stewart EJ, 2012, J BACTERIOL, V194, P4151, DOI 10.1128/JB.00345-12; Stingl U, 2008, MICROB ECOL, V55, P395, DOI 10.1007/s00248-007-9284-4; Stingl U, 2007, ISME J, V1, P361, DOI 10.1038/ismej.2007.49; Suzuki MT, 1996, APPL ENVIRON MICROB, V62, P625, DOI 10.1128/AEM.62.2.625-630.1996; Suzuki R, 2006, BIOINFORMATICS, V22, P1540, DOI 10.1093/bioinformatics/btl117; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tang Jingchun, 2008, International Journal of Biotechnology, V10, P73, DOI 10.1504/IJBT.2008.017970; Tapia JE, 2016, FRONT MICROBIOL, V7, DOI [10.3389/fmicb.2016.00197, 10.3389/fmicb.2016.00107]; Thornber C., 2016, MARINE MACROPHYTES F, P43, DOI [10.4324/9781315370781-4, DOI 10.4324/9781315370781-4]; Tripp HJ, 2008, NATURE, V452, P741, DOI 10.1038/nature06776; Troussellier M, 2017, FRONT MICROBIOL, V8, DOI 10.3389/fmicb.2017.00947; Vaz-Moreira I, 2013, FEMS MICROBIOL ECOL, V83, P361, DOI 10.1111/1574-6941.12002; Wahl M, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00292; Wang G, 2008, J APPL PHYCOL, V20, P403, DOI 10.1007/s10811-007-9274-4; Wang Q, 2007, APPL ENVIRON MICROB, V73, P5261, DOI 10.1128/AEM.00062-07; WEISBURG WG, 1991, J BACTERIOL, V173, P697, DOI 10.1128/JB.173.2.697-703.1991; Wells ML, 2017, J APPL PHYCOL, V29, P949, DOI 10.1007/s10811-016-0974-5; West John A., 1996, Muelleria, V9, P29; Wiese J, 2009, MAR BIOTECHNOL, V11, P287, DOI 10.1007/s10126-008-9143-4; Wong TY, 2000, ANNU REV MICROBIOL, V54, P289, DOI 10.1146/annurev.micro.54.1.289; Wu HT, 2014, BIOMED ENVIRON SCI, V27, P646, DOI 10.3967/bes2014.099; Yang SJ, 2016, MICROB ECOL, V71, P29, DOI 10.1007/s00248-015-0695-3; Zengler K, 2013, HUMAN MICROBIOTA: HOW MICROBIAL COMMUNITIES AFFECT HEALTH AND DISEASE, P289; Zhang L, 2014, CAN J MICROBIOL, V60, P319, DOI 10.1139/cjm-2013-0808; Zilber-Rosenberg I, 2008, FEMS MICROBIOL REV, V32, P723, DOI 10.1111/j.1574-6976.2008.00123.x; Zobell CE, 1941, J MAR RES, V4, P42; Zothanpuia, 2016, PEERJ, V4, DOI 10.7717/peerj.2103	108	16	16	0	12	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-302X			FRONT MICROBIOL	Front. Microbiol.	DEC 11	2017	8								2456	10.3389/fmicb.2017.02456			13	Microbiology	Microbiology	FP4HN	WOS:000417577800001	29312170	DOAJ Gold, Green Published			2021-04-07	
J	Liu, F; Jin, Z; Wang, Y; Bi, YP; Melton, JT				Liu, Feng; Jin, Zhe; Wang, Yu; Bi, Yuping; Melton, James T., III			Plastid Genome of Dictyopteris divaricata (Dictyotales, Phaeophyceae): Understanding the Evolution of Plastid Genomes in Brown Algae	MARINE BIOTECHNOLOGY			English	Article						Phaeophyceae; Dictyotales; Plastid genome; Brown alga; Inverted repeat; Evolution	HORIZONTAL GENE-TRANSFER; NEIGHBOR-JOINING METHOD; CHLOROPLAST GENOME; ORGANELLAR GENOMES; PHYLOGENETIC TREES; SEQUENCES; DIATOMS; MECHANISMS; GENERATION; ALIGNMENT	Dictyotophycidae is a subclass of brown algae containing 395 species that are distributed worldwide. A complete plastid (chloroplast) genome (ptDNA or cpDNA) had not previously been sequenced from this group. In this study, the complete plastid genome of Dictyopteris divaricata (Okamura) Okamura (Dictyotales, Phaeophyceae) was characterized and compared to other brown algal ptDNAs. This plastid genome was 126,099 bp in size with two inverted repeats (IRs) of 6026 bp. The D. divaricata IRs contained rpl21, making its IRs larger than representatives from the orders Fucales and Laminariales, but was smaller than that from Ectocarpales. The G + C content of D. divaricata (31.19%) was the highest of the known ptDNAs of brown algae (28.94-31.05%). Two protein-coding genes, rbcR and rpl32, were present in ptDNAs of Laminariales, Ectocarpales (Ectocarpus siliculosus), and Fucales (LEF) but were absent in D. divaricata. Reduced intergenic space (13.11%) and eight pairs of overlapping genes in D. divaricata ptDNA made it the most compact plastid genome in brown algae so far. The architecture of D. divaricata ptDNA showed higher similarity to that of Laminariales compared with Fucales and Ectocarpales. The difference in general features, gene content, and architecture among the ptDNAs of D. divaricata and LEF clade revealed the diversity and evolutionary trends of plastid genomes in brown algae.	[Liu, Feng; Jin, Zhe; Wang, Yu] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China; [Liu, Feng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; [Jin, Zhe] Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China; [Wang, Yu] Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China; [Bi, Yuping] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China; [Melton, James T., III] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA	Liu, F (corresponding author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China.; Liu, F (corresponding author), Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China.	liufeng@qdio.ac.cn			Key Research Program of Frontier Sciences, Chinese Academy of Sciences [QYZDB-SSW-DQC023]; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology [2016ASKJ02]; Key Research and Development Project of Shandong Province, China [2016GSF115041]; Strategic Priority Research Program, Chinese Academy of Sciences [XDA11020304]; Youth Innovation Promotion Association, Chinese Academy of Sciences [2015164]; Foundation for Huiquan Young Scholar of Institute of Oceanology, Chinese Academy of Sciences [2015]; Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, S.O.A. [MATHAB201701]; Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province of China [SDAIT-26-09]	This work was supported by the Key Research Program of Frontier Sciences, Chinese Academy of Sciences (No. QYZDB-SSW-DQC023), the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No. 2016ASKJ02), the Key Research and Development Project of Shandong Province, China (No. 2016GSF115041), the Strategic Priority Research Program, Chinese Academy of Sciences (No. XDA11020304), the Youth Innovation Promotion Association, Chinese Academy of Sciences (No. 2015164), the Foundation for Huiquan Young Scholar of Institute of Oceanology, Chinese Academy of Sciences (No. 2015), the Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, S.O.A. (No. MATHAB201701), and the Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province of China (No. SDAIT-26-09).	Abbas A, 2011, PAK J BOT, V43, P2207; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Baurain D, 2010, MOL BIOL EVOL, V27, P1698, DOI 10.1093/molbev/msq059; Benson G, 1999, NUCLEIC ACIDS RES, V27, P573, DOI 10.1093/nar/27.2.573; Brembu T, 2014, MAR GENOM, V16, P17, DOI 10.1016/j.margen.2013.12.002; Cattolico RA, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-211; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Cock JM, 2015, ADV MAR GENOMICS, V2, P335, DOI 10.1007/978-94-017-9642-2_16; Darling AE, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0011147; Darling ACE, 2004, GENOME RES, V14, P1394, DOI 10.1101/gr.2289704; Dorrell RG, 2017, ELIFE, V6, DOI 10.7554/eLife.23717; Hovde BT, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-604; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; Ji NY, 2009, MAR DRUGS, V7, P355, DOI 10.3390/md7030355; JONES DT, 1992, COMPUT APPL BIOSCI, V8, P275, DOI 10.1093/bioinformatics/8.3.275; Keeling PJ, 2010, PHILOS T R SOC B, V365, P729, DOI 10.1098/rstb.2009.0103; Keeling PJ, 2004, AM J BOT, V91, P1481, DOI 10.3732/ajb.91.10.1481; KIMURA M, 1980, J MOL EVOL, V16, P111, DOI 10.1007/BF01731581; KUHSEL M, 1985, PLANT MOL BIOL, V4, P365, DOI 10.1007/BF02418258; Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; Lillo F, 2002, BIOINFORMATICS, V18, P971, DOI 10.1093/bioinformatics/18.7.971; Liu F, 2016, J APPL PHYCOL, V28, P1419, DOI 10.1007/s10811-015-0609-2; Liu F, 2015, J APPL PHYCOL, V27, P1021, DOI 10.1007/s10811-014-0386-3; Lohse M, 2013, NUCLEIC ACIDS RES, V41, pW575, DOI 10.1093/nar/gkt289; Lozano-Orozco J. G., 2015, American Journal of Plant Sciences, V6, P2492, DOI 10.4236/ajps.2015.615251; Luo RB, 2012, GIGASCIENCE, V1, DOI 10.1186/2047-217X-1-18; Luthringer R, 2014, PERSPECT PHYCOL, V1, P11, DOI DOI 10.1127/2198-011X/2014/0002; Ong HC, 2010, J PHYCOL, V46, P602, DOI [10.1111/j.1529-8817.2010.00841x, 10.1111/j.1529-8817.2010.00841.x]; Oudot-Le Secq MP, 2007, MOL GENET GENOMICS, V277, P427, DOI 10.1007/s00438-006-0199-4; Rodriguez-Ezpeleta N, 2005, CURR BIOL, V15, P1325, DOI 10.1016/j.cub.2005.06.040; Ruck EC, 2014, GENOME BIOL EVOL, V6, P644, DOI 10.1093/gbe/evu039; Rumpho ME, 2008, P NATL ACAD SCI USA, V105, P17867, DOI 10.1073/pnas.0804968105; Sabir JSM, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0107854; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Schattner P, 2005, NUCLEIC ACIDS RES, V33, pW686, DOI 10.1093/nar/gki366; Sevcikova T, 2015, SCI REP-UK, V5, DOI 10.1038/srep10134; Silberfeld T, 2014, CRYPTOGAMIE ALGOL, V35, P117, DOI 10.7872/crya.v35.iss2.2014.117; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Starkenburg SR, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-212; Stoebe B, 1999, TRENDS GENET, V15, P344, DOI 10.1016/S0168-9525(99)01815-6; Tajima N, 2016, CURR GENET, V62, P887, DOI 10.1007/s00294-016-0598-y; Tamura K, 2004, P NATL ACAD SCI USA, V101, P11030, DOI 10.1073/pnas.0404206101; Tanaka T, 2011, PHOTOSYNTH RES, V109, P223, DOI 10.1007/s11120-011-9622-8; Terauchi M, 2017, MAR GENOM, V32, P49, DOI 10.1016/j.margen.2016.12.002; Thompson JD, 1997, NUCLEIC ACIDS RES, V25, P4876, DOI 10.1093/nar/25.24.4876; Tronholm A, 2010, J PHYCOL, V46, P1301, DOI 10.1111/j.1529-8817.2010.00908.x; Wang XL, 2013, MAR GENOM, V10, P1, DOI 10.1016/j.margen.2012.12.002; Wyman SK, 2004, BIOINFORMATICS, V20, P3252, DOI 10.1093/bioinformatics/bth352; Yurchenko T, 2016, OPEN BIOL, V6, DOI 10.1098/rsob.160249; Zhang L, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140144; Zhang L, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0139366; Zuckerkandl E., 1965, EVOLVING GENES PROTE, P97, DOI DOI 10.1016/B978-1-4832-2734-4.50017-6	53	18	18	1	15	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	1436-2228	1436-2236		MAR BIOTECHNOL	Mar. Biotechnol.	DEC	2017	19	6					627	637		10.1007/s10126-017-9781-5			11	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	FP5AN	WOS:000417629100008	29164355				2021-04-07	
J	Godfroy, O; Uji, T; Nagasato, C; Lipinska, AP; Scornet, D; Peters, AF; Avia, K; Colin, S; Mignerot, L; Motomura, T; Cock, JM; Coelho, SM				Godfroy, Olivier; Uji, Toshiki; Nagasato, Chikako; Lipinska, Agnieszka P.; Scornet, Delphine; Peters, Akira F.; Avia, Komlan; Colin, Sebastien; Mignerot, Laure; Motomura, Taizo; Cock, J. Mark; Coelho, Susana M.			DISTAG/TBCCd1 Is Required for Basal Cell Fate Determination in Ectocarpus	PLANT CELL			English	Article							BROWN ALGA ECTOCARPUS; PELVETIA-COMPRESSA ZYGOTES; BIASED GENE-EXPRESSION; LAND PLANTS; EARLY EMBRYOGENESIS; ARABIDOPSIS EMBRYO; FLOWERING PLANTS; GOLGI-APPARATUS; FUCUS ZYGOTES; ROOT HAIRS	Brown algae are one of the most developmentally complex groups within the eukaryotes. As in many land plants and animals, their main body axis is established early in development, when the initial cell gives rise to two daughter cells that have apical and basal identities, equivalent to shoot and root identities in land plants, respectively. We show here that mutations in the Ectocarpus DISTAG (DIS) gene lead to loss of basal structures during both the gametophyte and the sporophyte generations. Several abnormalities were observed in the germinating initial cell in dis mutants, including increased cell size, disorganization of the Golgi apparatus, disruption of the microtubule network, and aberrant positioning of the nucleus. DIS encodes a TBCCd1 protein, which has a role in internal cell organization in animals, Chlamydomonas reinhardtii, and trypanosomes. Our study highlights the key role of subcellular events within the germinating initial cell in the determination of apical/basal cell identities in a brown alga and emphasizes the remarkable functional conservation of TBCCd1 in regulating internal cell organization across extremely distant eukaryotic groups.	[Godfroy, Olivier; Uji, Toshiki; Lipinska, Agnieszka P.; Scornet, Delphine; Avia, Komlan; Mignerot, Laure; Cock, J. Mark; Coelho, Susana M.] UPMC Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models Stn Biol Roscoff, CNRS,Algal Genet Grp,UMR 8227, F-29688 Roscoff, France; [Nagasato, Chikako; Motomura, Taizo] Hokkaido Univ, Muroran Marine Stn, Sapporo, Hokkaido 0600808, Japan; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France; [Avia, Komlan] Univ Austral Chile, Pontificia Univ Catolica Chile, UMI Evolutionary Biol & Ecol Algae 3614, Sorbonne Univ,UPMC,CNRS,Stn Biol Roscoff, F-29688 Roscoff, France; [Colin, Sebastien] UPMC Univ Paris 06, Sorbonne Univ, CNRS, UMR7144,Stn Biol Roscoff, F-29680 Roscoff, France	Coelho, SM (corresponding author), UPMC Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models Stn Biol Roscoff, CNRS,Algal Genet Grp,UMR 8227, F-29688 Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020; Avia, Komlan/E-6850-2015	Avia, Komlan/0000-0001-6212-6774; Peters, Akira/0000-0001-5332-199X; Cock, J. Mark/0000-0002-2650-0383	CNRSCentre National de la Recherche Scientifique (CNRS)European Commission; Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [ANR-10-BLAN-1727, ANR-10-BTBR-04-01]; European Commission Interreg program France (Channel)-England (project Marinexus); UPMC; European CommissionEuropean CommissionEuropean Commission Joint Research Centre [638240]; Uehara Memorial FoundationUehara Memorial Foundation; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [17K07462] Funding Source: KAKEN	We thank Philippe Potin, Cecile Herve and Elizabeth Ficko-Blean for valuable discussions and the ABiMS Bioinformatics Core Service for providing access to Galaxy. This work was supported by the CNRS, the Agence Nationale de la Recherche (ANR-10-BLAN-1727 and ANR-10-BTBR-04-01), the European Commission Interreg program France (Channel)-England (project Marinexus), the UPMC, and the European Commission (grant agreement 638240). T.U. was supported by a fellowship from the Uehara Memorial Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Andre J, 2013, J CELL SCI, V126, P5350, DOI 10.1242/jcs.136515; Arun A, 2013, NEW PHYTOL, V197, P503, DOI 10.1111/nph.12007; Atkinson JA, 2014, PLANT PHYSIOL, V166, P538, DOI 10.1104/pp.114.245423; Avia K, 2017, SCI REP-UK, V7, DOI 10.1038/srep43241; Bartolini F, 2002, J BIOL CHEM, V277, P14629, DOI 10.1074/jbc.M200128200; BERGER F, 1994, SCIENCE, V263, P1421, DOI 10.1126/science.263.5152.1421; Bisgrove SR, 1998, DEV BIOL, V194, P246, DOI 10.1006/dbio.1997.8832; Bisgrove SR, 2001, PLANTA, V212, P648, DOI 10.1007/s004250000434; Bouget FY, 1998, DEVELOPMENT, V125, P1999; Brownlee C, 1998, SEMIN CELL DEV BIOL, V9, P179, DOI 10.1006/scdb.1997.0212; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2002, PLANT CELL, V14, P2369, DOI 10.1105/tpc.003285; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P369, DOI 10.1101/pdb.prot067975; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P262, DOI 10.1101/pdb.prot067942; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Conesa A., 2008, INT J PLANT GENOMICS, V2008; Copeland SJ, 2016, MOL BIOL CELL, V27, P260, DOI 10.1091/mbc.E15-02-0070; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Costa LM, 2014, SCIENCE, V344, P168, DOI 10.1126/science.1243005; Dolan L, 2009, CURR OPIN PLANT BIOL, V12, P4, DOI 10.1016/j.pbi.2008.12.001; Eme L., 2014, COLD SPRING HARB PER, DOI 101101/cshperspecta016139; Farnham G, 2013, J PHYCOL, V49, P819, DOI 10.1111/jpy.12096; Feldman JL, 2009, CURR BIOL, V19, P1238, DOI 10.1016/j.cub.2009.05.071; Fritsch F. E., 1935, STRUCTURE REPROD ALG; Godfroy O, 2015, MAR GENOM, V24, P109, DOI 10.1016/j.margen.2015.03.007; Goncalves J, 2010, EMBO REP, V11, P194, DOI 10.1038/embor.2010.5; Gonczy Pierre, 2005, WormBook, P1; GOODNER B, 1993, PLANT CELL, V5, P1471; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Haase G, 2015, FRONT NEUROSCI-SWITZ, V9, DOI 10.3389/fnins.2015.00448; Hable WE, 1998, DEV BIOL, V198, P45, DOI 10.1016/S0012-1606(98)80028-6; Hamann T, 1999, DEVELOPMENT, V126, P1387; Hardtke CS, 1998, EMBO J, V17, P1405, DOI 10.1093/emboj/17.5.1405; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Honkanen S, 2016, CURR BIOL, V26, P3238, DOI 10.1016/j.cub.2016.09.062; Horn T, 2010, NUCLEIC ACIDS RES, V38, pW332, DOI 10.1093/nar/gkq317; Jeong S, 2011, CURR BIOL, V21, P1268, DOI 10.1016/j.cub.2011.06.049; Jeong SH, 2011, J EXP BOT, V62, P1687, DOI 10.1093/jxb/erq398; Jones VAS, 2012, ANN BOT-LONDON, V110, P205, DOI 10.1093/aob/mcs136; Ketelaar T, 2002, PLANT CELL, V14, P2941, DOI 10.1105/tpc.005892; Kimata Y, 2016, P NATL ACAD SCI USA, V113, P14157, DOI 10.1073/pnas.1613979113; Koboldt Daniel C, 2013, Curr Protoc Bioinformatics, V44, DOI 10.1002/0471250953.bi1504s44; KROPF DL, 1988, SCIENCE, V239, P187, DOI 10.1126/science.3336780; Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]; Lau S, 2012, ANNU REV PLANT BIOL, V63, P483, DOI 10.1146/annurev-arplant-042811-105507; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Lipinska AP, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-909; Luthringer R, 2015, MOL BIOL EVOL, V32, P2973, DOI 10.1093/molbev/msv173; Macaisne N, 2017, DEVELOPMENT, V144, P409, DOI 10.1242/dev.141523; Maier I, 1997, EUR J PHYCOL, V32, P255; MITCHISON T, 1984, NATURE, V312, P237, DOI 10.1038/312237a0; Niklas KJ, 2010, NEW PHYTOL, V185, P27, DOI 10.1111/j.1469-8137.2009.03054.x; Nithianantham S, 2015, ELIFE, V4, DOI 10.7554/eLife.08811; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Pires ND, 2012, PHILOS T R SOC B, V367, P508, DOI 10.1098/rstb.2011.0252; Rademacher EH, 2012, DEV CELL, V22, P211, DOI 10.1016/j.devcel.2011.10.026; Rios RM, 2003, CURR OPIN CELL BIOL, V15, P60, DOI 10.1016/S0955-0674(02)00013-3; Rotmistrovsky K, 2004, NUCLEIC ACIDS RES, V32, pW108, DOI 10.1093/nar/gkh450; Sano R, 2005, EVOL DEV, V7, P69, DOI 10.1111/j.1525-142X.2005.05008.x; Scheffzek K, 1998, TRENDS BIOCHEM SCI, V23, P257, DOI 10.1016/S0968-0004(98)01224-9; Schmieder R, 2011, BIOINFORMATICS, V27, P863, DOI 10.1093/bioinformatics/btr026; Schneeberger K, 2009, NAT METHODS, V6, P550, DOI 10.1038/nmeth0809-550; Schneider H, 2013, ANNU PLANT REV, V45, P115, DOI 10.1002/9781118305881.ch4; Schwahn U, 1998, NAT GENET, V19, P327; Schwarz N, 2012, HUM MOL GENET, V21, P863, DOI 10.1093/hmg/ddr520; Shaw AJ, 2011, AM J BOT, V98, P352, DOI 10.3732/ajb.1000316; Shaw SL, 1996, DEVELOPMENT, V122, P2623; Song YY, 2015, TRENDS CELL BIOL, V25, P125, DOI 10.1016/j.tcb.2014.10.004; Steinborn K, 2002, GENE DEV, V16, P959, DOI 10.1101/gad.221702; Szovenyi P, 2011, MOL BIOL EVOL, V28, P803, DOI 10.1093/molbev/msq254; Tang DM, 2011, NAT COMMUN, V2, DOI 10.1038/ncomms1509; Tarver JE, 2015, NUCLEIC ACIDS RES, V43, P6384, DOI 10.1093/nar/gkv578; Tian GL, 1996, CELL, V86, P287, DOI 10.1016/S0092-8674(00)80100-2; Tian GL, 2013, METHOD CELL BIOL, V115, P155, DOI 10.1016/B978-0-12-407757-7.00011-6; Ueda M, 2012, CURR OPIN PLANT BIOL, V15, P578, DOI 10.1016/j.pbi.2012.08.001; Ueda M, 2011, DEV CELL, V20, P264, DOI 10.1016/j.devcel.2011.01.009; Vinogradova T, 2009, CELL CYCLE, V8, P2168, DOI 10.4161/cc.8.14.9074; Waki T, 2011, CURR BIOL, V21, P1277, DOI 10.1016/j.cub.2011.07.001; Whitaker D. M., 1931, BIOL BULL, V61, P249; Xie C, 2011, NUCLEIC ACIDS RES, V39, pW316, DOI 10.1093/nar/gkr483; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847; Zhong WM, 2011, CSH PERSPECT BIOL, V3, DOI 10.1101/cshperspect.a005363	86	6	6	0	10	AMER SOC PLANT BIOLOGISTS	ROCKVILLE	15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA	1040-4651	1532-298X		PLANT CELL	Plant Cell	DEC	2017	29	12					3102	3122		10.1105/tpc.17.00440			21	Biochemistry & Molecular Biology; Plant Sciences; Cell Biology	Biochemistry & Molecular Biology; Plant Sciences; Cell Biology	FS7UX	WOS:000422615500016	29208703	Bronze, Green Published			2021-04-07	
J	Teng, LH; Han, WT; Fan, X; Xu, D; Zhang, XW; Dittami, SM; Ye, NH				Teng, Linhong; Han, Wentao; Fan, Xiao; Xu, Dong; Zhang, Xiaowen; Dittami, Simon M.; Ye, Naihao			Evolution and Expansion of the Prokaryote-Like Lipoxygenase Family in the Brown Alga Saccharina japonica	FRONTIERS IN PLANT SCIENCE			English	Article						lipoxygenase; brown algae; gene duplication; selection pressure; functional divergence	ANTIOXIDANT ENZYME-ACTIVITIES; POSITIVE SELECTION; GENE FAMILY; CRYSTAL-STRUCTURE; CHEMICAL DEFENSE; GENOME; ACID; EXPRESSION; LAMINARIA; SEQUENCE	Lipoxygenase (LOX) plays important roles in fatty acid oxidation and lipid mediator biosynthesis. In this study, we give first insights into brown algal LOX evolution. Whole genome searches revealed four, three, and eleven LOXs in Ectocarpus siliculosus, Cladosiphon okamuranus, and Saccharina japonica, respectively. In phylogenetic analyses, LOXs from brown algae form a robust clade with those from prokaryotes, suggesting an ancestral origin and slow evolution. Brown algal LOXs were divided into two clades, C1 and C2 in a phylogenetic tree. Compared to the two species of Ectocarpales, LOX gene expansion occurred in the kelp S. japonica through tandem duplication and segmental duplication. Selection pressure analysis showed that LOX genes in brown algae have undergone strong purifying selection, while the selective constraint in the C2 clade was more relaxed than that in the C1 clade. Furthermore, within each clade, LOXs of S. japonica evolved under more relaxed selection constraints than E. siliculosus and C. okamuranus. Structural modeling showed that unlike LOXs of plants and animals, which contain a beta barrel in the N-terminal part of the protein, LOXs in brown algae fold into a single domain. Analysis of previously published transcriptomic data showed that LOXs in E. siliculosus are responsive to hyposaline, hypersaline, oxidative, and copper stresses. Moreover, clear divergence of expression patterns was observed among different life stages, as well as between duplicate gene pairs. In E. siliculosus, all four LOXs are male-biased in immature gametophytes, and mature gametophytes showed significantly higher LOX mRNA levels than immature gametophytes and sporophytes. In S. japonica, however, our RNA-Seq data showed that most LOXs are highly expressed in sporophytes. Even the most recently duplicated gene pairs showed divergent expression patterns, suggesting that functional divergence has likely occurred since LOX genes duplicated, which potentially contributes to the production of various oxylipins in brown algae.	[Teng, Linhong; Han, Wentao; Fan, Xiao; Xu, Dong; Zhang, Xiaowen; Ye, Naihao] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China; [Teng, Linhong; Ye, Naihao] Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China; [Dittami, Simon M.] CNRS, Stn Biol Roscoff, UMR 8227, Integrat Biol Marine Models, Roscoff, France; [Dittami, Simon M.] UPMC Univ Paris 06, Stn Biol Roscoff, Sorbonne Univ, UMR 8227,Integrat Biol Marine Models, Roscoff, France	Ye, NH (corresponding author), Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China.; Ye, NH (corresponding author), Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China.	yenh@ysfri.ac.cn			China Agriculture Research System [CARS-50]; Qingdao Municipal Science and Technology plan project [15-8-2-3-hy, 17-1-1-96-jch]; Taishan Scholars Funding; Central Public-interest Scientific Institution Basal Research Fund CAFS [2017HY-YJ01]; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences [20603022016001, 20603022016010]; AoShan Talents Program [2015ASTPES03]; Qingdao National Laboratory for Marine Science and Technology; Science Fund for Distinguished Young Scholars of Shandong Province [JQ201509]; National key Research and Development Plan [2016YFC1402102-2]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41676145]	This work was supported by China Agriculture Research System (CARS-50); Qingdao Municipal Science and Technology plan project (15-8-2-3-hy; 17-1-1-96-jch); Taishan Scholars Funding; Central Public-interest Scientific Institution Basal Research Fund CAFS (2017HY-YJ01); Special Scientific Research Funds for Central Non-Profit Institutes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (20603022016001, 20603022016010); AoShan Talents Program (No. 2015ASTPES03); Director fund Supported by Qingdao National Laboratory for Marine Science and Technology, the Science Fund for Distinguished Young Scholars of Shandong Province (JQ201509); National key Research and Development Plan (2016YFC1402102-2); National Natural Science Foundation of China (41676145). We are grateful to Agnieszka Lipinska for providing LOX expression profiles in different generations of E. siliculosus, and to the ABIMS platform (Roscoff) for providing access to their S. japonica genome browser.	Ackermann JA, 2017, BBA-MOL CELL BIOL L, V1862, P371, DOI 10.1016/j.bbalip.2016.07.014; Bailey TL, 2009, NUCLEIC ACIDS RES, V37, pW202, DOI 10.1093/nar/gkp335; Banthiya S, 2015, ARCH BIOCHEM BIOPHYS, V584, P116, DOI 10.1016/j.abb.2015.09.003; Barofsky A, 2007, ORG LETT, V9, P1017, DOI 10.1021/ol063051v; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; Biasini M, 2014, NUCLEIC ACIDS RES, V42, pW252, DOI 10.1093/nar/gku340; Blee E, 2002, TRENDS PLANT SCI, V7, P315, DOI 10.1016/S1360-1385(02)02290-2; Bouckaert R, 2014, PLOS COMPUT BIOL, V10, DOI 10.1371/journal.pcbi.1003537; Bueno P, 2001, NEW PHYTOL, V152, P91, DOI 10.1046/j.0028-646x.2001.00246.x; Chen YY, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0133809; Chen Z, 2016, FRONT GENET, V7, DOI 10.3339/fgene.2016.00176; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Crooks GE, 2004, GENOME RES, V14, P1188, DOI 10.1101/gr.849004; d'Ippolito G, 2009, NEW PHYTOL, V183, P1064, DOI 10.1111/j.1469-8137.2009.02887.x; Demuth JP, 2009, BIOESSAYS, V31, P29, DOI 10.1002/bies.080085; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dobson L, 2015, NUCLEIC ACIDS RES, V43, pW408, DOI 10.1093/nar/gkv451; Drummond DA, 2005, P NATL ACAD SCI USA, V102, P14338, DOI 10.1073/pnas.0504070102; Dupont CL, 2010, P NATL ACAD SCI USA, V107, P10567, DOI 10.1073/pnas.0912491107; Fatima N., 2017, PEERJ PREPRINTS, V5, DOI [10.7287/peerj.preprints.2906v1, DOI 10.7287/PEERJ.PREPRINTS.2906V1]; Feng B, 2010, J CEREAL SCI, V52, P387, DOI 10.1016/j.jcs.2010.06.019; Finn RD, 2016, NUCLEIC ACIDS RES, V44, pD279, DOI 10.1093/nar/gkv1344; Garreta A, 2013, FASEB J, V27, P4811, DOI 10.1096/fj.13-235952; Gerwick WH, 1999, ADV EXP MED BIOL, V447, P211; Gingerich DJ, 2007, PLANT CELL, V19, P2329, DOI 10.1105/tpc.107.051300; Good RT, 2014, GENOME BIOL EVOL, V6, P1118, DOI 10.1093/gbe/evu083; Gouet P, 2003, NUCLEIC ACIDS RES, V31, P3320, DOI 10.1093/nar/gkg556; Gruber A, 2015, PLANT J, V81, P519, DOI 10.1111/tpj.12734; Guo SL, 2017, ACTA PHYSIOL PLANT, V39, DOI 10.1007/s11738-017-2409-6; Hofmann M, 1997, PLANT CELL PHYSIOL, V38, P1046, DOI 10.1093/oxfordjournals.pcp.a029270; Holland PWH, 2017, PHILOS T R SOC B, V372, DOI 10.1098/rstb.2015.0480; Hou YL, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.01073; Hu B, 2015, BIOINFORMATICS, V31, P1296, DOI 10.1093/bioinformatics/btu817; Imbs AB, 2001, PHYTOCHEMISTRY, V58, P1067, DOI 10.1016/S0031-9422(01)00321-1; Jiang ZD, 2000, PHYTOCHEMISTRY, V53, P129, DOI 10.1016/S0031-9422(99)00445-8; Kalms J, 2017, BBA-MOL CELL BIOL L, V1862, P463, DOI 10.1016/j.bbalip.2017.01.003; Kawai H., 2016, EVOLUTION BIOGEOGRAP, DOI [10.1007/978-94-017-7534-2_9, DOI 10.1007/978-94-017-7534-2_9]; Koester JA, 2013, MOL BIOL EVOL, V30, P422, DOI 10.1093/molbev/mss242; Kosiol C, 2008, PLOS GENET, V4, DOI 10.1371/journal.pgen.1000144; Kousaka K, 2003, J NAT PROD, V66, P1318, DOI 10.1021/np030049t; Kupper FC, 2009, PLANT CELL PHYSIOL, V50, P789, DOI 10.1093/pcp/pcp023; Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]; Kumari P, 2014, MAR BIOTECHNOL, V16, P74, DOI 10.1007/s10126-013-9533-0; Lan T, 2009, PLANT CELL, V21, P3749, DOI 10.1105/tpc.109.070219; Li M, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-444; Li Y, 2016, ADV MATER TECHNOL-US, V1, DOI 10.1002/admt.201600102; Liavonchanka A, 2006, J PLANT PHYSIOL, V163, P348, DOI 10.1016/j.jplph.2005.11.006; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Liu HJ, 2015, MOL BIOL EVOL, V32, P2844, DOI 10.1093/molbev/msv156; Liu SQ, 2011, GENET MOL RES, V10, P2613, DOI 10.4238/2011.October.25.9; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; Lynch M, 2000, SCIENCE, V290, P1151, DOI 10.1126/science.290.5494.1151; Lynch M., 2007, ORIGINS GENOME ARCHI; MELAN MA, 1993, PLANT PHYSIOL, V101, P441, DOI 10.1104/pp.101.2.441; Minor W, 1996, BIOCHEMISTRY-US, V35, P10687, DOI 10.1021/bi960576u; Mock T, 2017, NATURE, V541, P536, DOI 10.1038/nature20803; Molina A, 2002, NEW PHYTOL, V156, P409, DOI 10.1046/j.1469-8137.2002.00527.x; Nishitsuji K, 2016, DNA RES, V23, P561, DOI 10.1093/dnares/dsw039; Ohno S., 1970, EVOLUTION GENE DUPLI; Oliver TA, 2010, GENOME BIOL EVOL, V2, P800, DOI 10.1093/gbe/evq063; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; Podolyan A, 2010, FUNCT PLANT BIOL, V37, P767, DOI 10.1071/FP09271; Pohnert G, 2002, NAT PROD REP, V19, P108, DOI 10.1039/a806888g; Pohnert G, 2002, PLANT PHYSIOL, V129, P103, DOI 10.1104/pp.010974; Porta H, 2001, MICROBIOL-SGM, V147, P3199, DOI 10.1099/00221287-147-12-3199; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Rensing SA, 2014, CURR OPIN PLANT BIOL, V17, P43, DOI 10.1016/j.pbi.2013.11.002; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Roux J, 2014, MOL BIOL EVOL, V31, P1661, DOI 10.1093/molbev/msu141; Shiu SH, 2006, P NATL ACAD SCI USA, V103, P2232, DOI 10.1073/pnas.0510388103; Sutliff RL, 2017, ACTA PHYSIOL, V219, P17, DOI 10.1111/apha.12753; Suyama M, 2006, NUCLEIC ACIDS RES, V34, pW609, DOI 10.1093/nar/gkl315; Tanaka KM, 2009, GENET RES, V91, P267, DOI 10.1017/S0016672309000196; Teng LH, 2017, FRONT PLANT SCI, V8, DOI 10.3389/fpls.2017.01429; Ulvskov P, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0076511; Weissenbach J, 2017, GENOME BIOL EVOL, V9, P241, DOI 10.1093/gbe/evw287; Wichard T, 2005, J CHEM ECOL, V31, P949, DOI 10.1007/s10886-005-3615-z; Xu D, 2017, GLOBAL CHANGE BIOL, V23, P4828, DOI 10.1111/gcb.13701; Yang ZH, 1998, MOL BIOL EVOL, V15, P1600, DOI 10.1093/oxfordjournals.molbev.a025888; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zambounis A, 2012, ALGAE-SEOUL, V27, P21, DOI 10.4490/algae.2012.27.1.021; Zhang C, 2014, SCI HORTIC-AMSTERDAM, V170, P94, DOI 10.1016/j.scienta.2014.03.005	84	10	10	0	12	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-462X			FRONT PLANT SCI	Front. Plant Sci.	NOV 28	2017	8								2018	10.3389/fpls.2017.02018			15	Plant Sciences	Plant Sciences	FN8XA	WOS:000416309800002	29234336	DOAJ Gold, Green Published			2021-04-07	
J	Muth, AF; Henriquez-Tejo, EA; Buschmann, AH				Muth, Arley F.; Henriquez-Tejo, Eduardo A.; Buschmann, Alejandro H.			Influence of sedimentation in the absence of macrograzers on recruitment of an annual population of Macrocystis pyrifera in Metri Bay, Chile	AUSTRAL ECOLOGY			English	Article						Macrocystis pyrifera; recruitment; sedimentation; substrate; turf algae	SOUTHERN CHILE; KELP FORESTS; PHAEOPHYTA; DEPOSITION; HETEROGENEITY; REPRODUCTION; CALIFORNIA; HABITAT; GROWTH; COAST	The effects of sedimentation and substrate orientation on algal and sessile invertebrate assemblages were tested on an annual population of Macrocystis pyrifera in Metri Bay, southern Chile. In the laboratory, M.pyrifera zoospores were seeded on Crepipatella fecunda shells, the primary substrate for M.pyrifera in this system. The seeded shells were deployed at Metri Bay inside cages and were orientated vertically and horizontally under two sedimentation regimes (bottom and suspended). Due to differences in grazer accessibility and the species present between the sedimentation treatments, grazers (>1cm) were excluded. We followed sporophyte development of M.pyrifera and the natural recruitment of other algal and invertebrate species. Sedimentation rates were significantly higher in the cages attached to the bottom compared to suspended cages (P<0.001). In total M.pyrifera and three additional algal genera were detected and all algal recruits showed significantly greater recruitment on the horizontally orientated substrate compared to the vertical substrate. Macrocystis pyrifera sporophytes were present only on the horizontal, suspended (less sedimentation) treatment. In contrast, Ulva and Ectocarpus spp. also occurred in the horizontal, high sediment treatment. Invertebrate recruitment (amphipods, barnacles and spirorbids) dominated the vertically oriented shells regardless of sedimentation. Results indicate that high sedimentation negatively affected the development of M.pyrifera sporophytes while other opportunistic species were able to recruit under these conditions.	[Muth, Arley F.] Moss Landing Marine Labs, Pob 450, Moss Landing, CA 95039 USA; [Henriquez-Tejo, Eduardo A.; Buschmann, Alejandro H.] Univ Lagos, Ctr I Mmar, Puerto Montt, Chile; [Henriquez-Tejo, Eduardo A.; Buschmann, Alejandro H.] Univ Lagos, CeBiB, Puerto Montt, Chile; [Muth, Arley F.] Univ Texas, Inst Marine Sci, 750 Channel View Dr, Port Aransas, TX 78373 USA	Muth, AF (corresponding author), Moss Landing Marine Labs, Pob 450, Moss Landing, CA 95039 USA.; Muth, AF (corresponding author), Univ Texas, Inst Marine Sci, 750 Channel View Dr, Port Aransas, TX 78373 USA.	amuth@mlml.calstate.edu	Buschmann, Alejandro H./B-4770-2012	Buschmann, Alejandro/0000-0003-3246-681X	Basal program of CONICYT-Chile [FB-0001]	We would like to dedicate this work to Victor Alonso Henriquez Soliz. Thank you to Adrian Villarroel, Robinson Altamirano and Sara Barrento for field help and Dr. Matthew Lee and Bianca Olivares at Centro i-mar for help with sediment analysis. We appreciate comments from two anonymous reviewers, Kyle Demes and Brynn Kaufmann on previous versions of this manuscript. This study was supported by a Basal program of CONICYT-Chile (FB-0001) to AHB.	Airoldi L, 2003, OCEANOGR MAR BIOL, V41, P161; Balata D, 2015, J EXP MAR BIOL ECOL, V467, P45, DOI 10.1016/j.jembe.2015.03.005; Buschmann A. H., 1992, COASTAL PLANT COMMUN, P91; Buschmann A. H, 2004, SEA URCHIN BIOL, P120; Buschmann AH, 2004, MAR BIOL, V145, P849, DOI 10.1007/s00227-004-1393-8; Buschmann AH, 2006, J APPL PHYCOL, V18, P575, DOI 10.1007/s10811-006-9063-5; Buschmann AH, 2014, J APPL PHYCOL, V26, P837, DOI 10.1007/s10811-013-0070-z; Chapman AS, 2002, J PHYCOL, V38, P894, DOI 10.1046/j.1529-8817.2002.t01-1-02025.x; Desmond MJ, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0123676; DEVINNY JS, 1978, MAR BIOL, V48, P343, DOI 10.1007/BF00391638; Diaz FJ, 2015, J MAR BIOL ASSOC UK, V95, P25, DOI 10.1017/S0025315414000228; Edwards MS, 2004, OECOLOGIA, V138, P436, DOI 10.1007/s00442-003-1452-8; Foster MS, 2010, J EXP MAR BIOL ECOL, V393, P59, DOI 10.1016/j.jembe.2010.07.002; Gonzalez HE, 2010, MAR ECOL PROG SER, V402, P13, DOI 10.3354/meps08360; Gorgula SK, 2004, MAR BIOL, V145, P613, DOI 10.1007/s00227-004-1335-5; Graham M. H., 2007, OCEANOGR MAR BIOL AN, V45, P3988; Graham MH, 1999, MAR BIOL, V135, P709, DOI 10.1007/s002270050672; Graham MH, 1997, MAR ECOL PROG SER, V148, P269, DOI 10.3354/meps148269; Henriquez LA, 2011, J PHYCOL, V47, P252, DOI 10.1111/j.1529-8817.2010.00955.x; Irving AD, 2002, MAR ECOL PROG SER, V245, P83, DOI 10.3354/meps245083; Ladah LB, 1999, J PHYCOL, V35, P1106, DOI 10.1046/j.1529-8817.1999.3561106.x; LEWIN R, 1986, SCIENCE, V234, P25, DOI 10.1126/science.234.4772.25; LITTLER MM, 1983, MAR ECOL PROG SER, V11, P129, DOI 10.3354/meps011129; Menge BA, 2001, MARINE COMMUNITY ECOLOGY, P221; Reed DC, 1996, ECOLOGY, V77, P300, DOI 10.2307/2265679; REED DC, 1991, J PHYCOL, V27, P361, DOI 10.1111/j.0022-3646.1991.00361.x; Rignot E, 2003, SCIENCE, V302, P434, DOI 10.1126/science.1087393; Rivera A, 2007, GLOBAL PLANET CHANGE, V59, P126, DOI 10.1016/j.gloplacha.2006.11.037; Roleda MY, 2011, MAR BIOL RES, V7, P213, DOI 10.1080/17451000.2010.497189; Sala E, 2002, P NATL ACAD SCI USA, V99, P3678, DOI 10.1073/pnas.052028499; Schiel DR, 2006, J EXP MAR BIOL ECOL, V331, P158, DOI 10.1016/j.jembe.2005.10.015; Underwood AJ, 2001, MARINE COMMUNITY ECOLOGY, P183; Underwood AJ., 1997, EXPT ECOLOGY THEIR L; Valle-Levinson A, 2007, ESTUAR COAST, V30, P113, DOI 10.1007/BF02782972; Witman JD, 2001, MARINE COMMUNITY ECOLOGY, P339	35	1	1	0	11	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	1442-9985	1442-9993		AUSTRAL ECOL	Austral Ecol.	NOV	2017	42	7					783	789		10.1111/aec.12496			7	Ecology	Environmental Sciences & Ecology	FK3RY	WOS:000413405000004					2021-04-07	
J	Teng, LH; Fan, X; Xu, D; Zhang, XW; Mock, T; Ye, NH				Teng, Linhong; Fan, Xiao; Xu, Dong; Zhang, Xiaowen; Mock, Thomas; Ye, Naihao			Identification of Genes under Positive Selection Reveals Differences in Evolutionary Adaptation between Brown-Algal Species	FRONTIERS IN PLANT SCIENCE			English	Article						brown algae; Saccharina japonica; Ectocarpus siliculosus; positive selection; adaptive evolution	FACILITATOR SUPERFAMILY TRANSPORTER; ECTOCARPUS-SILICULOSUS; RAPID EVOLUTION; PROTEIN; GENOME; TOOL; ARABIDOPSIS; COMPLEX; STRESS; TOLERANCE	Brown algae are an important taxonomic group in coastal ecosystems. The model brown algal species Ectocarpus siliculosus and Saccharina japonica are closely related lineages. Despite their close phylogenetic relationship, they vary greatly in morphology and physiology. To obtain further insights into the evolutionary forces driving divergence in brown algae, we analyzed 3,909 orthologs from both species to identify Genes Under Positive Selection (GUPS). About 12% of the orthologs in each species were considered to be under positive selection. Many GUPS are involved in membrane transport, regulation of homeostasis, and sexual reproduction in the small sporophyte of E. siliculosus, which is known to have a complex life cycle and to occupy a wide range of habitats. Genes involved in photosynthesis and cell division dominated the group of GUPS in the large kelp of S. japonica, which might explain why this alga has evolved the ability to grow very rapidly and to form some of the largest sporophytes. A significant number of molecular chaperones (e.g., heat-shock proteins) involved in stress responses were identified to be under positive selection in both species, potentially indicating their important roles for macroalgae to cope with the relatively variable environment of coastal ecosystems. Moreover, analysis of previously published microarray data of E. siliculosus showed that many GUPS in E. siliculosus were responsive to stress conditions, such as oxidative and hyposaline stress, whereas our RNA-seq data of S. japonica showed that GUPS in this species were most highly expressed in large sporophytes, which supports the suggestion that selection largely acts on different sets of genes in both marcoalgal species, potentially reflecting their adaptation to different ecological niches.	[Teng, Linhong; Fan, Xiao; Xu, Dong; Zhang, Xiaowen; Ye, Naihao] Chinese Acad Fishery, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China; [Mock, Thomas] Univ East Anglia, Sch Environm Sci, Norwich Res Pk, Norwich, Norfolk, England; [Ye, Naihao] Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China	Ye, NH (corresponding author), Chinese Acad Fishery, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China.; Ye, NH (corresponding author), Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China.	yenh@ysfri.ac.cn	Mock, Thomas/A-3127-2008	Mock, Thomas/0000-0001-9604-0362	earmarked fund for Modern Agro-industry Technology Research System [CARS-50]; Qingdao Municipal Science and Technology plan project [15-8-2-3-hy, 17-1-1-96-jch]; Central Public-interest Scientific Institution Basal Research Fund CAFS [2017HY-YJ01]; Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences [20603022016001, 20603022016010]; AoShan Talents Program [2015ASTPES03]; Director fund; Qingdao National Laboratory for Marine Science and Technology, the Science Fund for Distinguished Young Scholars of Shandong Province [JQ201509]; Program of Leading Talents of Qingdao [13-CX-27]; Talent Projects of Distinguished Scientific Scholars in Agriculture, Primary Research & Development Plan of Shandong Province [2015GSF115008]; National key Research & Development plan [2016YFC1402102-2]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41676145]; National Science & Technology Pillar Program [2013BAD23B01]; National Basic Research Special Foundation of China [2013FY110700]	This work was supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-50); Qingdao Municipal Science and Technology plan project (15-8-2-3-hy, 17-1-1-96-jch); Central Public-interest Scientific Institution Basal Research Fund CAFS (2017HY-YJ01); Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (20603022016001, 20603022016010); AoShan Talents Program (No. 2015ASTPES03); Director fund Supported by Qingdao National Laboratory for Marine Science and Technology, the Science Fund for Distinguished Young Scholars of Shandong Province (JQ201509); the Program of Leading Talents of Qingdao (13-CX-27); Talent Projects of Distinguished Scientific Scholars in Agriculture, Primary Research & Development Plan of Shandong Province (2015GSF115008); National key Research & Development plan (2016YFC1402102-2); National Natural Science Foundation of China (41676145); the National Science & Technology Pillar Program (2013BAD23B01) and National Basic Research Special Foundation of China (2013FY110700). We are grateful to Simon Dittami for help with microarray data processing.	ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Armbrust EV, 2001, APPL ENVIRON MICROB, V67, P3501, DOI 10.1128/AEM.67.8.3501-3513.2001; Ashburner M, 2000, NAT GENET, V25, P25, DOI 10.1038/75556; Bakewell MA, 2007, P NATL ACAD SCI USA, V104, P7489, DOI 10.1073/pnas.0701705104; Balakirev ES, 2012, BMC PLANT BIOL, V12, DOI 10.1186/1471-2229-12-108; Beech PL, 2000, SCIENCE, V287, P1276, DOI 10.1126/science.287.5456.1276; Biswas S, 2006, TRENDS GENET, V22, P437, DOI 10.1016/j.tig.2006.06.005; Blaby-Haas CE, 2012, BBA-MOL CELL RES, V1823, P1531, DOI 10.1016/j.bbamcr.2012.04.010; Bogumil D, 2012, BIOCHEMISTRY-US, V51, P9941, DOI 10.1021/bi3013643; Bulmer M. S., 2010, ENCY LIFE SCI, P49; Cagan A, 2016, MOL BIOL EVOL, V33, P3268, DOI 10.1093/molbev/msw215; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chen F, 2006, NUCLEIC ACIDS RES, V34, pD363, DOI 10.1093/nar/gkj123; Choe KN, 2017, MOL CELL, V65, P380, DOI 10.1016/j.molcel.2016.12.020; Cock JM, 2017, GENOME BIOL EVOL, V9, P740, DOI 10.1093/gbe/evx038; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Conesa A, 2005, BIOINFORMATICS, V21, P3674, DOI 10.1093/bioinformatics/bti610; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dorrell RG, 2017, ELIFE, V6, DOI 10.7554/eLife.23717; Drummond DA, 2005, P NATL ACAD SCI USA, V102, P14338, DOI 10.1073/pnas.0504070102; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Hayashi K, 2002, APPL ENVIRON MICROB, V68, P4996, DOI 10.1128/AEM.68.10.4996-5004.2002; Haydon MJ, 2007, PLANT PHYSIOL, V143, P1705, DOI 10.1104/pp.106.092015; Heinrich S, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0044342; Jensen JD, 2010, GENOME BIOL EVOL, V2, P371, DOI 10.1093/gbe/evq028; Kanehisa M, 2016, J MOL BIOL, V428, P726, DOI 10.1016/j.jmb.2015.11.006; Kawai H., 2016, SEAWEED PHYLOGEOGRAP; Kim YH, 2007, DEV BIOL, V302, P463, DOI 10.1016/j.ydbio.2006.10.004; Koester JA, 2013, MOL BIOL EVOL, V30, P422, DOI 10.1093/molbev/mss242; Kosiol C, 2008, PLOS GENET, V4, DOI 10.1371/journal.pgen.1000144; Kraft G., 1996, MUELLERIA, V9, P29; Letunic I, 2008, TRENDS BIOCHEM SCI, V33, P101, DOI 10.1016/j.tibs.2008.01.001; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Liu FL, 2014, J APPL PHYCOL, V26, P1585, DOI 10.1007/s10811-013-0188-z; Majorel C, 2014, ENV MICROBIOL REP, V6, P510, DOI 10.1111/1758-2229.12176; Manohar K, 2015, BMC MICROBIOL, V15, DOI 10.1186/s12866-015-0582-6; Margolin W, 2005, NAT REV MOL CELL BIO, V6, P862, DOI 10.1038/nrm1745; Mock T, 2017, NATURE, V541, P536, DOI 10.1038/nature20803; Nagao R, 2015, BIOCHEMISTRY-US, V54, P2022, DOI 10.1021/acs.biochem.5b00053; Nielsen R, 2005, PLOS BIOL, V3, P976, DOI 10.1371/journal.pbio.0030170; Niinemets U, 2017, NEW PHYTOL, V213, P43, DOI 10.1111/nph.14307; Oliver TA, 2010, GENOME BIOL EVOL, V2, P800, DOI 10.1093/gbe/evq063; Petersen J, 2014, GENOME BIOL EVOL, V6, P666, DOI 10.1093/gbe/evu043; Puthiyaveetil S, 2013, FRONT PLANT SCI, V4, DOI 10.3389/fpls.2013.00459; R Development Core Team, 2014, R LANG ENV STAT COMP; Reddy VS, 2012, FEBS J, V279, P2022, DOI 10.1111/j.1742-4658.2012.08588.x; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Roux J, 2014, MOL BIOL EVOL, V31, P1661, DOI 10.1093/molbev/msu141; Saint-Marcoux D, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00054; Suyama M, 2006, NUCLEIC ACIDS RES, V34, pW609, DOI 10.1093/nar/gkl315; Swift DG, 2016, BMC EVOL BIOL, V16, DOI 10.1186/s12862-016-0696-y; Tesson B, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00471; Warnes G, 2016, GPLOTS VARIOUS R PRO; Weele C. V. D., 2007, MOL BIOL CELL, V18, P3711, DOI [10.1091/mbc.E06-11-0979, DOI 10.1091/MBC.E06-11-0979]; Xie C, 2011, NUCLEIC ACIDS RES, V39, pW316, DOI 10.1093/nar/gkr483; Xu RQ, 2004, GENE, V324, P35, DOI 10.1016/j.gene.2003.09.025; Xu RQ, 2006, PLANT MOL BIOL, V61, P799, DOI 10.1007/s11103-006-0051-6; Xu XX, 2014, FUNGAL GENET BIOL, V69, P75, DOI 10.1016/j.fgb.2014.06.002; Yamada T, 2011, NUCLEIC ACIDS RES, V39, pW412, DOI 10.1093/nar/gkr313; Yang ZH, 2005, MOL BIOL EVOL, V22, P1107, DOI 10.1093/molbev/msi097; Yang ZH, 1998, MOL BIOL EVOL, V15, P1600, DOI 10.1093/oxfordjournals.molbev.a025888; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Yang ZH, 2011, MOL BIOL EVOL, V28, P1217, DOI 10.1093/molbev/msq303; Ye J, 2006, NUCLEIC ACIDS RES, V34, pW293, DOI 10.1093/nar/gkl031; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986	65	9	9	0	17	FRONTIERS MEDIA SA	LAUSANNE	PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND	1664-462X			FRONT PLANT SCI	Front. Plant Sci.	AUG 15	2017	8								1429	10.3389/fpls.2017.01429			11	Plant Sciences	Plant Sciences	FD7MD	WOS:000407709300001	28861104	DOAJ Gold, Green Published, Green Accepted			2021-04-07	
J	Murua, P; Goecke, F; Westermeier, R; van West, P; Kupper, FC; Neuhauser, S				Murua, Pedro; Goecke, Franz; Westermeier, Renato; van West, Pieter; Kupper, Frithjof C.; Neuhauser, Sigrid			Maullinia braseltonii sp nov (Rhizaria, Phytomyxea, Phagomyxida): A Cyst-forming Parasite of the Bull Kelp Durvillaea spp. (Stramenopila, Phaeophyceae, Fucales)	PROTIST			English	Article						plasmodiophorids; brown algae; galls; rDNA; resting spores	PLASMODIOPHORA-BRASSICAE; ECTOCARPUS-SILICULOSUS; PHYLOGENETIC ANALYSIS; EURYCHASMA-DICKSONII; ANTARCTICA; CHILE; ALGAE; SEQUENCE; DURVILLEALES; CULTIVATION	Phytomyxea are obligate endoparasites of angiosperm plants and Stramenopiles characterised by a complex life cycle. Here Maullinia braseltonii sp. nov., an obligate parasite infecting the bull kelp Durvillaea (Phaeophyceae, Fucales) from the South-Eastern Pacific (Central Chile and Chiloe Island) and South-Western Atlantic (Falkland Islands, UK) is described. M. braseltonii causes distinct hypertrophies (galls) on the host thalli making it easily identifiable in the field. Sequence comparisons based on the partial 18S and the partial 18S-5.8S-28S regions confirmed its placement within the order Phagomyxida (Phytomyxea, Rhizaria), as a sister species of the marine parasite Maullinia ectocarpii, which is also a parasite of brown algae. The development of resting spores in M. braseltonii is described by light and electron microscopy and confirmed by FISH experiments, which visually showed the differential expression of the 28S non-coding gene, strongly in early plasmodia and weakly in late cysts. M. braseltonii is, so far, the only phytomyxean parasite of brown algae for which the formation of resting spores has been reported, and which is widely distributed in Durvillaea stocks from the Southeastern Pacific and (C) 2017 The Authors. Published by Elsevier GmbH.	[Murua, Pedro; Kupper, Frithjof C.] Univ Aberdeen, Oceanlab Sch Biol Sci, Main St, Newburgh AB41 6AA, England; [Murua, Pedro; van West, Pieter] Univ Aberdeen, Int Ctr Aquaculture Res & Dev, Aberdeen Oomycete Lab, Foresterhill, Aberdeen AB25 2ZD, Scotland; [Murua, Pedro] Scottish Assoc Marine Sci, Scottish Marine Inst Culture Collect Algae & Prot, Oban PA37 1QA, Argyll, Scotland; [Goecke, Franz] Norwegian Univ Life Sci NMBU, Dept Plant & Environm Sci IPV, As, Norway; [Westermeier, Renato] Univ Austral Chile, Inst Acuicultura, Lab Macroalgas, POB 1327, Puerto Montt, Chile; [Neuhauser, Sigrid] Univ Innsbruck, lnst Microbiol, Innsbruck, Tyrol, Austria	Neuhauser, S (corresponding author), Univ Innsbruck, lnst Microbiol, Innsbruck, Tyrol, Austria.	sigrid.neuhauser@uibk.ac.a	Murua, Pedro/J-6397-2014; Murua, Pedro/L-6686-2019; Neuhauser, Sigrid/U-1603-2019; Neuhauser, Sigrid/C-8189-2014	Murua, Pedro/0000-0002-1598-7261; Murua, Pedro/0000-0002-1598-7261; Neuhauser, Sigrid/0000-0003-0305-1615; Neuhauser, Sigrid/0000-0003-0305-1615; Kuepper, Frithjof/0000-0003-1273-7109; van West, Pieter/0000-0002-0767-6017	Conicyt (BecasChile)Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) [72130422]; NERC IOF Pump-priming [NE/L013223/1]; Gobierno Regional de Los Lagos [FIC 2012 E7259-2, FIC 2013 BIP30234872-0]; Austrian Science Fund (FWF)Austrian Science Fund (FWF) [J3175-B20, Y801-B16]; UoA; BBSRCUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC); NERCUK Research & Innovation (UKRI)NERC Natural Environment Research Council; Scottish Funding Council [HR09011]; Shackleton Fund (FCK); John Cheek Fund (FCK); Austrian Science Fund (FWF)Austrian Science Fund (FWF) [J 3175, Y 801] Funding Source: researchfish; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/P020224/1, BB/M026566/1] Funding Source: researchfish; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [1093492] Funding Source: researchfish	Help in biomass collection by David J. Patino (UACh), Liliana A. Munoz (University of Aberdeen (UoA)) and Alexandra Mystikou (South Atlantic Environmental Research Institute & UoA), and in conducting electron microscopy by Gillian Milne (Aberdeen Microscopy Facility) is acknowledged. Thanks are due to the three anonymous reviewers, whose comments helped to improve the earlier version of this manuscript. PM was funded by Conicyt (BecasChile No 72130422) for PhD studies at the University of Aberdeen, and by the NERC IOF Pump-priming (scheme NE/L013223/1) for activities at the Scottish Association for Marine Sciences. RW thanks financial support from Gobierno Regional de Los Lagos (projects FIC 2012 E7259-2 and FIC 2013 BIP30234872-0) and Fondef, Conicyt (HUAM AQ12I0010), which allows the sampling expeditions at Chiloe Island by David J. Patino, Liliana Munoz and PM. SN was funded by the Austrian Science Fund (FWF): grant J3175-B20 (Erwin Schrodinger Fellowship) and grant Y801-B16 (START-grant). PvW is supported by the UoA, BBSRC and NERC. Also, the MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) is gratefully acknowledged for its support to FCK. Finally, we would like to thank the UoA, Shackleton Fund (FCK) and the John Cheek Fund (FCK) for supporting the expeditions of Alexandra Mystikou, PvW and FCK to the Falkland Islands.	Aguilera M, 1988, GAYANA BOT, V45, P337; Bulman S., 2014, SYSTEMATICS EVOLUT A, P99; Bulman SR, 2001, PROTIST, V152, P43, DOI 10.1078/1434-4610-00042; Castilla JC, 2007, ECOL APPL, V17, P1511, DOI 10.1890/06-1285.1; CASTILLA JC, 1989, MAR ECOL PROG SER, V50, P203, DOI 10.3354/meps050203; Dillehay TD, 2008, SCIENCE, V320, P784, DOI 10.1126/science.1156533; Dixon GR, 2009, J PLANT GROWTH REGUL, V28, P194, DOI 10.1007/s00344-009-9090-y; Fraser CI, 2011, P ROY SOC B-BIOL SCI, V278, P649, DOI 10.1098/rspb.2010.1117; Fraser CI, 2010, MOL PHYLOGENET EVOL, V57, P1301, DOI 10.1016/j.ympev.2010.10.011; Fraser CI, 2010, BMC EVOL BIOL, V10, DOI 10.1186/1471-2148-10-203; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Goecke F, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0045358; Goecke F, 2010, MAR ECOL PROG SER, V409, P267, DOI 10.3354/meps08607; Graiff A, 2013, BOT MAR, V56, P3, DOI 10.1515/bot-2012-0193; Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010; Guiry MD, 2016, DURVILLAEA WWW DOCUM; Jahnke R, 1978, STUDY GALL DIS LAMIN; Kanyuka K, 2003, MOL PLANT PATHOL, V4, P393, DOI 10.1046/J.1364-3703.2003.00177.X; Katoh K, 2013, MOL BIOL EVOL, V30, P772, DOI 10.1093/molbev/mst010; Kearse M, 2012, BIOINFORMATICS, V28, P1647, DOI 10.1093/bioinformatics/bts199; Lopez BA, 2017, J PHYCOL, V53, P70, DOI 10.1111/jpy.12479; Loureiro R, 2015, NEW PHYTOL, V206, P489, DOI 10.1111/nph.13278; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; MILLER CHARLES E., 1958, JOUR ELISHA MITCHELL SCI SOC, V74, P49; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; Neuhauser S, 2014, BMC EVOL BIOL, V14, DOI 10.1186/1471-2148-14-33; Neuhauser S, 2011, MAR FRESHWATER RES, V62, P365, DOI 10.1071/MF10282; Parodi ER, 2010, BIOCELL, V34, P45; Ricker RW, 1987, TAXONOMY BIOGEOGRAPH; Ronquist F, 2012, SYST BIOL, V61, P539, DOI 10.1093/sysbio/sys029; Santala J, 2010, ANN APPL BIOL, V157, P163, DOI 10.1111/j.1744-7348.2010.00423.x; Sawabe T, 1998, INT J SYST BACTERIOL, V48, P769, DOI 10.1099/00207713-48-3-769; SCHIEL DR, 1990, HYDROBIOLOGIA, V204, P25, DOI 10.1007/BF00040211; Schnepf E, 2000, HELGOLAND MAR RES, V54, P237, DOI 10.1007/s101520000056; Schroeder DC, 2015, PERSPECT PHYCOL, V2, P105, DOI DOI 10.1127/PIP/2015/0034; Schwelm A, 2016, PROTIST, V167, P544, DOI 10.1016/j.protis.2016.08.008; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Sernapesca, 2015, ANN EST PESC 2014; SOUTH G R, 1974, Journal of the Royal Society of New Zealand, V4, P455; Stamatakis A, 2014, BIOINFORMATICS, V30, P1312, DOI 10.1093/bioinformatics/btu033; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Taylor DI, 2005, MAR ECOL PROG SER, V288, P87, DOI 10.3354/meps288087; WESTERMEIER R, 1994, MAR ECOL PROG SER, V110, P187, DOI 10.3354/meps110187; WESTERMEIER R, 1991, REV CHIL HIST NAT, V64, P307	45	16	16	0	9	ELSEVIER GMBH, URBAN & FISCHER VERLAG	JENA	OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY	1434-4610			PROTIST	Protist	AUG	2017	168	4					468	480		10.1016/j.protis.2017.07.001			13	Microbiology	Microbiology	FG3HB	WOS:000410031300008	28822911	Green Published, Green Accepted, Other Gold			2021-04-07	
J	Yeo, KB; Ki, MR; Park, KS; Pack, SP				Yeo, Ki Baek; Ki, Mi-Ran; Park, Ki Sung; Pack, Seung Pil			Novel silica-forming peptides derived from Ectocarpus siliculosus	PROCESS BIOCHEMISTRY			English	Article						Biosilica; Silica-forming peptide; Ectocarpus siliculosus; Organic-inorganic complex	CYLINDROTHECA-FUSIFORMIS; BIOSILICA; IMMOBILIZATION; DIATOMS; MORPHOGENESIS; SILAFFINS; PROTEIN	A synthetic R5 peptide showed the silica precipitation activity when added to silicic acid solution under ambient conditions. However, R5 peptide showed no silica precipitation activity below pH 7, an important pH range for biological applications. Here, we reported new silica-forming peptides (SFP), named EctP1 and EctP2. They showed better silica deposition ability than the R5 peptide. Particularly, at pH 6, EctP1 showed silica deposition activity while R5 did not. EctP1, EctP2, and R5 were genetically fused to the C-terminus of green fluorescent protein (GFP). GFP-SFP fusion proteins showed silicification ability, and GFP-EctP1 fusion protein showed the capacious silicification activity at wide pH range. The GFP-EctP2 fusion proteins showed higher protein expression levels than other fusion proteins. Furthermore, silicified GFP-SFP fusion protein exhibited an organic inorganic complex form. These results indicate that the SFP fusion system is a novel tool for immobilizing biomolecules on silica material for biological and industrial applications.	[Yeo, Ki Baek; Ki, Mi-Ran; Park, Ki Sung; Pack, Seung Pil] Korea Univ, Dept Biotechnol & Bioinformat, Sejong Ro 2511, Sejong 30019, South Korea	Pack, SP (corresponding author), Korea Univ, Dept Biotechnol & Bioinformat, Sejong Ro 2511, Sejong 30019, South Korea.	spack@korea.ac.kr			Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning, Korea [NRF-2015M1A5A1037054]; Marine Biomaterials Research Center Grant from Marine Biotechnology Program - Ministry of Oceans and Fisheries, Korea	This work was supported by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning, Korea ( < gn1 > NRF-2015M1A5A1037054 < / gn1) and by the Marine Biomaterials Research Center Grant from Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries, Korea.	He QJ, 2011, J MATER CHEM, V21, P5845, DOI 10.1039/c0jm03851b; Hoffmann F, 2006, ANGEW CHEM INT EDIT, V45, P3216, DOI 10.1002/anie.200503075; Ki MR, 2014, PROCESS BIOCHEM, V49, P95, DOI 10.1016/j.procbio.2013.10.010; Ki MR, 2013, BIOPROC BIOSYST ENG, V36, P643, DOI 10.1007/s00449-012-0818-x; Knecht MR, 2003, CHEM COMMUN, P3038, DOI 10.1039/b309074d; Kroger N, 1999, SCIENCE, V286, P1129, DOI 10.1126/science.286.5442.1129; Kroger N, 2002, SCIENCE, V298, P584, DOI 10.1126/science.1076221; Kroger N, 2001, J BIOL CHEM, V276, P26066, DOI 10.1074/jbc.M102093200; Kropp D., 2009, ANAL CHIM ACTA, V647, P14; Lechner CC, 2015, MAR DRUGS, V13, P5297, DOI 10.3390/md13085297; Luckarift HR, 2004, NAT BIOTECHNOL, V22, P211, DOI 10.1038/nbt931; Martin-Jezequel V, 2000, J PHYCOL, V36, P821, DOI 10.1046/j.1529-8817.2000.00019.x; Morse DE, 1999, TRENDS BIOTECHNOL, V17, P230, DOI 10.1016/S0167-7799(99)01309-8; Muller WEG, 2005, CELL TISSUE RES, V321, P285, DOI 10.1007/s00441-005-1141-5; Nam DH, 2009, BIOTECHNOL PROGR, V25, P1643, DOI 10.1002/btpr.261; Poulsen N, 2003, P NATL ACAD SCI USA, V100, P12075, DOI 10.1073/pnas.2035131100; Senior L, 2015, J MATER CHEM B, V3, P2607, DOI 10.1039/c4tb01679c; Wang CJ, 2015, NANOSCALE, V7, P2527, DOI 10.1039/c4nr06232a; White SH, 1998, BBA-REV BIOMEMBRANES, V1376, P339, DOI 10.1016/S0304-4157(98)00021-5; Xi M.R., 2016, PROCESS BIOCHEM, V51, P1222	20	11	12	1	15	ELSEVIER SCI LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND	1359-5113	1873-3298		PROCESS BIOCHEM	Process Biochem.	JUL	2017	58						193	198		10.1016/j.procbio.2017.04.004			6	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering	EZ4VN	WOS:000404710700025					2021-04-07	
J	Montecinos, AE; Guillemin, ML; Couceiro, L; Peters, AF; Stoeckel, S; Valero, M				Montecinos, Alejandro E.; Guillemin, Marie-Laure; Couceiro, Lucia; Peters, Akira F.; Stoeckel, Solenn; Valero, Myriam			Hybridization between two cryptic filamentous brown seaweeds along the shore: analysing pre- and postzygotic barriers in populations of individuals with varying ploidy levels	MOLECULAR ECOLOGY			English	Article						contact zone; Ectocarpus; haploid-diploid life cycle; meiosis; reproductive barrier; shore gradient	ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; ALGA ECTOCARPUS; LIFE-CYCLES; HYBRID ZONES; MICROSATELLITE MARKERS; REPRODUCTIVE ISOLATION; INTERSPECIFIC HYBRIDS; CHROMOSOMAL EVOLUTION; VESICULOSUS FUCACEAE; NATURAL-POPULATIONS	We aimed to study the importance of hybridization between two cryptic species of the genus Ectocarpus, a group of filamentous algae with haploid-diploid life cycles that include the principal genetic model organism for the brown algae. In haploid-diploid species, the genetic structure of the two phases of the life cycle can be analysed separately in natural populations. Such life cycles provide a unique opportunity to estimate the frequency of hybrid genotypes in diploid sporophytes and meiotic recombinant genotypes in haploid gametophytes allowing the effects of reproductive barriers preventing fertilization or preventing meiosis to be untangle. The level of hybridization between E. siliculosus and E. crouaniorum was quantified along the European coast. Clonal cultures (568 diploid, 336 haploid) isolated from field samples were genotyped using cytoplasmic and nuclear markers to estimate the frequency of hybrid genotypes in diploids and recombinant haploids. We identified admixed individuals using microsatellite loci, classical assignment methods and a newly developed Bayesian method (XPloidAssignment), which allows the analysis of populations that exhibit variations in ploidy level. Over all populations, the level of hybridization was estimated at 8.7%. Hybrids were exclusively observed in sympatric populations. More than 98% of hybrids were diploids (40% of which showed signs of aneuploidy) with a high frequency of rare alleles. The near absence of haploid recombinant hybrids demonstrates that the reproductive barriers are mostly postzygotic and suggests that abnormal chromosome segregation during meiosis following hybridization of species with different genome sizes could be a major cause of interspecific incompatibility in this system.	[Montecinos, Alejandro E.; Guillemin, Marie-Laure; Couceiro, Lucia; Valero, Myriam] Sorbonne Univ, UMI EBEA Evolutionary Biol & Ecol Algae 3614, Stn Biol Roscoff, CNRS,UPMC,PUC,UACH, CS 90074,Pl Georges Teissier, F-29688 Roscoff, France; [Montecinos, Alejandro E.; Guillemin, Marie-Laure] Univ Austral Chile, Inst Ciencias Ambientales & Evolutivas, Fac Ciencias, Casilla 567, Valdivia, Chile; [Peters, Akira F.] Bezhin Rosko, 40 Rue Pecheurs, F-29250 Santec, France; [Stoeckel, Solenn] Univ Rennes 1, INRA, Agrocampus Ouest, IGEPP, Rennes, France; [Couceiro, Lucia] Univ A Coruna, Dept Anim Biol Plant Biol & Ecol, La Coruna, Spain	Valero, M (corresponding author), Sorbonne Univ, UMI EBEA Evolutionary Biol & Ecol Algae 3614, Stn Biol Roscoff, CNRS,UPMC,PUC,UACH, CS 90074,Pl Georges Teissier, F-29688 Roscoff, France.	valero@sb-roscoff.fr	Couceiro, Lucia/M-1851-2014; Stoeckel, Solenn/K-8086-2017; Valero, Myriam/M-6052-2019	Couceiro, Lucia/0000-0003-4300-5744; Stoeckel, Solenn/0000-0001-6064-5941; Valero, Myriam/0000-0002-9000-1423; Peters, Akira/0000-0001-5332-199X; Couceiro, Lucia/0000-0001-9466-8164	Becas-Chile, CONICYT; project MARINEXUS (EU INTERREG programme France (Channel)-UK); project Bi-Cycle (France) [ANR10-BLAN-1727]; project Clonix (France) [ANR11-BSV7-00704]; project IDEALG (France) [ANR-10-BTBR-04]; project 'Bibliotheque du vivant' (France: INRA-MNHN-INEE-CNRS); international research network 'Diversity, Evolution and Biotechnology of Marine Algae' (GDRI) [0803]	We would like to thank Chloe Jollivet, Laurence Dartevelle and Jerome Coudret for help in maintaining strains in the Station Biologique de Roscoff. We also thank Stephane Mauger and Murray Brown for assistance in the molecular laboratory and the field, respectively. Declan Schroeder (MBA, Plymouth) and Ignacio Barbara (Universidade da Coruna) kindly welcomed us for isolation work in their laboratories. We particularly appreciated insightful and constructive comments from Thomas Broquet, Christophe Destombe, Mark Cock and two anonymous reviewers. Special thanks to Christophe Destombe who conceived and drew Figure 1. Principal funding came from a doctoral grant to Alejandro Montecinos (Becas-Chile, CONICYT, advanced human resources program), additional support from the projects MARINEXUS (EU INTERREG programme France (Channel)-UK), Bi-Cycle (France: ANR10-BLAN-1727), Clonix (France: ANR11-BSV7-00704), IDEALG (France: ANR-10-BTBR-04), 'Bibliotheque du vivant' (France: INRA-MNHN-INEE-CNRS) and from the international research network 'Diversity, Evolution and Biotechnology of Marine Algae' (GDRI No. 0803).	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Anderson LE, 1982, J HATTORI BOT LAB, V52, P241; Arnaud-Haond S, 2007, MOL ECOL NOTES, V7, P15, DOI 10.1111/j.1471-8286.2006.01522.x; ARNHEIM N, 1980, P NATL ACAD SCI-BIOL, V77, P7323, DOI 10.1073/pnas.77.12.7323; BARTON NH, 1985, ANNU REV ECOL SYST, V16, P113, DOI 10.1146/annurev.es.16.110185.000553; Belkhir K., 2004, 5171 CNRS UMR; Bergstrom L, 2005, J PHYCOL, V41, P1025, DOI 10.1111/j.1529-8817.2005.00125.x; Bierne N, 2003, MOL ECOL, V12, P447, DOI 10.1046/j.1365-294X.2003.01730.x; Billard E, 2005, EUR J PHYCOL, V40, P397, DOI 10.1080/09670260500334354; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; Coyer JA, 2007, J EVOLUTION BIOL, V20, P2322, DOI 10.1111/j.1420-9101.2007.01411.x; Coyer JA, 2011, MOL PHYLOGENET EVOL, V58, P283, DOI 10.1016/j.ympev.2010.11.015; Coyer JA, 2001, J PHYCOL, V37, P574, DOI 10.1046/j.1529-8817.2001.037001574.x; Coyer JA, 2006, MOL PHYLOGENET EVOL, V39, P209, DOI 10.1016/j.ympev.2006.01.019; Coyer JA, 2002, P ROY SOC B-BIOL SCI, V269, P1829, DOI 10.1098/rspb.2002.2093; Coyne J.A., 2004, SPECIATION; Destombe C, 2010, J PHYCOL, V46, P720, DOI 10.1111/j.1529-8817.2010.00846.x; Dorken ME, 2001, J ECOL, V89, P339, DOI 10.1046/j.1365-2745.2001.00558.x; DOVER G, 1994, CURR BIOL, V4, P1165, DOI 10.1016/S0960-9822(00)00265-7; Engel CR, 2005, MOL ECOL, V14, P2033, DOI 10.1111/j.1365-294X.2005.02558.x; Evanno G, 2005, MOL ECOL, V14, P2611, DOI 10.1111/j.1365-294X.2005.02553.x; Fama P, 2000, EUR J PHYCOL, V35, P349, DOI 10.1017/S0967026200002948; Geoffroy A, 2015, J PHYCOL, V51, P480, DOI 10.1111/jpy.12291; Giraud T, 2008, FUNGAL GENET BIOL, V45, P791, DOI 10.1016/j.fgb.2008.02.001; Guillemin ML, 2016, J PHYCOL, V52, P806, DOI 10.1111/jpy.12440; Hoarau G, 2015, ROY SOC OPEN SCI, V2, DOI 10.1098/rsos.140538; Ito H, 2015, SCI REP-UK, V5, DOI 10.1038/srep13171; Jakobsson M, 2007, BIOINFORMATICS, V23, P1801, DOI 10.1093/bioinformatics/btm233; Johannesson K, 2009, EVOL ECOL, V23, P5, DOI 10.1007/s10682-007-9225-1; Jombart T, 2008, BIOINFORMATICS, V24, P1403, DOI 10.1093/bioinformatics/btn129; KNIGHT MARGERY, 1929, TRANS ROY SOC EDINBURGH, V56, P307; Kohn LM, 2005, ANNU REV PHYTOPATHOL, V43, P279, DOI 10.1146/annurev.phyto.43.040204.135958; Lammers Y, 2013, MOL ECOL RESOUR, V13, P237, DOI 10.1111/1755-0998.12044; Lavretsky P, 2016, MOL ECOL, V25, P661, DOI 10.1111/mec.13487; Lexer C, 2007, HEREDITY, V98, P74, DOI 10.1038/sj.hdy.6800898; Liao PC, 2010, BOT STUD, V51, P413; Liti G, 2006, GENETICS, V174, P839, DOI 10.1534/genetics.106.062166; Livingstone K, 2004, NEW PHYTOL, V161, P107, DOI 10.1046/j.1469-8137.2003.00942.x; Mable BK, 1998, BIOESSAYS, V20, P453, DOI 10.1002/(SICI)1521-1878(199806)20:6<453::AID-BIES3>3.0.CO;2-N; Maggs CA, 2011, INTEGR COMP BIOL, V51, P492, DOI 10.1093/icb/icr075; Montecinos AE, 2017, J PHYCOL, V53, P17, DOI 10.1111/jpy.12452; Monteiro CA, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0035978; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; MULLER DG, 1980, NATURWISSENSCHAFTEN, V67, P462, DOI 10.1007/BF00405647; Neiva J, 2010, MOL ECOL, V19, P4812, DOI 10.1111/j.1365-294X.2010.04853.x; Niwa K, 2014, J PHYCOL, V50, P897, DOI 10.1111/jpy.12220; Paetkau D, 2004, MOL ECOL, V13, P55, DOI 10.1046/j.1365-294X.2004.02008.x; Peakall R, 2012, BIOINFORMATICS, V28, P2537, DOI 10.1093/bioinformatics/bts460; Pereyra RT, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-70; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2015, CRYPTOGAMIE ALGOL, V36, P3, DOI 10.7872/crya.v36.iss1.2015.3; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Pillmann A, 1997, EUR J PHYCOL, V32, P379, DOI 10.1017/S0967026297001352; PIRY S, 1999, GENECLASS PROGRAM AS; Pritchard JK, 2000, GENETICS, V155, P945; Quillet MC, 1995, THEOR APPL GENET, V91, P1195, DOI 10.1007/BF00220929; Rannala B, 1997, P NATL ACAD SCI USA, V94, P9197, DOI 10.1073/pnas.94.17.9197; Rieseberg LH, 2001, TRENDS ECOL EVOL, V16, P351, DOI 10.1016/S0169-5347(01)02187-5; Rosenberg NA, 2004, MOL ECOL NOTES, V4, P137, DOI 10.1046/j.1471-8286.2003.00566.x; RUSSELL G, 1967, J MAR BIOL ASSOC UK, V47, P233, DOI 10.1017/S0025315400033695; RUSSELL G, 1983, MAR ECOL PROG SER, V11, P181, DOI 10.3354/meps011181; RUSSELL G, 1967, HELGOLAND WISS MEER, V15, P155, DOI 10.1007/BF01618619; Savoie AM, 2015, MOL ECOL, V24, P5927, DOI 10.1111/mec.13429; Schilthuizen M, 1999, P ROY SOC B-BIOL SCI, V266, P2181, DOI 10.1098/rspb.1999.0906; Serrao EA, 1999, J PHYCOL, V35, P382, DOI 10.1046/j.1529-8817.1999.3520382.x; SITES JW, 1987, SYST ZOOL, V36, P153, DOI 10.2307/2413266; Soltis PS, 2009, ANNU REV PLANT BIOL, V60, P561, DOI 10.1146/annurev.arplant.043008.092039; Spirito F, 1998, ENDLESS FORMS, P320; Stache B, 1990, EVOLUTIONARY BIOGEOG, P173; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Stebbins G. L., 1971, CHROMOSOMAL EVOLUTIO; STEBBINS GL, 1958, ADV GENET, V9, P147, DOI 10.1016/S0065-2660(08)60162-5; Turchetto C, 2015, AOB PLANTS, V7, DOI 10.1093/aobpla/plv084; Vaha JP, 2006, MOL ECOL, V15, P63, DOI 10.1111/j.1365-294X.2005.02773.x; VALERO M, 1992, TRENDS ECOL EVOL, V7, P25, DOI 10.1016/0169-5347(92)90195-H; WAGNER WH, 1987, BOT HELV, V97, P195; Wallace AL, 2004, J PHYCOL, V40, P1013, DOI 10.1111/j.1529-8817.2004.04085.x; WEIR BS, 1984, EVOLUTION, V38, P1358, DOI [10.2307/2408641, 10.1111/j.1558-5646.1984.tb05657.x]; Zardi GI, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0019402; Zuccarello GC, 2005, EUR J PHYCOL, V40, P337, DOI 10.1080/09670260500254743	86	12	12	0	13	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0962-1083	1365-294X		MOL ECOL	Mol. Ecol.	JUL	2017	26	13					3497	3512		10.1111/mec.14098			16	Biochemistry & Molecular Biology; Ecology; Evolutionary Biology	Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology	EY1AY	WOS:000403695500015	28295812				2021-04-07	
J	Lipinska, AP; Toda, NRT; Heesch, S; Peters, AF; Cock, JM; Coelho, SM				Lipinska, Agnieszka P.; Toda, Nicholas R. T.; Heesch, Svenja; Peters, Akira F.; Cock, J. Mark; Coelho, Susana M.			Multiple gene movements into and out of haploid sex chromosomes	GENOME BIOLOGY			English	Article						Sex chromosomes; Brown algae; Gene movement; Gene transposition	BROWN ALGA ECTOCARPUS; Y-CHROMOSOME; DROSOPHILA-MELANOGASTER; PHYLOGENETIC ANALYSIS; MOLECULAR PHYLOGENY; X-CHROMOSOMES; SEQUENCE DATA; EVOLUTION; GENOME; SELECTION	Background: Long-term evolution of sex chromosomes is a dynamic process shaped by gene gain and gene loss. Sex chromosome gene traffic has been studied in XY and ZW systems but no detailed analyses have been carried out for haploid phase UV sex chromosomes. Here, we explore sex-specific sequences of seven brown algal species to understand the dynamics of the sex-determining region (SDR) gene content across 100 million years of evolution. Results: A core set of sex-linked genes is conserved across all the species investigated, but we also identify modifications of both the U and the V SDRs that occurred in a lineage-specific fashion. These modifications involve gene loss, gene gain and relocation of genes from the SDR to autosomes. Evolutionary analyses suggest that the SDR genes are evolving rapidly and that this is due to relaxed purifying selection. Expression analysis indicates that genes that were acquired from the autosomes have been retained in the SDR because they confer a sex-specific role in reproduction. By examining retroposed genes in Saccharina japonica, we demonstrate that UV sex chromosomes have generated a disproportionate number of functional orphan retrogenes compared with autosomes. Movement of genes out of the UV sex chromosome could be a means to compensate for gene loss from the non-recombining region, as has been suggested for Y-derived retrogenes in XY sexual systems. Conclusion: This study provides the first analysis of gene traffic in a haploid UV system and identifies several features of general relevance to the evolution of sex chromosomes.	[Lipinska, Agnieszka P.; Toda, Nicholas R. T.; Heesch, Svenja; Cock, J. Mark; Coelho, Susana M.] UPMC Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, UMR 8227,Algal Genet Grp,Integrat Biol Marine Mod, CS 90074, F-29688 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France	Coelho, SM (corresponding author), UPMC Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, UMR 8227,Algal Genet Grp,Integrat Biol Marine Mod, CS 90074, F-29688 Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020	Cock, J. Mark/0000-0002-2650-0383; Heesch, Svenja/0000-0002-4531-0921	CNRSCentre National de la Recherche Scientifique (CNRS)European Commission; ANRFrench National Research Agency (ANR) [ANR-10-BTBR-04-01, ANR-12-JSV7-0008]; UPMC (Emergence Program); ERCEuropean Research Council (ERC)European Commission [638240]; GDRI 'Diversity, evolution and biotechnology of marine algae' [0803]	This work was supported by the CNRS, the ANR (ANR-10-BTBR-04-01; ANR-12-JSV7-0008), the UPMC (Emergence Program) and the ERC (grant agreement 638240). The GDRI 'Diversity, evolution and biotechnology of marine algae' (No 0803) supported the collaboration with Sylvain Faugeron. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Anders S, 2015, BIOINFORMATICS, V31, P166, DOI 10.1093/bioinformatics/btu638; Bachtrog D, 2004, NAT GENET, V36, P518, DOI 10.1038/ng1347; Bachtrog D, 2014, PLOS BIOL, V12, DOI 10.1371/journal.pbio.1001899; Bachtrog D, 2013, NAT REV GENET, V14, P113, DOI 10.1038/nrg3366; Bachtrog D, 2010, CURR BIOL, V20, P1476, DOI 10.1016/j.cub.2010.06.076; Bellott DW, 2010, NATURE, V466, P612, DOI 10.1038/nature09172; Bergero R, 2015, CURR BIOL, V25, P1234, DOI 10.1016/j.cub.2015.03.015; Betran E, 2002, GENOME RES, V12, P1854, DOI 10.1101/gr.6049; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; BULL JJ, 1978, AM NAT, V112, P245, DOI 10.1086/283267; Campos JL, 2012, GENOME BIOL EVOL, V4, P278, DOI 10.1093/gbe/evs010; Carelli FN, 2016, GENOME RES, V26, P301, DOI 10.1101/gr.198473.115; Carvalho AB, 2000, P NATL ACAD SCI USA, V97, P13239, DOI 10.1073/pnas.230438397; Carvalho AB, 2015, P NATL ACAD SCI USA, V112, P12450, DOI 10.1073/pnas.1516543112; Castresana J, 2000, MOL BIOL EVOL, V17, P540, DOI 10.1093/oxfordjournals.molbev.a026334; CHARLESWORTH B, 1987, AM NAT, V130, P113, DOI 10.1086/284701; Charlesworth B, 2000, PHILOS T R SOC B, V355, P1563, DOI 10.1098/rstb.2000.0717; Chikhi R, 2014, BIOINFORMATICS, V30, P31, DOI 10.1093/bioinformatics/btt310; Ciomborowska J, 2013, MOL BIOL EVOL, V30, P384, DOI 10.1093/molbev/mss235; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cortez D, 2014, NATURE, V508, P488, DOI 10.1038/nature13151; Dereeper A, 2008, NUCLEIC ACIDS RES, V36, pW465, DOI 10.1093/nar/gkn180; Emerson JJ, 2004, SCIENCE, V303, P537, DOI 10.1126/science.1090042; Eyre-Walker A, 1999, GENETICS, V152, P675; Galtier N, 2001, GENETICS, V159, P907; Gerrard DT, 2005, MOL BIOL EVOL, V22, P1423, DOI 10.1093/molbev/msi128; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Herpin A, 2015, EMBO REP, V16, P1260, DOI 10.15252/embr.201540667; Hughes JF, 2015, GENOME BIOL, V16, DOI 10.1186/s13059-015-0667-4; Immler S, 2015, EVOLUTION, V69, P694, DOI 10.1111/evo.12602; Ishizaki K, 2002, NUCLEIC ACIDS RES, V30, P4675, DOI 10.1093/nar/gkf604; Jakalski M, 2016, BIOL DIRECT, V11, DOI 10.1186/s13062-016-0138-1; Jordan CY, 2012, EVOLUTION, V66, P505, DOI 10.1111/j.1558-5646.2011.01448.x; Kawai H, 2016, J PHYCOL, V52, P682, DOI 10.1111/jpy.12419; Kawai H, 2015, J PHYCOL, V51, P918, DOI 10.1111/jpy.12332; Koerich LB, 2008, NATURE, V456, P949, DOI 10.1038/nature07463; Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]; Larracuente AM, 2013, GENETICS, V193, P201, DOI 10.1534/genetics.112.146167; Li WJ, 2013, EUKARYOT CELL, V12, P109, DOI 10.1128/EC.00249-12; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Lipinska AP, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140535; Luo RB, 2012, GIGASCIENCE, V1, DOI 10.1186/2047-217X-1-18; Luthringer R, 2015, PERSPECT PHYCOL, V1, P11; Luthringer R, 2015, MOL BIOL EVOL, V32, P2973, DOI 10.1093/molbev/msv173; Matsunaga S, 2003, MOL BIOL EVOL, V20, P1062, DOI 10.1093/molbev/msg114; Meisel RP, 2009, J MOL EVOL, V69, P81, DOI 10.1007/s00239-009-9254-1; Notredame C, 2000, J MOL BIOL, V302, P205, DOI 10.1006/jmbi.2000.4042; Pal C, 2001, GENETICS, V158, P927; Potrzebowski L, 2008, PLOS BIOL, V6, P709, DOI 10.1371/journal.pbio.0060080; Puigbo P, 2008, BIOL DIRECT, V3, DOI 10.1186/1745-6150-3-38; Salamov AA, 2000, GENOME RES, V10, P516, DOI 10.1101/gr.10.4.516; Shan TF, 2015, J APPL PHYCOL, V27, P1011, DOI 10.1007/s10811-014-0393-4; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Simpson JT, 2009, GENOME RES, V19, P1117, DOI 10.1101/gr.089532.108; Smeds L, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms8330; Sturgill D, 2007, NATURE, V450, P238, DOI 10.1038/nature06330; Subramanian S, 2004, GENETICS, V168, P373, DOI 10.1534/genetics.104.028944; Tao Y, 2007, PLOS BIOL, V5, P2576, DOI 10.1371/journal.pbio.0050293; Vibranovski MD, 2008, GENETICS, V179, P2325, DOI 10.1534/genetics.108.086819; Vicoso B, 2009, EVOLUTION, V63, P2413, DOI 10.1111/j.1558-5646.2009.00719.x; Wang J, 2012, J MOL EVOL, V74, P113, DOI 10.1007/s00239-012-9499-y; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986	64	21	21	0	13	BIOMED CENTRAL LTD	LONDON	236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND	1474-760X			GENOME BIOL	Genome Biol.	JUN 8	2017	18								104	10.1186/s13059-017-1201-7			14	Biotechnology & Applied Microbiology; Genetics & Heredity	Biotechnology & Applied Microbiology; Genetics & Heredity	EW9WI	WOS:000402869700001	28595587	DOAJ Gold, Green Published			2021-04-07	
J	Suutari, M; Leskinen, E; Spilling, K; Kostamo, K; Seppala, J				Suutari, Milla; Leskinen, Elina; Spilling, Kristian; Kostamo, Kirsi; Seppala, Jukka			Nutrient removal by biomass accumulation on artificial substrata in the northern Baltic Sea	JOURNAL OF APPLIED PHYCOLOGY			English	Article						Macroalgae; Invertebrates; Bioremediation; Eutrophication; Nutrient and heavy metal content; Baltic Sea	MULTI-TROPHIC AQUACULTURE; MUSSELS MYTILUS-EDULIS; CLADOPHORA-GLOMERATA; BIOGAS PRODUCTION; ARCHIPELAGO SEA; NEVA ESTUARY; MACROALGAE; SEAWEED; FINLAND; WATERS	We studied the potential of utilizing non-seeded artificial substrata in growing macrofauna and macroalgae to remove excess nutrients from seawater in the northern Baltic Sea. Succession, species composition, and biomass on two different substratum types were studied. Organic carbon (C), nitrogen (N), and phosphorus (P) as well as heavy metal (As, Cd, Cu, Pb, and Zn) contents were measured from the algal species and the invertebrate fraction. The invertebrate fraction formed more than 94% of the total biomass and consisted of blue mussels (Mytilus trossulus), bay barnacles (Amphibalanus improvisus), and hydroids (Cordylophora caspia and Gonothyraea loveni). Major algal species consisted of green algae Ulva spp. and Cladophora glomerata, red algae Ceramium tenuicorne and Polysiphonia fibrillosa, and filamentous brown algae Pylaiella littoralis and Ectocarpus siliculosus. The highest biomasses were measured at the end of the experiment, after 14.5 months of exposure in the sea, when the total biomass was on average 2.4 kg dry weight (DW) m(-2) substratum, containing approximately 50 g N and 5 g P. The heavy metal concentrations were site and species dependent and on average lower in the invertebrate fraction compared with the macroalgal fraction. In some cases especially, Cd may limit biomass use as fertilizers. According to these results, annual N and P emissions from small-sized point sources could be reduced by using artificial substrata to remove sessile organisms, but this would require extensive cultivations. Furthermore, the utilization of the marine macroalgal and invertebrate biomass in biogas production requires adding more carbon-rich feedstock to obtain optimal results.	[Suutari, Milla; Leskinen, Elina] Univ Helsinki, Dept Environm Sci, Helsinki, Finland; [Leskinen, Elina; Spilling, Kristian] Univ Helsinki, Tvarminne Zool Stn, Hango, Finland; [Spilling, Kristian; Kostamo, Kirsi; Seppala, Jukka] Finnish Environm Inst, Helsinki, Finland	Suutari, M (corresponding author), Univ Helsinki, Dept Environm Sci, Helsinki, Finland.	milla.suutari@helsinki.fi	Spilling, Kristian/L-7932-2014; Seppala, Jukka/L-5035-2014	Spilling, Kristian/0000-0002-8390-8270; Seppala, Jukka/0000-0002-1210-9893	European Regional Development Fund through the Baltic Sea Region Programme; Foundation for Research of Natural Resources in Finland; Maaja vesitekniikan tuki ry	This study was funded by European Regional Development Fund through the Baltic Sea Region Programme 2007-13 project "Sustainable Uses of Baltic Marine Resources, SUBMARINER," The Foundation for Research of Natural Resources in Finland, and Maaja vesitekniikan tuki ry. We thank T. Sara-Aho for the metal analysis; J. Saren, J. Koistinen, E. Nikkola, and M. Sjoblom for the nutrient analysis and other laboratory services; A. Ruuskanen, L. Jarvinen, V. Kinnunen, and G. Lundberg for the assistance with installations; and B. Regmi, B. Dulal, T. Saarinen, E. Salo, T. Hastings, H. Kauko, J. Enqvist, K. Yordanov, T. Hamalainen, and M. Issakainen for the assistance during field and laboratory work. Our sincere thanks for cooperation are due to S. Airaksinen and O. Norrdahl at the Finnish Game and Fisheries Institute. M. Riittonen kindly allowed us to use his boat and helped with the installation of the experiment.	Ahmed N, 2016, OCEAN COAST MANAGE, V132, P120, DOI 10.1016/j.ocecoaman.2016.08.017; [Anonymous], 2003, 172942 ISO; Berezina NA, 2008, J MARINE SYST, V74, pS80, DOI 10.1016/j.jmarsys.2008.03.027; Bergstrom L, 1999, NORD J BOT, V19, P375, DOI 10.1111/j.1756-1051.1999.tb01131.x; Brown M.T., 1998, METABOLISM TRACE MET, P185; Bruhn A, 2011, BIORESOURCE TECHNOL, V102, P2595, DOI 10.1016/j.biortech.2010.10.010; Bucholc K, 2014, SCI TOTAL ENVIRON, V473, P298, DOI 10.1016/j.scitotenv.2013.12.044; Carrilho ENVM, 2000, J ENVIRON MONITOR, V2, P410, DOI 10.1039/b004128i; CEDERWALL H, 1990, AMBIO, V19, P109; Chan SM, 2003, ARCH ENVIRON CON TOX, V44, P298, DOI 10.1007/s00244-002-2077-3; Chen Y, 2008, BIORESOURCE TECHNOL, V99, P4044, DOI 10.1016/j.biortech.2007.01.057; Chmielewska E, 2001, CROAT CHEM ACTA, V74, P135; Cranford PJ, 2013, AQUACULT ENV INTERAC, V4, P163, DOI 10.3354/aei00081; Davis TA, 2003, WATER RES, V37, P4311, DOI 10.1016/S0043-1354(03)00293-8; Dubrovskis V, 2012, ENG RUR DEVELOP, P566; Eklof JS, 2005, ESTUAR COAST SHELF S, V63, P385, DOI 10.1016/j.ecss.2004.11.014; Filipkowska A, 2008, OCEANOLOGIA, V50, P255; GAO K, 1994, J APPL PHYCOL, V6, P45, DOI 10.1007/BF02185904; Grote B, 2016, J APPL PHYCOL, V28, P3075, DOI 10.1007/s10811-016-0848-x; Grunewald N, 2009, BIOMACROMOLECULES, V10, P1155, DOI 10.1021/bm8014158; Gubelit YI, 2009, INLAND WATER BIOL, V2, P300, DOI 10.1134/S1995082909040026; Gubelit YI, 2010, MAR POLLUT BULL, V61, P183, DOI 10.1016/j.marpolbul.2010.02.013; Hadley S, 2015, J APPL PHYCOL, V27, P901, DOI 10.1007/s10811-014-0370-y; He PM, 2008, WATER RES, V42, P1281, DOI 10.1016/j.watres.2007.09.023; HELCOM, 2009, BALT SEA ENV P B, V115B; Hjerne O, 2002, LIMNOL OCEANOGR, V47, P1023, DOI 10.4319/lo.2002.47.4.1023; Huo YZ, 2011, J APPL PHYCOL, V23, P173, DOI 10.1007/s10811-010-9584-9; Jones AB, 2001, AQUACULTURE, V193, P155, DOI 10.1016/S0044-8486(00)00486-5; Jung KA, 2013, BIORESOURCE TECHNOL, V135, P182, DOI 10.1016/j.biortech.2012.10.025; Kangas P, 1986, MACROALGAE INDICATOR; Kersen P, 2013, THESIS; Kiirikki M, 1997, SARSIA, V82, P259, DOI 10.1080/00364827.1997.10413653; Koroleff F., 1983, METHODS SEAWATER ANA, P125; Korzen L, 2016, J APPL PHYCOL, V28, P1835, DOI 10.1007/s10811-015-0691-5; Kraufvelin P, 2007, ESTUAR COAST SHELF S, V72, P665, DOI 10.1016/j.ecss.2006.11.029; Kumari R, 2013, J APPL PHYCOL, V25, P1225, DOI 10.1007/s10811-012-9933-y; Lehvo A, 2001, AQUAT CONSERV, V11, P11, DOI 10.1002/aqc.428; LESKINEN E, 1984, OPHELIA, P137; Lindahl O, 2008, BIOSCIENCE EXPLAINED, V5, P1; Lundberg C, 2005, MAR POLLUT BULL, V50, P1185, DOI 10.1016/j.marpolbul.2005.04.029; Maar M, 2015, J MARINE SYST, V148, P48, DOI 10.1016/j.jmarsys.2015.02.003; Makinen T, 2008, FISHERY PREVENTIVE N; Martin G, 2006, J APPL PHYCOL, V18, P557, DOI 10.1007/s10811-006-9065-3; Mouritsen OG, 2013, J APPL PHYCOL, V25, P1777, DOI 10.1007/s10811-013-0014-7; Murphy AE, 2015, MAR ECOL PROG SER, V530, P135, DOI 10.3354/meps11301; NEUSHUL M, 1992, J APPL PHYCOL, V4, P255, DOI 10.1007/BF02161211; Nielsen MM, 2012, J APPL PHYCOL, V24, P449, DOI 10.1007/s10811-011-9767-z; Nkemka VN, 2012, PROCESS BIOCHEM, V47, P2523, DOI 10.1016/j.procbio.2012.06.022; Nobrega JA, 2012, TALANTA, V98, P272, DOI 10.1016/j.talanta.2012.06.079; Patel S, 2012, BIO-TECHNOL, V2, P171, DOI DOI 10.1007/S13205-012-0061-9; Pedersen MF, 1997, MAR ECOL PROG SER, V161, P155, DOI 10.3354/meps161155; Perez-Camacho A, 2013, AQUACULTURE, V406, P172, DOI 10.1016/j.aquaculture.2013.05.019; Peu P, 2011, BIORESOURCE TECHNOL, V102, P10794, DOI 10.1016/j.biortech.2011.08.096; Protasowicki M, 2008, ENVIRON MONIT ASSESS, V141, P329, DOI 10.1007/s10661-007-9899-4; Rebours C, 2014, J APPL PHYCOL, V26, P1939, DOI 10.1007/s10811-014-0304-8; Reddy C, 2008, J APPL PHYCOL, V20, P609, DOI 10.1007/s10811-007-9205-4; Reid GK, 2010, AQUACULTURE, V299, P165, DOI 10.1016/j.aquaculture.2009.12.002; Richardson CA, 2001, MAR ECOL PROG SER, V211, P157, DOI 10.3354/meps211157; Riekie GJ, 2006, CHEMOSPHERE, V65, P332, DOI 10.1016/j.chemosphere.2006.02.025; Risen E, 2014, J RENEW SUSTAIN ENER, V6, DOI 10.1063/1.4862783; Roesijadi G., 2010, MACROALGAE BIOMASS F; Rossner Y, 2014, J APPL PHYCOL, V26, P753, DOI 10.1007/s10811-014-0269-7; Roos C, 2003, NORD J BOT, V23, P247, DOI 10.1111/j.1756-1051.2003.tb00387.x; Salovius S, 2004, OPHELIA, V58, P65, DOI 10.1080/00785326.2004.10410214; Seghetta M, 2014, ECOL MODEL, V288, P25, DOI 10.1016/j.ecolmodel.2014.05.006; Shahbazi F., 2015, International Journal of Agriculture and Crop Sciences (IJACS), V8, P283; Sheng PX, 2004, J COLLOID INTERF SCI, V275, P131, DOI 10.1016/j.jcis.2004.01.036; SOLORZANO L, 1980, LIMNOL OCEANOGR, V25, P754, DOI 10.4319/lo.1980.25.4.0754; Suutari M, 2015, PHYCOL RES, V63, P1, DOI 10.1111/pre.12078; USEPA, 2007, 3051A USEPA; Vahteri P, 2000, AMBIO, V29, P338, DOI 10.1639/0044-7447(2000)029[0338:ADAMCT]2.0.CO;2; Vallius Henry, 1999, Boreal Environment Research, V4, P19; Voigt Heinz-Rudolf, 2007, Proceedings of the Estonian Academy of Sciences Biology Ecology, V56, P224; Wallin M, 1992, LOAD MODELS NUTR COA; Weldrick CK, 2016, J MARINE SYST, V157, P118, DOI 10.1016/j.jmarsys.2016.01.001; Wu CY, 2004, HYDROBIOLOGIA, V512, P153, DOI 10.1023/B:HYDR.0000020321.73728.21; Wulff F, 2014, AMBIO, V43, P11, DOI 10.1007/s13280-013-0484-5; Xu B, 2009, J APPL PHYCOL, V21, P171, DOI 10.1007/s10811-008-9347-z; Zhou Y, 2006, AQUACULTURE, V252, P264, DOI 10.1016/j.aquaculture.2005.06.046	79	6	6	2	66	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0921-8971	1573-5176		J APPL PHYCOL	J. Appl. Phycol.	JUN	2017	29	3					1707	1720		10.1007/s10811-016-1023-0			14	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	EV0KU	WOS:000401429300058					2021-04-07	
J	Hirth, M; Liverani, S; Mahlow, S; Bouget, FY; Pohnert, G; Sasso, S				Hirth, Matthias; Liverani, Silvia; Mahlow, Sebastian; Bouget, Francois-Yves; Pohnert, Georg; Sasso, Severin			Metabolic profiling identifies trehalose as an abundant and diurnally fluctuating metabolite in the microalga Ostreococcus tauri	METABOLOMICS			English	Article						Microalgae; Picoeukaryotes; Carbon metabolism; Untargeted metabolomics; pantothenate	ALGA ECTOCARPUS-SILICULOSUS; PICOEUKARYOTE OSTREOCOCCUS; GREEN-ALGA; EUKARYOTIC PHYTOPLANKTON; PICOALGA OSTREOCOCCUS; INSIGHTS; PLANTS; PRASINOPHYCEAE; TRANSCRIPTION; CHLOROPHYTA	Introduction The picoeukaryotic alga Ostreococcus tauri (Chlorophyta) belongs to the widespread group of marine prasinophytes. Despite its ecological importance, little is known about the metabolism of this alga. Objectives In this work, changes in the metabolome were quantified when O. tauri was grown under alternating cycles of 12 h light and 12 h darkness. Methods Algal metabolism was analyzed by gas chromatography-mass spectrometry. Using fluorescence-activated cell sorting, the bacteria associated with O. tauri were depleted to below 0.1% of total cells at the time of metabolic profiling. Results Of 111 metabolites quantified over light-dark cycles, 20 (18%) showed clear diurnal variations. The strongest fluctuations were found for trehalose. With an intracellular concentration of 1.6 mM in the dark, this disaccharide was six times more abundant at night than during the day. This fluctuation pattern of trehalose may be a consequence of starch degradation or of the synchronized cell cycle. On the other hand, maltose (and also sucrose) was below the detection limit (similar to 10 mu M). Accumulation of glycine in the light is in agreement with the presence of a classical glycolate pathway of photorespiration. We also provide evidence for the presence of fatty acid methyl and ethyl esters in O. tauri. Conclusions This study shows how the metabolism of O. tauri adapts to day and night and gives new insights into the configuration of the carbon metabolism. In addition, several less common metabolites were identified.	[Hirth, Matthias; Mahlow, Sebastian; Sasso, Severin] Friedrich Schiller Univ, Inst Gen Bot & Plant Physiol, Jena, Germany; [Liverani, Silvia] Brunel Univ London, Dept Math, Uxbridge, Middx, England; [Bouget, Francois-Yves] UPMC Univ Paris 06, Sorbonne Univ, Banyuls Sur Mer, France; [Bouget, Francois-Yves] CNRS, UMR 7621, Lab Oceanog Microbienne, Observ Oceanol, Banyuls Sur Mer, France; [Pohnert, Georg] Friedrich Schiller Univ, Inst Inorgan & Analyt Chem, Jena, Germany; [Pohnert, Georg] Max Planck Inst Chem Ecol, Jena, Germany	Sasso, S (corresponding author), Friedrich Schiller Univ, Inst Gen Bot & Plant Physiol, Jena, Germany.	severin.sasso@uni-jena.de	Pohnert, Georg/D-3721-2013	Pohnert, Georg/0000-0003-2351-6336; Sasso, Severin/0000-0002-2226-845X	Jena School for Microbial Communication [GSC 214/2]; German Research Foundation (DFG)German Research Foundation (DFG); DFGGerman Research Foundation (DFG)European Commission [SFB 1127]	We are grateful to Katharina Eick, Constanze Kuhlisch and Christine Lembke (Friedrich Schiller University, Jena) for help with GC-MS and subsequent data analysis, and to Andrea Volpel, Yvonne Schlenker, Prof. Bernd Sigusch, and Dr. Martin Forster (University Hospital, Jena) for help with flow cytometry and access to their instruments. M.H. and S.S. acknowledge financial support by the Jena School for Microbial Communication (GSC 214/2), which is funded by the German Research Foundation (DFG). G.P. and S.S. are supported by the DFG-funded Collaborative Research Centre ChemBioSys (SFB 1127).	Abby SS, 2014, FRONT MICROBIOL, V5, DOI 10.3389/fmicb.2014.00505; Amin SA, 2012, MICROBIOL MOL BIOL R, V76, P667, DOI 10.1128/MMBR.00007-12; Herrera-Valencia VA, 2012, ANN MICROBIOL, V62, P865, DOI 10.1007/s13213-011-0361-z; Blanc-Mathieu R, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-1103; Blanc-Mathieu R, 2013, GENOME BIOL EVOL, V5, P1503, DOI 10.1093/gbe/evt106; Botebol H, 2015, P NATL ACAD SCI USA, V112, P14652, DOI 10.1073/pnas.1506074112; Broeckling CD, 2006, ANAL CHEM, V78, P4334, DOI 10.1021/ac0521596; Cardol P, 2008, P NATL ACAD SCI USA, V105, P7881, DOI 10.1073/pnas.0802762105; CHRETIENNOTDINET MJ, 1995, PHYCOLOGIA, V34, P285, DOI 10.2216/i0031-8884-34-4-285.1; Collado-Fabbri S, 2011, LIMNOL OCEANOGR, V56, P2334, DOI 10.4319/lo.2011.56.6.2334; Corellou F, 2009, PLANT CELL, V21, P3436, DOI 10.1105/tpc.109.068825; COURTIES C, 1994, NATURE, V370, P255, DOI 10.1038/370255a0; Debolt S, 2007, ANN BOT-LONDON, V99, P3, DOI 10.1093/aob/mcl236; Derelle E, 2006, P NATL ACAD SCI USA, V103, P11647, DOI 10.1073/pnas.0604795103; Derelle E, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002250; Djouani-Tahri EB, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0028471; Djouani-Tahri EB, 2011, PLANT J, V65, P578, DOI 10.1111/j.1365-313X.2010.04444.x; Ewald JC, 2016, MOL CELL, V62, P532, DOI 10.1016/j.molcel.2016.02.017; Falkowski PG, 1998, SCIENCE, V281, P200, DOI 10.1126/science.281.5374.200; Farinas B, 2006, PLANT MOL BIOL, V60, P277, DOI 10.1007/s11103-005-4066-1; Gibon Y, 2006, GENOME BIOL, V7, DOI 10.1186/gb-2006-7-8-r76; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Grimsley N, 2010, MOL BIOL EVOL, V27, P47, DOI 10.1093/molbev/msp203; Hansen H. P., 1999, METHODS SEAWATER ANA; Heijde M, 2010, PLANT CELL ENVIRON, V33, P1614, DOI 10.1111/j.1365-3040.2010.02168.x; Henderson GP, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000749; Hortensteiner S, 2011, BBA-BIOENERGETICS, V1807, P977, DOI 10.1016/j.bbabio.2010.12.007; Hom EFY, 2015, TRENDS PLANT SCI, V20, P689, DOI 10.1016/j.tplants.2015.09.004; KELLER MD, 1987, J PHYCOL, V23, P633; Kim SG, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0026214; Kolbe A, 2005, P NATL ACAD SCI USA, V102, P11118, DOI 10.1073/pnas.0503410102; Kuhn ML, 2009, J BIOL CHEM, V284, P34092, DOI 10.1074/jbc.M109.037614; Kusari P, 2016, RSC ADV, V6, P10011, DOI 10.1039/c5ra25042k; LEE ML, 1979, ANAL CHEM, V51, P768, DOI 10.1021/ac50042a043; Lelandais G, 2016, BMC GENOMICS, V17, DOI 10.1186/s12864-016-2666-6; Leliaert F, 2012, CRIT REV PLANT SCI, V31, P1, DOI 10.1080/07352689.2011.615705; LI WKW, 1994, LIMNOL OCEANOGR, V39, P169, DOI 10.4319/lo.1994.39.1.0169; Lozano JC, 2014, PLANT J, V78, P1073, DOI 10.1111/tpj.12530; Lunn JE, 2014, PLANT J, V79, P544, DOI 10.1111/tpj.12509; Lupette J, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.01414; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Monnier A, 2010, BMC GENOMICS, V11, DOI 10.1186/1471-2164-11-192; Moulager M, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1000957; O'Kelly CJ, 2003, J PHYCOL, V39, P850, DOI 10.1046/j.1529-8817.2003.02201.x; O'Neill JS, 2011, NATURE, V469, P554, DOI 10.1038/nature09654; Paerl RW, 2017, ISME J, V11, P753, DOI 10.1038/ismej.2016.145; Paul MJ, 2008, ANNU REV PLANT BIOL, V59, P417, DOI 10.1146/annurev.arplant.59.032607.092945; Ramanan R, 2016, BIOTECHNOL ADV, V34, P14, DOI 10.1016/j.biotechadv.2015.12.003; Rodriguez F, 2005, ENVIRON MICROBIOL, V7, P853, DOI 10.1111/j.1462-2920.2005.00758.x; Sato S, 2008, BMC SYST BIOL, V2, DOI 10.1186/1752-0509-2-51; Smith AG, 2007, CURR OPIN PLANT BIOL, V10, P266, DOI 10.1016/j.pbi.2007.04.009; Sorokina O, 2011, BMC SYST BIOL, V5, DOI 10.1186/1752-0509-5-36; Streb Sebastian, 2012, Arabidopsis Book, V10, pe0160, DOI 10.1199/tab.0160; Tapia H, 2014, CURR BIOL, V24, P2758, DOI 10.1016/j.cub.2014.10.005; Tardif M, 2012, MOL BIOL EVOL, V29, P3625, DOI 10.1093/molbev/mss178; Vaezi R, 2013, MAR DRUGS, V11, P5116, DOI 10.3390/md11125116; Vaulot D, 2008, FEMS MICROBIOL REV, V32, P795, DOI 10.1111/j.1574-6976.2008.00121.x; Vidoudez C, 2012, METABOLOMICS, V8, P654, DOI 10.1007/s11306-011-0356-6; Weirauch MT, 2014, CELL, V158, P1431, DOI 10.1016/j.cell.2014.08.009; Worden AZ, 2015, SCIENCE, V347, DOI 10.1126/science.1257594; Worden AZ, 2004, LIMNOL OCEANOGR, V49, P168, DOI 10.4319/lo.2004.49.1.0168	61	16	16	1	31	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	1573-3882	1573-3890		METABOLOMICS	Metabolomics	JUN	2017	13	6							68	10.1007/s11306-017-1203-1			14	Endocrinology & Metabolism	Endocrinology & Metabolism	EV4EN	WOS:000401711400003	28473745	Green Published, Other Gold			2021-04-07	
J	Gachon, CMM; Strittmatter, M; Badis, Y; Fletcher, KI; Van West, P; Muller, DG				Gachon, Claire M. M.; Strittmatter, Martina; Badis, Yacine; Fletcher, Kyle I.; Van West, Pieter; Mueller, Dieter G.			Pathogens of brown algae: culture studies of Anisolpidium ectocarpii and A-rosenvingei reveal that the Anisolpidiales are uniflagellated oomycetes	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						18S rRNA; Anisolpidiales; cox1; cox2; Ectocarpus; host range; Hyphochytriales; Pylaiella	SP-NOV; LITTORALIS ECTOCARPALES; PYLAIELLA-LITTORALIS; MOLECULAR PHYLOGENY; INFECTS BOSTRYCHIA; OLPIDIOPSIS SP.; RED ALGAE; EURYCHASMA; ORGANIZATION; PARASITE	Using laboratory cultures, we have documented the life cycle of Anisolpidium ectocarpii, a pathogen of Ectocarpus and other filamentous brown algae, and presented preliminary observations on Anisolpidium rosenvingei, a pathogen of Pylaiella littoralis. Consistent with earlier reports, the zoospores of both species have a single anterior flagellum, which justified the placement of Anisolpidium amongst the Hyphochytriales (Hyphochytridiomycota). We have also shown that A. ectocarpii can complete its infection cycle in a broad selection of species from various brown algal orders, whereas A. rosenvingei seemingly exhibits a strict specificity for unilocular sporangia of P. littoralis. Unexpectedly, nuclear (18S rRNA) and mitochondrial (cox1, cox2) markers regroup A. ectocarpii and A. rosenvingei, into a hitherto unrecognized monophyletic clade within the oomycetes (Oomycota), most closely related to the Olpidiopsidales. The Anisolpidium genus is therefore entirely distinct from the Hyphochytridiomycota and represents the first confirmed instance of an anteriorly uniciliate oomycete. Finally, we suggest that a valid morphological criterion to separate true hyphochytrids from oomycetes is the timing of zoospore cleavage. Given the evidence, we propose to transfer the Anisolpidiales from the Hyphochytriales to the Oomycetes.	[Gachon, Claire M. M.; Strittmatter, Martina; Badis, Yacine] Scottish Assoc Marine Sci, Culture Collect Algae & Protozoa, Scottish Marine Inst, Oban PA37 1QA, Argyll, Scotland; [Fletcher, Kyle I.; Van West, Pieter] Univ Aberdeen, Inst Med Sci, Aberdeen Oomycete Lab, Foresterhill AB25 2ZD, Scotland; [Mueller, Dieter G.] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany	Gachon, CMM (corresponding author), Scottish Assoc Marine Sci, Culture Collect Algae & Protozoa, Scottish Marine Inst, Oban PA37 1QA, Argyll, Scotland.	claire.gachon@sams.ac.uk		Strittmatter, Martina/0000-0002-1258-9751; Badis, Yacine/0000-0003-1606-3906; Gachon, Claire/0000-0002-3702-7472	Natural Environment Research Council: Genomia Ltd: Seventh Framework Programme [NE/J00460X/1, NE/L013223/1, HERDIR, PERG03-GA-2008-230865]; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/M026566/1] Funding Source: researchfish; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/J00460X/1, NE/L013223/1, NE/F012578/1, NE/L013029/1] Funding Source: researchfish	This work was supported by the Natural Environment Research Council: Genomia Ltd: Seventh Framework Programme [Grant Number NE/J00460X/1, NE/L013223/1, HERDIR, PERG03-GA-2008-230865].	Adachi J, 1996, J MOL EVOL, V42, P459; Adl SM, 2012, J EUKARYOT MICROBIOL, V59, P429, DOI 10.1111/j.1550-7408.2012.00644.x; Beakes GW, 2012, PROTOPLASMA, V249, P3, DOI 10.1007/s00709-011-0269-2; Beakes GW, 1998, FUNGAL GENET BIOL, V24, P45, DOI 10.1006/fgbi.1998.1072; BEAKES GW, 2014, SYSTEMATICS EVOLUTIO, P39, DOI DOI 10.1007/978-3-642-55318-9_3; Dick MW., 2001, STRAMINIPILOUS FUNGI; Fletcher K, 2015, DIS AQUAT ORGAN, V117, P45, DOI 10.3354/dao02930; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Hall TA.., 1999, NUCL ACIDS S SERIES, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Hausner G, 2000, CAN J BOT, V78, P124, DOI 10.1139/b99-177; Hudspeth DSS, 2000, MYCOLOGIA, V92, P674, DOI 10.2307/3761425; JOHNSON TW, 1957, AM J BOT, V44, P875, DOI 10.2307/2438908; Karling JS, 1939, AM J BOT, V26, P512, DOI 10.2307/2436575; Karling JS, 1943, AM J BOT, V30, P637, DOI 10.2307/2437479; Karling JS., 1977, CHYTRIDIOMYCETARUM I; KATSAROS C, 1992, PROTOPLASMA, V169, P75, DOI 10.1007/BF01343372; Kawai H., 1992, KOREAN J PHYCOLOGY, V7, P33; Kearse M, 2012, BIOINFORMATICS, V28, P1647, DOI 10.1093/bioinformatics/bts199; Kim GH, 2014, ALGAE-SEOUL, V29, P249, DOI 10.4490/algae.2014.29.4.249; Klochkova TA, 2016, J APPL PHYCOL, V28, P73, DOI 10.1007/s10811-015-0595-4; Klochkova TA, 2012, J APPL PHYCOL, V24, P135, DOI 10.1007/s10811-011-9661-8; Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msv279, 10.1093/molbev/msw054]; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Le SQ, 2008, MOL BIOL EVOL, V25, P1307, DOI 10.1093/molbev/msn067; Lin YC, 2012, APPL ENVIRON MICROB, V78, P3387, DOI 10.1128/AEM.06952-11; Loureiro R, 2015, NEW PHYTOL, V206, P489, DOI 10.1111/nph.13278; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; Maier I, 1998, EUR J PHYCOL, V33, P213, DOI 10.1017/S0967026298001747; Marano AV, 2012, MAR FRESHW BOTANY, P167; Massana R, 2004, APPL ENVIRON MICROB, V70, P3528, DOI 10.1128/AEM.70.6.3528-3534.2004; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; Neuhauser S., 2014, BMC EVOLUTIONARY BIO, V14, P1; Neuhauser S, 2011, MAR FRESHWATER RES, V62, P365, DOI 10.1071/MF10282; Robideau GP, 2011, MOL ECOL RESOUR, V11, P1002, DOI 10.1111/j.1755-0998.2011.03041.x; Sekimoto S, 2008, MYCOL RES, V112, P361, DOI 10.1016/j.mycres.2007.11.002; Sekimoto S, 2009, PHYCOLOGIA, V48, P460, DOI 10.2216/08-11.1; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES; Strittmatter M, 2013, DIS AQUAT ORGAN, V104, P1, DOI 10.3354/dao02583; TAMURA K, 1992, MOL BIOL EVOL, V9, P678; Tsirigoti A, 2014, PLANT BIOLOGY, V16, P272, DOI 10.1111/plb.12041; Tsirigoti A, 2015, PROTOPLASMA, V252, P845, DOI 10.1007/s00709-014-0721-1; West JA, 2006, PHYCOL RES, V54, P72, DOI 10.1111/j.1440-1835.2006.00410.x; WHITTICK A, 1972, ARCH MIKROBIOL, V82, P353, DOI 10.1007/BF00424938	44	12	15	1	12	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0967-0262	1469-4433		EUR J PHYCOL	Eur. J. Phycol.	MAY	2017	52	2					133	148		10.1080/09670262.2016.1252857			16	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	ES6HN	WOS:000399647500001					2021-04-07	
J	Terauchi, M; Yamagishi, T; Hanyuda, T; Kawai, H				Terauchi, Makoto; Yamagishi, Takahiro; Hanyuda, Takeaki; Kawai, Hiroshi			Genome-wide computational analysis of the secretome of brown algae (Phaeophyceae)	MARINE GENOMICS			English	Article						Alginate; Carbohydrate binding module (CBM); Extracellular matrix (ECM); Laminariales; Metalloproteinase (Metzincin); RNA-seq	EXTRACELLULAR-MATRIX; LIFE-HISTORY; CELL-WALLS; MANNURONAN C5-EPIMERASE; MOLECULAR PHYLOGENY; SECONDARY STRUCTURE; EVOLUTION; PROTEIN; EXPRESSION; ALGINATE	Brown algae have evolved complex multicellularity in the heterokont lineage. They are phylogenetically distant to land plants, fungi and animals. Especially, the members of Laminariales (so-called kelps) have developed highly differentiated tissues. Extracellular matrix (ECM) plays pivotal roles in a number of essential processes in multicellular organisms, such as cell adhesion, cell and tissue differentiations, cell-to-cell communication, and responses to environmental stimuli. In these processes, a set of extracellular secreted proteins called the secretome operates remodeling of the physicochemical nature of ECM and signal transduction by interacting with cell surface proteins and signaling molecules. Characterization of the secretome is a critical step to clarify the contributions of ECM to the multicellularity of brown algae. However, the identity of the brown algal secretome has been poorly understood. In order to reveal the repertory of the brown algal secretome and its involvement in the evolution of Laminariales, we conducted a genome-wide analysis of the brown algal secretome utilizing the published complete genome data of Ectocarpus siliculosus and Saccharina japonica as well as newly obtained RNA-seq data of seven laminarialean species (Agarum clathratum, Alaria crassifolia, Aureophycus aleuticus, Costaria costata, Pseudochorda nagaii, Saccharina angustata and Undaria pinnatlfida) largely covering the laminarialean families. We established the in silico pipeline to systematically and accurately detect the secretome by combining multiple prediction algorithms for the N-terminal signal peptide and transmembrane domain within the protein sequence. From 16,189 proteins of E. siliculosus and 18,733 proteins of S. japonica, 552 and 964 proteins respectively were predicted to be classified as the secretome. Conserved domain analysis showed that the domain repertory were very similar to each other, and that of the brown algal secretome was partially common with that of the secretome of other multicellular organisms (land plants, fungi and animals). In the laminarialean species, it was estimated that the gene abundance and the domain architecture of putative ECM remodeling-related proteins were altered compared with those of E. siliculosus, and that the alteration started from the basal group of Laminariales. These results suggested that brown algae have developed their own secretome, and its functions became more elaborated in the more derived members in Laminariales. (C) 2016 Elsevier B.V. All rights reserved.	[Terauchi, Makoto] Kobe Univ, Org Adv & Integrated Res, Kobe, Hyogo 6578501, Japan; [Yamagishi, Takahiro; Hanyuda, Takeaki; Kawai, Hiroshi] Kobe Univ, Res Ctr Inland Seas, Kobe, Hyogo 6578501, Japan; [Yamagishi, Takahiro] Natl Inst Environm Studies, Ctr Hlth & Environm Risk Res, Tsukuba, Ibaraki 3058506, Japan	Terauchi, M (corresponding author), Kobe Univ, Org Adv & Integrated Res, Kobe, Hyogo 6578501, Japan.	terauchil117@gmail.com			Japan Society for Promotion of SciencesMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [25291087]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [17K07533, 16H04832] Funding Source: KAKEN	We are grateful to Dr. Eric C. Henry for valuable comments on the manuscript, and Drs. Taizo Motomura, Chikako Nagasato, Atsuko Tanaka and Hiroki Kawamoto (Muroran Marine Station, Hokkaido University) for their help in collecting the field samples. A part of this study was supported by a scientific research grant from the Japan Society for Promotion of Sciences (Project Number: 25291087) to H.K.	Abbott DW, 2007, J MOL BIOL, V367, P1023, DOI 10.1016/j.jmb.2007.01.030; Agrawal GK, 2010, PROTEOMICS, V10, P799, DOI 10.1002/pmic.200900514; Apte SS, 2009, J BIOL CHEM, V284, P31493, DOI 10.1074/jbc.R109.052340; Badur AH, 2015, APPL ENVIRON MICROB, V81, P1856, DOI 10.1128/AEM.03460-14; Bold H.C., 1985, STRUCTURE REPROD SEC, P720; Brew K, 2010, BBA-MOL CELL RES, V1803, P55, DOI 10.1016/j.bbamcr.2010.01.003; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dunwell JM, 2004, PHYTOCHEMISTRY, V65, P7, DOI 10.1016/j.phytochem.2003.08.016; Fischl R., 2016, GLYCOBIOLOGY; Frantz C, 2010, J CELL SCI, V123, P4195, DOI 10.1242/jcs.023820; Georgelis N, 2011, J BIOL CHEM, V286, P16814, DOI 10.1074/jbc.M111.225037; Gomis-Ruth FX, 2003, MOL BIOTECHNOL, V24, P157, DOI 10.1385/MB:24:2:157; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Graham L.E., 2000, ALGAE-SEOUL, P640; Gruber A, 2015, PLANT J, V81, P519, DOI 10.1111/tpj.12734; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Guillen D, 2010, APPL MICROBIOL BIOT, V85, P1241, DOI 10.1007/s00253-009-2331-y; Han WJ, 2016, APPL ENVIRON MICROB, V82, P364, DOI 10.1128/AEM.03022-15; HAUG A, 1974, CARBOHYD RES, V32, P217, DOI 10.1016/S0008-6215(00)82100-X; Hirokawa T, 1998, BIOINFORMATICS, V14, P378, DOI 10.1093/bioinformatics/14.4.378; Hynes RO, 2009, SCIENCE, V326, P1216, DOI 10.1126/science.1176009; Inoue A, 2016, ALGAL RES, V16, P282, DOI 10.1016/j.algal.2016.03.030; Jenkins J, 1998, J STRUCT BIOL, V122, P236, DOI 10.1006/jsbi.1998.3985; Jones P, 2014, BIOINFORMATICS, V30, P1236, DOI 10.1093/bioinformatics/btu031; Kall L, 2004, J MOL BIOL, V338, P1027, DOI 10.1016/j.jmb.2004.03.016; Kawai H, 2001, J PHYCOL, V37, P130, DOI 10.1046/j.1529-8817.1999.014012130.x; KAWAI H, 1985, PHYCOLOGIA, V24, P289, DOI 10.2216/i0031-8884-24-3-289.1; KAWAI H, 1986, J PHYCOL, V22, P286, DOI 10.1111/j.1529-8817.1986.tb00025.x; Kawai H., 2014, PERSPECT PHYCOL, V1, P27; Kawai H, 2015, J PHYCOL, V51, P918, DOI 10.1111/jpy.12332; Kawai H, 2013, SCI REP-UK, V3, DOI 10.1038/srep02491; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Krogh A, 2001, J MOL BIOL, V305, P567, DOI 10.1006/jmbi.2000.4315; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lespinet O, 2002, GENOME RES, V12, P1048, DOI 10.1101/gr.174302; Lu PF, 2011, CSH PERSPECT BIOL, V3, DOI 10.1101/cshperspect.a005058; Lum G., 2014, COMPUT MOL BIOL, V4, P1, DOI [10.5376/cmb.2014.04.0001, DOI 10.5376/CMB.2014.04.0001]; Lum G, 2011, PLANT OMICS, V4, P114; Lum G, 2011, DATABASE-OXFORD, DOI 10.1093/database/bar001; MABEAU S, 1987, J EXP BOT, V38, P1573, DOI 10.1093/jxb/38.9.1573; Marchler-Bauer A, 2015, NUCLEIC ACIDS RES, V43, pD222, DOI 10.1093/nar/gku1221; MATTHEWS BW, 1975, BIOCHIM BIOPHYS ACTA, V405, P442, DOI 10.1016/0005-2795(75)90109-9; Meinken J, 2014, COMPUT MOL BIOL, P4, DOI [10.5376/cmb.2014.04.0007, DOI 10.5376/CMB.2014.04.0007]; Meinken J., 2015, DATABASE; Meng Q, 1999, J BIOL CHEM, V274, P10184, DOI 10.1074/jbc.274.15.10184; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Miller IJ, 1996, PHYTOCHEMISTRY, V41, P1315, DOI 10.1016/0031-9422(95)00741-5; Min XJ, 2010, J PROTEOM BIOINFORM, V3, P143, DOI DOI 10.4172/JPB.1000133; Misner I, 2015, GENOME BIOL EVOL, V7, P120, DOI 10.1093/gbe/evu276; MUNRO S, 1986, CELL, V46, P291, DOI 10.1016/0092-8674(86)90746-4; Nardi CF, 2015, PLANT MOL BIOL, V88, P101, DOI 10.1007/s11103-015-0311-4; Nikolaidis N, 2014, MOL BIOL EVOL, V31, P376, DOI 10.1093/molbev/mst206; Nishitsuji K., 2016, DNA RES; Obembe OO, 2007, J PLANT RES, V120, P605, DOI 10.1007/s10265-007-0099-7; Ozbek S, 2010, MOL BIOL CELL, V21, P4300, DOI 10.1091/mbc.E10-03-0251; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Prochnik SE, 2010, SCIENCE, V329, P223, DOI 10.1126/science.1188800; Reynolds SM, 2008, PLOS COMPUT BIOL, V4, DOI 10.1371/journal.pcbi.1000213; Salgado LT, 2009, J PHYCOL, V45, P193, DOI 10.1111/j.1529-8817.2008.00642.x; Sampedro J, 2005, GENOME BIOL, V6, DOI 10.1186/gb-2005-6-12-242; SCHMITZ K, 1990, SIEVE ELEMENTS, P1; SHIRAIWA Y, 1975, BOT MAR, V18, P97, DOI 10.1515/botm.1975.18.2.97; Sigrist Christian J A, 2002, Brief Bioinform, V3, P265, DOI 10.1093/bib/3.3.265; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Srivastava R, 2008, PLANT J, V56, P219, DOI 10.1111/j.1365-313X.2008.03598.x; Strittmatter M, 2016, PLANT CELL ENVIRON, V39, P259, DOI 10.1111/pce.12533; Tatewaki M, 1966, PHYCOLOGIA, V6, P62, DOI DOI 10.2216/I0031-8884-6-1-62.1; Terauchi M., 2016, ECTOCARPALES PHAEOPH; Tonon T, 2008, J PHYCOL, V44, P1250, DOI 10.1111/j.1529-8817.2008.00580.x; VONHEIJNE G, 1990, J MEMBRANE BIOL, V115, P195, DOI 10.1007/BF01868635; Wallin E, 1998, PROTEIN SCI, V7, P1029, DOI 10.1002/pro.5560070420; Weiner RM, 2008, PLOS GENET, V4, DOI 10.1371/journal.pgen.1000087; Williams F, 2014, MATRIX BIOL, V37, P60, DOI 10.1016/j.matbio.2014.02.002; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Yin YB, 2012, NUCLEIC ACIDS RES, V40, pW445, DOI 10.1093/nar/gks479	79	11	11	0	25	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1874-7787	1876-7478		MAR GENOM	Mar. Genom.	APR	2017	32						49	59		10.1016/j.margen.2016.12.002			11	Genetics & Heredity; Marine & Freshwater Biology	Genetics & Heredity; Marine & Freshwater Biology	ES4QL	WOS:000399519700007	28063828				2021-04-07	
J	Cormier, A; Avia, K; Sterck, L; Derrien, T; Wucher, V; Andres, G; Monsoor, M; Godfroy, O; Lipinska, A; Perrineau, MM; Van De Peer, YV; Hitte, C; Corre, E; Coelho, SM; Cock, JM				Cormier, Alexandre; Avia, Komlan; Sterck, Lieven; Derrien, Thomas; Wucher, Valentin; Andres, Gwendoline; Monsoor, Misharl; Godfroy, Olivier; Lipinska, Agnieszka; Perrineau, Marie-Mathilde; Van De Peer, Yves; Hitte, Christophe; Corre, Erwan; Coelho, Susana M.; Cock, J. Mark			Re-annotation, improved large-scale assembly and establishment of a catalogue of noncoding loci for the genome of the model brown alga Ectocarpus	NEW PHYTOLOGIST			English	Article						alternative splicing; brown algae; Ectocarpus; genetic markers; genome reannotation; long noncoding RNAs; Saccharina japonica; stramenopile	SMALL NUCLEOLAR RNAS; COMPLEX MULTICELLULARITY; SEQ DATA; SILICULOSUS; REVEALS; EVOLUTION; QUANTIFICATION; TRANSCRIPTOME; GENERATION; EXPRESSION	The genome of the filamentous brown alga Ectocarpus was the first to be completely sequenced from within the brown algal group and has served as a key reference genome both for this lineage and for the stramenopiles. We present a complete structural and functional reannotation of the Ectocarpus genome. The large-scale assembly of the Ectocarpus genome was significantly improved and genome-wide gene re-annotation using extensive RNA-seq data improved the structure of 11 108 existing protein-coding genes and added 2030 new loci. A genome-wide analysis of splicing isoforms identified an average of 1.6 transcripts per locus. A large number of previously undescribed noncoding genes were identified and annotated, including 717 loci that produce long noncoding RNAs. Conservation of lncRNAs between Ectocarpus and another brown alga, the kelp Saccharina japonica, suggests that at least a proportion of these loci serve a function. Finally, a large collection of single nucleotide polymorphism-based markers was developed for genetic analyses. These resources are available through an updated and improved genome database. This study significantly improves the utility of the Ectocarpus genome as a high-quality reference for the study of many important aspects of brown algal biology and as a reference for genomic analyses across the stramenopiles.	[Cormier, Alexandre; Avia, Komlan; Godfroy, Olivier; Lipinska, Agnieszka; Perrineau, Marie-Mathilde; Coelho, Susana M.; Cock, J. Mark] UPMC Univ Paris 06, CNRS, Algal Genet Grp,Stn Biol Roscoff, Integrat Biol Marine Models,UMR 8227,Sorbonne Uni, CS 90074, F-29688 Roscoff, France; [Sterck, Lieven; Van De Peer, Yves] VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium; [Sterck, Lieven; Van De Peer, Yves] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9000 Ghent, Belgium; [Sterck, Lieven; Van De Peer, Yves] Bioinformat Inst Ghent, Technol Pk 927, B-9052 Ghent, Belgium; [Derrien, Thomas; Wucher, Valentin; Hitte, Christophe] Univ Rennes 1, IGDR CNRS, UMR6290, Rennes, France; [Andres, Gwendoline; Corre, Erwan] UPMC, CNRS, Abims Platform, FR2424,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; [Van De Peer, Yves] Univ Pretoria, Genom Res Inst, Dept Genet, ZA-0028 Pretoria, South Africa	Cock, JM (corresponding author), UPMC Univ Paris 06, CNRS, Algal Genet Grp,Stn Biol Roscoff, Integrat Biol Marine Models,UMR 8227,Sorbonne Uni, CS 90074, F-29688 Roscoff, France.	cock@sb-roscoff.fr	Van de Peer, Yves/AAE-7666-2019; Perrineau, Marie-Mathilde/J-4459-2014; Van de Peer, Yves/D-4388-2009; corre, erwan/O-4669-2019; Sterck, Lieven/A-9439-2016; Derrien, Thomas/ABD-6197-2020; Avia, Komlan/E-6850-2015; Coelho, Susana/ABH-8166-2020	Van de Peer, Yves/0000-0003-4327-3730; Perrineau, Marie-Mathilde/0000-0002-1772-7009; Van de Peer, Yves/0000-0003-4327-3730; corre, erwan/0000-0001-6354-2278; Sterck, Lieven/0000-0001-7116-4000; Avia, Komlan/0000-0001-6212-6774; Cormier, Alexandre/0000-0002-7775-8413; Derrien, Thomas/0000-0002-8211-5789; Cock, J. Mark/0000-0002-2650-0383; Wucher, Valentin/0000-0002-1331-4549	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [ANR-10-BLAN-1727, ANR-10-BTBR-04-01, ANR-12JSV7-0008]; University Pierre et Marie Curie; European Research CouncilEuropean Research Council (ERC)European Commission [638240]; Brittany RegionRegion Bretagne	We thank Toshiki Uji for providing RNA-seq data, various members of the Ectocarpus Genome Consortium for manual annotation of genes through the Orcae database and an anonymous reviewer for comments that led to significant improvement of the manuscript. This work was supported by the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (project Bi-cycle ANR-10-BLAN-1727, project Idealg ANR- 10-BTBR-04-01 and project Sexseaweed ANR-12JSV7-0008), the University Pierre et Marie Curie and the European Research Council (grant agreement 638240). A. C. was supported by a grant from the Brittany Region.	Abeel T, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gkr995; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Bratkovic T, 2014, BBA-GENE REGUL MECH, V1839, P438, DOI 10.1016/j.bbagrm.2014.04.009; Buels R, 2016, GENOME BIOL, V17, DOI 10.1186/s13059-016-0924-1; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chekanova JA, 2015, CURR OPIN PLANT BIOL, V27, P207, DOI 10.1016/j.pbi.2015.08.003; Chen L, 2014, MOL BIOL EVOL, V31, P1402, DOI 10.1093/molbev/msu083; Cock JM, 2015, ADV MAR GENOMICS, V2, P335, DOI 10.1007/978-94-017-9642-2_16; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Conesa A, 2005, BIOINFORMATICS, V21, P3674, DOI 10.1093/bioinformatics/bti610; DAYTON PK, 1985, ANNU REV ECOL SYST, V16, P215, DOI 10.1146/annurev.es.16.110185.001243; Degroeve S, 2005, BIOINFORMATICS, V21, P1332, DOI 10.1093/bioinformatics/bti166; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Duret L, 1996, COMPUT APPL BIOSCI, V12, P507; Foissac S, 2008, CURR BIOINFORM, V3, P87, DOI 10.2174/157489308784340702; Godfroy O, 2015, MAR GENOM, V24, P109, DOI 10.1016/j.margen.2015.03.007; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Gremme G, 2005, INFORM SOFTWARE TECH, V47, P965, DOI 10.1016/j.infsof.2005.09.005; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Haas BJ, 2013, NAT PROTOC, V8, P1494, DOI 10.1038/nprot.2013.084; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hoeppner MP, 2012, BMC EVOL BIOL, V12, DOI 10.1186/1471-2148-12-183; Hughes AD, 2012, BIOTECHNOL BIOFUELS, V5, DOI 10.1186/1754-6834-5-86; Jones P, 2014, BIOINFORMATICS, V30, P1236, DOI 10.1093/bioinformatics/btu031; Kahles A, 2016, BIOINFORMATICS, V32, P1840, DOI 10.1093/bioinformatics/btw076; Kawai H, 2015, J PHYCOL, V51, P918, DOI 10.1111/jpy.12332; Kehr S, 2014, MOL BIOL EVOL, V31, P455, DOI 10.1093/molbev/mst209; Kianianmomeni A, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-1117; Kijjoa A., 2004, MAR DRUGS, V2, P73, DOI [DOI 10.3390/MD202073, 10.3390/md202073]; Kim D, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-4-r36; Klinger T, 2015, PERSPECTIVES PHYCOLO, P31, DOI DOI 10.1127/PIP/2015/0024; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Lee Y, 2015, ANNU REV BIOCHEM, V84, P291, DOI 10.1146/annurev-biochem-060614-034316; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Lipinska AP, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-909; Luthringer R, 2015, MOL BIOL EVOL, V32, P2973, DOI 10.1093/molbev/msv173; Meslet-Cladiere L, 2013, PLANT CELL, V25, P3089, DOI 10.1105/tpc.113.111336; MULLER DG, 1966, PLANTA, V68, P57, DOI 10.1007/BF00385371; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Mungall CJ, 2007, BIOINFORMATICS, V23, pI337, DOI 10.1093/bioinformatics/btm189; Pertea M, 2015, NAT BIOTECHNOL, V33, P290, DOI 10.1038/nbt.3122; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; Reddy ASN, 2013, PLANT CELL, V25, P3657, DOI 10.1105/tpc.113.117523; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Smit AJ, 2004, J APPL PHYCOL, V16, P245, DOI 10.1023/B:JAPH.0000047783.36600.ef; Standage DS, 2012, BMC BIOINFORMATICS, V13, DOI 10.1186/1471-2105-13-187; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Tarver JE, 2015, NUCLEIC ACIDS RES, V43, P6384, DOI 10.1093/nar/gkv578; Trapnell C, 2010, NAT BIOTECHNOL, V28, P511, DOI 10.1038/nbt.1621; Tseng CK, 2001, J APPL PHYCOL, V13, P375, DOI 10.1023/A:1017972812576; Ulitsky I, 2013, CELL, V154, P26, DOI 10.1016/j.cell.2013.06.020; WAHL M, 2015, PERSPECT PHYCOL, V2, P11, DOI DOI 10.1127/PIP/2015/0019; Wu XM, 2013, GENOME BIOL EVOL, V5, P1731, DOI 10.1093/gbe/evt115; Yandell M, 2012, NAT REV GENET, V13, P329, DOI 10.1038/nrg3174; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zambounis A, 2012, MOL BIOL EVOL, V29, P1263, DOI 10.1093/molbev/msr296; Zhang RX, 2015, NEW PHYTOL, V208, P96, DOI 10.1111/nph.13545	67	30	30	2	31	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X	1469-8137		NEW PHYTOL	New Phytol.	APR	2017	214	1					219	232		10.1111/nph.14321			14	Plant Sciences	Plant Sciences	EQ5NS	WOS:000398130300022	27870061				2021-04-07	
J	Avia, K; Coelho, SM; Montecinos, GJ; Cormier, A; Lerck, F; Mauger, S; Faugeron, S; Valero, M; Cock, JM; Boudry, P				Avia, Komlan; Coelho, Susana M.; Montecinos, Gabriel J.; Cormier, Alexandre; Lerck, Fiona; Mauger, Stephane; Faugeron, Sylvain; Valero, Myriam; Cock, J. Mark; Boudry, Pierre			High-density genetic map and identification of QTLs for responses to temperature and salinity stresses in the model brown alga Ectocarpus	SCIENTIFIC REPORTS			English	Article							QUANTITATIVE TRAIT LOCUS; PHENOTYPIC PLASTICITY; RECOMBINATION RATE; MARINE MACROALGAE; OXIDATIVE STRESS; LOCAL ADAPTATION; EVOLUTION; TOLERANCE; SILICULOSUS; POPULATIONS	Deciphering the genetic architecture of adaptation of brown algae to environmental stresses such as temperature and salinity is of evolutionary as well as of practical interest. The filamentous brown alga Ectocarpus sp. is a model for the brown algae and its genome has been sequenced. As sessile organisms, brown algae need to be capable of resisting the various abiotic stressors that act in the intertidal zone (e.g. osmotic pressure, temperature, salinity, UV radiation) and previous studies have shown that an important proportion of the expressed genes is regulated in response to hyposaline, hypersaline or oxidative stress conditions. Using the double digest RAD sequencing method, we constructed a dense genetic map with 3,588 SNP markers and identified 39 QTLs for growth-related traits and their plasticity under different temperature and salinity conditions (tolerance to high temperature and low salinity). GO enrichment tests within QTL intervals highlighted membrane transport processes such as ion transporters. Our study represents a significant step towards deciphering the genetic basis of adaptation of Ectocarpus sp. to stress conditions and provides a substantial resource to the increasing list of tools generated for the species.	[Avia, Komlan; Coelho, Susana M.; Montecinos, Gabriel J.; Cormier, Alexandre; Lerck, Fiona; Cock, J. Mark] UPMC, Sorbonne Univ, CNRS, Algal Genet Grp,UMR 8227,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; [Avia, Komlan; Montecinos, Gabriel J.; Mauger, Stephane; Faugeron, Sylvain; Valero, Myriam] UPMC, Sorbonne Univ, Pontificia Univ Catolica Chile,Stn Biol Roscoff, Univ Austral Chile,CNRS,Evolut Biol & Ecol Algae, CS 90074, F-29688 Roscoff, France; [Montecinos, Gabriel J.; Faugeron, Sylvain] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Conservac Marina, Casilla 114-D, Santiago, Chile; [Montecinos, Gabriel J.; Faugeron, Sylvain] Pontificia Univ Catolica Chile, Fac Ciencias Biol, CeBiB, Casilla 114-D, Santiago, Chile; [Boudry, Pierre] UBO, CNRS, IRD,LEMAR,UMR 6539, Ifremer,Ctr Bretagne ZI Pointe Diable,Lab Sci Env, CS 10070, F-29280 Plouzane, France	Avia, K (corresponding author), UPMC, Sorbonne Univ, CNRS, Algal Genet Grp,UMR 8227,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.; Avia, K (corresponding author), UPMC, Sorbonne Univ, Pontificia Univ Catolica Chile,Stn Biol Roscoff, Univ Austral Chile,CNRS,Evolut Biol & Ecol Algae, CS 90074, F-29688 Roscoff, France.	komlan.avia@sb-roscoff.fr	Avia, Komlan/E-6850-2015; Coelho, Susana/ABH-8166-2020; Valero, Myriam/M-6052-2019; Boudry, Pierre/M-3213-2019; Boudry, Pierre/G-2406-2010	Avia, Komlan/0000-0001-6212-6774; Valero, Myriam/0000-0002-9000-1423; Boudry, Pierre/0000-0002-5150-2276; Mauger, Stephane/0000-0002-8779-1516; Faugeron, Sylvain/0000-0001-7258-5229; Cock, J. Mark/0000-0002-2650-0383; Cormier, Alexandre/0000-0002-7775-8413	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); University Pierre and Marie Curie; Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [Idealg ANR-10-BTBR-04-01, ANR-12-JSV7-0008]; Brittany Region grantRegion Bretagne [SAD 2015-ECTOQTL 9119]; International Research Network "Diversity, Evolution and Biotechnology of Marine Algae" (GDRI) [0803]; European Research Council (ERC)European Research Council (ERC)European Commission [638240]; ANR Equipex sessionFrench National Research Agency (ANR) [ANR-10-EQPX-07-01]; FEDEREuropean Commission; Region Nord-Pas-de-Calais-PicardieRegion Hauts-de-France	This work was supported by the Centre National de la Recherche Scientifique, the University Pierre and Marie Curie, the Agence Nationale de la Recherche (project Idealg ANR-10-BTBR-04-01 and ANR-12-JSV7-0008), the Brittany Region grant SAD 2015-ECTOQTL 9119 to Komlan Avia, the International Research Network "Diversity, Evolution and Biotechnology of Marine Algae" (GDRI No 0803) and the European Research Council (ERC, grant agreement 638240). We are very grateful to Philippe Potin and Monique Ras for their management and support. We are grateful to Akira Peters for providing different strains for our experiments and for his advice. We thank Claire Dagin-Thiebaut, Charlotte Roby and Delphine Scornet for precious advice during experiments. We thank Gildas Le Corguille for bioinformatics support. We thank Ian Probert and Jerome Coudret for technical support. We thank Thomas Broquet for his comments on the paper. We thank the SeaDAS Development Group at NASA GSFC, Greenbelt, Maryland, the Ocean Biology Processing Group(OBPG) at NASA GSFC, Greenbelt, Maryland and Brockmann Consult GmbH, Hamburg, Germany for distributing the SeaDAS software and the MODIS data. We thank the UMR 8199 LIGAN-PM Genomics platform (Lille, France) which belongs to the 'Federation de Recherche' 3508 Labex EGID (European Genomics Institute for Diabetes; ANR-10-LABX-46) and was supported by the ANR Equipex 2010 session (ANR-10-EQPX-07-01; 'LIGAN-PM'). The LIGAN-PM Genomics platform (Lille, France) is also supported by the FEDER and the Region Nord-Pas-de-Calais-Picardie. We thank the anonymous reviewers for their comments that led to significant improvement of the manuscript.	Abasht B, 2006, GENET SEL EVOL, V38, P297, DOI 10.1051/gse:2006005; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Apel K, 2004, ANNU REV PLANT BIOL, V55, P373, DOI 10.1146/annurev.arplant.55.031903.141701; Assmann SM, 2013, INT J PLANT SCI, V174, P3, DOI 10.1086/667798; Baird NA, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003376; Bergmann N, 2010, MOL ECOL, V19, P2870, DOI 10.1111/j.1365-294X.2010.04731.x; Bold H. C., 1978, INTRO ALGAE STRUCTUR; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Broman KW, 2009, STAT BIOL HEALTH, P1, DOI 10.1007/978-0-387-92125-9_1; Broman KW, 2003, BIOINFORMATICS, V19, P889, DOI 10.1093/bioinformatics/btg112; Buckler ES, 2009, SCIENCE, V325, P714, DOI 10.1126/science.1174276; BUSTAMANTE RH, 1995, OECOLOGIA, V102, P189, DOI 10.1007/BF00333251; Catchen J, 2013, MOL ECOL, V22, P3124, DOI 10.1111/mec.12354; Cock JM, 2015, ADV MAR GENOMICS, V2, P335, DOI 10.1007/978-94-017-9642-2_16; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collard BCY, 2005, EUPHYTICA, V142, P169, DOI 10.1007/s10681-005-1681-5; Cordell HJ, 2002, HUM MOL GENET, V11, P2463, DOI 10.1093/hmg/11.20.2463; Cormier A., 2016, NEW PHYTOLOGIST; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; Coyer JA, 2011, MAR GENOM, V4, P41, DOI 10.1016/j.margen.2010.12.003; Davison IR, 1996, J PHYCOL, V32, P197, DOI 10.1111/j.0022-3646.1996.00197.x; DAYTON PK, 1985, ANNU REV ECOL SYST, V16, P215, DOI 10.1146/annurev.es.16.110185.001243; DEVICENTE MC, 1993, GENETICS, V134, P585; Dittami SM, 2016, ISME J, V10, P51, DOI 10.1038/ismej.2015.104; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; EBERT D, 1993, HEREDITY, V70, P335, DOI 10.1038/hdy.1993.48; El-Soda M, 2014, TRENDS PLANT SCI, V19, P390, DOI 10.1016/j.tplants.2014.01.001; Fishman L, 2001, GENETICS, V159, P1701; GILLESPIE JH, 1989, GENETICS, V121, P129; Goecke F, 2010, MAR ECOL PROG SER, V409, P267, DOI 10.3354/meps08607; Gratani L., 2014, ADV BOT, V2014; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hoffmann AA, 1999, TRENDS ECOL EVOL, V14, P96, DOI 10.1016/S0169-5347(99)01595-5; Hollants J, 2013, FEMS MICROBIOL ECOL, V83, P1, DOI 10.1111/j.1574-6941.2012.01446.x; Kawecki TJ, 2004, ECOL LETT, V7, P1225, DOI 10.1111/j.1461-0248.2004.00684.x; Kong A, 2010, NATURE, V467, P1099, DOI 10.1038/nature09525; Lacaze X, 2009, HEREDITY, V102, P163, DOI 10.1038/hdy.2008.76; Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]; Lee CE, 1999, TRENDS ECOL EVOL, V14, P284, DOI 10.1016/S0169-5347(99)01596-7; LEWONTIN RC, 1966, EVOLUTION, V20, P315, DOI 10.1111/j.1558-5646.1966.tb03369.x; Lipinska AP, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140535; Mackay TFC, 2009, NAT REV GENET, V10, P565, DOI 10.1038/nrg2612; McCauley LAR, 2007, PHYCOLOGIA, V46, P429, DOI 10.2216/05-08.1; Menge BA, 2000, J EXP MAR BIOL ECOL, V250, P257, DOI 10.1016/S0022-0981(00)00200-8; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Montecinos A. E., 2016, J PHYCOLOGY; Mota CF, 2015, FUNCT ECOL, V29, P640, DOI 10.1111/1365-2435.12373; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Munns R, 2008, ANNU REV PLANT BIOL, V59, P651, DOI 10.1146/annurev.arplant.59.032607.092911; Nakagawa S, 2010, BIOL REV, V85, P935, DOI 10.1111/j.1469-185X.2010.00141.x; Orr HA, 1998, GENETICS, V149, P2099; Paape T., 2012, GENOME BIOL EVOL; Padilla DK, 2013, ADV MAR BIOL, V65, P67, DOI 10.1016/B978-0-12-410498-3.00002-1; PEDERSEN M, 1973, PHYSIOL PLANTARUM, V28, P101, DOI 10.1111/j.1399-3054.1973.tb01158.x; Pedersen M., 1968, ECTOCARPUS FASCICULA; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; PETERS AF, 1993, MAR BIOL, V115, P143, DOI 10.1007/BF00349396; Peters AF, 2015, CRYPTOGAMIE ALGOL, V36, P3, DOI 10.7872/crya.v36.iss1.2015.3; Peterson BK, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0037135; Phillips PC, 2008, NAT REV GENET, V9, P855, DOI 10.1038/nrg2452; Price AH, 2006, TRENDS PLANT SCI, V11, P213, DOI 10.1016/j.tplants.2006.03.006; Ren ZH, 2005, NAT GENET, V37, P1141, DOI 10.1038/ng1643; Rieseberg LH, 2003, PHILOS T R SOC B, V358, P1141, DOI 10.1098/rstb.2003.1283; RUSSELL G, 1975, ESTUAR COAST MAR SCI, V3, P91, DOI 10.1016/0302-3524(75)90008-0; Sanford E, 2011, ANNU REV MAR SCI, V3, P509, DOI 10.1146/annurev-marine-120709-142756; Sauvage C, 2010, ANIM GENET, V41, P390, DOI 10.1111/j.1365-2052.2009.02018.x; Shan TF, 2015, BMC GENOMICS, V16, DOI 10.1186/s12864-015-2184-y; Smit AJ, 2004, J APPL PHYCOL, V16, P245, DOI 10.1023/B:JAPH.0000047783.36600.ef; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; Tiley GP, 2015, BMC EVOL BIOL, V15, DOI 10.1186/s12862-015-0473-3; Voorrips RE, 2002, J HERED, V93, P77, DOI 10.1093/jhered/93.1.77; Wahid A, 2007, ENVIRON EXP BOT, V61, P199, DOI 10.1016/j.envexpbot.2007.05.011; Wang WX, 2003, PLANTA, V218, P1, DOI 10.1007/s00425-003-1105-5; WARD PJ, 1994, HEREDITY, V72, P574, DOI 10.1038/hdy.1994.79; Wei N, 2013, TRENDS BIOTECHNOL, V31, P70, DOI 10.1016/j.tibtech.2012.10.009; West John A., 1996, Muelleria, V9, P29; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zhang JJ, 2014, BIOINFORMATICS, V30, P614, DOI 10.1093/bioinformatics/btt593; Zhang N, 2015, BMC GENOMICS, V16, DOI 10.1186/s12864-015-1825-5	81	21	21	2	39	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	2045-2322			SCI REP-UK	Sci Rep	MAR 3	2017	7								43241	10.1038/srep43241			15	Multidisciplinary Sciences	Science & Technology - Other Topics	EM4MB	WOS:000395286000001	28256542	DOAJ Gold, Green Published			2021-04-07	
J	Cock, JM; Liu, FL; Duan, DL; Bourdareau, S; Lipinska, AP; Coelho, SM; Tarver, JE				Cock, J. Mark; Liu, Fuli; Duan, Delin; Bourdareau, Simon; Lipinska, Agnieszka P.; Coelho, Susana M.; Tarver, James E.			Rapid Evolution of microRNA Loci in the Brown Algae	GENOME BIOLOGY AND EVOLUTION			English	Article						brown algae; Ectocarpus; heterokonts; microRNA; Saccharina japonica; stramenopiles	SMALL NONCODING RNAS; DICTYOSTELIUM-DISCOIDEUM; COMPLEX MULTICELLULARITY; GENE-EXPRESSION; OFFSET RNAS; MIRNAS; ORIGINS; GENOME; RECOGNITION; ANNOTATION	Stringent searches for microRNAs (miRNAs) have so far only identified these molecules in animals, land plants, chlorophyte green algae, slime molds and brown algae. The identification of miRNAs in brown algae was based on the analysis of a single species, the filamentous brown alga Ectocarpus sp. Here, we have used deep sequencing of small RNAs and a recently published genome sequence to identify miRNAs in a second brown alga, the kelp Saccharina japonica. S. japonica possesses a large number of miRNAs (117) and these miRNAs are highly diverse, falling into 98 different families. Surprisingly, none of the S. japonica miRNAs share significant sequence similarity with the Ectocarpus sp. miRNAs. However, the miRNA repertoires of the two species share a number of structural and genomic features indicating that they were generated by similar evolutionary processes and therefore probably evolved within the context of a common, ancestral miRNA system. This lack of sequence similarity suggests that miRNAs evolve rapidly in the brown algae (the two species are separated by similar to 95 Myr of evolution). The sets of predicted targets of miRNAs in the two species were also very different suggesting that the divergence of the miRNAs may have had significant consequences for miRNA function.	[Cock, J. Mark; Bourdareau, Simon; Lipinska, Agnieszka P.; Coelho, Susana M.] UPMC Univ Paris 06, Sorbonne Univ, Stn Biol Roscoff,CNRS, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, Roscoff, France; [Liu, Fuli] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China; [Duan, Delin] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China; [Duan, Delin] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China; [Tarver, James E.] Sch Earth Sci, Life Sci Bldg,24 Tyndall Ave, Bristol BS8 1TQ, Avon, England	Cock, JM (corresponding author), UPMC Univ Paris 06, Sorbonne Univ, Stn Biol Roscoff,CNRS, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, Roscoff, France.	cock@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020; Duan, Delin/G-9002-2011	Bourdareau, Simon/0000-0001-9150-5327; Cock, J. Mark/0000-0002-2650-0383	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); University Pierre and Marie Curie; European Research Council grant "Sexsea" [638240]; Central Public-interest Scientific Institution Basal Research Fund, CAFS [2016PT03]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31302188, 31272660]; Qingdao National Laboratory for Marine Science and Technology [2015ASKJ02]	We would like to thank Masafumi Nozawa for providing us with information about the Drosophila miRNAs. This work was supported by the Centre National de la Recherche Scientifique, the University Pierre and Marie Curie, the European Research Council grant "Sexsea" (638240), the Central Public-interest Scientific Institution Basal Research Fund, CAFS (NO. 2016PT03), the National Natural Science Foundation of China (31302188, 31272660) and the Qingdao National Laboratory for Marine Science and Technology (2015ASKJ02).	Allen E, 2004, NAT GENET, V36, P1282, DOI 10.1038/ng1478; Andrews S., 2016, FASTQC QUALITY CONTR; Avesson L, 2012, RNA, V18, P1771, DOI 10.1261/rna.033175.112; Axtell MJ, 2011, GENOME BIOL, V12, DOI 10.1186/gb-2011-12-4-221; Bartel DP, 2009, CELL, V136, P215, DOI 10.1016/j.cell.2009.01.002; Berezikov E, 2011, NAT REV GENET, V12, P846, DOI 10.1038/nrg3079; Berezikov E, 2011, GENOME RES, V21, P203, DOI 10.1101/gr.116657.110; Bonnet E, 2010, BIOINFORMATICS, V26, P1566, DOI 10.1093/bioinformatics/btq233; Bortoluzzi S, 2011, TRENDS MOL MED, V17, P473, DOI 10.1016/j.molmed.2011.05.005; Brate J, 2016, 076190 BIORIV; Brodersen P, 2008, SCIENCE, V320, P1185, DOI 10.1126/science.1159151; Campo-Paysaa F, 2011, EVOL DEV, V13, P15, DOI 10.1111/j.1525-142X.2010.00452.x; Carthew RW, 2009, CELL, V136, P642, DOI 10.1016/j.cell.2009.01.035; Cerutti H, 2006, CURR GENET, V50, P81, DOI 10.1007/s00294-006-0078-x; Cock JM, 2015, ADV MAR GENOMICS, V2, P335, DOI 10.1007/978-94-017-9642-2_16; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cormier A, 2017, NEW PHYTOL, V214, P219, DOI 10.1111/nph.14321; Curtis HJ, 2012, WIRES RNA, V3, P617, DOI 10.1002/wrna.1122; De Felippes FF, 2008, RNA, V14, P2455, DOI 10.1261/rna.1149408; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Franco-Zorrilla JM, 2007, NAT GENET, V39, P1033, DOI 10.1038/ng2079; Frank F, 2012, EMBO J, V31, P3588, DOI 10.1038/emboj.2012.204; Frank F, 2010, NATURE, V465, P818, DOI 10.1038/nature09039; Friedlander MR, 2012, NUCLEIC ACIDS RES, V40, P37, DOI 10.1093/nar/gkr688; Fromm B, 2015, ANNU REV GENET, V49, P213, DOI 10.1146/annurev-genet-120213-092023; Ghildiyal M, 2009, NAT REV GENET, V10, P94, DOI 10.1038/nrg2504; Grimson A, 2008, NATURE, V455, P1193, DOI 10.1038/nature07415; Heimberg AM, 2008, P NATL ACAD SCI USA, V105, P2946, DOI 10.1073/pnas.0712259105; Hertel J, 2006, BMC GENOMICS, V7, DOI 10.1186/1471-2164-7-25; Hinas A, 2007, NUCLEIC ACIDS RES, V35, P6714, DOI 10.1093/nar/gkm707; Kawai H, 2015, J PHYCOL, V51, P918, DOI 10.1111/jpy.12332; Kozomara A, 2011, NUCLEIC ACIDS RES, V39, pD152, DOI 10.1093/nar/gkq1027; Langenberger D, 2009, BIOINFORMATICS, V25, P2298, DOI 10.1093/bioinformatics/btp419; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Lee HC, 2010, MOL CELL, V38, P803, DOI 10.1016/j.molcel.2010.04.005; Li AL, 2007, CELL RES, V17, P212, DOI 10.1038/sj.cr.7310113; Li WZ, 2015, NUCLEIC ACIDS RES, V43, pW580, DOI 10.1093/nar/gkv279; Lin SJ, 2015, SCIENCE, V350, P691, DOI 10.1126/science.aad0408; Liu FL, 2015, PLANT CELL ENVIRON, V38, P1357, DOI 10.1111/pce.12484; Lopez-Gomollon S, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-697; Lorenz R, 2011, ALGORITHM MOL BIOL, V6, DOI 10.1186/1748-7188-6-26; Lu J, 2008, NAT GENET, V40, P351, DOI 10.1038/ng.73; Luo RB, 2012, GIGASCIENCE, V1, DOI 10.1186/2047-217X-1-18; Marco A, 2012, RNA BIOL, V9, P242, DOI 10.4161/rna.19160; Martin M, 2011, EMBNET J, V17; Mattick JS, 2004, NAT REV GENET, V5, P316, DOI 10.1038/nrg1321; Molnar A, 2007, NATURE, V447, P1126, DOI 10.1038/nature05903; Mukherjee K, 2013, MOL BIOL EVOL, V30, P627, DOI 10.1093/molbev/mss263; Nozawa M, 2012, GENOME BIOL EVOL, V4, P230, DOI 10.1093/gbe/evs002; Nozawa M, 2010, GENOME BIOL EVOL, V2, P180, DOI 10.1093/gbe/evq009; Peterson KJ, 2009, BIOESSAYS, V31, P736, DOI 10.1002/bies.200900033; Piriyapongsa J, 2008, RNA, V14, P814, DOI 10.1261/rna.916708; Piriyapongsa J, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000203; Rajagopalan R, 2006, GENE DEV, V20, P3407, DOI 10.1101/gad.1476406; Rogato A, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-698; Shi WY, 2009, NAT STRUCT MOL BIOL, V16, P183, DOI 10.1038/nsmb.1536; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Sperling EA, 2010, GEOBIOLOGY, V8, P24, DOI 10.1111/j.1472-4669.2009.00225.x; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tarver JE, 2015, NUCLEIC ACIDS RES, V43, P6384, DOI 10.1093/nar/gkv578; Tarver JE, 2013, MOL BIOL EVOL, V30, P2369, DOI 10.1093/molbev/mst133; Tarver JE, 2012, BIOESSAYS, V34, P857, DOI 10.1002/bies.201200055; Taylor RS, 2014, TRENDS PLANT SCI, V19, P175, DOI 10.1016/j.tplants.2013.11.008; Yang XZ, 2011, BIOINFORMATICS, V27, P2614, DOI 10.1093/bioinformatics/btr430; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zhang WX, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-8-r81; Zhao T, 2007, GENE DEV, V21, P1190, DOI 10.1101/gad.1543507	67	11	11	2	13	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	1759-6653			GENOME BIOL EVOL	Genome Biol. Evol.	MAR	2017	9	3					740	749		10.1093/gbe/evx038			10	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	EV9HC	WOS:000402095700026	28338896	DOAJ Gold, Green Published			2021-04-07	
J	Muyle, A; Shearn, R; Marais, GAB				Muyle, Aline; Shearn, Rylan; Marais, Gabriel A. B.			The Evolution of Sex Chromosomes and Dosage Compensation in Plants	GENOME BIOLOGY AND EVOLUTION			English	Article						Y degeneration; dioecy; sex chromosome turnover; sex-biased expression; sex chromosome sequencing	BIASED GENE-EXPRESSION; PALM PHOENIX-DACTYLIFERA; LATIFOLIA Y-CHROMOSOME; BROWN ALGA ECTOCARPUS; SILENE-LATIFOLIA; X-CHROMOSOME; DIOECIOUS PLANT; DATE PALM; FLOWERING PLANTS; GENOME STRUCTURE	Plant sex chromosomes can be vastly different from those of the few historical animal model organisms from which most of our understanding of sex chromosome evolution is derived. Recently, we have seen several advancements from studies on green algae, brown algae, and land plants that are providing a broader understanding of the variable ways in which sex chromosomes can evolve in distant eukaryotic groups. Plant sex-determining genes are being identified and, as expected, are completely different from those in animals. Species with varying levels of differentiation between the X and Y have been found in plants, and these are hypothesized to be representing different stages of sex chromosome evolution. However, we are also finding that sex chromosomes can remain morphologically unchanged over extended periods of time. Where degeneration of the Y occurs, it appears to proceed similarly in plants and animals. Dosage compensation (a phenomenon that compensates for the consequent loss of expression from the Y) has now been documented in a plant system, its mechanism, however, remains unknown. Research has also begun on the role of sex chromosomes in sexual conflict resolution, and it appears that sex-biased genes evolve similarly in plants and animals, although the functions of these genes remain poorly studied. Because the difficulty in obtaining sex chromosome sequences is increasingly being overcome by methodological developments, there is great potential for further discovery within the field of plant sex chromosome evolution.	[Muyle, Aline; Shearn, Rylan; Marais, Gabriel A. B.] Univ Lyon 1, UMR 5558, Lab Biometrie & Biol Evolutive, CNRS, Villeurbanne, France	Muyle, A (corresponding author), Univ Lyon 1, UMR 5558, Lab Biometrie & Biol Evolutive, CNRS, Villeurbanne, France.	aline.muyle@univ-lyon1.fr			ANRFrench National Research Agency (ANR) [ANR-12-BSV7-0002-04, ANR-14-CE19-0021-01]	We thank two anonymous referees, Susanne Renner and editor Kateryna Makova for their helpful comments, Jeff Lemaitre for helpful discussions about generation times, and Benjamin Herran for preparing a previous version of figure 3. This work was supported by ANR (grant numbers: ANR-12-BSV7-0002-04 and ANR-14-CE19-0021-01).	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Akagi T, 2014, SCIENCE, V346, P646, DOI 10.1126/science.1257225; Al-Dous EK, 2011, NAT BIOTECHNOL, V29, P521, DOI 10.1038/nbt.1860; Al-Mssallem IS, 2013, NAT COMMUN, V4, DOI 10.1038/ncomms3274; Albritton SE, 2014, GENETICS, V197, P865, DOI 10.1534/genetics.114.163311; Bachtrog D, 2006, CURR OPIN GENET DEV, V16, P578, DOI 10.1016/j.gde.2006.10.007; Bachtrog D, 2013, NAT REV GENET, V14, P113, DOI 10.1038/nrg3366; Bachtrog D, 2011, CURR BIOL, V21, pR685, DOI 10.1016/j.cub.2011.08.027; Barrett SCH, 2013, J EXP BOT, V64, P67, DOI 10.1093/jxb/ers308; Bergero R, 2008, GENETICS, V178, P2045, DOI 10.1534/genetics.107.084566; Bergero R, 2007, GENETICS, V175, P1945, DOI 10.1534/genetics.106.070110; Bergero R, 2015, CURR BIOL, V25, P1234, DOI 10.1016/j.cub.2015.03.015; Bergero R, 2013, GENETICS, V194, P673, DOI 10.1534/genetics.113.150755; Bergero R, 2011, CURR BIOL, V21, P1470, DOI 10.1016/j.cub.2011.07.032; Bergero R, 2009, TRENDS ECOL EVOL, V24, P94, DOI 10.1016/j.tree.2008.09.010; Bernasconi G, 2009, HEREDITY, V103, P5, DOI 10.1038/hdy.2009.34; Betran E, 2004, CELL CYCLE, V3, P873; Blaser O, 2014, AM NAT, V183, P140, DOI 10.1086/674026; Blaser O, 2013, EVOLUTION, V67, P635, DOI 10.1111/j.1558-5646.2012.01810.x; Blavet N, 2015, BMC GENOMICS, V16, DOI 10.1186/s12864-015-1698-7; Boualem A, 2015, SCIENCE, V350, P688, DOI 10.1126/science.aac8370; Boualem A, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0006144; Boualem K, 2008, RES MICROBIOL, V159, P110, DOI 10.1016/j.resmic.2007.10.004; Carvalho AB, 2013, GENOME RES, V23, P1894, DOI 10.1101/gr.156034.113; Carvalho FA, 2012, MOL PHYLOGENET EVOL, V65, P46, DOI 10.1016/j.ympev.2012.05.019; CHARLESWORTH B, 1978, AM NAT, V112, P975, DOI 10.1086/283342; Charlesworth B, 2000, PHILOS T R SOC B, V355, P1563, DOI 10.1098/rstb.2000.0717; Charlesworth B, 1996, CURR BIOL, V6, P149, DOI 10.1016/S0960-9822(02)00448-7; Charlesworth B, 2009, NAT REV GENET, V10, P195, DOI 10.1038/nrg2526; CHARLESWORTH D, 1980, GENET RES, V35, P205, DOI 10.1017/S0016672300014051; Charlesworth D, 2016, ANNU REV PLANT BIOL, V67, P397, DOI 10.1146/annurev-arplant-043015-111911; Charlesworth D, 2015, NEW PHYTOL, V208, P52, DOI 10.1111/nph.13497; Chen XS, 2015, MOL BIOL EVOL, V32, P1456, DOI 10.1093/molbev/msv036; Chen YA, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0147671; Cherif E, 2013, NEW PHYTOL, V197, P409, DOI 10.1111/nph.12069; Chibalina MV, 2011, CURR BIOL, V21, P1475, DOI 10.1016/j.cub.2011.07.045; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cortez D, 2014, NATURE, V508, P488, DOI 10.1038/nature13151; Dai XG, 2014, CELL RES, V24, P1274, DOI 10.1038/cr.2014.83; Disteche CM, 2012, ANNU REV GENET, V46, P537, DOI 10.1146/annurev-genet-110711-155454; Dooner HK, 2008, PLANT CELL, V20, P249, DOI 10.1105/tpc.107.057596; Ellegren H, 2007, NAT REV GENET, V8, P689, DOI 10.1038/nrg2167; Ellegren H, 2011, NAT REV GENET, V12, P157, DOI 10.1038/nrg2948; Endress PK, 2015, TAXON, V64, P1093, DOI 10.12705/646.1; Ercan Sevinc, 2015, J Genomics, V3, P1, DOI 10.7150/jgen.10404; Ferris P, 2010, SCIENCE, V328, P351, DOI 10.1126/science.1186222; Ferris PJ, 1997, GENETICS, V146, P859; Filatov DA, 2005, MOL BIOL EVOL, V22, P402, DOI 10.1093/molbev/msi003; Gautier M, 2014, MOL ECOL RESOUR, V14, P1141, DOI 10.1111/1755-0998.12264; Geng S, 2014, PLOS BIOL, V12, DOI 10.1371/journal.pbio.1001904; Geraldes A, 2015, MOL ECOL, V24, P3243, DOI 10.1111/mec.13126; Graves JAM, 2016, NAT REV GENET, V17, P33, DOI 10.1038/nrg.2015.2; Gschwend AR, 2012, P NATL ACAD SCI USA, V109, P13716, DOI 10.1073/pnas.1121096109; Harkess A, 2015, NEW PHYTOL, V207, P883, DOI 10.1111/nph.13389; Honys D, 2004, GENOME BIOL, V5, DOI 10.1186/gb-2004-5-11-r85; Hou J, 2015, SCI REP-UK, V5, DOI 10.1038/srep09076; Hough J, 2014, P NATL ACAD SCI USA, V111, P7713, DOI 10.1073/pnas.1319227111; Howell EC, 2009, GENETICS, V182, P1109, DOI 10.1534/genetics.109.103580; Hughes JF, 2012, ANNU REV GENOM HUM G, V13, P83, DOI 10.1146/annurev-genom-090711-163855; Hughes JF, 2012, NATURE, V483, P82, DOI 10.1038/nature10843; Hughes JF, 2010, NATURE, V463, P536, DOI 10.1038/nature08700; Hurst LD, 2015, PLOS BIOL, V13, DOI 10.1371/journal.pbio.1002315; Jiang H, 2012, J EXP BOT, V63, P3709, DOI 10.1093/jxb/ers064; Jordan CY, 2012, EVOLUTION, V66, P505, DOI 10.1111/j.1558-5646.2011.01448.x; Julien P, 2012, PLOS BIOL, V10, DOI 10.1371/journal.pbio.1001328; Kafer J, 2014, J EVOLUTION BIOL, V27, P1478, DOI 10.1111/jeb.12385; Kazama Y, 2016, SCI REP-UK, V6, DOI 10.1038/srep18917; Kazama Y, 2012, G3-GENES GENOM GENET, V2, P1269, DOI 10.1534/g3.112.003749; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Malone JH, 2012, GENOME BIOL, V13, DOI 10.1186/gb-2012-13-4-r28; Manchester SR, 2006, INT J PLANT SCI, V167, P897, DOI 10.1086/503918; Mank JE, 2013, TRENDS GENET, V29, P677, DOI 10.1016/j.tig.2013.07.005; Martin A, 2009, NATURE, V461, P1135, DOI 10.1038/nature08498; Martins MJF, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-294; Mathew LS, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-285; Matsunaga Sachihiro, 1994, Cytologia (Tokyo), V59, P135, DOI 10.1508/cytologia.59.135; McDaniel SF, 2007, GENETICS, V176, P2489, DOI 10.1534/genetics.107.075424; McDaniel SF, 2013, EVOLUTION, V67, P2811, DOI 10.1111/evo.12165; Michalovova M, 2015, BMC BIOINFORMATICS, V16, DOI 10.1186/s12859-015-0509-0; Ming R, 2008, NATURE, V452, P991, DOI 10.1038/nature06856; Ming R, 2011, ANNU REV PLANT BIOL, V62, P485, DOI 10.1146/annurev-arplant-042110-103914; Mullon C, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms8720; Muyle A, 2016, GENOME BIOL EVOL, V8, P2530, DOI 10.1093/gbe/evw172; Muyle A, 2012, PLOS BIOL, V10, DOI 10.1371/journal.pbio.1001308; Navajas-Perez R, 2005, MOL BIOL EVOL, V22, P1929, DOI 10.1093/molbev/msi186; Nicolas M, 2005, PLOS BIOL, V3, P47, DOI 10.1371/journal.pbio.0030004; Ohno S., 1967, SEX CHROMOSOMES SEX, V1, P192; Okada S, 2001, P NATL ACAD SCI USA, V98, P9454, DOI 10.1073/pnas.171304798; Papadopulos AST, 2015, P NATL ACAD SCI USA, V112, P13021, DOI 10.1073/pnas.1508454112; Perrin N, 2009, EVOLUTION, V63, P3043, DOI 10.1111/j.1558-5646.2009.00837.x; Pessia E, 2014, CELL MOL LIFE SCI, V71, P1383, DOI 10.1007/s00018-013-1499-6; Pessia E, 2012, P NATL ACAD SCI USA, V109, P5346, DOI 10.1073/pnas.1116763109; Picq S, 2014, BMC PLANT BIOL, V14, DOI 10.1186/s12870-014-0229-z; Prochnik SE, 2010, SCIENCE, V329, P223, DOI 10.1126/science.1188800; Pucholt P, 2015, HEREDITY, V114, P575, DOI 10.1038/hdy.2014.125; Qiu S, 2013, GENETICS, V194, P663, DOI 10.1534/genetics.113.152397; Rautenberg A, 2010, MOL PHYLOGENET EVOL, V57, P978, DOI 10.1016/j.ympev.2010.08.003; Renner SS, 2016, AM J BOT, V103, P587, DOI 10.3732/ajb.1600029; Renner SS, 2014, AM J BOT, V101, P1588, DOI 10.3732/ajb.1400196; RICE WR, 1984, EVOLUTION, V38, P735, DOI [10.2307/2408385, 10.1111/j.1558-5646.1984.tb00346.x]; RICE WR, 1987, EVOLUTION, V41, P911, DOI 10.1111/j.1558-5646.1987.tb05864.x; Robinson KM, 2014, BMC PLANT BIOL, V14, DOI 10.1186/s12870-014-0276-5; Ross MT, 2005, NATURE, V434, P325, DOI 10.1038/nature03440; Schafer M, 1995, DEV BIOL, V172, P344, DOI 10.1006/dbio.1995.8049; SEGAWA M, 1971, JPN J GENET, V46, P33, DOI 10.1266/jjg.46.33; Skaletsky H, 2003, NATURE, V423, P825, DOI 10.1038/nature01722; Slancarova V, 2013, EVOLUTION, V67, P3669, DOI 10.1111/evo.12223; SMITH BW, 1964, EVOLUTION, V18, P93, DOI 10.1111/j.1558-5646.1964.tb01573.x; Sousa A, 2013, CYTOGENET GENOME RES, V139, P107, DOI 10.1159/000345370; Sousa A, 2016, PLANT J, V88, P387, DOI 10.1111/tpj.13254; Spigler RB, 2008, HEREDITY, V101, P507, DOI 10.1038/hdy.2008.100; Steflova P, 2013, GENOME BIOL EVOL, V5, P769, DOI 10.1093/gbe/evt049; STETTLER R F, 1971, Silvae Genetica, V20, P42; Telgmann-Rauber A, 2007, MOL GENET GENOMICS, V278, P221, DOI 10.1007/s00438-007-0235-z; Toups M, 2015, CURR BIOL, V25, pR427, DOI 10.1016/j.cub.2015.03.054; Tuskan GA, 2012, TREE GENET GENOMES, V8, P559, DOI 10.1007/s11295-012-0495-6; van Doorn GS, 2007, NATURE, V449, P909, DOI 10.1038/nature06178; van Doorn GS, 2010, GENETICS, V186, P629, DOI 10.1534/genetics.110.118596; VanBuren R, 2015, GENOME RES, V25, P524, DOI 10.1101/gr.183905.114; Veitia RA, 2015, J MOL CELL BIOL, V7, P2, DOI 10.1093/jmcb/mjv001; Vicoso B, 2013, PLOS BIOL, V11, DOI 10.1371/journal.pbio.1001643; Vicoso B, 2013, P NATL ACAD SCI USA, V110, P6453, DOI 10.1073/pnas.1217027110; Vicoso B, 2011, GENOME BIOL EVOL, V3, P230, DOI 10.1093/gbe/evr010; Vyskot B, 2015, PLANT SCI, V236, P126, DOI 10.1016/j.plantsci.2015.03.019; Wang JP, 2012, P NATL ACAD SCI USA, V109, P13710, DOI 10.1073/pnas.1207833109; Wang X, 2014, HEREDITY, V113, P156, DOI 10.1038/hdy.2014.18; Xiong YY, 2010, NAT GENET, V42, P1043, DOI 10.1038/ng.711; Yamato KT, 2007, P NATL ACAD SCI USA, V104, P6472, DOI 10.1073/pnas.0609054104; Yin T, 2008, GENOME RES, V18, P422, DOI 10.1101/gr.7076308; Zemp N, 2016, NAT PLANTS, V2, DOI [10.1038/NPLANTS.2016.168, 10.1038/nplants.2016.168]; Zhou Q, 2012, SCIENCE, V337, P341, DOI 10.1126/science.1225385; Zluvova J, 2007, GENETICS, V177, P375, DOI 10.1534/genetics.107.071175	132	28	28	1	20	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	1759-6653			GENOME BIOL EVOL	Genome Biol. Evol.	MAR	2017	9	3					627	645		10.1093/gbe/evw282			19	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	EV9HC	WOS:000402095700019	28391324	DOAJ Gold, Green Published			2021-04-07	
J	Yang, QF; Liu, L; Liu, Y; Zhou, ZG				Yang, Qi-Fan; Liu, Li; Liu, Yu; Zhou, Zhi-Gang			TELOMERIC LOCALIZATION OF THE ARABIDOPSIS-TYPE HEPTAMER REPEAT, (TTTAGGG)(N), AT THE CHROMOSOME ENDS IN SACCHARINA JAPONICA (PHAEOPHYTA)	JOURNAL OF PHYCOLOGY			English	Article						Bal31; chromosome; FISH; kelp; Laminaria japonica; Southern blot; telomeric repeat	IN-SITU HYBRIDIZATION; HIGHER-PLANTS; BROWN-ALGAE; SEQUENCES; EVOLUTION; GENOME; ORGANIZATION; ABSENCE; CLONING; DNA	Telomeres generally consist of short repeats of minisatellite DNA sequences and are useful in chromosome identification and karyotype analysis. To date, telomeres have not been characterized in the economically important brown seaweed Saccharina japonica, thus its full cytogenetic research and genetic breeding potential has not been realized. Herein, the tentative sequence of telomeres in S. japonica was identified by PCR amplification with primers designed based on the Arabidopsis-type telomere sequence (TTTAGGG)(n), which was chosen out of three possible telomeric repeat DNA sequences typically present in plants and algae. After PCR optimization and cloning, sequence analysis of the amplified products from S. japonica genomic DNA showed that they were composed of repeat units, (TTTAGGG)(n), in which the repeat number ranged from 15 to 63 (n = 46). This type of repeat sequence was verified by a Southern blot assay with the Arabidopsis-type telomere sequence as a probe. The digestion of S. japonica genomic DNA with the exonuclease Bal31 illustrated that the target sequence corresponding to the Arabidopsis-type telomere sequence was susceptible to Bal31 digestion, suggesting that the repeat sequence was likely located at the outermost ends of the kelp chromosomes. Fluorescence in situ hybridizations with the aforementioned probe provided the initial cytogenetic evidence that the hybridization signals were principally localized at both ends of S. japonica chromosomes. This study indicates that the telomeric repeat of the kelp chromosomes is (TTTAGGG)(n) which differs from the previously reported (TTAGGG)(n) sequence in Ectocarpus siliculosus through genome sequencing, thereby suggesting distinct telomeres in brown seaweeds.	[Yang, Qi-Fan; Liu, Li; Liu, Yu; Zhou, Zhi-Gang] Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai 201306, Peoples R China	Zhou, ZG (corresponding author), Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai 201306, Peoples R China.	zgzhou@shou.edu.cn	ZHOU, Zhi-Gang/V-9675-2019		National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41376136]; Shanghai Universities Plateau Discipline Project of Marine Sciences	This research was supported by the National Natural Science Foundation of China (grant no. 41376136) and the Shanghai Universities Plateau Discipline Project of Marine Sciences.	Adams SP, 2000, CHROMOSOMA, V109, P201, DOI 10.1007/s004120050429; BLACKBURN EH, 1978, J MOL BIOL, V120, P33, DOI 10.1016/0022-2836(78)90294-2; Blanc G, 2010, PLANT CELL, V22, P2943, DOI 10.1105/tpc.110.076406; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; EVANS LV, 1963, NATURE, V198, P215, DOI 10.1038/198215a0; EVANS LV, 1965, ANN BOT-LONDON, V29, P541, DOI 10.1093/oxfordjournals.aob.a083971; FAJKUS J, 1995, MOL GEN GENET, V247, P633, DOI 10.1007/BF00290355; Fajkus P, 2016, PLANT J, V85, P337, DOI 10.1111/tpj.13115; Fojtova M, 2010, CHROMOSOMA, V119, P485, DOI 10.1007/s00412-010-0272-y; FUCHS J, 1995, PLANT SYST EVOL, V196, P227, DOI 10.1007/BF00982962; Fulneckova J, 2013, GENOME BIOL EVOL, V5, P468, DOI 10.1093/gbe/evt019; Fulneckova J, 2012, GENOME BIOL EVOL, V4, P248, DOI 10.1093/gbe/evs007; GANAL MW, 1991, PLANT CELL, V3, P87, DOI 10.1105/tpc.3.1.87; HIGASHIYAMA T, 1995, MOL GEN GENET, V246, P29, DOI 10.1007/BF00290130; Hu YJ, 2001, J APPL PHYCOL, V13, P415, DOI 10.1023/A:1011920213639; Jiang JM, 1996, MOL GEN GENET, V252, P497, DOI 10.1007/BF02172395; KILIAN A, 1992, MOL GEN GENET, V235, P153, DOI 10.1007/BF00286193; Lewis RJ, 1996, PHYCOLOGIA, V35, P19, DOI 10.2216/i0031-8884-35-1-19.1; Lim KB, 2001, GENOME, V44, P911, DOI 10.1139/gen-44-5-911; Lin KW, 2008, MUTAT RES-REV MUTAT, V658, P95, DOI 10.1016/j.mrrev.2007.08.006; Ling J, 2012, J INTEGR AGR, V11, P1417, DOI 10.1016/S2095-3119(12)60141-5; Liu Y, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0031406; McClintock B, 1939, P NATL ACAD SCI USA, V25, P405, DOI 10.1073/pnas.25.8.405; McKnight TD, 2004, PLANT CELL, V16, P794, DOI 10.1105/tpc.160470; MOYZIS RK, 1988, P NATL ACAD SCI USA, V85, P6622, DOI 10.1073/pnas.85.18.6622; PETRACEK ME, 1990, P NATL ACAD SCI USA, V87, P8222, DOI 10.1073/pnas.87.21.8222; Pich U, 1996, CHROMOSOME RES, V4, P207, DOI 10.1007/BF02254961; RICHARDS EJ, 1988, CELL, V53, P127, DOI 10.1016/0092-8674(88)90494-1; Riha K, 2001, SCIENCE, V291, P1797, DOI 10.1126/science.1057110; SCHWEIZER D, 1976, CHROMOSOMA, V58, P307, DOI 10.1007/BF00292840; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Sykorova E, 2003, PLANT J, V34, P283, DOI 10.1046/j.1365-313X.2003.01731.x; YABU H, 1991, Japanese Journal of Phycology, V39, P185; Zhou Z.-G., 1998, CHIN J BIOTECHNOL, V14, P109, DOI DOI 10.13345/J.CJB.1998.01.020	34	3	3	2	13	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	FEB	2017	53	1					235	240		10.1111/jpy.12497			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	EP3CJ	WOS:000397259800020	27885670				2021-04-07	
J	Jia, F; Ben Amar, M; Billoud, B; Charrier, B				Jia, Fei; Ben Amar, Martine; Billoud, Bernard; Charrier, Benedicte			Morphoelasticity in the development of brown alga Ectocarpus siliculosus: from cell rounding to branching	JOURNAL OF THE ROYAL SOCIETY INTERFACE			English	Article						growth; morphogenesis; cell division; seaweed; bioelasticity; poroelasticity	EXTRACELLULAR-MATRIX; TIP GROWTH; MORPHOGENESIS; PHAEOPHYCEAE; MODEL; WALL; EVOLUTION; TRANSPORT; INSIGHTS; SHAPE	A biomechanical model is proposed for the growth of the brown alga Ectocarpus siliculosus. Featuring ramified uniseriate filaments, this alga has two modes of growth: apical growth and intercalary growth with branching. Apical growth occurs upon the mitosis of a young cell at one extremity and leads to a new tip cell followed by a cylindrical cell, whereas branching mainly occurs when a cylindrical cell becomes rounded and swells, forming a spherical cell. Given the continuous interplay between cell growth and swelling, a poroelastic model combining osmotic pressure and volumetric growth is considered for the whole cell, cytoplasm and cell wall. The model recovers the morphogenetic transformations of mature cells: transformation of a cylindrical shape into spherical shape with a volumetric increase, and then lateral branching. Our simulations show that the poroelastic model, including the Mooney-Rivlin approach for hyper-elastic materials, can correctly reproduce the observations. In particular, branching appears to be a plasticity effect due to the high level of tension created after the increase in volume of mature cells.	[Jia, Fei] Southwest Univ Sci & Technol, Sch Mfg Sci & Engn, Mianyang 621010, Sichuan, Peoples R China; [Ben Amar, Martine] UPMC Univ Paris 06, Sorbonne Univ, PSL Res Univ, Lab Phys Stat,Ecole Normale Super,CNRS, 24 Rue Lhomond, F-75005 Paris, France; [Ben Amar, Martine] Univ Pierre & Marie Curie Paris 6, Inst Univ Cancerol, Fac Med, 91 Bd Hop, F-75013 Paris, France; [Billoud, Bernard; Charrier, Benedicte] UPMC, CNRS, UMR8227, Biol Stn, Pl George Teissier, F-29680 Roscoff, France	Ben Amar, M (corresponding author), UPMC Univ Paris 06, Sorbonne Univ, PSL Res Univ, Lab Phys Stat,Ecole Normale Super,CNRS, 24 Rue Lhomond, F-75005 Paris, France.; Ben Amar, M (corresponding author), Univ Pierre & Marie Curie Paris 6, Inst Univ Cancerol, Fac Med, 91 Bd Hop, F-75013 Paris, France.	benamar@lps.ens.fr		Jia, Fei/0000-0001-7329-9793; Billoud, Bernard/0000-0002-5140-8087; Charrier, Benedicte/0000-0001-5721-1640	Institut Universitaire de France; Reseau Andre Picard for Biology of Development,Sorbonne Universites [ANR11-IDEX-0004-02]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [11402217]	This work is supported in part by the Institut Universitaire de France and the Reseau Andre Picard for Biology of Development, ANR11-IDEX-0004-02, Sorbonne Universites. F.J. acknowledges the support of National Natural Science Foundation of China (grant no. 11402217).	Abenza JF, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms9400; Ateshian GA, 2006, J BIOMECH, V39, P464, DOI 10.1016/j.jbiomech.2004.12.013; Ateshian GA, 2007, BIOMECH MODEL MECHAN, V6, P423, DOI 10.1007/s10237-006-0070-x; Ateshian GA, 2010, BIOMECH MODEL MECHAN, V9, P689, DOI 10.1007/s10237-010-0205-y; Ben Amar M, 2015, J R SOC INTERFACE, V12, DOI 10.1098/rsif.2015.0343; Ben Amar M, 2012, NEW J PHYS, V14, DOI 10.1088/1367-2630/14/8/085014; Billoud B, 2008, FUNCT PLANT BIOL, V35, P1014, DOI 10.1071/FP08036; Campas O, 2012, AM J BOT, V99, P1577, DOI 10.3732/ajb.1200087; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Ciarletta P, 2012, INT J NONLIN MECH, V47, P248, DOI 10.1016/j.ijnonlinmec.2011.05.013; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; De Gennes P.G., 1979, SCALING CONCEPTS POL; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Dervaux J, 2008, PHYS REV LETT, V101, DOI 10.1103/PhysRevLett.101.068101; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dumais J, 2006, INT J DEV BIOL, V50, P209, DOI 10.1387/ijdb.052066jd; Dumais J, 2000, J PLANT GROWTH REGUL, V19, P253, DOI 10.1007/s003440000035; Flory PJ, 1942, J CHEM PHYS, V10, P51, DOI 10.1063/1.1723621; Goriely A, 2005, PHYS REV LETT, V94, DOI 10.1103/PhysRevLett.94.198103; Goriely A, 2003, J THEOR BIOL, V222, P211, DOI 10.1016/S0022-5193(03)00029-8; Goriely A, 2015, BIOMECH MODEL MECHAN, V14, P931, DOI 10.1007/s10237-015-0662-4; Goriely Alain, 2008, Fungal Biology Reviews, V22, P77, DOI 10.1016/j.fbr.2008.05.001; Hamant O, 2008, SCIENCE, V322, P1650, DOI 10.1126/science.1165594; Hamant O, 2010, CURR OPIN GENET DEV, V20, P454, DOI 10.1016/j.gde.2010.04.009; Holzapfel G.A., 2002, NONLINEAR SOLID MECH; Holzapfel GA, 2014, J MECH BEHAV BIOMED, V38, P78, DOI 10.1016/j.jmbbm.2014.05.014; Iwamoto S, 2009, BIOMACROMOLECULES, V10, P2571, DOI 10.1021/bm900520n; Jia F, 2015, PHYS REV E, V91, DOI 10.1103/PhysRevE.91.012403; Jia F, 2014, INT J APPL MECH, V6, DOI 10.1142/S1758825114500033; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; KLOAREG B, 1986, INT J BIOL MACROMOL, V8, P380, DOI 10.1016/0141-8130(86)90060-7; Landau LD, 1990, PHYS THEORIQUE THEOR; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail Aude, 2013, Methods Mol Biol, V959, P323, DOI 10.1007/978-1-62703-221-6_22; Li B, 2011, PHYS REV LETT, V106, DOI 10.1103/PhysRevLett.106.234301; LOCKHART JA, 1965, J THEOR BIOL, V8, P264, DOI 10.1016/0022-5193(65)90077-9; Mauck RL, 2003, J BIOMECH ENG-T ASME, V125, P602, DOI 10.1115/1.1611512; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Minc N, 2014, CURR BIOL, V24, P1436, DOI 10.1016/j.cub.2014.05.054; Mirabet V, 2011, ANNU REV PLANT BIOL, V62, P365, DOI 10.1146/annurev-arplant-042110-103852; Ogden R.W., 1997, NONLINEAR ELASTIC DE; Tallinen T, 2014, P NATL ACAD SCI USA, V111, P12667, DOI 10.1073/pnas.1406015111; Terauchi M, 2016, PLANTA, V244, P361, DOI 10.1007/s00425-016-2516-4; Tesson B, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00471; Turlier H, 2014, BIOPHYS J, V106, P114, DOI 10.1016/j.bpj.2013.11.014; Wu M, 2015, MATH MECH SOLIDS, V20, P663, DOI 10.1177/1081286514550571	46	5	5	2	12	ROYAL SOC	LONDON	6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND	1742-5689	1742-5662		J R SOC INTERFACE	J. R. Soc. Interface	FEB 1	2017	14	127							20160596	10.1098/rsif.2016.0596			10	Multidisciplinary Sciences	Science & Technology - Other Topics	EN4ZT	WOS:000396016100001	28228537	Green Published, Other Gold			2021-04-07	
J	Macaisne, N; Liu, FL; Scornet, D; Peters, AF; Lipinska, A; Perrineau, MM; Henry, A; Strittmatter, M; Coelho, SM; Cock, JM				Macaisne, Nicolas; Liu, Fuli; Scornet, Delphine; Peters, Akira F.; Lipinska, Agnieszka; Perrineau, Marie-Mathilde; Henry, Antoine; Strittmatter, Martina; Coelho, Susana M.; Cock, J. Mark			The Ectocarpus IMMEDIATE UPRIGHT gene encodes a member of a novel family of cysteine-rich proteins with an unusual distribution across the eukaryotes	DEVELOPMENT			English	Article						Brown algae; Ectocarpus; Horizontal gene transfer; Initial cell division; Life cycle; Virus	GENOME REVEALS; LIFE-CYCLES; LAND PLANTS; EVOLUTION; EXPRESSION; SEQUENCE; ALGAE; TRANSITION; PHYLOGENY; ALIGNMENT	The sporophyte generation of the brown alga Ectocarpus sp. exhibits an unusual pattern of development compared with the majority of brown algae. The first cell division is symmetrical and the apical-basal axis is established late in development. In the immediate upright (imm) mutant, the initial cell undergoes an asymmetric division to immediately establish the apical-basal axis. We provide evidence which suggests that this phenotype corresponds to the ancestral state of the sporophyte. The IMM gene encodes a protein of unknown function that contains a repeated motif also found in the EsV-1-7 gene of the Ectocarpus virus EsV-1. Brown algae possess large families of EsV-1-7 domain genes but these genes are rare in other stramenopiles, suggesting that the expansion of this family might have been linked with the emergence of multicellular complexity. EsV-1-7 domain genes have a patchy distribution across eukaryotic supergroups and occur in several viral genomes, suggesting possible horizontal transfer during eukaryote evolution.	[Macaisne, Nicolas; Liu, Fuli; Scornet, Delphine; Lipinska, Agnieszka; Perrineau, Marie-Mathilde; Henry, Antoine; Strittmatter, Martina; Coelho, Susana M.; Cock, J. Mark] Univ Paris 06, Sorbonne Univ, CNRS, Algal Genet Grp,Stn Biol Roscoff,UMR 8227,Integra, Stn Biologique Roscoff,CS 90074, F-29688 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France; [Liu, Fuli] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China	Cock, JM (corresponding author), Univ Paris 06, Sorbonne Univ, CNRS, Algal Genet Grp,Stn Biol Roscoff,UMR 8227,Integra, Stn Biologique Roscoff,CS 90074, F-29688 Roscoff, France.	cock@sb-roscoff.fr	Perrineau, Marie-Mathilde/J-4459-2014; Coelho, Susana/ABH-8166-2020	Perrineau, Marie-Mathilde/0000-0002-1772-7009; Peters, Akira/0000-0001-5332-199X; Strittmatter, Martina/0000-0002-1258-9751; HENRY, Antoine/0000-0003-4952-5812; Macaisne, Nicolas/0000-0002-0109-9845; Cock, J. Mark/0000-0002-2650-0383	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [ANR-10-BLAN-1727, ANR-10-BTBR-04-01]; Interreg Program France (Channel)-England (project Marinexus); Universite-Pierre et Marie Curie; European Research CouncilEuropean Research Council (ERC)European Commission [638240]; China Scholarship CouncilChina Scholarship Council; Conseil Regional de Bretagne (SAD Program)Region Bretagne	This work was supported by the Centre National de la Recherche Scientifique; Agence Nationale de la Recherche (project Bi-cycle ANR-10-BLAN-1727 and project Idealg ANR-10-BTBR-04-01); Interreg Program France (Channel)-England (project Marinexus); Universite-Pierre et Marie Curie; and the European Research Council (grant agreement 638240). F.L. and M.-M.P. were supported by grants from the China Scholarship Council and the Conseil Regional de Bretagne (SAD Program), respectively.	Abeel T, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gkr995; Anders S, 2015, BIOINFORMATICS, V31, P166, DOI 10.1093/bioinformatics/btu638; Anders S, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-10-r106; Aoyama T, 2012, DEVELOPMENT, V139, P3120, DOI 10.1242/dev.076091; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Blanc G, 2010, PLANT CELL, V22, P2943, DOI 10.1105/tpc.110.076406; Bogen C, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-926; Bower FO, 1890, ANN BOT, V4, P347; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Caplen NJ, 2001, P NATL ACAD SCI USA, V98, P9742, DOI 10.1073/pnas.171251798; Celakovsky L, 1874, SITZUNGSBERICHTE KOE, V2, P21; Cock JM, 2015, ADV MAR GENOMICS, V2, P153, DOI 10.1007/978-94-017-9642-2_8; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; de Hoon MJL, 2004, BIOINFORMATICS, V20, P1453, DOI 10.1093/bioinformatics/bth078; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Dolan L, 2009, CURR OPIN PLANT BIOL, V12, P4, DOI 10.1016/j.pbi.2008.12.001; Farnham G, 2013, J PHYCOL, V49, P819, DOI 10.1111/jpy.12096; Fritch F. E., 1959, STRUCTURE REPROD ALG; Gobler CJ, 2011, P NATL ACAD SCI USA, V108, P4352, DOI 10.1073/pnas.1016106108; Gouy M, 2010, MOL BIOL EVOL, V27, P221, DOI 10.1093/molbev/msp259; Haig D, 2006, PHILOS T R SOC B, V361, P335, DOI 10.1098/rstb.2005.1794; Horn T, 2010, NUCLEIC ACIDS RES, V38, pW332, DOI 10.1093/nar/gkq317; Kawai H, 2003, PROTIST, V154, P211, DOI 10.1078/143446103322166518; Kawai H, 2015, J PHYCOL, V51, P918, DOI 10.1111/jpy.12332; Kim D, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-4-r36; Kubota A, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms4668; Li WZ, 2015, NUCLEIC ACIDS RES, V43, pW580, DOI 10.1093/nar/gkv279; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Love MI, 2014, GENOME BIOL, V15, DOI 10.1186/s13059-014-0550-8; Menand B, 2007, SCIENCE, V316, P1477, DOI 10.1126/science.1142618; Moreau H, 2012, GENOME BIOL, V13, DOI 10.1186/gb-2012-13-8-r74; Niklas KJ, 2010, NEW PHYTOL, V185, P27, DOI 10.1111/j.1469-8137.2009.03054.x; Nishiyama T, 2003, P NATL ACAD SCI USA, V100, P8007, DOI 10.1073/pnas.0932694100; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Pires ND, 2012, PHILOS T R SOC B, V367, P508, DOI 10.1098/rstb.2011.0252; Qiu YL, 2006, P NATL ACAD SCI USA, V103, P15511, DOI 10.1073/pnas.0603335103; R Development Core Team,, 2009, R LANG ENV STAT COMP; Read BA, 2013, NATURE, V499, P209, DOI 10.1038/nature12221; Saldanha AJ, 2004, BIOINFORMATICS, V20, P3246, DOI 10.1093/bioinformatics/bth349; Sano R, 2005, EVOL DEV, V7, P69, DOI 10.1111/j.1525-142X.2005.05008.x; Shaw AJ, 2011, AM J BOT, V98, P352, DOI 10.3732/ajb.1000316; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Szovenyi P, 2011, MOL BIOL EVOL, V28, P803, DOI 10.1093/molbev/msq254; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Qiu YL, 2008, J SYST EVOL, V46, P287, DOI [10.3724/SP.J.1002.2008.08035, 10.1111/j.1439-0469.2008.00465.x]	54	11	11	0	8	COMPANY BIOLOGISTS LTD	CAMBRIDGE	BIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND	0950-1991	1477-9129		DEVELOPMENT	Development	FEB 1	2017	144	3					409	418		10.1242/dev.141523			10	Developmental Biology	Developmental Biology	EK2OR	WOS:000393767100006	28049657	Green Published, Bronze			2021-04-07	
J	Toporkova, YY; Fatykhova, VS; Gogolev, YV; Khairutdinov, BI; Mukhtarova, LS; Grechkin, AN				Toporkova, Yana Y.; Fatykhova, Valeria S.; Gogolev, Yuri V.; Khairutdinov, Bulat I.; Mukhtarova, Lucia S.; Grechkin, Alexander N.			Epoxyalcohol synthase of Ectocarpus siliculosus. First CYP74-related enzyme of oxylipin biosynthesis in brown algae	BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS			English	Article						Oxylipins; Cytochrome P450; CYP74 clan; Epoxyalcohol synthase; Brown alga Ectocarpus siliculosus	LINOLEIC-ACID HYDROPEROXIDE; ALLENE OXIDE SYNTHASE; FATTY-ACIDS; EXPRESSION; CYP74; BIOGENESIS; MECHANISM; CATALYSIS; HYDROXY; REVEALS	Enzymes of CYP74 family play the central role in the biosynthesis of physiologically important oxylipins in land plants. Although a broad diversity of oxylipins is known in the algae, no CYP74s or related enzymes have been detected in brown algae yet. Cloning of the first CYP74-related gene CYP5164B1 of brown alga Ectocarpus siliculosus is reported in present work. The recombinant protein was incubated with several fatty acid hydroperoxides. Linoleic acid 9-hydroperoxide (9-HPOD) was the preferred substrate, while linoleate 13-hydroperoxide (13-HPOD) was less efficient. a.-Linolenic acid 9- and 13-hydroperoxides, as well as eicosapentaenoic acid 15-hydroperoxide were inefficient substrates. Both 9-HPOD and 13-HPOD were converted into epoxyalcohols. For instance, 9-HPOD was turned primarily into (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid. Both epoxide and hydroxyl oxygen atoms of the epoxyalcohol were incorporated mostly from [O-18(2)]9-HPOD. Thus, the enzyme exhibits the activity of epoxyalcohol synthase (EsEAS). The results show that the EsEAS isomerizes the hydroperoxides into epoxyalcohols via epoxyallylic radical, a common intermediate of different CYP74s and related enzymes. EsEAS can be considered as an archaic prototype of CYP74 family enzymes. (C) 2016 Elsevier B.V. All rights reserved.	[Toporkova, Yana Y.; Fatykhova, Valeria S.; Gogolev, Yuri V.; Khairutdinov, Bulat I.; Mukhtarova, Lucia S.; Grechkin, Alexander N.] Russian Acad Sci, Kazan Inst Biochem & Biophys, POB 30, Kazan 420111, Russia	Grechkin, AN (corresponding author), Russian Acad Sci, Kazan Inst Biochem & Biophys, POB 30, Kazan 420111, Russia.	grechkin@kibb.knc.ru	Khairutdinov, Bulat/L-5986-2013; Gogolev, Yuri V/E-7815-2014; Grechkin, Alexander N/K-2098-2018	Khairutdinov, Bulat/0000-0002-4753-0768; Grechkin, Alexander/0000-0002-1276-8792; Gogolev, Yuri/0000-0002-2391-2980	Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [16-34-00648-mol_a, 14-04-01532-a, 15-04-04108-a]; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10286];  [MK-6529.2015.4]	Experiments on the expression and purification of recombinant protein were supported by grant 16-34-00648-mol_a from the Russian Foundation for Basic Research (RFBR). Experiments on purification of reaction products by HPLC were supported by grant 14-04-01532-a from the RFBR. Experiments on GC-MS analyses of reaction products of the CYP5164B1 were supported by grant MK-6529.2015.4. Experiments on NMR analyses of reaction products of CYP5164B1 were supported by grant 15-04-04108-a from the RFBR. Studies of catalytic mechanism of the CYP5164B1 enzyme were carried out under financing from the Russian Science Foundation (Project No. 16-14-10286). The authors thank Dr. Fakhima Mukhitova for GC-MS records.	Andreou A, 2009, PROG LIPID RES, V48, P148, DOI 10.1016/j.plipres.2009.02.002; Barbosa M, 2016, MAR DRUGS, V14, DOI 10.3390/md14010023; BERNART MW, 1993, J NAT PROD, V56, P245, DOI 10.1021/np50092a010; BERNART MW, 1994, PHYTOCHEMISTRY, V36, P1233, DOI 10.1016/S0031-9422(00)89643-0; Brash AR, 2009, PHYTOCHEMISTRY, V70, P1522, DOI 10.1016/j.phytochem.2009.08.005; Chang MS, 1996, BIOCHEMISTRY-US, V35, P464, DOI 10.1021/bi952081v; Chechetkin IR, 2009, BIOCHEMISTRY-MOSCOW+, V74, P855, DOI 10.1134/S0006297909080069; Choi H, 2012, PHYTOCHEMISTRY, V73, P134, DOI 10.1016/j.phytochem.2011.09.014; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; DIX TA, 1985, J BIOL CHEM, V260, P5351; GARDNER HW, 1975, J AGR FOOD CHEM, V23, P129, DOI 10.1021/jf60198a012; GARDNER HW, 1984, J ORG CHEM, V49, P508, DOI 10.1021/jo00177a024; GARDNER HW, 1984, CHEM PHYS LIPIDS, V35, P87, DOI 10.1016/0009-3084(84)90015-X; Gerwick WH, 2002, LIPID BIOTECHNOLOGY, P249; Gerwick WH, 1999, ADV EXP MED BIOL, V447, P211; Gerwick WH, 1996, LIPIDS, V31, P1215, DOI 10.1007/BF02587906; Grechkin A, 1998, PROG LIPID RES, V37, P317, DOI 10.1016/S0163-7827(98)00014-9; Grechkin AN, 2006, BBA-MOL CELL BIOL L, V1761, P1419, DOI 10.1016/j.bbalip.2006.09.002; Grechkin AN, 2004, BBA-MOL CELL BIOL L, V1636, P47, DOI 10.1016/j.bbalip.2003.12.003; Grechkin AN, 2002, PROSTAG OTH LIPID M, V68-9, P457, DOI 10.1016/S0090-6980(02)00048-5; Hoffmann I, 2013, J LIPID RES, V54, P3471, DOI 10.1194/jlr.M044347; Hoffmann I, 2013, J BIOL CHEM, V288, P11459, DOI 10.1074/jbc.M113.458257; Hughes RK, 2008, PROTEINS, V72, P1199, DOI 10.1002/prot.22012; Hughes RK, 2009, CHEMBIOCHEM, V10, P1122, DOI 10.1002/cbic.200800633; JAMIESON GR, 1972, PHYTOCHEMISTRY, V11, P1423, DOI 10.1016/S0031-9422(00)90096-7; Jiang ZD, 1997, LIPIDS, V32, P231, DOI 10.1007/s11745-997-0029-9; Khotimchenko SV, 2002, BOT MAR, V45, P17, DOI 10.1515/BOT.2002.003; Koeduka T, 2015, PLANTA, V242, P1175, DOI 10.1007/s00425-015-2355-8; Kousaka K, 2003, J NAT PROD, V66, P1318, DOI 10.1021/np030049t; Lamari N, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0073281; Lee DS, 2008, NATURE, V455, P363, DOI 10.1038/nature07307; MERCIER J, 1974, CHEM PHYS LIPIDS, V12, P232, DOI 10.1016/0009-3084(74)90077-2; Nelson David R, 2006, Methods Mol Biol, V320, P1; PROTEAU PJ, 1994, J NAT PRODUCTS, V57, P1717, DOI 10.1021/np50114a016; PROTEAU PJ, 1993, LIPIDS, V28, P783, DOI 10.1007/BF02536231; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Schenkman John B, 2006, Methods Mol Biol, V320, P11; SONG WC, 1993, J BIOL CHEM, V268, P6293; Thomas CP, 2013, CHEM PHYS LIPIDS, V167, P21, DOI 10.1016/j.chemphyslip.2013.01.002; Wilson RA, 2001, MOL PLANT MICROBE IN, V14, P980, DOI 10.1094/MPMI.2001.14.8.980	42	7	8	1	16	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1388-1981	1879-2618		BBA-MOL CELL BIOL L	Biochim. Biophys. Acta Mol. Cell Biol. Lipids	FEB	2017	1862	2					167	175		10.1016/j.bbalip.2016.11.007			9	Biochemistry & Molecular Biology; Biophysics; Cell Biology	Biochemistry & Molecular Biology; Biophysics; Cell Biology	EJ0KU	WOS:000392898100004	27863255				2021-04-07	
J	Montecinos, AE; Couceiro, L; Peters, AF; Desrut, A; Valero, M; Guillemin, ML				Montecinos, Alejandro E.; Couceiro, Lucia; Peters, Akira F.; Desrut, Antoine; Valero, Myriam; Guillemin, Marie-Laure			SPECIES DELIMITATION AND PHYLOGEOGRAPHIC ANALYSES IN THE ECTOCARPUS SUBGROUP SILICULOSI (ECTOCARPALES, PHAEOPHYCEAE)	JOURNAL OF PHYCOLOGY			English	Article						barcode; brown alga; COI-5P; cryptic species; Ectocarpus; hybridization; introgression; ITS1; species delimitation	GENETIC DIVERSITY; PHYLOGENETIC ANALYSIS; GENUS ECTOCARPUS; BROWN-ALGAE; DNA; PACIFIC; HYBRIDIZATION; PATTERNS; SOUTH; BIOGEOGRAPHY	The genus Ectocarpus (Ectocarpales, Phaeophyceae) contains filamentous algae widely distributed in marine and estuarine habitats of temperate regions in both hemispheres. While E. siliculosus has become a model organism for genomics and genetics of the brown macroalgae, accurate species delineation, distribution patterns and diversity for the genus Ectocarpus remain problematic. In this study, we used three independent species delimitation approaches to generate a robust species hypothesis for 729 Ectocarpus specimens collected mainly along the European and Chilean coasts. These approaches comprised phylogenetic reconstructions and two bioinformatics tools developed to objectively define species boundaries (General Mixed Yule Coalescence Method and Automatic Barcode Gap Discovery). Our analyses were based on DNA sequences of two loci: the mitochondrial cytochrome oxidase subunit 1 and the nuclear internal transcribed spacer 1 of the ribosomal DNA. Our analyses showed the presence of at least 15 cryptic species and suggest the existence of incomplete lineage sorting or introgression between five of them. These results suggested the possible existence of different levels of reproductive barriers within this species complex. We also detected differences among species in their phylogeographic patterns, range and depth distributions, which may suggest different biogeographic histories (e.g., endemic species or recent introductions).	[Montecinos, Alejandro E.; Couceiro, Lucia; Desrut, Antoine; Valero, Myriam; Guillemin, Marie-Laure] UPMC Univ Paris VI, CNRS, Sorbonne Univ,PUC,UACH,UMI 3614,CS 90074, Evolutionary Biol & Ecol Algae,Stn Biol Roscoff, Pl G Teissier, F-29680 Roscoff, France; [Montecinos, Alejandro E.; Guillemin, Marie-Laure] Univ Austral Chile, Fac Ciencias, Inst Ciencias Ambientales & Evolut, Casilla 567, Valdivia, Chile; [Peters, Akira F.] Bezhin Rosko, 40 Rue Pecheurs, F-29250 Santec, France; [Couceiro, Lucia] Univ A Coruna, Dept Anim Biol Plant Biol & Ecol, La Coruna, Spain	Guillemin, ML (corresponding author), UPMC Univ Paris VI, CNRS, Sorbonne Univ,PUC,UACH,UMI 3614,CS 90074, Evolutionary Biol & Ecol Algae,Stn Biol Roscoff, Pl G Teissier, F-29680 Roscoff, France.; Guillemin, ML (corresponding author), Univ Austral Chile, Fac Ciencias, Inst Ciencias Ambientales & Evolut, Casilla 567, Valdivia, Chile.	marielaure.guillemin@gmail.com	Couceiro, Lucia/M-1851-2014; Valero, Myriam/M-6052-2019	Couceiro, Lucia/0000-0003-4300-5744; Valero, Myriam/0000-0002-9000-1423; Desrut, Antoine/0000-0003-3876-915X; Couceiro, Lucia/0000-0001-9466-8164	CONICYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT); IDEALG (France) [ANR-10-BTBR-04]; INRA-MNHN-INEE-CNRS; Bi-Cycle (France) [ANR10- BLAN-1727]; EU FP7European Commission [227799]; EU Interreg program France (Channel)-England; TOTAL Foundation; Ray- Lankester Fellowship (UK)	We are particularly grateful to C. Destombe, S. Faugeron, A. Mann, D. G. Muller, P. Murua, D. Patino, D. Schroeder, J. West and R. Westermeier, who helped by collecting samples. The authors thank Biogenouest Core Facility for technical assistance and the members of Grupo BioCost of Coruna University for advice and logistic support during sampling in Galicia and North Portugal. We also thank to S. Mauger for assistance in the molecular laboratory and L. Dartevelle and J. Coudret for help in maintaining strains in the Station Biologique de Roscoff. We particularly appreciated insightful and constructive comments from C. Destombe and J. M. Cock. Principal funding came from a doctoral grant to A. E. Montecinos (Becas-Chile, CONICYT, advanced human resources program), additional support from the projects IDEALG (France: ANR-10-BTBR-04), "Bibliotheque du vivant" (France: INRA-MNHN-INEE-CNRS) and Bi-Cycle (France: ANR10- BLAN-1727), the EU FP7 "capacities" specific program ASSEMBLE (grant no. 227799), the EU Interreg program France (Channel)-England (project Marinexus), the TOTAL Foundation (Project "Brown algal biodiversity and ecology in the Eastern Mediterranean Sea"), and a Ray- Lankester Fellowship (UK: Marine Biological Association, Plymouth) to A. F. Peters. The work was carried out within the context of the international research network "Diversity, Evolution and Biotechnology of Marine Algae" (GDRI No. 0803). The authors have declared that no competing interests exist.	Allendorf FW, 2001, TRENDS ECOL EVOL, V16, P613, DOI 10.1016/S0169-5347(01)02290-X; Alo D, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0071577; Bandelt HJ, 1999, MOL BIOL EVOL, V16, P37, DOI 10.1093/oxfordjournals.molbev.a026036; Camus PA, 2001, REV CHIL HIST NAT, V74, P587, DOI 10.4067/S0716-078X2001000300008; Canovas FG, 2011, BMC EVOL BIOL, V11, DOI 10.1186/1471-2148-11-371; Cardenas L, 2009, J BIOGEOGR, V36, P969, DOI 10.1111/j.1365-2699.2008.02056.x; Cardinal A, 1964, ETUDE ECTOCARPACEES; Carstens BC, 2013, MOL ECOL, V22, P4369, DOI 10.1111/mec.12413; Carstens BC, 2013, BIOL J LINN SOC, V109, P737, DOI 10.1111/bij.12093; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cornils A, 2014, FRONT ZOOL, V11, DOI 10.1186/1742-9994-11-19; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; Coyer JA, 2011, MOL PHYLOGENET EVOL, V58, P283, DOI 10.1016/j.ympev.2010.11.015; Coyne J.A., 2004, SPECIATION; Darriba D, 2012, NAT METHODS, V9, P772, DOI 10.1038/nmeth.2109; DiBattista JD, 2012, J HERED, V103, P617, DOI 10.1093/jhered/ess056; Dillwyn L. W, 1809, BRIT CONFERVAE COLOR, P1; Drummond AJ, 2012, MOL BIOL EVOL, V29, P1969, DOI 10.1093/molbev/mss075; Dupuis JR, 2012, MOL ECOL, V21, P4422, DOI 10.1111/j.1365-294X.2012.05642.x; EXCOFFIER L, 1992, GENETICS, V131, P479; Excoffier L, 2010, MOL ECOL RESOUR, V10, P564, DOI 10.1111/j.1755-0998.2010.02847.x; Geoffroy A, 2015, J PHYCOL, V51, P480, DOI 10.1111/jpy.12291; Goldstein PZ, 2011, BIOESSAYS, V33, P135, DOI 10.1002/bies.201000036; Guillemin M.-L., 2016, SEAWEED PHYLOGEOGRAP, P251, DOI DOI 10.1007/978-94-017-7534-2_10; Guillemin ML, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0114039; Hamel G., 1931, PHEOPHYCEES FRANCE; Haye PA, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0088613; Hebert PDN, 2003, P ROY SOC B-BIOL SCI, V270, P313, DOI 10.1098/rspb.2002.2218; Hedtke SM, 2006, SYST BIOL, V55, P522, DOI 10.1080/10635150600697358; Hillis DM, 2003, SYST BIOL, V52, P124, DOI 10.1080/10635150390132911; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; Kekkonen M, 2014, MOL ECOL RESOUR, V14, P706, DOI 10.1111/1755-0998.12233; Knowles LL, 2004, J EVOLUTION BIOL, V17, P1, DOI 10.1046/j.1420-9101.2003.00644.x; Lane CE, 2007, MOL PHYLOGENET EVOL, V44, P634, DOI 10.1016/j.ympev.2007.03.016; LECOINTRE G, 1993, MOL PHYLOGENET EVOL, V2, P205, DOI 10.1006/mpev.1993.1021; Librado P, 2009, BIOINFORMATICS, V25, P1451, DOI 10.1093/bioinformatics/btp187; Lyngbye H.C., 1819, TENTAMEN HYDROPHYTOL; Maggs CA, 2008, ECOLOGY, V89, pS108, DOI 10.1890/08-0257.1; Mallet J, 2005, TRENDS ECOL EVOL, V20, P229, DOI 10.1016/j.tree.2005.02.010; Mayden R.L., 1997, Systematics Association Special Volume Series, V54, P381; Mayden RL, 1999, J NEMATOL, V31, P95; Modica MV, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0102160; Monaghan MT, 2009, SYST BIOL, V58, P298, DOI 10.1093/sysbio/syp027; Muller D. G., 1991, JPN J PHYCOL, V39, P151; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1976, J PHYCOL, V12, P252, DOI 10.1111/j.0022-3646.1976.00252.x; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; MULLER DG, 1977, BRIT PHYCOL J, V12, P131; NEI M, 1979, P NATL ACAD SCI USA, V76, P5269, DOI 10.1073/pnas.76.10.5269; NEIVA J, 2016, SEAWEED PHYLOGEOGRAP, P279, DOI DOI 10.1007/978-94-017-7534-2; Neiva J, 2012, BMC EVOL BIOL, V12, DOI 10.1186/1471-2148-12-78; Pante E, 2015, MOL ECOL, V24, P525, DOI 10.1111/mec.13048; Pardo C, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0104073; Payo DA, 2013, P ROY SOC B-BIOL SCI, V280, DOI 10.1098/rspb.2012.2660; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; PETERS AF, 1993, MAR BIOL, V115, P143, DOI 10.1007/BF00349396; Peters AF, 2015, CRYPTOGAMIE ALGOL, V36, P3, DOI 10.7872/crya.v36.iss1.2015.3; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Peters JL, 2007, EVOLUTION, V61, P1992, DOI 10.1111/j.1558-5646.2007.00149.x; Pons J, 2006, SYST BIOL, V55, P595, DOI 10.1080/10635150600852011; Prevot V, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0060736; Puillandre N, 2012, MOL ECOL, V21, P2671, DOI 10.1111/j.1365-294X.2012.05559.x; Puillandre N, 2012, MOL ECOL, V21, P1864, DOI 10.1111/j.1365-294X.2011.05239.x; Raimondi PT, 2004, ECOLOGY, V85, P3267, DOI 10.1890/03-0559; Rambaut A, 2010, TREEANNOTATOR VERSIO; REED DC, 1990, ECOLOGY, V71, P776, DOI 10.2307/1940329; Robuchon M, 2014, MOL ECOL, V23, P2669, DOI 10.1111/mec.12774; RUSSELL G, 1967, J MAR BIOL ASSOC UK, V47, P233, DOI 10.1017/S0025315400033695; RUSSELL G, 1983, MAR ECOL PROG SER, V13, P303, DOI 10.3354/meps013303; RUSSELL G, 1983, MAR ECOL PROG SER, V11, P181, DOI 10.3354/meps011181; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; RUSSELL G, 1967, HELGOLAND WISS MEER, V15, P155, DOI 10.1007/BF01618619; Sites JW, 2004, ANNU REV ECOL EVOL S, V35, P199, DOI 10.1146/annurev.ecolsys.35.112202.130128; Sites JW, 2003, TRENDS ECOL EVOL, V18, P462, DOI 10.1016/S0169-5347(03)00184-8; Smith BT, 2011, BMC EVOL BIOL, V11, DOI 10.1186/1471-2148-11-136; Spalding MD, 2007, BIOSCIENCE, V57, P573, DOI 10.1641/B570707; Stache B, 1990, EVOLUTIONARY BIOGEOG, P173; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Stamatakis A, 2008, SYST BIOL, V57, P758, DOI 10.1080/10635150802429642; Stamatakis A, 2014, BIOINFORMATICS, V30, P1312, DOI 10.1093/bioinformatics/btu033; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tronholm A, 2010, J PHYCOL, V46, P1301, DOI 10.1111/j.1529-8817.2010.00908.x; Vieira C, 2014, J PHYCOL, V50, P1101, DOI 10.1111/jpy.12243; Wiens JJ, 2002, SYST BIOL, V51, P69, DOI 10.1080/106351502753475880; Yamashita T, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0068282; Yu Y, 2011, SYST BIOL, V60, P138, DOI 10.1093/sysbio/syq084	88	30	30	1	22	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	FEB	2017	53	1					17	31		10.1111/jpy.12452			15	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	EP3CJ	WOS:000397259800004	27454456				2021-04-07	
J	Prigent, S; Frioux, C; Dittami, SM; Thiele, S; Larhlimi, A; Collet, G; Gutknecht, F; Got, J; Eveillard, D; Bourdon, J; Plewniak, F; Tonon, T; Siegel, A				Prigent, Sylvain; Frioux, Clemence; Dittami, Simon M.; Thiele, Sven; Larhlimi, Abdelhalim; Collet, Guillaume; Gutknecht, Fabien; Got, Jeanne; Eveillard, Damien; Bourdon, Jeremie; Plewniak, Frederic; Tonon, Thierry; Siegel, Anne			Meneco, a Topology-Based Gap-Filling Tool Applicable to Degraded Genome-Wide Metabolic Networks	PLOS COMPUTATIONAL BIOLOGY			English	Article							MINE DRAINAGE BIOINDICATOR; LIPID-COMPOSITION; SCALE RECONSTRUCTION; EUGLENA-GRACILIS; RESOURCE; MODEL; OPTIMIZATION; CURATION; PROTEIN	Increasing amounts of sequence data are becoming available for a wide range of non model organisms. Investigating and modelling the metabolic behaviour of those organisms is highly relevant to understand their biology and ecology. As sequences are often incomplete and poorly annotated, draft networks of their metabolism largely suffer from incompleteness. Appropriate gap-filling methods to identify and add missing reactions are therefore required to address this issue. However, current tools rely on phenotypic or taxonomic information, or are very sensitive to the stoichiometric balance of metabolic reactions, especially concerning the co-factors. This type of information is often not available or at least prone to errors for newly-explored organisms. Here we introduce Meneco, a tool dedicated to the topological gap-filling of genome-scale draft metabolic networks. Meneco reformulates gap-filling as a qualitative combinatorial optimization problem, omitting constraints raised by the stoichiometry of a metabolic network considered in other methods, and solves this problem using Answer Set Programming. Run on several artificial test sets gathering 10,800 degraded Escherichia colt networks Meneco was able to efficiently identify essential reactions missing in networks at high degradation rates, outperforming the stoichiometry-based tools in scalability. To demonstrate the utility of Meneco we applied it to two case studies. Its application to recent metabolic networks reconstructed for the brown algal model Ectocarpus siliculosus and an associated bacterium Candidatus Phaeomarinobacter ectocarpi revealed several candidate metabolic pathways for algal-bacterial interactions. Then Meneco was used to reconstruct, from transcriptomic and metabolomic data, the first metabolic network for the microalga Euglena mutabilis. These two case studies show that Meneco is a versatile tool to complete draft genome-scale metabolic networks produced from heterogeneous data, and to suggest relevant reactions that explain the metabolic capacity of a biological system.	[Prigent, Sylvain; Frioux, Clemence; Collet, Guillaume; Got, Jeanne; Siegel, Anne] Univ Rennes 1, Inst Res IT & Random Syst IRISA, Rennes, France; [Prigent, Sylvain] Chalmers Univ Technol, Dept Biol & Biol Engn, Gothenburg, Sweden; [Prigent, Sylvain; Frioux, Clemence; Collet, Guillaume; Got, Jeanne; Siegel, Anne] CNRS, Irisa, Rennes, France; [Prigent, Sylvain; Frioux, Clemence; Thiele, Sven; Collet, Guillaume; Got, Jeanne; Siegel, Anne] Inria, Dyliss, Rennes, France; [Dittami, Simon M.; Tonon, Thierry] UPMC Univ Paris 06, Stn Biolog Roscoff, CNRS,UMR 8227, Integrat Biol Marine Models,Sorbonne Univ, Roscoff, France; [Larhlimi, Abdelhalim; Eveillard, Damien; Bourdon, Jeremie] Univ Nantes, Comp Sci Lab Nantes Atlantique, LINA UMR6241, Nantes, France; [Gutknecht, Fabien; Plewniak, Frederic] Univ Strasbourg, Mol Genet Genom & Microbiol GMGM, Strasbourg, France; [Plewniak, Frederic] CNRS, GMGM, Strasbourg, France; [Thiele, Sven] Max Planck Inst Dynam Complex Tech Syst, Magdeburg, Germany; [Tonon, Thierry] Univ York, Ctr Novel Agr Prod, Dept Biol, York, N Yorkshire, England	Prigent, S (corresponding author), Univ Rennes 1, Inst Res IT & Random Syst IRISA, Rennes, France.; Prigent, S (corresponding author), Chalmers Univ Technol, Dept Biol & Biol Engn, Gothenburg, Sweden.; Prigent, S (corresponding author), CNRS, Irisa, Rennes, France.; Prigent, S (corresponding author), Inria, Dyliss, Rennes, France.	prigent@chalmers.se; anne.siegel@irisa.fr	Tonon, Thierry/A-3214-2009; Frioux, Clemence/A-1517-2019; eveillard, damien/AAQ-2363-2020	Tonon, Thierry/0000-0002-1454-6018; Frioux, Clemence/0000-0003-2114-0697; eveillard, damien/0000-0002-8162-7360; Bourdon, Jeremie/0000-0001-6674-8626; Plewniak, Frederic/0000-0001-6534-8786; Larhlimi, Abdelhalim/0000-0002-1709-2648; Prigent, Sylvain/0000-0001-5146-0347; Thiele, Sven/0000-0002-5812-6963; Siegel, Anne/0000-0001-6542-1568	French Government via the National Research Agency investment expenditure program IDEALG [ANR-10-BTBR-04]; French national program EC2COMicrobiEn, CoMMERCE	This work benefited from the support of the French Government via the National Research Agency investment expenditure program IDEALG ANR-10-BTBR-04. The reconstruction of the Euglena mutabilis metabolic network was supported by the French national program EC2COMicrobiEn, CoMMERCE. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Agren R, 2013, PLOS COMPUT BIOL, V9, DOI 10.1371/journal.pcbi.1002980; Benedict MN, 2014, PLOS COMPUT BIOL, V10, DOI 10.1371/journal.pcbi.1003882; BUETOW DE, 1962, J GEN MICROBIOL, V28, P579, DOI 10.1099/00221287-28-4-579; Caspi R, 2014, NUCLEIC ACIDS RES, V42, pD459, DOI 10.1093/nar/gkt1103; Christian N, 2009, MOL BIOSYST, V5, P1889, DOI [10.1039/b915913b, 10.1039/B915913b]; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Collet G, 2013, LECT NOTES COMPUT SC, V8148, P245, DOI 10.1007/978-3-642-40564-8_25; CONSTANT.G, 1970, PLANT PHYSIOL, V45, P76, DOI 10.1104/pp.45.1.76; CONSTANTOPOULOS G, 1967, J BIOL CHEM, V242, P3538; Cottret L, 2008, LECT N BIOINFORMAT, V5251, P233, DOI 10.1007/978-3-540-87361-7_20; Czyzyk J, 1998, IEEE COMPUT SCI ENG, V5, P68, DOI 10.1109/99.714603; de Figueiredo LF, 2009, BIOINFORMATICS, V25, P152, DOI 10.1093/bioinformatics/btn621; Dittami SM, 2014, FRONT GENET, V5, DOI 10.3389/fgene.2014.00241; Dittami SM, 2014, MOL ECOL, V23, P1656, DOI 10.1111/mec.12670; Ebrahim A, 2013, BMC SYST BIOL, V7, DOI 10.1186/1752-0509-7-74; Feist AM, 2007, MOL SYST BIOL, V3, DOI 10.1038/msb4100155; Forster J, 2003, GENOME RES, V13, P244, DOI 10.1101/gr.234503; Gebser M, 2008, LECT NOTES COMPUT SC, V5366, P190, DOI 10.1007/978-3-540-89982-2_23; Gebser M., 2012, SYNTHESIS LECT ARTIF, V6, P1, DOI DOI 10.2200/S00457ED1V01Y201211AIM019; Gebser M, 2012, ARTIF INTELL, V187, P52, DOI 10.1016/j.artint.2012.04.001; Halter D, 2015, ENVIRON MICROBIOL, V17, P1941, DOI 10.1111/1462-2920.12474; Halter D, 2012, ISME J, V6, P1391, DOI 10.1038/ismej.2011.198; Halter D, 2012, APPL MICROBIOL BIOT, V93, P1735, DOI 10.1007/s00253-011-3493-y; Handorf T, 2005, J MOL EVOL, V61, P498, DOI 10.1007/s00239-005-0027-1; Henry CS, 2010, NAT BIOTECHNOL, V28, P977, DOI 10.1038/nbt.1672; Holmstrom K, 1999, ADV MODEL OPTIM, V1, P47; Karp Peter D, 2002, Bioinformatics, V18 Suppl 1, pS225; KOTT Y, 1964, APPL MICROBIOL, V12, P292, DOI 10.1128/AEM.12.4.292-294.1964; Kumar VS, 2007, BMC BIOINFORMATICS, V8, DOI 10.1186/1471-2105-8-212; Loira N, 2015, J BIOINF COMPUT BIOL, V13, DOI 10.1142/S0219720015500067; Lunau M, 2005, ENVIRON MICROBIOL, V7, P961, DOI 10.1111/j.1462-2920.2005.00767.x; Maranas CD, 2009, PLOS COMPUT BIOL, V5, DOI [DOI 10.1371/JOURNAL.PCBI.1000308, 10.1371/journal.pcbi.1000308]; Marashi SA, 2014, J THEOR BIOL, V355, P185, DOI 10.1016/j.jtbi.2014.04.011; Marchetti-Spaccamela A, 2010, PLOS COMPUTATIONAL B, V6, P1; Mintz-Oron S, 2012, P NATL ACAD SCI USA, V109, P339, DOI 10.1073/pnas.1100358109; Monk J, 2014, NAT BIOTECHNOL, V32, P447, DOI 10.1038/nbt.2870; Orth JD, 2011, MOL SYST BIOL, V7, DOI 10.1038/msb.2011.65; Orth JD, 2010, NAT BIOTECHNOL, V28, P245, DOI 10.1038/nbt.1614; Pharkya P, 2004, GENOME RES, V14, P2367, DOI 10.1101/gr.2872004; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; Reed JL, 2006, P NATL ACAD SCI USA, V103, P17480, DOI 10.1073/pnas.0603364103; Reed JL, 2003, GENOME BIOL, V4, DOI 10.1186/gb-2003-4-9-r54; REGNAULT A, 1995, PHYTOCHEMISTRY, V40, P725, DOI 10.1016/0031-9422(95)00268-C; Romero P R, 2001, Pac Symp Biocomput, P471; Schaub T, 2009, LECT NOTES COMPUT SC, V5649, P312, DOI 10.1007/978-3-642-02846-5_27; Steffensen JL, 2016, PLOS COMPUT BIOL, V12, DOI 10.1371/journal.pcbi.1004732; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; TERRY OW, 1970, PLANTA, V93, P106, DOI 10.1007/BF00387119; Thiele I, 2014, BIOINFORMATICS, V30, P2529, DOI 10.1093/bioinformatics/btu321; Thiele I, 2010, NAT PROTOC, V5, P93, DOI 10.1038/nprot.2009.203; Vallenet D, 2013, NUCLEIC ACIDS RES, V41, pE636, DOI 10.1093/nar/gks1194; Vitkin E, 2012, GENOME BIOL, V13, DOI 10.1186/gb-2012-13-11-r111; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986	53	28	29	0	7	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA		1553-7358		PLOS COMPUT BIOL	PLoS Comput. Biol.	JAN	2017	13	1							e1005276	10.1371/journal.pcbi.1005276			32	Biochemical Research Methods; Mathematical & Computational Biology	Biochemistry & Molecular Biology; Mathematical & Computational Biology	EK7ZT	WOS:000394144400017	28129330	DOAJ Gold, Green Published, Green Accepted			2021-04-07	
J	Nishitsuji, K; Arimoto, A; Iwai, K; Sudo, Y; Hisata, K; Fujie, M; Arakaki, N; Kushiro, T; Konishi, T; Shinzato, C; Satoh, N; Shoguchi, E				Nishitsuji, Koki; Arimoto, Asuka; Iwai, Kenji; Sudo, Yusuke; Hisata, Kanako; Fujie, Manabu; Arakaki, Nana; Kushiro, Tetsuo; Konishi, Teruko; Shinzato, Chuya; Satoh, Noriyuki; Shoguchi, Eiichi			A draft genome of the brown alga, Cladosiphon okamuranus, S-strain: a platformfor future studies of 'mozuku' biology	DNA RESEARCH			English	Article						brown alga; Cladosiphon okamuranus; genome decoding; genes for enzymes of polysaccharide synthesis	SEQUENCE ALIGNMENT; EVOLUTION; CELL; POLYSACCHARIDES; IDENTIFICATION; PHAEOPHYCEAE; REVEALS; PROGRAM; PATHWAY; SIZE	The brown alga, Cladosiphon okamuranus (Okinawa mozuku), is economically one of the most important edible seaweeds, and is cultivated for market primarily in Okinawa, Japan. C. okamuranus constitutes a significant source of fucoidan, which has various physiological and biological activities. To facilitate studies of seaweed biology, we decoded the draft genome of C. okamuranus S-strain. The genome size of C. okamuranus was estimated as similar to 140 Mbp, smaller than genomes of two other brown algae, Ectocarpus siliculosus and Saccharina japonica. Sequencing with similar to 100x coverage yielded an assembly of 541 scaffolds with N50 = 416 kbp. Together with transcriptomic data, we estimated that the C. okamuranus genome contains 13,640 protein-coding genes, approximately 94% of which have been confirmed with corresponding mRNAs. Comparisons with the E. siliculosus genome identified a set of C. okamuranus genes that encode enzymes involved in biosynthetic pathways for sulfated fucans and alginate biosynthesis. In addition, we identified C. okamuranus genes for enzymes involved in phlorotannin biosynthesis. The present decoding of the Cladosiphon okamuranus genome provides a platform for future studies of mozuku biology.	[Nishitsuji, Koki; Arimoto, Asuka; Hisata, Kanako; Shinzato, Chuya; Satoh, Noriyuki; Shoguchi, Eiichi] Grad Univ, Okinawa Inst Sci & Technol, Marine Genom Unit, Onna, Okinawa 9040495, Japan; [Iwai, Kenji; Sudo, Yusuke] Okinawa Prefectural Fisheries Res & Extens Ctr, Itoman, Okinawa 9010354, Japan; [Fujie, Manabu; Arakaki, Nana] Grad Univ, Okinawa Inst Sci & Technol, DNA Sequencing Sect, Onna, Okinawa 9040495, Japan; [Kushiro, Tetsuo] Meiji Univ, Sch Agr, Kawasaki, Kanagawa 2148571, Japan; [Konishi, Teruko] Univ Ryukyus, Fac Agr, Dept Biosci & Biotechnol, Nishihara, Okinawa 9030213, Japan	Nishitsuji, K; Shoguchi, E (corresponding author), Grad Univ, Okinawa Inst Sci & Technol, Marine Genom Unit, Onna, Okinawa 9040495, Japan.	koki.nishitsuji@oist.jp; eiichi@oist.jp	Satoh, Nori/C-4123-2009	Satoh, Nori/0000-0002-4480-3572; Shinzato, Chuya/0000-0001-7843-3381	OIST	This study was supported by OIST internal funding for the Marine Genomics Unit.	Adl SM, 2005, J EUKARYOT MICROBIOL, V52, P399, DOI 10.1111/j.1550-7408.2005.00053.x; Amsler CD, 2006, ADV BOT RES, V43, P1, DOI 10.1016/S0065-2296(05)43001-3; BABA M, 1988, ANTIMICROB AGENTS CH, V32, P1742, DOI 10.1128/AAC.32.11.1742; Benson G, 1999, NUCLEIC ACIDS RES, V27, P573, DOI 10.1093/nar/27.2.573; Bentley DR, 2006, CURR OPIN GENET DEV, V16, P545, DOI 10.1016/j.gde.2006.10.009; BOETTCHER AA, 1993, ECOLOGY, V74, P891, DOI 10.2307/1940814; Boetzer M, 2011, BIOINFORMATICS, V27, P578, DOI 10.1093/bioinformatics/btq683; Bolger AM, 2014, BIOINFORMATICS, V30, P2114, DOI 10.1093/bioinformatics/btu170; Cavalier-Smith T, 1999, J EUKARYOT MICROBIOL, V46, P347, DOI 10.1111/j.1550-7408.1999.tb04614.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Connan S, 2011, AQUAT TOXICOL, V104, P1, DOI 10.1016/j.aquatox.2011.03.016; DAKORA FD, 1995, AUST J PLANT PHYSIOL, V22, P87, DOI 10.1071/PP9950087; Eddy SR, 1998, BIOINFORMATICS, V14, P755, DOI 10.1093/bioinformatics/14.9.755; Finn RD, 2006, NUCLEIC ACIDS RES, V34, pD247, DOI 10.1093/nar/gkj149; Gotz S, 2011, BIOINFORMATICS, V27, P919, DOI 10.1093/bioinformatics/btr059; Gouy M, 2010, MOL BIOL EVOL, V27, P221, DOI 10.1093/molbev/msp259; Haas BJ, 2003, NUCLEIC ACIDS RES, V31, P5654, DOI 10.1093/nar/gkg770; Hirakawa H, 2014, DNA RES, V21, P169, DOI 10.1093/dnares/dst049; Huang SF, 2012, GENOME RES, V22, P1581, DOI 10.1101/gr.133652.111; Jurka J, 2005, CYTOGENET GENOME RES, V110, P462, DOI 10.1159/000084979; Jurka J, 1996, COMPUT CHEM, V20, P119, DOI 10.1016/S0097-8485(96)80013-1; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Keeling PJ, 2009, J EUKARYOT MICROBIOL, V56, P1, DOI 10.1111/j.1550-7408.2008.00371.x; Kraskov A, 2005, EUROPHYS LETT, V70, P278, DOI 10.1209/epl/i2004-10483-y; Le Lann K, 2012, MAR ENVIRON RES, V80, P1, DOI 10.1016/j.marenvres.2012.05.011; Li RQ, 2010, NATURE, V463, P311, DOI 10.1038/nature08696; LIN TY, 1966, J BIOL CHEM, V241, P5284; Marcais G, 2011, BIOINFORMATICS, V27, P764, DOI 10.1093/bioinformatics/btr011; Meslet-Cladiere L, 2013, PLANT CELL, V25, P3089, DOI 10.1105/tpc.113.111336; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Parra G, 2007, BIOINFORMATICS, V23, P1061, DOI 10.1093/bioinformatics/btm071; Pavia H, 2000, ECOLOGY, V81, P3212; Peng Y, 2012, BIOINFORMATICS, V28, P1420, DOI 10.1093/bioinformatics/bts174; Price AL, 2005, BIOINFORMATICS, V21, pI351, DOI 10.1093/bioinformatics/bti1018; Schulz MH, 2012, BIOINFORMATICS, V28, P1086, DOI 10.1093/bioinformatics/bts094; SIEBURTH JM, 1965, NATURE, V205, P830, DOI 10.1038/205830b0; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; Skinner ME, 2009, GENOME RES, V19, P1630, DOI 10.1101/gr.094607.109; Stanke M, 2008, BIOINFORMATICS, V24, P637, DOI 10.1093/bioinformatics/btn013; Szekely GJ, 2005, J CLASSIF, V22, P151, DOI 10.1007/s00357-005-0012-9; Takeuchi T, 2012, DNA RES, V19, P117, DOI 10.1093/dnares/dss005; Tako M, 1999, BIOSCI BIOTECH BIOCH, V63, P1813, DOI 10.1271/bbb.63.1813; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Thomas NV, 2011, ENVIRON TOXICOL PHAR, V32, P325, DOI 10.1016/j.etap.2011.09.004; Trapnell C, 2010, NAT BIOTECHNOL, V28, P511, DOI 10.1038/nbt.1621; Trapnell C, 2009, BIOINFORMATICS, V25, P1105, DOI 10.1093/bioinformatics/btp120; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; Wu YW, 2014, MICROBIOME, V2, DOI 10.1186/2049-2618-2-26; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; YOSHIDA T, 2015, JAPANESE J PHYCOLOGY, V63, P129; Zerbino DR, 2008, GENOME RES, V18, P821, DOI 10.1101/gr.074492.107	51	38	39	0	16	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	1340-2838	1756-1663		DNA RES	DNA Res.	DEC	2016	23	6					561	570		10.1093/dnares/dsw039			10	Genetics & Heredity	Genetics & Heredity	EI7ZJ	WOS:000392723300006	27501718	DOAJ Gold, Green Published			2021-04-07	
J	Kerrison, PD; Le, HN; Twigg, GC; Smallman, DR; MacPhee, R; Houston, FAB; Hughes, AD				Kerrison, Philip D.; Le, Hau Nhu; Twigg, Gail C.; Smallman, Duncan R.; MacPhee, Rory; Houston, Fiona A. B.; Hughes, Adam D.			Decontamination treatments to eliminate problem biota from macroalgal tank cultures of Osmundea pinnatifida, Palmaria palmata and Ulva lactuca	JOURNAL OF APPLIED PHYCOLOGY			English	Article						Decontamination; Epibiont; Epiphyte; Hypochlorite; Iodide; Macroalgae	OPEN-SEA CULTIVATION; CHLOROPHYLL FLUORESCENCE; LAMINARIA-SACCHARINA; TISSUE-CULTURE; ALGA; BIOFILTER; GROWTH; LIGHT; STERILIZATION; INTESTINALIS	The effect of a range of chemical disinfectants at different concentration and exposure times was investigated on five macroalgal species and the marine gastropod Littorina spp. Palmaria palmata, Osmundea pinnatifida and Ulva lactuca are commercially valuable and are often cultivated in tanks for food or feed. Ectocarpus siliculosus and Ulva intestinalis are common epiphytes of P. palmata and O. pinnatifida cultures, whilst Littorina spp. are common herbivorous epibionts within U. lactuca culture tanks. These contaminants reduce the productivity and quality of the culture as a food. Differential tolerance to the treatments was seen between the algal species using pulse-amplitude modulation (PAM) chlorophyll a fluorescence, a few hours and a week following treatment. We identified treatments that selectively damaged the epiphyte but not the basiphyte species. Ectocarpus siliculosus had a significantly lower tolerance to 1 % sodium hypochlorite than P. palmata, and to 25 % methanol than O. pinnatifida, with a 1-5 min exposure appearing most suitable. Ulva intestinalis had a significantly lower tolerance than P. palmata and O. pinnatifida to many disinfectants: 0.1-1 % sodium hypochlorite for 10 min, 0.5 % potassium iodide for up to 10 min, and 0.25 % Kick-start (a commercial aquaculture disinfectant solution) for 1-5 min. No treatment was able to kill the gastropod snails without also damaging U. lactuca, although agitation in freshwater for an hr may cause them to detach from the basiphyte, with little to no photophysiological impact seen to U. lactuca. This experiment forms the basis for more extended commercial trials.	[Kerrison, Philip D.; Twigg, Gail C.] Scottish Marine Inst, SAMS, Dunbeg PA37 1QA, Argyll, Scotland; [Le, Hau Nhu] Vietnam Acad Sci & Technol VAST, Nha Trang Inst Technol & Applicat NITRA, 2 Hung Vuong St, Nha Trang City, Vietnam; [Smallman, Duncan R.] Slate Islands Seaweed Ltd, 1B Easdale Isl, Oban PA34 4TB, Argyll, Scotland; [MacPhee, Rory; Houston, Fiona A. B.] Celt Sea Spice Co, 6 Long Craig Rigg, Edinburgh EH5 1QT, Midlothian, Scotland	Kerrison, PD (corresponding author), Scottish Marine Inst, SAMS, Dunbeg PA37 1QA, Argyll, Scotland.	Philip.kerrison@sams.ac.uk			Agritech Catalyst project Seaweed as a Solution for Sustainable Economic and Environmental Development (S3EED) [131598]; British CouncilThe British Council in India [127401486]; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/M005127/1] Funding Source: researchfish	Funding was provided by the Agritech Catalyst project Seaweed as a Solution for Sustainable Economic and Environmental Development (S<SUP>3</SUP>EED, grant 131598) to PK, GT, DS, RM, AH and a Researcher Links Travel Grant from the British Council (127401486) to HNL and PK. We thank Dr Alejandro Buschmann and two anonymous reviewers for valuable comments which improved this manuscript. We also thank CID Lines NV for providing us with samples for testing.	AGUIRRELIPPERHEIDE M, 1993, J PHYCOL, V29, P243, DOI 10.1111/j.0022-3646.1993.00243.x; Al-Hafedh YS, 2015, REV AQUACULT, V7, P161, DOI 10.1111/raq.12060; ANDERSON TW, 1952, ANN MATH STAT, V23, P193, DOI 10.1214/aoms/1177729437; AVENS AC, 1965, COMP BIOCHEM PHYSIOL, V16, P121, DOI 10.1016/0010-406X(65)90168-4; Baweja P, 2009, PHYCOL RES, V57, P45, DOI 10.1111/j.1440-1835.2008.00520.x; Bjork M, 2004, MAR ECOL PROG SER, V284, P109, DOI 10.3354/meps284109; Bolton JJ, 2009, J APPL PHYCOL, V21, P575, DOI 10.1007/s10811-008-9385-6; Borowitzka MA, 2007, ALGAL CULTURING TECH, P205; Bruhn A, 2011, BIORESOURCE TECHNOL, V102, P2595, DOI 10.1016/j.biortech.2010.10.010; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; COLORNI A, 1989, DIS AQUAT ORGAN, V7, P71, DOI 10.3354/dao007071; Cosgrove J, 2010, DEVEL APPL PHYCOL, V4, P1, DOI 10.1007/978-90-481-9268-7_1; DEBUSK TA, 1986, BOT MAR, V29, P381, DOI 10.1515/botm.1986.29.5.381; del Campo E, 1998, J PHYCOL, V34, P160, DOI 10.1046/j.1529-8817.1998.340160.x; Druehl L.D., 1969, PHYCOLOGIA, V8, P47, DOI 10.2216/i0031-8884-8-1-47.1; Dytham C., 2003, CHOOSING USING STAT, P248; Edwards MD, 2011, AQUACULTURE, V317, P203, DOI 10.1016/j.aquaculture.2011.04.007; Enriquez S, 2010, DEVEL APPL PHYCOL, V4, P187, DOI 10.1007/978-90-481-9268-7_9; Falkowski P., 2007, AQUATIC PHOTOSYNTHES, V2nd edn, P484; FAO, 2012, STAT WORLD FISH AQ 2; Fernandes DRP, 2011, REV BRAS FARMACOGN, V21, P313, DOI 10.1590/S0102-695X2011005000063; Figueroa FL, 2006, BOT MAR, V49, P275, DOI 10.1515/BOT.2006.035; Flameling IA, 1998, LIMNOL OCEANOGR, V43, P284, DOI 10.4319/lo.1998.43.2.0284; FRIES L, 1980, J PHYCOL, V16, P475; FRIES L, 1977, PHYCOLOGIA, V16, P451, DOI 10.2216/i0031-8884-16-4-451.1; Gibor A, 1981, P INT SEAW S, V10, P587; Gross EM, 2003, CRIT REV PLANT SCI, V22, P313, DOI 10.1080/713610859; Guillard RRL, 2007, ALGAL CULTURING TECH, P117; Hiraoka M, 2008, J APPL PHYCOL, V20, P97, DOI 10.1007/s10811-007-9186-3; Hoshaw RW, 1973, HDB PHYCOLOGICAL MET, P53; HSIAO SIC, 1971, CAN J BOTANY, V49, P1503, DOI 10.1139/b71-211; Hughes AD, 2013, MAR POLICY, V38, P554, DOI 10.1016/j.marpol.2012.08.001; Hwang Eun Kyoung, 2006, Algae, V21, P317; Kawachi M, 2005, ALGAL CULTURING TECH, P65; Kawai H., 2007, ALGAL CULTURING TECH, P133; KAWASHIMA Y, 1990, HYDROBIOLOGIA, V204, P375, DOI 10.1007/BF00040259; Kerrison PD, 2016, J APPL PHYCOL, V28, P1169, DOI 10.1007/s10811-015-0672-8; Kientz B, 2011, BOT MAR, V54, P457, DOI 10.1515/BOT.2011.053; KOLBER Z, 1993, LIMNOL OCEANOGR, V38, P1646, DOI 10.4319/lo.1993.38.8.1646; LAWLOR HJ, 1991, BOT MAR, V34, P261, DOI 10.1515/botm.1991.34.3.261; Le Gall L, 2004, AQUACULTURE, V229, P181, DOI 10.1016/S0044-8486(03)00390-9; LEE TF, 1985, BOT MAR, V28, P179, DOI 10.1515/botm.1985.28.5.179; Levene H., 1960, CONTRIBUTIONS PROBAB, P278; Luning K, 2003, J APPL PHYCOL, V15, P115, DOI 10.1023/A:1023807503255; Martinez B, 2006, AQUACULTURE, V254, P376, DOI 10.1016/j.aquaculture.2005.10.025; Mata L, 2016, J APPL PHYCOL, V28, P365, DOI 10.1007/s10811-015-0561-1; Maxwell K, 2000, J EXP BOT, V51, P659, DOI 10.1093/jexbot/51.345.659; McAllen R, 1999, J MAR BIOL ASSOC UK, V79, P1125, DOI 10.1017/S0025315499001393; McCracken IR, 1989, P NS I SCI, V38, P145; McMahon Robert F., 2003, P131; MORGAN KC, 1981, BOT MAR, V24, P547, DOI 10.1515/botm.1981.24.10.547; Msuya FE, 2006, AQUACULTURE, V254, P284, DOI 10.1016/j.aquaculture.2005.10.044; Msuya FE, 2010, J PHYCOL, V46, P813, DOI 10.1111/j.1529-8817.2010.00843.x; NEISH AC, 1977, CAN J BOT, V55, P2263, DOI 10.1139/b77-256; Neori A, 2003, J APPL PHYCOL, V15, P543, DOI 10.1023/B:JAPH.0000004382.89142.2d; Ohno M, 2006, ADV SEAWEED CULTIVAT, P1; Pang SJ, 2007, J APPL PHYCOL, V19, P557, DOI 10.1007/s10811-007-9170-y; Rego A, 2014, AQ EUR DON SAN SEB S; Richmond A., 2004, Handbook of microalgal culture: biotechnology and applied phycology, P125; Rod K. K, 2012, SORI DISINFECTION CU; RUMSEY TJ, 1973, COMP BIOCHEM PHYSIOL, V45, P327, DOI 10.1016/0300-9629(73)90439-8; SCHREIBER U, 1986, PHOTOSYNTH RES, V10, P51, DOI 10.1007/BF00024185; Shephard DC, 1970, METHOD CELL PHYSIOL, V4, P46; Sokal RR, 1995, FREEMAN, V3, P1995, DOI DOI 10.1016/J.JIP.2003.08.007; Tamiya H, 1955, WORLD S APPL SOL EN, P231; TORGERSEN Y, 1995, REV SCI TECH OIE, V14, P419, DOI 10.20506/rst.14.2.845; Vadas RL, 1977, ERDA S SERIES, P202; YAN ZM, 1984, HYDROBIOLOGIA, V116, P314	68	2	2	0	22	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0921-8971	1573-5176		J APPL PHYCOL	J. Appl. Phycol.	DEC	2016	28	6					3423	3434		10.1007/s10811-016-0873-9			12	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	EF1NA	WOS:000390090300027	28035174	Green Published, Other Gold			2021-04-07	
J	Qian, WG; Li, N; Lin, LD; Xu, T; Zhang, X; Wang, LH; Zou, HX; Wu, MJ; Yan, XF				Qian, Wei-Guo; Li, Nan; Lin, Li-Dong; Xu, Tao; Zhang, Xu; Wang, Li-Hua; Zou, Hui-Xi; Wu, Ming-Jiang; Yan, Xiu-Feng			Parallel analysis of proteins in brown seaweed Sargassum fusiforme responding to hyposalinity stress	AQUACULTURE			English	Article						Hyposalinity stress; Proteome; Sargassum fusiforme; Phaeophyceae	ALGA ECTOCARPUS-SILICULOSUS; VESICULOSUS L. PHAEOPHYCEAE; FUCUS-VESICULOSUS; EXPRESSION ANALYSIS; ATP SYNTHASE; MARINE; GENES; REVEALS; SUBUNIT; LIGHT	The intertidal seaweed Sargassum fusiforme shows remarkable tolerances to freshwater immersion, which made experienced cultivators quite convenient to control epiphytic algae that would limit the massive aquaculture production. Here, we performed biochemical and physiological analyses and comparative proteomics to investigate the molecular mechanisms of the hyposalinity-stress-tolerance in S. fusiforme. Our results showed that in the initial period of freshwater immersion, mineral fraction and inorganic anion may be lost much faster than other soluble contents. H2O2 accumulation occurred immediately and the antioxidant system was efficiently activated during freshwater immersion. Comparative proteomic analysis revealed 51 differentially expressed protein spots in S. fusiforme, most of which were enzymes involved in photosynthesis, carbohydrate metabolism and energy metabolism. The one-hour's-freshwater-immersion negatively affected the metabolic activity of S. fusiforme, as synthesis of most key metabolic enzymes were inhibited in varying degrees, with only a few exceptions, e.g., phosphomannomutase, which is related to precursor synthesis of cell wall polysaccharides. Statement of relevance: In order to eliminate other wild harmful algae, freshwater or lower salinity of seawater is usually employed to the brown alga Sargassum fusiforme for a short period, indicating its tolerance to hyposalinity stress. Osmotic stress is one of the major abiotic stresses for most algae. As most studies were concerning about the hypersalinity stress, physiological mechanisms of tolerance to hyposalinity stress of algae are not fully understood. This study focuses on responses of the brown alga S. fusiforme to short-term(or acute) hyposalinity stress, using comparative physiology approach combining methods of systems biology including proteomics. Then analyzes the typical physiological characteristics related to osmotic stress and overall physiological metabolic networks to investigate the possible tolerance mechanism of S. fusiforme to hyposalinity stress at physiological level. As an important foundation work, analyzing the effect of hyposalinity stress on the survival of S. fusiforme and its mechanism is helpful to explore the tolerance of algae to abiotic stress. In another aspect, it will also provide the necessary theoretical basis for the freshwater pesticide technology. (C) 2016 Elsevier B.V. All rights reserved.	[Qian, Wei-Guo; Li, Nan; Zhang, Xu; Wang, Li-Hua; Zou, Hui-Xi; Wu, Ming-Jiang; Yan, Xiu-Feng] Wenzhou Univ, Coll Life & Environm Sci, Prov Wenzhou Key Lab Water Environm & Marine Biol, Wenzhou 325035, Peoples R China; [Qian, Wei-Guo; Xu, Tao] Northeast Forest Univ, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Harbin 150040, Peoples R China; [Lin, Li-Dong] Inst Dongtou Fisheries Sci & Technol Res, Wenzhou 325000, Peoples R China	Zou, HX; Wu, MJ (corresponding author), Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325035, Zhejiang, Peoples R China.	zjuzhx@wzu.edu.cn; wumingjiangwz@163.com		Zou, Hui-Xi/0000-0002-8141-8059; Yan, Xiufeng/0000-0001-5127-494X	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31400334, 31270541, 31200266]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LQ13C030005]	This work was supported by funding from the National Natural Science Foundation of China (31400334, 31270541 and 31200266) and the Zhejiang Provincial Natural Science Foundation of China (LQ13C030005).	APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Asada K., 1987, PHOTOINHIBITION, P228; BEAUCHAM.C, 1971, ANAL BIOCHEM, V44, P276, DOI 10.1016/0003-2697(71)90370-8; Beilby MJ, 2006, PLANT CELL ENVIRON, V29, P764, DOI 10.1111/j.1365-3040.2005.01437.x; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 1999, J PHYCOL, V35, P62, DOI 10.1046/j.1529-8817.1999.3510062.x; De Martino A, 2000, EUR J BIOCHEM, V267, P5540, DOI 10.1046/j.1432-1327.2000.01616.x; Desimone M, 1996, PLANT PHYSIOL, V111, P789, DOI 10.1104/pp.111.3.789; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Feng YJ, 2014, ACTA OCEANOL SIN, V33, P108, DOI 10.1007/s13131-014-0447-0; GAGNE G, 1992, PLANT MOL BIOL, V18, P429, DOI 10.1007/BF00040659; Gomez-Ordonez E, 2010, TALANTA, V82, P1313, DOI 10.1016/j.talanta.2010.06.062; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Gylle AM, 2013, HYDROBIOLOGIA, V700, P109, DOI 10.1007/s10750-012-1231-9; Gylle AM, 2009, PHYCOL RES, V57, P127, DOI 10.1111/j.1440-1835.2009.00529.x; Gylle AM, 2009, PHYCOLOGIA, V48, P156, DOI 10.2216/08-45.1; He ZY, 2004, PLANT PHYSIOL, V134, P1248, DOI 10.1104/pp.103.031005; Horwich AL, 2007, ANNU REV CELL DEV BI, V23, P115, DOI 10.1146/annurev.cellbio.23.090506.123555; Ichihara K, 2011, J PHYCOL, V47, P584, DOI 10.1111/j.1529-8817.2011.01001.x; Karsten U., 2012, ECOLOGICAL STUDIES S, P87, DOI [10.1007/978-3-642-28451-9_5, DOI 10.1007/978-3-642-28451-9_5]; Kiang JG, 1998, PHARMACOL THERAPEUT, V80, P183, DOI 10.1016/S0163-7258(98)00028-X; Kumar M, 2014, ADV BOT RES, V71, P91, DOI 10.1016/B978-0-12-408062-1.00004-4; Kumari S, 2013, PLANT SIGNAL BEHAV, V8, DOI 10.4161/psb.22734; Llorca O, 2000, EMBO J, V19, P5971, DOI 10.1093/emboj/19.22.5971; Lurie S, 1997, PHYSIOL MOL PLANT P, V50, P141, DOI 10.1006/pmpp.1996.0074; MABEAU S, 1987, J EXP BOT, V38, P1573, DOI 10.1093/jxb/38.9.1573; Maegawa Y, 2006, ACTA CRYSTALLOGR D, V62, P483, DOI 10.1107/S0907444906006329; McCarty RE, 2000, ANNU REV PLANT PHYS, V51, P83, DOI 10.1146/annurev.arplant.51.1.83; McKew BA, 2013, NEW PHYTOL, V200, P61, DOI 10.1111/nph.12352; Meslet-Cladiere L, 2013, PLANT CELL, V25, P3089, DOI 10.1105/tpc.113.111336; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Morris CA, 2014, PLANT CELL ENVIRON, V37, P189, DOI 10.1111/pce.12145; OKAZAKI Y, 1992, PLANT CELL ENVIRON, V15, P61, DOI 10.1111/j.1365-3040.1992.tb01458.x; Pang SJ, 2008, AQUAC RES, V39, P1408, DOI 10.1111/j.1365-2109.2008.02010.x; Qian WQ, 2007, PLANT J, V49, P399, DOI 10.1111/j.1365-313X.2006.02967.x; Romero-Puertas MC, 2004, PLANT CELL ENVIRON, V27, P1122, DOI 10.1111/j.1365-3040.2004.01217.x; Ruperez P, 2002, FOOD CHEM, V79, P23, DOI 10.1016/S0308-8146(02)00171-1; Savard F, 1996, PLANT MOL BIOL, V32, P461, DOI 10.1007/BF00019098; Shabala L, 2009, ENVIRON MICROBIOL, V11, P1835, DOI 10.1111/j.1462-2920.2009.01908.x; Sheffield J, 2006, PROTEOMICS, V6, P1588, DOI 10.1002/pmic.200500503; Teo SS, 2009, J PHYCOL, V45, P1093, DOI 10.1111/j.1529-8817.2009.00724.x; Wang YH, 2002, PLANT PHYSIOL, V130, P1361, DOI 10.1104/pp.008854; Xu J, 2012, PLOS ONE, V7, DOI [10.1371/journal.pone.0029552, 10.1371/journal.pone.0048140]; Xu T, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0105768; Zhang AQ, 2015, AQUAT TOXICOL, V163, P1, DOI 10.1016/j.aquatox.2015.03.018; Zhu S.H., 2008, PHOTOSYNTHESIS ENERG, P261; Zou HX, 2015, ECOTOX ENVIRON SAFE, V111, P271, DOI 10.1016/j.ecoenv.2014.10.028; Zou HX, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0101960	50	7	7	1	52	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0044-8486	1873-5622		AQUACULTURE	Aquaculture	DEC 1	2016	465						189	197		10.1016/j.aquaculture.2016.08.032			9	Fisheries; Marine & Freshwater Biology	Fisheries; Marine & Freshwater Biology	DY8IE	WOS:000385372200026					2021-04-07	
J	Fischl, R; Bertelsen, K; Gaillard, F; Coelho, S; Michel, G; Klinger, M; Boyen, C; Czjzek, M; Herve, C				Fischl, Richard; Bertelsen, Kresten; Gaillard, Fanny; Coelho, Susana; Michel, Gurvan; Klinger, Markus; Boyen, Catherine; Czjzek, Mirjam; Herve, Cecile			The cell-wall active mannuronan C5-epimerases in the model brown alga Ectocarpus: From gene context to recombinant protein	GLYCOBIOLOGY			English	Article						alginate; brown algae; Ectocarpus; mannuronan C5-epimerase	C-5 EPIMERASES; ALGINATE; EXPRESSION; POLYSACCHARIDES; EPIMERIZATION; SPECTROSCOPY; MECHANISM; EVOLUTION; SEQUENCE; ACID	Mannuronan C5-epimerases (ManC5-Es) catalyze in brown algae the remodeling of alginate, a major cell-wall component which is involved in many biological functions in these organisms. ManC5-Es are present as large multigenic families in brown algae, likely indicating functional specificities and specializations. ManC5-Es control the distribution pattern of (1-4) linked beta-d-mannuronic acid (M) and alpha-l-guluronic acid (G) residues in alginates, giving rise to widely different polysaccharide compositions and sequences, depending on tissue, season, age, or algal species. As such they are also a source of powerful new tools for the biotechnological and enzymatic processing of alginates, to match the growing interest for food hydrocolloids and in biomedical and nanotechnological applications. We report here the first heterologous production of a ManC5-E of brown algal origin that is successfully refolded in an active form. The activity was measured by H-1 NMR and by an indirect enzymatic assay using a known bacterial alginate lyase. The transcript expression as a function of the developmental program of the brown alga Ectocarpus, together with the bioinformatic analyses of the corresponding gene context of this multigenic family, is also presented.	[Fischl, Richard; Coelho, Susana; Michel, Gurvan; Boyen, Catherine; Czjzek, Mirjam; Herve, Cecile] Univ Paris 06, Univ Paris 04, CS 90074, Roscoff, France; [Fischl, Richard; Coelho, Susana; Michel, Gurvan; Boyen, Catherine; Czjzek, Mirjam; Herve, Cecile] Stn Biol Roscoff, CNRS, UMR 8227, Integrat Biol Marine Models, CS 90074, Roscoff, France; [Bertelsen, Kresten; Klinger, Markus] DuPont Nutr Biosci ApS, Aarhus, Denmark; [Gaillard, Fanny] Stn Biol Roscoff, FR2424, CS 90074, Roscoff, France; [Klinger, Markus] Novozymes AS, Bagsvaerd, Denmark	Czjzek, M; Herve, C (corresponding author), Univ Paris 06, Univ Paris 04, CS 90074, Roscoff, France.; Czjzek, M; Herve, C (corresponding author), Stn Biol Roscoff, CNRS, UMR 8227, Integrat Biol Marine Models, CS 90074, Roscoff, France.	mirjam.czjzek@sb-roscoff.fr; cecile.herve@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020	Herve, Cecile/0000-0001-6649-8137; MICHEL, Gurvan/0000-0002-3009-6205	European Community's Seventh Framework Programme (FP7) [222628]; Region BretagneRegion Bretagne; Centre National de la Recherche Scientifique (CNRS)Centre National de la Recherche Scientifique (CNRS)	The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under Grant Agreement No. 222628 (www.polymode.eu). This work was also supported by the Region Bretagne and the Centre National de la Recherche Scientifique (CNRS).	Bjerkan TM, 2004, BIOCHEM J, V381, P813, DOI 10.1042/BJ20031580; Buchinger E, 2014, J BIOL CHEM, V289, P31382, DOI 10.1074/jbc.M114.567008; Campa C, 2004, BIOCHEM J, V381, P155, DOI 10.1042/BJ20031265; Cock JM, 2015, ADV MAR GENOMICS, V2, P153, DOI 10.1007/978-94-017-9642-2_8; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Donati I, 2005, BIOMACROMOLECULES, V6, P1031, DOI 10.1021/bm049306e; Draget KI, 2011, FOOD HYDROCOLLOID, V25, P251, DOI 10.1016/j.foodhyd.2009.10.007; Enquist-Newman M, 2014, NATURE, V505, P239, DOI 10.1038/nature12771; Ertesvag H, 2015, FRONT MICROBIOL, V6, DOI 10.3389/fmicb.2015.00523; GRANT GT, 1973, FEBS LETT, V32, P195, DOI 10.1016/0014-5793(73)80770-7; GRASDALEN H, 1981, CARBOHYD RES, V89, P179, DOI 10.1016/S0008-6215(00)85243-X; GRASDALEN H, 1983, CARBOHYD RES, V118, P255, DOI 10.1016/0008-6215(83)88053-7; Groisillier A, 2010, MICROB CELL FACT, V9, DOI 10.1186/1475-2859-9-45; Hartmann M, 2002, BIOPOLYMERS, V63, P77, DOI 10.1002/bip.10017; HAUG A, 1974, CARBOHYD RES, V32, P217, DOI 10.1016/S0008-6215(00)82100-X; Helmus JJ, 2013, J BIOMOL NMR, V55, P355, DOI 10.1007/s10858-013-9718-x; Herve C, 2016, NEW PHYTOL, V209, P1428, DOI 10.1111/nph.13786; Heyraud A, 1996, CARBOHYD RES, V289, P11, DOI 10.1016/0008-6215(96)00060-2; Korf U, 2005, PROTEOMICS, V5, P3571, DOI 10.1002/pmic.200401195; Kovalenko I, 2011, SCIENCE, V334, P75, DOI 10.1126/science.1209150; Kurland C, 1996, CURR OPIN BIOTECH, V7, P489, DOI 10.1016/S0958-1669(96)80050-4; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Micheli F, 2001, TRENDS PLANT SCI, V6, P414, DOI 10.1016/S1360-1385(01)02045-3; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Pawar SN, 2012, BIOMATERIALS, V33, P3279, DOI 10.1016/j.biomaterials.2012.01.007; Robert X, 2014, NUCLEIC ACIDS RES, V42, pW320, DOI 10.1093/nar/gku316; Rozeboom HJ, 2008, J BIOL CHEM, V283, P23819, DOI 10.1074/jbc.M804119200; Sambrook J, 2001, MOL CLONING LAB MANU, P999, DOI DOI 10.1016/0092-8674(90)90210-6; SKJAK-BRAEK G, 2005, POLYSACCHARIDES POLY; SMIDSR OD, 1996, CARBOHYDR EUR, V14, P6; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Thomas F, 2013, J BIOL CHEM, V288, P23021, DOI 10.1074/jbc.M113.467217; Tondervik A, 2013, BIOMACROMOLECULES, V14, P2657, DOI 10.1021/bm4005194; Tonon T, 2008, J PHYCOL, V44, P1250, DOI 10.1111/j.1529-8817.2008.00580.x; Wargacki AJ, 2012, SCIENCE, V335, P308, DOI 10.1126/science.1214547; Wei N, 2013, TRENDS BIOTECHNOL, V31, P70, DOI 10.1016/j.tibtech.2012.10.009; Wolfram F, 2014, J BIOL CHEM, V289, P6006, DOI 10.1074/jbc.M113.533158; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Zhao D, 2012, MOL BIOSYST, V8, P753, DOI 10.1039/c1mb05337j	40	12	12	2	36	OXFORD UNIV PRESS INC	CARY	JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA	0959-6658	1460-2423		GLYCOBIOLOGY	Glycobiology	SEP	2016	26	9					973	983		10.1093/glycob/cww040			11	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	DZ7MH	WOS:000386049500009	27026155	Bronze			2021-04-07	
J	Rousvoal, S; Bouyer, B; Lopez-Cristoffanini, C; Boyen, C; Collen, J				Rousvoal, Sylvie; Bouyer, Betty; Lopez-Cristoffanini, Camilo; Boyen, Catherine; Collen, Jonas			Mutant swarms of a totivirus-like entities are present in the red macroalga Chondrus crispus and have been partially transferred to the nuclear genome	JOURNAL OF PHYCOLOGY			English	Article						Chondrus; dsRNA; mutant swarm; Rhodophyta; totivirus; virus	DOUBLE-STRANDED-RNA; VIRUS-LIKE PARTICLES; HORIZONTAL GENE-TRANSFER; E-BOX ELEMENT; MOLECULAR CHARACTERIZATION; ECTOCARPUS-SILICULOSUS; GREEN-ALGA; DNA VIRUS; GCC-BOX; MARINE VIRUSES	Chondrus crispus Stackhouse (Gigartinales) is a red seaweed found on North Atlantic rocky shores. Electrophoresis of RNA extracts showed a prominent band with a size of around 6,000bp. Sequencing of the band revealed several sequences with similarity to totiviruses, double-stranded RNA viruses that normally infect fungi. This virus-like entity was named C.crispus virus (CcV). It should probably be regarded as an extreme viral quasispecies or a mutant swarm since low identity (<65%) was found between sequences. Totiviruses typically code for two genes: one capsid gene (gag) and one RNA-dependent RNA polymerase gene (pol) with a pseudoknot structure between the genes. Both the genes and the intergenic structures were found in the CcV sequences. A nonidentical gag gene was also found in the nuclear genome of C.crispus, with associated expressed sequence tags (EST) and upstream regulatory features. The gene was presumably horizontally transferred from the virus to the alga. Similar dsRNA bands were seen in extracts from different life cycle stages of C.crispus and from all geographic locations tested. In addition, similar bands were also observed in RNA extractions from other red algae; however, the significance of this apparently widespread phenomenon is unknown. Neither phenotype caused by the infection nor any virus particles or capsid proteins were identified; thus, the presence of viral particles has not been validated. These findings increase the known host range of totiviruses to include marine red algae.	[Collen, Jonas] CNRS, UMR 8227, Integrat Biol Marine Models, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, UMR 8227,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; [Lopez-Cristoffanini, Camilo] Univ Barcelona, Dept Biol Vegetal, E-08028 Barcelona, Spain	Collen, J (corresponding author), CNRS, UMR 8227, Integrat Biol Marine Models, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.	collen@sb-roscoff.fr	Lopez-Cristoffanini, Camilo/B-5860-2017	Lopez-Cristoffanini, Camilo/0000-0002-2481-6122	Centre National de Recherche Scientifique; University Pierre and Marie Curie; IDEALG grants "Investissements d'avenir, Biotechnologies-Bioressources"French National Research Agency (ANR) [ANR-10-BTBR-04-02, ANR-10-BTBR-04-04]	Work at the Station Biologique de Roscoff was supported by the Centre National de Recherche Scientifique, the University Pierre and Marie Curie, and IDEALG grants ANR-10-BTBR-04-02 and ANR-10-BTBR-04-04 "Investissements d'avenir, Biotechnologies-Bioressources." We also want to thank Marion Azidrou and Lea Cabioch for work performed early in the project, Anne-Claire Baudoux for expertise on viral particle precipitation, Gabriel Markov for CYP analysis, Simon Dittami for helpful comments, and Gunilla Toth and Isabel Sousa Pinto for collecting C. crispus in Sweden and Portugal.	Allen A, 2011, PLANT BIOTECHNOL J, V9, P857, DOI 10.1111/j.1467-7652.2011.00590.x; Andino R, 2015, VIROLOGY, V479, P46, DOI 10.1016/j.virol.2015.03.022; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; APT KE, 1991, J PHYCOL, V27, P409, DOI 10.1111/j.0022-3646.1991.00409.x; Baeza M, 2012, VIROL J, V9, DOI 10.1186/1743-422X-9-140; Bekaert M, 2003, BIOINFORMATICS, V19, P327, DOI 10.1093/bioinformatics/btf868; Bidou L, 1997, RNA, V3, P1153; Brown RL, 2003, PLANT PHYSIOL, V132, P1020, DOI 10.1104/pp.102.017814; BRUENN JA, 1993, NUCLEIC ACIDS RES, V21, P5667, DOI 10.1093/nar/21.24.5667; Brussaard CPD, 2004, VIROLOGY, V319, P280, DOI 10.1016/j.virol.2003.10.033; Buttner M, 1997, P NATL ACAD SCI USA, V94, P5961, DOI 10.1073/pnas.94.11.5961; Byun Y, 2009, BIOINFORMATICS, V25, P1435, DOI 10.1093/bioinformatics/btp252; Cao S, 2006, NUCLEIC ACIDS RES, V34, P2634, DOI 10.1093/nar/gkl346; Cao S, 2009, RNA, V15, P696, DOI 10.1261/rna.1429009; Chiba S, 2011, PLOS PATHOG, V7, DOI 10.1371/journal.ppat.1002146; Ciota AT, 2011, INFECT GENET EVOL, V11, P460, DOI 10.1016/j.meegid.2010.12.007; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2014, ADV BOT RES, V71, P53, DOI 10.1016/B978-0-12-408062-1.00003-2; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; Crochu S, 2004, J GEN VIROL, V85, P1971, DOI 10.1099/vir.0.79850-0; Delaroque N, 2003, J MOL EVOL, V57, P613, DOI 10.1007/s00239-003-2501-y; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Derelle E, 2015, J VIROL, V89, P5812, DOI 10.1128/JVI.00246-15; Derelle E, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002250; Djikeng A, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-5; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; Fuhrman JA, 1999, NATURE, V399, P541, DOI 10.1038/21119; Ghabrial SA, 2009, ANNU REV PHYTOPATHOL, V47, P353, DOI 10.1146/annurev-phyto-080508-081932; Giedroc DP, 2000, J MOL BIOL, V298, P167, DOI 10.1006/jmbi.2000.3668; GIULIANO G, 1988, P NATL ACAD SCI USA, V85, P7089, DOI 10.1073/pnas.85.19.7089; Goker M, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0022252; Haugland O, 2011, J VIROL, V85, P5275, DOI 10.1128/JVI.02154-10; Holmes EC, 2011, CELL HOST MICROBE, V10, P368, DOI 10.1016/j.chom.2011.09.002; Isawa H, 2011, VIRUS RES, V155, P147, DOI 10.1016/j.virusres.2010.09.013; ISHIHARA J, 1992, PLANTA, V187, P475, DOI 10.1007/BF00199965; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; JACKS T, 1988, CELL, V55, P447, DOI 10.1016/0092-8674(88)90031-1; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; Katzourakis A, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1001191; Koga R, 1998, PLANT MOL BIOL, V36, P717, DOI 10.1023/A:1005907310553; Koga R, 2003, PLANT MOL BIOL, V51, P991, DOI 10.1023/A:1023003412859; KOONIN EV, 1991, VIRUS GENES, V5, P273, DOI 10.1007/BF00568977; LACLAIRE JW, 1977, PROTOPLASMA, V93, P127, DOI 10.1007/BF01276287; Lang AS, 2009, FEMS MICROBIOL REV, V33, P295, DOI 10.1111/j.1574-6976.2008.00132.x; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LEE RE, 1971, J CELL SCI, V8, P623; LEGALL Y, 1990, PLANT CELL REP, V8, P582, DOI 10.1007/BF00270058; Liu HQ, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0042147; Liu HQ, 2012, BMC EVOL BIOL, V12, DOI 10.1186/1471-2148-12-91; Liu HQ, 2010, J VIROL, V84, P11876, DOI 10.1128/JVI.00955-10; Magliani W, 1997, CLIN MICROBIOL REV, V10, P369, DOI 10.1128/CMR.10.3.369; Maier I, 1998, EUR J PHYCOL, V33, P213, DOI 10.1017/S0967026298001747; Moreau H, 2010, J VIROL, V84, P12555, DOI 10.1128/JVI.01123-10; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 2000, BOT MAR, V43, P157, DOI 10.1515/BOT.2000.016; MULLER DG, 1993, HYDROBIOLOGIA, V261, P37; Nibert ML, 2007, J GEN VIROL, V88, P1315, DOI 10.1099/vir.0.82681-0; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; Pearson MN, 2009, MOL PLANT PATHOL, V10, P115, DOI 10.1111/j.1364-3703.2008.00503.x; Philippe N, 2013, SCIENCE, V341, P281, DOI 10.1126/science.1239181; Pueschel CM, 1995, CAN J BOT, V73, P1974, DOI 10.1139/b95-211; Reinhold H, 2011, PLANT CELL, V23, P1391, DOI 10.1105/tpc.110.081950; REISSER W, 1993, ARCH PROTISTENKD, V143, P257, DOI 10.1016/S0003-9365(11)80293-9; Ribas JC, 1998, J BIOL CHEM, V273, P9306, DOI 10.1074/jbc.273.15.9306; Roossinck MJ, 2010, PHILOS T R SOC B, V365, P1899, DOI 10.1098/rstb.2010.0057; Routhier E, 1998, J VIROL, V72, P4427, DOI 10.1128/JVI.72.5.4427-4429.1998; Sambrook Joseph, 2006, CSH Protoc, V2006, DOI [10.1101/pdb.prot4022, 10.1101/pdb.prot3825, 10.1101/pdb.prot3966, 10.1101/pdb.prot4085, 10.1101/pdb.prot3871, 10.1101/pdb.prot4044, 10.1101/pdb.prot3972, 10.1101/pdb.prot3723, 10.1101/pdb.prot4455, 10.1101/pdb.prot4027, 10.1101/pdb.prot4453, 10.1101/pdb.prot4050, 10.1101/pdb.prot4056]; Schroeder D. C., 2011, STUDIES VIRAL ECOLOG, V1, P205; Seitz SB, 2010, PLANT SIGNAL BEHAV, V5, P1077, DOI 10.4161/psb.5.9.12564; Seitz SB, 2010, PLANT PHYSIOL, V152, P2243, DOI 10.1104/pp.109.149195; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; Solano R, 1998, GENE DEV, V12, P3703, DOI 10.1101/gad.12.23.3703; STAIGER D, 1989, P NATL ACAD SCI USA, V86, P6930, DOI 10.1073/pnas.86.18.6930; Suttle CA, 2005, NATURE, V437, P356, DOI 10.1038/nature04160; Suttle CA, 2007, NAT REV MICROBIOL, V5, P801, DOI 10.1038/nrmicro1750; Tournier B, 2003, FEBS LETT, V550, P149, DOI 10.1016/S0014-5793(03)00757-9; TRIPODI G, 1976, ARCH MICROBIOL, V108, P167, DOI 10.1007/BF00428947; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; Vignuzzi M, 2006, NATURE, V439, P344, DOI 10.1038/nature04388; West JA, 2013, ALGAE-SEOUL, V28, P83, DOI 10.4490/algae.2013.28.1.083; WILLIAMS ME, 1992, PLANT CELL, V4, P485, DOI 10.1105/tpc.4.4.485; Wu QF, 2010, P NATL ACAD SCI USA, V107, P1606, DOI 10.1073/pnas.0911353107; Zhai Y, 2010, J GEN VIROL, V91, P2836, DOI 10.1099/vir.0.024794-0; Zwart MP, 2014, MOL BIOL EVOL, V31, P121, DOI 10.1093/molbev/mst175	84	3	3	0	12	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	AUG	2016	52	4					493	504		10.1111/jpy.12427			12	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	DW4CM	WOS:000383589700002	27151076				2021-04-07	
J	Terauchi, M; Nagasato, C; Inoue, A; Ito, T; Motomura, T				Terauchi, Makoto; Nagasato, Chikako; Inoue, Akira; Ito, Toshiaki; Motomura, Taizo			Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae)	PLANTA			English	Article						Calcium; Cell wall integrity; Electron microscopy; Electron tomography; Proteomics	DIRECT VISUALIZATION; PHYSICAL-PROPERTIES; LOCALIZATION; POLYSACCHARIDES; SCYTOSIPHONALES; CYTOKINESIS; PROTEINS; ZYGOTES; PECTIN; MATRIX	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall. Calcium greatly contributes to the cell wall integrity. Brown algae have a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides. However, the relationship between the architecture and the composition of the cell wall is poorly understood. Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies. The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils. Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall. By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen. Destruction of cell wall integrity was also induced by the addition of sorbitol. The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils. It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall. This study provides insights into the design principle of the brown algal cell wall.	[Terauchi, Makoto] Hokkaido Univ, Grad Sch Environm Sci, Sapporo, Hokkaido 0600810, Japan; [Terauchi, Makoto] Kobe Univ, Res Ctr Inland Seas, Kobe, Hyogo 6578501, Japan; [Nagasato, Chikako; Motomura, Taizo] Hokkaido Univ, Muroran Marine Stn, Field Sci Ctr Northern Biosphere, Muroran, Hokkaido 0510013, Japan; [Inoue, Akira] Hokkaido Univ, Grad Sch Fisheries Sci, Hakodate, Hokkaido 0418611, Japan; [Ito, Toshiaki] Hokkaido Univ, Res Fac Agr, Electron Microscope Lab, Sapporo, Hokkaido 0608589, Japan	Nagasato, C (corresponding author), Hokkaido Univ, Muroran Marine Stn, Field Sci Ctr Northern Biosphere, Muroran, Hokkaido 0510013, Japan.	nagasato@fsc.hokudai.ac.jp			KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [25291087, 26440160]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [26440160] Funding Source: KAKEN	We express our thanks to Dr. K. Okuda, Kochi University, Japan, for providing the anti-alginate antibody, and Dr. S. Oka, Hokkaido University, Japan, for analysis with the LC-MS/MS. This study was supported by KAKENHI [Grant numbers 25291087, 26440160].	Abramoff MD., 2004, BIOPHOTONICS INT, V11, P36, DOI 10.3233/ISU-1991-115-601.; Agrawal GK, 2010, PROTEOMICS, V10, P799, DOI 10.1002/pmic.200900514; Aithal A, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0034613; Albenne C, 2014, PROTEOMES, V2, DOI 10.3390/proteomes2020224; Baskin TI, 2005, ANNU REV CELL DEV BI, V21, P203, DOI 10.1146/annurev.cellbio.20.082503.103053; Bisgrove SR, 2001, PLANTA, V212, P648, DOI 10.1007/s004250000434; BURNS AR, 1982, NEW PHYTOL, V92, P297, DOI 10.1111/j.1469-8137.1982.tb03388.x; CALLOW ME, 1978, J CELL SCI, V32, P337; CARPITA NC, 1993, PLANT J, V3, P1, DOI 10.1111/j.1365-313X.1993.tb00007.x; Chi Eun-Sup, 1999, Phycological Research, V47, P53, DOI 10.1046/j.1440-1835.1999.00151.x; CRONSHAW J, 1958, BIOCHIM BIOPHYS ACTA, V27, P89, DOI 10.1016/0006-3002(58)90295-6; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; EVANS LV, 1972, NEW PHYTOL, V71, P1161, DOI 10.1111/j.1469-8137.1972.tb01994.x; Fu G, 2014, PROTIST, V165, P662, DOI 10.1016/j.protis.2014.07.007; Fu G, 2013, PROTOPLASMA, V250, P261, DOI 10.1007/s00709-012-0405-7; GACESA P, 1990, APPL ENVIRON MICROB, V56, P2265, DOI 10.1128/AEM.56.7.2265-2267.1990; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; HAUG A, 1974, CARBOHYD RES, V32, P217, DOI 10.1016/S0008-6215(00)82100-X; Hepler PK, 2005, PLANT CELL, V17, P2142, DOI 10.1105/tpc.105.032508; Inoue A, 2014, MAR DRUGS, V12, P4693, DOI 10.3390/md12084693; Katsaros CI, 2002, PHYCOLOGIA, V41, P178, DOI 10.2216/i0031-8884-41-2-178.1; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; LYNCH MA, 1992, J CELL BIOL, V118, P467, DOI 10.1083/jcb.118.2.467; MABEAU S, 1987, J EXP BOT, V38, P1573, DOI 10.1093/jxb/38.9.1573; MARIANI P, 1985, PROTOPLASMA, V128, P208, DOI 10.1007/BF01276343; MCCANN MC, 1990, J CELL SCI, V96, P323; Mohnen D, 2008, CURR OPIN PLANT BIOL, V11, P266, DOI 10.1016/j.pbi.2008.03.006; Morris VJ, 2009, STRUCT CHEM, V20, P255, DOI 10.1007/s11224-009-9410-7; Nagasato C, 2002, PROTOPLASMA, V219, P140, DOI 10.1007/s007090200015; Nagasato C, 2014, PROTOPLASMA, V251, P1347, DOI 10.1007/s00709-014-0635-y; Nagasato C, 2010, PLANTA, V232, P287, DOI 10.1007/s00425-010-1188-8; Nagasato C, 2009, J PHYCOL, V45, P404, DOI 10.1111/j.1529-8817.2009.00655.x; Nakashima J, 1997, PLANT CELL PHYSIOL, V38, P818, DOI 10.1093/oxfordjournals.pcp.a029240; NOVOTNY AM, 1975, PLANTA, V122, P67, DOI 10.1007/BF00385406; NOVOTNY AM, 1974, DEV BIOL, V40, P162, DOI 10.1016/0012-1606(74)90116-X; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; OSBORN M, 1982, METHOD CELL BIOL, V24, P97; Parre E, 2005, PLANTA, V220, P582, DOI 10.1007/s00425-004-1368-5; PICTON JM, 1983, PROTOPLASMA, V115, P11, DOI 10.1007/BF01293575; Provasoli L, 1968, CULTURES COLLECTIONS, P63; Provasoli L, 1963, P 4 INT SEAW S, P9; QUATRANO RS, 1976, PLANT PHYSIOL, V58, P224, DOI 10.1104/pp.58.2.224; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; RUEL K, 1984, HISTOCHEMISTRY, V81, P573, DOI 10.1007/BF00489537; Scheller HV, 2010, ANNU REV PLANT BIOL, V61, P263, DOI 10.1146/annurev-arplant-042809-112315; Schoenwaelder MEA, 1999, PHYCOLOGIA, V38, P161, DOI 10.2216/i0031-8884-38-3-161.1; Schuessler Arthur, 2003, Phycological Research, V51, P35; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; Tamura Hiroshi, 1996, Phycological Research, V44, P63, DOI 10.1111/j.1440-1835.1996.tb00039.x; Terauchi M, 2012, PLANTA, V236, P1013, DOI 10.1007/s00425-012-1656-4; WELLS B, 1994, J MICROSC-OXFORD, V173, P155, DOI 10.1111/j.1365-2818.1994.tb03438.x; Wu X, 2014, J APPL PHYCOL, V26, P2389, DOI 10.1007/s10811-014-0253-2; Xu P, 2007, WOOD SCI TECHNOL, V41, P101, DOI 10.1007/s00226-006-0088-3	54	9	9	0	37	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	0032-0935	1432-2048		PLANTA	Planta	AUG	2016	244	2					361	377		10.1007/s00425-016-2516-4			17	Plant Sciences	Plant Sciences	DR2MP	WOS:000379738900006	27072676				2021-04-07	
J	Mignerot, L; Coelho, SM				Mignerot, Laure; Coelho, Susana M.			The origin and evolution of the sexes: Novel insights from a distant eukaryotic linage	COMPTES RENDUS BIOLOGIES			English	Article						Evolution; Sex determination; Brown algae; Sex chromosomes; Reproductive system	BROWN ALGA ECTOCARPUS; CHROMOSOMES; GENOME; MULTICELLULARITY; IDENTIFICATION; REGIONS; LOCUS	Sexual reproduction is an extraordinarily widespread phenomenon that assures the production of new genetic combinations in nearly all eukaryotic lineages. Although the core features of sexual reproduction (meiosis and syngamy) are highly conserved, the control mechanisms that determine whether an individual is male or female are remarkably labile across eukaryotes. In genetically controlled sexual systems, gender is determined by sex chromosomes, which have emerged independently and repeatedly during evolution. Sex chromosomes have been studied in only a handful of classical model organism, and empirical knowledge on the origin and evolution of the sexes is still surprisingly incomplete. With the advent of new generation sequencing, the taxonomic breadth of model systems has been rapidly expanding, bringing new ideas and fresh views on this fundamental aspect of biology. This mini-review provides a quick state of the art of how the remarkable richness of the sexual characteristics of the brown algae is helping to increase our knowledge about the evolution of sex determination. (C) 2016 Academie des sciences. Published by Elsevier Masson SAS.	[Mignerot, Laure; Coelho, Susana M.] Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, Pl George Teissier,CS 90074, F-29688 Roscoff, France	Coelho, SM (corresponding author), Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, Pl George Teissier,CS 90074, F-29688 Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020		CNRSCentre National de la Recherche Scientifique (CNRS)European Commission; ANRFrench National Research Agency (ANR) [ANR-10-BLAN-1727, ANR-10-BTBR-04-01]; Interreg program France (Channel)-England (project Marinexus); UPMC; European Research CouncilEuropean Research Council (ERC)European Commission [638240]; Region BretagneRegion Bretagne	Work in the Algal Genetics group is supported by the CNRS, the ANR (project Bi-cycle ANR-10-BLAN-1727 and project Idealg ANR-10-BTBR-04-01), the Interreg program France (Channel)-England (project Marinexus), the UPMC and the European Research Council (grant agreement 638240). LM had a PhD grant (ARED) from the Region Bretagne.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Bachtrog D, 2014, PLOS BIOL, V12, DOI 10.1371/journal.pbio.1001899; Bachtrog D, 2011, TRENDS GENET, V27, P350, DOI 10.1016/j.tig.2011.05.005; Beukeboom L.W., EVOLUTION SEX DETERM; Bull JJ., 1983, EVOLUTION SEX DETERM; Charlesworth B, 2000, PHILOS T R SOC B, V355, P1563, DOI 10.1098/rstb.2000.0717; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2011, J EXP BOT, V62, P2425, DOI 10.1093/jxb/err117; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P262, DOI 10.1101/pdb.prot067942; Ferris P, 2010, SCIENCE, V328, P351, DOI 10.1126/science.1186222; Ferris PJ, 2002, GENETICS, V160, P181; Godfroy O, 2015, MAR GENOM, V24, P109, DOI 10.1016/j.margen.2015.03.007; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Herpin A, 2015, EMBO REP, V16, P1260, DOI 10.15252/embr.201540667; Jordan CY, 2012, EVOLUTION, V66, P505, DOI 10.1111/j.1558-5646.2011.01448.x; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Luthringer R, 2014, PERSPECT PHYCOL, V1, P11, DOI DOI 10.1127/2198-011X/2014/0002; Luthringer R, 2015, MOL BIOL EVOL, V32, P2973, DOI 10.1093/molbev/msv173; Presgraves DC, 2008, TRENDS GENET, V24, P336, DOI 10.1016/j.tig.2008.04.007; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Smeds L, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms6448; Tarver JE, 2015, NUCLEIC ACIDS RES, V43, P6384, DOI 10.1093/nar/gkv578; Togashi T, 2012, P NATL ACAD SCI USA, V109, P13692, DOI 10.1073/pnas.1203495109	24	4	4	0	31	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER	PARIS	23 RUE LINOIS, 75724 PARIS, FRANCE	1631-0691	1768-3238		CR BIOL	C. R. Biol.	JUL-AUG	2016	339	7-8					252	257		10.1016/j.crvi.2016.04.012			6	Biology	Life Sciences & Biomedicine - Other Topics	DR9WL	WOS:000380246700006	27236828	Other Gold, Green Published			2021-04-07	
J	Backorova, M; Maslanakova, I; Backor, M				Backorova, Miriam; Maslanakova, Ivana; Backor, Martin			Copper uptake and copper-induced physiological changes in the marine alga Cladophora prolifera (Roth.) Kutz. (Chlorophyta, Ulvophyceae)	BRAZILIAN JOURNAL OF BOTANY			English	Article						Algae; Metal uptake; Tolerance; Toxicity	MEMBRANE-LIPID PEROXIDATION; ECTOCARPUS-SILICULOSUS; HEAVY-METALS; LICHENS; STRESS; RESPONSES; TOXICITY; STRAINS; CU	Green algae of the genus Cladophora have been reported as being bioindicators of metal contamination all over the world. The degree of total as well as intracellular copper (Cu) uptakes by the alga after 24-h prolonged exposure with 0 (control), 50 and 500 mu M Cu doses, was compared with selected physiological markers. Increased intracellular Cu content in the Cladophora thalli was followed by decreased potassium (K) content, alteration of assimilation pigments composition, decreased integrity of chlorophyll a and its fluorescence. Increase of intracellular Cu was followed by the increases of TBARS and superoxide contents. While K content, chlorophyll a/b, chlorophyll a fluorescence and TBARS contents in C. prolifera were significantly altered as result of the lowest, 50 mu M Cu dose tested, levels of chlorophyll a + b and soluble proteins did not change even after application of the 500 mu M Cu dose.	[Backorova, Miriam] Univ Vet Med & Pharm, Dept Pharmacognosy & Bot, Komenskeho 73, Kosice 04181, Slovakia; [Maslanakova, Ivana; Backor, Martin] Safarik Univ, Dept Bot, Inst Biol & Ecol, Fac Sci, Manesova 23, Kosice 04167, Slovakia	Backorova, M (corresponding author), Univ Vet Med & Pharm, Dept Pharmacognosy & Bot, Komenskeho 73, Kosice 04181, Slovakia.	martin.backor@upjs.sk			Slovak Grant AgencyVedecka grantova agentura MSVVaS SR a SAV (VEGA) [VEGA 1/1238/12]	This work was financially supported by the Slovak Grant Agency (VEGA 1/1238/12). The authors offer their thanks to Kenneth Andrew Dvorsky M.Sc. M.Ed. (University of Toronto, Canada) for comments on the manuscript and Dr. Andrija Zelimir Lovric (Herbarium Adriaticum, Zagreb, Croatia) for help with algal identification.	Backor M, 2009, BIOL PLANTARUM, V53, P214, DOI 10.1007/s10535-009-0042-y; Backor M, 2007, BRYOLOGIST, V110, P100, DOI 10.1639/0007-2745(2007)110[100:UPCAML]2.0.CO;2; Backor M, 2009, PLANT GROWTH REGUL, V58, P279, DOI 10.1007/s10725-009-9376-x; Baumann HA, 2009, ECOTOX ENVIRON SAFE, V72, P1063, DOI 10.1016/j.ecoenv.2008.10.010; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; Chettri MK, 1998, ENVIRON EXP BOT, V39, P1, DOI 10.1016/S0098-8472(97)00024-5; Clark R. B., 2001, MARINE POLLUTION; Garty J, 2001, CRIT REV PLANT SCI, V20, P309, DOI 10.1016/S0735-2689(01)80040-X; Kalinowska R, 2010, ENVIRON POLLUT, V158, P2778, DOI 10.1016/j.envpol.2010.03.003; Kennish MJ, 1996, PRACTICAL HDB ESTUAR; Kovacik J, 2011, ECOTOXICOLOGY, V20, P348, DOI 10.1007/s10646-010-0585-x; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; Piovar J, 2011, PLANT GROWTH REGUL, V63, P81, DOI 10.1007/s10725-010-9515-4; Rocchetta I, 2009, NEW PHYTOL, V182, P405, DOI 10.1111/j.1469-8137.2009.02768.x; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; RONEN R, 1984, ENVIRON EXP BOT, V24, P239, DOI 10.1016/0098-8472(84)90004-2; Sabatini SE, 2011, PHYCOLOGIA, V50, P78, DOI 10.2216/09-101.1; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Schutzendubel A, 2002, J EXP BOT, V53, P1351, DOI 10.1093/jexbot/53.372.1351; STOHS SJ, 1995, FREE RADICAL BIO MED, V18, P321, DOI 10.1016/0891-5849(94)00159-H; Vavilin DV, 1998, J PHOTOCH PHOTOBIO B, V42, P233, DOI 10.1016/S1011-1344(98)00076-1; WELLBURN AR, 1994, J PLANT PHYSIOL, V144, P307, DOI 10.1016/S0176-1617(11)81192-2; Wissel H, 2008, ORG GEOCHEM, V39, P1545, DOI 10.1016/j.orggeochem.2008.07.014; Zulkifly SB, 2013, J PHYCOL, V49, P1, DOI 10.1111/jpy.12025	24	2	2	0	24	SOC BOTANICA SAO PAULO	SAO PAULO	CAIXA POSTAL 57088, SAO PAULO, SP 00000, BRAZIL	1806-9959			BRAZ J BOT	Braz. J. Bot.	JUN	2016	39	2					447	452		10.1007/s40415-016-0251-5			6	Plant Sciences	Plant Sciences	DQ1BC	WOS:000378934700006					2021-04-07	
J	Kinoshita, N; Shiba, K; Inaba, K; Fu, G; Nagasato, C; Motomura, T				Kinoshita, Nana; Shiba, Kogiku; Inaba, Kazuo; Fu, Gang; Nagasato, Chikako; Motomura, Taizo			Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						anterior flagellum; brown algae; chemotaxis; flagellar movement; gametes; posterior flagellum; thigomotaxis	SEX ATTRACTANT; MOVEMENT; SPERM; SPERMATOZOA; CA2+; CHEMOTAXIS; PHEROMONES; PHAEOPHYTA; RESPONSES; MOTILITY	Brown algae are members of the Stramenopiles and their gametes generally have two heterogeneous flagella: a long anterior flagellum (AF) with mastigonemes and a short posterior flagellum (PF). In this study, swimming paths and flagellar waveforms in free-swimming and thigmotactic-swimming male and female gametes and in male gametes during chemotaxis, were quantitatively analysed in the model brown alga Ectocarpus siliculosus. This analysis was performed using a high-speed video camera. It was revealed that the AF plays a role in changing the locomotion of male and female gametes from free-swimming to thigmotactic-swimming and also in changing the swimming path of male gametes from linear to circular during chemotaxis. In the presence of a sex pheromone, male gametes changed their swimming path from linear (swimming path curvature, 0-0.02 mu m(-1)) to middle and small circular path (swimming path curvature, 0.04-0.20 mu m(-1)). The flagellar asymmetry and the deflection angle of the AF became larger, whereas the oscillation pattern of the AF was stable. However, there was no correlation between the flagellar asymmetry and the deflection angle of the AF and the path curvature when the male gametes showed middle to small circular paths. The PF irregularly changed the deflection angle and the oscillation pattern was unstable depending on the gradient of the sex pheromone concentration. AF waveforms were independent of PF locomotion during chemotaxis. This means that the AF has the ability to change the swimming path of male gametes - for example, from a highly linear path to a circular path - while changes in locomotion from a middle circle path to a small circle path is the result of beating of the PF.	[Kinoshita, Nana] Hokkaido Univ, Grad Sch Environm Sci, Sapporo, Hokkaido 0600810, Japan; [Shiba, Kogiku; Inaba, Kazuo] Univ Tsukuba, Shimoda Marine Res Ctr, Shimoda, Shizuoka 4150025, Japan; [Fu, Gang; Nagasato, Chikako; Motomura, Taizo] Hokkaido Univ, Field Sci Ctr Northern Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510003, Japan	Motomura, T (corresponding author), Hokkaido Univ, Field Sci Ctr Northern Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510003, Japan.	motomura@fsc.hokudai.ac.jp			Japanese Association for Marine Biology [25-55, 26-56]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [16K07337, 26440160, 26840061] Funding Source: KAKEN	This study was partially supported by the Japanese Association for Marine Biology (25-55, 26-56).	Andersen RA, 2004, AM J BOT, V91, P1508, DOI 10.3732/ajb.91.10.1508; BABA SA, 1985, CELL MOTIL CYTOSKEL, V5, P475, DOI 10.1002/cm.970050605; BOUCK GB, 1969, J CELL BIOL, V40, P446, DOI 10.1083/jcb.40.2.446; CHWANG AT, 1971, PROC R SOC SER B-BIO, V178, P327, DOI 10.1098/rspb.1971.0068; Clayton M. N., 1989, SYSTEMATICS ASS SPEC, V38, P230; Fu G, 2014, PROTIST, V165, P662, DOI 10.1016/j.protis.2014.07.007; GELLER A, 1981, J EXP BIOL, V92, P53; GIBBONS IR, 1981, J CELL BIOL, V91, pS107, DOI 10.1083/jcb.91.3.107s; GOODENOUGH UW, 1983, J CELL BIOL, V96, P1610, DOI 10.1083/jcb.96.6.1610; HOLWILL MEJ, 1967, J EXP BIOL, V47, P267; Inaba K, 2003, ZOOL SCI, V20, P1043, DOI 10.2108/zsj.20.1043; Ishijima S, 2012, BIOL BULL-US, V222, P214, DOI 10.1086/BBLv222n3p214; JAHN TL, 1964, J PROTOZOOL, V11, P291, DOI 10.1111/j.1550-7408.1964.tb01756.x; Kaupp UB, 2008, ANNU REV PHYSIOL, V70, P93, DOI 10.1146/annurev.physiol.70.113006.100654; Kawai H, 2000, SYST ASSOC SPEC VOL, V59, P124; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KELLER JB, 1976, BIOPHYS J, V16, P151, DOI 10.1016/S0006-3495(76)85672-X; MAIER I, 1993, PLANT CELL ENVIRON, V16, P891, DOI 10.1111/j.1365-3040.1993.tb00513.x; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; Maier I, 1995, PROGR PHYCOL RES, V11, P51; Matsunaga S, 2010, PHOTOCHEM PHOTOBIOL, V86, P374, DOI 10.1111/j.1751-1097.2009.00676.x; MILLER RL, 1975, NATURE, V254, P244, DOI 10.1038/254244a0; MILLER RL, 1970, J EXP BIOL, V52, P699; MILLER RL, 1977, J EXP ZOOL, V202, P203, DOI 10.1002/jez.1402020209; Mitchell DR, 2000, J PHYCOL, V36, P261, DOI 10.1046/j.1529-8817.2000.99218.x; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1967, NATURWISSENSCHAFTEN, V54, P496; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; O' Kelly C. J., 1989, SYSTEMATICS ASS SPEC, V38, P256; Provasoli L, 1968, CULTURES COLLECTIONS, P63; Shiba K, 2006, ZYGOTE, V14, P23, DOI 10.1017/S0967199405003503; Shiba K, 2008, P NATL ACAD SCI USA, V105, P19312, DOI 10.1073/pnas.0808580105; Wood CD, 2005, J CELL BIOL, V169, P725, DOI 10.1083/jcb.200411001; Yoshida M, 2002, P NATL ACAD SCI USA, V99, P14831, DOI 10.1073/pnas.242470599; Yoshida M, 2011, MOL HUM REPROD, V17, P457, DOI 10.1093/molehr/gar041	37	12	12	0	15	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0967-0262	1469-4433		EUR J PHYCOL	Eur. J. Phycol.	MAY	2016	51	2					139	148		10.1080/09670262.2015.1109144			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	DI4VG	WOS:000373496900003		Green Accepted			2021-04-07	
J	Fu, G; Nagasato, C; Yamagishi, T; Kawai, H; Okuda, K; Takao, Y; Horiguchi, T; Motomura, T				Fu, Gang; Nagasato, Chikako; Yamagishi, Takahiro; Kawai, Hiroshi; Okuda, Kazuo; Takao, Yoshitake; Horiguchi, Takeo; Motomura, Taizo			Ubiquitous distribution of helmchrome in phototactic swarmers of the stramenopiles	PROTOPLASMA			English	Article						Brown algae; Flagella; Helmchrome; Photoreceptor; Phototaxis; Stramenopiles	ECTOCARPUS-SILICULOSUS ECTOCARPALES; BLUE-LIGHT RECEPTOR; FINE-STRUCTURE; FLAGELLAR AUTOFLUORESCENCE; PUTATIVE PHOTORECEPTOR; SCYTOSIPHON-LOMENTARIA; VOLTAGE DOMAINS; MALE GAMETE; BROWN ALGA; PHAEOPHYCEAE	Most swarmers (swimming cells) of the stramenopile group, ranging from unicellular protist to giant kelps (brown algae), have two heterogeneous flagella: a long anterior flagellum (AF) and a relatively shorter posterior flagellum (PF). These flagellated cells often exhibit phototaxis upon light stimulation, although the mechanism by which how the phototactic response is regulated remains largely unknown. A flavoprotein concentrating at the paraflagellar body (PFB) on the basal part of the PF, which can emit green autofluorescence under blue light irradiance, has been proposed as a possible blue light photoreceptor for brown algal phototaxis although the nature of the flavoprotein still remains elusive. Recently, we identified helmchrome as a PF-specific flavoprotein protein in a LC-MS/MS-based proteomics study of brown algal flagella (Fu et al. 2014). To verify the conservation of helmchrome, in the present study, the absence or presence and the localization of helmchrome in swarmers of various algal species were investigated. The results showed that helmchrome was only detected in phototactic swarmers but not the non-phototactic ones of the stramenopile group. Electron microscopy further revealed that the helmchrome detectable swarmers bear a conserved PFB-eyespot complex, which may serve as structural basis for light sensing. It is speculated that all three conserved properties: helmchrome, the PFB structure, and the eyespot apparatus, will be essential parts for phototaxis of stramenopile swarmers.	[Fu, Gang; Nagasato, Chikako; Motomura, Taizo] Hokkaido Univ, Field Sci Ctr No Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510013, Japan; [Yamagishi, Takahiro; Kawai, Hiroshi] Kobe Univ, Res Ctr Inland Seas, Nada Ku, Kobe, Hyogo 6578501, Japan; [Okuda, Kazuo] Kochi Univ, Grad Sch Integrated Arts & Sci, Kochi 7808520, Japan; [Takao, Yoshitake] Fukui Prefectural Univ, Fac Marine Biosci, Obama, Fukui 9170003, Japan; [Horiguchi, Takeo] Hokkaido Univ, Fac Sci, Sapporo, Hokkaido 0600810, Japan	Motomura, T (corresponding author), Hokkaido Univ, Field Sci Ctr No Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510013, Japan.	motomura@fsc.hokudai.ac.jp	Horiguchi, Takeo/D-7612-2012	Horiguchi, Takeo/0000-0002-6118-8460	Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [25840128] Funding Source: KAKEN		AMON JP, 1968, J PROTOZOOL, V15, P543, DOI 10.1111/j.1550-7408.1968.tb02172.x; Amon JP, 2004, MYCOLOGIA, V96, P463, DOI 10.2307/3762166; Avelar GM, 2014, CURR BIOL, V24, P1234, DOI 10.1016/j.cub.2014.04.009; Ballario P, 1996, EMBO J, V15, P1650, DOI 10.1002/j.1460-2075.1996.tb00510.x; Barsanti L, 1997, BIOPHYS J, V72, P545, DOI 10.1016/S0006-3495(97)78694-6; Berthold P, 2008, PLANT CELL, V20, P1665, DOI 10.1105/tpc.108.057919; Brunelle SA, 2007, J PHYCOL, V43, P509, DOI 10.1111/j.1529-8817.2007.00339.x; Cheng P, 2003, P NATL ACAD SCI USA, V100, P5938, DOI 10.1073/pnas.1031791100; Cheng P, 2003, J BIOL CHEM, V278, P3801, DOI 10.1074/jbc.M209592200; Cho HY, 2007, PLANT PHYSIOL, V143, P517, DOI 10.1104/pp.106.089839; Christie JM, 2007, ANNU REV PLANT BIOL, V58, P21, DOI 10.1146/annurev.arplant.58.032806.103951; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; COLEMAN AW, 1988, J PHYCOL, V24, P118; Crosson S, 2001, P NATL ACAD SCI USA, V98, P2995, DOI 10.1073/pnas.051520298; Crosson S, 2003, BIOCHEMISTRY-US, V42, P2, DOI 10.1021/bi026978l; Crosthwaite SK, 1997, SCIENCE, V276, P763, DOI 10.1126/science.276.5313.763; Deng YY, 2014, MAR BIOTECHNOL, V16, P135, DOI 10.1007/s10126-013-9539-7; DODGE JD, 1983, BIOSYSTEMS, V16, P259, DOI 10.1016/0303-2647(83)90009-6; Flores-Moya A, 2002, J PHOTOCH PHOTOBIO B, V66, P134, DOI 10.1016/S1011-1344(02)00233-6; FOSTER KW, 1984, NATURE, V311, P756, DOI 10.1038/311756a0; Fu G, 2014, PROTIST, V165, P662, DOI 10.1016/j.protis.2014.07.007; Fu G, 2013, PROTOPLASMA, V250, P261, DOI 10.1007/s00709-012-0405-7; Hegemann P, 2008, ANNU REV PLANT BIOL, V59, P167, DOI 10.1146/annurev.arplant.59.032607.092847; Heintzen C, 2001, CELL, V104, P453, DOI 10.1016/S0092-8674(01)00232-X; Horiguchi Takeo, 1999, Phycological Research, V47, P101, DOI 10.1111/j.1440-1835.1999.tb00290.x; Huala E, 1997, SCIENCE, V278, P2120, DOI 10.1126/science.278.5346.2120; Huysman MJJ, 2013, PLANT CELL, V25, P215, DOI 10.1105/tpc.112.106377; HYAMS JS, 1982, J CELL SCI, V55, P199; Idei M, 2013, PROTOPLASMA, V250, P833, DOI 10.1007/s00709-012-0465-8; Iseki M, 2002, NATURE, V415, P1047, DOI 10.1038/4151047a; Ishikawa M, 2009, PLANTA, V230, P543, DOI 10.1007/s00425-009-0967-6; Ito S, 2012, MOL PLANT, V5, P573, DOI 10.1093/mp/sss013; Kami C, 2010, CURR TOP DEV BIOL, V91, P29, DOI 10.1016/S0070-2153(10)91002-8; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; Kawai H, 2003, PROTIST, V154, P211, DOI 10.1078/143446103322166518; KAWAI H, 1991, PROTOPLASMA, V161, P17, DOI 10.1007/BF01328893; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KAWAI H, 1988, J PHYCOL, V24, P114; KAWAI H, 1992, BOT MAG TOKYO, V105, P171, DOI 10.1007/BF02489413; Kianianmomeni A, 2014, PLANTA, V239, P1, DOI 10.1007/s00425-013-1962-5; KIVIC PA, 1972, PLANTA, V105, P1, DOI 10.1007/BF00385158; Krauss U, 2009, J BACTERIOL, V191, P7234, DOI 10.1128/JB.00923-09; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; Lebert M, 2001, PHOTOMOVEMENT, V1, P297; LIU SM, 1990, FEMS MICROBIOL ECOL, V73, P91, DOI 10.1016/0378-1097(90)90655-A; LIU SM, 1994, PHOTOCHEM PHOTOBIOL, V59, P86, DOI 10.1111/j.1751-1097.1994.tb05005.x; Lokhorst GM, 2003, PHYCOLOGIA, V42, P31, DOI 10.2216/i0031-8884-42-1-31.1; Losi A, 2012, ANNU REV PLANT BIOL, V63, P49, DOI 10.1146/annurev-arplant-042811-105538; Maier I, 1997, EUR J PHYCOL, V32, P255; Maier I, 1997, EUR J PHYCOL, V32, P241; Matsunaga S, 2010, PHOTOCHEM PHOTOBIOL, V86, P374, DOI 10.1111/j.1751-1097.2009.00676.x; Mistry J, 2013, NUCLEIC ACIDS RES, V41, DOI [10.1093/nar/gkt263, 10.1093/nar/gkt1223]; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MOTOMURA T, 1981, B JPN SOC SCI FISH, V47, P1535; MOTOMURA T, 1994, PROTOPLASMA, V178, P97, DOI 10.1007/BF01545960; MULLER DG, 1979, NATURE, V279, P430, DOI 10.1038/279430a0; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; Nagasato C, 2001, PHYCOLOGIA, V40, P411, DOI 10.2216/i0031-8884-40-5-411.1; Nagasato C, 2002, PROTOPLASMA, V219, P140, DOI 10.1007/s007090200015; Nagel G, 2002, SCIENCE, V296, P2395, DOI 10.1126/science.1072068; Nagel G, 2003, P NATL ACAD SCI USA, V100, P13940, DOI 10.1073/pnas.1936192100; Nozue K, 1998, P NATL ACAD SCI USA, V95, P15826, DOI 10.1073/pnas.95.26.15826; OSBORN M, 1982, METHOD CELL BIOL, V24, P97; PECORA R A, 1973, British Phycological Journal, V8, P321; Perveen Z, 2006, BIOTECHNOL LETT, V28, P197, DOI 10.1007/s10529-005-5335-4; Provasoli L, 1968, CULTURES COLLECTIONS, P63; Reynolds ES, 1963, J CELL BIOL, V3, P813; Saranak J, 1997, NATURE, V387, P465, DOI 10.1038/387465a0; Sineshchekov OA, 2002, P NATL ACAD SCI USA, V99, P8689, DOI 10.1073/pnas.122243399; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; Suetsugu N, 2005, P NATL ACAD SCI USA, V102, P13705, DOI 10.1073/pnas.0504734102; Suzuki T, 2003, BIOCHEM BIOPH RES CO, V301, P711, DOI 10.1016/S0006-291X(02)03079-6; Takahashi F, 2007, P NATL ACAD SCI USA, V104, P19625, DOI 10.1073/pnas.0707692104; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Walker Claire A., 2007, Fungal Biology Reviews, V21, P10, DOI 10.1016/j.fbr.2007.02.001	76	5	6	0	15	SPRINGER WIEN	WIEN	SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA	0033-183X	1615-6102		PROTOPLASMA	Protoplasma	MAY	2016	253	3			SI		929	941		10.1007/s00709-015-0857-7			13	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	DI5ZH	WOS:000373578500027	26202473				2021-04-07	
J	Liu, F; Pang, S				Liu, Feng; Pang, Shaojun			Complete mitochondrial genome of the brown alga Scytosiphon lomentaria ( Scytosiphonaceae, Phaeophyceae)	MITOCHONDRIAL DNA PART A			English	Article						Brown alga; mitochondrial genome; Phaeophyceae; Scytosiphonaceae; Scytosiphon lomentaria		We determined the complete mitochondrial genome of Scytosiphon lomentaria (Lyngbye) Link, which is the first representative of the genus Scytosiphon C. Agardh. The circular mitogenome of S. lomentaria is 36,918bp in length, with the overall A+T content of 65.86%. The genome contains 67 genes, including 3 ribosomal RNA genes (rRNA), 25 transfer RNA genes (tRNA), 35 protein-coding genes and 4 unidentified open reading frames (ORFs). The gene order of S. lomentaria mitogenome conforms to that of Ectocarpales mitogenomes (not including Pylaiella littoralis), i.e. Petalonia fascia, and Ectocarpus siliculosus, but differs from Laminariales, Desmarestiales, Fucales and Dictyotales with position variation of several genes. The S. lomentaria mitogenome has an overall nucleotide sequence identity of 80.4% with P. fascia, and 74.9% with E. siliculosus. The present data is of value to phylogenetic analyses of such a diverse Scytosiphonaceae family as well as to understanding of mitogenome evolution in brown algae.	[Liu, Feng; Pang, Shaojun] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China	Liu, F (corresponding author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.	liufeng@qdio.ac.cn; sjpang@qdio.ac.cn			National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41206146]; Scientific Research Foundation for Outstanding Young Scientists of Shandong Province [BS2013HZ004]; Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture [K201311]; Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, State Oceanic Administration [MATHAB201408]	This work was supported by the National Natural Science Foundation of China (No. 41206146), the Scientific Research Foundation for Outstanding Young Scientists of Shandong Province (No. BS2013HZ004), the Open Reseach Fund of Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture (No. K201311), and the Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, State Oceanic Administration (No. MATHAB201408). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.	Cho Ga Youn, 2006, Algae, V21, P175; Kogame K, 2005, EUR J PHYCOL, V40, P313, DOI 10.1080/09670260500193008; Liu F, 2016, MITOCHONDRIAL DNA A, V27, P1158, DOI 10.3109/19401736.2014.936417; Liu F, 2015, J APPL PHYCOL, V27, P1021, DOI 10.1007/s10811-014-0386-3; Liu F, 2015, J APPL PHYCOL, V27, P469, DOI 10.1007/s10811-014-0295-5; Nagasato C, 2004, CELL MOTIL CYTOSKEL, V59, P109, DOI 10.1002/cm.20021; Nagasato C, 2002, J CELL SCI, V115, P2541; Secq MPO, 2006, CURR GENET, V49, P47, DOI 10.1007/s00294-005-0031-4; Tseng C.K., 2009, SEAWEEDS YELLOW SEA; [邢永泽 XING YongZe], 2011, [海洋与湖沼, Oceanologia et Limnologia Sinica], V42, P101; [张文健 Zhang Wenjian], 2013, [海洋与湖沼, Oceanologia et Limnologia Sinica], V44, P1661	11	2	2	0	9	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	2470-1394	2470-1408		MITOCHONDRIAL DNA A	Mitochondrial DNA Part A	MAR 3	2016	27	2					1494	1496		10.3109/19401736.2014.953108			3	Genetics & Heredity	Genetics & Heredity	CY5RH	WOS:000366464400293	25186060				2021-04-07	
J	Herve, C; Simeon, A; Jam, M; Cassin, A; Johnson, KL; Salmean, AA; Willats, WGT; Doblin, MS; Bacic, A; Kloareg, B				Herve, Cecile; Simeon, Amandine; Jam, Murielle; Cassin, Andrew; Johnson, Kim L.; Salmean, Armando A.; Willats, William G. T.; Doblin, Monika S.; Bacic, Antony; Kloareg, Bernard			Arabinogalactan proteins have deep roots in eukaryotes: identification of genes and epitopes in brown algae and their role in Fucus serratus embryo development	NEW PHYTOLOGIST			English	Article						arabinogalactan proteins (AGPs); brown algae; cell elongation; cell polarity; cell wall; cell wall evolution; Ectocarpus; Fucus embryo	GLUCOSYL YARIV REAGENT; PLANT-CELL WALLS; EXTRACELLULAR-MATRIX; POLLEN-TUBE; ARABIDOPSIS; EVOLUTION; FAMILY; INSIGHTS; ZYGOTES; GROWTH	Arabinogalactan proteins (AGPs) are highly glycosylated, hydroxyproline-rich proteins found at the cell surface of plants, where they play key roles in developmental processes. Brown algae are marine, multicellular, photosynthetic eukaryotes. They belong to the phylum Stramenopiles, which is unrelated to land plants and green algae (Chloroplastida). Brown algae share common evolutionary features with other multicellular organisms, including a carbohydrate-rich cell wall. They differ markedly from plants in their cell wall composition, and AGPs have not been reported in brown algae. Here we investigated the presence of chimeric AGP-like core proteins in this lineage. We report that the genome sequence of the brown algal model Ectocarpus siliculosus encodes AGP protein backbone motifs, in a gene context that differs considerably from what is known in land plants. We showed the occurrence of AGP glycan epitopes in a range of brown algal cell wall extracts. We demonstrated that these chimeric AGP-like core proteins are developmentally regulated in embryos of the order Fucales and showed that AGP loss of function seriously impairs the course of early embryogenesis. Our findings shine a new light on the role of AGPs in cell wall sensing and raise questions about the origin and evolution of AGPs in eukaryotes.	[Herve, Cecile; Simeon, Amandine; Jam, Murielle; Kloareg, Bernard] Univ Paris 06, Sorbonne Univ, UMR 8227, Stn Biol Roscoff,Integrat Biol Marine Models, CS 90074, F-29688 Roscoff, France; [Herve, Cecile; Simeon, Amandine; Jam, Murielle; Kloareg, Bernard] CNRS, UMR 8227, Integrat Biol Marine Models, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; [Cassin, Andrew; Johnson, Kim L.; Doblin, Monika S.; Bacic, Antony] Univ Melbourne, Sch BioSci, ARC Ctr Excellence Plant Cell Walls, Melbourne, Vic, Australia; [Salmean, Armando A.; Willats, William G. T.] Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, Copenhagen, Denmark	Herve, C (corresponding author), Univ Paris 06, Sorbonne Univ, UMR 8227, Stn Biol Roscoff,Integrat Biol Marine Models, CS 90074, F-29688 Roscoff, France.; Herve, C (corresponding author), CNRS, UMR 8227, Integrat Biol Marine Models, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.	cecile.herve@sb-roscoff.fr		Herve, Cecile/0000-0001-6649-8137; Bacic, Tony/0000-0001-7483-8605; Johnson, Kim/0000-0001-6917-7742	Australia Research CouncilAustralian Research Council [CE10001007]; Brittany regionRegion Bretagne [ARED_8979 ECTOPAR]	K.L.J., M.S.D. and A.B. acknowledge the support of a grant from the Australia Research Council to the ARC Centre of Excellence in Plant Cell Walls (CE10001007). A.S. received a grant from the Brittany region (ARED_8979 ECTOPAR).	BERGER F, 1994, SCIENCE, V263, P1421, DOI 10.1126/science.263.5152.1421; Bisgrove Sherryl R., 2008, V9, P323, DOI 10.1007/7089_2007_134; Bisgrove SR, 2001, PLANTA, V212, P648, DOI 10.1007/s004250000434; Borderies G, 2004, EUR J CELL BIOL, V83, P205, DOI 10.1078/0171-9335-00378; BROWNLEE C, 1995, TRENDS GENET, V11, P344, DOI 10.1016/S0168-9525(00)89104-0; Cantarel BL, 2009, NUCLEIC ACIDS RES, V37, pD233, DOI 10.1093/nar/gkn663; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Doblin MS, 2010, FUNCT PLANT BIOL, V37, P357, DOI 10.1071/FP09279; Domozych DS, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00082; Dorrell RG, 2011, EUKARYOT CELL, V10, P856, DOI 10.1128/EC.00326-10; Dupres V, 2009, NAT CHEM BIOL, V5, P857, DOI 10.1038/nchembio.220; Eisenhaber B, 2003, PLANT PHYSIOL, V133, P1691, DOI 10.1104/pp.103.023580; Ellis M, 2010, PLANT PHYSIOL, V153, P403, DOI 10.1104/pp.110.156000; Estevez JM, 2009, GLYCOBIOLOGY, V19, P212, DOI 10.1093/glycob/cwn101; Geshi N, 2013, PLANT J, V76, P128, DOI 10.1111/tpj.12281; Gish LA, 2011, PLANT J, V66, P117, DOI 10.1111/j.1365-313X.2011.04518.x; Gorres KL, 2010, CRIT REV BIOCHEM MOL, V45, P106, DOI 10.3109/10409231003627991; GOTELLI IB, 1968, AM J BOT, V55, P907, DOI 10.2307/2440555; Gualtieri T, 2004, YEAST, V21, P1107, DOI 10.1002/yea.1155; Huang GQ, 2013, PLANT PHYSIOL, V161, P1278, DOI 10.1104/pp.112.203760; Ischebeck T, 2010, PROTOPLASMA, V240, P13, DOI 10.1007/s00709-009-0093-0; Jackson OD, 2010, THESIS; Jackson O, 2012, MOL PLANT MICROBE IN, V25, P1338, DOI 10.1094/MPMI-04-12-0095-R; Jauh GY, 1996, PLANTA, V199, P251; Johnson KL, 2003, PLANT PHYSIOL, V133, P1911, DOI 10.1104/pp.103.031237; KREUGER M, 1993, PLANTA, V189, P243, DOI 10.1007/BF00195083; KROPF DL, 1988, SCIENCE, V239, P187, DOI 10.1126/science.3336780; Lee KJD, 2005, PLANT CELL, V17, P3051, DOI 10.1105/tpc.105.034413; McCabe PF, 1997, PLANT CELL, V9, P2225, DOI 10.1105/tpc.9.12.2225; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Moller I, 2007, PLANT J, V50, P1118, DOI 10.1111/j.1365-313X.2007.03114.x; Mollet JC, 2002, PROTOPLASMA, V219, P89, DOI 10.1007/s007090200009; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; Naumoff DG, 2011, MOL BIOL+, V45, P647, DOI 10.1134/S0026893311030113; Nguema-Ona E, 2012, ANN BOT-LONDON, V110, P383, DOI 10.1093/aob/mcs143; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; O'Keeffe AH, 2005, MOL BIOCHEM PARASIT, V140, P61, DOI 10.1016/j.molbiopara.2004.12.003; PERKINS SJ, 1994, J MOL BIOL, V238, P104, DOI 10.1006/jmbi.1994.1271; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Qin Y, 2006, J EXP BOT, V57, P2061, DOI 10.1093/jxb/erj159; Qu YM, 2008, PLANT MOL BIOL, V68, P43, DOI 10.1007/s11103-008-9351-3; QUATRANO RS, 1976, PLANT PHYSIOL, V58, P224, DOI 10.1104/pp.58.2.224; QUATRANO RS, 1991, DEVELOPMENT, P11; SCHINDLER T, 1995, PLANT J, V7, P25, DOI 10.1046/j.1365-313X.1995.07010025.x; Schultz CJ, 2002, PLANT PHYSIOL, V129, P1448, DOI 10.1104/pp.003459; Showalter AM, 2010, PLANT PHYSIOL, V153, P485, DOI 10.1104/pp.110.156554; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Tan L, 2013, PLANT CELL, V25, P270, DOI 10.1105/tpc.112.107334; Tan L, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00140; Tang XC, 2006, J EXP BOT, V57, P2639, DOI 10.1093/jxb/erl027; Thompson HJM, 1998, PLANTA, V205, P32, DOI 10.1007/s004250050293; Torode TA, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0118366; van Hengel AJ, 2001, PLANT PHYSIOL, V125, P1880, DOI 10.1104/pp.125.4.1880; Verna J, 1997, P NATL ACAD SCI USA, V94, P13804, DOI 10.1073/pnas.94.25.13804; Vissenberg K, 2001, J EXP BOT, V52, P2161, DOI 10.1093/jexbot/52.364.2161; WAGNER VT, 1992, P NATL ACAD SCI USA, V89, P3644, DOI 10.1073/pnas.89.8.3644; Willats WGT, 1996, PLANT J, V9, P919, DOI 10.1046/j.1365-313X.1996.9060919.x; Wolf S, 2012, ANNU REV PLANT BIOL, V63, P381, DOI 10.1146/annurev-arplant-042811-105449; Wu YY, 2010, J BIOL CHEM, V285, P13638, DOI 10.1074/jbc.M110.102715; Yu M, 2012, BMC PLANT BIOL, V12, DOI 10.1186/1471-2229-12-126; Zhang Y, 2003, PLANT MOL BIOL, V52, P91, DOI 10.1023/A:1023978210001	64	32	33	2	38	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X	1469-8137		NEW PHYTOL	New Phytol.	MAR	2016	209	4					1428	1441		10.1111/nph.13786			14	Plant Sciences	Plant Sciences	DI3DY	WOS:000373379800015	26667994				2021-04-07	
J	Tong, SM; Xi, HX; Ai, KJ; Hou, HS				Tong, Shaoming; Xi, Haixiu; Ai, Kejun; Hou, Hesheng			Cloning and expression analysis of UpPDS gene in Ulva pertusa	GENES & GENOMICS			English	Article						Phytoene; Desaturase; Phylogenetic; Ulva pertusa; Sequence analysis; Expression	ENCODING PHYTOENE DESATURASE; CAROTENOID BIOSYNTHESIS; IN-VITRO; ACCUMULATION; ASTAXANTHIN; CHLOROPLAST; PATHWAY	Phytoene desaturase (PDS) is an essential constituent of several carotenoid biosynthetic enzymes, which catalyzes phytoene into zeta-carotenoids. Here, a cDNA encoding PDS gene named UpPDS in Ulva pertusa was cloned by the approaches of expressed sequence tag sequencing of suppression subtractive hybridization cDNA library and rapid amplification of cDNA ends technique. The results showed that the full-length cDNA of UpPDS was 2029 bp, containing 1599 bp open reading frame and encoding 532 amino acids. Homology analysis showed that the deduced UpPDS protein was highly homologous to the PDS proteins from some algal species. Phylogenetic analysis indicated that the UpPDS was clustered together with PDS of Ectocarpus siliculosus firstly and was more close to PDS proteins from Chlamydomonas reinhardtii and Volvox carteri. The RT-qPCR analysis indicated that the transcript level of UpPDS was upregulated during the period of emersion.	[Tong, Shaoming; Xi, Haixiu; Ai, Kejun; Hou, Hesheng] Liaoning Normal Univ, Coll Life Sci, Dalian 116081, Peoples R China; [Tong, Shaoming; Xi, Haixiu; Ai, Kejun; Hou, Hesheng] Key Lab Plant Biotechnol Liaoning Prov, Dalian 116081, Peoples R China	Hou, HS (corresponding author), Liaoning Normal Univ, Coll Life Sci, Dalian 116081, Peoples R China.; Hou, HS (corresponding author), Key Lab Plant Biotechnol Liaoning Prov, Dalian 116081, Peoples R China.	hesheng_hou@126.com					Arnold K, 2006, BIOINFORMATICS, V22, P195, DOI 10.1093/bioinformatics/bti770; Botella-Pavia P, 2004, PLANT J, V40, P188, DOI 10.1111/j.1365-313X.2004.02198.x; Chang ZY, 1996, J BIOL CHEM, V271, P7218, DOI 10.1074/jbc.271.12.7218; Cong L, 2010, MOL BIOL REP, V37, P3351, DOI 10.1007/s11033-009-9922-7; Del Campo JA, 2004, APPL MICROBIOL BIOT, V64, P848, DOI 10.1007/s00253-003-1510-5; DEMMIGADAMS B, 1993, PLANT PHYSIOL, V103, P1413, DOI 10.1104/pp.103.4.1413; Emanuelsson O, 1999, PROTEIN SCI, V8, P978, DOI 10.1110/ps.8.5.978; Emanuelsson O, 2007, NAT PROTOC, V2, P953, DOI 10.1038/nprot.2007.131; Grunewald K, 2000, PLANT PHYSIOL, V122, P1261, DOI 10.1104/pp.122.4.1261; Hirschberg J, 2001, CURR OPIN PLANT BIOL, V4, P210, DOI 10.1016/S1369-5266(00)00163-1; Huang JC, 2008, J PHYCOL, V44, P684, DOI 10.1111/j.1529-8817.2008.00511.x; Kita M, 2001, BIOSCI BIOTECH BIOCH, V65, P1424, DOI 10.1271/bbb.65.1424; [李世国 LI Shiguo], 2011, [海洋渔业, Marine Fisheries], V33, P172; Li ZH, 1996, PLANT MOL BIOL, V30, P269, DOI 10.1007/BF00020113; MANN V, 1994, PLANT MOL BIOL, V24, P429, DOI 10.1007/BF00024111; Qi HM, 2006, BIOORG MED CHEM LETT, V16, P2441, DOI 10.1016/j.bmcl.2006.01.076; Rowlands MG, 2004, ANAL BIOCHEM, V327, P176, DOI 10.1016/j.ab.2003.10.038; Ruyter-Spira C, 2013, TRENDS PLANT SCI, V18, P72, DOI 10.1016/j.tplants.2012.10.003; SANDMANN G, 1994, EUR J BIOCHEM, V223, P7, DOI 10.1111/j.1432-1033.1994.tb18961.x; Scotto-Lavino E, 2006, NAT PROTOC, V1, P2742, DOI 10.1038/nprot.2006.481; Seo M, 2002, TRENDS PLANT SCI, V7, P41, DOI 10.1016/S1360-1385(01)02187-2; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tran PT, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0042196; Van Dien SJ, 2003, APPL ENVIRON MICROB, V69, P7563, DOI 10.1128/AEM.69.12.7563-7566.2003; Yan P, 2011, MOL BIOL REP, V38, P785, DOI 10.1007/s11033-010-0167-2; Zhao DQ, 2011, MOL BIOL REP, V38, P3935, DOI 10.1007/s11033-010-0510-7; Zhu YH, 2005, J AGR FOOD CHEM, V53, P5593, DOI 10.1021/jf0506838	27	1	3	1	5	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	1976-9571	2092-9293		GENES GENOM	Genes Genom.	MAR	2016	38	3					285	292		10.1007/s13258-015-0365-3			8	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity	Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity	DF3MJ	WOS:000371248000005					2021-04-07	
J	Pandey, RS; Azad, RK				Pandey, Ravi S.; Azad, Rajeev K.			Deciphering evolutionary strata on plant sex chromosomes and fungal mating-type chromosomes through compositional segmentation	PLANT MOLECULAR BIOLOGY			English	Article						Evolutionary strata; Plant sex chromosomes; Algal sex chromosomes; Fungal mating-type chromosomes; Sex chromosome evolution; DNA segmentation	HUMAN X-CHROMOSOME; ANTHER-SMUT FUNGUS; Y-CHROMOSOME; SILENE-LATIFOLIA; MICROBOTRYUM-VIOLACEUM; DNA-SEQUENCES; RECOMBINATION; DEGENERATION; POPULUS; REGION	Sex chromosomes have evolved from a pair of homologous autosomes which differentiated into sex determination systems, such as XY or ZW system, as a consequence of successive recombination suppression between the gametologous chromosomes. Identifying the regions of recombination suppression, namely, the "evolutionary strata", is central to understanding the history and dynamics of sex chromosome evolution. Evolution of sex chromosomes as a consequence of serial recombination suppressions is well-studied for mammals and birds, but not for plants, although 48 dioecious plants have already been reported. Only two plants Silene latifolia and papaya have been studied until now for the presence of evolutionary strata on their X chromosomes, made possible by the sequencing of sex-linked genes on both the X and Y chromosomes, which is a requirement of all current methods that determine stratum structure based on the comparison of gametologous sex chromosomes. To circumvent this limitation and detect strata even if only the sequence of sex chromosome in the homogametic sex (i.e. X or Z chromosome) is available, we have developed an integrated segmentation and clustering method. In application to gene sequences on the papaya X chromosome and protein-coding sequences on the S. latifolia X chromosome, our method could decipher all known evolutionary strata, as reported by previous studies. Our method, after validating on known strata on the papaya and S. latifolia X chromosome, was applied to the chromosome 19 of Populus trichocarpa, an incipient sex chromosome, deciphering two, yet unknown, evolutionary strata. In addition, we applied this approach to the recently sequenced sex chromosome V of the brown alga Ectocarpus sp. that has a haploid sex determination system (UV system) recovering the sex determining and pseudoautosomal regions, and then to the mating-type chromosomes of an anther-smut fungus Microbotryum lychnidis-dioicae predicting five strata in the non-recombining region of both the chromosomes.	[Pandey, Ravi S.; Azad, Rajeev K.] Univ N Texas, Dept Biol Sci, Denton, TX 76203 USA; [Azad, Rajeev K.] Univ N Texas, Dept Math, Denton, TX 76203 USA	Azad, RK (corresponding author), Univ N Texas, Dept Biol Sci, Denton, TX 76203 USA.; Azad, RK (corresponding author), Univ N Texas, Dept Math, Denton, TX 76203 USA.	Rajeev.Azad@unt.edu	Pandey, Ravi/J-9611-2019	Pandey, Ravi/0000-0001-9567-2851	faculty start-up fund; JFSRF award from the University of North Texas; Beth Baird graduate student scholarship	This work was supported by a faculty start-up fund and JFSRF award from the University of North Texas to RKA and a Beth Baird graduate student scholarship to RSP.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Arvey AJ, 2009, NUCLEIC ACIDS RES, V37, P5255, DOI 10.1093/nar/gkp576; Azad RK, 2013, NUCLEIC ACIDS RES, V41, DOI 10.1093/nar/gks917; Azad RK, 2002, PHYS REV E, V65, DOI 10.1103/PhysRevE.65.051909; Bachtrog D, 2008, GENETICS, V179, P1513, DOI 10.1534/genetics.107.084012; Bachtrog D, 2013, NAT REV GENET, V14, P113, DOI 10.1038/nrg3366; Badouin H, 2015, GENETICS, V200, P1275, DOI 10.1534/genetics.115.177709; Bergero R, 2007, GENETICS, V175, P1945, DOI 10.1534/genetics.106.070110; Bergero R, 2013, GENETICS, V194, P673, DOI 10.1534/genetics.113.150755; Bergero R, 2011, CURR BIOL, V21, P1470, DOI 10.1016/j.cub.2011.07.032; BernaolaGalvan P, 1996, PHYS REV E, V53, P5181, DOI 10.1103/PhysRevE.53.5181; BROOKFIELD JFY, 1991, GENETICS, V128, P471; CHARLESWORTH B, 1978, P NATL ACAD SCI USA, V75, P5618, DOI 10.1073/pnas.75.11.5618; Charlesworth B, 2000, PHILOS T R SOC B, V355, P1563, DOI 10.1098/rstb.2000.0717; Charlesworth D, 2005, HEREDITY, V95, P118, DOI 10.1038/sj.hdy.6800697; Dolgin ES, 2008, GENETICS, V178, P2169, DOI 10.1534/genetics.107.082743; Filatov DA, 2005, GENETICS, V170, P975, DOI 10.1534/genetics.104.037069; Finnegan DJ, 1992, GENOME DROSOPHILA ME, P1096; Fontanillas E, 2015, MOL BIOL EVOL, V32, P928, DOI 10.1093/molbev/msu396; Gaudet M, 2008, TREE GENET GENOMES, V4, P25, DOI 10.1007/s11295-007-0085-1; Geraldes A, 2015, MOL ECOL, V24, P3243, DOI 10.1111/mec.13126; Grosse I, 2002, PHYS REV E, V65, DOI 10.1103/PhysRevE.65.041905; Hobza R, 2007, MOL GENET GENOMICS, V278, P633, DOI 10.1007/s00438-007-0279-0; Hood ME, 2004, GENETICS, V168, P141, DOI 10.1534/genetics.104.029900; Hood ME, 2013, GENETICS, V193, P309, DOI 10.1534/genetics.112.146266; Hou J, 2015, SCI REP-UK, V5, DOI 10.1038/srep09076; Jordan CY, 2012, EVOLUTION, V66, P505, DOI 10.1111/j.1558-5646.2011.01448.x; Kirkpatrick M, 2006, GENETICS, V173, P419, DOI 10.1534/genetics.105.047985; Lahn BT, 1999, SCIENCE, V286, P964, DOI 10.1126/science.286.5441.964; Lemaitre C, 2009, GENOME BIOL EVOL, V1, P56, DOI 10.1093/gbe/evp006; Lemaitre JF, 2009, J EVOLUTION BIOL, V22, P2215, DOI 10.1111/j.1420-9101.2009.01837.x; Li WT, 2001, PHYS REV LETT, V86, P5815, DOI 10.1103/PhysRevLett.86.5815; LIN JH, 1991, IEEE T INFORM THEORY, V37, P145, DOI 10.1109/18.61115; Liu ZY, 2004, NATURE, V427, P348, DOI 10.1038/nature02228; Ming R, 2007, CURR OPIN PLANT BIOL, V10, P123, DOI 10.1016/j.pbi.2007.01.013; Ming R, 2011, ANNU REV PLANT BIOL, V62, P485, DOI 10.1146/annurev-arplant-042110-103914; MONTGOMERY E, 1987, GENET RES, V49, P31, DOI 10.1017/S0016672300026707; MULLER HJ, 1964, MUTAT RES, V1, P2, DOI 10.1016/0027-5107(64)90047-8; Nam K, 2008, GENETICS, V180, P1131, DOI 10.1534/genetics.108.090324; NEI M, 1970, AM NAT, V104, P311, DOI 10.1086/282665; Pandey RS, 2013, GENOME BIOL EVOL, V5, P1863, DOI 10.1093/gbe/evt139; RICE WR, 1984, EVOLUTION, V38, P735, DOI [10.2307/2408385, 10.1111/j.1558-5646.1984.tb00346.x]; RICE WR, 1987, EVOLUTION, V41, P911, DOI 10.1111/j.1558-5646.1987.tb05864.x; Rice WR, 1996, BIOSCIENCE, V46, P331, DOI 10.2307/1312947; Ross MT, 2005, NATURE, V434, P325, DOI 10.1038/nature03440; Sandstedt SA, 2004, GENOME RES, V14, P267, DOI 10.1101/gr.1796204; Sayres MAW, 2013, MOL BIOL EVOL, V30, P781, DOI 10.1093/molbev/mss267; Skaletsky H, 2003, NATURE, V423, P825, DOI 10.1038/nature01722; Smit AFA, 2013, REPEATMAKSER OPEN 4; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Thakur V, 2007, PHYS REV E, V75, DOI 10.1103/PhysRevE.75.011915; Tuskan GA, 2012, TREE GENET GENOMES, V8, P559, DOI 10.1007/s11295-012-0495-6; Veyrunes F, 2008, GENOME RES, V18, P965, DOI 10.1101/gr.7101908; Votintseva AA, 2009, GENETICS, V182, P1391, DOI 10.1534/genetics.109.103192; Wang JP, 2012, P NATL ACAD SCI USA, V109, P13710, DOI 10.1073/pnas.1207833109; Whittle CA, 2015, GENETICS, V199, P809, DOI 10.1534/genetics.114.171702; Wilson MA, 2009, PLOS GENET, V5, DOI 10.1371/journal.pgen.1000568; Yin T, 2008, GENOME RES, V18, P422, DOI 10.1101/gr.7076308; Yu Q. Y., 2008, Tropical Plant Biology, V1, P49, DOI 10.1007/s12042-007-9005-7	59	8	8	1	31	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0167-4412	1573-5028		PLANT MOL BIOL	Plant Mol.Biol.	MAR	2016	90	4-5					359	373		10.1007/s11103-015-0422-y			15	Biochemistry & Molecular Biology; Plant Sciences	Biochemistry & Molecular Biology; Plant Sciences	DF2GI	WOS:000371160200003	26694866				2021-04-07	
J	Tapia, JE; Gonzalez, B; Goulitquer, S; Potin, P; Correa, JA				Tapia, Javier E.; Gonzalez, Bernardo; Goulitquer, Sophie; Potin, Philippe; Correa, Juan A.			Microbiota Influences Morphology and Reproduction of the Brown Alga Ectocarpus sp.	FRONTIERS IN MICROBIOLOGY			English	Article						microbiota; bacteria-algae interaction; Ectocarpus; bacterial isolate; algal morphology; exometabolome	ULVA-PERTUSA CHLOROPHYTA; KELP LAMINARIA-DIGITATA; SILICULOSUS ECTOCARPALES; BACTERIAL COMMUNITY; MORPHOGENESIS; PHAEOPHYCEAE; GROWTH; HOST; ARABIDOPSIS; MACROALGAE	Associated microbiota play crucial roles in health and disease of higher organisms. For macroalgae, some associated bacteria exert beneficial effects on nutrition, morphogenesis and growth. However, current knowledge on macroalgae microbiota interactions is mostly based on studies on green and red seaweeds. In this study, we report that when cultured under axenic conditions, the filamentous brown algal model Ectocarpus sp. loses its branched morphology and grows with a small ball like appearance. Nine strains of periphytic bacteria isolated from Ectocarpus sp. unialgal cultures were identified by 16S rRNA sequencing, and assessed for their effect on morphology, reproduction and the metabolites secreted by axenic Ectocarpus sp. Six of these isolates restored morphology and reproduction features of axenic Ectocarpus sp. Bacteria-algae co-culture supernatants, but not the supernatant of the corresponding bacterium growing alone, also recovered morphology and reproduction of the alga. Furthermore, colonization of axenic Ectocarpus sp. with a single bacterial isolate impacted significantly the metabolites released by the alga. These results show that the branched typical morphology and the individuals produced by Ectocarpus sp. are strongly dependent on the presence of bacteria, while the bacterial effect on the algal exometabolome profile reflects the impact of bacteria on the whole physiology of this alga.	[Tapia, Javier E.; Correa, Juan A.] Univ Paris 06, CNRS, Biol & Ecol Algae, Stn Biol Roscoff, Roscoff, France; [Tapia, Javier E.; Correa, Juan A.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Alameda 340, Santiago, Chile; [Gonzalez, Bernardo] Univ Adolfo Ibanez, Ctr Appl Ecol & Sustainabi, Fac Ingn & Ciencias, Santiago, Chile; [Goulitquer, Sophie] Univ Paris 06, MetaboMer Mass Spectrometry Core Facil, CNRS, FR2424,Stn Biol Roscoff, Roscoff, France; [Potin, Philippe] Univ Paris 06, CNRS UMR 8227, Integrat Biol Marine Allodels, Stn Biol Roscoff, Roscoff, France	Correa, JA (corresponding author), Univ Paris 06, CNRS, Biol & Ecol Algae, Stn Biol Roscoff, Roscoff, France.; Correa, JA (corresponding author), Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Alameda 340, Santiago, Chile.	jcorrea@bio.puc.cl			French Government via the National Research Agency in investment expenditure program IDLALG [ANR-10-BTBR-01]; CONICYT PhD scholarshipComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) [21110796]	This work benefited from the support of the French Government via the National Research Agency in investment expenditure program IDLALG (ANR-10-BTBR-01). JT was supported by a CONICYT PhD scholarship (21110796).	Amin SA, 2015, NATURE, V522, P98, DOI 10.1038/nature14488; Andersen RA, 2004, AM J BOT, V91, P1508, DOI 10.3732/ajb.91.10.1508; Armstrong E, 2000, AQUAT MICROB ECOL, V21, P49, DOI 10.3354/ame021049; Arun A, 2013, NEW PHYTOL, V197, P503, DOI 10.1111/nph.12007; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Burke C, 2011, P NATL ACAD SCI USA, V108, P14288, DOI 10.1073/pnas.1101591108; Burke C, 2011, ISME J, V5, P590, DOI 10.1038/ismej.2010.164; Chisholm JRM, 1996, NATURE, V381, P382, DOI 10.1038/381382a0; Choudhary DK, 2009, MICROBIOL RES, V164, P493, DOI 10.1016/j.micres.2008.08.007; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, PLANT SIGNAL BEHAV, V6, P1858, DOI 10.4161/psb.6.11.17737; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Contreras L, 2008, J PHYCOL, V44, P1315, DOI 10.1111/j.1529-8817.2008.00575.x; CORRE S, 1990, BOT MAR, V33, P515, DOI 10.1515/botm.1990.33.6.515; CORREA JA, 1991, J PHYCOL, V27, P448, DOI 10.1111/j.0022-3646.1991.00448.x; Cryan JF, 2012, NAT REV NEUROSCI, V13, P701, DOI 10.1038/nrn3346; Dittami SM, 2016, ISME J, V10, P51, DOI 10.1038/ismej.2015.104; Dittami SM, 2014, FRONT GENET, V5, DOI 10.3389/fgene.2014.00241; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Fry J., 2000, MICROBIOL TODAY, V27, P4151, DOI [10.1111/j.1574-6968.2010.02000.x, DOI 10.1111/J.1574-6968.2010.02000.X]; Fukui Y, 2014, MICROB ECOL, V68, P556, DOI 10.1007/s00248-014-0423-4; Goecke F, 2012, GAYANA BOT, V69, P376, DOI 10.4067/S0717-66432012000200016; Goulitquer S, 2012, MAR DRUGS, V10, P849, DOI 10.3390/md10040849; Grenville-Briggs L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0024500; Gutierrez-Luna FM, 2010, SYMBIOSIS, V51, P75, DOI 10.1007/s13199-010-0066-2; Handelsman J, 2004, MICROBIOL MOL BIOL R, V68, P669, DOI 10.1128/MMBR.68.4.669-685.2004; Harder T., 2009, MARINE EPIBIOSIS CON; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hengst MB, 2010, MICROB ECOL, V60, P282, DOI 10.1007/s00248-010-9647-0; Hollants J, 2013, FEMS MICROBIOL ECOL, V83, P1, DOI 10.1111/j.1574-6941.2012.01446.x; Joint I, 2007, PHILOS T R SOC B, V362, P1223, DOI 10.1098/rstb.2007.2047; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Lachnit T, 2011, ENVIRON MICROBIOL, V13, P655, DOI 10.1111/j.1462-2920.2010.02371.x; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Le Bail A, 2010, PLANT PHYSIOL, V153, P128, DOI 10.1104/pp.109.149708; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lee YK, 2010, SCIENCE, V330, P1768, DOI 10.1126/science.1195568; Littman DR, 2011, CELL HOST MICROBE, V10, P311, DOI 10.1016/j.chom.2011.10.004; Macel M, 2010, MOL ECOL RESOUR, V10, P583, DOI 10.1111/j.1755-0998.2010.02854.x; Marshall K, 2006, MICROB ECOL, V52, P302, DOI 10.1007/s00248-006-9060-x; MARUYAMA A, 1986, J APPL BACTERIOL, V61, P569, DOI 10.1111/j.1365-2672.1986.tb01731.x; Maruyama A., 1990, B JPN SOC MICROBIOL, V5, P1, DOI [10.1264/microbes1986.5.1, DOI 10.1264/MICROBES1986.5.1]; Matsuo Y, 2005, SCIENCE, V307, P1598, DOI 10.1126/science.1105486; Matsuo Y, 2003, ENVIRON MICROBIOL, V5, P25, DOI 10.1046/j.1462-2920.2003.00382.x; Mayer EA, 2011, NAT REV NEUROSCI, V12, P453, DOI 10.1038/nrn3071; Meldau DG, 2013, PLANT CELL, V25, P2731, DOI 10.1105/tpc.113.114744; Muller DG, 2008, CAH BIOL MAR, V49, P59; Nakanishi K, 1999, MAR BIOTECHNOL, V1, P107, DOI 10.1007/PL00011744; Nakanishi K, 1996, J PHYCOL, V32, P479, DOI 10.1111/j.0022-3646.1996.00479.x; Newton AC, 2010, TRENDS MICROBIOL, V18, P365, DOI 10.1016/j.tim.2010.06.002; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; PEDERSEN M, 1968, NATURE, V218, P776, DOI 10.1038/218776a0; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; PROVASOLI L, 1980, J PHYCOL, V16, P196, DOI 10.1111/j.1529-8817.1980.tb03019.x; Qian PY, 2007, MAR BIOTECHNOL, V9, P399, DOI 10.1007/s10126-007-9001-9; Ravanko O, 1970, NOVA HEDWIGIA, V20, P79; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Schroeder FC, 2006, P NATL ACAD SCI USA, V103, P15497, DOI 10.1073/pnas.0605921103; Singh RP, 2014, FEMS MICROBIOL ECOL, V88, P213, DOI 10.1111/1574-6941.12297; Singh RP, 2011, FEMS MICROBIOL ECOL, V76, P381, DOI 10.1111/j.1574-6941.2011.01057.x; Smith CA, 2006, ANAL CHEM, V78, P779, DOI 10.1021/ac051437y; Spoerner M, 2012, J PHYCOL, V48, P1433, DOI 10.1111/j.1529-8817.2012.01231.x; Stirk WA, 2003, PLANT GROWTH REGUL, V41, P13, DOI 10.1023/A:1027376507197; Suzuki MT, 1997, APPL ENVIRON MICROB, V63, P983, DOI 10.1128/AEM.63.3.983-989.1997; Van der Ent S, 2009, PHYTOCHEMISTRY, V70, P1581, DOI 10.1016/j.phytochem.2009.06.009; Van Oosten VR, 2008, MOL PLANT MICROBE IN, V21, P919, DOI 10.1094/MPMI-21-7-0919; Weinberger F, 2007, BIOL BULL-US, V213, P290, DOI 10.2307/25066646; Weinberger F, 2007, J PHYCOL, V43, P235, DOI 10.1111/j.1529-8817.2007.00329.x; WEISBURG WG, 1991, J BACTERIOL, V173, P697, DOI 10.1128/JB.173.2.697-703.1991; Whitman WB, 1998, P NATL ACAD SCI USA, V95, P6578, DOI 10.1073/pnas.95.12.6578; Yang J, 2009, TRENDS PLANT SCI, V14, P1, DOI 10.1016/j.tplants.2008.10.004; Zhang HM, 2008, PLANT J, V56, P264, DOI 10.1111/j.1365-313X.2008.03593.x	76	24	24	1	49	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-302X			FRONT MICROBIOL	Front. Microbiol.	FEB 24	2016	7								197	10.3389/fmicb.2016.00107			14	Microbiology	Microbiology	DE6QZ	WOS:000370760000001	26941722	DOAJ Gold, Green Published			2021-04-07	
J	Strittmatter, M; Grenville-Briggs, LJ; Breithut, L; van West, P; Gachon, CMM; Kupper, FC				Strittmatter, Martina; Grenville-Briggs, Laura J.; Breithut, Lisa; van West, Pieter; Gachon, Claire M. M.; Kuepper, Frithjof C.			Infection of the brown alga Ectocarpus siliculosus by the oomycete Eurychasma dicksonii induces oxidative stress and halogen metabolism	PLANT CELL AND ENVIRONMENT			English	Article						model brown alga; reactive oxygen species; vanadium-dependent bromoperoxidase	LAMINARIA-DIGITATA PHAEOPHYCEAE; HOST SPECIES SUSCEPTIBILITY; SEQUENCE TAG ANALYSIS; REAL-TIME PCR; PHYTOPHTHORA-INFESTANS; APPRESSORIUM FORMATION; PYLAIELLA-LITTORALIS; RED ALGAE; VANADIUM BROMOPEROXIDASE; LIPOPROTEIN PARTICLES	Pathogens are increasingly being recognized as key evolutionary and ecological drivers in marine ecosystems. Defence mechanisms of seaweeds, however, have mostly been investigated by mimicking infection using elicitors. We have established an experimental pathosystem between the genome brown model seaweed Ectocarpus siliculosus and the oomycete Eurychasma dicksonii as a powerful new tool to investigate algal responses to infection. Using proteomics, we identified 21 algal proteins differentially accumulated in response to Eu. dicksonii infection. These include classical algal stress response proteins such as a manganese superoxide dismutase, heat shock proteins 70 and a vanadium bromoperoxidase. Transcriptional profiling by qPCR confirmed the induction of the latter during infection. The accumulation of hydrogen peroxide was observed at different infection stages via histochemical staining. Inhibitor studies confirmed that the main source of hydrogen peroxide is superoxide converted by superoxide dismutase. Our data give an unprecedented global overview of brown algal responses to pathogen infection, and highlight the importance of oxidative stress and halogen metabolism in these interactions. This suggests overlapping defence pathways with herbivores and abiotic stresses. We also identify previously unreported actors, in particular a Rad23 and a plastid-lipid-associated protein, providing novel insights into the infection and defence processes in brown algae.	[Strittmatter, Martina; Gachon, Claire M. M.; Kuepper, Frithjof C.] Scottish Assoc Marine Sci, Scottish Marine Inst, Oban PA37 1QA, Argyll, Scotland; [Strittmatter, Martina; Grenville-Briggs, Laura J.; van West, Pieter] Univ Aberdeen, Inst Med Sci, Aberdeen Oomycete Lab, Foresterhill, Aberdeen AB25 2ZD, Scotland; [Grenville-Briggs, Laura J.] Swedish Univ Agr Sci, Dept Plant Protect Biol, S-23053 Alnarp, Sweden; [Breithut, Lisa] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany; [Kuepper, Frithjof C.] Univ Aberdeen, Oceanlab, Main St, Newburgh AB41 6AA, Scotland	Kupper, FC (corresponding author), Scottish Assoc Marine Sci, Scottish Marine Inst, Oban PA37 1QA, Argyll, Scotland.; Kupper, FC (corresponding author), Univ Aberdeen, Oceanlab, Main St, Newburgh AB41 6AA, Scotland.	fkuepper@abdn.ac.uk		van West, Pieter/0000-0002-0767-6017; Kuepper, Frithjof/0000-0003-1273-7109; Strittmatter, Martina/0000-0002-1258-9751; Gachon, Claire/0000-0002-3702-7472	European Commission (ECOSUMMER) [MEST-CT-2005-20501]; FEMS/ESCMID; Genomia Fund; Marie Curie postdoctoral fellowship from the UK Natural Environment Research Council (NERC) [MEIF-CT-2006-022837]; Marie Curie Re-Integration Grant from the UK Natural Environment Research Council (NERC) [PERG03-GA-2008-230865]; New Investigator grant from the UK Natural Environment Research Council (NERC) [NE/J00460X/1]; NERCUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/D521522/1, NE/F012705/1, Oceans 2025 / WP 4.5, NE/F012578/1]; MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland - the Scottish Funding Council); MASTS pooling initiative [HR09011]; TOTAL Foundation (Paris); Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NBAF010002, NE/F012705/1, NE/J00460X/1, NE/F012578/1, NE/D521522/1, dml010007] Funding Source: researchfish	We would like to thank the Aberdeen Proteome Facility, especially Phil Cash, David Stead and Evelyn Argo for assistance with 2D electrophoresis and mass spectrometry. M.S. gratefully acknowledges a Marie Curie PhD fellowship from the European Commission (ECOSUMMER, MEST-CT-2005-20501), a joint FEMS/ESCMID Research Fellowship and the Genomia Fund. C.M.M.G. is supported by a Marie Curie postdoctoral fellowship (MEIF-CT-2006-022837), a Marie Curie Re-Integration Grant (PERG03-GA-2008-230865) and a New Investigator grant from the UK Natural Environment Research Council (NERC, grant NE/J00460X/1). F.C.K. would like to thank NERC for funding (grants NE/D521522/1, NE/F012705/1 and Oceans 2025 / WP 4.5). L.J.G.-B., C.M.M.G., F.C.K. and P.W. would like to acknowledge funding from NERC for a Strategic Ocean Funding Initiative award (NE/F012578/1). Funding from the MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) and from the TOTAL Foundation (Paris) to F.C.K. is gratefully acknowledged. Finally, we would like to thank the two anonymous referees for constructive suggestions to improve our manuscript.	Asada K, 2006, PLANT PHYSIOL, V141, P391, DOI 10.1104/pp.106.082040; Austin JR, 2006, PLANT CELL, V18, P1693, DOI 10.1105/tpc.105.039859; Bhadauria V, 2010, EUR J PLANT PATHOL, V126, P81, DOI 10.1007/s10658-009-9521-4; Bouarab K, 1999, PLANT CELL, V11, P1635, DOI 10.1105/tpc.11.9.1635; Brehelin C, 2007, TRENDS PLANT SCI, V12, P260, DOI 10.1016/j.tplants.2007.04.003; Butler A, 2004, NAT PROD REP, V21, P180, DOI 10.1039/b302337k; Chen J, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-414; Chen L, 2002, MOL CELL BIOL, V22, P4902, DOI 10.1128/MCB.22.13.4902-4913.2002; Cock JM, 2012, GENES GENOM, V34, P1, DOI 10.1007/s13258-010-0225-0; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; Contreras L, 2008, J PHYCOL, V44, P1315, DOI 10.1111/j.1529-8817.2008.00575.x; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; Crepineau F, 2000, PLANT MOL BIOL, V43, P503, DOI 10.1023/A:1006489920808; Dantuma NP, 2009, DNA REPAIR, V8, P449, DOI 10.1016/j.dnarep.2009.01.005; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Farmer LM, 2010, PLANT CELL, V22, P124, DOI 10.1105/tpc.109.072660; Farnham G, 2013, J PHYCOL, V49, P819, DOI 10.1111/jpy.12096; Fosu-Nyarko J, 2009, MOL PLANT PATHOL, V10, P237, DOI 10.1111/j.1364-3703.2008.00526.x; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Goulitquer S, 2009, CHEMBIOCHEM, V10, P977, DOI 10.1002/cbic.200900004; Grenville-Briggs L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0024500; Grenville-Briggs LJ, 2008, PLANT CELL, V20, P720, DOI 10.1105/tpc.107.052043; Grenville-Briggs LJ, 2010, FUNGAL BIOL-UK, V114, P702, DOI 10.1016/j.funbio.2010.06.003; Grenville-Briggs LJ, 2005, FUNGAL GENET BIOL, V42, P244, DOI 10.1016/j.fgb.2004.11.009; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Heesch Svenja, 1999, Phycological Research, V47, P1; Kav Nat N. V., 2007, Current Proteomics, V4, P28; Klochkova TA, 2012, J APPL PHYCOL, V24, P135, DOI 10.1007/s10811-011-9661-8; Konotchick T, 2013, NEW PHYTOL, V198, P398, DOI 10.1111/nph.12160; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; Kupper FC, 2014, J PHYCOL, V50, P652, DOI 10.1111/jpy.12199; Kupper FC, 2013, J EXP BOT, V64, P2653, DOI 10.1093/jxb/ert110; Kupper FC, 2009, PLANT CELL PHYSIOL, V50, P789, DOI 10.1093/pcp/pcp023; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; La Barre S, 2010, MAR DRUGS, V8, P988, DOI 10.3390/md8040988; Langenkamper G, 2001, J EXP BOT, V52, P1545, DOI 10.1093/jexbot/52.360.1545; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Mators A, 2013, FRONT PLANT SCI, V4, DOI 10.3389/fpls.2013.00089; Mayer MP, 2005, CELL MOL LIFE SCI, V62, P670, DOI 10.1007/s00018-004-4464-6; Mithofer A, 2004, FEBS LETT, V566, P1, DOI 10.1016/j.febslet.2004.04.011; Muller DG, 2008, CAH BIOL MAR, V49, P59; Nelson MM, 2008, INFECT IMMUN, V76, P828, DOI 10.1128/IAI.01115-07; Orozco-Cardenas ML, 2001, PLANT CELL, V13, P179, DOI 10.1105/tpc.13.1.179; Palmer CJ, 2005, ENVIRON CHEM, V2, P282, DOI 10.1071/EN05078; Park CJ, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00177; Paul NA, 2006, MAR ECOL PROG SER, V306, P87, DOI 10.3354/meps306087; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Pfaffl MW, 2002, NUCLEIC ACIDS RES, V30, DOI 10.1093/nar/30.9.e36; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Roy N, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0009796; Salgado LT, 2009, J PHYCOL, V45, P193, DOI 10.1111/j.1529-8817.2008.00642.x; Santino A, 2013, PLANT CELL REP, V32, P1085, DOI 10.1007/s00299-013-1441-2; Schmidt F, 2011, PROTEOMICS, V11, P3203, DOI 10.1002/pmic.201100158; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Singh DK, 2010, PLANT PHYSIOL, V154, P1281, DOI 10.1104/pp.110.164095; Strittmatter M, 2009, OOMYCETE GENETICS GE, P25; Strittmatter M, 2013, DIS AQUAT ORGAN, V104, P1, DOI 10.3354/dao02583; Tan KC, 2009, MOL PLANT PATHOL, V10, P703, DOI [10.1111/J.1364-3703.2009.00565.X, 10.1111/j.1364-3703.2009.00565.x]; Thomas F, 2014, NEW PHYTOL, V204, P567, DOI 10.1111/nph.12925; Thomas F, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0021475; Tonon T, 2008, J PHYCOL, V44, P1250, DOI 10.1111/j.1529-8817.2008.00580.x; Tsirigoti A, 2014, PLANT BIOLOGY, V16, P272, DOI 10.1111/plb.12041; Tsirigoti A, 2013, PLANT SIGNAL BEHAV, V8, DOI 10.4161/psb.26367; Uppalapati SR, 2000, J PHYCOL, V36, P359, DOI 10.1046/j.1529-8817.2000.99099.x; Vidi PA, 2006, J BIOL CHEM, V281, P11225, DOI 10.1074/jbc.M511939200; Vizcaino JA, 2013, NUCLEIC ACIDS RES, V41, pD1063, DOI 10.1093/nar/gks1262; Weinberger F, 2000, J APPL PHYCOL, V12, P139, DOI 10.1023/A:1008119125911; Weinberger F, 2005, J EXP BOT, V56, P1317, DOI 10.1093/jxb/eri132; Weinberger F, 2007, BIOL BULL-US, V213, P290, DOI 10.2307/25066646; West JA, 2006, PHYCOL RES, V54, P72, DOI 10.1111/j.1440-1835.2006.00410.x; West JA, 1999, HYDROBIOLOGIA, V399, P101; Yang Y, 2006, P NATL ACAD SCI USA, V103, P6061, DOI 10.1073/pnas.0501720103; Youssef A, 2010, PLANT J, V61, P436, DOI 10.1111/j.1365-313X.2009.04067.x; Ytterberg AJ, 2006, PLANT PHYSIOL, V140, P984, DOI 10.1104/pp.105.076083; Zambounis A, 2013, AQUAT BOT, V104, P147, DOI 10.1016/j.aquabot.2012.07.008; Zhou WC, 2006, PROTEOMICS, V6, P4599, DOI 10.1002/pmic.200600052	85	15	17	0	24	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0140-7791	1365-3040		PLANT CELL ENVIRON	Plant Cell Environ.	FEB	2016	39	2					259	271		10.1111/pce.12533			13	Plant Sciences	Plant Sciences	DD7XC	WOS:000370137300004	25764246	Green Published, Other Gold			2021-04-07	
J	Ryu, YH; Yeo, KB; Ki, MR; Kim, YJ; Pack, SP				Ryu, Young Ha; Yeo, Ki Baek; Ki, Mi-Ran; Kim, Yong Jun; Pack, Seung Pil			Improved stability and reusability of endoglucanase from Clostridium thermocellum by a biosilica-based auto-encapsulation method	BIOCHEMICAL ENGINEERING JOURNAL			English	Article						Cellulase; Immobilised enzymes; Immobilization; Polypeptides; Biosilicification; Silica forming peptide	ENZYME IMMOBILIZATION; SILICA; PROTEIN	The functional improvement of endoglucanase (EG), a key cellulose-hydrolyzing biocatalyst, is imperative for the practical use of cellulosic materials such as lignocellulose, stove and straws. Here, we employed a bio-inspired silica-encapsulation method to improve the stability and reusability of EG. We introduced a new silica-forming peptide (SFP) from Ectocarpus siliculosus at the C-terminus of EG to generate a recombinant fusion protein, EG-SFP, with auto-silicifying ability. We obtained an EG-SFP-encapsulated silica matrix (EG-SFP@Silica) via the EG-SFP-mediated auto-silicification process under ambient conditions. The immobilization efficiency was 90%. The introduction of SFP did not significantly affect the functionality of EG, and moreover, EG-SFP@Silica demonstrated higher thermostability by 5 degrees C than free EG-SFP or EG. In addition, EG-SFP@Silica retained 90% of its initial residual activity with up to 18 uses. These results provide a platform for the development of a practical enzymatic hydrolysis process for cellulosic materials. (C) 2015 Elsevier B.V. All rights reserved.	[Ryu, Young Ha; Yeo, Ki Baek; Ki, Mi-Ran; Pack, Seung Pil] Korea Univ, Dept Biotechnol & Bioinformat, Sejong 339700, South Korea; [Kim, Yong Jun] Korea Univ, Dept Food & Biotechnol, Sejong 339700, South Korea	Pack, SP (corresponding author), Korea Univ, Dept Food & Biotechnol, Sejong 339700, South Korea.	spack@korea.ac.kr			Marine Biotechnology Program (Marine BioMaterials Research Center) - Ministry of Oceans and Fisheries, Korea; Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Korea [2013R1A1A2008759, 2013R1A1A2010824]	This work was supported by the Marine Biotechnology Program (Marine BioMaterials Research Center), funded by the Ministry of Oceans and Fisheries, Korea and was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Korea (2013R1A1A2008759, 2013R1A1A2010824).	CHAKRABARTI AC, 1988, APPL BIOCHEM BIOTECH, V19, P189, DOI 10.1007/BF02921483; Chang RHY, 2011, GREEN CHEM, V13, P2844, DOI 10.1039/c1gc15563f; Daniel K.M., 2012, BIOFUELS BIOTECHNOL, V109, P1083; Foo CWP, 2004, TRENDS BIOTECHNOL, V22, P577, DOI 10.1016/j.tibtech.2004.09.011; Hwang ET, 2013, ENG LIFE SCI, V13, P49, DOI 10.1002/elsc.201100225; Khoshnevisan K, 2011, CHEM ENG J, V171, P669, DOI 10.1016/j.cej.2011.04.039; Ki M.R., 2013, 2013 INT C BIOL BIOM, P39; Ki MR, 2013, BIOPROC BIOSYST ENG, V36, P643, DOI 10.1007/s00449-012-0818-x; Kostylev M, 2012, BIOFUELS-UK, V3, P61, DOI 10.4155/BFS.11.150; Lechner CC, 2014, J PEPT SCI, V20, P152, DOI 10.1002/psc.2577; Lechner CC, 2013, BIOORGAN MED CHEM, V21, P3533, DOI 10.1016/j.bmc.2013.04.006; LOBARZEWSKI J, 1989, ACTA BIOTECHNOL, V9, P239, DOI 10.1002/abio.370090310; Marner WD, 2009, BIOTECHNOL PROGR, V25, P417, DOI 10.1002/btpr.136; Mateo C, 2007, ENZYME MICROB TECH, V40, P1451, DOI 10.1016/j.enzmictec.2007.01.018; MILLER GL, 1959, ANAL CHEM, V31, P426, DOI 10.1021/ac60147a030; Mohanram S., 2013, SUSTAIN CHEM PROCESS, P1; Nam DH, 2009, BIOTECHNOL PROGR, V25, P1643, DOI 10.1002/btpr.261; Pamirsky IE, 2013, MAR DRUGS, V11, P3155, DOI 10.3390/md11093155; Puri M, 2013, TRENDS BIOTECHNOL, V31, P215, DOI 10.1016/j.tibtech.2013.01.002; SCHWARZ WH, 1986, APPL ENVIRON MICROB, V51, P1293, DOI 10.1128/AEM.51.6.1293-1299.1986; Wyman CE, 2001, APPL BIOCHEM BIOTECH, V91-3, P5, DOI 10.1385/ABAB:91-93:1-9:5; Xie Y, 2010, APPL BIOCHEM BIOTECH, V160, P1074, DOI 10.1007/s12010-009-8559-2; Yennamalli RM, 2013, BIOTECHNOL BIOFUELS, V6, DOI 10.1186/1754-6834-6-136; Yeoman CJ, 2010, ADV APPL MICROBIOL, V70, P1, DOI 10.1016/S0065-2164(10)70001-0; Yin Z.S. Hailei, 2013, AFR J MICROBIOL RES, V7, P3248, DOI DOI 10.5897/AJMR12.1947	25	11	11	1	26	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	1369-703X	1873-295X		BIOCHEM ENG J	Biochem. Eng. J.	JAN 15	2016	105		A				144	149		10.1016/j.bej.2015.09.006			6	Biotechnology & Applied Microbiology; Engineering, Chemical	Biotechnology & Applied Microbiology; Engineering	DA4NJ	WOS:000367776300016					2021-04-07	
J	Lipinska, AP; Van Damme, EJM; De Clerck, O				Lipinska, Agnieszka P.; Van Damme, Els J. M.; De Clerck, Olivier			Molecular evolution of candidate male reproductive genes in the brown algal model Ectocarpus	BMC EVOLUTIONARY BIOLOGY			English	Article						Cell-cell recognition; Gamete receptor; Ectocarpus; Brown algae; Fertilization	SEX-BIASED GENES; PHAEOPHYCEAE SPERM RECEPTOR; POSITIVE SELECTION; GAMETE RECOGNITION; RAPID EVOLUTION; MAXIMUM-LIKELIHOOD; PHYLOGENETIC ANALYSIS; URCHIN RECEPTOR; EGG RECEPTOR; PROTEIN	Background: Evolutionary studies of genes that mediate recognition between sperm and egg contribute to our understanding of reproductive isolation and speciation. Surface receptors involved in fertilization are targets of sexual selection, reinforcement, and other evolutionary forces including positive selection. This observation was made across different lineages of the eukaryotic tree from land plants to mammals, and is particularly evident in free-spawning animals. Here we use the brown algal model species Ectocarpus (Phaeophyceae) to investigate the evolution of candidate gamete recognition proteins in a distant major phylogenetic group of eukaryotes. Results: Male gamete specific genes were identified by comparing transcriptome data covering different stages of the Ectocarpus life cycle and screened for characteristics expected from gamete recognition receptors. Selected genes were sequenced in a representative number of strains from distant geographical locations and varying stages of reproductive isolation, to search for signatures of adaptive evolution. One of the genes (Esi0130_0068) showed evidence of selective pressure. Interestingly, that gene displayed domain similarities to the receptor for egg jelly (REJ) protein involved in sperm-egg recognition in sea urchins. Conclusions: We have identified a male gamete specific gene with similarity to known gamete recognition receptors and signatures of adaptation. Altogether, this gene could contribute to gamete interaction during reproduction as well as reproductive isolation in Ectocarpus and is therefore a good candidate for further functional evaluation.	[Lipinska, Agnieszka P.; De Clerck, Olivier] Univ Ghent, Phycol Res Grp, B-9000 Ghent, Belgium; [Lipinska, Agnieszka P.; De Clerck, Olivier] Univ Ghent, Ctr Mol Phylogenet & Evolut, B-9000 Ghent, Belgium; [Van Damme, Els J. M.] Univ Ghent, Lab Biochem & Glycobiol, Dept Mol Biotechnol, B-9000 Ghent, Belgium	Lipinska, AP (corresponding author), Univ Ghent, Phycol Res Grp, Krijgslaan 281,Bldg S8, B-9000 Ghent, Belgium.	ap.lipinska@gmail.com	De Clerck, Olivier/ABC-2683-2020; De Clerck, Olivier/A-9083-2010; Van Damme, Els/U-3904-2019; Van Damme, Els J/B-4410-2015; De Clerck, Olivier/AAU-4295-2020	De Clerck, Olivier/0000-0002-3699-8402; De Clerck, Olivier/0000-0002-3699-8402; Van Damme, Els/0000-0001-9848-766X; 	BOF Grant, Ghent University, Belgium [09/24 J/117]	This work was funded by BOF Grant 09/24 J/117, Ghent University, Belgium. We gratefully acknowledge Dieter Muller and Akira Peters for providing the Ectocarpus strain used in this study and for instructions on culturing. We thank Susana Coelho and Mark Cock for helpful discussions.	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Armbrust EV, 1999, APPL ENVIRON MICROB, V65, P3121; Armbrust EV, 2001, APPL ENVIRON MICROB, V67, P3501, DOI 10.1128/AEM.67.8.3501-3513.2001; Bernasconi G, 2004, SCIENCE, V303, P971, DOI 10.1126/science.1092180; Blackman LM, 2011, PROTIST, V162, P100, DOI 10.1016/j.protis.2010.01.005; BOLWELL GP, 1980, J CELL SCI, V43, P209; BOLWELL GP, 1979, J CELL SCI, V36, P19; Callow JA, 1992, SOC EXPT BIOL SEMINA; Chapman T, 2006, CURR BIOL, V16, pR744, DOI 10.1016/j.cub.2006.08.020; Civetta A, 1995, J MOL EVOL, V41, P1085, DOI 10.1007/BF00173190; Civetta A, 1998, MOL BIOL EVOL, V15, P901, DOI 10.1093/oxfordjournals.molbev.a025994; Clark NL, 2009, PLOS GENET, V5, DOI 10.1371/journal.pgen.1000570; Clark NL, 2005, PLOS GENET, V1, P335, DOI 10.1371/journal.pgen.0010035; Clark NL, 2006, REPRODUCTION, V131, P11, DOI 10.1530/rep.1.00357; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Conesa A, 2005, BIOINFORMATICS, V21, P3674, DOI 10.1093/bioinformatics/bti610; Delport W, 2010, BIOINFORMATICS, V26, P2455, DOI 10.1093/bioinformatics/btq429; Dobzhansky T., 1951, GENETICS ORIGIN SPEC; Ellegren H, 2007, NAT REV GENET, V8, P689, DOI 10.1038/nrg2167; FELSENSTEIN J, 1981, J MOL EVOL, V17, P368, DOI 10.1007/BF01734359; Ferris PJ, 1997, P NATL ACAD SCI USA, V94, P8634, DOI 10.1073/pnas.94.16.8634; Galindo BE, 2003, P NATL ACAD SCI USA, V100, P4639, DOI 10.1073/pnas.0830022100; Gossmann TI, 2014, MOL BIOL EVOL, V31, P574, DOI 10.1093/molbev/mst226; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Gunaratne HJ, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-235; Hamm D, 2007, AM J HUM GENET, V81, P44, DOI 10.1086/518695; Hart MW, 2014, EVOLUTION, V68, P1294, DOI 10.1111/evo.12352; Hart MW, 2012, EVOLUTION, V66, P1675, DOI 10.1111/j.1558-5646.2012.01605.x; Hellberg ME, 1999, MOL BIOL EVOL, V16, P839, DOI 10.1093/oxfordjournals.molbev.a026168; Hirohashi N, 2008, DEV GROWTH DIFFER, V50, pS221, DOI 10.1111/j.1440-169X.2008.01017.x; Honda D, 2007, PROTIST, V158, P77, DOI 10.1016/j.protis.2006.08.004; Hughes J, 1999, HUM MOL GENET, V8, P543, DOI 10.1093/hmg/8.3.543; Kelley LA, 2009, NAT PROTOC, V4, P363, DOI 10.1038/nprot.2009.2; Koressaar T, 2007, BIOINFORMATICS, V23, P1289, DOI 10.1093/bioinformatics/btm091; Kosman ET, 2014, MOL HUM REPROD, V20, P1190, DOI 10.1093/molehr/gau069; Krogh A, 2001, J MOL BIOL, V305, P567, DOI 10.1006/jmbi.2000.4315; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; LEE YH, 1995, MOL BIOL EVOL, V12, P231; Lessios HA, 2012, MOL ECOL, V21, P130, DOI 10.1111/j.1365-294X.2011.05303.x; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Lipinska AP, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-909; Mah SA, 2005, MOL BIOL EVOL, V22, P533, DOI 10.1093/molbev/msi037; Mank JE, 2007, MOL BIOL EVOL, V24, P2698, DOI 10.1093/molbev/msm208; Mank JE, 2009, BIOL LETTERS, V5, P409, DOI 10.1098/rsbl.2008.0732; Metz EC, 1996, MOL BIOL EVOL, V13, P397, DOI 10.1093/oxfordjournals.molbev.a025598; Moy GW, 1996, J CELL BIOL, V133, P809, DOI 10.1083/jcb.133.4.809; Mugal CF, 2014, MOL BIOL EVOL, V31, P212, DOI 10.1093/molbev/mst192; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; Murrell B, 2012, PLOS GENET, V8, DOI 10.1371/journal.pgen.1002764; Nydam ML, 2011, BMC EVOL BIOL, V11, DOI 10.1186/1471-2148-11-18; Palumbi SR, 2009, HEREDITY, V102, P66, DOI 10.1038/hdy.2008.104; Parfrey LW, 2011, P NATL ACAD SCI USA, V108, P13624, DOI 10.1073/pnas.1110633108; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Pond SLK, 2005, BIOINFORMATICS, V21, P676, DOI 10.1093/bioinformatics/bti079; Qian F, 2002, P NATL ACAD SCI USA, V99, P16981, DOI 10.1073/pnas.252484899; Rambaut A, 2009, TRACER V1 5; Ronquist F, 2012, SYST BIOL, V61, P539, DOI 10.1093/sysbio/sys029; SCHMID CE, 1994, PLANT SCI, V102, P61, DOI 10.1016/0168-9452(94)90021-3; SCHMID CE, 1993, HYDROBIOLOGIA, V261, P437; Sonnhammer E L, 1998, Proc Int Conf Intell Syst Mol Biol, V6, P175; Sorhannus U, 2003, MOL BIOL EVOL, V20, P1326, DOI 10.1093/molbev/msg145; Sorhannus U, 2006, J MOL EVOL, V63, P231, DOI 10.1007/s00239-006-0016-z; Stache B, 1990, EVOLUTIONARY BIOGE G, VG22, P173; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Stamatakis A, 2006, PAR DISTR PROC S 200; Stamatakis A, 2008, SYST BIOL, V57, P758, DOI 10.1080/10635150802429642; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Swanson WJ, 1998, SCIENCE, V281, P710, DOI 10.1126/science.281.5377.710; Swanson WJ, 2001, P NATL ACAD SCI USA, V98, P2509, DOI 10.1073/pnas.051605998; Swanson WJ, 2002, ANNU REV ECOL SYST, V33, P161, DOI 10.1146/annurev.ecolsys.33.010802.150439; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; TRIMMER JS, 1986, P NATL ACAD SCI USA, V83, P9055, DOI 10.1073/pnas.83.23.9055; Turner LM, 2008, INT J DEV BIOL, V52, P769, DOI 10.1387/ijdb.082577lt; Untergasser A, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gks596; Vacquier VD, 1997, DEV BIOL, V192, P125, DOI 10.1006/dbio.1997.8729; Vacquier VD, 2011, CSH PERSPECT BIOL, V3, DOI 10.1101/cshperspect.a002931; Villesen P, 2007, MOL ECOL NOTES, V7, P965, DOI 10.1111/j.1471-8286.2007.01821.x; Wang X, 2001, PLANT PHYSIOL, V125, P1012, DOI 10.1104/pp.125.2.1012; WRIGHT PJ, 1995, J PHYCOL, V31, P584, DOI 10.1111/j.1529-8817.1995.tb02554.x; WRIGHT PJ, 1995, J PHYCOL, V31, P592, DOI 10.1111/j.1529-8817.1995.tb02555.x; Yang ZH, 2000, J MOL EVOL, V51, P423, DOI 10.1007/s002390010105; Yang ZH, 2000, GENETICS, V155, P431; Yang ZH, 1998, MOL BIOL EVOL, V15, P568, DOI 10.1093/oxfordjournals.molbev.a025957; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088	87	8	8	0	42	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2148			BMC EVOL BIOL	BMC Evol. Biol.	JAN 5	2016	16								5	10.1186/s12862-015-0577-9			11	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	CZ9QB	WOS:000367431000002	26728038	DOAJ Gold, Green Published			2021-04-07	
J	Kinoshita, N; Nagasato, C; Tanaka, A; Motomura, T				Kinoshita, Nana; Nagasato, Chikako; Tanaka, Atsuko; Motomura, Taizo			Chemotaxis in the anisogamous brown alga Mutimo cylindricus (Cutleriaceae, Tilopteridales)	PHYCOLOGIA			English	Article						Anisogamy; Brown alga; Chemotaxis; Flagellar locomotion; Mutimo cylindricus; Sex pheromone; Thigmotaxis	SMITH GREV PHAEOPHYTA; SEX ATTRACTANT; C-HANCOCKII; REPRODUCTION; GAMETOGENESIS; HYDROCARBONS; PHEROMONES; MOVEMENT; GROWTH; PLANT	Locomotion of female and male gametes of Mutimo cylindricus was analysed using a high-speed recording system. Female and male gametes morphologically differed in terms of their cell size and flagellar length. Male gametes swam significantly faster than female gametes; although, the flagellar waveforms were almost the same. Thus, the different swimming velocities of male and female gametes may have been due to differences in their cell size. Chemotactic male gametes treated with the sex pheromone from settled female gametes behaved in a similar manner to the male gametes of the isogamous Ectocarpus siliculosus. The results of the study revealed the following: (1) free-swimming male gametes exhibited thigmotaxis, (2) the beat frequency of the anterior flagellum and the swimming velocity decreased, (3) the deflection angle of the anterior flagellum increased, (4) the unilateral beat of the posterior flagellum was affected by the sex pheromone gradient, and (5) the tracking radius of male gametes around female gametes decreased.	[Kinoshita, Nana] Hokkaido Univ, Grad Sch Environm Sci, Sapporo, Hokkaido 0600810, Japan; [Nagasato, Chikako; Tanaka, Atsuko; Motomura, Taizo] Hokkaido Univ, Muroran Marine Stn, Field Sci Ctr Northern Biosphere, Muroran, Hokkaido 0510013, Japan	Motomura, T (corresponding author), Hokkaido Univ, Muroran Marine Stn, Field Sci Ctr Northern Biosphere, Muroran, Hokkaido 0510013, Japan.	motomura@fsc.hokudai.ac.jp					BOLAND W, 1989, HELV CHIM ACTA, V72, P1288, DOI 10.1002/hlca.19890720616; BRAY D, 1992, CELL MOVEMENTS, P3; CLAYTON MN, 1989, SYST ASSOC SPEC VOL, V38, P229; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Fritsch FE, 1945, STRUCTURE REPROD ALG; GELLER A, 1981, J EXP BIOL, V92, P53; JAENICKE L, 1974, J AM CHEM SOC, V96, P3324, DOI 10.1021/ja00817a056; Kawai H., 1992, KOREAN J PHYCOLOGY, V7, P33; Kinoshita N, 2016, EUR J PHYCOL, V51, P139, DOI 10.1080/09670262.2015.1109144; KITAYAMA T, 1992, PHYCOLOGIA, V31, P449, DOI 10.2216/i0031-8884-31-5-449.1; LACLAIRE JW, 1979, PROTOPLASMA, V101, P247, DOI 10.1007/BF01276967; LACLAIRE JW, 1978, PROTOPLASMA, V97, P93, DOI 10.1007/BF01276686; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MAIER I, 1990, J EXP BOT, V41, P869, DOI 10.1093/jxb/41.7.869; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; MOTOMURA T, 1993, SCI PAP I ALG RES HO, V9, P1; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1974, BIOCHEM PHYSIOL PFL, V165, P212; Nagasato Chikako, 1998, Phycological Research, V46, P191, DOI 10.1111/j.1440-1835.1998.tb00113.x; OSBORN M, 1982, METHOD CELL BIOL, V24, P97; Provasoli L., 1968, CULTURES COLLECTIONS, P63; PURCELL EM, 1977, AM J PHYS, V45, P3, DOI 10.1119/1.10903; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; WYNNE M J, 1976, Phycologia, V15, P435, DOI 10.2216/i0031-8884-15-3-435.1; Yamanouchi S, 1912, BOT GAZ, V54, P0441, DOI 10.1086/330952	26	6	6	0	9	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia		2016	55	4					359	364		10.2216/15-150.1			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	DQ0KT	WOS:000378888700003					2021-04-07	
J	Miller, EP; Auerbach, H; Schunemann, V; Tymon, T; Carrano, CJ				Miller, Eric P.; Auerbach, Hendrik; Schuenemann, Volker; Tymon, Teresa; Carrano, Carl J.			Surface binding, localization and storage of iron in the giant kelp Macrocystis pyrifera	METALLOMICS			English	Article							ECTOCARPUS-SILICULOSUS; MARINE-PHYTOPLANKTON; CELL-SURFACE; BOUND IRON; TRANSLOCATION; DEFICIENCY; PHAEOPHYTA; REDUCTASE; ROOTS; ACID	Iron is an essential element for all living organisms due to its ubiquitous role in redox and other enzymes, especially in the context of respiration and photosynthesis. Although the iron uptake and storage mechanisms of terrestrial/higher plants have been well-studied, the corresponding systems in marine algae have received far less attention. While the iron many marine algae take up from the environment, irrespective of its detailed internalization mechanism, arrives at the cell surface by diffusion, there is growing evidence for more "active" means of concentrating this element prior to uptake. It has been well established in both laboratory and environmentally derived samples, that a large amount of iron can be "non-specifically" adsorbed to the surface of marine algae. While this phenomenon is widely recognized and has prompted the development of experimental protocols to eliminate its contribution to iron uptake studies, its potential biological significance as a concentrated iron storage source for marine algae is only now being recognized. In this study, using an interdisciplinary array of techniques, we show that the giant kelp Macrocystis pyrifera also displays significant cell surface bound iron although less than that seen with the related brown alga Ectocarpus siliculosus. The iron on the surface is likely bound to carboxylate groups and once inside the iron is found to localize differently depending on cell type. Iron appears to be stored in an as yet undefined mineral phase.	[Miller, Eric P.; Tymon, Teresa; Carrano, Carl J.] San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA; [Auerbach, Hendrik; Schuenemann, Volker] Tech Univ Kaiserslautern, Dept Phys, D-67663 Kaiserslautern, Germany	Carrano, CJ (corresponding author), San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA.	ccarrano@mail.sdsu.edu	Schunemann, Volker/C-6603-2016		California State University Council on Ocean Affairs, Science and Technology (COAST); research initiative NANOKAT	The authors are grateful to Dr Steve Barlow and the SDSU Electron Microscope Facility for expert technical assistance and instrument time. EM has been supported by a California State University Council on Ocean Affairs, Science and Technology (COAST) graduate scholarship. VS acknowledges the support by the research initiative NANOKAT.	Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Bottger LH, 2012, J EXP BOT, V63, P5763, DOI 10.1093/jxb/ers225; BRULAND KW, 1991, LIMNOL OCEANOGR, V36, P1555, DOI 10.4319/lo.1991.36.8.1555; DZIOBKOWSKI CT, 1981, INORG CHEM, V20, P671, DOI 10.1021/ic50217a007; FROLOW F, 1994, NAT STRUCT BIOL, V1, P453, DOI 10.1038/nsb0794-453; Giammanco GE, 2015, ACS APPL MATER INTER, V7, P3068, DOI 10.1021/am506772x; Harrison P. M., 1989, IRON CARRIERS IRON P, P123; Hernandez-Carmona G, 2002, J APPL PHYCOL, V14, P445, DOI 10.1023/A:1022372807813; HUDSON RJM, 1989, LIMNOL OCEANOGR, V34, P1113, DOI 10.4319/lo.1989.34.6.1113; Hutchins DA, 1999, AQUAT MICROB ECOL, V19, P129, DOI 10.3354/ame019129; Keppler F, 2003, CHEMOSPHERE, V52, P477, DOI 10.1016/S0045-6535(03)00198-X; Lanquar V, 2005, EMBO J, V24, P4041, DOI 10.1038/sj.emboj.7600864; MANLEY SL, 1984, J PHYCOL, V20, P192, DOI 10.1111/j.0022-3646.1984.00192.x; MANLEY SL, 1983, J PHYCOL, V19, P118, DOI 10.1111/j.0022-3646.1983.00118.x; MARTIN JH, 1988, NATURE, V331, P341, DOI 10.1038/331341a0; Martinoia E, 2007, J EXP BOT, V58, P83, DOI 10.1093/jxb/erl183; Matzanke B. F., 1997, TRANSITION METALS MI; MATZANKE BF, 1987, J BACTERIOL, V169, P5873, DOI 10.1128/jb.169.12.5873-5876.1987; Meguro R, 2007, ARCH HISTOL CYTOL, V70, P1, DOI 10.1679/aohc.70.1; Miller EP, 2014, J EXP BOT, V65, P585, DOI 10.1093/jxb/ert406; Milligan AJ, 2009, MAR CHEM, V114, P31, DOI 10.1016/j.marchem.2009.03.003; MOOG PR, 1994, PLANT SOIL, V165, P241, DOI 10.1007/BF00008068; Morup S., 2011, MOSSBAUER SPECTROSCO, P201; Park MJ, 2014, CHEM COMMUN, V50, P8547, DOI 10.1039/c4cc02132k; PARKER BC, 1965, J PHYCOL, V1, P41, DOI 10.1111/j.1529-8817.1965.tb04554.x; Paz Y, 2007, PLANT PHYSIOL, V144, P1407, DOI 10.1104/pp.107.100644; Robinson NJ, 1999, NATURE, V397, P694, DOI 10.1038/17800; ROMHELD V, 1986, PLANT PHYSIOL, V80, P175, DOI 10.1104/pp.80.1.175; Roschzttardtz H, 2009, PLANT PHYSIOL, V151, P1329, DOI 10.1104/pp.109.144444; Sutak R, 2012, PLANT PHYSIOL, V160, P2271, DOI 10.1104/pp.112.204156; Sutak R, 2010, PLANT PHYSIOL, V154, P991, DOI 10.1104/pp.110.159947; Takeda H, 2011, ENERG ENVIRON SCI, V4, P2575, DOI 10.1039/c1ee01236c; Tovar-Sanchez A, 2003, MAR CHEM, V82, P91, DOI 10.1016/S0304-4203(03)00054-9; van Bergeijk SA, 2013, J PHYCOL, V49, P640, DOI 10.1111/jpy.12073	34	6	6	0	8	ROYAL SOC CHEMISTRY	CAMBRIDGE	THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND	1756-5901	1756-591X		METALLOMICS	Metallomics		2016	8	4					403	411		10.1039/c6mt00027d			9	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	DK3AW	WOS:000374788300003	27009567	Other Gold			2021-04-07	
J	Miller, EP; Wu, YX; Carrano, CJ				Miller, Eric P.; Wu, Youxian; Carrano, Carl J.			Boron uptake, localization, and speciation in marine brown algae	METALLOMICS			English	Article							MACROCYSTIS-PYRIFERA PHAEOPHYTA; LONG-DISTANCE TRANSPORT; BORIC-ACID CHANNEL; GIANT-KELP; B-11 NMR; BORATE; TRANSLOCATION; PLANTS; ARABIDOPSIS; REQUIREMENT	In contrast to the generally boron-poor terrestrial environment, the concentration of boron in the marine environment is relatively high (0.4 mM) and while there has been extensive interest in its use as a surrogate of pH in paleoclimate studies in the context of climate change-related questions, the relatively depth independent, and the generally non-nutrient-like concentration profile of this element have led to boron being neglected as a potentially biologically relevant element in the ocean. Among the marine plant-like organisms the brown algae (Phaeophyta) are one of only five lineages of photosynthetic eukaryotes to have evolved complex multicellularity. Many of unusual and often unique features of brown algae are attributable to this singular evolutionary history. These adaptations are a reflection of the marine coastal environment which brown algae dominate in terms of biomass. Consequently, brown algae are of fundamental importance to oceanic ecology, geochemistry, and coastal industry. Our results indicate that boron is taken up by a facilitated diffusion mechanism against a considerable concentration gradient. Furthermore, in both Ectocarpus and Macrocystis some boron is most likely bound to cell wall constituent alginate and the photoassimilate mannitol located in sieve cells. Herein, we describe boron uptake, speciation, localization and possible biological function in two species of brown algae, Macrocystis pyrifera and Ectocarpus siliculosus.	[Miller, Eric P.; Carrano, Carl J.] San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA; [Wu, Youxian] San Diego State Univ, Dept Civil Construct & Environm Engn, San Diego, CA 92182 USA	Carrano, CJ (corresponding author), San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA.	ccarrano@mail.sdsu.edu		Miller, Eric/0000-0003-4388-6000	California State University Council on Ocean Affairs, Science and Technology (COAST) Grant Development Award [GDP-2013-002]; California State University Council on Ocean Affairs, Science and Technology (COAST)	This research was supported by a California State University Council on Ocean Affairs, Science and Technology (COAST) Grant Development Award #GDP-2013-002. The authors are grateful to Dr Steve Barlow and the SDSU Electron Microscope Facility for expert technical assistance and instrument time. EM has been supported by a California State University Council on Ocean Affairs, Science and Technology (COAST) graduate scholarship.	ACREE TE, 1973, ADV CHEM SER, P208; Andersen R, 2005, ALGAL CULTURING TECH; Bishop M, 2004, DALTON T, P2621, DOI 10.1039/b406952h; Carrano CJ, 2009, MAR BIOTECHNOL, V11, P431, DOI 10.1007/s10126-009-9191-4; Chuda Y, 1997, PHYTOCHEMISTRY, V46, P209, DOI 10.1016/S0031-9422(97)00284-7; Frommer WB, 2002, NATURE, V420, P282, DOI 10.1038/420282a; GLUSENKAMP KH, 1997, BORON SOILS PLANTS, P229; Goldbach H. E., 2001, BORON PLANT ANIMAL N; HAUG A, 1976, ACTA CHEM SCAND B, V30, P562, DOI 10.3891/acta.chem.scand.30b-0562; Henkel R., 1952, KIELER MEERESFORSCH, V8, P192; Kato Y, 2009, PLANT CELL PHYSIOL, V50, P58, DOI 10.1093/pcp/pcn168; Kaya A, 2009, MOL CELL BIOL, V29, P3665, DOI 10.1128/MCB.01646-08; KNOECK J, 1969, ANAL CHEM, V41, P1730, DOI 10.1021/ac60282a005; Kobayashi M, 1996, PLANT PHYSIOL, V110, P1017, DOI 10.1104/pp.110.3.1017; Konotchick T, 2013, NEW PHYTOL, V198, P398, DOI 10.1111/nph.12160; LEWIN J, 1966, J PHYCOL, V2, P160, DOI 10.1111/j.1529-8817.1966.tb04616.x; LEWIN J, 1976, J EXP BOT, V27, P916, DOI 10.1093/jxb/27.5.916; LEWIN JC, 1966, J EXP BOT, V17, P473, DOI 10.1093/jxb/17.3.473; LEWIN JC, 1965, NATURWISSENSCHAFTEN, V52, P70, DOI 10.1007/BF00695678; MANLEY SL, 1984, J PHYCOL, V20, P192, DOI 10.1111/j.0022-3646.1984.00192.x; MANLEY SL, 1983, J PHYCOL, V19, P118, DOI 10.1111/j.0022-3646.1983.00118.x; MCHUGH DJ, 1987, PRODUCTION UTILIZATI, P58; MCLACHLAN J, 1977, PHYCOLOGIA, V16, P329, DOI 10.2216/i0031-8884-16-3-329.1; MOTOMURA T, 1984, PHYCOLOGIA, V23, P331, DOI 10.2216/i0031-8884-23-3-331.1; Neame D., 1972, ELEMENTARY KINETICS; PARKER BC, 1965, J PHYCOL, V1, P41, DOI 10.1111/j.1529-8817.1965.tb04554.x; RAVEN JA, 1980, NEW PHYTOL, V84, P231, DOI 10.1111/j.1469-8137.1980.tb04424.x; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; Schmid T, 2008, ANAL BIOANAL CHEM, V391, P1907, DOI 10.1007/s00216-008-2101-1; SCHMITZ K, 1979, PLANT PHYSIOL, V63, P995, DOI 10.1104/pp.63.6.995; SCHMITZ K, 1976, MAR BIOL, V36, P207, DOI 10.1007/BF00389281; Spector W. S., 1956, HDB BIOL DATA; SPIVACK AJ, 1993, NATURE, V363, P149, DOI 10.1038/363149a0; Takano J, 2002, NATURE, V420, P337, DOI 10.1038/nature01139; Takano J, 2006, PLANT CELL, V18, P1498, DOI 10.1105/tpc.106.041640; Tanaka M, 2008, PLANT CELL, V20, P2860, DOI 10.1105/tpc.108.058628; Tanaka M, 2008, PFLUG ARCH EUR J PHY, V456, P671, DOI 10.1007/s00424-007-0370-8; VANDENBERG R, 1994, CARBOHYD RES, V253, P1, DOI 10.1016/0008-6215(94)80050-2; Warington K, 1923, ANN BOT-LONDON, V37, P629, DOI 10.1093/oxfordjournals.aob.a089871; YAU SK, 1995, EUPHYTICA, V83, P185, DOI 10.1007/BF01678128	40	7	7	1	18	ROYAL SOC CHEMISTRY	CAMBRIDGE	THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND	1756-5901	1756-591X		METALLOMICS	Metallomics		2016	8	2					161	169		10.1039/c5mt00238a			9	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	DE9PV	WOS:000370971200003	26679972	Other Gold			2021-04-07	
J	Kinoshita, N; Fu, G; Ito, T; Motomura, T				Kinoshita, Nana; Fu, Gang; Ito, Toshiaki; Motomura, Taizo			Three-dimensional organization of flagellar basal apparatus in Ectocarpus gametes	PHYCOLOGICAL RESEARCH			English	Article						anterior flagellum; brown algae; Ectocarpus gametes; flagellar basal apparatus; posterior flagellum	ELECTRON-MICROSCOPE OBSERVATIONS; INTERNAL STRUCTURE; SILICULOSUS ECTOCARPALES; FINE-STRUCTURE; PHAEOPHYCEAE; SCYTOSIPHONALES; ASYMMETRY; ZOOSPORES; CENTRIN; AXIS	The flagellar basal apparatus of the brown alga Ectocarpus siliculosus was re-investigated in details using transmission electron microscopy and electron tomography. As a result, three-dimensional structures with spatial arrangement of bands and microtubular flagellar rootlets were observed. Fibrous structures linking the anterior flagellar basal body to the major anterior rootlet (R3) or the bypassing rootlet was newly discovered in this study. A direct attachment from the minor anterior rootlet (R4) to the anterior and posterior basal bodies was also discovered, as were attachments from the minor posterior rootlet (R1) to the deltoid striated band and from the major posterior rootlet (R2) to the posterior fibrous band. The microtubular flagellar rootlets were connected to the bands and to the anterior or posterior basal body. These bands may have a role in maintaining the spatial arrangement of the anterior and posterior flagellar basal bodies and the microtubular flagellar rootlets. A numbering system of the basal body triplets was established by tracing axonemal doublets in the serial sections. From these observations, the precise position of two flagellar basal bodies, bands, and flagellar rootlets was determined.	[Kinoshita, Nana] Hokkaido Univ, Grad Sch Environm Sci, Sapporo, Hokkaido, Japan; [Ito, Toshiaki] Hokkaido Univ, Res Fac Agr, Electron Microscope Lab, Sapporo, Hokkaido, Japan; [Fu, Gang; Motomura, Taizo] Hokkaido Univ, Field Sci Ctr Nothern Biosphere, Muroran Marine Stn, Muroran, Hokkaido, Japan	Motomura, T (corresponding author), Hokkaido Univ, Field Sci Ctr Nothern Biosphere, Muroran Marine Stn, Muroran, Hokkaido, Japan.	motomura@fsc.hokudai.ac.jp					AFZELIUS B, 1959, J BIOPHYS BIOCHEM CY, V5, P269, DOI 10.1083/jcb.5.2.269; Bui KH, 2009, J CELL BIOL, V186, P437, DOI 10.1083/jcb.200903082; CHEIGNON M, 1964, CR HEBD ACAD SCI, V258, P676; Fu G, 2013, PROTOPLASMA, V250, P261, DOI 10.1007/s00709-012-0405-7; GELLER A, 1981, J EXP BIOL, V92, P53; HENRY EC, 1982, J PHYCOL, V18, P570; HENRY EC, 1982, J PHYCOL, V18, P550; HOOPS HJ, 1983, J CELL BIOL, V97, P902, DOI 10.1083/jcb.97.3.902; KATSAROS CI, 1993, J PHYCOL, V29, P787, DOI 10.1111/j.0022-3646.1993.00787.x; Kawai H., 2000, FLAGELLATES UNITY DI, P124; Kawai H., 1992, KOREAN J PHYCOLOGY, V7, P33; Kinoshita N, 2016, EUR J PHYCOL, V51, P139, DOI 10.1080/09670262.2015.1109144; KREIMER G, 1994, INT REV CYTOL, V148, P229, DOI 10.1016/S0074-7696(08)62409-2; Kremer JR, 1996, J STRUCT BIOL, V116, P71, DOI 10.1006/jsbi.1996.0013; Lin JF, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0046494; MAIER I, 1990, J EXP BOT, V41, P869, DOI 10.1093/jxb/41.7.869; Maier I, 1997, EUR J PHYCOL, V32, P255; Maier I, 1997, EUR J PHYCOL, V32, P241; MANTON I, 1959, J EXP BOT, V10, P448, DOI 10.1093/jxb/10.3.448; MANTON I, 1957, J EXP BOT, V8, P294, DOI 10.1093/jxb/8.2.294; MANTON I, 1950, NATURE, V166, P973, DOI 10.1038/166973a0; MANTON I, 1956, J EXP BOT, V7, P416, DOI 10.1093/jxb/7.3.416; MANTON I, 1951, J EXP BOT, V2, P242, DOI 10.1093/jxb/2.2.242; MANTON I., 1964, NEW PHYTQL, V63, P244, DOI 10.1111/j.1469-8137.1964.tb07377.x; Manton I., 1956, CELLULAR MECHANISMS, P61; Mastronarde DN, 1997, J STRUCT BIOL, V120, P343, DOI 10.1006/jsbi.1997.3919; Matsunaga S, 2010, PHOTOCHEM PHOTOBIOL, V86, P374, DOI 10.1111/j.1751-1097.2009.00676.x; Melkonian M., 1984, SYSTEMATICS GREEN AL, V27, P73; Moestrup O, 2000, SYST ASSOC SPEC VOL, V59, P69; MOTOMURA T, 1989, Japanese Journal of Phycology, V37, P105; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; Nagasato C, 2004, CELL MOTIL CYTOSKEL, V59, P109, DOI 10.1002/cm.20021; Nagasato C, 2002, PROTOPLASMA, V219, P140, DOI 10.1007/s007090200015; OKELLY CJ, 1989, SYST ASSOC SPEC VOL, V38, P255; OKELLY CJ, 1984, PROTOPLASMA, V123, P18, DOI 10.1007/BF01283178; Provasoli L, 1968, CULTURES COLLECTIONS, P63; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; SALE WS, 1986, J CELL BIOL, V102, P2042, DOI 10.1083/jcb.102.6.2042; SALISBURY JL, 1984, J CELL BIOL, V99, P962, DOI 10.1083/jcb.99.3.962; SALISBURY JL, 1988, J CELL BIOL, V107, P635, DOI 10.1083/jcb.107.2.635; Ueki C, 2008, PHYCOLOGIA, V47, P5, DOI 10.2216/0031-8884(2008)47[5:ROTPPA]2.0.CO;2; Woolley DM, 2010, BIOL REV, V85, P453, DOI 10.1111/j.1469-185X.2009.00110.x	42	1	1	0	7	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	1322-0829	1440-1835		PHYCOL RES	Phycol. Res.	JAN	2016	64	1					19	25		10.1111/pre.12115			7	Marine & Freshwater Biology	Marine & Freshwater Biology	DC6CR	WOS:000369307900003		Green Accepted			2021-04-07	
J	Dittami, SM; Duboscq-Bidot, L; Perennou, M; Gobet, A; Corre, E; Boyen, C; Tonon, T				Dittami, Simon M.; Duboscq-Bidot, Laetitia; Perennou, Morgan; Gobet, Angelique; Corre, Erwan; Boyen, Catherine; Tonon, Thierry			Host-microbe interactions as a driver of acclimation to salinity gradients in brown algal cultures	ISME JOURNAL			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; VARIABILITY; ADAPTATION; EVOLUTION; REVEALS; NETWORK; GENOME; GENUS; WATER	Like most eukaryotes, brown algae live in association with bacterial communities that frequently have beneficial effects on their development. Ectocarpus is a genus of small filamentous brown algae, which comprises a strain that has recently colonized freshwater, a rare transition in this lineage. We generated an inventory of bacteria in Ectocarpus cultures and examined the effect they have on acclimation to an environmental change, that is, the transition from seawater to freshwater medium. Our results demonstrate that Ectocarpus depends on bacteria for this transition: cultures that have been deprived of their associated microbiome do not survive a transfer to freshwater, but restoring their microflora also restores the capacity to acclimate to this change. Furthermore, the transition between the two culture media strongly affects the bacterial community composition. Examining a range of other closely related algal strains, we observed that the presence of two bacterial operational taxonomic units correlated significantly with an increase in low salinity tolerance of the algal culture. Despite differences in the community composition, no indications were found for functional differences in the bacterial metagenomes predicted to be associated with algae in the salinities tested, suggesting functional redundancy in the associated bacterial community. Our study provides an example of how microbial communities may impact the acclimation and physiological response of algae to different environments, and thus possibly act as facilitators of speciation. It paves the way for functional examinations of the underlying host-microbe interactions, both in controlled laboratory and natural conditions.	[Dittami, Simon M.; Gobet, Angelique; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, Sorbonne Univ, CNRS, Integrat Biol Marine Models UMR8227,Stn Biol Rosc, Pl Georges Teissier, F-28688 Roscoff, France; [Duboscq-Bidot, Laetitia] Univ Nantes, UMR 1087, Inst Rech Therapeut, Plateforme Genom, Nantes, France; [Perennou, Morgan] UPMC, CNRS, Plateforme Sequencage Genotypage, FR 2424,Stn Biol, Roscoff, France; [Corre, Erwan] UPMC, CNRS, FR 2424, ABiMS Platform,Stn Biol, Roscoff, France	Dittami, SM (corresponding author), Univ Paris 06, Sorbonne Univ, CNRS, Integrat Biol Marine Models UMR8227,Stn Biol Rosc, Pl Georges Teissier, F-28688 Roscoff, France.	simon.dittami@sb-roscoff.fr	corre, erwan/O-4669-2019; Tonon, Thierry/A-3214-2009; Gobet, Angelique/B-7572-2013; Dittami, Simon/E-8354-2011	corre, erwan/0000-0001-6354-2278; Tonon, Thierry/0000-0002-1454-6018; Gobet, Angelique/0000-0003-4204-8451; Dittami, Simon/0000-0001-7987-7523	French Government via the National Research Agency investment expenditure program IDEALG [ANR-10-BTBR-02-04]; region Brittany via the SAD (Strategie d'Attractivite Durable) project COGEBRAL	We are grateful to Catherine Leblanc, Christian Jeanthon, Dominique Davoult, Philippe Potin, Elianne Sirnaes Egge and Tristan Barbeyron for helpful advice on the experimental setup and analysis. We also thank Ludovic Delage for providing Zobellia galactanivorans DNA, Akira Peters (BEZHIN ROSKO) for providing algal strains, Declan Schroeder for allowing us to use the two South African algal strains, Laurence Dartevelle for performing antibiotic treatments on strain 371, Jonas Collen for critical reading of the manuscript, and the Biogenouest Genomics and Genomer platform core facility for technical support. This work benefited from the support of the French Government via the National Research Agency investment expenditure program IDEALG (ANR-10-BTBR-02-04) and additional support came from the region Brittany via the SAD (Strategie d'Attractivite Durable) project COGEBRAL.	BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; Billoud B, 2014, NUCLEIC ACIDS RES, V42, P417, DOI 10.1093/nar/gkt856; Bold H. C., 1985, INTRO ALGAE STRUCTUR; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Burke C, 2011, ISME J, V5, P590, DOI 10.1038/ismej.2010.164; Caporaso JG, 2012, ISME J, V6, P1621, DOI 10.1038/ismej.2012.8; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chisholm JRM, 1996, NATURE, V381, P382, DOI 10.1038/381382a0; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coulthurst SJ, 2013, RES MICROBIOL, V164, P640, DOI 10.1016/j.resmic.2013.03.017; Croft MT, 2006, EUKARYOT CELL, V5, P1175, DOI 10.1128/EC.00097-06; Deligianni E, 2010, BMC MICROBIOL, V10, DOI 10.1186/1471-2180-10-38; Dittami SM, 2014, FRONT GENET, V5, DOI 10.3389/fgene.2014.00241; Dittami SM, 2014, MOL ECOL, V23, P1656, DOI 10.1111/mec.12670; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Egan S, 2013, FEMS MICROBIOL REV, V37, P462, DOI 10.1111/1574-6976.12011; Goecke F, 2010, MAR ECOL PROG SER, V409, P267, DOI 10.3354/meps08607; Guttenplan SB, 2013, FEMS MICROBIOL REV, V37, P849, DOI 10.1111/1574-6976.12018; Hammer Oyvind, 2001, Palaeontologia Electronica, V4, pUnpaginated; Hollants J, 2013, FEMS MICROBIOL ECOL, V83, P1, DOI 10.1111/j.1574-6941.2012.01446.x; Hubbell Stephen P., 2001, V32, pi; Langille MGI, 2013, NAT BIOTECHNOL, V31, P814, DOI 10.1038/nbt.2676; Lee CE, 1999, TRENDS ECOL EVOL, V14, P284, DOI 10.1016/S0169-5347(99)01596-7; Logares R, 2009, TRENDS MICROBIOL, V17, P414, DOI 10.1016/j.tim.2009.05.010; Magurran AE., 2004, MEASURING BIOL DIVER; Maignien L, 2014, MBIO, V5, DOI 10.1128/mBio.00682-13; Meslet-Cladiere L, 2013, PLANT CELL, V25, P3089, DOI 10.1105/tpc.113.111336; Oksanen J., 2013, PACKAGE VEGAN COMMUN, P1; Parks DH, 2010, BIOINFORMATICS, V26, P715, DOI 10.1093/bioinformatics/btq041; PEDERSEN M, 1968, NATURE, V218, P776, DOI 10.1038/218776a0; PEDERSEN M, 1973, PHYSIOL PLANTARUM, V28, P101, DOI 10.1111/j.1399-3054.1973.tb01158.x; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peterson AT, 2011, ECOLOGICAL NICHES GE; Pommier T, 2007, MOL ECOL, V16, P867, DOI 10.1111/j.1365-294X.2006.03189.x; R Core Team, 2014, R LANG ENV STAT COMP; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; SALE PF, 1979, OECOLOGIA, V42, P159, DOI 10.1007/BF00344855; Schloss PD, 2009, APPL ENVIRON MICROB, V75, P7537, DOI 10.1128/AEM.01541-09; Smoot ME, 2011, BIOINFORMATICS, V27, P431, DOI 10.1093/bioinformatics/btq675; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Staufenberger T, 2008, FEMS MICROBIOL ECOL, V64, P65, DOI 10.1111/j.1574-6941.2008.00445.x; Tatarenkov A, 2005, MOL ECOL, V14, P647, DOI 10.1111/j.1365-294X.2005.02425.x; Tonon T, 2011, OMICS, V15, P883, DOI 10.1089/omi.2011.0089; Torode TA, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0118366; Wahl M, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00292; West John A., 1996, Muelleria, V9, P29; Zilber-Rosenberg I, 2008, FEMS MICROBIOL REV, V32, P723, DOI 10.1111/j.1574-6976.2008.00123.x; Zinger L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0024570; Zobell CE, 1941, J MAR RES, V4, P42	52	36	37	1	48	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1751-7362	1751-7370		ISME J	ISME J.	JAN	2016	10	1					51	63		10.1038/ismej.2015.104			13	Ecology; Microbiology	Environmental Sciences & Ecology; Microbiology	CY8PR	WOS:000366671300007	26114888	Green Published, Bronze			2021-04-07	
J	Petursdottir, AH; Fletcher, K; Gunnlaugsdottir, H; Krupp, E; Kupper, FC; Feldmann, J				Petursdottir, Asta H.; Fletcher, Kyle; Gunnlaugsdottir, Helga; Krupp, Eva; Kuepper, Frithjof C.; Feldmann, Joerg			Environmental effects on arsenosugars and arsenolipids in Ectocarpus (Phaeophyta)	ENVIRONMENTAL CHEMISTRY			English	Article						chloroplasts; cultures; HPLC-ESIMS; HPLC-ICP-MS; lipid-soluble arsenic; speciation	ARSENIC-CONTAINING HYDROCARBONS; CANNED COD-LIVER; BROWN ALGA; FISH OILS; RP-HPLC; IDENTIFICATION; PHOSPHATE; PHYTOPLANKTON; ACCUMULATION; SILICULOSUS	Environmental context Arsenolipids, which are present in seaweed, can show high toxicity, emphasising the need for more information on these compounds. We investigated the effects of different stress factors on the arsenic compounds formed by cultures of brown algae, and compared the results with those from field-collected samples. We show that the arsenolipid and arsenosugar profiles differ depending on the experimental conditions, and that a deficiency in phosphate has a direct positive effect on the biosynthesis of arsenic-containing phospholipids. Abstract Seaweeds have recently been shown to contain a significant proportion of arsenic in the form of arsenolipids (AsLp). Three strains of the filamentous brown alga Ectocarpus species were grown in the laboratory with different simulations of environmental stress: control conditions (1/2 Provasoli-enriched seawater), low nitrate (30% of the amount of nitrates in the control), low phosphate (30% of the amount of phosphate in the control) and under oxidative stress levels (2mM H2O2). Generally, the major AsLp was an arsenic-containing hydrocarbon, AsHC360 (50-80%), but additionally, several arsenic-containing phospholipids (AsPL) were identified and quantified using high-performance liquid chromatography-inductively coupled plasma mass spectrometry and electrospray ionisation mass spectrometry (HPLC-ICP-MS/ESI-MS). The AsLps in cultures were compared with AsLps in Ectocarpus found in its natural habitat as well as with other brown filamentous algae. The AsLp and arsenosugar profiles differed depending on the experimental conditions. Under low phosphate conditions, a significant reduction of phosphorus-containing arsenosugars was noticed, and a significant increase of phosphate-containing AsLps was found when compared with the controls. Strains grown under oxidative stress showed a significant increase in AsLps as well as clear physiological changes.	[Petursdottir, Asta H.; Krupp, Eva; Feldmann, Joerg] Univ Aberdeen, Dept Chem, TESLA, Aberdeen AB24 3UE, Scotland; [Petursdottir, Asta H.; Fletcher, Kyle; Kuepper, Frithjof C.] Univ Aberdeen, Oceanlab, Newburgh AB41 6AA, Scotland; [Petursdottir, Asta H.; Gunnlaugsdottir, Helga] Matis, Food Safety Environm & Genet Dept, IS-113 Reykjavik, Iceland	Petursdottir, AH (corresponding author), Univ Aberdeen, Dept Chem, TESLA, Aberdeen AB24 3UE, Scotland.	astap@matis.is; j.feldmann@abdn.ac.uk	Feldmann, Jorg/AAT-3703-2020	Gunnlaugsdottir, Helga/0000-0002-1883-6936; Kuepper, Frithjof/0000-0003-1273-7109; Petursdottir, Asta/0000-0002-6739-0598; Feldmann, Joerg/0000-0002-0524-8254	Icelandic research fund [130542-051]; SORSAS award; College of Physical Sciences at Aberdeen University; MASTS(The Marine Alliance for Science and Technology for Scotland) pooling initiative; Scottish Funding Council [HR09011]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [1093492] Funding Source: researchfish	We thank Gillian Milne, of the Aberdeen Microscopy Facility, University of Aberdeen, for help in preparing and viewing the samples through TEM and Ingo Maier, from the Universitat Konstanz, for kindly providing us with an optimised processing schedule for the fixation of Ectocarpus for TEM. We also express our gratitude to Dawn Shewring for her help with algal culturing. A.H. Petursdottir thanks the Icelandic research fund (grant reference 130542-051), the SORSAS award and The College of Physical Sciences at Aberdeen University for financial support. F. C. Kupper also received funding from the MASTS(The Marine Alliance for Science and Technology for Scotland) pooling initiative and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions.	Alexander J, 2009, EFSA J, V7, DOI 10.2903/j.efsa.2009.1351; Almela C, 2006, FOOD CHEM TOXICOL, V44, P1901, DOI 10.1016/j.fct.2006.06.011; Amayo KO, 2014, TALANTA, V118, P217, DOI 10.1016/j.talanta.2013.09.056; Amayo KO, 2013, ANAL CHEM, V85, P9321, DOI 10.1021/ac4020935; Amayo KO, 2011, ANAL CHEM, V83, P3589, DOI 10.1021/ac2005873; [Anonymous], 2010, NSWFACP0431102; Arroyo-Abad U, 2014, EUR J LIPID SCI TECH, V116, P1381, DOI 10.1002/ejlt.201400144; Arroyo-Abad U, 2013, FOOD CHEM, V141, P3093, DOI 10.1016/j.foodchem.2013.05.152; Arroyo-Abad U, 2010, TALANTA, V82, P38, DOI 10.1016/j.talanta.2010.03.054; Bold H. C., 1985, INTRO ALGAE STRUCTUR; Borak J, 2007, REGUL TOXICOL PHARM, V47, P204, DOI 10.1016/j.yrtph.2006.09.005; Castlehouse H, 2003, ENVIRON SCI TECHNOL, V37, P951, DOI 10.1021/es026110i; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; COONEY RV, 1978, P NATL ACAD SCI USA, V75, P4262, DOI 10.1073/pnas.75.9.4262; Diaz O, 2012, FOOD CHEM TOXICOL, V50, P744, DOI 10.1016/j.fct.2011.11.024; Duncan EG, 2013, MAR CHEM, V157, P78, DOI 10.1016/j.marchem.2013.08.004; EDMONDS JS, 1987, EXPERIENTIA, V43, P553, DOI 10.1007/BF02143584; EDMONDS JS, 1993, MAR POLLUT BULL, V26, P665, DOI 10.1016/0025-326X(93)90549-Y; Foster S, 2008, MAR CHEM, V108, P172, DOI 10.1016/j.marchem.2007.11.005; Francesconi KA, 2010, PURE APPL CHEM, V82, P373, DOI 10.1351/PAC-CON-09-07-01; Garcia-Salgado S, 2012, ENVIRON CHEM, V9, P63, DOI 10.1071/EN11164; Glabonjat RA, 2014, ANAL CHEM, V86, P10282, DOI 10.1021/ac502488f; Hellweger FL, 2003, LIMNOL OCEANOGR, V48, P2275; Ruiz-Chancho MJ, 2012, J ANAL ATOM SPECTROM, V27, P501, DOI 10.1039/c1ja10260e; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Lischka S, 2013, TALANTA, V110, P144, DOI 10.1016/j.talanta.2013.02.051; Meyer S, 2014, METALLOMICS, V6, P1023, DOI 10.1039/c4mt00061g; MORITA M, 1988, CHEMOSPHERE, V17, P1147, DOI 10.1016/0045-6535(88)90180-4; Muller DG, 2008, CAH BIOL MAR, V49, P59; Musil S, 2014, ANAL CHEM, V86, P993, DOI 10.1021/ac403438c; Navratilova J, 2011, ENVIRON CHEM, V8, P44, DOI 10.1071/EN10107; Patey MD, 2008, TRAC-TREND ANAL CHEM, V27, P169, DOI 10.1016/j.trac.2007.12.006; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Petursdottir A. H., 2014, THESIS; Raab A, 2013, ANAL CHEM, V85, P2817, DOI 10.1021/ac303340t; Raber G, 2009, TALANTA, V78, P1215, DOI 10.1016/j.talanta.2009.01.013; Rumpler A, 2008, ANGEW CHEM INT EDIT, V47, P2665, DOI 10.1002/anie.200705405; Sele V, 2014, TALANTA, V121, P89, DOI 10.1016/j.talanta.2013.12.049; Sele V, 2013, ANAL BIOANAL CHEM, V405, P5179, DOI 10.1007/s00216-013-6925-y; Sele V, 2012, FOOD CHEM, V133, P618, DOI 10.1016/j.foodchem.2012.02.004; Smedley PL, 2002, APPL GEOCHEM, V17, P517, DOI 10.1016/S0883-2927(02)00018-5; Starr R.C., 1987, Journal of Phycology, V23, P1; Taleshi MS, 2008, CHEM COMMUN, P4706, DOI 10.1039/b808049f; Taleshi MS, 2014, SCI REP-UK, V4, DOI 10.1038/srep07492; Taleshi MS, 2010, ENVIRON SCI TECHNOL, V44, P1478, DOI 10.1021/es9030358; ULLRICHEBERIUS CI, 1989, J EXP BOT, V40, P119, DOI 10.1093/jxb/40.1.119; Van Mooy BAS, 2009, NATURE, V458, P69, DOI 10.1038/nature07659	48	16	16	7	62	CSIRO PUBLISHING	CLAYTON	UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA	1448-2517	1449-8979		ENVIRON CHEM	Environ. Chem.		2016	13	1					21	33		10.1071/EN14229			13	Chemistry, Analytical; Environmental Sciences	Chemistry; Environmental Sciences & Ecology	DA1ZV	WOS:000367596000004		Other Gold			2021-04-07	
J	Godfroy, O; Peters, AF; Coelho, SM; Cock, JM				Godfroy, Olivier; Peters, Akira F.; Coelho, Susana M.; Cock, J. Mark			Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus	MARINE GENOMICS			English	Article						Brown algae; Ectocarpus; Ethyl methanesulphonate; Mutagenesis; Ultraviolet	DNA-SEQUENCING DATA; LIFE-CYCLE; INDUCED MUTATIONS; SILICULOSUS; PHAEOPHYCEAE; ARABIDOPSIS; FRAMEWORK; GENETICS	Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). (C) 2015 Elsevier B.V. All rights reserved.	[Godfroy, Olivier; Coelho, Susana M.; Cock, J. Mark] Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, CNRS,Algal Genet Grp,UMR 8227,Stn Biol Roscoff, F-29688 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France	Cock, JM (corresponding author), Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, CNRS,Algal Genet Grp,UMR 8227,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.		Coelho, Susana/ABH-8166-2020	Cock, J. Mark/0000-0002-2650-0383	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); University Pierre and Marie Curie; Agence Nationale de la Recherche Investment for the Future project IdealgFrench National Research Agency (ANR); European Union Infrastructure project Assemble; CNRSCentre National de la Recherche Scientifique (CNRS)European Commission	This work was supported by the Centre National de la Recherche Scientifique and the University Pierre and Marie Curie. O.G. was supported by the Agence Nationale de la Recherche Investment for the Future project Idealg, the European Union Infrastructure project Assemble and the CNRS.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; BAUTZ E, 1960, P NATL ACAD SCI USA, V46, P1585, DOI 10.1073/pnas.46.12.1585; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P262, DOI 10.1101/pdb.prot067942; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Dahmani-Mardas F, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0015776; Dalmais M, 2008, GENOME BIOL, V9, DOI 10.1186/gb-2008-9-2-r43; DePristo MA, 2011, NAT GENET, V43, P491, DOI 10.1038/ng.806; Flibotte S, 2010, GENETICS, V185, P431, DOI 10.1534/genetics.110.116616; Greene EA, 2003, GENETICS, V164, P731; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hendriks G, 2010, CURR BIOL, V20, P170, DOI 10.1016/j.cub.2009.11.061; Ikehata H, 2011, J RADIAT RES, V52, P115, DOI 10.1269/jrr.10175; KOORNNEEF M, 1982, MUTAT RES, V93, P109, DOI 10.1016/0027-5107(82)90129-4; Kurowska M, 2011, J APPL GENET, V52, P371, DOI 10.1007/s13353-011-0061-1; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Li H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324; McCallum CM, 2000, NAT BIOTECHNOL, V18, P455; McKenna A, 2010, GENOME RES, V20, P1297, DOI 10.1101/gr.107524.110; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; Muller DG, 1975, LYNGB ARCH PROTISTEN, V117, P297; Ossowski S, 2008, GENOME RES, V18, P2024, DOI 10.1101/gr.080200.108; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Schmieder R, 2011, BIOINFORMATICS, V27, P863, DOI 10.1093/bioinformatics/btr026	31	8	9	1	13	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	1874-7787	1876-7478		MAR GENOM	Mar. Genom.	DEC	2015	24	1	1		SI		109	113		10.1016/j.margen.2015.03.007			5	Genetics & Heredity; Marine & Freshwater Biology	Genetics & Heredity; Marine & Freshwater Biology	DA4MV	WOS:000367774900013	25861732				2021-04-07	
J	Fletcher, K; Zuljevic, A; Tsirigoti, A; Antolic, B; Katsaros, C; Nikolic, V; van West, P; Kupper, FC				Fletcher, Kyle; Zuljevic, Ante; Tsirigoti, Amerssa; Antolic, Boris; Katsaros, Christos; Nikolic, Vedran; van West, Pieter; Kuepper, Frithjof C.			New record and phylogenetic affinities of the oomycete Olpidiopsis feldmanni infecting Asparagopsis sp (Rhodophyta)	DISEASES OF AQUATIC ORGANISMS			English	Article						Central vacuole; Cyochrome oxidase subunit II; COII; cox2; SSU rRNA; Falkenbergia; Transmission electron microscopy; TEM; Adriatic Sea	EURYCHASMA-DICKSONII; MOLECULAR PHYLOGENY; ECTOCARPUS-SILICULOSUS; EUKARYOTIC PATHOGENS; PYLAIELLA-LITTORALIS; RED ALGAE; SP-NOV; PARASITE; ULTRASTRUCTURE; SUSCEPTIBILITY	A new geographic record of the oomycete Olpidiopsis feldmanni infecting the tetrasporophytic stage of the red alga Asparagopsis sp. from the Adriatic Sea, confirmed through morphological identification, allowed us to expand previous observations of this organism. Ultrastructural investigations of environmental material showed a large central vacuole and a cell wall thicker than previously reported from other basal oomycete pathogens of algae. Phylogenetic analysis closely associates O. feldmanni to O. bostrychiae concurrent with structural observations. This constitutes the first genetic characterisation of an Olpidiopsis species that was initially described before 1960, adding to the genetic data of 3 other marine Olpidiopsis species established and genetically characterised in the last 2 decades. The paper discusses concurrences of the ultrastructural observations made here and in previous studies of the marine Olpidiopsis species with those made on the freshwater species.	[Fletcher, Kyle; Kuepper, Frithjof C.] Univ Aberdeen, Oceanlab, Newburgh AB41 6AA, England; [Fletcher, Kyle; van West, Pieter] Univ Aberdeen, Aberdeen Oomycete Lab, Aberdeen AB25 2ZD, Scotland; [Zuljevic, Ante; Antolic, Boris; Nikolic, Vedran] Inst Oceanog & Fisheries, Lab Benthos, HR-21000 Split, Croatia; [Tsirigoti, Amerssa; Katsaros, Christos] Univ Athens, Dept Bot, Fac Biol, Athens 15784, Greece	Kupper, FC (corresponding author), Univ Aberdeen, Oceanlab, Newburgh AB41 6AA, England.	fkuepper@abdn.ac.uk	zuljevic, ante/R-7455-2016	van West, Pieter/0000-0002-0767-6017; Kuepper, Frithjof/0000-0003-1273-7109; TSIRIGOTI, AMERSSA/0000-0002-5018-3461	Total Foundation (Paris); UK Natural Environment Research Council (NERC)UK Research & Innovation (UKRI)NERC Natural Environment Research Council; MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland - Scottish Funding Council) [HR09011]; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [BB/J018333/1] Funding Source: researchfish; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/F012578/1, 1093492] Funding Source: researchfish	We are grateful to the Total Foundation (Paris) for its funding support to this study, to the UK Natural Environment Research Council (NERC) for a doctoral fellowship to K.F. and to the MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011).	ALEEM A. A., 1952, Compte Rendu de l'Academie des Sciences, V235, P1250; Aleem AA, 1953, ARK BOT, V3, P1; ANDREWS JH, 1976, BIOL REV, V51, P211, DOI 10.1111/j.1469-185X.1976.tb01125.x; BORTNICK RN, 1985, MYCOLOGIA, V77, P861, DOI 10.2307/3793298; Dick MW., 2001, STRAMINIPILOUS FUNGI; Dixon P.S., 1977, SEAWEEDS BRIT ISLES; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Garzoli L, 2014, MAR ECOL, V36, P959, DOI DOI 10.1111/MAEC.12189; Hall TA.., 1999, NUCL ACIDS S SERIES, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Hudspeth DSS, 2000, MYCOLOGIA, V92, P674, DOI 10.2307/3761425; Jokl M, 1916, OESTERR BOT Z, V66, P267; Karling JS, 1943, AM J BOT, V30, P637, DOI 10.2307/2437479; KARLING JS, 1949, MYCOLOGIA, V41, P270, DOI 10.2307/3755107; Karling JS, 1942, SIMPLE HOLOCARPIC BI, V1; Klochkova TA, 2016, J APPL PHYCOL, V28, P73, DOI 10.1007/s10811-015-0595-4; Klochkova TA, 2012, J APPL PHYCOL, V24, P135, DOI 10.1007/s10811-011-9661-8; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; KUMAR CR, 1980, CAN J BOT, V58, P2557, DOI 10.1139/b80-298; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; LANGERSAFER PR, 1982, P NATL ACAD SCI-BIOL, V79, P4381, DOI 10.1073/pnas.79.14.4381; Lin YC, 2012, APPL ENVIRON MICROB, V78, P3387, DOI 10.1128/AEM.06952-11; Macey BM, 2011, AQUACULTURE, V315, P187, DOI 10.1016/j.aquaculture.2011.02.004; MARTIN RW, 1986, MYCOLOGIA, V78, P230, DOI 10.2307/3793168; MARTIN RW, 1986, MYCOLOGIA, V78, P359, DOI 10.2307/3793039; Massana R, 2004, APPL ENVIRON MICROB, V70, P3528, DOI 10.1128/AEM.70.6.3528-3534.2004; Phillips Naomi, 2001, Phycological Research, V49, P97, DOI 10.1111/j.1440-1835.2001.tb00239.x; PUESCHEL CM, 1985, CAN J BOT, V63, P409, DOI 10.1139/b85-049; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Sekimoto S, 2008, MYCOL RES, V112, P361, DOI 10.1016/j.mycres.2007.11.002; Sekimoto S, 2009, PHYCOLOGIA, V48, P460, DOI 10.2216/08-11.1; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES; Stamatakis A, 2006, BIOINFORMATICS, V22, P2688, DOI 10.1093/bioinformatics/btl446; Stoeck T, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000728; Strittmatter M, 2013, DIS AQUAT ORGAN, V104, P1, DOI 10.3354/dao02583; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tsirigoti A, 2014, PLANT BIOLOGY, V16, P272, DOI 10.1111/plb.12041; Tsirigoti A, 2015, PROTOPLASMA, V252, P845, DOI 10.1007/s00709-014-0721-1; West JA, 2006, PHYCOL RES, V54, P72, DOI 10.1111/j.1440-1835.2006.00410.x; WHITTICK A, 1972, ARCH MIKROBIOL, V82, P353, DOI 10.1007/BF00424938	40	7	11	0	3	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0177-5103	1616-1580		DIS AQUAT ORGAN	Dis. Aquat. Org.	NOV 17	2015	117	1					45	57		10.3354/dao02930			13	Fisheries; Veterinary Sciences	Fisheries; Veterinary Sciences	CX1QC	WOS:000365469700005	26575155	Bronze			2021-04-07	
J	Luthringer, R; Lipinska, AP; Roze, D; Cormier, A; Macaisne, N; Peters, AF; Cock, JM; Coelho, SM				Luthringer, Remy; Lipinska, Agnieszka P.; Roze, Denis; Cormier, Alexandre; Macaisne, Nicolas; Peters, Akira F.; Cock, J. Mark; Coelho, Susana M.			The Pseudoautosomal Regions of the U/V Sex Chromosomes of the Brown Alga Ectocarpus Exhibit Unusual Features	MOLECULAR BIOLOGY AND EVOLUTION			English	Article						pseudoautosomal region; sex chromosomes; UV sexual system; brown algae	BIASED GENE-EXPRESSION; CODON USAGE; EVOLUTIONARY DYNAMICS; GENOME; RECOMBINATION; DROSOPHILA; SEQUENCE; SELECTION; RATES; MAP	The recombining regions of sex chromosomes (pseudoautosomal regions, PARs) are predicted to exhibit unusual features due to their being genetically linked to the nonrecombining, sex-determining region. This phenomenon is expected to occur in both diploid (XY, ZW) and haploid (UV) sexual systems, with slightly different consequences for UV sexual systems because of the absence of masking during the haploid phase (when sex is expressed) and because there is no homozygous sex in these systems. Despite a considerable amount of theoretical work on PAR genetics and evolution, these genomic regions have remained poorly characterized empirically. We show here that although the PARs of the U/V sex chromosomes of the brown alga Ectocarpus recombine at a similar rate to autosomal regions of the genome, they exhibit many genomic features typical of nonrecombining regions. The PARs were enriched in clusters of genes that are preferentially, and often exclusively, expressed during the sporophyte generation of the life cycle, and many of these genes appear to have evolved since the Ectocarpales diverged from other brown algal lineages. A modeling-based approach was used to investigate possible evolutionary mechanisms underlying this enrichment in sporophyte-biased genes. Our results are consistent with the evolution of the PAR in haploid systems being influenced by differential selection pressures in males and females acting on alleles that are advantageous during the sporophyte generation of the life cycle.	[Luthringer, Remy; Lipinska, Agnieszka P.; Cormier, Alexandre; Macaisne, Nicolas; Cock, J. Mark; Coelho, Susana M.] Univ Paris 06, Sorbonne Univ, UMR 8227,Stn Biol Roscoff, Algal Genet Grp,Integrat Biol Marine Models,CNRS, Roscoff, France; [Roze, Denis] Univ Paris 06, Sorbonne Univ, Evolutionary Biol & Ecol Algae,CNRS, UMI 3614,PUCCh,UACH,Stn Biol Roscoff, Roscoff, France	Coelho, SM (corresponding author), Univ Paris 06, Sorbonne Univ, UMR 8227,Stn Biol Roscoff, Algal Genet Grp,Integrat Biol Marine Models,CNRS, Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020	Cock, J. Mark/0000-0002-2650-0383; Cormier, Alexandre/0000-0002-7775-8413; Macaisne, Nicolas/0000-0002-0109-9845	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [ANR12-JSV7-0008, ANR-10-BLAN-1727]; University Pierre; Marie Curie Emergence program; Interreg program France (Channel)-England (project MARINEXUS); ERCEuropean Research Council (ERC)European Commission [638240]	This work was supported by the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (project SEXSEAWEED ANR12-JSV7-0008, project Bi-CYCLE ANR-10-BLAN-1727, and project IDEALG), the University Pierre and Marie Curie Emergence program, the Interreg program France (Channel)-England (project MARINEXUS), and the ERC (grant agreement 638240). The authors thank Lynn Delgat and Anne Vanderheyden for help with the alignments for the dN/dS analysis, Claire Gachon for sharing unpublished sequence data from E. fasciculatus and E. sp. lineage 1c Greenland, and Didier Jollivet and Thomas Broquet for helpful discussions. The authors also like to thank three anonymous reviewers for their comments and suggestions that helped improve the manuscript.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Anders S, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-10-r106; Arendsee ZW, 2014, TRENDS PLANT SCI, V19, P698, DOI 10.1016/j.tplants.2014.07.003; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Bachtrog D, 2011, TRENDS GENET, V27, P350, DOI 10.1016/j.tig.2011.05.005; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; BURGOYNE PS, 1992, CELL, V71, P391, DOI 10.1016/0092-8674(92)90509-B; Burt DW, 2002, CYTOGENET GENOME RES, V96, P97, DOI 10.1159/000063018; Campos JL, 2012, GENOME BIOL EVOL, V4, P278, DOI 10.1093/gbe/evs010; Charlesworth B, 2014, EVOLUTION, V68, P1339, DOI 10.1111/evo.12364; Charlesworth D, 2005, HEREDITY, V95, P118, DOI 10.1038/sj.hdy.6800697; Charlesworth D, 2005, CURR BIOL, V15, pR129, DOI 10.1016/j.cub.2005.02.011; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Couceiro L, 2015, EVOLUTION, V69, P1808, DOI 10.1111/evo.12702; Criscione CD, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r71; Duret L, 2000, MOL BIOL EVOL, V17, P68, DOI 10.1093/oxfordjournals.molbev.a026239; Duret L, 1999, P NATL ACAD SCI USA, V96, P4482, DOI 10.1073/pnas.96.8.4482; Flaquer A, 2008, EUR J HUM GENET, V16, P771, DOI 10.1038/ejhg.2008.63; Gobler CJ, 2011, P NATL ACAD SCI USA, V108, P4352, DOI 10.1073/pnas.1016106108; Haddrill PR, 2007, GENOME BIOL, V8, DOI 10.1186/gb-2007-8-2-r18; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hillier LW, 2004, NATURE, V432, P695, DOI 10.1038/nature03154; Hinch AG, 2014, PLOS GENET, V10, DOI 10.1371/journal.pgen.1004503; Hsueh YP, 2006, PLOS GENET, V2, P1702, DOI 10.1371/journal.pgen.0020184; Immler S, 2015, EVOLUTION; Innocenti P, 2010, PLOS BIOL, V8, DOI 10.1371/journal.pbio.1000335; Janes DE, 2009, J HERED, V100, P125, DOI 10.1093/jhered/esn065; Jensen-Seaman MI, 2004, GENOME RES, V14, P528, DOI 10.1101/gr.1970304; Kanaya S, 2001, J MOL EVOL, V53, P290, DOI 10.1007/s002390010219; Kirkpatrick M, 2014, GENETICS, V197, P531, DOI 10.1534/genetics.113.156026; Kondo M, 2001, GENET RES, V78, P23, DOI 10.1017/S0016672301005109; Lamour KH, 2012, MOL PLANT MICROBE IN, V25, P1350, DOI 10.1094/MPMI-02-12-0028-R; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; LEWIS K. R., 1960, PHYTON REV INTERNAC BOT EXPTL ARGENTINA, V14, P21; Lewis K. R., 1968, International Review of Cytology, V23, P277, DOI 10.1016/S0074-7696(08)60274-0; Lewis KR., 1961, T BRIT BRYOL SOC, V4, P111, DOI DOI 10.1179/006813861804870514; Lien S, 2000, AM J HUM GENET, V66, P557, DOI 10.1086/302754; Lipinska A, 2015, MOL BIOL EVOL, V32, P1581, DOI 10.1093/molbev/msv049; Montoya-Burgos JI, 2003, TRENDS GENET, V19, P128, DOI 10.1016/S0168-9525(03)00021-0; Mugford ST, 2013, PLANT CELL, V25, P1078, DOI 10.1105/tpc.113.110551; Otto SP, 2011, TRENDS GENET, V27, P358, DOI 10.1016/j.tig.2011.05.001; PAGE D C, 1987, Genomics, V1, P243, DOI 10.1016/0888-7543(87)90051-6; Palmieri N, 2014, ELIFE, V3, DOI 10.7554/eLife.01311; Parsch J, 2013, NAT REV GENET, V14, P83, DOI 10.1038/nrg3376; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Puigbo P, 2008, BIOL DIRECT, V3, DOI 10.1186/1745-6150-3-38; Radakovits R, 2012, NAT COMMUN, V3, DOI 10.1038/ncomms1688; Raudsepp T, 2012, SEX DEV, V6, P72, DOI 10.1159/000330627; ROUYER F, 1986, NATURE, V319, P291, DOI 10.1038/319291a0; Shi QH, 2001, AM J MED GENET, V99, P34, DOI 10.1002/1096-8628(20010215)99:1<34::AID-AJMG1106>3.0.CO;2-D; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Smeds L, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms6448; SORIANO P, 1987, P NATL ACAD SCI USA, V84, P7218, DOI 10.1073/pnas.84.20.7218; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Swanson WJ, 2004, GENETICS, V168, P1457, DOI 10.1534/genetics.104.030478; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tautz D, 2011, NAT REV GENET, V12, P692, DOI 10.1038/nrg3053; Vicoso B, 2006, NAT REV GENET, V7, P645, DOI 10.1038/nrg1914; Vicoso B, 2013, P NATL ACAD SCI USA, V110, P6453, DOI 10.1073/pnas.1217027110; Vieler A, 2012, PLOS GENET, V8, DOI 10.1371/journal.pgen.1003064; Wai CM, 2012, CHROMOSOME RES, V20, P753, DOI 10.1007/s10577-012-9312-1; Williams EJB, 2002, J MOL EVOL, V54, P511, DOI 10.1007/s00239-001-0043-8; Wu XM, 2013, GENOME BIOL EVOL, V5, P1731, DOI 10.1093/gbe/evt115; Ye NH, 2015, NAT COMMUN, V6, DOI 10.1038/ncomms7986; Yin T, 2008, GENOME RES, V18, P422, DOI 10.1101/gr.7076308	67	13	13	0	35	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0737-4038	1537-1719		MOL BIOL EVOL	Mol. Biol. Evol.	NOV	2015	32	11					2973	2985		10.1093/molbev/msv173			13	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	CT7YZ	WOS:000363033100014	26248564	Green Accepted, Bronze, Green Published			2021-04-07	
J	Lipinska, AP; Ahmed, S; Peters, AF; Faugeron, S; Cock, JM; Coelho, SM				Lipinska, Agnieszka P.; Ahmed, Sophia; Peters, Akira F.; Faugeron, Sylvain; Cock, J. Mark; Coelho, Susana M.			Development of PCR-Based Markers to Determine the Sex of Kelps	PLOS ONE			English	Article							BROWN ALGA ECTOCARPUS; UNDARIA-PINNATIFIDA; MACROCYSTIS-PYRIFERA; FEMALE GAMETOPHYTES; SILICULOSUS PHAEOPHYCEAE; JAPONICA PHAEOPHYCEAE; SACCHARINA-JAPONICA; TRIAL CULTIVATION; GENOME SEQUENCE; YELLOW CATFISH	Sex discriminating genetic markers are commonly used to facilitate breeding programs in economically and ecologically important animal and plant species. However, despite their considerable economic and ecological value, the development of sex markers for kelp species has been very limited. In this study, we used the recently described sequence of the sex determining region (SDR) of the brown algal model Ectocarpus to develop novel DNA-based sex-markers for three commercially relevant kelps: Laminaria digitata, Undaria pinnatifida and Macrocystis pyrifera. Markers were designed within nine protein coding genes of Ectocarpus male and female (U/V) sex chromosomes and tested on gametophytes of the three kelp species. Seven primer pairs corresponding to three loci in the Ectocarpus SDR amplified sex-specific bands in the three kelp species, yielding at least one male and one female marker for each species. Our work has generated the first male sex-specific markers for L. digitata and U. pinnatifida, as well as the first sex markers developed for the genus Macrocystis. The markers and methodology presented here will not only facilitate seaweed breeding programs but also represent useful tools for population and demography studies and provide a means to investigate the evolution of sex determination across this largely understudied eukaryotic group.	[Lipinska, Agnieszka P.; Ahmed, Sophia; Cock, J. Mark; Coelho, Susana M.] Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, Algal Genet Grp,Stn Biol Roscoff,UMR 8227,CS 9007, F-29688 Roscoff, France; [Lipinska, Agnieszka P.; Ahmed, Sophia; Cock, J. Mark; Coelho, Susana M.] CNRS, Integrat Biol Marine Models, Stn Biol Roscoff, Algal Genet Grp,UMR 8227,CS 90074, F-29688 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France; [Faugeron, Sylvain] Univ Paris 06, Sorbonne Univ, Evolutionary Biol & Ecol Algae, Stn Biol Roscoff,UMI 3614,CS 90074, F-29688 Roscoff, France; [Faugeron, Sylvain] CNRS, Evolutionary Biol & Ecol Algae, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; [Faugeron, Sylvain] Pontificia Univ Catolica Chile, Ctr Conservac Marina, Santiago, Chile; [Faugeron, Sylvain] Pontificia Univ Catolica Chile, Fac Ciencias Biol, CeBiB, Santiago, Chile	Coelho, SM (corresponding author), Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, Algal Genet Grp,Stn Biol Roscoff,UMR 8227,CS 9007, F-29688 Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020	Faugeron, Sylvain/0000-0001-7258-5229; Peters, Akira/0000-0001-5332-199X; Cock, J. Mark/0000-0002-2650-0383	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [ANR12-JSV7-0008, ANR-10-BLAN-1727]; University Pierre and Marie Curie Emergence program; Interreg program France (Channel)-England (project MARINEXUS); Bezhin Rosko	This work was supported by the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (Project SEXSEAWEED ANR12-JSV7-0008, project Bi-CYCLE ANR-10-BLAN-1727 and project IDEALG), the University Pierre and Marie Curie Emergence program, and the Interreg program France (Channel)-England (project MARINEXUS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Bezhin Rosko provided support in the form of salaries for authors [AFP], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Bold H. C., 1985, INTRO ALGAE; Carney LT, 2010, J PHYCOL, V46, P987, DOI 10.1111/j.1529-8817.2010.00882.x; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, PLANT SIGNAL BEHAV, V6, P1858, DOI 10.4161/psb.6.11.17737; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Correa T, 2016, AQUAC RES, V47, P698, DOI 10.1111/are.12529; Critchley AT, 1993, JPN INT COOP AGENCY, P151; Dan C, 2013, INT J BIOL SCI, V9, P1043, DOI 10.7150/ijbs.7203; Danilova TV, 2006, EUPHYTICA, V151, P15, DOI 10.1007/s10681-005-9020-4; DAYTON PK, 1985, ANNU REV ECOL SYST, V16, P215, DOI 10.1146/annurev.es.16.110185.001243; Dayton PK, 1998, ECOL APPL, V8, P309, DOI 10.2307/2641070; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; DRUEHL LD, 1989, MAR BIOL, V101, P451, DOI 10.1007/BF00541646; Duarte CM, 2009, BIOSCIENCE, V59, P967, DOI 10.1525/bio.2009.59.11.8; Ellegren H, 1996, P ROY SOC B-BIOL SCI, V263, P1635, DOI 10.1098/rspb.1996.0239; Fang Z. X., 1980, ACTA GENET SIN, V7, P19; Graham MH, 2007, OCEANOGR MAR BIOL, V45, P39; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Gu JG, 2014, J APPL PHYCOL, V26, P635, DOI 10.1007/s10811-013-0089-1; Guillemin ML, 2012, J PHYCOL, V48, P365, DOI 10.1111/j.1529-8817.2012.01116.x; Guiry M. D., 2015, ALGAEBASE; Gutierrez A, 2006, 18 INT SEAW S, P33, DOI DOI 10.1007/978-1-4020-5670.3-5; HASEGAWA Y, 1976, J FISH RES BOARD CAN, V33, P1002, DOI 10.1139/f76-127; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Henry EC, 1987, PHYCOLOGIA, V387, P393; HORMAZA JI, 1994, THEOR APPL GENET, V89, P9, DOI 10.1007/BF00226975; Hughes AD, 2012, BIOTECHNOL BIOFUELS, V5, DOI 10.1186/1754-6834-5-86; Ince AG, 2011, J AGR SCI-CAMBRIDGE, V149, P327, DOI 10.1017/S0021859610000948; Kawai H, 2013, SCI REP-UK, V3, DOI 10.1038/srep02491; Kijjoa A., 2004, MAR DRUGS, V2, P73, DOI [DOI 10.3390/MD202073, 10.3390/md202073]; Kim S-K, 2011, HANDBOOK OF MARINE M; Koressaar T, 2007, BIOINFORMATICS, V23, P1289, DOI 10.1093/bioinformatics/btm091; Kovacs B, 2000, GENETICA, V110, P267, DOI 10.1023/A:1012739318941; Lane CE, 2006, J PHYCOL, V42, P493, DOI 10.1111/j.1529-8817.2006.00204.x; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; LEWIS RJ, 1993, J PHYCOL, V29, P363, DOI 10.1111/j.0022-3646.1993.00363.x; Li XJ, 2008, AQUACULTURE, V280, P76, DOI 10.1016/j.aquaculture.2008.05.005; Li XJ, 2007, J APPL PHYCOL, V19, P139, DOI 10.1007/s10811-006-9120-0; Liao LQ, 2009, EUPHYTICA, V169, P49, DOI 10.1007/s10681-009-9913-8; Lipinska A, 2015, MOL BIOL EVOL, DOI [10.1093/molbev/msv049, DOI 10.1093/M0LBEV/MSV049]; Lipinska AP, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-909; Liu YS, 2009, J PHYCOL, V45, P894, DOI 10.1111/j.1529-8817.2009.00719.x; Liu Y, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0048784; Luthringer R, 2014, PERSPECT PHYCOL, V1, P11, DOI DOI 10.1127/2198-011X/2014/0002; Luthringer R, 2015, MOL BIOL EVOL, V32, P2973, DOI 10.1093/molbev/msv173; Macchiavello J, 2010, J APPL PHYCOL, V22, P691, DOI 10.1007/s10811-010-9508-8; MANN KH, 1973, SCIENCE, V182, P975, DOI 10.1126/science.182.4116.975; MARKHAM J W, 1972, Phycologia, V11, P147, DOI 10.2216/i0031-8884-11-2-147.1; Martinez EA, 1999, MOL ECOL, V8, P1533, DOI 10.1046/j.1365-294x.1999.00721.x; Morelissen B, 2013, J EXP MAR BIOL ECOL, V448, P197, DOI 10.1016/j.jembe.2013.07.009; Oppliger LV, 2007, J PHYCOL, V43, P1295, DOI 10.1111/j.1529-8817.2007.00408.x; Oppliger LV, 2011, J PHYCOL, V47, P5, DOI 10.1111/j.1529-8817.2010.00930.x; Parasnis AS, 2000, MOL BREEDING, V6, P337, DOI 10.1023/A:1009678807507; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Reamon-Buttner SM, 2000, THEOR APPL GENET, V100, P432, DOI 10.1007/s001220050056; Sauvageau C, 1918, PARIS MEM ACAD SCI; Shan TF, 2013, PHYCOL RES, V61, P154, DOI 10.1111/pre.12014; Shan TF, 2010, PHYCOL RES, V58, P171, DOI 10.1111/j.1440-1835.2010.00575.x; Shan TF, 2009, PHYCOL RES, V57, P36, DOI 10.1111/j.1440-1835.2008.00519.x; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Smale DA, 2013, ECOL EVOL, V3, P4016, DOI 10.1002/ece3.774; Smit AJ, 2004, J APPL PHYCOL, V16, P245, DOI 10.1023/B:JAPH.0000047783.36600.ef; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Untergasser A, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gks596; Oppliger LV, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0102518; Wang D, 2009, ANIM GENET, V40, P978, DOI 10.1111/j.1365-2052.2009.01941.x; Wang WJ, 2013, PLANTA, V237, P1123, DOI 10.1007/s00425-012-1831-7; Westermeier R, 2006, AQUAC RES, V37, P164, DOI 10.1111/j.1365-2109.2005.01414.x; Westermeier R, 2007, J APPL PHYCOL, V19, P215, DOI 10.1007/s10811-006-9126-7; Westermeier R, 2010, J APPL PHYCOL, V22, P357, DOI 10.1007/s10811-009-9466-1; Wu C-Y, 1996, CHIN J OCEANOL LIMN, V14, P205, DOI [10.1007/BfF02850381, DOI 10.1007/BF02850381]; YABU H, 1973, Bulletin of the Faculty of Fisheries Hokkaido University, V23, P171; YONESHIGUEVALENTIN Y, 1990, HYDROBIOLOGIA, V204, P461, DOI 10.1007/BF00040271; Zhang N, 2015, BMC GENOMICS, V16, DOI 10.1186/s12864-015-1371-1	82	18	19	0	41	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	OCT 23	2015	10	10							e0140535	10.1371/journal.pone.0140535			15	Multidisciplinary Sciences	Science & Technology - Other Topics	CU1VG	WOS:000363309200018	26496392	DOAJ Gold, Green Published			2021-04-07	
J	Zhang, L; Wang, XM; Liu, T; Wang, HY; Wang, GL; Chi, S; Liu, C				Zhang, Lei; Wang, Xumin; Liu, Tao; Wang, Haiyang; Wang, Guoliang; Chi, Shan; Liu, Cui			Complete Plastid Genome of the Brown Alga Costaria costata (Laminariales, Phaeophyceae)	PLOS ONE			English	Article							RNA GENES; SEQUENCE; MITOCHONDRIAL; CHLOROPLAST; RATES; POLYSACCHARIDES; GENERATION; PHYLOGENY	Costaria costata is a commercially and industrially important brown alga. In this study, we used next-generation sequencing to determine the complete plastid genome of C. costata. The genome consists of a 129,947 bp circular DNA molecule with an A+T content of 69.13% encoding a standard set of six ribosomal RNA genes, 27 transfer RNA genes, and 137 protein-coding genes with two conserved open reading frames (ORFs). The overall genome structure of C. costata is nearly the same as those of Saccharina japonica and Undaria pinnatifida. The plastid genomes of these three algal species retain a strong conservation of the GTG start codon while infrequently using TGA as a stop codon. In this regard, they differ substantially from the plastid genomes of Ectocarpus siliculosus and Fucus vesiculosus. Analysis of the nucleic acid substitution rates of the Laminariales plastid genes revealed that the petF gene has the highest substitution rate and the petN gene contains no substitution over its complete length. The variation in plastid genes between C. costata and S. japonica is lower than that between C. costata and U. pinnatifida as well as that between U. pinnatifida and S. japonica. Phylogenetic analyses demonstrated that C. costata and U. pinnatifida have a closer genetic relationship. We also identified two gene length mutations caused by the insertion or deletion of repeated sequences, which suggest a mechanism of gene length mutation that may be one of the key explanations for the genetic variation in plastid genomes.	[Zhang, Lei; Liu, Tao; Wang, Haiyang; Chi, Shan; Liu, Cui] Ocean Univ China, Coll Marine Life Sci, Lab Genet & Breeding Marine Organism, Qingdao, Peoples R China; [Wang, Xumin; Wang, Guoliang] Chinese Acad Sci, Beijing Inst Genom, Beijing Key Lab Genome & Precis Med Technol, CAS Key Lab Genome Sci & Informat, Beijing, Peoples R China	Liu, T (corresponding author), Ocean Univ China, Coll Marine Life Sci, Lab Genet & Breeding Marine Organism, Qingdao, Peoples R China.	liutao@ouc.edu.cn			Special Fund for Agro-scientific Research in the Public Interest [201203063]; Project of Shandong Province Science and Technology Development Program [2014GGE29091]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [201362040]	This work was supported by the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 201203063), Project of Shandong Province Science and Technology Development Program (Grant No. 2014GGE29091) and the Fundamental Research Funds for the Central Universities (Grant No. 201362040).	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Archibald JM, 2012, ADV BOT RES, V64, P87, DOI 10.1016/B978-0-12-391499-6.00003-7; Boo Sung Min, 1999, Phycological Research, V47, P109, DOI 10.1111/j.1440-1835.1999.tb00291.x; BOYEN C, 1994, NUCLEIC ACIDS RES, V22, P1400, DOI 10.1093/nar/22.8.1400; CLEGG MT, 1994, P NATL ACAD SCI USA, V91, P6795, DOI 10.1073/pnas.91.15.6795; Doyle JJ., 1990, FOCUS, V12, P39; Druehl LD, 1997, PLANT SYST EVOL, V11, P35; Ellegren H, 2000, TRENDS GENET, V16, P551, DOI 10.1016/S0168-9525(00)02139-9; [付慧 Fu Hui], 2012, [大连海洋大学学报, Journal of Dalian Ocean University], V27, P200; Imbs TI, 2011, CHEM NAT COMPD+, V47, P96, DOI 10.1007/s10600-011-9839-y; Imbs TI, 2009, CHEM NAT COMPD+, V45, P786, DOI 10.1007/s10600-010-9507-7; Kearse M, 2012, BIOINFORMATICS, V28, P1647, DOI 10.1093/bioinformatics/bts199; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; LEVINSON G, 1987, MOL BIOL EVOL, V4, P203; Li TY, 2014, MITOCHONDR DNA, P1; Lohse M, 2007, CURR GENET, V52, P267, DOI 10.1007/s00294-007-0161-y; Lowe TM, 1997, NUCLEIC ACIDS RES, V25, P955, DOI 10.1093/nar/25.5.955; Luo RB, 2012, GIGASCIENCE, V1, DOI 10.1186/2047-217X-1-18; Luo XM, 2012, BIOCHEM SYST ECOL, V44, P61, DOI 10.1016/j.bse.2012.04.004; Moon HJ, 2009, J NAT PROD, V72, P1731, DOI 10.1021/np800797v; PEABODY DS, 1989, J BIOL CHEM, V264, P5031; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; Qu JQ, 2014, MITOCHONDR DNA, P1; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; Ronquist F, 2003, BIOINFORMATICS, V19, P1572, DOI 10.1093/bioinformatics/btg180; SAUNDERS GW, 1992, J PHYCOL, V28, P544, DOI 10.1111/j.0022-3646.1992.00544.x; Selivanova ON, 2007, RUSS J MAR BIOL +, V33, P278, DOI 10.1134/S1063074007050021; Smith DR, 2015, GENOME BIOL EVOL, V7, P1227, DOI 10.1093/gbe/evv069; Sohn CH, 2003, J PHYCOL, P53; Tajima N, 2014, J PLANT RES, V127, P389, DOI 10.1007/s10265-014-0627-1; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Wang L, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0065902; Wang XL, 2013, MAR GENOM, V10, P1, DOI 10.1016/j.margen.2012.12.002; WOLFE KH, 1987, P NATL ACAD SCI USA, V84, P9054, DOI 10.1073/pnas.84.24.9054; Yoon HS, 2001, MOL PHYLOGENET EVOL, V21, P231, DOI 10.1006/mpev.2001.1009; Zhang Jing, 2011, Journal of Ocean University of China, V10, P351, DOI 10.1007/s11802-011-1856-8; Zhang ZY, 1992, J DALIAN FISHERIES U, V7, P40	37	13	14	1	18	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	OCT 7	2015	10	10							e0140144	10.1371/journal.pone.0140144			12	Multidisciplinary Sciences	Science & Technology - Other Topics	CT0TS	WOS:000362510600144	26444909	DOAJ Gold, Green Published			2021-04-07	
J	Zhang, L; Wang, XM; Liu, T; Wang, GL; Chi, S; Liu, C; Wang, HY				Zhang, Lei; Wang, Xumin; Liu, Tao; Wang, Guoliang; Chi, Shan; Liu, Cui; Wang, Haiyang			Complete Plastid Genome Sequence of the Brown Alga Undaria pinnatifida	PLOS ONE			English	Article							CHLOROPLAST GENOME; ORIGINS; ORGANIZATION; GENERATION; EVOLUTION; SEAWEED; PROTEIN	In this study, we fully sequenced the circular plastid genome of a brown alga, Undaria pinnatifida. The genome is 130,383 base pairs (bp) in size; it contains a large single-copy (LSC, 76,598 bp) and a small single-copy region (SSC, 42,977 bp), separated by two inverted repeats (IRa and IRb: 5,404 bp). The genome contains 139 protein-coding, 28 tRNA, and 6 rRNA genes; none of these genes contains introns. Organization and gene contents of the U. pinnatifida plastid genome were similar to those of Saccharina japonica. There is a co-linear relationship between the plastid genome of U. pinnatifida and that of three previously sequenced large brown algal species. Phylogenetic analyses of 43 taxa based on 23 plastid protein-coding genes grouped all plastids into a red or green lineage. In the large brown algae branch, U. pinnatifida and S. japonica formed a sister clade with much closer relationship to Ectocarpus siliculosus than to Fucus vesiculosus. For the first time, the start codon ATT was identified in the plastid genome of large brown algae, in the atpA gene of U. pinnatifida. In addition, we found a gene-length change induced by a 3-bp repetitive DNA in ycf35 and ilvB genes of the U. pinnatifida plastid genome.	[Zhang, Lei; Liu, Tao; Chi, Shan; Liu, Cui; Wang, Haiyang] Ocean Univ China, Coll Marine Life Sci, Lab Genet & Breeding Marine Organism, Qingdao, Peoples R China; [Wang, Xumin; Wang, Guoliang] Chinese Acad Sci, Beijing Inst Genom, Beijing Key Lab Genome & Precis Med Technol, CAS Key Lab Genome Sci & Informat, Beijing, Peoples R China	Liu, T (corresponding author), Ocean Univ China, Coll Marine Life Sci, Lab Genet & Breeding Marine Organism, Qingdao, Peoples R China.	liutao@ouc.edu.cn			Special Fund for Agro-scientific Research in the Public Interest [201203063]; Project of Shandong Province Science and Technology Development Program [2014GGE29091]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [201362040]	This work was supported by the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 201203063), Project of Shandong Province Science and Technology Development Program (Grant No. 2014GGE29091) and the Fundamental Research Funds for the Central Universities (Grant No. 201362040).	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; BHATTACHARYA D, 1995, J PHYCOL, V31, P489, DOI 10.1111/j.1529-8817.1995.tb02542.x; Cavalier-Smith T, 1999, J EUKARYOT MICROBIOL, V46, P347, DOI 10.1111/j.1550-7408.1999.tb04614.x; Doyle JJ., 1990, FOCUS, V12, P39; Dreyer Hermann, 2006, Frontiers in Zoology, V3, P1; Green BR, 2011, PHOTOSYNTH RES, V107, P103, DOI 10.1007/s11120-010-9584-2; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; HOELZEL AR, 1991, MOL BIOL EVOL, V8, P475; Kearse M, 2012, BIOINFORMATICS, V28, P1647, DOI 10.1093/bioinformatics/bts199; Khan H, 2007, MOL BIOL EVOL, V24, P1832, DOI 10.1093/molbev/msm101; Kim E., 2009, V13, P1, DOI 10.1007/7089_2008_17; Kimura J, 2002, J NAT PROD, V65, P57, DOI 10.1021/np0103057; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; Lemieux C, 2000, NATURE, V403, P649, DOI 10.1038/35001059; Li TY, 2014, MITOCHONDR DNA, P1; Lohse M, 2007, CURR GENET, V52, P267, DOI 10.1007/s00294-007-0161-y; Lowe TM, 1997, NUCLEIC ACIDS RES, V25, P955, DOI 10.1093/nar/25.5.955; Luo RB, 2012, GIGASCIENCE, V1, DOI 10.1186/2047-217X-1-18; Ohta N, 2003, DNA RES, V10, P67, DOI 10.1093/dnares/10.2.67; PEABODY DS, 1989, J BIOL CHEM, V264, P5031; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; Pronicki M, 2008, J CLIN PATHOL, V61, P460, DOI 10.1136/jcp.2007.051060; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; Rogers MB, 2007, MOL BIOL EVOL, V24, P54, DOI 10.1093/molbev/msl129; Ronquist F, 2003, BIOINFORMATICS, V19, P1572, DOI 10.1093/bioinformatics/btg180; Sanchez-Puerta MV, 2007, MOL PHYLOGENET EVOL, V44, P885, DOI 10.1016/j.ympev.2007.03.003; SHINOZAKI K, 1986, EMBO J, V5, P2043, DOI 10.1002/j.1460-2075.1986.tb04464.x; Silva Paul C., 2002, Biological Invasions, V4, P333, DOI 10.1023/A:1020991726710; Synytsya A, 2010, CARBOHYD POLYM, V81, P41, DOI 10.1016/j.carbpol.2010.01.052; Tajima N, 2014, J PLANT RES, V127, P389, DOI 10.1007/s10265-014-0627-1; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Wang L, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0065902; Wang XL, 2013, MAR GENOM, V10, P1, DOI 10.1016/j.margen.2012.12.002; Wyman SK, 2004, BIOINFORMATICS, V20, P3252, DOI 10.1093/bioinformatics/bth352; Yoon HS, 2002, J PHYCOL, V38, P40, DOI DOI 10.1046/j.1529-8817.38.s1.7.x; Zhang L, 2012, J ENG FIBER FABR, V7, P7	36	8	9	0	17	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	OCT 1	2015	10	10							e0139366	10.1371/journal.pone.0139366			12	Multidisciplinary Sciences	Science & Technology - Other Topics	CS6GT	WOS:000362177100052	26426800	DOAJ Gold, Green Published			2021-04-07	
J	Bonin, P; Groisillier, A; Raimbault, A; Guibert, A; Boyen, C; Tonon, T				Bonin, Patricia; Groisillier, Agnes; Raimbault, Alice; Guibert, Anais; Boyen, Catherine; Tonon, Thierry			Molecular and biochemical characterization of mannitol-1-phosphate dehydrogenase from the model brown alga Ectocarpus sp.	PHYTOCHEMISTRY			English	Article						Brown algae; Ectocarpus sp.; Mannitol cycle; Mannitol-1-phosphate dehydrogenase; Recombinant protein	MANNITOL METABOLISM; D-MANNITOL-1-PHOSPHATE DEHYDROGENASE; EVOLUTION; PHAEOPHYCEAE; INSIGHTS; CYCLE; RESOURCE; CLONING; BINDING; GENOME	The sugar alcohol mannitol is important in the food, pharmaceutical, medical and chemical industries. It is one of the most commonly occurring polyols in nature, with the exception of Archaea and animals. It has a range of physiological roles, including as carbon storage, compatible solute, and osmolyte. Mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-l-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22). Analysis of the model brown alga Ectocarpus sp. genome provided three candidate genes for M1PDH activities. We report here the sequence analysis of Ectocarpus M1PDH5 (E5M1PDH5), and the biochemical characterization of the recombinant catalytic domain of EsM1PDH1 (E5M1PDH1cat). Ectocarpus M1PDH5 are representatives of a new type of modular M1PDH5 among the polyol-specific long-chain dehydrogenases/reductases (PSLDRs). The N-terminal domain of EsM1PDH1 was not necessary for enzymatic activity. Determination of kinetic parameters indicated that EsM1PDH1cat displayed higher catalytic efficiency for F6P reduction compared to M1P oxidation. Both activities were influenced by NaCl concentration and inhibited by the thioreactive compound pHMB. These observations were completed by measurement of endogenous M1PDH activity and of EsM1PDH gene expression during one diurnal cycle. No significant changes in enzyme activity were monitored between day and night, although transcription of two out of three genes was altered, suggesting different levels of regulation for this key metabolic pathway in brown algal physiology. (C) 2015 Elsevier Ltd. All rights reserved.	[Bonin, Patricia; Groisillier, Agnes; Raimbault, Alice; Guibert, Anais; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, Sorbonne Univ, Stn Biol Roscoff, CNRS,UMR 8227,Integrat Biol Marine Models, F-29688 Roscoff, France	Groisillier, A (corresponding author), Univ Paris 06, Sorbonne Univ, Stn Biol Roscoff, CNRS,UMR 8227,Integrat Biol Marine Models, CS 90074, F-29688 Roscoff, France.	patricia.bonin@outlook.com; groisill@sb-roscoff.fr; alice.raimbault@orange.fr; anais.guibert02@gmail.com; boyen@sb-roscoff.fr; tonon@sb-roscoff.fr	Patricia, Bonin/AAA-4462-2021; Tonon, Thierry/A-3214-2009	Patricia, Bonin/0000-0002-4194-7343; Tonon, Thierry/0000-0002-1454-6018	IDEALG "Investissements d'avenir, Biotechnologies-Bioresources"French National Research Agency (ANR) [ANR-10-BTBR-02]	The authors thank Dr. Fanny Gaillard and Karine Cahier for mass spectrometry analyses within the METABOMER facilities, Sylvie Rousvoal and Dr. Simon M. Dittami for providing E. siliculosus diurnal samples, Marie-Pascale Prud'homme, Remi Lemoine and Didier Flament for fruitful discussions. Patricia Bonin received a PhD Grant by the Emergence-UPMC 2011 research program. This work also benefited from the support of the project IDEALG (ANR-10-BTBR-02) "Investissements d'avenir, Biotechnologies-Bioresources".	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Aitken A, 1996, TRENDS CELL BIOL, V6, P341, DOI 10.1016/0962-8924(96)10029-5; Allocco JJ, 2001, J PARASITOL, V87, P1441, DOI 10.1645/0022-3395(2001)087[1441:NMBETD]2.0.CO;2; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Banik U, 1997, J BIOL CHEM, V272, P26219, DOI 10.1074/jbc.272.42.26219; Bellamacina CR, 1996, FASEB J, V10, P1257; Bhatt Sheelendra Mangal, 2013, ISRN Biotechnol, V2013, P914187, DOI 10.5402/2013/914187; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; CHASE T, 1986, BIOCHEM J, V239, P435, DOI 10.1042/bj2390435; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Deng YY, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0039704; Dittami SM, 2011, PLANT SIGNAL BEHAV, V6, P1237, DOI 10.4161/psb.6.8.16404; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Eggert A, 2006, EUR J PHYCOL, V41, P405, DOI 10.1080/09670260600919831; Enquist-Newman M, 2014, NATURE, V505, P239, DOI 10.1038/nature12771; Gasteiger E, 2005, PROTEOMICS PROTOCOLS, P571, DOI [10.1385/1-59259-584-7:531, DOI 10.1385/1-59259-890-0:571]; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Groisillier A, 2010, MICROB CELL FACT, V9, DOI 10.1186/1475-2859-9-45; Groisillier A, 2014, J EXP BOT, V65, P559, DOI 10.1093/jxb/ert405; Hall TA.., 1999, NUCL ACIDS S SERIES, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Heinrich S, 2012, EUR J PHYCOL, V47, P83, DOI 10.1080/09670262.2012.660639; IKAWA T, 1972, PLANT CELL PHYSIOL, V13, P1017; Iwamoto K, 2005, MAR BIOTECHNOL, V7, P407, DOI 10.1007/s10126-005-0029-4; Iwamoto K, 2003, PLANT PHYSIOL, V133, P893, DOI 10.1104/pp.103.026906; Karsten U, 1997, PLANTA, V201, P173, DOI 10.1007/BF01007701; KISER RC, 1981, ARCH BIOCHEM BIOPHYS, V211, P613, DOI 10.1016/0003-9861(81)90496-3; Klimacek M, 2003, CHEM-BIOL INTERACT, V143, P559, DOI 10.1016/S0009-2797(02)00219-3; Konotchick T, 2013, NEW PHYTOL, V198, P398, DOI 10.1111/nph.12160; Krahulec S, 2008, CARBOHYD RES, V343, P1414, DOI 10.1016/j.carres.2008.04.011; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; McCauley LAR, 2007, PHYCOLOGIA, V46, P429, DOI 10.2216/05-08.1; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; NOVOTNY MJ, 1984, J BACTERIOL, V159, P986, DOI 10.1128/JB.159.3.986-990.1984; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Pichersky E, 2011, ANNU REV PLANT BIOL, V62, P549, DOI 10.1146/annurev-arplant-042110-103814; Prigent S, 2014, PLANT J, V80, P367, DOI 10.1111/tpj.12627; Rambhatla P, 2011, J MICROBIOL BIOTECHN, V21, P914, DOI 10.4014/jmb.1104.04020; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; RICHTER DFE, 1987, PLANTA, V170, P528, DOI 10.1007/BF00402987; Robert X, 2014, NUCLEIC ACIDS RES, V42, pW320, DOI 10.1093/nar/gku316; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Rousvoal S, 2011, PLANTA, V233, P261, DOI 10.1007/s00425-010-1295-6; Saha BC, 2011, APPL MICROBIOL BIOT, V89, P879, DOI 10.1007/s00253-010-2979-3; Sand M., 2014, PARASITOLOGY; Sand M, 2013, ENVIRON MICROBIOL, V15, P2187, DOI 10.1111/1462-2920.12090; Schmatz DM, 1997, PARASITOLOGY, V114, pS81; SCHMATZ DM, 1989, MOL BIOCHEM PARASIT, V32, P263, DOI 10.1016/0166-6851(89)90075-3; Shao ZR, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0097935; Solomon PS, 2007, TRENDS MICROBIOL, V15, P257, DOI 10.1016/j.tim.2007.04.002; Stiller JW, 2014, NAT COMMUN, V5, DOI 10.1038/ncomms6764; Stoop JMH, 1996, TRENDS PLANT SCI, V1, P139, DOI 10.1016/S1360-1385(96)80048-3; Studier FW, 2005, PROTEIN EXPRES PURIF, V41, P207, DOI 10.1016/j.pep.2005.01.016; Suvarna K, 2000, MICROBIOL-SGM, V146, P2705, DOI 10.1099/00221287-146-10-2705; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tenhaken R, 2011, J BIOL CHEM, V286, P16707, DOI 10.1074/jbc.M111.230979; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; van Hal JW, 2014, TRENDS BIOTECHNOL, V32, P231, DOI 10.1016/j.tibtech.2014.02.007; Wang ZL, 2010, CARBOHYD RES, V345, P50, DOI 10.1016/j.carres.2009.09.020; Wargacki AJ, 2012, SCIENCE, V335, P308, DOI 10.1126/science.1214547; Wei N, 2013, TRENDS BIOTECHNOL, V31, P70, DOI 10.1016/j.tibtech.2012.10.009; Wisselink HW, 2002, INT DAIRY J, V12, P151, DOI 10.1016/S0958-6946(01)00153-4; Wong TKM, 2007, J PHYCOL, V43, P528, DOI 10.1111/j.1529-8817.2007.00349.x	66	8	8	0	24	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND	0031-9422			PHYTOCHEMISTRY	Phytochemistry	SEP	2015	117						509	520		10.1016/j.phytochem.2015.07.015			12	Biochemistry & Molecular Biology; Plant Sciences	Biochemistry & Molecular Biology; Plant Sciences	CR3SL	WOS:000361253300053	26232554				2021-04-07	
J	Tarver, JE; Cormier, A; Pinzon, N; Taylor, RS; Carre, W; Strittmatter, M; Seitz, H; Coelho, SM; Cock, JM				Tarver, James E.; Cormier, Alexandre; Pinzon, Natalia; Taylor, Richard S.; Carre, Wilfrid; Strittmatter, Martina; Seitz, Herve; Coelho, Susana M.; Cock, J. Mark			microRNAs and the evolution of complex multicellularity: identification of a large, diverse complement of microRNAs in the brown alga Ectocarpus	NUCLEIC ACIDS RESEARCH			English	Article							SMALL NONCODING RNAS; DICTYOSTELIUM-DISCOIDEUM; OFFSET RNAS; MIRNAS; GENES; EXPRESSION; ORIGINS; GENOME; HISTORY; ANNOTATION	There is currently convincing evidence that microRNAs have evolved independently in at least six different eukaryotic lineages: animals, land plants, chlorophyte green algae, demosponges, slime molds and brown algae. MicroRNAs from different lineages are not homologous but some structural features are strongly conserved across the eukaryotic tree allowing the application of stringent criteria to identify novel microRNA loci. A large set of 63 microRNA families was identified in the brown alga Ectocarpus based on mapping of RNA-seq data and nine microRNAs were confirmed by northern blotting. The Ectocarpus microRNAs are highly diverse at the sequence level with few multi-gene families, and do not tend to occur in clusters but exhibit some highly conserved structural features such as the presence of a uracil at the first residue. No homologues of Ecto-carpus microRNAs were found in other stramenopile genomes indicating that they emerged late in stramenopile evolution and are perhaps specific to the brown algae. The large number of microRNA loci in Ectocarpus is consistent with the developmental complexity of many brown algal species and supports a proposed link between the emergence and expansion of microRNA regulatory systems and the evolution of complex multicellularity.	[Tarver, James E.; Taylor, Richard S.] Univ Bristol, Sch Earth Sci, Bristol BS8 1TQ, Avon, England; [Tarver, James E.] Natl Univ Ireland, Dept Biol, Genome Evolut Lab, Maynooth, Kildare, Ireland; [Cormier, Alexandre; Carre, Wilfrid; Strittmatter, Martina; Coelho, Susana M.; Cock, J. Mark] Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, CS 90074, F-29688 Roscoff, France; [Pinzon, Natalia; Seitz, Herve] CNRS, UPR 1142, Inst Human Genet, F-34396 Montpellier 5, France	Cock, JM (corresponding author), Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, Algal Genet Grp,UMR 8227,Integrat Biol Marine Mod, CS 90074, F-29688 Roscoff, France.	cock@sb-roscoff.fr	Seitz, Herve/AAE-6487-2019; Pinzon, Natalia/K-5160-2016; Coelho, Susana/ABH-8166-2020	Seitz, Herve/0000-0001-8172-5393; Pinzon, Natalia/0000-0002-0093-2487; Cormier, Alexandre/0000-0002-7775-8413; Cock, J. Mark/0000-0002-2650-0383; Taylor, Richard/0000-0003-1417-715X; Strittmatter, Martina/0000-0002-1258-9751	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); University Pierre and Marie Curie; Interreg program France (Channel) - England (project Marinexus); University Pierre and Marie Curie (Emergence program); Agence National de la Recherche (Future Investment program project Idealg)French National Research Agency (ANR); Irish Research Council EMPOWER Postdoctoral FellowshipIrish Research Council for Science, Engineering and Technology; Agence National de la Recherche (ANR, French National Research Agency)French National Research Agency (ANR); Agence National de la Recherche (Young Investigator project Sexseaweed)French National Research Agency (ANR); Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); University Pierre and Marie Curie; Interreg program France (Channel) - England (project Marinexus); University Pierre and Marie Curie (Emergence program); Agence National de la Recherche (Future Investment program project Idealg)French National Research Agency (ANR); Irish Research Council EMPOWER Postdoctoral FellowshipIrish Research Council for Science, Engineering and Technology; Agence National de la Recherche (ANR, French National Research Agency)French National Research Agency (ANR); Agence National de la Recherche (Young Investigator project Sexseaweed)French National Research Agency (ANR); Engineering and Physical Sciences Research CouncilUK Research & Innovation (UKRI)Engineering & Physical Sciences Research Council (EPSRC) [1098841] Funding Source: researchfish	Centre National de la Recherche Scientifique, the University Pierre and Marie Curie, the Interreg program France (Channel) - England (project Marinexus); University Pierre and Marie Curie (Emergence program); Agence National de la Recherche (Future Investment program project Idealg and Young Investigator project Sexseaweed); Irish Research Council EMPOWER Postdoctoral Fellowship (to J.E.T.). Funding for open access charge: Agence National de la Recherche (ANR, French National Research Agency).	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Allen E, 2004, NAT GENET, V36, P1282, DOI 10.1038/ng1478; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Aryal R, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-20; Avesson L, 2012, RNA, V18, P1771, DOI 10.1261/rna.033175.112; Axtell MJ, 2013, ANNU REV PLANT BIOL, V64, P137, DOI 10.1146/annurev-arplant-050312-120043; Axtell MJ, 2011, GENOME BIOL, V12, DOI 10.1186/gb-2011-12-4-221; Bartel DP, 2009, CELL, V136, P215, DOI 10.1016/j.cell.2009.01.002; Berezikov E, 2011, NAT REV GENET, V12, P846, DOI 10.1038/nrg3079; Berezikov E, 2011, GENOME RES, V21, P203, DOI 10.1101/gr.116657.110; Biasiolo M, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0023854; Billoud B, 2014, NUCLEIC ACIDS RES, V42, P417, DOI 10.1093/nar/gkt856; Bonnet E, 2010, BIOINFORMATICS, V26, P1566, DOI 10.1093/bioinformatics/btq233; Bortoluzzi S, 2011, TRENDS MOL MED, V17, P473, DOI 10.1016/j.molmed.2011.05.005; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Brodersen P, 2008, SCIENCE, V320, P1185, DOI 10.1126/science.1159151; Brown J. W., 2010, PLOS ONE, V5; Campo-Paysaa F, 2011, EVOL DEV, V13, P15, DOI 10.1111/j.1525-142X.2010.00452.x; Carthew RW, 2009, CELL, V136, P642, DOI 10.1016/j.cell.2009.01.035; Celton JM, 2014, NEW PHYTOL, V203, P287, DOI 10.1111/nph.12787; Cerutti H, 2006, CURR GENET, V50, P81, DOI 10.1007/s00294-006-0078-x; Ciaudo C, 2009, PLOS GENET, V5, DOI 10.1371/journal.pgen.1000620; Cock JM, 2015, ADV MAR GENOMICS, V2, P335, DOI 10.1007/978-94-017-9642-2_16; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Cuperus JT, 2011, PLANT CELL, V23, P431, DOI 10.1105/tpc.110.082784; Czech B, 2008, NATURE, V453, P798, DOI 10.1038/nature07007; De Felippes FF, 2008, RNA, V14, P2455, DOI 10.1261/rna.1149408; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Erwin DH, 2011, SCIENCE, V334, P1091, DOI 10.1126/science.1206375; Field DJ, 2014, EVOL DEV, V16, P189, DOI 10.1111/ede.12081; Friedlander MR, 2012, NUCLEIC ACIDS RES, V40, P37, DOI 10.1093/nar/gkr688; Fromm B, 2013, MOL BIOL EVOL, V30, P2619, DOI 10.1093/molbev/mst155; Ghildiyal M, 2008, SCIENCE, V320, P1077, DOI 10.1126/science.1157396; Ghildiyal M, 2010, RNA, V16, P43, DOI 10.1261/rna.1972910; Ghildiyal M, 2009, NAT REV GENET, V10, P94, DOI 10.1038/nrg2504; Gobler CJ, 2011, P NATL ACAD SCI USA, V108, P4352, DOI 10.1073/pnas.1016106108; Goecks J, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-8-r86; Grimson A, 2008, NATURE, V455, P1193, DOI 10.1038/nature07415; Heimberg AM, 2008, P NATL ACAD SCI USA, V105, P2946, DOI 10.1073/pnas.0712259105; Heimberg AM, 2010, P NATL ACAD SCI USA, V107, P19379, DOI 10.1073/pnas.1010350107; Hertel J, 2006, BMC GENOMICS, V7, DOI 10.1186/1471-2164-7-25; Hinas A, 2007, NUCLEIC ACIDS RES, V35, P6714, DOI 10.1093/nar/gkm707; Kozomara A, 2011, NUCLEIC ACIDS RES, V39, pD152, DOI 10.1093/nar/gkq1027; Langenberger D, 2009, BIOINFORMATICS, V25, P2298, DOI 10.1093/bioinformatics/btp419; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Lau NC, 2001, SCIENCE, V294, P858, DOI 10.1126/science.1065062; Lee HC, 2010, MOL CELL, V38, P803, DOI 10.1016/j.molcel.2010.04.005; Li AL, 2007, CELL RES, V17, P212, DOI 10.1038/sj.cr.7310113; Li H, 2009, BIOINFORMATICS, V25, P1754, DOI 10.1093/bioinformatics/btp324; Liang Y, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-166; Lopez-Gomollon S, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-697; Lorenz R, 2011, ALGORITHM MOL BIOL, V6, DOI 10.1186/1748-7188-6-26; Lu J, 2008, NAT GENET, V40, P351, DOI 10.1038/ng.73; Luo GZ, 2012, BMC GENOMICS, V13, DOI 10.1186/1471-2164-13-727; Marco A, 2014, OPEN BIOL, V4, DOI 10.1098/rsob.140024; Marco A, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002402; Marco A, 2012, RNA BIOL, V9, P242, DOI 10.4161/rna.19160; Mattick JS, 2004, NAT REV GENET, V5, P316, DOI 10.1038/nrg1321; Molnar A, 2007, NATURE, V447, P1126, DOI 10.1038/nature05903; Mukherjee K, 2013, MOL BIOL EVOL, V30, P627, DOI 10.1093/molbev/mss263; Nozawa M, 2012, GENOME BIOL EVOL, V4, P230, DOI 10.1093/gbe/evs002; Nozawa M, 2010, GENOME BIOL EVOL, V2, P180, DOI 10.1093/gbe/evq009; Okamura K, 2008, NAT STRUCT MOL BIOL, V15, P354, DOI 10.1038/nsmb.1409; Ozsolak F, 2008, GENE DEV, V22, P3172, DOI 10.1101/gad.1706508; Pall GS, 2007, NUCLEIC ACIDS RES, V35, DOI 10.1093/nar/gkm112; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peterson KJ, 2009, BIOESSAYS, V31, P736, DOI 10.1002/bies.200900033; Piriyapongsa J, 2008, RNA, V14, P814, DOI 10.1261/rna.916708; Piriyapongsa J, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000203; Rajagopalan R, 2006, GENE DEV, V20, P3407, DOI 10.1101/gad.1476406; Reca I. B., 2012, PLOS GENET, V8; Rogato A, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-698; Sempere LF, 2006, J EXP ZOOL PART B, V306B, P575, DOI 10.1002/jez.b.21118; Shi WY, 2009, NAT STRUCT MOL BIOL, V16, P183, DOI 10.1038/nsmb.1536; Sperling EA, 2010, GEOBIOLOGY, V8, P24, DOI 10.1111/j.1472-4669.2009.00225.x; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tarver JE, 2013, MOL BIOL EVOL, V30, P2369, DOI 10.1093/molbev/mst133; Tarver JE, 2012, BIOESSAYS, V34, P857, DOI 10.1002/bies.201200055; Taylor RS, 2014, TRENDS PLANT SCI, V19, P175, DOI 10.1016/j.tplants.2013.11.008; Thomson RC, 2014, P NATL ACAD SCI USA, V111, pE3659, DOI 10.1073/pnas.1407207111; Voinnet O, 2009, CELL, V136, P669, DOI 10.1016/j.cell.2009.01.046; Wang B, 2013, J NUCLEIC ACIDS, V2013, DOI 10.1155/2013/951570; Wheeler B, 2013, CHROMOSOME RES, V21, P587, DOI 10.1007/s10577-013-9394-4; Wheeler BM, 2009, EVOL DEV, V11, P50, DOI 10.1111/j.1525-142X.2008.00302.x; Yang XZ, 2011, BIOINFORMATICS, V27, P2614, DOI 10.1093/bioinformatics/btr430; Zhang WX, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-8-r81; Zhao T, 2007, GENE DEV, V21, P1190, DOI 10.1101/gad.1543507	89	21	21	5	29	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0305-1048	1362-4962		NUCLEIC ACIDS RES	Nucleic Acids Res.	JUL 27	2015	43	13					6384	6398		10.1093/nar/gkv578			15	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	CP3JZ	WOS:000359776500022	26101255	DOAJ Gold, Green Published			2021-04-07	
J	Couceiro, L; Le Gac, M; Hunsperger, HM; Mauger, S; Destombe, C; Cock, JM; Ahmed, S; Coelho, SM; Valero, M; Peters, AF				Couceiro, Lucia; Le Gac, Mickael; Hunsperger, Heather M.; Mauger, Stephane; Destombe, Christophe; Cock, J. Mark; Ahmed, Sophia; Coelho, Susana M.; Valero, Myriam; Peters, Akira F.			Evolution and maintenance of haploid-diploid life cycles in natural populations: The case of the marine brown alga Ectocarpus	EVOLUTION			English	Article						Asexual reproduction; gametophyte; host specificity; niche differentiation; ploidy; seaweed; sporophyte	COMPARATIVE MORPHOLOGY; FUNCTIONAL-MORPHOLOGY; HISTORY; GENOME; PHAEOPHYCEAE; REPRODUCTION; SILICULOSUS; DIVERSITY; PERSISTENCE; TRANSITION	The evolutionary stability of haploid-diploid life cycles is still controversial. Mathematical models indicate that niche differences between ploidy phases may be a necessary condition for the evolution and maintenance of these life cycles. Nevertheless, experimental support for this prediction remains elusive. In the present work, we explored this hypothesis in natural populations of the brown alga Ectocarpus. Consistent with the life cycle described in culture, Ectocarpus crouaniorum in NW France and E. siliculosus in SW Italy exhibited an alternation between haploid gametophytes and diploid sporophytes. Our field data invalidated, however, the long-standing view of an isomorphic alternation of generations. Gametophytes and sporophytes displayed marked differences in size and, conforming to theoretical predictions, occupied different spatiotemporal niches. Gametophytes were found almost exclusively on the alga Scytosiphon lomentaria during spring whereas sporophytes were present year-round on abiotic substrata. Paradoxically, E. siliculosus in NW France exhibited similar habitat usage despite the absence of alternation of ploidy phases. Diploid sporophytes grew both epilithically and epiphytically, and this mainly asexual population gained the same ecological advantage postulated for haploid-diploid populations. Consequently, an ecological interpretation of the niche differences between haploid and diploid individuals does not seem to satisfactorily explain the evolution of the Ectocarpus life cycle.	[Couceiro, Lucia; Le Gac, Mickael; Hunsperger, Heather M.; Mauger, Stephane; Destombe, Christophe; Valero, Myriam] UPMC, Univ Paris 04, Evolutionary Biol & Ecol Algae, CNRS,UMI 3614,Stn Biol Roscoff,PUCCh,UACH, F-29688 Roscoff, France; [Cock, J. Mark; Ahmed, Sophia; Coelho, Susana M.] UPMC, Univ Paris 04, Algal Genet, CNRS,UMR 7139,Stn Biol Roscoff, F-29688 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France	Couceiro, L (corresponding author), UPMC, Univ Paris 04, Evolutionary Biol & Ecol Algae, CNRS,UMI 3614,Stn Biol Roscoff,PUCCh,UACH, 90074,Pl G Tessier, F-29688 Roscoff, France.	luciacouceirolopez@gmail.com	Valero, Myriam/M-6052-2019; Coelho, Susana/ABH-8166-2020; Valero, Myriam/C-7550-2011; Couceiro, Lucia/M-1851-2014	Valero, Myriam/0000-0002-9000-1423; Valero, Myriam/0000-0002-9000-1423; Couceiro, Lucia/0000-0003-4300-5744; Mauger, Stephane/0000-0002-8779-1516; Destombe, Christophe/0000-0001-5656-9659; Cock, J. Mark/0000-0002-2650-0383; Le Gac, Mickael/0000-0001-6451-5781; Couceiro, Lucia/0000-0001-9466-8164	Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission [ANR10-BLAN-1727, ANR12-JSV7-0008, ANR11-BSV7-00704]; University Pierre; Marie Curie (Emergence program), the Interreg program France (Channel)-England (project Marinexus); EUEuropean Commission [227799]; National Science FoundationNational Science Foundation (NSF) [OISE-0652093]	Funding for this work was provided by the Agence Nationale de la Recherche (projects Bi-Cycle, ANR10-BLAN-1727; Sexseaweed, ANR12-JSV7-0008; and Clonix, ANR11-BSV7-00704), the University Pierre and Marie Curie (Emergence program), the Interreg program France (Channel)-England (project Marinexus), the EU FP7 "capacities" specific program ASSEMBLE (grant no. 227799), and the National Science Foundation's International Research Experiences for Students program (grant no. OISE-0652093). The authors thank Biogenouest Core Facility for its technical assistance and the members of the Stazione Zoologica di Napoli for their advice and logistic support during sampling in Italy. The authors are most grateful to the editors and two anonymous reviewers for their constructive comments. The authors declare no conflicts of interest.	Agapow PM, 2001, MOL ECOL NOTES, V1, P101, DOI 10.1046/j.1471-8278.2000.00014.x; Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; [Anonymous], 1976, ARCH MICROBIOL, V109, P89; Arnaud-Haond S, 2007, MOL ECOL NOTES, V7, P15, DOI 10.1111/j.1471-8286.2006.01522.x; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; Bell G, 1997, BIOL J LINN SOC, V60, P21, DOI 10.1111/j.1095-8312.1997.tb01481.x; Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Bower F.O., 1908, ORIGIN LAND FLORA TH; Carney Laura T., 2006, Algae, V21, P161; Cock JM, 2014, CURR OPIN PLANT BIOL, V17, P1, DOI 10.1016/j.pbi.2013.09.004; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Coluccio AE, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002873; CROW JAMES F., 1965, AMER NATUR, V99, P439, DOI 10.1086/282389; CUNNINGHAM EM, 1993, J EXP MAR BIOL ECOL, V171, P1, DOI 10.1016/0022-0981(93)90136-C; DESTOMBE C, 1989, J EVOLUTION BIOL, V2, P317, DOI 10.1046/j.1420-9101.1989.2050317.x; Dorken ME, 2001, J ECOL, V89, P339, DOI 10.1046/j.1365-2745.2001.00558.x; Edwards MS, 2000, ECOLOGY, V81, P2404, DOI 10.2307/177463; Feldmann J, 1972, B SOC BOT FRANCE, V119, P7; Fierst J, 2005, J PHYCOL, V41, P238, DOI 10.1111/j.1529-8817.2005.04024.x; Frada M, 2008, P NATL ACAD SCI USA, V105, P15944, DOI 10.1073/pnas.0807707105; Fritsch FE, 1942, ANN BOT-LONDON, V6, P397, DOI 10.1093/oxfordjournals.aob.a088413; Fritsch FE, 1942, ANN BOT-LONDON, V6, P533, DOI 10.1093/oxfordjournals.aob.a088420; Halkett F, 2005, TRENDS ECOL EVOL, V20, P194, DOI 10.1016/j.tree.2005.01.001; HAY ME, 1981, ECOLOGY, V62, P739, DOI 10.2307/1937742; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hughes JS, 1999, AM NAT, V154, P306, DOI 10.1086/303241; Immler S, 2014, AM NAT, V183, P96, DOI 10.1086/674025; JENKINS CD, 1995, EVOLUTION, V49, P512, DOI [10.2307/2410275, 10.1111/j.1558-5646.1995.tb02283.x]; KLINGER T, 1993, TRENDS ECOL EVOL, V8, P256, DOI 10.1016/0169-5347(93)90202-Z; KNIGHT MARGERY, 1929, TRANS ROY SOC EDINBURGH, V56, P307; LITTLER MM, 1983, J PHYCOL, V19, P425, DOI 10.1111/j.0022-3646.1983.00425.x; Lotka A. J., 1925, ELEMENTS PHYS BIOL; LUBCHENCO J, 1980, ECOLOGY, V61, P676, DOI 10.2307/1937433; Mable BK, 1998, BIOESSAYS, V20, P453, DOI 10.1002/(SICI)1521-1878(199806)20:6<453::AID-BIES3>3.0.CO;2-N; Mauger S, 2012, PRIME RES BIOTECHNOL, V2, P40; MORRIS O P, 1974, British Phycological Journal, V9, P269; Muller D. G., 1963, PUBBLICAZIONI STAZIO, V33, P310; Muller D. G, 1967, PLANTA, V75, P39; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1977, BRIT PHYCOL J, V12, P131; ORR HA, 1994, GENETICS, V136, P1475; Otto SP, 2008, CURR BIOL, V18, pR1121, DOI 10.1016/j.cub.2008.09.039; Otto SP, 1996, BIOL J LINN SOC, V57, P197, DOI 10.1111/j.1095-8312.1996.tb00309.x; OTTO SP, 1992, GENETICS, V131, P745; Pacheco-Ruiz I, 1999, HYDROBIOLOGIA, V399, P159; PAPENFUSS GEORGE F., 1933, SCIENCE, V77, P390, DOI 10.1126/science.77.1999.390; Papenfuss GF, 1934, BOT GAZ, V96, P421; PAQUIN C, 1983, NATURE, V302, P495, DOI 10.1038/302495a0; Peakall R, 2006, MOL ECOL NOTES, V6, P288, DOI 10.1111/j.1471-8286.2005.01155.x; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters AF, 2015, CRYPTOGAMIE ALGOL, V36, P3, DOI 10.7872/crya.v36.iss1.2015.3; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Polifrone M, 2012, AN JARDIN BOT MADRID, V69, P247, DOI 10.3989/ajbm.2325; RAYMOND M, 1995, J HERED, V86, P248, DOI 10.1093/oxfordjournals.jhered.a111573; RICE WR, 1989, EVOLUTION, V43, P223, DOI 10.1111/j.1558-5646.1989.tb04220.x; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; SLOCUM CJ, 1980, J EXP MAR BIOL ECOL, V46, P99, DOI 10.1016/0022-0981(80)90095-7; STEBBINS GL, 1980, AM NAT, V115, P342, DOI 10.1086/283565; TAYLOR PR, 1984, MAR ECOL PROG SER, V18, P295, DOI 10.3354/meps018295; Thornber CS, 2006, INTEGR COMP BIOL, V46, P605, DOI 10.1093/icb/icl018; VALERO M, 1992, TRENDS ECOL EVOL, V7, P25, DOI 10.1016/0169-5347(92)90195-H; Van Oosterhout C, 2004, MOL ECOL NOTES, V4, P535, DOI 10.1111/j.1471-8286.2004.00684.x; Verges A, 2008, ECOLOGY, V89, P1334, DOI 10.1890/07-0248.1; Volterra V, 1926, NATURE, V118, P558, DOI 10.1038/118558a0; WILLSON MF, 1981, ANN MO BOT GARD, V68, P275, DOI 10.2307/2398799; ZUPAN JR, 1990, J PHYCOL, V26, P232, DOI 10.1111/j.0022-3646.1990.00232.x	73	29	29	3	72	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0014-3820	1558-5646		EVOLUTION	Evolution	JUL	2015	69	7					1808	1822		10.1111/evo.12702			15	Ecology; Evolutionary Biology; Genetics & Heredity	Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity	CN5WP	WOS:000358503800013	26096000				2021-04-07	
J	Wideman, JG; Moore, BP				Wideman, Jeremy G.; Moore, Blake P.			The Evolutionary History of MAPL (Mitochondria-Associated Protein Ligase) and Other Eukaryotic BAM/GIDE Domain Proteins	PLOS ONE			English	Article							E3 UBIQUITIN LIGASE; SALMON SALMO-SALAR; MAXIMUM-LIKELIHOOD; MIXED MODELS; MULAN; MITOPHAGY; REPLACEMENT; PEROXISOMES; BIOGENESIS; ACTIVATOR	MAPL (mitochondria-associated protein ligase, also called MULAN/GIDE/MUL1) is a multifunctional mitochondrial outer membrane protein found in human cells that contains a unique BAM (beside a membrane) domain and a C-terminal RING-finger domain. MAPL has been implicated in several processes that occur in animal cells such as NF-kB activation, innate immunity and antiviral signaling, suppression of PINK1/parkin defects, mitophagy in skeletal muscle, and caspase-dependent apoptosis. Previous studies demonstrated that the BAM domain is present in diverse organisms in which most of these processes do not occur, including plants, archaea, and bacteria. Thus the conserved function of MAPL and its BAM domain remains an open question. In order to gain insight into its conserved function, we investigated the evolutionary origins of MAPL by searching for homologues in predicted proteomes of diverse eukaryotes. We show that MAPL proteins with a conserved BAM-RING architecture are present in most animals, protists closely related to animals, a single species of fungus, and several multicellular plants and related green algae. Phylogenetic analysis demonstrated that eukaryotic MAPL proteins originate from a common ancestor and not from independent horizontal gene transfers from bacteria. We also determined that two independent duplications of MAPL occurred, one at the base of multicellular plants and another at the base of vertebrates. Although no other eukaryote genome examined contained a verifiable MAPL orthologue, BAM domain-containing proteins were identified in the protists Bigelowiella natans and Ectocarpus siliculosis. Phylogenetic analyses demonstrated that these proteins are more closely related to prokaryotic BAM proteins and therefore likely arose from independent horizontal gene transfers from bacteria. We conclude that MAPL proteins with BAM-RING architectures have been present in the holozoan and viridiplantae lineages since their very beginnings. Our work paves the way for future studies into MAPL function in alternative model organisms like Capsaspora owczarzaki and Chlamydomonas reinhardtii that will help to answer the question of MAPL's ancestral function in ways that cannot be answered by studying animal cells alone.	[Wideman, Jeremy G.; Moore, Blake P.] Univ Alberta, Augustana Fac, Dept Sci, Camrose, AB T4V 2R3, Canada; [Wideman, Jeremy G.] Univ Alberta, Fac Med & Dent, Dept Cell Biol, Edmonton, AB T6G 2H7, Canada	Wideman, JG (corresponding author), Univ Alberta, Augustana Fac, Dept Sci, Camrose, AB T4V 2R3, Canada.	jeremy.grant.wideman@gmail.com		Wideman, Jeremy/0000-0002-4426-9533			Abascal F, 2005, BIOINFORMATICS, V21, P2104, DOI 10.1093/bioinformatics/bti263; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Ambivero CT, 2014, CELL SIGNAL, V26, P2921, DOI 10.1016/j.cellsig.2014.09.004; Andrade-Navarro MA, 2009, CURR OPIN CELL BIOL, V21, P560, DOI 10.1016/j.ceb.2009.04.005; Bae SH, 2012, CELL RES, V22, P873, DOI 10.1038/cr.2012.38; Braschi E, 2010, CURR BIOL, V20, P1310, DOI 10.1016/j.cub.2010.05.066; Braschi E, 2009, EMBO REP, V10, P748, DOI 10.1038/embor.2009.86; Cilenti L, 2014, BBA-MOL CELL RES, V1843, P1295, DOI 10.1016/j.bbamcr.2014.03.027; Edgar RC, 2004, BMC BIOINFORMATICS, V5, P1, DOI 10.1186/1471-2105-5-113; Gray MW, 2014, COLD SPRING HARB PER, V6; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Huynen MA, 2013, BBA-BIOENERGETICS, V1827, P224, DOI 10.1016/j.bbabio.2012.08.001; Jenkins K, 2013, IMMUNOL CELL BIOL, V91, P321, DOI 10.1038/icb.2013.7; Kulp A, 2010, ANNU REV MICROBIOL, V64, P163, DOI 10.1146/annurev.micro.091208.073413; Le SQ, 2008, MOL BIOL EVOL, V25, P1307, DOI 10.1093/molbev/msn067; Li W, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0001487; Lokireddy S, 2012, CELL METAB, V16, P613, DOI 10.1016/j.cmet.2012.10.005; McLelland GL, 2014, EMBO J, V33, P282, DOI 10.1002/embj.201385902; Mohanty A, 2013, FRONT PHYSIOL, V4, DOI 10.3389/fphys.2013.00268; Neuspiel M, 2008, CURR BIOL, V18, P102, DOI 10.1016/j.cub.2007.12.038; Ronquist F, 2003, BIOINFORMATICS, V19, P1572, DOI 10.1093/bioinformatics/btg180; Soubannier V, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0052830; Stamatakis A, 2006, BIOINFORMATICS, V22, P2688, DOI 10.1093/bioinformatics/btl446; Sugiura A., 2014, EMBO J; Tacchi L, 2012, DEV COMP IMMUNOL, V38, P545, DOI 10.1016/j.dci.2012.08.004; Tacchi L, 2011, MOL IMMUNOL, V49, P558, DOI 10.1016/j.molimm.2011.10.005; Yun JN, 2014, ELIFE, V3, DOI 10.7554/eLife.01958; Zemirli N, 2014, FEBS J, V281, P3095, DOI 10.1111/febs.12846; Zhang BC, 2008, CELL RES, V18, P900, DOI 10.1038/cr.2008.75	29	2	2	0	8	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	JUN 5	2015	10	6							e0128795	10.1371/journal.pone.0128795			12	Multidisciplinary Sciences	Science & Technology - Other Topics	CJ7CR	WOS:000355652200101	26047467	DOAJ Gold, Green Published			2021-04-07	
J	Lipinska, A; Cormier, A; Luthringer, R; Peters, AF; Corre, E; Gachon, CM; Cock, JM; Coelho, SM				Lipinska, Agnieszka; Cormier, Alexandre; Luthringer, Remy; Peters, Akira F.; Corre, Erwan; Gachon, Claire M. M.; Cock, J. Mark; Coelho, Susana M.			Sexual Dimorphism and the Evolution of Sex-Biased Gene Expression in the Brown Alga Ectocarpus	MOLECULAR BIOLOGY AND EVOLUTION			English	Article						sex-biased gene expression; haploid-diploid life cycle; brown algae; UV sex chromosomes	DOSAGE COMPENSATION; X-CHROMOSOME; CODON USAGE; PHYLOGENETIC ANALYSIS; MOLECULAR EVOLUTION; GENOME; SELECTION; FASTER; DYNAMICS; RATES	Males and females often have marked phenotypic differences, and the expression of these dissimilarities invariably involves sex differences in gene expression. Sex-biased gene expression has been well characterized in animal species, where a high proportion of the genome may be differentially regulated in males and females during development. Male-biased genes tend to evolve more rapidly than female-biased genes, implying differences in the strength of the selective forces acting on the two sexes. Analyses of sex-biased gene expression have focused on organisms that exhibit separate sexes during the diploid phase of the life cycle (diploid sexual systems), but the genetic nature of the sexual system is expected to influence the evolutionary trajectories of sex-biased genes. We analyze here the patterns of sex-biased gene expression in Ectocarpus, a brown alga with haploid sex determination (dioicy) and a low level of phenotypic sexual dimorphism. In Ectocarpus, female-biased genes were found to be evolving as rapidly as male-biased genes. Moreover, genes expressed at fertility showed faster rates of evolution than genes expressed in immature gametophytes. Both male- and female-biased genes had a greater proportion of sites experiencing positive selection, suggesting that their accelerated evolution is at least partly driven by adaptive evolution. Gene duplication appears to have played a significant role in the generation of sex-biased genes in Ectocarpus, expanding previous models that propose this mechanism for the resolution of sexual antagonism in diploid systems. The patterns of sex-biased gene expression in Ectocarpus are consistent both with predicted characteristics of UV (haploid) sexual systems and with the distinctive aspects of this organism's reproductive biology.	[Lipinska, Agnieszka; Cormier, Alexandre; Luthringer, Remy; Cock, J. Mark; Coelho, Susana M.] Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, UMR 8227,Algal Genet,Integrat Biol Marine Models, F-29688 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, Santec, France; [Corre, Erwan] UPMC, CNRS, FR2424, Stn Biol Roscoff,Abims Platform, Roscoff, France; [Gachon, Claire M. M.] Scottish Assoc Marine Sci, Scottish Marine Inst, Microbial & Mol Biol Dept, Oban, Argyll, Scotland	Coelho, SM (corresponding author), Univ Paris 06, Sorbonne Univ, CNRS,Stn Biol Roscoff, UMR 8227,Algal Genet,Integrat Biol Marine Models, F-29688 Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020; corre, erwan/O-4669-2019	corre, erwan/0000-0001-6354-2278; Cock, J. Mark/0000-0002-2650-0383; Gachon, Claire/0000-0002-3702-7472; Cormier, Alexandre/0000-0002-7775-8413	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission; University Pierre and Marie Curie Emergence program; Interreg program France (Channel)-England (project Marinexus); NERC National Biomolecular Analysis Facility (NBAF-E); NERCUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/J00460X/1, NE/L013223/1]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NBAF010003, NE/L013223/1, NE/J00460X/1, dml010007] Funding Source: researchfish	This work was supported by the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (Project SEXSEAWEED and project IDEALG), the University Pierre and Marie Curie Emergence program, the Interreg program France (Channel)-England (project Marinexus), the NERC National Biomolecular Analysis Facility (NBAF-E), and the NERC grant NE/J00460X/1 and NE/L013223/1. The authors thank Lieven Sterck for help with the analysis of duplicated genes, Julie Jaquiery for helpful discussions, D. Marie and D. Voulot for help with the citometry mesurements, and K. Kogame for the sampling and culture of Scytosiphon lomentaria.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Albritton SE, 2014, GENETICS, V197, P865, DOI 10.1534/genetics.114.163311; Anders S, 2015, BIOINFORMATICS, V31, P166, DOI 10.1093/bioinformatics/btu638; Anders S, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-10-r106; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Arunkumar KP, 2009, GENETICS, V182, P493, DOI 10.1534/genetics.108.099994; Arunkumar R, 2013, MOL BIOL EVOL, V30, P2475, DOI 10.1093/molbev/mst149; Assis R, 2012, GENOME BIOL EVOL, V4, P1189, DOI 10.1093/gbe/evs093; Avila V, 2014, GENOME BIOL EVOL, V6, P2968, DOI 10.1093/gbe/evu229; Bachtrog D, 2011, TRENDS GENET, V27, P350, DOI 10.1016/j.tig.2011.05.005; Bachtrog D, 2010, CURR BIOL, V20, P1476, DOI 10.1016/j.cub.2010.06.076; Barker MS, 2005, PLOS GENET, V1, P527, DOI 10.1371/journal.pgen.0010057; Begun DJ, 2007, PLOS BIOL, V5, P2534, DOI 10.1371/journal.pbio.0050310; Bellott DW, 2010, NATURE, V466, P612, DOI 10.1038/nature09172; Bohne A, 2014, GENOME BIOL EVOL, V6, P2567, DOI 10.1093/gbe/evu200; BULL JJ, 1978, AM NAT, V112, P245, DOI 10.1086/283267; Campos JL, 2014, MOL BIOL EVOL, V31, P1010, DOI 10.1093/molbev/msu056; CHARLESWORTH B, 1987, AM NAT, V130, P113, DOI 10.1086/284701; Charlesworth B, 2014, EVOLUTION, V68, P1339, DOI 10.1111/evo.12364; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Conesa Ana, 2008, Int J Plant Genomics, V2008, P619832, DOI 10.1155/2008/619832; Connallon T, 2011, GENETICS, V187, P919, DOI 10.1534/genetics.110.123729; Duret L, 2000, TRENDS GENET, V16, P287, DOI 10.1016/S0168-9525(00)02041-2; Duret L, 2000, MOL BIOL EVOL, V17, P68, DOI 10.1093/oxfordjournals.molbev.a026239; Ellegren H, 2007, NAT REV GENET, V8, P689, DOI 10.1038/nrg2167; Gallach M, 2011, TRENDS ECOL EVOL, V26, P222, DOI 10.1016/j.tree.2011.02.004; Gerstein AC, 2011, J EVOLUTION BIOL, V24, P531, DOI 10.1111/j.1420-9101.2010.02188.x; Gossmann TI, 2014, MOL BIOL EVOL, V31, P574, DOI 10.1093/molbev/mst226; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Grath S, 2012, GENOME BIOL EVOL, V4, P346, DOI 10.1093/gbe/evs012; Haerty W, 2007, GENETICS, V177, P1321, DOI 10.1534/genetics.107.078865; Hambuch TM, 2005, GENETICS, V170, P1691, DOI 10.1534/genetics.104.038109; Hastings KEM, 1996, J MOL EVOL, V42, P631, DOI 10.1007/BF02338796; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Innocenti P, 2010, PLOS BIOL, V8, DOI 10.1371/journal.pbio.1000335; Jaquiery J, 2013, PLOS GENET, V9, DOI 10.1371/journal.pgen.1003690; Jiang ZF, 2009, GENETICS, V183, P1175, DOI 10.1534/genetics.109.105775; Jordan CY, 2012, EVOLUTION, V66, P505, DOI 10.1111/j.1558-5646.2011.01448.x; Kayserili MA, 2012, PLOS GENET, V8, DOI 10.1371/journal.pgen.1003200; Khil PP, 2004, NAT GENET, V36, P642, DOI 10.1038/ng1368; Kim D, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-4-r36; Kirkpatrick M, 2014, GENETICS, V197, P531, DOI 10.1534/genetics.113.156026; KONDRASHOV AS, 1991, NATURE, V351, P314, DOI 10.1038/351314a0; Kousathanas A, 2014, GENETICS, V196, P1131, DOI 10.1534/genetics.113.158246; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; Leder EH, 2010, MOL BIOL EVOL, V27, P1495, DOI 10.1093/molbev/msq031; Li L, 2003, GENOME RES, V13, P2178, DOI 10.1101/gr.1224503; Lipinska AP, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-909; Luthringer R, 2015, PERSPECT PHYCOL, V1, P11; Mank JE, 2007, MOL BIOL EVOL, V24, P2698, DOI 10.1093/molbev/msm208; Mank JE, 2013, TRENDS GENET, V29, P677, DOI 10.1016/j.tig.2013.07.005; Mank JE, 2010, MOL BIOL EVOL, V27, P1570, DOI 10.1093/molbev/msq042; Mank JE, 2009, BIOL LETTERS, V5, P409, DOI 10.1098/rsbl.2008.0732; Martins MJF, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-294; Meisel RP, 2012, PLOS GENET, V8, DOI 10.1371/journal.pgen.1003013; Meisel RP, 2011, MOL BIOL EVOL, V28, P1893, DOI 10.1093/molbev/msr010; Muller DG, 1975, LYNGB ARCH PROTISTEN, V117, P297; Nagasato C, 2002, J CELL SCI, V115, P2541; ORR HA, 1994, GENETICS, V136, P1475; Otto SP, 2011, TRENDS GENET, V27, P358, DOI 10.1016/j.tig.2011.05.001; Parsch J, 2013, NAT REV GENET, V14, P83, DOI 10.1038/nrg3376; Perry JC, 2014, MOL BIOL EVOL, V31, P1206, DOI 10.1093/molbev/msu072; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Pointer MA, 2013, PLOS GENET, V9, DOI 10.1371/journal.pgen.1003697; Presgraves DC, 2008, TRENDS GENET, V24, P336, DOI 10.1016/j.tig.2008.04.007; Puigbo P, 2008, BIOL DIRECT, V3, DOI 10.1186/1745-6150-3-38; RICE WR, 1984, EVOLUTION, V38, P735, DOI [10.2307/2408385, 10.1111/j.1558-5646.1984.tb00346.x]; SCHMID CE, 1994, PLANT SCI, V102, P61, DOI 10.1016/0168-9452(94)90021-3; Sharma E, 2014, BMC GENOMICS, V15, DOI 10.1186/1471-2164-15-400; Smith G, 2014, GENOME BIOL EVOL, V6, P526, DOI 10.1093/gbe/evu035; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Suyama M, 2006, NUCLEIC ACIDS RES, V34, pW609, DOI 10.1093/nar/gkl315; Szovenyi P, 2013, MOL BIOL EVOL, V30, P1929, DOI 10.1093/molbev/mst095; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Uebbing S, 2013, GENOME BIOL EVOL, V5, P1555, DOI 10.1093/gbe/evt114; Vicoso B, 2013, P NATL ACAD SCI USA, V110, P6453, DOI 10.1073/pnas.1217027110; Vicoso B, 2009, EVOLUTION, V63, P2413, DOI 10.1111/j.1558-5646.2009.00719.x; Whittle CA, 2013, MOL BIOL EVOL, V30, P2435, DOI 10.1093/molbev/mst143; Wyman MJ, 2012, EVOLUTION, V66, P1556, DOI 10.1111/j.1558-5646.2011.01525.x; Yanai I, 2005, BIOINFORMATICS, V21, P650, DOI 10.1093/bioinformatics/bti042; Yang ZH, 2000, J MOL EVOL, V51, P423, DOI 10.1007/s002390010105; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847; Zhang Z, 2004, MOL BIOL EVOL, V21, P2130, DOI 10.1093/molbev/msh223	88	52	53	1	46	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0737-4038	1537-1719		MOL BIOL EVOL	Mol. Biol. Evol.	JUN	2015	32	6					1581	1597		10.1093/molbev/msv049			17	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	CK5JJ	WOS:000356259600018	25725430	Bronze, Green Published			2021-04-07	
J	Tsirigoti, A; Beakes, GW; Herve, C; Gachon, CMM; Katsaros, C				Tsirigoti, Amerssa; Beakes, Gordon W.; Herve, Cecile; Gachon, Claire M. M.; Katsaros, Christos			Attachment, penetration and early host defense mechanisms during the infection of filamentous brown algae by Eurychasma dicksonii	PROTOPLASMA			English	Article						Brown algae; Eurychasma; Host defense; Injection mechanism; Oomycete; Parasite	ECTOCARPUS-SILICULOSUS; OOMYCETE; MARINE; DIFFERENTIATION; ULTRASTRUCTURE; SUSCEPTIBILITY; CROUANIORUM; GERMINATION; PARASITE; CYTOLOGY	Eurychasma dicksonii is one of the most common and widespread marine pathogens and attacks a broad spectrum of more than 45 brown algal species. The present study focuses on the mechanism used by the pathogen to attach on the host cell wall and force its way into algal cells. Ultrastructural examination revealed a needle-like structure which develops within the attached spore and extends along its main axis. Particular cell wall modifications are present at the basal part of the spore (adhesorium pad) and guide the needle-like tool to penetrate perpendicularly the host cell wall. The unique injection mechanism is shared with Haptoglossa species which suggests that this is an important characteristic of early diverging oomycetes. Furthermore, the encystment and adhesion mechanism of E. dicksonii shows significant similarities with other oomycetes, some of which are plant pathogens. Staining and immunolabelling techniques showed the deposition of beta-1,3-glucans on the host cell wall at the pathogen penetration site, a strategy similar to physical responses previously described only in infected plant cells. It is assumed that the host defense in terms of callose-like deposition is an ancient response to infection.	[Tsirigoti, Amerssa; Katsaros, Christos] Univ Athens, Fac Biol, Dept Bot, Athens 15784, Greece; [Beakes, Gordon W.] Newcastle Univ, Dept Biol & Nutr Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England; [Herve, Cecile] Univ Paris 06, Sorbonne Univ, Integrat Biol Marine Models, CNRS,UMR 8227,Stn Biol, F-29688 Roscoff, France; [Gachon, Claire M. M.] Scottish Marine Inst, Culture Collect Algae & Protozoa, Scottish Assoc Marine Sci, Oban PA 37 1QA, Argyll, Scotland	Katsaros, C (corresponding author), Univ Athens, Fac Biol, Dept Bot, Athens 15784, Greece.	christos.katsaros@biol.uoa.gr		Herve, Cecile/0000-0001-6649-8137; Gachon, Claire/0000-0002-3702-7472	British Phycological Society; Federation of European Microbiological Societies (F.E.M.S.); European CommissionEuropean CommissionEuropean Commission Joint Research Centre [227799]; TOTAL Foundation; UK NERCUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/J00460X/1]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/J00460X/1, dml010007] Funding Source: researchfish	We gratefully acknowledge funding from the British Phycological Society and the Federation of European Microbiological Societies (F.E.M.S.) for the short-term grants that enabled AT to work in Roscoff and Newcastle. AT's visit to Station Biologique de Roscoff (France) and the supply of the biological material by CCAP (Culture Collection of Algae and Protozoa, Oban, Scotland) were supported by the European Commission through under the FP7 'capacities' specific program ASSEMBLE (grant no. 227799). We also acknowledge Prof. Kostas Fasseas (Agricultural University of Athens) for his precious help with SEM and Emily Olesin for technical support. This work was partially supported by TOTAL Foundation (project title "Brown algal biodiversity and ecology in the eastern Mediterranean sea"). CMMG is funded by a UK NERC grant No NE/J00460X/1.	AIST JR, 1971, CAN J BOTANY, V49, P2023, DOI 10.1139/b71-284; Andersen R, 2005, ALGAL CULTURING TECH; Apostolakos P, 2010, NEW PHYTOL, V186, P623, DOI 10.1111/j.1469-8137.2010.03206.x; Bacic A, 2009, CHEMISTRY, BIOCHEMISTRY, AND BIOLOGY OF (1-->3)-BETA-GLUCANS AND RELATED POLYSACCHARIDES, P1; BEAKES G, 1983, CAN J BOT, V61, P603, DOI 10.1139/b83-068; Beakes GW, 2014, FUNGAL BIOL-UK, V118, P527, DOI 10.1016/j.funbio.2014.04.005; BEAKES GW, 1989, SYST ASSOC SPEC VOL, V38, P325; Beakes GW, 1998, FUNGAL GENET BIOL, V24, P45, DOI 10.1006/fgbi.1998.1072; Beakes GW, 2000, MYCOL RES, V104, P1258, DOI 10.1017/S0953756200003178; BURR AW, 1994, PROTOPLASMA, V181, P142, DOI 10.1007/BF01666393; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Grenville-Briggs L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0024500; Hakariya M, 2002, MYCOSCIENCE, V438, P119; Heath IB, 1995, GROWING FUNGUS, P99; HELD AA, 1973, CAN J BOT, V51, P1825, DOI 10.1139/b73-234; Katsaros C, 1997, PHYCOLOGIA, V36, P60, DOI 10.2216/i0031-8884-36-1-60.1; Klochkova TA, 2012, J APPL PHYCOL, V24, P135, DOI 10.1007/s10811-011-9661-8; LEHNEN LP, 1989, PROTOPLASMA, V149, P163, DOI 10.1007/BF01322988; Luna E, 2011, MOL PLANT MICROBE IN, V24, P183, DOI 10.1094/MPMI-07-10-0149; Melida H, 2013, EUKARYOT CELL, V12, P194, DOI 10.1128/EC.00288-12; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Muller DG, 2008, CAH BIOL MAR, V49, P59; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; OVERTON SV, 1983, CAN J BOT, V61, P1165, DOI 10.1139/b83-123; Parker BC, 1964, PHYCOLOGIA, V4, P27; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; ROBB EJ, 1982, SCIENCE, V218, P1221, DOI 10.1126/science.218.4578.1221; Robold AV, 2005, CURR GENET, V47, P307, DOI 10.1007/s00294-004-0559-8; SCHNEPF E, 1978, ARCH MICROBIOL, V116, P141, DOI 10.1007/BF00406029; SCHNEPF E, 1978, CAN J BOT, V56, P1315, DOI 10.1139/b78-149; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; TEMMINK JHM, 1969, CAN J BOTANY, V47, P421, DOI 10.1139/b69-057; Tsirigoti A, 2014, PLANT BIOLOGY, V16, P272, DOI 10.1111/plb.12041; Underwood W, 2012, FRONT PLANT SCI, V3, DOI 10.3389/fpls.2012.00085; Uppalapati SR, 2001, BOT MAR, V44, P139, DOI 10.1515/BOT.2001.019; Voigt CA, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00168; WILLOUGHBY LG, 1977, T BRIT MYCOL SOC, V69, P133, DOI 10.1016/S0007-1536(77)80124-1	39	10	11	1	20	SPRINGER WIEN	WIEN	SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA	0033-183X	1615-6102		PROTOPLASMA	Protoplasma	MAY	2015	252	3					845	856		10.1007/s00709-014-0721-1			12	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	CG3ZV	WOS:000353220200010	25385261				2021-04-07	
J	Liu, F; Pang, SJ				Liu, Feng; Pang, Shaojun			Mitochondrial phylogenomics reveals a close relationship between Petalonia fascia (Scytosiphonaceae, Phaeophyceae) and Ectocarpus siliculosus	JOURNAL OF APPLIED PHYCOLOGY			English	Article						Petalonia fascia; Mitochondrial genome; Brown algae; Phaeophyceae; Scytosiphonaceae	DNA-SEQUENCE DATA; MAXIMUM-LIKELIHOOD; GENOMES; ALGAE; EVOLUTION	The complete mitochondrial (mt) genome sequence of the brown alga, Petalonia fascia (O.F. Muller) Kuntze was determined and analyzed in this study. The 38,053-bp mt genome contains 68 genes, including 3 ribosomal RNA genes, 25 transfer RNA genes, 35 protein-coding genes, and 5 unidentified open reading frames (ORFs). The noncoding DNA represents 6.50 % of the total genome. The average size of intergenic spacers is 44.2 nucleotides with a range of 0 to 389 nucleotides. The nad11 gene in P. fascia, unlike other coding genes with a methionine (ATG) start codon, commences with a typical TTG codon. The P. fascia mt genome exhibits novel genome organization and gene arrangement, which are similar to those of Ectocarpus siliculosus. Phylogenetic analyses based on 35 protein-coding genes reveal that P. fascia has a close evolutionary relationship with E. siliculosus compared with other brown algae analyzed. The molecular data presented provide a useful tool for classification, evolutionary as well as population genetic studies in Scytosiphonaceae.	[Liu, Feng; Pang, Shaojun] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China	Liu, F (corresponding author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.	liufeng@qdio.ac.cn; sjpang@qdio.ac.cn			National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41206146]; 863 Hi-Tech Research and Development Program of ChinaNational High Technology Research and Development Program of China [2012AA10A413]; Scientific Research Foundation for Outstanding Young Scientists of Shandong Province [BS2013HZ004]; Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, State Oceanic Administration [MATHAB201408]; Open Research Fund of Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, P. R. China [K201311]	The authors thank Claire M.M. Gachon and two anonymous reviewers for their helpful advice, and Jiaqi Wang, Xige Jiang, and Changbin Sun for their assistance in algal collection. This investigation was supported by the National Natural Science Foundation of China (No. 41206146), the 863 Hi-Tech Research and Development Program of China (No. 2012AA10A413), the Scientific Research Foundation for Outstanding Young Scientists of Shandong Province (No. BS2013HZ004), the Open Research Fund of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, State Oceanic Administration (No. MATHAB201408), and the Open Research Fund of Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, P. R. China (No. K201311).	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Bittner L, 2008, MOL PHYLOGENET EVOL, V49, P211, DOI 10.1016/j.ympev.2008.06.018; Boore JL, 1999, NUCLEIC ACIDS RES, V27, P1767, DOI 10.1093/nar/27.8.1767; Burger G, 2003, TRENDS GENET, V19, P709, DOI 10.1016/j.tig.2003.10.012; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; CHENG S, 1994, NATURE, V369, P684, DOI 10.1038/369684a0; Cho Ga Youn, 2006, Algae, V21, P175; Cho Ga Youn, 2001, Algae, V16, P145; CUMMINGS MP, 1995, MOL BIOL EVOL, V12, P814; Draisma SGA, 2001, J PHYCOL, V37, P586, DOI 10.1046/j.1529-8817.2001.037004586.x; Draisma SGA, 2010, J PHYCOL, V46, P1329, DOI 10.1111/j.1529-8817.2010.00891.x; GaYounCho, 2002, ALGAE, V17, P135; Gray MW, 2001, GENOME BIOL, V2; Guiry M.D., 2014, ALGAEBASE; Hall TA.., 1999, NUCL ACIDS S SERIES, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; KIMURA M, 1980, J MOL EVOL, V16, P111, DOI 10.1007/BF01731581; Kogame K, 1999, PHYCOLOGIA, V38, P496, DOI 10.2216/i0031-8884-38-6-496.1; Kogame K, 2005, EUR J PHYCOL, V40, P313, DOI 10.1080/09670260500193008; Kogame K, 2011, PHYCOLOGIA, V50, P563, DOI 10.2216/10-75.1; Lang BF, 1999, ANNU REV GENET, V33, P351, DOI 10.1146/annurev.genet.33.1.351; Li TY, 2015, MITOCHONDR DNA, V26, P953, DOI 10.3109/19401736.2013.865172; Liu F, 2016, MITOCHONDRIAL DNA A, V27, P1158, DOI 10.3109/19401736.2014.936417; Liu F, 2016, MITOCHONDRIAL DNA A, V27, P1129, DOI 10.3109/19401736.2014.933333; Liu F, 2015, J APPL PHYCOL, V27, P469, DOI 10.1007/s10811-014-0295-5; Lohse M, 2013, NUCLEIC ACIDS RES, V41, pW575, DOI 10.1093/nar/gkt289; Oudot-le Secq MP, 2002, EUR J PHYCOL, V37, P163, DOI 10.1017/S0967026202003542; Oudot-Le Secq MP, 2001, J MOL EVOL, V53, P80, DOI 10.1007/s002390010196; Qu JQ, 2015, MITOCHONDR DNA, V26, P919, DOI 10.3109/19401736.2013.863290; Rousseau F, 2001, CR ACAD SCI III-VIE, V324, P305, DOI 10.1016/S0764-4469(01)01306-3; Rousseau F, 1999, CRYPTOGAMIE ALGOL, V20, P5, DOI 10.1016/S0181-1568(99)80002-6; Schattner P, 2005, NUCLEIC ACIDS RES, V33, pW686, DOI 10.1093/nar/gki366; Secq MPO, 2006, CURR GENET, V49, P47, DOI 10.1007/s00294-005-0031-4; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Thompson JD, 1997, NUCLEIC ACIDS RES, V25, P4876, DOI 10.1093/nar/25.24.4876; Toste MF, 2003, J PHYCOL, V39, P1268; Tseng CK, 2009, SEAWEEDS YELLOW SEA, P367; Turmel M, 2003, PLANT CELL, V15, P1888, DOI 10.1105/tpc.013169; Whelan S, 2001, MOL BIOL EVOL, V18, P691, DOI 10.1093/oxfordjournals.molbev.a003851; Wyman SK, 2004, BIOINFORMATICS, V20, P3252, DOI 10.1093/bioinformatics/bth352; Yotsukura N, 2010, J APPL PHYCOL, V22, P243, DOI 10.1007/s10811-009-9452-7; Zhang J, 2013, J APPL PHYCOL, V25, P1247, DOI 10.1007/s10811-012-9915-0	42	7	7	0	13	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0921-8971	1573-5176		J APPL PHYCOL	J. Appl. Phycol.	APR	2015	27	2					1021	1028		10.1007/s10811-014-0386-3			8	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	CF3KA	WOS:000352446200039					2021-04-07	
J	Saez, CA; Gonzalez, A; Contreras, RA; Moody, AJ; Moenne, A; Brown, MT				Saez, Claudio A.; Gonzalez, Alberto; Contreras, Rodrigo A.; Moody, A. John; Moenne, Alejandra; Brown, Murray T.			A novel field transplantation technique reveals intra-specific metal-induced oxidative responses in strains of Ectocarpus siiiculosus with different pollution histories	ENVIRONMENTAL POLLUTION			English	Article						Brown algae; Metal accumulation; Antioxidant metabolism; Active biomonitoring	SCYTOSIPHON-LOMENTARIA PHAEOPHYCEAE; FUCUS-VESICULOSUS PHAEOPHYCEAE; ULVA-COMPRESSA CHLOROPHYTA; ASCORBATE PEROXIDASE; HYDROGEN-PEROXIDE; SUPEROXIDE-DISMUTASE; ANTIOXIDANT RESPONSES; MINING CONTAMINATION; COPPER ACCUMULATION; BROWN-ALGAE	A novel field transplantation technique, in which seaweed material is incorporated into dialysis tubing, was used to investigate intra-specific responses to metals in the model brown alga Ectocarpus siliculosus. Metal accumulation in the two strains was similar, with higher concentrations in material deployed to the metal-contaminated site (Ventanas, Chile) than the pristine site (Quintay, Chile). However, the oxidative responses differed. At Ventanas, strain Es147 (from low-polluted site) underwent oxidative damage whereas Es524 (from highly polluted site) was not affected. Concentrations of reduced ascorbate (ASC) and reduced glutathione (GSH) were significantly higher in Es524. Activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR) all increased in Es524, whereas only SOD increased in Es147. For the first time, employing a field transplantation technique, we provide unambiguous evidence of inter-population variation of metal-tolerance in brown algae and establish that antioxidant defences are, in part, responsible. (C) 2015 Elsevier Ltd. All rights reserved.	[Saez, Claudio A.; Brown, Murray T.] Univ Plymouth, Fac Sci & Environm, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England; [Saez, Claudio A.] Univ Playa Ancha, Fac Ingn, Dept Medio Ambiente, Valparaiso, Chile; [Saez, Claudio A.] Univ Playa Artcha, Ctr Estudios Avanzados, Vina Del Mar, Chile; [Gonzalez, Alberto; Contreras, Rodrigo A.; Moenne, Alejandra] Univ Santiago Chile, Fac Quim & Biol, Dept Biol, Santiago, Chile; [Moody, A. John] Univ Plymouth, Fac Sci & Environm, Sch Biol Sci, Plymouth PL4 8AA, Devon, England	Brown, MT (corresponding author), Univ Plymouth, Fac Sci & Environm, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England.	MTBrown@plymouth.ac.uk	Saez, Claudio/F-5978-2015; Figueroa, Alberto Gonzalez/AAA-7911-2020; Brown, Murray/K-5291-2014	Saez, Claudio/0000-0002-5037-3484; Figueroa, Alberto Gonzalez/0000-0002-5777-9022; Brown, Murray/0000-0003-2655-8611; Moody, John/0000-0002-1994-7419; Contreras, Rodrigo/0000-0001-9970-3125	Santander Postgraduate Mobility Support Scholarship; CONICYT Becas Chile Scholarship [72110557]	This study was funded by the Santander Postgraduate Mobility Support Scholarship 2011-12, awarded at Plymouth University to C. A. Saez. We thank financial support for PhD studies to C. A. Saez from CONICYT Becas Chile Scholarship (72110557). We also acknowledge Chita Guisado and Roberto Maltrain at Universidad de Valparaiso for providing laboratory facilities for culturing E. siliculosus and the necessary materials for the transplantation experiments.	Aebi H, 1984, METHOD ENZYMOL, V6, P105, DOI DOI 10.1016/S0076-6879(84)05016-3; Aiken CM, 2008, CONT SHELF RES, V28, P2371, DOI 10.1016/j.csr.2008.05.006; ASADA K, 1992, PHYSIOL PLANTARUM, V85, P235, DOI 10.1111/j.1399-3054.1992.tb04728.x; Benzie IFF, 1999, METHOD ENZYMOL, V299, P15; Brown M.T., 1998, METABOLISM TRACE MET, P185; Brown MT, 2012, ECOTOXICOLOGY, V21, P591, DOI 10.1007/s10646-011-0819-6; CHAPHEKAR SB, 1991, J ENVIRON BIOL, V12, P163; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Collen J, 2001, J PHYCOL, V37, P474, DOI 10.1046/j.1529-8817.2001.037004474.x; Connan S, 2011, AQUAT TOXICOL, V104, P1, DOI 10.1016/j.aquatox.2011.03.016; Contreras L, 2005, J PHYCOL, V41, P1184, DOI 10.1111/j.1529-8817.2005.00151.x; Contreras L, 2009, AQUAT TOXICOL, V94, P94, DOI 10.1016/j.aquatox.2009.06.004; De Philippis R, 2003, J APPL PHYCOL, V15, P155, DOI 10.1023/A:1023889410912; EVSTIGNEEV VB, 1974, BIOKHIMIYA, V39, P394; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; Gonzalez I, 2008, REV CHIL HIST NAT, V81, P279; HEATH RL, 1968, ARCH BIOCHEM BIOPHYS, V125, P189, DOI 10.1016/0003-9861(68)90654-1; Hedouin L, 2008, MAR ENVIRON RES, V66, P438, DOI 10.1016/j.marenvres.2008.07.005; Heinrich S., 2012, PLOS ONE, V7; Ken CF, 2005, J AGR FOOD CHEM, V53, P1470, DOI 10.1021/jf048269f; Kupper H, 2002, J PHYCOL, V38, P429, DOI 10.1046/j.1529-8817.2002.01148.x; KUTHAN H, 1986, BIOCHEM J, V237, P175, DOI 10.1042/bj2370175; Masashi H., 2007, BENEFICIAL HLTH EFFE, P297; Mellado M, 2012, PLANT PHYSIOL BIOCH, V51, P102, DOI 10.1016/j.plaphy.2011.10.007; Mhamdi A, 2010, PLANT PHYSIOL, V153, P1144, DOI 10.1104/pp.110.153767; MISHRA NP, 1993, PLANT PHYSIOL, V102, P903, DOI 10.1104/pp.102.3.903; Mizuno M, 1998, BIOCHEM MOL BIOL INT, V44, P717; NAKANO Y, 1981, PLANT CELL PHYSIOL, V22, P867; Neaman A, 2009, GEODERMA, V150, P359, DOI 10.1016/j.geoderma.2009.02.017; Nielsen HD, 2003, NEW PHYTOL, V160, P157, DOI 10.1046/j.1469-8137.2003.00864.x; Noctor G, 1998, ANNU REV PLANT PHYS, V49, P249, DOI 10.1146/annurev.arplant.49.1.249; Noctor G, 2012, PLANT CELL ENVIRON, V35, P454, DOI 10.1111/j.1365-3040.2011.02400.x; Patsikka E, 2002, PLANT PHYSIOL, V129, P1359, DOI 10.1104/pp.004788; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; PROVASOLI L, 1974, ALGAL PHYSL BIOCH, P741; Queval G, 2007, ANAL BIOCHEM, V363, P58, DOI 10.1016/j.ab.2007.01.005; Ratkevicius N, 2003, PLANT CELL ENVIRON, V26, P1599, DOI 10.1046/j.1365-3040.2003.01073.x; Rijstenbil JW, 2000, MAR BIOL, V137, P763, DOI 10.1007/s002270000443; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Saez CA, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0050170; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; Schutzendubel A, 2002, J EXP BOT, V53, P1351, DOI 10.1093/jexbot/53.372.1351; SEELY GR, 1972, MAR BIOL, V12, P184, DOI 10.1007/BF00350754; SENGUPTA A, 1993, PLANT PHYSIOL, V103, P1067, DOI 10.1104/pp.103.4.1067; Sergiev I, 1997, P B ACAD SCI, V51, P121; Serisawa Yukihiko, 2002, Phycological Research, V50, P201, DOI 10.1111/j.1440-1835.2002.tb00152.x; Sinnott ML, 2007, CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY: STRUCTURE AND MECHANISM, pV; Torres MA, 2008, ECOTOX ENVIRON SAFE, V71, P1, DOI 10.1016/j.ecoenv.2008.05.009; VANALSTYNE KL, 1995, J CHEM ECOL, V21, P45, DOI 10.1007/BF02033661	52	13	13	0	18	ELSEVIER SCI LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND	0269-7491	1873-6424		ENVIRON POLLUT	Environ. Pollut.	APR	2015	199						130	138		10.1016/j.envpol.2015.01.026			9	Environmental Sciences	Environmental Sciences & Ecology	CE6UE	WOS:000351972900017	25645062				2021-04-07	
J	Garcia-Jimenez, P; Robaina, RR				Garcia-Jimenez, Pilar; Robaina, Rafael R.			On reproduction in red algae: further research needed at the molecular level	FRONTIERS IN PLANT SCIENCE			English	Article						Rhodophyta; seaweeds; reproduction; light; hormones; photoreceptors; signaling	LEMANEIFORMIS GRACILARIALES; ECTOCARPUS-SILICULOSUS; CHONDRUS-CRISPUS; MODEL ORGANISM; LIFE-HISTORY; PLANT-GROWTH; IN-VITRO; RHODOPHYTA; LIGHT; PORPHYRA	Multicellular red algae(Rhodophyta) have some of the most complex life cycles known in living organisms. Economically valuable seaweeds, such as phycocolloid producers, have a triphasic(gametophyte, carposporophyte, and tetrasporophyte) life cycle, not to mention the intricate alternation of generations in the edible "sushi-alga" nori. It is a well-known fact that reproductive processes are controlled by one or more abiotic factor(s), including day length, light quality, temperature, and nutrients. Likewise, endogenous chemical factors such as plant growth regulators have been reported to affect reproductive events in some red seaweeds. Still, in the genomic era and given the high throughput techniques at our disposal, our knowledge about the endogenous molecular machinery lags far behind that of higher plants. Any potential effective control of there productive process will entail revisiting most of these results and facts to answer basic biological questions as yet unresolved. Recent results have shed light on the involvement of several genes in red alga reproductive events. In addition, a working species characterized by a simple filamentous architecture, easy cultivation, and accessible genomes may also facilitate our task.	[Garcia-Jimenez, Pilar; Robaina, Rafael R.] Univ Las Palmas Gran Canaria, Dept Biol, Las Palmas Gran Canaria 35017, Canary Islands, Spain	Robaina, RR (corresponding author), Univ Las Palmas Gran Canaria, Dept Biol, Las Palmas Gran Canaria 35017, Canary Islands, Spain.	rafael.robaina@ulpgc.es	Garcia-Jimenez, Pilar/K-2700-2019	Garcia-Jimenez, Pilar/0000-0002-4732-0381; Robaina, Rafael/0000-0003-4265-5809	Spanish Ministerio de Economia y Competitividad [BFU2003-01244, BFU2006-06918, BFU2010-17248]	The authors want to thank the support obtained from the Spanish Ministerio de Economia y Competitividad (Plan Nacional, grants # BFU2003-01244; 2006-06918; 2010-17248).	Agrawal SC, 2012, FOLIA MICROBIOL, V57, P387, DOI 10.1007/s12223-012-0147-0; Alabadi D, 2009, PLANT MOL BIOL, V69, P409, DOI 10.1007/s11103-008-9400-y; Andres F, 2012, NAT REV GENET, V13, P627, DOI 10.1038/nrg3291; Arun A, 2013, NEW PHYTOL, V197, P503, DOI 10.1111/nph.12007; Baweja P, 2009, PHYCOL RES, V57, P45, DOI 10.1111/j.1440-1835.2008.00520.x; Bornette G, 2011, AQUAT SCI, V73, P1, DOI 10.1007/s00027-010-0162-7; Chan CX, 2012, J PHYCOL, V48, P1328, DOI 10.1111/j.1529-8817.2012.01229.x; Chan CX, 2006, TRENDS PLANT SCI, V11, P165, DOI 10.1016/j.tplants.2006.02.003; Chen P, 2011, AFR J MICROBIOL RES, V5, P2590; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Cole K. M., 1995, BIOL RED SEAWEEDS; Collen J, 2014, ADV BOT RES, V71, P53, DOI 10.1016/B978-0-12-408062-1.00003-2; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; DREW KM, 1949, NATURE, V164, P748, DOI 10.1038/164748a0; DREW KM, 1954, NATURE, V173, P1243, DOI 10.1038/1731243a0; DRING MJ, 1988, ANNU REV PLANT PHYS, V39, P157, DOI 10.1146/annurev.pp.39.060188.001105; Garcia-Jimenez P, 1998, J APPL PHYCOL, V10, P95, DOI 10.1023/A:1008063532233; Garcia-Jimenez P, 2012, J PHYCOL, V48, P710, DOI 10.1111/j.1529-8817.2012.01156.x; Garcia-Jimenez P, 2009, J PLANT PHYSIOL, V166, P1745, DOI 10.1016/j.jplph.2009.04.018; Gupta N, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-38; Guzman-Uriostegui A, 2002, J PHYCOL, V38, P1169, DOI 10.1046/j.1529-8817.2002.01202.x; Guzman-Uriostegui A, 2012, BIOTECHNOL LETT, V34, P755, DOI 10.1007/s10529-011-0823-1; Gyula N, 2003, CURR OPIN PLANT BIOL, V6, P446, DOI 10.1016/S1369-5266(03)00082-7; Hohm T, 2013, AM J BOT, V100, P47, DOI 10.3732/ajb.1200299; Huang KY, 2002, PHYSIOL PLANTARUM, V115, P613, DOI 10.1034/j.1399-3054.2002.1150416.x; Jaillais Y, 2012, NAT CELL BIOL, V14, P788, DOI 10.1038/ncb2551; Kamiya M, 2011, EUR J PHYCOL, V46, P27, DOI 10.1080/09670262.2010.548101; Kianianmomeni A, 2014, PLANTA, V239, P1, DOI 10.1007/s00425-013-1962-5; Lau OS, 2010, CURR OPIN PLANT BIOL, V13, P571, DOI 10.1016/j.pbi.2010.07.001; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Lee YK, 1998, J PHYCOL, V34, P1017, DOI 10.1046/j.1529-8817.1998.341017.x; Leivar P, 2011, TRENDS PLANT SCI, V16, P19, DOI 10.1016/j.tplants.2010.08.003; Liu QY, 1996, PLANT MOL BIOL, V31, P77, DOI 10.1007/BF00020608; Liu YW, 2013, PLOS GENET, V9, DOI 10.1371/journal.pgen.1003861; Marian FD, 2000, PHYSIOL PLANTARUM, V110, P530, DOI 10.1111/j.1399-3054.2000.1100416.x; Mikami K, 2014, FRONT PLANT SCI, V5, DOI [10.3389/fpls.2014.00498, 10.3389/fpls.2014.00380]; Nakamura Y, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0057122; Onodera A, 2005, PLANT CELL PHYSIOL, V46, P367, DOI 10.1093/pcp/pci037; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Prochnik SE, 2010, SCIENCE, V329, P223, DOI 10.1126/science.1188800; Rebours C, 2014, J APPL PHYCOL, V26, P1939, DOI 10.1007/s10811-014-0304-8; Ren XY, 2009, J APPL PHYCOL, V21, P273, DOI 10.1007/s10811-008-9361-1; Sacramento AT, 2007, PLANT GROWTH REGUL, V53, P147, DOI 10.1007/s10725-007-9212-0; Sacramento AT, 2004, J PHYCOL, V40, P887, DOI 10.1111/j.1529-8817.2004.03183.x; Sahoo D, 2002, CURR SCI INDIA, V83, P1313; SHEVLIN DE, 1978, J PHYCOL, V14, P282, DOI 10.1111/j.1529-8817.1978.tb00300.x; Sutherland JE, 2011, J PHYCOL, V47, P1131, DOI 10.1111/j.1529-8817.2011.01052.x; Uji T, 2013, MAR BIOTECHNOL, V15, P188, DOI 10.1007/s10126-012-9475-y; Uji T, 2012, MOL BIOL REP, V39, P7973, DOI 10.1007/s11033-012-1643-7; Xu DQ, 2014, PLOS GENET, V10, DOI 10.1371/journal.pgen.1004197; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zhong SW, 2012, CURR BIOL, V22, P1530, DOI 10.1016/j.cub.2012.06.039	53	7	7	2	83	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-462X			FRONT PLANT SCI	Front. Plant Sci.	FEB 23	2015	6								93	10.3389/fpls.2015.00093			6	Plant Sciences	Plant Sciences	CB6VA	WOS:000349763600001	25755663	DOAJ Gold, Green Published			2021-04-07	
J	Billoud, B; Jouanno, E; Nehr, Z; Carton, B; Rolland, E; Chenivesse, S; Charrier, B				Billoud, Bernard; Jouanno, Emilie; Nehr, Zofia; Carton, Baptiste; Rolland, Elodie; Chenivesse, Sabine; Charrier, Benedicte			Localization of causal locus in the genome of the brown macroalga Ectocarpus: NGS-based mapping and positional cloning approaches	FRONTIERS IN PLANT SCIENCE			English	Article						macroalga; mutant; genetic locus; positional cloning; NGS-based mapping; SNP mapping; SSR	IDENTIFICATION; ARABIDOPSIS; MUTATIONS; PHAEOPHYCEAE; POLYMORPHISM; SILICULOSUS; ACCESSION; GENETICS; SYSTEM; MODEL	Mutagenesis is the only process by which unpredicted biological gene function can be identified. Despite that several macroalgal developmental mutants have been generated, their causal mutation was never identified, because experimental conditions were not gathered at that time. Today, progresses in macroalgal genomics and judicious choices of suitable genetic models make mutated gene identification possible. This article presents a comparative study of two methods aiming at identifying a genetic locus in the brown alga Ectocarpus siliculosus: positional cloning and Next-Generation Sequencing (NGS)-based mapping. Once necessary preliminary experimental tools were gathered, we tested both analyses on an Ectocarpus morphogenetic mutant. We show how a narrower localization results from the combination of the two methods. Advantages and drawbacks of these two approaches as well as potential transfer to other macroalgae are discussed.	[Billoud, Bernard; Jouanno, Emilie; Nehr, Zofia; Carton, Baptiste; Rolland, Elodie; Chenivesse, Sabine; Charrier, Benedicte] Univ Paris 06, Stn Biol Roscoff, UMR 8227, Sorbonne Univ,CNRS, F-29688 Roscoff, France	Billoud, B (corresponding author), Stn Biol Roscoff, UMR 8227, Pl Georges Tessier, F-29680 Roscoff, France.	bernard.billoud@sb-roscoff.fr		Charrier, Benedicte/0000-0001-5721-1640; Billoud, Bernard/0000-0002-5140-8087			Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Austin RS, 2011, PLANT J, V67, P715, DOI 10.1111/j.1365-313X.2011.04619.x; Balata D, 2011, MAR BIOL, V158, P2459, DOI 10.1007/s00227-011-1747-y; Billoud B, 2008, FUNCT PLANT BIOL, V35, P1014, DOI 10.1071/FP08036; Bowen ME, 2012, GENETICS, V190, P1017, DOI 10.1534/genetics.111.136069; Camacho C, 2009, BMC BIOINFORMATICS, V10, DOI 10.1186/1471-2105-10-421; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Clark MD, 2011, METHOD CELL BIOL, V104, P221, DOI 10.1016/B978-0-12-374814-0.00013-6; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Delaroque N, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-110; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Fritsch F.E., 1945, STRUCTURE REPROD ALG; Gebhardt C, 2005, INT REV CYTOL, V247, P223, DOI 10.1016/S0074-7696(05)47005-9; HANISAK MD, 1988, MAR BIOL, V99, P157, DOI 10.1007/BF00391977; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Jander G, 2002, PLANT PHYSIOL, V129, P440, DOI 10.1104/pp.003533; Joshi NA, 2011, SICKLE ASLIDING WIND; Kendal WS, 2003, MOL BIOL EVOL, V20, P579, DOI 10.1093/molbev/msg057; Knight M., 1930, EARTH ENV SCI T R SO, V56, P307, DOI [10.1017/S0080456800013223, DOI 10.1017/S0080456800013223]; Laitinen RAE, 2010, PLANT PHYSIOL, V153, P652, DOI 10.1104/pp.110.156448; Le Bail Aude, 2013, Methods Mol Biol, V959, P323, DOI 10.1007/978-1-62703-221-6_22; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Lee J, 2013, GENES GENOM, V35, P415, DOI 10.1007/s13258-013-0115-3; LITTLER MM, 1983, J PHYCOL, V19, P229, DOI 10.1111/j.0022-3646.1983.00229.x; Luck J, 1999, ACTA BIOTHEOR, V47, P329, DOI 10.1023/A:1002659209511; Lukowitz W, 2000, PLANT PHYSIOL, V123, P795, DOI 10.1104/pp.123.3.795; Martins WS, 2009, BIOINFORMATION, V3, P282, DOI 10.6026/97320630003282; Matsubara K, 2014, FRONT PLANT SCI, V5, DOI 10.3389/fpls.2014.00193; MICHELMORE RW, 1991, P NATL ACAD SCI USA, V88, P9828, DOI 10.1073/pnas.88.21.9828; Mikami K, 2014, FRONT PLANT SCI, V5, DOI [10.3389/fpls.2014.00498, 10.3389/fpls.2014.00380]; Mueller UG, 1999, TRENDS ECOL EVOL, V14, P389, DOI 10.1016/S0169-5347(99)01659-6; Muller DG, 1975, LYNGB ARCH PROTISTEN, V117, P297; Peterlongo P, 2010, LECT NOTES COMPUT SC, V6393, P147, DOI 10.1007/978-3-642-16321-0_14; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Quarrie SA, 1996, PLANT GROWTH REGUL, V20, P167, DOI 10.1007/BF00024013; Schneeberger K, 2009, NAT METHODS, V6, P550, DOI 10.1038/nmeth0809-550; Schuelke M, 2000, NAT BIOTECHNOL, V18, P233, DOI 10.1038/72708; STENECK RS, 1994, OIKOS, V69, P476, DOI 10.2307/3545860; Sturtevant AH, 1913, J EXP ZOOL, V14, P43, DOI 10.1002/jez.1400140104; Tabata R, 2013, PLANT SIGNAL BEHAV, V8, DOI 10.4161/psb.22534; Uchida N, 2011, PLANT CELL PHYSIOL, V52, P716, DOI 10.1093/pcp/pcr029; Vollbrecht E, 2005, BIOCHEM SOC T, V33, P1502, DOI 10.1042/BST20051502; Zuryn S, 2010, GENETICS, V186, P427, DOI 10.1534/genetics.110.119230	47	4	5	0	17	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-462X			FRONT PLANT SCI	Front. Plant Sci.	FEB 19	2015	6								68	10.3389/fpls.2015.00068			12	Plant Sciences	Plant Sciences	CB5CP	WOS:000349645400001	25745426	DOAJ Gold, Green Published			2021-04-07	
J	Torode, TA; Marcus, SE; Jam, M; Tonon, T; Blackburn, RS; Herve, C; Knox, JP				Torode, Thomas A.; Marcus, Susan E.; Jam, Murielle; Tonon, Thierry; Blackburn, Richard S.; Herve, Cecile; Knox, J. Paul			Monoclonal Antibodies Directed to Fucoidan Preparations from Brown Algae	PLOS ONE			English	Article							PLANT-CELL WALLS; FUCUS-SERRATUS; SULFATED POLYSACCHARIDES; LAMPROTHAMNIUM-PAPULOSUM; PECTIC HOMOGALACTURONAN; ECTOCARPUS-SILICULOSUS; EXTRACELLULAR-MATRIX; EVOLUTION; HETEROGENEITY; PHAEOPHYCEAE	Cell walls of the brown algae contain a diverse range of polysaccharides with useful bioactivities. The precise structures of the sulfated fucan/fucoidan group of polysaccharides and their roles in generating cell wall architectures and cell properties are not known in detail. Four rat monoclonal antibodies, BAM1 to BAM4, directed to sulfated fucan preparations, have been generated and used to dissect the heterogeneity of brown algal cell wall polysaccharides. BAM1 and BAM4, respectively, bind to a non-sulfated epitope and a sulfated epitope present in the sulfated fucan preparations. BAM2 and BAM3 identified additional distinct epitopes present in the fucoidan preparations. All four epitopes, not yet fully characterised, occur widely within the major brown algal taxonomic groups and show divergent distribution patterns in tissues. The analysis of cell wall extractions and fluorescence imaging reveal differences in the occurrence of the BAM1 to BAM4 epitopes in various tissues of Fucus vesiculosus. In Ectocarpus subulatus, a species closely related to the brown algal model Ectocarpus siliculosus, the BAM4 sulfated epitope was modulated in relation to salinity levels. This new set of monoclonal antibodies will be useful for the dissection of the highly complex and yet poorly resolved sulfated polysaccharides in the brown algae in relation to their ecological and economic significance.	[Torode, Thomas A.; Marcus, Susan E.; Knox, J. Paul] Univ Leeds, Ctr Plant Sci, Fac Biol Sci, Leeds, W Yorkshire, England; [Jam, Murielle; Tonon, Thierry; Herve, Cecile] Sorbonne Univ, Univ Paris 06, UMR 8227, Stn Biol Roscoff, F-90074 Roscoff, France; [Jam, Murielle; Tonon, Thierry; Herve, Cecile] CNRS, Stn Biol Roscoff, UMR 8227, Roscoff, France; [Blackburn, Richard S.] Univ Leeds, Ctr Tech Text, Sustainable Mat Res Grp, Leeds, W Yorkshire, England	Knox, JP (corresponding author), Univ Leeds, Ctr Plant Sci, Fac Biol Sci, Leeds, W Yorkshire, England.	j.p.knox@leeds.ac.uk	Knox, Paul/H-4577-2012; Tonon, Thierry/A-3214-2009; Blackburn, Richard Simon/B-3210-2016	Knox, Paul/0000-0002-9231-6891; Tonon, Thierry/0000-0002-1454-6018; Blackburn, Richard Simon/0000-0001-6259-3807; Torode, Thomas/0000-0001-9717-6923; Herve, Cecile/0000-0001-6649-8137	UK Biotechnology and Biological Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC); Assemble [227799]; European Union FP7 research network; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [977478] Funding Source: researchfish	This work was supported by a UK Biotechnology and Biological Research Council (http://www.bbsrc.ac.uk) studentship to TAT. TAT also received two awards from Assemble (#227799), a European Union FP7 research network (http://www.assemblemarine.org/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Ale MT, 2011, MAR DRUGS, V9, P2106, DOI 10.3390/md9102106; Anastyuk SD, 2010, CARBOHYD RES, V345, P2206, DOI 10.1016/j.carres.2010.07.043; Aquino RS, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0018862; Bilan MI, 2010, CARBOHYD RES, V345, P2038, DOI 10.1016/j.carres.2010.07.009; Brownlee C, 2001, SEMIN CELL DEV BIOL, V12, P345, DOI 10.1006/scdb.2001.0262; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Cornuault V, 2014, PLANT J, V78, P715, DOI 10.1111/tpj.12504; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Descamps V, 2006, MAR BIOTECHNOL, V8, P27, DOI 10.1007/s10126-005-5107-0; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; EARDLEY DD, 1990, J PHYCOL, V26, P54, DOI 10.1111/j.0022-3646.1990.00054.x; Enquist-Newman M, 2014, NATURE, V505, P239, DOI 10.1038/nature12771; GREEN JR, 1993, NEW PHYTOL, V124, P397, DOI 10.1111/j.1469-8137.1993.tb03830.x; Hahn T, 2012, PROCESS BIOCHEM, V47, P1691, DOI 10.1016/j.procbio.2012.06.016; JONES JL, 1988, PLANTA, V176, P298, DOI 10.1007/BF00395410; KLEIN MD, 1982, ANAL BIOCHEM, V124, P59, DOI 10.1016/0003-2697(82)90219-6; KLOAREG B, 1986, INT J BIOL MACROMOL, V8, P380, DOI 10.1016/0141-8130(86)90060-7; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Kolender AA, 2004, CARBOHYD RES, V339, P1619, DOI 10.1016/j.carres.2004.03.029; Kovalenko I, 2011, SCIENCE, V334, P75, DOI 10.1126/science.1209150; LARSEN B, 1985, CARBOHYD RES, V143, P221, DOI 10.1016/S0008-6215(00)90710-9; LARSEN B, 1966, ACTA CHEM SCAND, V20, P219, DOI 10.3891/acta.chem.scand.20-0219; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Lee KJD, 2013, PLANT J, V75, P1018, DOI 10.1111/tpj.12263; Lees A, 1996, VACCINE, V14, P190, DOI 10.1016/0264-410X(95)00195-7; MABEAU S, 1987, J EXP BOT, V38, P1573, DOI 10.1093/jxb/38.9.1573; Manabe Y, 2011, PLANT PHYSIOL, V155, P1068, DOI 10.1104/pp.110.168989; Marcus SE, 2008, BMC PLANT BIOL, V8, DOI 10.1186/1471-2229-8-60; MARIANI P, 1985, PROTOPLASMA, V128, P208, DOI 10.1007/BF01276343; McCartney L, 2005, J HISTOCHEM CYTOCHEM, V53, P543, DOI 10.1369/jhc.4B6578.2005; MCCULLY ME, 1968, PROTOPLASMA, V66, P205, DOI 10.1007/BF01252533; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; PARSONS TF, 1980, P NATL ACAD SCI-BIOL, V77, P7089, DOI 10.1073/pnas.77.12.7089; Pedersen HL, 2012, J BIOL CHEM, V287, DOI 10.1074/jbc.M112.396598; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Pomin VH, 2008, GLYCOBIOLOGY, V18, P1016, DOI 10.1093/glycob/cwn085; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Seaweeds TD, 2002, NATURAL HIST MUSEUM; Senni K, 2011, MAR DRUGS, V9, P1664, DOI 10.3390/md9091664; Shepherd VA, 1999, J MEMBRANE BIOL, V170, P229, DOI 10.1007/s002329900552; Shepherd VA, 1999, PLANT CELL ENVIRON, V22, P333, DOI 10.1046/j.1365-3040.1999.00414.x; VREELAND V, 1984, PLANTA, V162, P506, DOI 10.1007/BF00399916; Wargacki AJ, 2012, SCIENCE, V335, P308, DOI 10.1126/science.1214547; Wei N, 2013, TRENDS BIOTECHNOL, V31, P70, DOI 10.1016/j.tibtech.2012.10.009; West John A., 1996, Muelleria, V9, P29; Willats WGT, 1998, CARBOHYD RES, V308, P149, DOI 10.1016/S0008-6215(98)00070-6; Willats WGT, 2001, J BIOL CHEM, V276, P19404, DOI 10.1074/jbc.M011242200; WOODWARD MP, 1985, J IMMUNOL METHODS, V78, P143, DOI 10.1016/0022-1759(85)90337-0; Zhao D, 2012, MOL BIOSYST, V8, P753, DOI 10.1039/c1mb05337j	52	28	28	2	38	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	FEB 18	2015	10	2							e0118366	10.1371/journal.pone.0118366			19	Multidisciplinary Sciences	Science & Technology - Other Topics	CC0WZ	WOS:000350061500119	25692870	DOAJ Gold, Green Published, Green Accepted			2021-04-07	
J	Saint-Marcoux, D; Billoud, B; Langdale, JA; Charrier, B				Saint-Marcoux, Denis; Billoud, Bernard; Langdale, Jane A.; Charrier, Benedicte			Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae	FRONTIERS IN PLANT SCIENCE			English	Article						laser capture microdissection; cell-specific transcriptomics; cell differentiation; seaweed; brown algae	GENE-EXPRESSION; RNA-SEQ; AMPLIFICATION; EVOLUTION; GENOME; LONG; TOOL	Laser capture microdissection (LCM) facilitates the isolation of individual cells from tissue sections, and when combined with RNA amplification techniques, it is an extremely powerful tool for examining genome-wide expression profiles in specific cell-types. LCM has been widely used to address various biological questions in both animal and plant systems, however, no attempt has been made so far to transfer LCM technology to macroalgae. Macroalgae are a collection of widespread eukaryotes living in fresh and marine water. In line with the collective effort to promote molecular investigations of macroalgal biology, here we demonstrate the feasibility of using LCM and cell-specific transcriptomics to study development of the brown alga Ectocarpus siliculosus. We describe a workflow comprising cultivation and fixation of algae on glass slides, laser microdissection, and RNA amplification. To illustrate the effectiveness of the procedure, we show qPCR data and metrics obtained from cell-specific transcriptomes generated from both upright and prostrate filaments of Ectocarpus.	[Saint-Marcoux, Denis; Langdale, Jane A.] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England; [Billoud, Bernard; Charrier, Benedicte] Univ Paris 06, Sorbonne Univ, CNRS, Stn Biol Roscoff,UMR 8227, Roscoff, France	Saint-Marcoux, D (corresponding author), Univ Oxford, Dept Plant Sci, S Parks Rd, Oxford OX1 3RB, England.	denis.saint-marcoux@plants.ox.ac.uk	Langdale, Jane A./AAE-8343-2019	Langdale, Jane/0000-0001-7648-3924; Billoud, Bernard/0000-0002-5140-8087; Charrier, Benedicte/0000-0001-5721-1640	ERC Advanced Investigator Grant (EDIP); Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [G19201] Funding Source: researchfish	This work was funded by an ERC Advanced Investigator Grant (EDIP) to Jane A. Langdale.	Adiconis X, 2013, NAT METHODS, V10, P623, DOI 10.1038/nmeth.2483; ANDERS S, 2010, GENOME BIOL, V11, DOI DOI 10.1186/GB-2010-11-10-R106; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Bhargava V, 2014, SCI REP-UK, V4, DOI 10.1038/srep03678; Billoud B, 2015, FRONT PLANT SCI, V6, DOI 10.3389/fpls.2015.00068; Boelens MC, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-277; Brandt S, 1999, PLANT J, V20, P245, DOI 10.1046/j.1365-313x.1999.00583.x; Brooks L, 2009, PLOS GENET, V5, DOI 10.1371/journal.pgen.1000476; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Clark MB, 2011, PLOS BIOL, V9, DOI 10.1371/journal.pbio.1000625; Clement-Ziza M, 2009, BMC GENOMICS, V10, DOI 10.1186/1471-2164-10-246; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; EmmertBuck MR, 1996, SCIENCE, V274, P998, DOI 10.1126/science.274.5289.998; Espina V, 2006, NAT PROTOC, V1, P586, DOI 10.1038/nprot.2006.85; Fatica A, 2014, NAT REV GENET, V15, P7, DOI 10.1038/nrg3606; Fosu-Nyarko J, 2010, METHODS MOL BIOL, V638, P153, DOI 10.1007/978-1-60761-611-5_11; Fritsch F.E., 1945, STRUCTURE REPROD ALG; Gandotra N, 2013, PLANT J, V74, P48, DOI 10.1111/tpj.12100; Ghildiyal M, 2009, NAT REV GENET, V10, P94, DOI 10.1038/nrg2504; Goldsworthy SM, 1999, MOL CARCINOGEN, V25, P86, DOI 10.1002/(SICI)1098-2744(199906)25:2<86::AID-MC2>3.3.CO;2-W; Gomez SK, 2009, PEST MANAG SCI, V65, P504, DOI 10.1002/ps.1715; He D, 2014, CURR BIOL, V24, P465, DOI 10.1016/j.cub.2014.01.036; Head SR, 2011, BIOTECHNIQUES, V50, P177, DOI 10.2144/000113613; Hollender CA, 2014, PLANT PHYSIOL, V165, P1062, DOI 10.1104/pp.114.237529; Jacquier A, 2009, NAT REV GENET, V10, P833, DOI 10.1038/nrg2683; Joosen R, 2007, PLANT PHYSIOL, V144, P155, DOI 10.1104/pp.107.098723; Joshi N.A., 2011, SICKLE SLIDING WINDO; KARRER EE, 1995, P NATL ACAD SCI USA, V92, P3814, DOI 10.1073/pnas.92.9.3814; Kim D, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-4-r36; Kopylova E, 2012, BIOINFORMATICS, V28, P3211, DOI 10.1093/bioinformatics/bts611; Kurn N, 2005, CLIN CHEM, V51, P1973, DOI 10.1373/clinchem.2005.053694; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail Aude, 2013, Methods Mol Biol, V959, P323, DOI 10.1007/978-1-62703-221-6_22; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Leliaert F, 2012, CRIT REV PLANT SCI, V31, P1, DOI 10.1080/07352689.2011.615705; Mercer TR, 2009, NAT REV GENET, V10, P155, DOI 10.1038/nrg2521; Nakamura Y, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0057122; Nakazono M, 2003, PLANT CELL, V15, P583, DOI 10.1105/tpc.008102; Nehr Z, 2011, PLANT SIGNAL BEHAV, V6, P1889, DOI 10.4161/psb.6.12.18054; Ogo Y, 2014, NEW PHYTOL, V201, P781, DOI 10.1111/nph.12577; Phillips Jennifer, 1996, Methods (Orlando), V10, P283, DOI 10.1006/meth.1996.0104; Robinson MD, 2010, BIOINFORMATICS, V26, P139, DOI 10.1093/bioinformatics/btp616; Ruzin SE., 1999, PLANT MICROTECHNIQUE; Schneider J, 2004, BMC GENOMICS, V5, DOI 10.1186/1471-2164-5-29; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Sun ZK, 2013, PLOS ONE, V8, DOI [10.1371/journal.pone.0055249, 10.1371/journal.pone.0055124]; Takahashi H, 2010, J PLANT RES, V123, P807, DOI 10.1007/s10265-010-0319-4; Tariq MA, 2011, NUCLEIC ACIDS RES, V39, DOI 10.1093/nar/gkr547; Teichert I, 2012, BMC GENOMICS, V13, DOI 10.1186/1471-2164-13-511; Trapnell C, 2009, BIOINFORMATICS, V25, P1105, DOI 10.1093/bioinformatics/btp120; Wang Z, 2009, NAT REV GENET, V10, P57, DOI 10.1038/nrg2484; Wu JQ, 2010, P NATL ACAD SCI USA, V107, P5254, DOI 10.1073/pnas.0914114107; Zhang X, 2007, PLOS GENET, V3, P1040, DOI 10.1371/journal.pgen.0030101	56	8	9	0	17	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-462X			FRONT PLANT SCI	Front. Plant Sci.	FEB 10	2015	6								54	10.3389/fpls.2015.00054			11	Plant Sciences	Plant Sciences	CA8ZM	WOS:000349209800001	25713580	DOAJ Gold, Green Published			2021-04-07	
J	Peters, AF; Couceiro, L; Tsiamis, K; Kupper, FC; Valero, M				Peters, Akira F.; Couceiro, Lucia; Tsiamis, Konstantinos; Kuepper, Frithjof C.; Valero, Myriam			Barcoding of cryptic stages of marine brown algae isolated from incubated substratum reveals high diversity in Acinetosporaceae (Ectocarpales, Phaeophyceae)	CRYPTOGAMIE ALGOLOGIE			English	Article						Acinetospora; biogeography; brown algae; cytochrome c oxidase 1; cox1; COI; cryptic stages; diversity; DNA barcoding; Ectocarpus subulatus Kutzing; Feldmannia; germling emergence; Hincksia; identification; phylogeny	LIFE-HISTORY; MOLECULAR PHYLOGENY; SP-NOV; SPECIES DELIMITATION; MITOCHONDRIAL GENOME; SYSTEMATIC POSITION; COMPLETE SEQUENCE; DNA; CULTURE; DICTYOSIPHONALES	To identify cryptic stages of marine brown macroalgae present in the "bank of microscopic forms", we incubated natural substrata of different geographical origins and isolated emerging Phaeophyceae into clonal cultures. A total of 431 clones were subsequently identified by barcoding using 5'-COI. A proportion of 98% of the isolates belonged to the Ectocarpales. The distribution of pairwise genetic distances revealed a K2P divergence of 1.8% as species-level cut-off. Using this threshold, the samples were ascribed to 83 different species, 39 (47%) of which were identified through reference sequences or morphology. In the Ectocarpaceae, 16 lineages of Ectocarpus fulfilled the barcode criterion for different species, while three putative new species were detected. In the Chordariaceae, numerous microthalli were microstages of known macroscopic taxa. A separate cluster contained Hecatonema maculans and other microscopic species. Taxa traditionally classified in Acinetosporaceae were split in two species-rich groups containing Pylaiella and Hincksia in one and Acinetospora in the other. Feldmannia species were present in both clusters. The present study shows that the germling emergence method is suited to reveal the diversity of hidden life-history stages, albeit with a bias towards early successional species.	[Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France; [Peters, Akira F.] CNRS, Biol Stn, FR2424, F-29682 Roscoff, France; [Couceiro, Lucia; Valero, Myriam] Sorbonne Univ UPMC, Evolutionary Biol & Ecol Algae, Stn Biol Roscoff, UMI EBEA 3614,CNRS, F-29688 Roscoff, France; [Tsiamis, Konstantinos] HCMR, Inst Oceanog, Anavyssos 19013, Attica, Greece; [Kuepper, Frithjof C.] Univ Aberdeen, Oceanlab, Newburgh AB41 6AA, Scotland	Peters, AF (corresponding author), Bezhin Rosko, F-29250 Santec, France.	akirapeters@gmail.com	Valero, Myriam/M-6052-2019; Valero, Myriam/C-7550-2011; Tsiamis, Konstantinos/N-7480-2014; Couceiro, Lucia/M-1851-2014	Valero, Myriam/0000-0002-9000-1423; Valero, Myriam/0000-0002-9000-1423; Tsiamis, Konstantinos/0000-0003-1192-3516; Couceiro, Lucia/0000-0003-4300-5744; Kuepper, Frithjof/0000-0003-1273-7109; Peters, Akira/0000-0001-5332-199X; Couceiro, Lucia/0000-0001-9466-8164	EU INTERREG programme France (Channel)-England; EUEuropean Commission [227788]; Agence Nationale de la Recherche (France)French National Research Agency (ANR) [ANR-2010-BLAN-1727]; IDEALG [ANR-10-BTBR-04-02]; "Bibliotheque du vivant" (France: INRA-MNHN-INEE-CNRS); TOTAL Foundation (Project "Brown algal biodiversity and ecology in the Eastern Mediterranean Sea"); Marine Biological Association (Plymouth, UK: Ray-Lankester fellowship to AFP); Marine Alliance for Science and Technology for Scotland pooling initiative; Scottish Funding Council [HR09011]	We wish to thank Dieter G. Muller, Hiroshi Kawai, Ergun Taskin and Eric C. Henry for help with identification of selected field material and cultures, Dieter G. Muller for five reference strains from his culture collection, Declan C. Schroeder for a raw isolate and Ga Youn Cho for information on the distribution of Korean kelps. Sampling in Italy greatly benefitted from the advice and logistic support by members of the Stazione Zoologica di Napoli. Our work was funded by the EU INTERREG programme France (Channel)-England (project MARINEXUS), the EU FP7 "capacities" specific programme ASSEMBLE (grant no. 227788), the Agence Nationale de la Recherche (France; projects BI-CYCLE ANR-2010-BLAN-1727 and IDEALG ANR-10-BTBR-04-02), the project "Bibliotheque du vivant" (France: INRA-MNHN-INEE-CNRS), the TOTAL Foundation (Project "Brown algal biodiversity and ecology in the Eastern Mediterranean Sea"), and the Marine Biological Association (Plymouth, UK: Ray-Lankester fellowship to AFP). This work also received support from the Marine Alliance for Science and Technology for Scotland pooling initiative. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions.	Ahmed S, 2014, CURR BIOL, V24, P1945, DOI 10.1016/j.cub.2014.07.042; Alongi G, 2007, PHYCOL RES, V55, P42, DOI 10.1111/j.1440-1835.2006.00445.x; Bittner L, 2008, MOL PHYLOGENET EVOL, V49, P211, DOI 10.1016/j.ympev.2008.06.018; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Burkhardt E, 1998, J PHYCOL, V34, P682, DOI 10.1046/j.1529-8817.1998.340682.x; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; CHAPMAN ARO, 1984, J EXP MAR BIOL ECOL, V78, P99, DOI 10.1016/0022-0981(84)90072-8; Chase MW, 2005, PHILOS T R SOC B, V360, P1889, DOI 10.1098/rstb.2005.1720; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; COLLINS F.S., 1896, B TORREY BOT CLUB, V23, P458; Cormaci M., 2012, B ACCADEMIA GIOENIA, V45, P1; DANGEARD P.J.L., 1970, LE BOT, V53, P23; Dayrat B, 2005, BIOL J LINN SOC, V85, P407, DOI 10.1111/j.1095-8312.2005.00503.x; DeSalle R, 2005, PHILOS T R SOC B, V360, P1905, DOI 10.1098/rstb.2005.1722; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; FELDMANN J, 1954, TRAV STAT BIOL ROS S, V6, P1; FELDMANN J., 1964, TRAVAUX STATION BI S, V15, P1; GEOFFROY A., 2015, J PHYCOLOGY IN PRESS; HAMEL G, 1939, PHEOPHYCEES FRANCE; HARVEY WH, 1846, PHYCOLOGIA BRITANNIC; HOFFMANN AJ, 1991, MAR ECOL PROG SER, V79, P185, DOI 10.3354/meps079185; Kawai H, 2007, J PHYCOL, V43, P186, DOI 10.1111/j.1529-8817.2006.00308.x; Kawase H, 2013, SCI REP-UK, V3, DOI 10.1038/srep02106; KIM H.-S., 2010, ALGAL FLORA OF KOREA, V2; Kim MS, 2010, CRYPTOGAMIE ALGOL, V31, P387; Kutzing F.T., 1843, PHYCOLOGIA GEN; Lane CE, 2007, MOL PHYLOGENET EVOL, V44, P634, DOI 10.1016/j.ympev.2007.03.016; Le Gall L, 2010, J PHYCOL, V46, P374, DOI 10.1111/j.1529-8817.2010.00807.x; LEBLANC C, 1995, J MOL BIOL, V250, P484, DOI 10.1006/jmbi.1995.0392; Leliaert F, 2014, EUR J PHYCOL, V49, P179, DOI 10.1080/09670262.2014.904524; LIM BL, 1986, JPN J GENET, V61, P169, DOI 10.1266/jjg.61.169; LOISEAUX S, 1969, PHYCOLOGIA, V8, P11; Loiseaux S., 1967, REV GEN BOT, V74, P529; Lotze HK, 2001, LIMNOL OCEANOGR, V46, P749, DOI 10.4319/lo.2001.46.4.0749; Maier I, 1998, EUR J PHYCOL, V33, P213, DOI 10.1017/S0967026298001747; Mattio L, 2010, CRYPTOGAMIE ALGOL, V31, P467; McDevit DC, 2009, PHYCOL RES, V57, P131, DOI 10.1111/j.1440-1835.2009.00530.x; MULLER DG, 1981, J PHYCOL, V17, P384, DOI 10.1111/j.0022-3646.1981.00384.x; MULLER DG, 1969, NATURWISSENSCHAFTEN, V56, P220; MULLER DG, 1994, BOT MAR, V37, P205, DOI 10.1515/botm.1994.37.3.205; MULLER DG, 1993, HYDROBIOLOGIA, V261, P37; Mystikou A, 2014, POLAR BIOL, V37, P1607, DOI 10.1007/s00300-014-1547-1; Oudot-le Secq MP, 2002, EUR J PHYCOL, V37, P163, DOI 10.1017/S0967026202003542; Oudot-Le Secq MP, 2001, J MOL EVOL, V53, P80, DOI 10.1007/s002390010196; PEDERSEN PM, 1989, NORD J BOT, V9, P443, DOI 10.1111/j.1756-1051.1989.tb01024.x; PETERS AF, 1988, BRIT PHYCOL J, V23, P299, DOI 10.1080/00071618800650331; PETERS AF, 1991, REV CHIL HIST NAT, V64, P261; PETERS AF, 1992, BRIT PHYCOL J, V27, P177, DOI 10.1080/00071619200650181; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; PETERS AF, 1991, PHYCOLOGIA, V30, P365, DOI 10.2216/i0031-8884-30-4-365.1; Peters AF, 1996, NOVA HEDWIGIA, V62, P341; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Peters AF, 1998, PHYCOLOGIA, V37, P114, DOI 10.2216/i0031-8884-37-2-114.1; Peters AF, 2003, P INT SEAW S, V17, P293; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; RAMIREZ ME, 1991, BOT MAR, V34, P133, DOI 10.1515/botm.1991.34.2.133; Roberts H. A., 1981, Advances in Applied Biology, V6, P1; Robuchon M, 2014, EUR J PHYCOL, V49, P128, DOI 10.1080/09670262.2014.892635; Rosenvinge L. K., 1893, MEDDELELSER GRONLAND, V3, P763; Saunders GW, 2013, BMC ECOL, V13, DOI 10.1186/1472-6785-13-9; Saunders Gary W, 2012, Methods Mol Biol, V858, P207, DOI 10.1007/978-1-61779-591-6_10; Saunders GW, 2005, PHILOS T R SOC B, V360, P1879, DOI 10.1098/rstb.2005.1719; Sauvageau C, 1913, CR HEBD ACAD SCI, V161, P796; SAUVAGEAU C., 1896, J BOT, V10, P388; SAUVAGEAU C., 1899, J BOT, V13, P107; SAUVAGEAU C., 1896, CR HEBD ACAD SCI, V123, P360; SAUVAGEAU C., 1933, B STATION BIOL ARCAC, V30, P80; SAUVAGEAU C., 1917, CR HEBD ACAD SCI, V164, P829; Sayer MDJ, 2013, DIVING HYPERB MED, V43, P239; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; Silberfeld T, 2011, EUR J PHYCOL, V46, P361, DOI 10.1080/09670262.2011.628698; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stern RF, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0013991; Tatewaki M, 1966, PHYCOLOGIA, V6, P62, DOI DOI 10.2216/I0031-8884-6-1-62.1; Tsiamis K, 2013, MEDITERR MAR SCI, V14, P141, DOI 10.12681/mms.315; Tsiamis K, 2017, J MAR BIOL ASSOC UK, V97, P681, DOI 10.1017/S0025315414000952; Tsiamis K, 2014, BOT MAR, V57, P153, DOI 10.1515/bot-2014-0006; West JA, 2012, CAH BIOL MAR, V53, P255; Yang EC, 2014, J PHYCOL, V50, P149, DOI 10.1111/jpy.12148; Yang W. X., 1993, SCI PAPERS I ALGOLOG, V9, P33; Zuccarello GC, 2011, J PHYCOL, V47, P627, DOI 10.1111/j.1529-8817.2011.00985.x	84	34	34	0	32	ADAC-CRYPTOGAMIE	PARIS	12 RUE DE BUFFON, 75005 PARIS, FRANCE	0181-1568	1776-0984		CRYPTOGAMIE ALGOL	Cryptogam. Algol.	FEB	2015	36	1					3	29		10.7872/crya.v36.iss1.2015.3			27	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	CD0OE	WOS:000350773000001					2021-04-07	
J	Saez, CA; Roncarati, F; Moenne, A; Moody, AJ; Brown, MT				Saez, Claudio A.; Roncarati, Francesca; Moenne, Alejandra; Moody, A. John; Brown, Murray T.			Copper-induced intra-specific oxidative damage and antioxidant responses in strains of the brown alga Ectocarpus siliculosus with different pollution histories	AQUATIC TOXICOLOGY			English	Article						Phaeophyceae; Ectocmpus siliculosus; Inter-population variation; Copper toxicity; Reactive oxygen metabolism	REACTIVE OXYGEN-METABOLISM; SUPEROXIDE-DISMUTASE; LESSONIA-NIGRESCENS; COMPLEXING LIGANDS; HEAVY-METALS; FAL ESTUARY; IN-VIVO; GLUTATHIONE; STRESS; ASCORBATE	Inter- and intra-specific variation in metal resistance has been observed in the ecologically and economically important marine brown macroalgae (Phaeophyceae), but the mechanisms of cellular tolerance are not well elucidated. To investigate inter-population responses of brown seaweeds to copper (Cu) pollution, the extent of oxidative damage and antioxidant responses were compared in three strains of the filamentous brown seaweed Ectocarpus siliculosus, the model organism for the algal class Phaeophyceae that diverged from other major eukaryotic groups over a billion year ago. Strains isolated from locations with different pollution histories (i.e. LIA, from a pristine site in Scotland; REP and Es524 from Cu-contaminated sites in England and Chile, respectively) were exposed to total dissolved Cu concentrations (Cur) of up to 2.4 mu M (equivalent to 128 nM Cu2+) for 10 d. LIA exhibited oxidative stress, with increases in hydrogen peroxide (H2O2) and lipid peroxidation (measured as TBARS levels), and decreased concentrations of photosynthetic pigments. Es524 presented no apparent oxidative damage whereas in REP, TBARS increased, revealing some level of oxidative damage. Adjustments to activities of enzymes and antioxidant compounds concentrations in Es524 and REP were strain and treatment dependent. Mitigation of oxidative stress in Es524 was by increased activities of superoxide dismutases (SOD) at low CUT, and catalase (CAT) and ascorbate peroxidase (APX) at all CUT, accompanied by higher levels of antioxidants (ascorbate, glutathione, phenolics) at higher Cur. In REP, only APX activity increased, as did the antioxidants. For the first time evidence is presented for distinctive oxidative stress defences under excess Cu in two populations of a species of brown seaweed from environments contaminated by Cu. (C) 2014 Elsevier B.V. All rights reserved.	[Saez, Claudio A.; Roncarati, Francesca; Brown, Murray T.] Univ Plymouth, Fac Sci & Environm, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England; [Saez, Claudio A.; Moenne, Alejandra] Univ Santiago Chile, Fac Quim & Biol, Dept Biol, Santiago, Chile; [Moody, A. John] Univ Plymouth, Fac Sci & Environm, Sch Biol Sci, Plymouth PL4 8AA, Devon, England	Brown, MT (corresponding author), Univ Plymouth, Fac Sci & Environm, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England.	mtbrown@plymouth.ac.uk	Saez, Claudio/F-5978-2015; Brown, Murray/K-5291-2014	Saez, Claudio/0000-0002-5037-3484; Moody, John/0000-0002-1994-7419; Brown, Murray/0000-0003-2655-8611	CONICYT Becas Chile Scholarship [72110557]; European Community 7th Framework ProgrammeEuropean Commission [235380]	We thank financial support for doctoral studies to C. A. Saez from CONICYT Becas Chile Scholarship (72110557) and from the European Community 7th Framework Programme (FP7/2007-2013), grant no. 235380, to F. Roncarati. Strains were kindly provided by Akira Peters's culture collection at the Station Biologique de Roscoff, France, and the Marine Biological Association of the UK (MBA).	Ali MB, 2006, PLANT CELL REP, V25, P1122, DOI 10.1007/s00299-006-0174-x; Andrade S, 2006, AQUAT TOXICOL, V78, P398, DOI 10.1016/j.aquatox.2006.04.006; Apel K, 2004, ANNU REV PLANT BIOL, V55, P373, DOI 10.1146/annurev.arplant.55.031903.141701; Babu TS, 2003, PLANT CELL PHYSIOL, V44, P1320, DOI 10.1093/pcp/pcg160; Benzie IFF, 1999, METHOD ENZYMOL, V299, P15; Brown MT, 2012, ECOTOXICOLOGY, V21, P591, DOI 10.1007/s10646-011-0819-6; Burkhead JL, 2009, NEW PHYTOL, V182, P799, DOI 10.1111/j.1469-8137.2009.02846.x; Burlinson FC, 2007, MAR POLLUT BULL, V54, P66, DOI 10.1016/j.marpolbul.2006.08.047; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Collen J, 1999, J PHYCOL, V35, P62, DOI 10.1046/j.1529-8817.1999.3510062.x; Collen J, 2001, J PHYCOL, V37, P474, DOI 10.1046/j.1529-8817.2001.037004474.x; Connan S, 2011, AQUAT TOXICOL, V104, P1, DOI 10.1016/j.aquatox.2011.03.016; Contreras L, 2005, J PHYCOL, V41, P1184, DOI 10.1111/j.1529-8817.2005.00151.x; Contreras L, 2009, AQUAT TOXICOL, V94, P94, DOI 10.1016/j.aquatox.2009.06.004; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; DePalma SGS, 2011, ECOTOX ENVIRON SAFE, V74, P230, DOI 10.1016/j.ecoenv.2010.12.003; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Foyer CH, 2011, PLANT PHYSIOL, V155, P2, DOI 10.1104/pp.110.167569; Gledhill M, 1997, J PHYCOL, V33, P2, DOI 10.1111/j.0022-3646.1997.00002.x; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; Gonzalez A, 2012, PLANT SIGNAL BEHAV, V7, P728, DOI 10.4161/psb.20355; Gonzalez A, 2012, PLANT PHYSIOL, V158, P1451, DOI 10.1104/pp.111.191759; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; Graham MH, 2007, OCEANOGR MAR BIOL, V45, P39; GRANT A, 1989, MAR POLLUT BULL, V20, P235, DOI 10.1016/0025-326X(89)90438-4; HEATH RL, 1968, ARCH BIOCHEM BIOPHYS, V125, P189, DOI 10.1016/0003-9861(68)90654-1; Karanov, 1997, P B ACAD SCI, V51, P121; Kupper H, 2002, J PHYCOL, V38, P429, DOI 10.1046/j.1529-8817.2002.01148.x; KUTHAN H, 1986, BIOCHEM J, V237, P175, DOI 10.1042/bj2370175; Leal MFC, 1999, LIMNOL OCEANOGR, V44, P1750, DOI 10.4319/lo.1999.44.7.1750; Lee MR, 2005, MAR ENVIRON RES, V59, P1, DOI 10.1016/j.marenvres.2004.01.002; MANN KH, 1973, SCIENCE, V182, P975, DOI 10.1126/science.182.4116.975; MCCORD JM, 1969, J BIOL CHEM, V244, P6049; Mellado M, 2012, PLANT PHYSIOL BIOCH, V51, P102, DOI 10.1016/j.plaphy.2011.10.007; Mikami K, 2013, INT J MOL SCI, V14, P13763, DOI 10.3390/ijms140713763; MOREL FMM, 1979, J PHYCOL, V15, P135, DOI 10.1111/j.0022-3646.1979.00135.x; Nagajyoti PC, 2010, ENVIRON CHEM LETT, V8, P199, DOI 10.1007/s10311-010-0297-8; Nielsen HD, 2010, MAR POLLUT BULL, V60, P710, DOI 10.1016/j.marpolbul.2009.11.025; Nielsen HD, 2003, PLANT CELL ENVIRON, V26, P1737, DOI 10.1046/j.1365-3040.2003.01091.x; Nielsen HD, 2003, NEW PHYTOL, V160, P157, DOI 10.1046/j.1469-8137.2003.00864.x; Noctor G, 1998, ANNU REV PLANT PHYS, V49, P249, DOI 10.1146/annurev.arplant.49.1.249; Noctor G, 2012, PLANT CELL ENVIRON, V35, P454, DOI 10.1111/j.1365-3040.2011.02400.x; Patsikka E, 2002, PLANT PHYSIOL, V129, P1359, DOI 10.1104/pp.004788; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; Pinto E, 2003, J PHYCOL, V39, P1008, DOI 10.1111/j.0022-3646.2003.02-193.x; Provasoli L, 1974, ALGAL PHYSL BIOCH, P741; Queval G, 2007, ANAL BIOCHEM, V363, P58, DOI 10.1016/j.ab.2007.01.005; Rahavi MR, 2011, FRONT PLANT SCI, V2, DOI 10.3389/fpls.2011.00091; Rainbow PS, 2011, AQUAT TOXICOL, V105, P455, DOI 10.1016/j.aquatox.2011.08.001; Rainbow PS, 2011, SCI TOTAL ENVIRON, V409, P1589, DOI 10.1016/j.scitotenv.2011.01.012; Ramirez M, 2005, MAR POLLUT BULL, V50, P62, DOI 10.1016/j.marpolbul.2004.08.010; Randhawa VK, 2001, CAN J MICROBIOL, V47, P987, DOI 10.1139/cjm-47-11-987; Ratkevicius N, 2003, PLANT CELL ENVIRON, V26, P1599, DOI 10.1046/j.1365-3040.2003.01073.x; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Sachindra NM, 2007, J AGR FOOD CHEM, V55, P8516, DOI 10.1021/jf071848a; SEELY GR, 1972, MAR BIOL, V12, P184, DOI 10.1007/BF00350754; Smirnoff N, 2005, BIOL SCI SER, P1, DOI 10.1002/9780470988565; SOMERFIELD PJ, 1994, MAR ECOL PROG SER, V105, P79, DOI 10.3354/meps105079; Sordet C, 2014, AQUAT TOXICOL, V150, P220, DOI 10.1016/j.aquatox.2014.02.018; TIPPING E, 1994, COMPUT GEOSCI, V20, P973, DOI 10.1016/0098-3004(94)90038-8; TITLEY JG, 1987, CONT SHELF RES, V7, P1363, DOI 10.1016/0278-4343(87)90041-0; VANALSTYNE KL, 1995, J CHEM ECOL, V21, P45, DOI 10.1007/BF02033661; Varma R, 2013, ESTUAR COAST SHELF S, V128, P33, DOI 10.1016/j.ecss.2013.05.014	65	37	38	2	62	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0166-445X	1879-1514		AQUAT TOXICOL	Aquat. Toxicol.	FEB	2015	159						81	89		10.1016/j.aquatox.2014.11.019			9	Marine & Freshwater Biology; Toxicology	Marine & Freshwater Biology; Toxicology	CB4IT	WOS:000349592100009	25521566	Green Published			2021-04-07	
J	Roncarati, F; Saez, CA; Greco, M; Gledhill, M; Bitonti, MB; Brown, MT				Roncarati, Francesca; Saez, Claudio A.; Greco, Maria; Gledhill, Martha; Bitonti, Maria B.; Brown, Murray T.			Response differences between Ectocarpus siliculosus populations to copper stress involve cellular exclusion and induction of the phytochelatin biosynthetic pathway	AQUATIC TOXICOLOGY			English	Article						Ectoconpus siliculosus; Brown algae; Copper; Phytochelatins; Glutathione; Inter-population variation	HEAVY-METAL DETOXIFICATION; MARINE FOULING ALGA; ULVA-COMPRESSA CHLOROPHYTA; GRACILARIOPSIS-LONGISSIMA; OXIDATIVE STRESS; FUCUS-SERRATUS; NORTHERN CHILE; MINE TAILINGS; BROWN-ALGAE; TOLERANCE	Some populations of brown seaweed species inhabit metal-polluted environments and can develop tolerance to metal stress, but the mechanisms by which this is accomplished are still to be elucidated. To address this, the responses of two strains of the model brown alga Ectocarpus siliculosus isolated from sites with different histories of metal contamination exposed to total copper (CUT) concentrations ranging between 0 and 2.4 mu M for 10 days were investigated. The synthesis of the metal-chelator phytochelatin (PCs) and relative levels of transcripts encoding the enzymes gamma-glutamylcysteine synthetase (gamma-GCS), glutathione synthase (GS) and phytochelatin synthase (PCS) that participate in the PC biosynthetic pathway were measured, along with the effects on growth, and adsorption and uptake of Cu. Growth of strain LIA, from a pristine site in Scotland, was inhibited to a greater extent, and at lower concentrations, than that of Es524, isolated from a Cu-contaminated site in Chile. Concentrations of intra-cellular Cu were higher and the exchangeable fraction was lower in LIA than Es524, especially at the highest exposure levels. Total glutathione concentrations increased in both strains with Cu exposure, whereas total PCs levels were higher in Es524 than LIA; PC2 and PC3 were detected in Es524 but PC2 only was found in LIA. The greater production and levels of polymerisation of PCs in Es524 can be explained by the up-regulation of genes encoding for key enzymes involved in the synthesis of PCs. In Es524 there was an increase in the transcripts of -gamma-GCS, GS and PCS, particularly under high Cu exposure, whereas in LIA4 transcripts of gamma-GCS1 increased only slightly, gamma-GCS2 and GS decreased and PCS did not change. The consequences of higher intra-cellular concentrations of Cu, lower production of PCs, and lower expression of enzymes involved in GSH-PCs synthesis may be contributing to an induced oxidative stress condition in LIA, which explains, at least in part, the observed sensitivity of LIA to Cu. Therefore, responses to Cu exposure in E. siliculosus relate to the contamination histories of the locations from where the strains were isolated and differences in Cu exclusion and PCs production are in part responsible for the development of intra-specific resistance. (C) 2014 Elsevier B.V. All rights reserved.	[Roncarati, Francesca; Saez, Claudio A.; Brown, Murray T.] Univ Plymouth, Fac Sci & Environm, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England; [Saez, Claudio A.] Univ Santiago Chile, Fac Quim & Biol, Dept Biol, Santiago, Chile; [Saez, Claudio A.] Univ Playa Ancha, Fac Ingn, Dept Medio Ambiente, Valparaiso, Chile; [Greco, Maria; Bitonti, Maria B.] Univ Calabria, Lab Plant Cytophysiol, I-87036 Cosenza, Italy; [Gledhill, Martha] GEOMAR, Helmholtz Ctr Ocean Res, D-24148 Cosenza, Italy	Brown, MT (corresponding author), Univ Plymouth, Fac Sci & Environm, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England.	mtbrown@plymouth.ac.uk	Bitonti, Maria/AAO-7345-2020; Saez, Claudio/F-5978-2015; Gledhill, Martha/D-2819-2009; Gledhill, Martha/AAG-6062-2021; Brown, Murray/K-5291-2014	Saez, Claudio/0000-0002-5037-3484; Gledhill, Martha/0000-0003-3859-2112; Gledhill, Martha/0000-0003-3859-2112; Brown, Murray/0000-0003-2655-8611	European CommunityEuropean Commission [235380]; CONICYT Becas Chile Scholarship [72110557]	We thank Dr. Alejandra Moenne for comments on an earlier draft. The research leading to these results has received funding from the European Community 7th Framework Programme (FP7/2007-2013) under grant agreement no. 235380. We thank financial support for doctoral studies to C. A. Saez from CONICYT Becas Chile Scholarship (72110557). Strains were kindly provided by the Marine Biological Association of the UK (MBA) and Akira Peters' culture collection at the Station Biologique de Roscoff, France.	Andrade S, 2006, AQUAT TOXICOL, V78, P398, DOI 10.1016/j.aquatox.2006.04.006; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Blum R, 2007, PLANT J, V49, P740, DOI 10.1111/j.1365-313X.2006.02993.x; Brown MT, 2003, AQUAT TOXICOL, V64, P201, DOI 10.1016/S0166-445X(03)00054-7; Brown MT, 2012, ECOTOXICOLOGY, V21, P591, DOI 10.1007/s10646-011-0819-6; Brunetti P, 2011, J EXP BOT, V62, P5509, DOI 10.1093/jxb/err228; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Clemens S, 2006, BIOCHIMIE, V88, P1707, DOI 10.1016/j.biochi.2006.07.003; Cobbett C, 2002, ANNU REV PLANT BIOL, V53, P159, DOI 10.1146/annurev.arplant.53.100301.135154; Cobbett CS, 2000, PLANT PHYSIOL, V123, P825, DOI 10.1104/pp.123.3.825; Cobbett CS, 2001, IUBMB LIFE, V51, P183, DOI 10.1080/152165401753544250; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho S.M., 2012, COLD SPRING HARBOR P, V1, P258; Correa JA, 1999, J APPL PHYCOL, V11, P57, DOI 10.1023/A:1008027610826; Davis TA, 2003, WATER RES, V37, P4311, DOI 10.1016/S0043-1354(03)00293-8; Dechamps C, 2008, PLANT SOIL, V310, P211, DOI 10.1007/s11104-008-9648-7; DEKNECHT JA, 1994, PLANT PHYSIOL, V104, P255, DOI 10.1104/pp.104.1.255; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; DRING MJ, 1967, J MAR BIOL ASSOC UK, V47, P501, DOI 10.1017/S002531540003513X; Garcia-Rios V, 2007, AQUAT TOXICOL, V81, P65, DOI 10.1016/j.aquatox.2006.11.001; GEKELER W, 1988, ARCH MICROBIOL, V150, P197, DOI 10.1007/BF00425162; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; Gledhill M, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00155; Gonzalez A, 2010, PLANT CELL ENVIRON, V33, P1627, DOI 10.1111/j.1365-3040.2010.02169.x; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; HALL A, 1980, NEW PHYTOL, V85, P73, DOI 10.1111/j.1469-8137.1980.tb04449.x; Hassler CS, 2004, LIMNOL OCEANOGR-METH, V2, P237, DOI 10.4319/lom.2004.2.237; HOLMES MA, 1991, MAR ENVIRON RES, V31, P55, DOI 10.1016/0141-1136(91)90005-S; Kobayashi I, 2006, MAR BIOTECHNOL, V8, P94, DOI 10.1007/s10126-005-5092-3; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lee MR, 2005, MAR ENVIRON RES, V59, P1, DOI 10.1016/j.marenvres.2004.01.002; Lee SM, 2005, J PLANT BIOL, V48, P32, DOI 10.1007/BF03030562; Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262; Malea P, 2006, MAR ENVIRON RES, V62, P45, DOI 10.1016/j.marenvres.2006.03.002; Mellado M, 2012, PLANT PHYSIOL BIOCH, V51, P102, DOI 10.1016/j.plaphy.2011.10.007; MOREL FMM, 1979, J PHYCOL, V15, P135, DOI 10.1111/j.0022-3646.1979.00135.x; Nielsen HD, 2003, PLANT CELL ENVIRON, V26, P1737, DOI 10.1046/j.1365-3040.2003.01091.x; Nielsen HD, 2003, NEW PHYTOL, V160, P157, DOI 10.1046/j.1469-8137.2003.00864.x; Noctor G, 2012, PLANT CELL ENVIRON, V35, P454, DOI 10.1111/j.1365-3040.2011.02400.x; Pawlik-Skowronska B, 2004, AQUAT TOXICOL, V70, P201, DOI 10.1016/j.aquatox.2004.09.003; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Pinto E, 2003, J PHYCOL, V39, P1008, DOI 10.1111/j.0022-3646.2003.02-193.x; PROVASOLI L, 1974, ALGAL PHYSL BIOCH, P741; Ramirez M, 2005, MAR POLLUT BULL, V50, P62, DOI 10.1016/j.marpolbul.2004.08.010; Rea PA, 2012, PHYSIOL PLANTARUM, V145, P154, DOI 10.1111/j.1399-3054.2012.01571.x; REED RH, 1983, J EXP MAR BIOL ECOL, V69, P85, DOI 10.1016/0022-0981(83)90173-9; Rigouin C, 2013, MOL BIOCHEM PARASIT, V191, P1, DOI 10.1016/j.molbiopara.2013.07.003; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; RUSSELL G, 1983, MAR ECOL PROG SER, V11, P181, DOI 10.3354/meps011181; RUSSELL G, 1967, HELGOLAND WISS MEER, V15, P155, DOI 10.1007/BF01618619; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Scarano G, 2002, BIOMETALS, V15, P145, DOI 10.1023/A:1015288000218; STEFFENS JC, 1986, J BIOL CHEM, V261, P13879; Tennstedt P, 2009, PLANT PHYSIOL, V149, P938, DOI 10.1104/pp.108.127472; Torres MA, 2008, ECOTOX ENVIRON SAFE, V71, P1, DOI 10.1016/j.ecoenv.2008.05.009; Vasconcelos MTSD, 2001, MAR CHEM, V74, P65, DOI 10.1016/S0304-4203(00)00096-7; Yong YS, 2013, J APPL PHYCOL, V25, P1831, DOI 10.1007/s10811-013-0022-7; Zhao CZ, 2010, PLANT CELL ENVIRON, V33, P877, DOI 10.1111/j.1365-3040.2009.02113.x	60	22	22	4	45	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	0166-445X	1879-1514		AQUAT TOXICOL	Aquat. Toxicol.	FEB	2015	159						167	175		10.1016/j.aquatox.2014.12.009			9	Marine & Freshwater Biology; Toxicology	Marine & Freshwater Biology; Toxicology	CB4IT	WOS:000349592100018	25546007	Green Published			2021-04-07	
J	Saez, CA; Ramesh, K; Greco, M; Bitonti, MB; Brown, MT				Saez, Claudio A.; Ramesh, Kirti; Greco, Maria; Bitonti, M. Beatrice; Brown, Murray T.			Enzymatic antioxidant defences are transcriptionally regulated in Es524, a copper-tolerant strain of Ectocarpus siliculosus (Ectocarpales, Phaeophyceae)	PHYCOLOGIA			English	Article						Antioxidant metabolism; Gene expression; Interpopulation; Metal tolerance; Phaeophyceae	DIFFERENT POLLUTION HISTORIES; SUPEROXIDE-DISMUTASE; NORTHERN CHILE; FUCUS-SERRATUS; MINE TAILINGS; RESPONSES; STRESS; METAL; ULTRASTRUCTURE; GROWTH	Previous investigations have revealed that Ectocarpus siliculosus strain Es524, isolated from a highly copper (Cu)-polluted location in Chile, displays higher activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) under Cu excess. This partly explains its enhanced tolerance compared with other E. siliculosus strains. However, the transcriptomic basis behind the activity of these antioxidant enzymes is still unknown. We have conducted laboratory experiments with Es524 under control conditions and exposure to 2.4 mu M total Cu. Under Cu exposure, Es524 showed a decrease in cell length and cell plasmolysis, revealing a certain level of cytophysiological stress. The genes encoding for iron-SOD, APX and CAT were overexpressed under Cu excess, demonstrating that higher activities of these enzymes are transcriptionally regulated. It is now more apparent that the induction of SOD, APX and CAT are conserved molecular and biochemical mechanisms developed after a long history of Cu exposure which is responsible, at least in part, for intraspecific Cu tolerance in E. siliculosus.	[Saez, Claudio A.] Univ Playa Ancha, Lab Ambiental Estudios Costeros, Dept Medio Ambiente, Fac Ingn, Valparaiso, Chile; [Saez, Claudio A.] Univ Playa Ancha, Ctr Estudios Avanzados, Vina Del Mar, Chile; [Saez, Claudio A.; Ramesh, Kirti; Brown, Murray T.] Univ Plymouth, Sch Marine Sci & Engn, Fac Sci & Environm, Plymouth PL4 8AA, Devon, England; [Greco, Maria; Bitonti, M. Beatrice] Univ Calabria, Lab Plant Cytophysiol, Dept Biol Ecol & Earth Sci, I-87036 Cosenza, Italy	Saez, CA (corresponding author), Univ Playa Ancha, Lab Ambiental Estudios Costeros, Dept Medio Ambiente, Fac Ingn, Casilla 34-V, Valparaiso, Chile.	claudio.saez@upla.cl	Saez, Claudio/F-5978-2015; Bitonti, Maria/AAO-7345-2020; Brown, Murray/K-5291-2014	Saez, Claudio/0000-0002-5037-3484; Brown, Murray/0000-0003-2655-8611	CONICYT Becas Chile Scholarship [72110557]	C.A.S. gratefully acknowledges the financial support from CONICYT Becas Chile Scholarship (72110557).	Alaoui-Sosse B, 2004, PLANT SCI, V166, P1213, DOI 10.1016/j.plantsci.2003.12.032; Bakthavatchalu V, 2012, ONCOGENE, V31, P2129, DOI 10.1038/onc.2011.407; Burkhead JL, 2009, NEW PHYTOL, V182, P799, DOI 10.1111/j.1469-8137.2009.02846.x; Chiappetta A., 2006, BIOL MARINA MEDITERR, V39, P39; CHUNG IK, 1986, MAR POLLUT BULL, V17, P213, DOI 10.1016/0025-326X(86)90603-X; Correa JA, 1999, J APPL PHYCOL, V11, P57, DOI 10.1023/A:1008027610826; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; Greco M, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0101190; HEUMANN HG, 1987, PROTOPLASMA, V136, P37, DOI 10.1007/BF01276316; IBM, 2012, SPSS 21; Kuo WY, 2013, NEW PHYTOL, V197, P99, DOI 10.1111/j.1469-8137.2012.04369.x; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lee MR, 2005, MAR ENVIRON RES, V59, P1, DOI 10.1016/j.marenvres.2004.01.002; Mason MG, 2006, P NATL ACAD SCI USA, V103, P708, DOI 10.1073/pnas.0506562103; Mellado M, 2012, PLANT PHYSIOL BIOCH, V51, P102, DOI 10.1016/j.plaphy.2011.10.007; MOREL FMM, 1979, J PHYCOL, V15, P135, DOI 10.1111/j.0022-3646.1979.00135.x; National Institutes of Health, 2011, IM J VERS 1 45K; Nielsen HD, 2003, PLANT CELL ENVIRON, V26, P1737, DOI 10.1046/j.1365-3040.2003.01091.x; Nielsen HD, 2003, NEW PHYTOL, V160, P157, DOI 10.1046/j.1469-8137.2003.00864.x; Ortega KJ, 2014, REV BIOL MAR OCEANOG, V49, P129, DOI 10.4067/S0718-19572014000100014; OUZOUNIDOU G, 1995, ENVIRON EXP BOT, V35, P167, DOI 10.1016/0098-8472(94)00049-B; PERCIVAL E., 1981, ENCY PLANT PHYSL B, V13B, P277; Pinto E, 2003, J PHYCOL, V39, P1008, DOI 10.1111/j.0022-3646.2003.02-193.x; RIISGARD HU, 1979, MAR BIOL, V50, P189, DOI 10.1007/BF00397826; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Roncarati F, 2015, AQUAT TOXICOL, V159, P167, DOI 10.1016/j.aquatox.2014.12.009; Saez CA, 2015, ENVIRON POLLUT, V199, P130, DOI 10.1016/j.envpol.2015.01.026; Saez CA, 2015, AQUAT TOXICOL, V159, P81, DOI 10.1016/j.aquatox.2014.11.019; Saez CA, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0050170; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; Smith SDA, 1996, AUST J ECOL, V21, P144, DOI 10.1111/j.1442-9993.1996.tb00595.x; Sorbo S, 2011, PLANT BIOSYST, V145, P461, DOI 10.1080/11263504.2011.558722; Tarakhovskaya ER, 2015, PHYCOLOGIA, V54, P417, DOI 10.2216/15-35.1; Tarhanen S, 1998, ANN BOT-LONDON, V82, P735, DOI 10.1006/anbo.1998.0734; VISVIKI I, 1994, ARCH ENVIRON CON TOX, V26, P154; WAINWRIGHT SJ, 1977, J EXP BOT, V28, P1029, DOI 10.1093/jxb/28.4.1029; Zhang XL, 2011, SCI HORTIC-AMSTERDAM, V129, P656, DOI 10.1016/j.scienta.2011.05.009	37	12	12	0	3	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia		2015	54	4					425	429		10.2216/15-30.1			5	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	CR8QD	WOS:000361617900011					2021-04-07	
J	Jernigan, A; Hestekin, C				Jernigan, Alice; Hestekin, Christa			Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species	JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY			English	Article							RAPID IDENTIFICATION; BACTERIA; MUTATIONS; CHEESE; DNA	Capillary electrophoresis single-strand conformational polymorphism (CE-SSCP) was explored as a fast and inexpensive method to differentiate both prokaryotic (blue-green) and eukaryotic (green and brown) algae. A selection of two blue-green algae (Nostoc muscorum and Anabaena inaequalis), five green algae (Chlorella vulgaris, Oedogonium foveolatum, Mougeotia sp., Scenedesmus quadricauda, and Ulothrix fimbriata), and one brown algae (Ectocarpus sp.) were examined and CE-SSCP electropherogram "fingerprints" were compared to each other for two variable regions of either the 16S or 18S rDNA gene. The electropherogram patterns were remarkably stable and consistent for each particular species. The patterns were unique to each species, although some common features were observed between the different types of algae. CE-SSCP could be a useful method for monitoring changes in an algae species over time as potential shifts in species occurred.	[Jernigan, Alice; Hestekin, Christa] Univ Arkansas, Dept Chem Engn, Fayetteville, AR 72701 USA	Hestekin, C (corresponding author), Univ Arkansas, Dept Chem Engn, Fayetteville, AR 72701 USA.	chesteki@uark.edu					Abbott I.A., 1992, MARINE ALGAE CALIFOR; Albarghouthi M, 2001, ELECTROPHORESIS, V22, P737, DOI 10.1002/1522-2683(200102)22:4<737::AID-ELPS737>3.0.CO;2-S; BAKKER FT, 1994, MOL PHYLOGENET EVOL, V3, P365, DOI 10.1006/mpev.1994.1043; Becker EW, 1993, MICROALGAE BIOTECHNO; Chakravorty S, 2007, J MICROBIOL METH, V69, P330, DOI 10.1016/j.mimet.2007.02.005; Christner BC, 2003, EXTREMOPHILES, V7, P177, DOI 10.1007/s00792-002-0309-0; Delbes C, 2007, APPL ENVIRON MICROB, V73, P1882, DOI 10.1128/AEM.01716-06; Doi K, 2004, ELECTROPHORESIS, V25, P833, DOI 10.1002/elps.200305721; Duthoit F, 2005, J APPL MICROBIOL, V98, P1198, DOI 10.1111/j.1365-2672.2005.02575.x; Ghozzi R, 1999, J CLIN MICROBIOL, V37, P3374, DOI 10.1128/JCM.37.10.3374-3379.1999; Herrera-Sepulveda A, 2013, ENVIRON SCI POLLUT R, V20, P6863, DOI 10.1007/s11356-012-1033-7; Hillebrand H, 1999, J PHYCOL, V35, P403, DOI 10.1046/j.1529-8817.1999.3520403.x; King S, 2005, J MICROBIOL METH, V60, P83, DOI 10.1016/j.mimet.2004.08.014; Krothapalli S, 2012, J MICROBIOL METH, V91, P147, DOI 10.1016/j.mimet.2012.07.021; Lee R. E., 2008, PHYCOLOGY; LUND J. W. G., 1958, HYDROBIOLOGIA, V11, P143, DOI 10.1007/BF00007865; Martin W, 2002, P NATL ACAD SCI USA, V99, P12246, DOI 10.1073/pnas.182432999; ORITA M, 1989, P NATL ACAD SCI USA, V86, P2766, DOI 10.1073/pnas.86.8.2766; ROWAN R, 1991, MAR ECOL PROG SER, V71, P65, DOI 10.3354/meps071065; Schwieger F, 1998, APPL ENVIRON MICROB, V64, P4870; Tian HJ, 2000, GENOMICS, V63, P25, DOI 10.1006/geno.1999.6067; Turenne CY, 2000, J CLIN MICROBIOL, V38, P513, DOI 10.1128/JCM.38.2.513-520.2000; WIDJOJOATMODJO MN, 1994, J CLIN MICROBIOL, V32, P3002, DOI 10.1128/JCM.32.12.3002-3007.1994; WOESE CR, 1987, MICROBIOL REV, V51, P221, DOI 10.1128/MMBR.51.2.221-271.1987	24	4	4	0	11	HINDAWI LTD	LONDON	ADAM HOUSE, 3RD FLR, 1 FITZROY SQ, LONDON, W1T 5HF, ENGLAND	2090-8865	2090-8873		J ANAL METHODS CHEM	J. Anal. Methods Chem.		2015	2015								272964	10.1155/2015/272964			7	Chemistry, Analytical	Chemistry	CJ9UC	WOS:000355847700001	26101693	DOAJ Gold, Green Published			2021-04-07	
J	Debowski, M; Zielinski, M; Rokicka, M; Kupczyk, K				Debowski, Marcin; Zielinski, Marcin; Rokicka, Magdalena; Kupczyk, Karolina			The Possibility of Using Macroalgae Biomass from Natural Reservoirs as a Substrate in the Methane Fermentation Process	INTERNATIONAL JOURNAL OF GREEN ENERGY			English	Article						Macroalgae biomass; Taxonomic structure; Methane fermentation; Biogas	ANAEROBIC CO-DIGESTION; BIOGAS; ALGAE; MICROALGAE; BIOFUELS; YIELD	The objective of this study was to determine the possibility of applying macroalgae biomass originating from Bay of Puck, Poland, as a substrate in the methane fermentation process and to verify the impact of taxonomic structure and other physicochemical traits of the analyzed biomass on the technological effectiveness of the fermentation process. The efficiency of methane fermentation was determined using respirometric kits. In all periods of the vegetative season, the structure of macrophytobenthos was predominated by brown algae of the family Ectocarpaceae, namely Pilayella litoralis and Ectocarpus sp. The production of biogas fitted within a narrow range of 266.53 +/- 16.43 cm(3)/g o.d.m. during fermentation of algae harvested in September and 285.71 +/- 11.35 cm(3)/g o.d.m. in that from August. The percentage content of methane in biogas ranged from 57.28 +/- 1.75% to 59.22 +/- 2.84%. Regardless of macroalgae biomass harvest period, the postfermented sludge was characterized by similar values of the analyzed parameters.	[Debowski, Marcin; Zielinski, Marcin; Rokicka, Magdalena; Kupczyk, Karolina] Univ Warmia & Mazury, Dept Environm Engn, PL-10720 Olsztyn, Poland	Rokicka, M (corresponding author), Univ Warmia & Mazury, Dept Environm Engn, Warszawska 117 St, PL-10720 Olsztyn, Poland.	magdalena.rokicka@uwm.edu.pl		Debowski, Marcin/0000-0002-9719-6390; Zielinski, Marcin/0000-0003-1132-1013	OP Innovative Economy;  [POIG.01.01.02-00-016/08]	This research was carried out under the Key Project No. POIG.01.01.02-00-016/08 titled "Model agroenergy complexes as an example of distributed cogeneration based on local and renewable energy sources." The project was financed under the OP Innovative Economy.	Borjesson P, 2006, BIOMASS BIOENERG, V30, P469, DOI 10.1016/j.biombioe.2005.11.014; Brennan L, 2010, RENEW SUST ENERG REV, V14, P557, DOI 10.1016/j.rser.2009.10.009; Bruhn A, 2011, BIORESOURCE TECHNOL, V102, P2595, DOI 10.1016/j.biortech.2010.10.010; Campbell JE, 2009, SCIENCE, V324, P1055, DOI 10.1126/science.1168885; Carver SM, 2011, BIODEGRADATION, V22, P805, DOI 10.1007/s10532-010-9419-z; CHYNOWETH DP, 1993, BIOMASS BIOENERG, V5, P95, DOI 10.1016/0961-9534(93)90010-2; Debowski M, 2013, ALGAE BIOMASS APPL S; Debowski M, 2013, RENEW SUST ENERG REV, V27, P596, DOI 10.1016/j.rser.2013.07.029; Debowski M, 2012, ARCH ENVIRON PROT, V38, P99, DOI 10.2478/v10265-012-0033-5; Gonzalez-Fernandez C, 2011, APPL ENERG, V88, P3448, DOI 10.1016/j.apenergy.2010.12.035; Grala A, 2012, POL J ENVIRON STUD, V21, P363; Guo L, 2007, SCIENCE, V317, P1166, DOI 10.1126/science.317.5842.1166; Lee J. W., 2013, HIGH VALUE ALGAL PRO, DOI [10.1007/978-1-4614-3348-4_2, DOI 10.1007/978-1-4614-3348-4_2]; Li Y, 2008, BIOTECHNOL PROGR, V24, P815, DOI 10.1021/bp070371k; Mata-Alvarez J, 2000, BIORESOURCE TECHNOL, V74, P3, DOI 10.1016/S0960-8524(00)00023-7; Parmar A, 2011, BIORESOURCE TECHNOL, V102, P10163, DOI 10.1016/j.biortech.2011.08.030; Pienkos PT, 2009, BIOFUEL BIOPROD BIOR, V3, P431, DOI 10.1002/bbb.159; Ras M, 2011, BIORESOURCE TECHNOL, V102, P200, DOI 10.1016/j.biortech.2010.06.146; Rittmann BE, 2008, BIOTECHNOL BIOENG, V100, P203, DOI 10.1002/bit.21875; Sialve B, 2009, BIOTECHNOL ADV, V27, P409, DOI 10.1016/j.biotechadv.2009.03.001; Singh J, 2010, RENEW SUST ENERG REV, V14, P2596, DOI 10.1016/j.rser.2010.06.014; Singh NK, 2011, AGRON SUSTAIN DEV, V31, P605, DOI 10.1007/s13593-011-0018-0; Smith VH, 2010, TRENDS ECOL EVOL, V25, P301, DOI 10.1016/j.tree.2009.11.007; Stephens E., 2008, NAT BIOTECHNOL, V28, P126; Vergara-Fernandez A, 2008, BIOMASS BIOENERG, V32, P338, DOI 10.1016/j.biombioe.2007.10.005; Wiley PE, 2011, WATER ENVIRON RES, V83, P326, DOI 10.2175/106143010X12780288628615; WISE DL, 1979, RESOUR RECOV CONSERV, V4, P217, DOI 10.1016/0304-3967(79)90002-7; Wu X, 2010, BIORESOURCE TECHNOL, V101, P4042, DOI 10.1016/j.biortech.2010.01.052; Yen HW, 2007, BIORESOURCE TECHNOL, V98, P130, DOI 10.1016/j.biortech.2005.11.010; Yuan XZ, 2011, ENERG ENVIRON SCI, V4, P1511, DOI 10.1039/c0ee00452a; Zeng SJ, 2010, J HAZARD MATER, V178, P89, DOI 10.1016/j.jhazmat.2010.01.047	31	6	6	0	9	TAYLOR & FRANCIS INC	PHILADELPHIA	530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA	1543-5075	1543-5083		INT J GREEN ENERGY	Int. J. Green Energy		2015	12	9					970	977		10.1080/15435075.2014.891122			8	Thermodynamics; Green & Sustainable Science & Technology; Energy & Fuels	Thermodynamics; Science & Technology - Other Topics; Energy & Fuels	CG8PM	WOS:000353571100010					2021-04-07	
J	Prigent, S; Collet, G; Dittami, SM; Delage, L; de Corny, FE; Dameron, O; Eveillard, D; Thiele, S; Cambefort, J; Boyen, C; Siegel, A; Tonon, T				Prigent, Sylvain; Collet, Guillaume; Dittami, Simon M.; Delage, Ludovic; de Corny, Floriane Ethis; Dameron, Olivier; Eveillard, Damien; Thiele, Sven; Cambefort, Jeanne; Boyen, Catherine; Siegel, Anne; Tonon, Thierry			The genome-scale metabolic network of Ectocarpus siliculosus (EctoGEM): a resource to study brown algal physiology and beyond	PLANT JOURNAL			English	Article						brown algae; Ectocarpus siliculosus; metabolic network; gap filling; synthesis of aromatic amino acids; molybdenum co-factor biosynthesis; systems biology	PHENYLALANINE BIOSYNTHESIS; MANNITOL METABOLISM; RECONSTRUCTION; ARABIDOPSIS; IDENTIFICATION; EVOLUTION; INSIGHTS; REVEALS; DEHYDROGENASE; PHAEOPHYCEAE	Brown algae (stramenopiles) are key players in intertidal ecosystems, and represent a source of biomass with several industrial applications. Ectocarpus siliculosus is a model to study the biology of these organisms. Its genome has been sequenced and a number of post-genomic tools have been implemented. Based on this knowledge, we report the reconstruction and analysis of a genome-scale metabolic network for E. siliculosus, EctoGEM (http://ectogem.irisa.fr). This atlas of metabolic pathways consists of 1866 reactions and 2020 metabolites, and its construction was performed by means of an integrative computational approach for identifying metabolic pathways, gap filling and manual refinement. The capability of the network to produce biomass was validated by flux balance analysis. EctoGEM enabled the reannotation of 56 genes within the E. siliculosus genome, and shed light on the evolution of metabolic processes. For example, E. siliculosus has the potential to produce phenylalanine and tyrosine from prephenate and arogenate, but does not possess a phenylalanine hydroxylase, as is found in other stramenopiles. It also possesses the complete eukaryote molybdenum co-factor biosynthesis pathway, as well as a second molybdopterin synthase that was most likely acquired via horizontal gene transfer from cyanobacteria by a common ancestor of stramenopiles. EctoGEM represents an evolving community resource to gain deeper understanding of the biology of brown algae and the diversification of physiological processes. The integrative computational method applied for its reconstruction will be valuable to set up similar approaches for other organisms distant from biological benchmark models.	[Prigent, Sylvain; Collet, Guillaume; de Corny, Floriane Ethis; Dameron, Olivier; Cambefort, Jeanne; Siegel, Anne] Univ Rennes 1, IRISA UMR 6074, F-35042 Rennes, France; [Prigent, Sylvain; Collet, Guillaume; de Corny, Floriane Ethis; Dameron, Olivier; Cambefort, Jeanne; Siegel, Anne] CNRS, IRISA UMR 6074, F-35042 Rennes, France; [Prigent, Sylvain; Collet, Guillaume; de Corny, Floriane Ethis; Dameron, Olivier; Thiele, Sven; Cambefort, Jeanne; Siegel, Anne] Ctr Rennes Bretagne Atlantique, Projet Dyliss, INRIA, F-35042 Rennes, France; [Dittami, Simon M.; Delage, Ludovic; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, Sorbonne Univ, UMR 8227, Stn Biol Roscoff, F-29688 Roscoff, France; [Dittami, Simon M.; Delage, Ludovic; Boyen, Catherine; Tonon, Thierry] CNRS, Stn Biol Roscoff, UMR 8227, F-29688 Roscoff, France; [Eveillard, Damien] Univ Nantes, LINA UMR 6241, F-44322 Nantes, France	Siegel, A (corresponding author), Univ Rennes 1, IRISA UMR 6074, Campus Beaulieu, F-35042 Rennes, France.	anne.siegel@irisa.fr; tonon@sb-roscoff.fr	eveillard, damien/AAQ-2363-2020; Tonon, Thierry/A-3214-2009	eveillard, damien/0000-0002-8162-7360; Tonon, Thierry/0000-0002-1454-6018; Prigent, Sylvain/0000-0001-5146-0347; Thiele, Sven/0000-0002-5812-6963; Dameron, Olivier/0000-0001-8959-7189	French National Research AgencyFrench National Research Agency (ANR) [ANR-10-BTBR-02-11]; University of Rennes 1; Inria; BIOTEMPO project [ANR-10-BLANC-0218]	This work was supported by the French National Research Agency via the investment expenditure programme IDEALG (ANR-10-BTBR-02-11). S. P. received a PhD grant from the University of Rennes 1, and S. T. was supported by postdoctoral funding from Inria. The BIOTEMPO project (ANR-10-BLANC-0218) supported the methodological studies.	Agren R, 2013, PLOS COMPUT BIOL, V9, DOI 10.1371/journal.pcbi.1002980; Berglund AC, 2008, NUCLEIC ACIDS RES, V36, pD263, DOI 10.1093/nar/gkm1020; Billoud B, 2014, NUCLEIC ACIDS RES, V42, P417, DOI 10.1093/nar/gkt856; Caspi R, 2013, FEMS MICROBIOL LETT, V345, P85, DOI 10.1111/1574-6968.12194; Caspi R, 2012, NUCLEIC ACIDS RES, V40, pD742, DOI 10.1093/nar/gkr1014; Chang RL, 2011, MOL SYST BIOL, V7, DOI 10.1038/msb.2011.52; Cho MH, 2007, J BIOL CHEM, V282, P30827, DOI 10.1074/jbc.M702662200; Christian N, 2009, MOL BIOSYST, V5, P1889, DOI [10.1039/b915913b, 10.1039/B915913b]; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Collakova E, 2012, PLANT SCI, V191, P53, DOI 10.1016/j.plantsci.2012.04.010; Dal'Molin CGD, 2013, CURR OPIN BIOTECH, V24, P271, DOI 10.1016/j.copbio.2012.08.007; Dal'Molin CGD, 2011, BMC GENOMICS, V12, DOI 10.1186/1471-2164-12-S4-S5; Dal'Molin CGD, 2010, PLANT PHYSIOL, V152, P579, DOI 10.1104/pp.109.148817; Dereeper A, 2008, NUCLEIC ACIDS RES, V36, pW465, DOI 10.1093/nar/gkn180; Dittami SM, 2014, MOL ECOL, V23, P1656, DOI 10.1111/mec.12670; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Ebrahim A, 2013, BMC SYST BIOL, V7, DOI 10.1186/1752-0509-7-74; Edwards JS, 2000, P NATL ACAD SCI USA, V97, P5528, DOI 10.1073/pnas.97.10.5528; Enquist-Newman M, 2014, NATURE, V505, P239, DOI 10.1038/nature12771; Fabris M, 2012, PLANT J, V70, P1004, DOI 10.1111/j.1365-313X.2012.04941.x; Fischer S., 2011, CURR PROTOC BIOINFOR, V6; Forster J, 2003, GENOME RES, V13, P244, DOI 10.1101/gr.234503; Graham MH, 2007, P NATL ACAD SCI USA, V104, P16576, DOI 10.1073/pnas.0704778104; Graindorge M, 2010, FEBS LETT, V584, P4357, DOI 10.1016/j.febslet.2010.09.037; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Groisillier A, 2014, J EXP BOT, V65, P559, DOI 10.1093/jxb/ert405; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Henry CS, 2010, NAT BIOTECHNOL, V28, P977, DOI 10.1038/nbt.1672; Iwamoto K, 2005, MAR BIOTECHNOL, V7, P407, DOI 10.1007/s10126-005-0029-4; Karp Peter D, 2002, Bioinformatics, V18 Suppl 1, pS225; Krumholz EW, 2012, J EXP BOT, V63, P2353, DOI 10.1093/jxb/err407; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Li L, 2003, GENOME RES, V13, P2178, DOI 10.1101/gr.1224503; Loira N, 2012, BMC SYST BIOL, V6, DOI 10.1186/1752-0509-6-35; Maeda A, 2012, NAT CHEM BIOL, V8, P170, DOI 10.1038/nchembio.759; Meslet-Cladiere L, 2013, PLANT CELL, V25, P3089, DOI 10.1105/tpc.113.111336; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Orth JD, 2010, NAT BIOTECHNOL, V28, P245, DOI 10.1038/nbt.1614; Ostlund G, 2010, NUCLEIC ACIDS RES, V38, pD196, DOI 10.1093/nar/gkp931; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Poolman MG, 2009, PLANT PHYSIOL, V151, P1570, DOI 10.1104/pp.109.141267; Qian WQ, 2007, PLANT J, V49, P399, DOI 10.1111/j.1365-313X.2006.02967.x; Rippert P, 2002, EUR J BIOCHEM, V269, P4753, DOI 10.1046/j.1432-1033.2002.03172.x; Rippert P, 2009, PLANT PHYSIOL, V149, P1251, DOI 10.1104/pp.108.130070; Ritter A, 2014, BMC PLANT BIOL, V14, DOI 10.1186/1471-2229-14-116; Rousvoal S, 2011, PLANTA, V233, P261, DOI 10.1007/s00425-010-1295-6; Schaub T., 2009, LNCS, V5649; Schwarz G, 2006, ANNU REV PLANT BIOL, V57, P623, DOI 10.1146/annurev.arplant.57.032905.105437; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Tejada-Jimenez M, 2011, P NATL ACAD SCI USA, V108, P6420, DOI 10.1073/pnas.1100700108; Tenhaken R, 2011, J BIOL CHEM, V286, P16707, DOI 10.1074/jbc.M111.230979; Thiele I, 2010, NAT PROTOC, V5, P93, DOI 10.1038/nprot.2009.203; Tonon T, 2011, OMICS, V15, P883, DOI 10.1089/omi.2011.0089; Vallenet D, 2009, DATABASE-OXFORD, DOI 10.1093/database/bap021; van Hal JW, 2014, TRENDS BIOTECHNOL, V32, P231, DOI 10.1016/j.tibtech.2014.02.007; Wargacki AJ, 2012, SCIENCE, V335, P308, DOI 10.1126/science.1214547; Wei N, 2013, TRENDS BIOTECHNOL, V31, P70, DOI 10.1016/j.tibtech.2012.10.009; Yoo H, 2013, NAT COMMUN, V4, DOI 10.1038/ncomms3833	66	22	22	1	57	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0960-7412	1365-313X		PLANT J	Plant J.	OCT	2014	80	2					367	381		10.1111/tpj.12627			15	Plant Sciences	Plant Sciences	AQ5LF	WOS:000342849800015	25065645	Bronze			2021-04-07	
J	Deniaud-Bouet, E; Kervarec, N; Michel, G; Tonon, T; Kloareg, B; Herve, C				Deniaud-Bouet, Estelle; Kervarec, Nelly; Michel, Gurvan; Tonon, Thierry; Kloareg, Bernard; Herve, Cecile			Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae	ANNALS OF BOTANY			English	Article						Brown algae; Fucales; cell wall architecture; sequential extractions; sulfated fucan; fucose-containing sulfated polysacharides; FCSP; extracellular matrix; ECM; Himanthalia elongata; plant cell wall evolution	LAMINARIA-DIGITATA PHAEOPHYCEAE; SEAWEED ECKLONIA-KUROME; ANTICOAGULANT ACTIVITY; MOLECULAR-WEIGHT; ECTOCARPUS-SILICULOSUS; EXTRACTION METHODS; FUCUS-VESICULOSUS; SULFATED FUCANS; FUCOIDAN; EVOLUTION	Background and Aims Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall. They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates). How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear. Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae. Methods Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed. Dedicated enzymatic degradations were used to release and identify cell wall partners. This approach was complemented by systematic chromatographic analysis to study polymer inter-links further. An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made. Key Results The data indicate that FCSPs are tightly associated with proteins and cellulose within the walls. Alginates are associated with most phenolic compounds. The sulfated fucans from H. elongata were shown to have a regular alpha-(1 --> 3) backbone structure, while an alternating alpha-(1 --> 3), (1 --> 4) structure has been described in some brown algae from the order Fucales. Conclusions The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components. Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress. The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.	[Deniaud-Bouet, Estelle; Michel, Gurvan; Tonon, Thierry; Kloareg, Bernard; Herve, Cecile] Univ Paris 06, Sorbonne Univ, UMR Integrat Biol Marine Models 8227, Stn Biol Roscoff,CS 90074, F-29688 Roscoff, France; [Deniaud-Bouet, Estelle; Michel, Gurvan; Tonon, Thierry; Kloareg, Bernard; Herve, Cecile] CNRS, Stn Biol Roscoff, UMR Integrat Biol Marine Models 8227, CS 90074, F-29688 Roscoff, France; [Kervarec, Nelly] Univ Bretagne Occidentale, Serv RMN RPE, UFR Sci & Tech, F-29200 Brest, France	Herve, C (corresponding author), Univ Paris 06, Sorbonne Univ, UMR Integrat Biol Marine Models 8227, Stn Biol Roscoff,CS 90074, F-29688 Roscoff, France.	cecile.herve@sb-roscoff.fr	Tonon, Thierry/A-3214-2009	Tonon, Thierry/0000-0002-1454-6018; MICHEL, Gurvan/0000-0002-3009-6205; Herve, Cecile/0000-0001-6649-8137			Albuquerque IRL, 2004, BRAZ J MED BIOL RES, V37, P167, DOI 10.1590/S0100-879X2004000200002; Ale MT, 2011, MAR DRUGS, V9, P2106, DOI 10.3390/md9102106; Anastyuk SD, 2010, CARBOHYD RES, V345, P2206, DOI 10.1016/j.carres.2010.07.043; Andersen RA, 2004, AM J BOT, V91, P1508, DOI 10.3732/ajb.91.10.1508; Arun A, 2013, NEW PHYTOL, V197, P503, DOI 10.1111/nph.12007; BABA M, 1988, ANTIVIR RES, V9, P335, DOI 10.1016/0166-3542(88)90035-6; Belanger KD, 2000, CURR OPIN PLANT BIOL, V3, P67, DOI 10.1016/S1369-5266(99)00043-6; BERGER F, 1994, SCIENCE, V263, P1421, DOI 10.1126/science.263.5152.1421; Berney C, 2006, P ROY SOC B-BIOL SCI, V273, P1867, DOI 10.1098/rspb.2006.3537; Bilan MI, 2010, CARBOHYD RES, V345, P2038, DOI 10.1016/j.carres.2010.07.009; Bilan MI, 2006, CARBOHYD RES, V341, P238, DOI 10.1016/j.carres.2005.11.009; Bilan MI, 2002, CARBOHYD RES, V337, P719, DOI 10.1016/S0008-6215(02)00053-8; Bitton R, 2006, MACROMOL BIOSCI, V6, P737, DOI 10.1002/mabi.200600073; Bouget FY, 1998, DEVELOPMENT, V125, P1999; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; Chevolot L, 2001, CARBOHYD RES, V330, P529, DOI 10.1016/S0008-6215(00)00314-1; Chizhov AO, 1999, CARBOHYD RES, V320, P108, DOI 10.1016/S0008-6215(99)00148-2; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Colin C, 2005, J BIOL INORG CHEM, V10, P156, DOI 10.1007/s00775-005-0626-8; Colin S, 2006, GLYCOBIOLOGY, V16, P1021, DOI 10.1093/glycob/cwl029; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; DISCHE Z, 1948, J BIOL CHEM, V175, P595; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Draget K. I., 2005, Polysaccharides and polyamides in the food industry: properties, production, and patents, P1; Camara RBG, 2011, MAR DRUGS, V9, P124, DOI 10.3390/md9010124; HAUG A, 1974, CARBOHYD RES, V32, P217, DOI 10.1016/S0008-6215(00)82100-X; Hematy K, 2009, CURR OPIN PLANT BIOL, V12, P406, DOI 10.1016/j.pbi.2009.06.007; Heyraud A, 1996, CARBOHYD RES, V289, P11, DOI 10.1016/0008-6215(96)00060-2; JAQUES LB, 1968, CAN J PHYSIOL PHARM, V46, P351, DOI 10.1139/y68-055; KLOAREG B, 1981, PHYSIOL VEG, V19, P427; KLOAREG B, 1987, J EXP BOT, V38, P1652, DOI 10.1093/jxb/38.10.1652; KLOAREG B, 1986, INT J BIOL MACROMOL, V8, P380, DOI 10.1016/0141-8130(86)90060-7; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; KNUTSON CA, 1968, ANAL BIOCHEM, V24, P470, DOI 10.1016/0003-2697(68)90154-1; KROPF DL, 1988, SCIENCE, V239, P187, DOI 10.1126/science.3336780; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Leblanc C, 2006, BIOCHIMIE, V88, P1773, DOI 10.1016/j.biochi.2006.09.001; MABEAU S, 1987, J EXP BOT, V38, P1573, DOI 10.1093/jxb/38.9.1573; MABEAU S, 1990, PHYTOCHEMISTRY, V29, P2441, DOI 10.1016/0031-9422(90)85163-A; MAHONY MC, 1993, CONTRACEPTION, V48, P277, DOI 10.1016/0010-7824(93)90146-X; Marais MF, 2001, CARBOHYD RES, V336, P155, DOI 10.1016/S0008-6215(01)00257-9; Meslet-Cladiere L, 2013, PLANT CELL, V25, P3089, DOI 10.1105/tpc.113.111336; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; MIN H, 1986, ANAL BIOCHEM, V155, P275, DOI 10.1016/0003-2697(86)90437-9; Nagaoka M, 1999, GLYCOCONJUGATE J, V16, P19, DOI 10.1023/A:1006945618657; NISHINO T, 1994, CARBOHYD RES, V255, P213, DOI 10.1016/S0008-6215(00)90980-7; NISHINO T, 1991, CARBOHYD RES, V214, P193, DOI 10.1016/S0008-6215(00)90542-1; NISHINO T, 1991, THROMB RES, V64, P723, DOI 10.1016/0049-3848(91)90072-5; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Paciorek T, 2010, CURR OPIN PLANT BIOL, V13, P661, DOI 10.1016/j.pbi.2010.09.016; Pereira MS, 1999, J BIOL CHEM, V274, P7656, DOI 10.1074/jbc.274.12.7656; Ponce NMA, 2003, CARBOHYD RES, V338, P153, DOI 10.1016/S0008-6215(02)00403-2; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; QUATRANO RS, 1976, PLANT PHYSIOL, V58, P224, DOI 10.1104/pp.58.2.224; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Sakai T, 2003, MAR BIOTECHNOL, V5, P536, DOI 10.1007/s10126-002-0107-9; Salgado LT, 2009, J PHYCOL, V45, P193, DOI 10.1111/j.1529-8817.2008.00642.x; Schoenwaelder MEA, 2000, PLANT BIOLOGY, V2, P24, DOI 10.1055/s-2000-9178; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Singleton VL, 1999, METHOD ENZYMOL, V299, P152; Tonon T, 2008, J PHYCOL, V44, P1250, DOI 10.1111/j.1529-8817.2008.00580.x; Verhaeghe EF, 2008, J BIOL INORG CHEM, V13, P257, DOI 10.1007/s00775-007-0319-6; Vreeland V, 1998, J PHYCOL, V34, P1, DOI 10.1046/j.1529-8817.1998.340001.x; Yamasaki-Miyamoto Y, 2009, J AGR FOOD CHEM, V57, P8677, DOI 10.1021/jf9010406; ZABLACKIS E, 1990, BOT MAR, V33, P273, DOI 10.1515/botm.1990.33.3.273; Zhu ZX, 2010, THROMB RES, V125, P419, DOI 10.1016/j.thromres.2010.02.011	69	120	125	1	120	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0305-7364	1095-8290		ANN BOT-LONDON	Ann. Bot.	OCT	2014	114	6			SI		1203	1216		10.1093/aob/mcu096			14	Plant Sciences	Plant Sciences	AS6FJ	WOS:000344359600014	24875633	Bronze, Green Published	Y	N	2021-04-07	
J	Tesson, B; Charrier, B				Tesson, Benoit; Charrier, Benedicte			Brown algal rnorphogenesis: atomic force microscopy as a tool to study the role of mechanical forces	FRONTIERS IN PLANT SCIENCE			English	Article						brown algae; Ectocarpus siliculosus; morphogenesis; cell wall; mechanical forces; AFM	SHEAR-STRESS; STIFFNESS TOMOGRAPHY; POLLEN-TUBE; CELL; MORPHOGENESIS; MORPHOLOGY; PHAEOPHYCEAE; CYTOSKELETON; ARCHITECTURE; METABOLISM	Over the last few years, a growing interest has been directed toward the use of macroalgae as a source of energy, food and molecules for the cosmetic and pharmaceutical industries. Besides this, macroalgal development remains poorly understood compared to other multicellular organisms. Brown algae (Phaeophyceae) form a monophyletic lineage of usually large multicellular algae which evolved independently from land plants. In their environment, they are subjected to strong mechanical forces (current, waves, and tide), in response to which they modify rapidly and reversibly their morphology. Because of their specific cellular features (cell wall composition, cytoskeleton organization), deciphering how they cope with these forces might help discover new control mechanisms of cell wall softening and cellulose synthesis. Despite the current scarcity in knowledge on brown algal cell wall dynamics and protein composition, we will illustrate, in the light of methods adapted to Ectocarpus siliculosus, to what extent atomic force microscopy can contribute to advance this field of investigation.	[Tesson, Benoit] Univ Calif San Diego, Scripps Inst Oceanog, Marine Biol Res Div, La Jolla, CA 92093 USA; [Charrier, Benedicte] Ctr Natl Rech Sci, Stn Biol Roscoff, Unites Mixtes Rech Integrat Biol Marine Models 82, Roscoff, France; [Charrier, Benedicte] Univ Paris 06, Sorbonne Univ, Unites Mixtes Rech Integrat Biol Marine Models 82, Roscoff, France	Tesson, B (corresponding author), Univ Calif San Diego, Scripps Inst Oceanog, Marine Biol Res Div, 9500 Gilman Dr, La Jolla, CA 92093 USA.	tessonben@gmail.com; benedicte.charrier@sb-roscoff.fr		Charrier, Benedicte/0000-0001-5721-1640			Blanchette CA, 1997, ECOLOGY, V78, P1563; Boller ML, 2006, J EXP BIOL, V209, P1894, DOI 10.1242/jeb.02225; Braam J, 2005, NEW PHYTOL, V165, P373, DOI 10.1111/j.1469-8137.2004.01263.x; Castle ES, 1937, J CELL COMPAR PHYSL, V10, P113, DOI 10.1002/jcp.1030100110; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Dardik A, 2005, J VASC SURG, V41, P869, DOI 10.1016/j.jvs.2005.01.020; De Martino A, 2009, BIOESSAYS, V31, P874, DOI 10.1002/bies.200900007; Deniaud-Bouet E, 2014, ANN BOT-LONDON, V114, P1203, DOI 10.1093/aob/mcu096; Denny M, 2002, J EXP BIOL, V205, P1355; Draget KI, 2011, FOOD HYDROCOLLOID, V25, P251, DOI 10.1016/j.foodhyd.2009.10.007; DUDGEON SR, 1992, J EXP MAR BIOL ECOL, V165, P23, DOI 10.1016/0022-0981(92)90287-K; Fernandes AN, 2012, PHYS REV E, V85, DOI 10.1103/PhysRevE.85.021916; Forouzesh E, 2013, PLANT J, V73, P509, DOI 10.1111/tpj.12042; Fowler-Walker MJ, 2006, MAR BIOL, V148, P755, DOI 10.1007/s00227-005-0125-z; Francius G, 2008, ENVIRON MICROBIOL, V10, P1344, DOI 10.1111/j.1462-2920.2007.01551.x; FRIEDLAND MT, 1995, J EXP MAR BIOL ECOL, V190, P109, DOI 10.1016/0022-0981(95)00038-S; Hamant O, 2013, CURR OPIN PLANT BIOL, V16, P654, DOI 10.1016/j.pbi.2013.06.006; Hamant O, 2010, NEW PHYTOL, V185, P369, DOI 10.1111/j.1469-8137.2009.03100.x; Hayot CM, 2012, J EXP BOT, V63, P2525, DOI 10.1093/jxb/err428; Hertz H., 2006, THE J, V1882, P156, DOI DOI 10.1515/CRLL.1882.92.156; Jaffe MJ, 2002, AM J BOT, V89, P375, DOI 10.3732/ajb.89.3.375; Karyophyllis D, 2000, EUR J PHYCOL, V35, P195, DOI 10.1017/S0967026200002717; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; Katsaros C, 2003, CELL BIOL INT, V27, P209, DOI 10.1016/S1065-6995(02)00312-8; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Koehl MAR, 2008, INTEGR COMP BIOL, V48, P834, DOI 10.1093/icb/icn069; KOEHL MAR, 1984, AM ZOOL, V24, P57; Lamport DTA, 2011, PLANT PHYSIOL, V156, P11, DOI 10.1104/pp.110.169011; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail Aude, 2013, Methods Mol Biol, V959, P323, DOI 10.1007/978-1-62703-221-6_22; Le Bail A, 2010, PLANT PHYSIOL, V153, P128, DOI 10.1104/pp.109.149708; Marga F, 2005, PLANT J, V43, P181, DOI 10.1111/j.1365-313X.2005.02447.x; Martone PT, 2012, AM J BOT, V99, P806, DOI 10.3732/ajb.1100541; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Milani P, 2013, J EXP BOT, V64, P4651, DOI 10.1093/jxb/ert169; Milani P, 2011, PLANT J, V67, P1116, DOI 10.1111/j.1365-313X.2011.04649.x; Mirabet V, 2011, ANNU REV PLANT BIOL, V62, P365, DOI 10.1146/annurev-arplant-042110-103852; Monshausen GB, 2009, TRENDS CELL BIOL, V19, P228, DOI 10.1016/j.tcb.2009.02.005; Muller DJ, 2011, TRENDS CELL BIOL, V21, P461, DOI 10.1016/j.tcb.2011.04.008; Parker A, 2001, J BIOL CHEM, V276, P5547, DOI 10.1074/jbc.M008463200; Peaucelle A, 2011, CURR BIOL, V21, P1720, DOI 10.1016/j.cub.2011.08.057; Radotic K, 2012, BIOPHYS J, V103, P386, DOI 10.1016/j.bpj.2012.06.046; Roduit C, 2009, BIOPHYS J, V97, P674, DOI 10.1016/j.bpj.2009.05.010; Rounds CM, 2013, ANNU REV PLANT BIOL, V64, P243, DOI 10.1146/annurev-arplant-050312-120150; Routier-Kierzkowska AL, 2013, CURR OPIN PLANT BIOL, V16, P25, DOI 10.1016/j.pbi.2012.11.002; Sneddon IN., 1965, INT J ENG SCI, V3, P47, DOI [10.1016/0020-7225(65)90019-4, DOI 10.1016/0020-7225(65)90019-4]; Stewart HL, 2006, MAR BIOL, V149, P721, DOI 10.1007/s00227-005-0186-z; Sumpio BE, 2005, J BIOL CHEM, V280, P11185, DOI 10.1074/jbc.M414631200; Takada Y, 2007, GENOME BIOL, V8, DOI 10.1186/gb-2007-8-5-215; Tesson B, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0061675; Tesson B, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0014300; Tesson B, 2010, J STRUCT BIOL, V169, P62, DOI 10.1016/j.jsb.2009.08.013; Vogler H, 2013, PLANT J, V73, P617, DOI 10.1111/tpj.12061; Wu JZ, 2008, J EXP BOT, V59, P2529, DOI 10.1093/jxb/ern119; Wyatt HDM, 2008, BOTANY, V86, P385, DOI 10.1139/B08-003; Yun SS, 2002, J BIOL CHEM, V277, P34808, DOI 10.1074/jbc.M205417200	57	7	7	0	28	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND	1664-462X			FRONT PLANT SCI	Front. Plant Sci.	SEP 17	2014	5								471	10.3389/fpls.2014.00471			7	Plant Sciences	Plant Sciences	AR8ZC	WOS:000343858800001	25278949	DOAJ Gold, Green Published			2021-04-07	
J	Ahmed, S; Cock, JM; Pessia, E; Luthringer, R; Cormier, A; Robuchon, M; Sterck, L; Peters, AF; Dittami, SM; Corre, E; Valero, M; Aury, JM; Roze, D; Van de Peer, Y; Bothwell, J; Marais, GAB; Coelho, SM				Ahmed, Sophia; Cock, J. Mark; Pessia, Eugenie; Luthringer, Remy; Cormier, Alexandre; Robuchon, Marine; Sterck, Lieven; Peters, Akira F.; Dittami, Simon M.; Corre, Erwan; Valero, Myriam; Aury, Jean-Marc; Roze, Denis; Van de Peer, Yves; Bothwell, John; Marais, Gabriel A. B.; Coelho, Susana M.			A Haploid System of Sex Determination in the Brown Alga Ectocarpus sp.	CURRENT BIOLOGY			English	Article							Y-CHROMOSOME; CODON USAGE; EXPRESSION ANALYSIS; GENE CONVERSION; EVOLUTION; GENOME; SEQUENCES; TRANSCRIPTOME; MULTIPLE; IDENTIFICATION	Background: A common feature of most genetic sex-determination systems studied so far is that sex is determined by nonrecombining genomic regions, which can be of various sizes depending on the species. These regions have evolved independently and repeatedly across diverse groups. A number of such sex-determining regions (SDRs) have been studied in animals, plants, and fungi, but very little is known about the evolution of sexes in other eukaryotic lineages. Results: We report here the sequencing and genomic analysis of the SDR of Ectocarpus, a brown alga that has been evolving independently from plants, animals, and fungi for over one giga-annum. In Ectocarpus, sex is expressed during the haploid phase of the life cycle, and both the female (U) and the male (V) sex chromosomes contain nonrecombining regions. The U and V of this species have been diverging for more than 70 mega-annum, yet gene degeneration has been modest, and the SDR is relatively small, with no evidence for evolutionary strata. These features may be explained by the occurrence of strong purifying selection during the haploid phase of the life cycle and the low level of sexual dimorphism. V is dominant over U, suggesting that femaleness may be the default state, adopted when the male haplotype is absent. Conclusions: The Ectocarpus UV system has clearly had a distinct evolutionary trajectory not only to the well-studied XY and ZW systems but also to the UV systems described so far. Nonetheless, some striking similarities exist, indicating remarkable universality of the underlying processes shaping sex chromosome evolution across distant lineages.	[Ahmed, Sophia; Cock, J. Mark; Luthringer, Remy; Cormier, Alexandre; Robuchon, Marine; Dittami, Simon M.; Coelho, Susana M.] Univ Paris 06, Sorbonne Univ, CNRS, UMR 8227,Stn Biol Roscoff,CS 90074, F-29688 Roscoff, France; [Ahmed, Sophia; Bothwell, John] Queens Univ Belfast, Ctr Med Biol, Belfast BT9 7BL, Antrim, North Ireland; [Pessia, Eugenie; Marais, Gabriel A. B.] Univ Lyon 1, CNRS, UMR 5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne, France; [Sterck, Lieven; Van de Peer, Yves] Univ Ghent, Dept Plant Syst Biol VIB, B-9052 Ghent, Belgium; [Sterck, Lieven; Van de Peer, Yves] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France; [Aury, Jean-Marc] CEA, IG, F-91000 Evry, France; [Corre, Erwan] ABiMS Platform, FR2424, Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France; [Robuchon, Marine; Valero, Myriam; Roze, Denis] UPMC, Sorbonne Univ, CNRS, UMI 3604,PUCCh,UACH,Stn Biol Roscoff,CS 90074, F-29688 Roscoff, France; [Van de Peer, Yves] Univ Pretoria, Genom Res Inst, ZA-0028 Pretoria, South Africa	Coelho, SM (corresponding author), Univ Paris 06, Sorbonne Univ, CNRS, UMR 8227,Stn Biol Roscoff,CS 90074, F-29688 Roscoff, France.	coelho@sb-roscoff.fr	Valero, Myriam/C-7550-2011; Sterck, Lieven/A-9439-2016; Valero, Myriam/M-6052-2019; Van de Peer, Yves/AAE-7666-2019; corre, erwan/O-4669-2019; Aury, Jean-Marc/N-1621-2019; Van de Peer, Yves/D-4388-2009; Coelho, Susana/ABH-8166-2020; Robuchon, Marine/D-4019-2018	Valero, Myriam/0000-0002-9000-1423; Sterck, Lieven/0000-0001-7116-4000; Valero, Myriam/0000-0002-9000-1423; Van de Peer, Yves/0000-0003-4327-3730; corre, erwan/0000-0001-6354-2278; Aury, Jean-Marc/0000-0003-1718-3010; Van de Peer, Yves/0000-0003-4327-3730; Robuchon, Marine/0000-0001-5873-2915; Cock, J. Mark/0000-0002-2650-0383; Cormier, Alexandre/0000-0002-7775-8413	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Agence Nationale de la Recherche (project SEXSEAWEED)French National Research Agency (ANR); University Pierre and Marie Curie (Emergence program); Interreg program France (Channel) - England (project Marinexus); Interreg IVB (project EnAlgae)	The authors wish to thank Thomas Broquet, Veronique Storm, and Sylvain Mousset for advice on the statistical analysis; Aurelie Kapusta for help with Repclass; Emmanuelle Lerat for explanations about TE librairies; Thomas Bigot and Florent Lassalle for help with TPMS; Catherine Leblanc, Florian Weinberger, Gareth Pearson, and Olivier de Clerk for sharing unpublished RNA-seq data; and Helen Skaletsky for help with intrachromosomal similarity analyses. This work was supported by the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (project SEXSEAWEED), the University Pierre and Marie Curie (Emergence program), the Interreg program France (Channel) - England (project Marinexus), and the Interreg IVB (project EnAlgae).	Allen CE, 1935, BOT REV, V1, P269; Anders S, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-10-r106; Assis R, 2012, GENOME BIOL EVOL, V4, P1189, DOI 10.1093/gbe/evs093; Bachtrog D, 2003, NAT GENET, V34, P215, DOI 10.1038/ng1164; Bachtrog D, 2008, GENETICS, V179, P1513, DOI 10.1534/genetics.107.084012; Bachtrog D, 2013, NAT REV GENET, V14, P113, DOI 10.1038/nrg3366; Bachtrog D, 2011, CURR BIOL, V21, pR685, DOI 10.1016/j.cub.2011.08.027; Bachtrog D, 2011, TRENDS GENET, V27, P350, DOI 10.1016/j.tig.2011.05.005; Bartolome C, 2006, GENETICS, V174, P2033, DOI 10.1534/genetics.106.064113; Bergero R, 2011, CURR BIOL, V21, P1470, DOI 10.1016/j.cub.2011.07.032; Bergero R, 2009, TRENDS ECOL EVOL, V24, P94, DOI 10.1016/j.tree.2008.09.010; Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; Billiard S, 2011, BIOL REV, V86, P421, DOI 10.1111/j.1469-185X.2010.00153.x; Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; BULL JJ, 1978, AM NAT, V112, P245, DOI 10.1086/283267; Bull JJ., 1983, EVOLUTION SEX DETERM; Carvalho AB, 2013, GENOME RES, V23, P1894, DOI 10.1101/gr.156034.113; Castresana J, 2000, MOL BIOL EVOL, V17, P540, DOI 10.1093/oxfordjournals.molbev.a026334; Charif D, 2005, BIOINFORMATICS, V21, P545, DOI 10.1093/bioinformatics/bti037; Charlesworth B, 2000, PHILOS T R SOC B, V355, P1563, DOI 10.1098/rstb.2000.0717; Charlesworth D, 2005, HEREDITY, V95, P118, DOI 10.1038/sj.hdy.6800697; Chibalina MV, 2011, CURR BIOL, V21, P1475, DOI 10.1016/j.cub.2011.07.045; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Degroeve S, 2005, BIOINFORMATICS, V21, P1332, DOI 10.1093/bioinformatics/bti166; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Ellegren H, 2001, GENETICS, V158, P325; Ellegren H, 2007, NAT REV GENET, V8, P689, DOI 10.1038/nrg2167; EVANS LV, 1963, NATURE, V198, P215, DOI 10.1038/198215a0; Ferris P, 2010, SCIENCE, V328, P351, DOI 10.1126/science.1186222; Feschotte C, 2009, GENOME BIOL EVOL, V1, P205, DOI 10.1093/gbe/evp023; Flutre T, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0016526; Foissac S, 2008, CURR BIOINFORM, V3, P87, DOI 10.2174/157489308784340702; FOSTER JW, 1992, NATURE, V359, P531, DOI 10.1038/359531a0; Fry JD, 2010, EVOLUTION, V64, P1510, DOI 10.1111/j.1558-5646.2009.00898.x; Gouy M, 2010, MOL BIOL EVOL, V27, P221, DOI 10.1093/molbev/msp259; Grabherr MG, 2011, NAT BIOTECHNOL, V29, P644, DOI 10.1038/nbt.1883; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; HILL WG, 1966, GENET RES, V8, P269, DOI 10.1017/S0016672300010156; Hood ME, 2013, GENETICS, V193, P309, DOI 10.1534/genetics.112.146266; Idnurm A, 2008, NATURE, V451, P193, DOI 10.1038/nature06453; Ironside JE, 2010, BIOESSAYS, V32, P718, DOI 10.1002/bies.200900124; Jordan CY, 2012, EVOLUTION, V66, P505, DOI 10.1111/j.1558-5646.2011.01448.x; Lahn BT, 1999, SCIENCE, V286, P964, DOI 10.1126/science.286.5441.964; Lemaitre C, 2009, GENOME BIOL EVOL, V1, P56, DOI 10.1093/gbe/evp006; Lewis RJ, 1996, PHYCOLOGIA, V35, P19, DOI 10.2216/i0031-8884-35-1-19.1; Loytynoja A, 2005, P NATL ACAD SCI USA, V102, P10557, DOI 10.1073/pnas.0409137102; McDaniel SF, 2013, EVOLUTION, V67, P2811, DOI 10.1111/evo.12165; Menkis A, 2008, PLOS GENET, V4, DOI 10.1371/journal.pgen.1000030; Muller DG, 1975, LYNGB ARCH PROTISTEN, V117, P297; Penel S, 2009, BMC BIOINFORMATICS, V10, DOI 10.1186/1471-2105-10-S6-S3; Pessia E, 2012, GENOME BIOL EVOL, V4, P787, DOI 10.1093/gbe/evs052; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Potrzebowski L, 2008, PLOS BIOL, V6, P709, DOI 10.1371/journal.pbio.0060080; Qiu S, 2013, GENETICS, V194, P663, DOI 10.1534/genetics.113.152397; RICE WR, 1984, EVOLUTION, V38, P735, DOI [10.2307/2408385, 10.1111/j.1558-5646.1984.tb00346.x]; Rozen S, 2003, NATURE, V423, P873, DOI 10.1038/nature01723; Schuler GD, 1997, GENOME RES, V7, P541, DOI 10.1101/gr.7.5.541; SHARP PM, 1987, NUCLEIC ACIDS RES, V15, P1281, DOI 10.1093/nar/15.3.1281; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Skaletsky H, 2003, NATURE, V423, P825, DOI 10.1038/nature01722; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Stock M, 2011, PLOS BIOL, V9, DOI 10.1371/journal.pbio.1001062; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Trapnell C, 2012, NAT PROTOC, V7, P562, DOI 10.1038/nprot.2012.016; Trapnell C, 2010, NAT BIOTECHNOL, V28, P511, DOI 10.1038/nbt.1621; Trapnell C, 2009, BIOINFORMATICS, V25, P1105, DOI 10.1093/bioinformatics/btp120; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; Veyrunes F, 2008, GENOME RES, V18, P965, DOI 10.1101/gr.7101908; Vicoso B, 2013, P NATL ACAD SCI USA, V110, P6453, DOI 10.1073/pnas.1217027110; Wang JP, 2012, P NATL ACAD SCI USA, V109, P13710, DOI 10.1073/pnas.1207833109; Yamato KT, 2007, P NATL ACAD SCI USA, V104, P6472, DOI 10.1073/pnas.0609054104; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075	75	65	65	0	84	CELL PRESS	CAMBRIDGE	50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA	0960-9822	1879-0445		CURR BIOL	Curr. Biol.	SEP 8	2014	24	17					1945	1957		10.1016/j.cub.2014.07.042			13	Biochemistry & Molecular Biology; Biology; Cell Biology	Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology	AO7OA	WOS:000341541300017	25176635				2021-04-07	
J	Fu, G; Nagasato, C; Oka, S; Cock, JM; Motomura, T				Fu, Gang; Nagasato, Chikako; Oka, Seiko; Cock, J. Mark; Motomura, Taizo			Proteomics Analysis of Heterogeneous Flagella in Brown Algae (Stramenopiles)	PROTIST			English	Article						Blue light receptor; brown algae; creatine kinase; flagella; phototaxis; proteomics	SCYTOSIPHON-LOMENTARIA SCYTOSIPHONALES; ECTOCARPUS-SILICULOSUS ECTOCARPALES; EUKARYOTIC FLAGELLUM; PHOTOTACTIC RESPONSES; MOLECULAR CHAPERONE; BINDING-PROTEINS; PRIMARY CILIUM; FINE-STRUCTURE; MALE GAMETE; BASAL BODY	Flagella are conserved organelles among eukaryotes and they are composed of many proteins, which are necessary for flagellar assembly, maintenance and function. Stramenopiles, which include brown algae, diatoms and oomycetes, possess two laterally inserted flagella. The anterior flagellum (AF) extends forward and bears tripartite mastigonemes, whilst the smooth posterior flagellum (PF) often has a paraflagellar body structure. These heterogeneous flagella have served as crucial structures in algal studies especially from a viewpoint of phylogeny. However, the protein compositions of the flagella are still largely unknown. Here we report a LC-MS/MS based proteomics analysis of brown algal flagella. In total, 495 flagellar proteins were identified. Functional annotation of the proteome data revealed that brown algal flagellar proteins were associated with cell motility, signal transduction and various metabolic activities. We separately isolated AF and PF and analyzed their protein compositions. This analysis led to the identification of several AF- and PF-specific proteins. Among the PF-specific proteins, we found a candidate novel blue light receptor protein involved in phototaxis, and named it HELMCHROME because of the steering function of PF. Immunological analysis revealed that this protein was localized along the whole length of the PF and concentrated in the paraflagellar body. (C) 2014 Elsevier GmbH. All rights reserved.	[Fu, Gang; Nagasato, Chikako; Motomura, Taizo] Hokkaido Univ, Field Sci Ctr Northern Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510013, Japan; [Oka, Seiko] Hokkaido Univ, Creat Res Inst, Instrumental Anal Div, Equipment Management Ctr, Sapporo, Hokkaido 0010021, Japan; [Cock, J. Mark] Univ Paris 06, F-29682 Roscoff, France; [Cock, J. Mark] CNRS, Unite Mixte Rech 7139, Stn Biol Roscoff, Lab Int Associe Dispersal & Adaptat Marine Specie, F-29682 Roscoff, France	Motomura, T (corresponding author), Hokkaido Univ, Field Sci Ctr Northern Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510013, Japan.	motomura@fsc.hokudai.ac.jp		Cock, J. Mark/0000-0002-2650-0383	Ministry of Education, Culture, Sports, Science and Technology of JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT) [24112701]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [26440160] Funding Source: KAKEN	We would like to thank Dr. Kazuo Inaba, Shimoda Marine Research Center, Tsukuba University, for his helpful advice on the flagellar proteomics analysis. This study was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (24112701).	Avidor-Reiss T, 2004, CELL, V117, P527, DOI 10.1016/S0092-8674(04)00412-X; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Baron DM, 2007, J CELL SCI, V120, P478, DOI 10.1242/jcs.03352; Bisgrove BW, 2006, DEVELOPMENT, V133, P4131, DOI 10.1242/dev.02595; BLOCH MA, 1995, J CELL SCI, V108, P3541; Broadhead R, 2006, NATURE, V440, P224, DOI 10.1038/nature04541; Carvalho-Santos Z, 2011, J CELL BIOL, V194, P165, DOI 10.1083/jcb.201011152; Cavalier-Smith T, 2002, INT J SYST EVOL MICR, V52, P297, DOI 10.1099/00207713-52-2-297; CLAYTON MN, 1989, SYST ASSOC SPEC VOL, V38, P229; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Crosson S, 2001, P NATL ACAD SCI USA, V98, P2995, DOI 10.1073/pnas.051520298; Crosson S, 2003, BIOCHEMISTRY-US, V42, P2, DOI 10.1021/bi026978l; Davenport JR, 2005, AM J PHYSIOL-RENAL, V289, pF1159, DOI 10.1152/ajprenal.00118.2005; Davy BE, 2003, HUM MOL GENET, V12, P1163, DOI 10.1093/hmg/ddg122; de Groot PWJ, 2008, MOL MICROBIOL, V68, P535, DOI 10.1111/j.1365-2958.2008.06182.x; Elias M, 2009, GENE, V442, P63, DOI 10.1016/j.gene.2009.04.011; Fan YL, 2004, NAT GENET, V36, P989, DOI 10.1038/ng1414; Feng YY, 2004, NAT CELL BIOL, V6, P1034, DOI 10.1038/ncb1104-1034; Fliegauf M, 2007, NAT REV MOL CELL BIO, V8, P880, DOI 10.1038/nrm2278; Flores-Moya A, 2002, J PHOTOCH PHOTOBIO B, V66, P134, DOI 10.1016/S1011-1344(02)00233-6; Fu G, 2013, PROTOPLASMA, V250, P261, DOI 10.1007/s00709-012-0405-7; Fujita S, 2005, EUR J PHYCOL, V40, P159, DOI 10.1080/09670260500063193; Geer LY, 2002, GENOME RES, V12, P1619, DOI 10.1101/gr.278202; GELLER A, 1981, J EXP BIOL, V92, P53; Gherman A, 2006, NAT GENET, V38, P961, DOI 10.1038/ng0906-961; Ginger ML, 2008, NAT REV MICROBIOL, V6, P838, DOI 10.1038/nrmicro2009; Govorunova EG, 2004, BIOPHYS J, V86, P2342, DOI 10.1016/S0006-3495(04)74291-5; Guiry M.D., 2014, ALGAEBASE; Henry EC, 1982, 1 ZOOSPORES J PHYCOL, V18, P550; Henry EC, 1982, 2 SPERMS J PHYCOL, V18, P570; Idei M, 2013, PROTOPLASMA, V250, P833, DOI 10.1007/s00709-012-0465-8; Inglis PN, 2006, TRENDS GENET, V22, P491, DOI 10.1016/j.tig.2006.07.006; Judelson HS, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0047624; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; Kawai H, 1996, PROTOPLASMA, V191, P172, DOI 10.1007/BF01281815; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KAWAI H, 1988, J PHYCOL, V24, P114; KAWAI H, 1992, BOT MAG TOKYO, V105, P171, DOI 10.1007/BF02489413; Koide A, 1998, J MOL BIOL, V284, P1141, DOI 10.1006/jmbi.1998.2238; KOZMINSKI KG, 1993, P NATL ACAD SCI USA, V90, P5519, DOI 10.1073/pnas.90.12.5519; Lechtreck KF, 2007, J CELL BIOL, V176, P473, DOI 10.1083/jcb.200611115; Li JB, 2004, CELL, V117, P541, DOI 10.1016/S0092-8674(04)00450-7; Lorenzetti D, 2004, P NATL ACAD SCI USA, V101, P8402, DOI 10.1073/pnas.0401832101; Losi A, 2012, ANNU REV PLANT BIOL, V63, P49, DOI 10.1146/annurev-arplant-042811-105538; Mahjoub MR, 2005, J AM SOC NEPHROL, V16, P3485, DOI 10.1681/ASN.2005080824; Maier I, 1997, EUR J PHYCOL, V32, P255; Maier I, 1997, EUR J PHYCOL, V32, P241; MANTON I, 1953, J EXP BOT, V4, P319, DOI 10.1093/jxb/4.3.319; MANTON I, 1951, J EXP BOT, V2, P242, DOI 10.1093/jxb/2.2.242; Marshall WF, 2008, J CELL BIOL, V180, P17, DOI 10.1083/jcb.200710085; Marshall WF, 2006, CURR BIOL, V16, pR604, DOI 10.1016/j.cub.2006.07.012; Matsunaga S, 2010, PHOTOCHEM PHOTOBIOL, V86, P374, DOI 10.1111/j.1751-1097.2009.00676.x; Mitchell BF, 2005, MOL BIOL CELL, V16, P4509, DOI 10.1091/mbc.E05-04-0347; Mitchell DR, 2007, ADV EXP MED BIOL, V607, P130; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; Nagarkatti-Gude DR, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0020625; Nagasato C, 2002, J CELL SCI, V115, P2541; Nicastro D, 2005, P NATL ACAD SCI USA, V102, P15889, DOI 10.1073/pnas.0508274102; OKELLY CJ, 1989, SYST ASSOC SPEC VOL, V38, P255; Okita N, 2005, J CELL SCI, V118, P529, DOI 10.1242/jcs.01633; OSBORN M, 1982, METHOD CELL BIOL, V24, P97; Pazour GJ, 2005, J CELL BIOL, V170, P103, DOI 10.1083/jcb.200504008; Pazour GJ, 2008, CURR TOP DEV BIOL, V85, P115, DOI 10.1016/S0070-2153(08)00805-3; Provasoli L., 1968, CULTURES COLLECTIONS, P63; Ralston KS, 2009, ANNU REV MICROBIOL, V63, P335, DOI 10.1146/annurev.micro.091208.073353; Rosenbaum JL, 2002, NAT REV MOL CELL BIO, V3, P813, DOI 10.1038/nrm952; Shapiro J, 2005, EUKARYOT CELL, V4, P1591, DOI 10.1128/EC.4.9.1591-1594.2005; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Stephens RE, 1999, CELL MOTIL CYTOSKEL, V44, P274, DOI 10.1002/(SICI)1097-0169(199912)44:4<274::AID-CM5>3.0.CO;2-O; TOMBES RM, 1987, BIOPHYS J, V52, P75, DOI 10.1016/S0006-3495(87)83190-9; Tombes RM, 1985, CELL, V41, P325; Tuson M, 2011, DEVELOPMENT, V138, P4921, DOI 10.1242/dev.070805; Varmuza S, 1999, DEV BIOL, V205, P98, DOI 10.1006/dbio.1998.9100; Woodland HR, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003778; WOTHE DD, 1990, P NATL ACAD SCI USA, V87, P5203, DOI 10.1073/pnas.87.13.5203; Zhang ZB, 2004, P NATL ACAD SCI USA, V101, P12946, DOI 10.1073/pnas.0404280101; Zhang ZB, 2002, MOL CELL BIOL, V22, P7993, DOI 10.1128/MCB.22.22.7993-8004.2002	77	25	25	1	19	ELSEVIER GMBH, URBAN & FISCHER VERLAG	JENA	OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY	1434-4610			PROTIST	Protist	SEP	2014	165	5					662	675		10.1016/j.protis.2014.07.007			14	Microbiology	Microbiology	AS4IC	WOS:000344236600007	25150613	Green Published			2021-04-07	
J	Kupper, FC; Leblanc, C; Meyer-Klaucke, W; Potin, P; Feiters, MC				Kuepper, Frithjof C.; Leblanc, Catherine; Meyer-Klaucke, Wolfram; Potin, Philippe; Feiters, Martin C.			DIFFERENT SPECIATION FOR BROMINE IN BROWN AND RED ALGAE, REVEALED BY IN VIVO X-RAY ABSORPTION SPECTROSCOPIC STUDIES	JOURNAL OF PHYCOLOGY			English	Article						brown algae; extended X-ray absorption fine structure; microalgae; red algae; X-ray absorption spectroscopy	MARINE NATURAL-PRODUCTS; PLASMA-MASS SPECTROMETRY; WEED LAMINARIA-DIGITATA; BIO-AVAILABLE IODINE; CURVED-WAVE THEORY; DELISEA-PULCHRA; SECONDARY METABOLITES; VESICLE CELLS; VANADIUM BROMOPEROXIDASE; ASPARAGOPSIS-TAXIFORMIS	Members of various algal lineages are known to be strong producers of atmospherically relevant halogen emissions, that is a consequence of their capability to store and metabolize halogens. This study uses a noninvasive, synchrotron-based technique, X-ray absorption spectroscopy, for addressing in vivo bromine speciation in the brown algae Ectocarpus siliculosus, Ascophyllum nodosum, and Fucus serratus, the red algae Gracilaria dura, G.gracilis, Chondrus crispus, Osmundea pinnatifida, Asparagopsis armata, Polysiphonia elongata, and Corallina officinalis, the diatom Thalassiosira rotula, the dinoflagellate Lingulodinium polyedrum and a natural phytoplankton sample. The results highlight a diversity of fundamentally different bromine storage modes: while most of the stramenopile representatives and the dinoflagellate store mostly bromide, there is evidence for Br incorporated in nonaromatic hydrocarbons in Thalassiosira. Red algae operate various organic bromine stores - including a possible precursor (by the haloform reaction) for bromoform in Asparagopsis and aromatically bound Br in Polysiphonia and Corallina. Large fractions of the bromine in the red algae G.dura and C.crispus and the brown alga F.serratus are present as Br- defects in solid KCl, similar to what was reported earlier for Laminaria parts. These results are discussed according to different defensive strategies that are used within algal taxa to cope with biotic or abiotic stresses.	[Kuepper, Frithjof C.] Univ Aberdeen, Oceanlab, Newburgh AB41 6 AA, Aberdeen, Scotland; [Kuepper, Frithjof C.] Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland; [Leblanc, Catherine; Potin, Philippe] CNRS, Biol Stn, F-29680 Roscoff, France; [Leblanc, Catherine; Potin, Philippe] Univ Paris 06, Biol Stn, Marine Plants & Biomol Lab, F-29680 Roscoff, France; [Meyer-Klaucke, Wolfram] DESY, EMBL, Hamburg Unit, D-22607 Hamburg, Germany; [Feiters, Martin C.] Radboud Univ Nijmegen, Dept Organ Chem, Inst Mol & Mat, NL-6525 AJ Nijmegen, Netherlands	Kupper, FC (corresponding author), Univ Aberdeen, Oceanlab, Main St, Newburgh AB41 6 AA, Aberdeen, Scotland.	fkuepper@abdn.ac.uk	/D-4640-2012; Meyer-Klaucke, Wolfram/G-1148-2010		Natural Environment Research Council (UK)UK Research & Innovation (UKRI)NERC Natural Environment Research Council [WP4.5]; French National and CEA Program; MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland); Scottish Funding Council; European Community in the framework of the Access to Research Infrastructure Action of the Improving Human Potential Program;  [HR09011]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [dml010007] Funding Source: researchfish	Funding from the Natural Environment Research Council (UK) through the Oceans 2025 (WP4.5) programs to FCK, the French National and CEA Program "Toxicologie Nucleaire Environnementale" to PP and CL, and the MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) is gratefully acknowledged. Furthermore, the authors are grateful for support from the European Community in the framework of the Access to Research Infrastructure Action of the Improving Human Potential Program to the EMBL Hamburg Outstation. Special thanks are due to Christine N. Campbell (CCAP, SAMS, Oban) for growing the two microalgal cultures. We are grateful to Johanna Fehling (Scottish Association for Marine Science) for providing the two microalgal cultures - she untimely passed away in early 2011.	Alicke B, 1999, NATURE, V397, P572, DOI 10.1038/17508; Balard AJ, 1826, ANN CHIM PHYS, V32, P337; BEISSNER RS, 1981, BIOCHEMISTRY-US, V20, P3724, DOI 10.1021/bi00516a009; Binsted N., 1991, EXCURV92 SERC DAR LA; Bottger LH, 2012, J EXP BOT, V63, P5763, DOI 10.1093/jxb/ers225; Butler A, 2004, NAT PROD REP, V21, P180, DOI 10.1039/b302337k; Butler A, 2009, NATURE, V460, P848, DOI 10.1038/nature08303; Cabrita MT, 2010, MAR DRUGS, V8, P2301, DOI 10.3390/md8082301; Carpenter LJ, 2000, GLOBAL BIOGEOCHEM CY, V14, P1191, DOI 10.1029/2000GB001257; Carpenter LJ, 2012, CHEM SOC REV, V41, P6473, DOI 10.1039/c2cs35121h; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; COURTOIS B, 1813, ANN CHIM, V88, P304; CRAIGIE JS, 1967, SCIENCE, V157, P1058, DOI 10.1126/science.157.3792.1058; Dangeard P, 1928, CR HEBD ACAD SCI, V186, P892; Davyt D, 2001, J NAT PROD, V64, P1552, DOI 10.1021/np0102307; Dworjanyn SA, 1999, MAR BIOL, V133, P727, DOI 10.1007/s002270050514; Erickson K. L., 1983, MARINE NATURAL PRODU, V3, pP131; Eschle NN, 1897, Z PHYSL CHEM, V23, P30; Faulkner DJ, 2001, NAT PROD REP, V18, P1, DOI 10.1039/b006897g; FAULKNER DJ, 1995, NAT PROD REP, V12, P223, DOI 10.1039/np9951200223; Feiters MC, 2005, J AM CHEM SOC, V127, P15340, DOI 10.1021/ja053416r; Feiters MC, 2005, J SYNCHROTRON RADIAT, V12, P85, DOI 10.1107/S0909049504027815; FENICAL WH, 1975, J PHYCOL, V11, P20; Gribble GW, 2003, CHEMOSPHERE, V52, P289, DOI 10.1016/S0045-6535(03)00207-8; Guillard R. L. L., 1975, CULTURE MARINE INVER, P29, DOI DOI 10.1007/978-1-4615-8714-9_3; GURMAN SJ, 1984, J PHYS C SOLID STATE, V17, P143, DOI 10.1088/0022-3719/17/1/019; GURMAN SJ, 1986, J PHYS C SOLID STATE, V19, P1845, DOI 10.1088/0022-3719/19/11/021; Harder T, 2012, J CHEM ECOL, V38, P442, DOI 10.1007/s10886-012-0119-5; Harper MK, 2001, CRC MAR SCI, P3; HERMES C, 1984, NUCL INSTRUM METH A, V222, P207, DOI 10.1016/0167-5087(84)90532-5; Hill RA, 2007, ANNU REP PROG CHEM B, V103, P125, DOI 10.1039/b614408j; Hou XL, 1998, SCI TOTAL ENVIRON, V222, P141, DOI 10.1016/S0048-9697(98)00299-X; Hughes C, 2013, MAR CHEM, V151, P35, DOI 10.1016/j.marchem.2013.01.007; Juagdan EG, 1997, TETRAHEDRON, V53, P521, DOI 10.1016/S0040-4020(96)01002-2; KATSUI N, 1967, TETRAHEDRON, V23, P1185, DOI 10.1016/0040-4020(67)85068-3; Keller D. K., 1987, J PHYCOL, V23, P633; Kilah NL, 2010, J AM CHEM SOC, V132, P11893, DOI 10.1021/ja105263q; Konig GM, 1997, J NAT PROD, V60, P967, DOI 10.1021/np970181r; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kupper FC, 2013, J EXP BOT, V64, P2653, DOI 10.1093/jxb/ert110; Kupper FC, 2011, ANGEW CHEM INT EDIT, V50, P11598, DOI 10.1002/anie.201100028; Kupper H, 2009, PLANT PHYSIOL, V151, P702, DOI 10.1104/pp.109.139717; Kupper FC, 1998, PLANTA, V207, P163, DOI 10.1007/s004250050469; Kupper H, 2004, PLANT PHYSIOL, V134, P748, DOI 10.1104/pp.103.032953; Kurata K, 1998, PHYTOCHEMISTRY, V47, P363, DOI 10.1016/S0031-9422(97)00461-5; Kylin H., 1929, H-S Z PHYSIOL CHEM, V186, P50; Lane AL, 2009, P NATL ACAD SCI USA, V106, P7314, DOI 10.1073/pnas.0812020106; LasBarre S., 2010, MAR DRUGS, V8, P988; Lee A, 2001, PLANTA, V213, P977, DOI 10.1007/s004250100574; LOVELOCK JE, 1975, NATURE, V256, P193, DOI 10.1038/256193a0; Manefield M, 2002, MICROBIOL-SGM, V148, P1119, DOI 10.1099/00221287-148-4-1119; Marshall RA, 2003, CHEMOSPHERE, V52, P471, DOI 10.1016/S0045-6535(03)00197-8; Mata L, 2011, J APPL PHYCOL, V23, P827, DOI 10.1007/s10811-010-9582-y; Maximilien R, 1998, AQUAT MICROB ECOL, V15, P233, DOI 10.3354/ame015233; MCCONNELL O, 1977, PHYTOCHEMISTRY, V16, P367, DOI 10.1016/0031-9422(77)80067-8; McKey D., 1979, DISTRIBUTION SECONDA, pP55; Metrangolo P, 2005, ACCOUNTS CHEM RES, V38, P386, DOI 10.1021/ar0400995; Mtolera MSP, 1996, EUR J PHYCOL, V31, P89, DOI 10.1080/09670269600651241; Neumann CS, 2008, CHEM BIOL, V15, P99, DOI 10.1016/j.chembiol.2008.01.006; Nolting H. F., 1992, EXPROG EXAFS DATA RE; Nyadong L, 2009, ANAL BIOANAL CHEM, V394, P245, DOI 10.1007/s00216-009-2674-3; O'Dowd CD, 2002, NATURE, V417, P632, DOI 10.1038/nature00775; Ohsawa N, 2001, PHYTOCHEMISTRY, V58, P683, DOI 10.1016/S0031-9422(01)00259-X; Paul C, 2011, NAT PROD REP, V28, P186, DOI 10.1039/c0np00043d; Paul NA, 2006, J PHYCOL, V42, P637, DOI 10.1111/j.1529-8817.2006.00226.x; Paul NA, 2006, MAR ECOL PROG SER, V306, P87, DOI 10.3354/meps306087; Paul NA, 2006, MAR ECOL PROG SER, V323, P1, DOI 10.3354/meps323001; Pedersen M, 1996, SCI MAR, V60, P257; PEDERSEN M, 1983, BOT MAR, V26, P113, DOI 10.1515/botm.1983.26.3.113; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; PETTIFER RF, 1985, J APPL CRYSTALLOGR, V18, P404, DOI 10.1107/S0021889885010627; Rasmussen TB, 2000, MICROBIOL-UK, V146, P3237, DOI 10.1099/00221287-146-12-3237; Romaris-Hortas V, 2012, ANAL CHIM ACTA, V745, P24, DOI 10.1016/j.aca.2012.07.035; Romaris-Hortas V, 2012, J CHROMATOGR A, V1236, P164, DOI 10.1016/j.chroma.2012.03.019; Scarratt MG, 1996, MAR CHEM, V54, P263, DOI 10.1016/0304-4203(96)00036-9; Schoenwaelder MEA, 2002, PHYCOLOGIA, V41, P125, DOI 10.2216/i0031-8884-41-2-125.1; SHAW TI, 1960, PROC R SOC SER B-BIO, V152, P109, DOI 10.1098/rspb.1960.0027; SHAW TI, 1959, PROC R SOC SER B-BIO, V150, P356, DOI 10.1098/rspb.1959.0027; Stewart J, 2004, SYNTHETIC COMMUN, V34, P547, DOI 10.1081/SCC-120027296; Sun J, 2005, J NAT PROD, V68, P915, DOI 10.1021/np050096g; Suzuki M, 2009, PHYTOCHEMISTRY, V70, P1410, DOI 10.1016/j.phytochem.2009.07.038; THEILER R, 1978, SCIENCE, V202, P1094, DOI 10.1126/science.202.4372.1094; Topcu G, 2003, J NAT PROD, V66, P1505, DOI 10.1021/np030176p; Verhaeghe EF, 2008, J BIOL INORG CHEM, V13, P257, DOI 10.1007/s00775-007-0319-6; VILTER H, 1995, MET IONS BIOL SYST, V31, P325; vonsHofsten A., 1978, J ULTRASTRUCT RES, V63, P108; Weinberger F, 2005, J EXP BOT, V56, P1317, DOI 10.1093/jxb/eri132; Weinberger F, 2007, J EXP BOT, V58, P4365, DOI 10.1093/jxb/erm303; WESTLUND P, 1981, BOT MAR, V24, P153, DOI 10.1515/botm.1981.24.3.153; Wever R, 2013, DALTON T, V42, P11778, DOI 10.1039/c3dt50525a; Whitfield FB, 1999, J AGR FOOD CHEM, V47, P2367, DOI 10.1021/jf981080h; WISEMAN JM, 2007, CURR SEP DRUG DEV, V22, P11, DOI DOI 10.1016/J.JASMS.2007.12.003; WOLK CP, 1968, PLANTA, V78, P371, DOI 10.1007/BF00387095; WOOLARD FX, 1976, TETRAHEDRON, V32, P2843, DOI 10.1016/0040-4020(76)80134-2; WOOLARD FX, 1979, PHYTOCHEMISTRY, V18, P617, DOI 10.1016/S0031-9422(00)84271-5; Wright AD, 2003, J NAT PROD, V66, P435, DOI 10.1021/np020274v; YOUNG DN, 1979, J PHYCOL, V15, P49, DOI 10.1111/j.0022-3646.1979.00049.x; YOUNG DN, 1980, J PHYCOL, V16, P182	98	9	9	1	64	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	AUG	2014	50	4					652	664		10.1111/jpy.12199			13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	AN3BO	WOS:000340460100005	26988449				2021-04-07	
J	Dittami, SM; Barbeyron, T; Boyen, C; Cambefort, J; Collet, G; Delage, L; Gobet, A; Groisillier, A; Leblanc, C; Michel, G; Scornet, D; Siegel, A; Tapia, JE; Tonon, T				Dittami, Simon M.; Barbeyron, Tristan; Boyen, Catherine; Cambefort, Jeanne; Collet, Guillaume; Delage, Ludovic; Gobet, Angelique; Groisillier, Agnes; Leblanc, Catherine; Michel, Gurvan; Scornet, Delphine; Siegel, Anne; Tapia, Javier E.; Tonon, Thierry			Genome and metabolic network of "Candidatus Phaeomarinobacter ectocarpi" Ec32, a new candidate genus of Alphaproteobacteria frequently associated with brown algae	FRONTIERS IN GENETICS			English	Article							MAXIMUM-LIKELIHOOD; MARINE MACROALGAE; EVOLUTION; SEQUENCE; INSIGHTS; CYTOKININ; AUXIN; BIOSYNTHESIS; SILICULOSUS; BACTERIA	Rhizobiales and related orders of Alphaproteobacteria comprise several genera of nodule-inducing symbiotic bacteria associated with plant roots. Here we describe the genome and the metabolic network of "Candidatus Phaeomarinobacter ectocarpi" Ec32, a member of a new candidate genus closely related to Rhizobiales and found in association with cultures of the filamentous brown algal model Ectocarpus. The "Ca. P. ectocarpi" genome encodes numerous metabolic pathways that may be relevant for this bacterium to interact with algae. Notably, it possesses a large set of glycoside hydrolases and transporters, which may serve to process and assimilate algal metabolites. It also harbors several proteins likely to be involved in the synthesis of algal hormones such as auxins and cytokinins, as well as the vitamins pyridoxine, biotin, and thiamine. As of today, "Ca. P. ectocarpi" has not been successfully cultured, and identical 16S rDNA sequences have been found exclusively associated with Ectocarpus. However, related sequences (>= 97% identity) have also been detected free-living and in a Fucus vesiculosus microbiome barcoding project, indicating that the candidate genus "Phaeomarinobacter" may comprise several species, which may colonize different niches.	[Dittami, Simon M.; Barbeyron, Tristan; Boyen, Catherine; Delage, Ludovic; Gobet, Angelique; Groisillier, Agnes; Leblanc, Catherine; Michel, Gurvan; Scornet, Delphine; Tonon, Thierry] Univ Paris 06, UMR 8227, Univ Paris 04, Stn Biol Roscoff, Roscoff, France; [Dittami, Simon M.; Barbeyron, Tristan; Boyen, Catherine; Delage, Ludovic; Gobet, Angelique; Groisillier, Agnes; Leblanc, Catherine; Michel, Gurvan; Scornet, Delphine; Tapia, Javier E.; Tonon, Thierry] CNRS, Stn Biol Roscoff, UMR 8227, F-29688 Roscoff, France; [Cambefort, Jeanne; Collet, Guillaume; Siegel, Anne] CNRS, IRISA UMR 6074, F-29688 Roscoff, France; [Cambefort, Jeanne; Collet, Guillaume; Siegel, Anne] Univ Rennes 1, IRISA UMR 6074, Rennes, France; [Cambefort, Jeanne; Collet, Guillaume; Siegel, Anne] INRIA, Ctr Rennes Bretagne Atlantique, Rennes, France; [Tapia, Javier E.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Santiago, Chile	Dittami, SM (corresponding author), Stn Biol Roscoff, UMR 8227, Pl Georges Teissier,CS 90074, F-29688 Roscoff, France.	simon.dittami@sb-roscoff.fr; tonon@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; Gobet, Angelique/B-7572-2013	Tonon, Thierry/0000-0002-1454-6018; Siegel, Anne/0000-0001-6542-1568; Gobet, Angelique/0000-0003-4204-8451; MICHEL, Gurvan/0000-0002-3009-6205			Arguelles JC, 2000, ARCH MICROBIOL, V174, P217, DOI 10.1007/s002030000192; Aziz RK, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-75; Ball SG, 2003, ANNU REV PLANT BIOL, V54, P207, DOI 10.1146/annurev.arplant.54.031902.134927; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Bogachev AV, 2005, BIOCHEMISTRY-MOSCOW+, V70, P143, DOI 10.1007/s10541-005-0093-4; Boussau B, 2004, P NATL ACAD SCI USA, V101, P9722, DOI 10.1073/pnas.0400975101; Brown PJB, 2011, J BACTERIOL, V193, P4567, DOI 10.1128/JB.05453-11; Butzin NC, 2013, APPL ENVIRON MICROB, V79, P7006, DOI 10.1128/AEM.01800-13; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Contreras L, 2008, J PHYCOL, V44, P1315, DOI 10.1111/j.1529-8817.2008.00575.x; Dittami SM, 2014, MOL ECOL, V23, P1656, DOI 10.1111/mec.12670; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; El-Showk S, 2013, DEVELOPMENT, V140, P1373, DOI 10.1242/dev.086371; Frebort I, 2011, J EXP BOT, V62, P2431, DOI 10.1093/jxb/err004; Goecke F, 2010, MAR ECOL PROG SER, V409, P267, DOI 10.3354/meps08607; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Gruber-Vodicka HR, 2011, P NATL ACAD SCI USA, V108, P12078, DOI 10.1073/pnas.1105347108; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Helliwell KE, 2011, MOL BIOL EVOL, V28, P2921, DOI 10.1093/molbev/msr124; Hollants J, 2013, FEMS MICROBIOL ECOL, V83, P1, DOI 10.1111/j.1574-6941.2012.01446.x; Huo YY, 2012, J BACTERIOL, V194, P197, DOI 10.1128/JB.06343-11; Karp PD, 2010, BRIEF BIOINFORM, V11, P40, DOI 10.1093/bib/bbp043; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Kottmann R, 2010, NUCLEIC ACIDS RES, V38, pD391, DOI 10.1093/nar/gkp918; Lai QL, 2011, INT J SYST EVOL MICR, V61, P271, DOI 10.1099/ijs.0.021899-0; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Le Bail A, 2010, PLANT PHYSIOL, V153, P128, DOI 10.1104/pp.109.149708; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lombard V, 2014, NUCLEIC ACIDS RES, V42, pD490, DOI 10.1093/nar/gkt1178; MacLean AM, 2007, PLANT PHYSIOL, V144, P615, DOI 10.1104/pp.107.101634; Martineau C, 2013, INT J SYST EVOL MICR, V63, P3777, DOI 10.1099/ijs.0.048124-0; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Nafisi M, 2007, PLANT CELL, V19, P2039, DOI 10.1105/tpc.107.051383; Overbeek R, 2005, NUCLEIC ACIDS RES, V33, P5691, DOI 10.1093/nar/gki866; Parfrey LW, 2011, P NATL ACAD SCI USA, V108, P13624, DOI 10.1073/pnas.1110633108; Parthasarathy H, 2007, PLOS BIOL, V5, P369, DOI 10.1371/journal.pbio.0050083; PEDERSEN M, 1968, NATURE, V218, P776, DOI 10.1038/218776a0; PEDERSEN M, 1973, PHYSIOL PLANTARUM, V28, P101, DOI 10.1111/j.1399-3054.1973.tb01158.x; PEDERSEN M, 1969, PHYSIOL PLANTARUM, V22, P977, DOI 10.1111/j.1399-3054.1969.tb07455.x; Pertry I, 2010, MOL PLANT MICROBE IN, V23, P1164, DOI 10.1094/MPMI-23-9-1164; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Preisig O, 1996, ARCH MICROBIOL, V165, P297, DOI 10.1007/s002030050330; Ray K, 2012, NAT REV GASTRO HEPAT, V9, P555, DOI [10.1038/nrgastro.2012.172, 10.1038/nrgastro.2012.165]; Redmond MC, 2012, P NATL ACAD SCI USA, V109, P20292, DOI 10.1073/pnas.1108756108; Reina-Bueno M, 2012, BMC MICROBIOL, V12, DOI 10.1186/1471-2180-12-207; Santelices B, 2007, P NATL ACAD SCI USA, V104, P19163, DOI 10.1073/pnas.0708963104; Schleheck D, 2011, STAND GENOMIC SCI, V5, P298, DOI 10.4056/sigs.2215005; SCHLESNER H, 1989, SYST APPL MICROBIOL, V12, P159, DOI 10.1016/S0723-2020(89)80008-6; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Smirnoff N, 1996, ANN BOT-LONDON, V78, P661, DOI 10.1006/anbo.1996.0175; STACKEBRANDT E, 1994, INT J SYST BACTERIOL, V44, P846, DOI 10.1099/00207713-44-4-846; Stamatakis A, 2006, BIOINFORMATICS, V22, P2688, DOI 10.1093/bioinformatics/btl446; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stothard P, 2005, BIOINFORMATICS, V21, P537, DOI 10.1093/bioinformatics/bti054; Sugawara S, 2009, P NATL ACAD SCI USA, V106, P5430, DOI 10.1073/pnas.0811226106; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Teeling H, 2012, SCIENCE, V336, P608, DOI 10.1126/science.1218344; Turner TR, 2013, GENOME BIOL, V14, DOI 10.1186/gb-2013-14-6-209; Wahl M, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00292; Wang Q, 2007, APPL ENVIRON MICROB, V73, P5261, DOI 10.1128/AEM.00062-07; Waterhouse AM, 2009, BIOINFORMATICS, V25, P1189, DOI 10.1093/bioinformatics/btp033; Wei N, 2013, TRENDS BIOTECHNOL, V31, P70, DOI 10.1016/j.tibtech.2012.10.009; Woodward AW, 2005, ANN BOT-LONDON, V95, P707, DOI 10.1093/aob/mci083; Zengler K, 2002, P NATL ACAD SCI USA, V99, P15681, DOI 10.1073/pnas.252630999; Zhang R, 2008, J INTEGR PLANT BIOL, V50, P1070, DOI 10.1111/j.1744-7909.2008.00729.x; Zheng ZY, 2013, NAT CHEM BIOL, V9, P244, DOI [10.1038/NCHEMBIO.1178, 10.1038/nchembio.1178]; Zobell CE, 1941, J MAR RES, V4, P42	72	17	17	1	17	FRONTIERS MEDIA SA	LAUSANNE	AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND		1664-8021		FRONT GENET	Front. Genet.	JUL 25	2014	5								241	10.3389/fgene.2014.00241			13	Genetics & Heredity	Genetics & Heredity	AX9MO	WOS:000347227000001	25120558	DOAJ Gold, Green Published			2021-04-07	
J	Yamagishi, T; Muller, DG; Kawai, H				Yamagishi, Takahiro; Mueller, Dieter G.; Kawai, Hiroshi			Comparative transcriptome analysis of Discosporangium mesarthrocarpum (Phaeophyceae), Schizocladia ischiensis (Schizocladiophyceae), and Phaeothamnion confervicola (Phaeothamniophyceae), with special reference to cell wall-related genes	JOURNAL OF PHYCOLOGY			English	Article						brown algae; cell wall polysaccharide; Discosporangium mesarthrocarpum; multicellularity; Phaeothamnion confervicola; Schizocladia ischiensis; Stramenopile	EXTRACELLULAR-MATRIX; EVOLUTION; INSIGHTS; SEQUENCE; GENOME; METABOLISM	Within the stramenopile lineage, only brown algae (Phaeophyceae) have evolved complex multicellularity, although some other members of the lineage (e.g., Schizocladia in Schizocladiophyceae; Phaeothamnion in Phaeothamniophyceae) also develop simple multicellular thalli. The development of an adherent extracellular matrix (ECM) is considered to be one of the key steps in the evolution of multicellularity, because ECM is involved in adhesion of cells to each other and in cell-cell communication essential for developmental, reproductive, and sophisticated defense systems. Because there are no unicellular organisms within brown algae, we considered that comparison of other stramenopile taxa closely related to brown algae and having multicellular thalli could yield clues to elucidate the evolution of multicellularity in brown algae. In this study, we investigated transcriptomes involved in cell wall polysaccharide metabolism of three stramenopile species, Discosporangium mesarthrocarpum, which is suggested to be one of the most basal taxa within Phaeophyceae, S.ischiensis, and P.confervicola. We employed 454-FLX high-throughput pyrosequencing to generate expressed sequence tag (EST) databases for these species, and performed comparative analyses between these databases and the genome sequence of the brown alga Ectocarpus siliculosus. Results indicate that cell wall polysaccharide metabolism pathways of D.mesarthrocarpum are similar to E.siliculosus, whereas those of S.ischiensis and P.confervicola are significantly different from E.siliculosus, suggesting that the components of the cell wall in S.ischiensis and P.confervicola are likely to be different from those of E.siliculosus.	[Yamagishi, Takahiro; Kawai, Hiroshi] Kobe Univ, Res Ctr Inland Seas, Kobe, Hyogo 6578501, Japan; [Mueller, Dieter G.] Univ Konstanz, Fac Biol, D-78434 Constance, Germany	Yamagishi, T (corresponding author), Kobe Univ, Res Ctr Inland Seas, Kobe, Hyogo 6578501, Japan.	takahiro@kobe-u.ac.jp			Japan Society for Promotion of SciencesMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [22370034, 25291087]; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [25840128] Funding Source: KAKEN	We are grateful to Dr. Eric C. Henry for his valuable comments on the manuscript. This study was supported by a scientific research grant from the Japan Society for Promotion of Sciences to H. K. (Project Numbers: 22370034, 25291087).	Andersen RA, 1997, J PHYCOL, V33, P1, DOI 10.1111/j.0022-3646.1997.00001.x; Andersen RA, 1998, J PHYCOL, V34, P286, DOI 10.1046/j.1529-8817.1998.340286.x; Arnaout MA, 2007, CURR OPIN CELL BIOL, V19, P495, DOI 10.1016/j.ceb.2007.08.002; Bailey JC, 1998, PROTIST, V149, P245, DOI 10.1016/S1434-4610(98)70032-X; Brownlee C, 2002, CURR OPIN PLANT BIOL, V5, P396, DOI 10.1016/S1369-5266(02)00286-8; Chevreux B, 2004, GENOME RES, V14, P1147, DOI 10.1101/gr.1917404; Chi Eun-Sup, 1999, Phycological Research, V47, P53, DOI 10.1046/j.1440-1835.1999.00151.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; Conesa A, 2005, BIOINFORMATICS, V21, P3674, DOI 10.1093/bioinformatics/bti610; De Smet I, 2009, NAT CELL BIOL, V11, P1166, DOI 10.1038/ncb1009-1166; Deboy RT, 2008, J BACTERIOL, V190, P5455, DOI 10.1128/JB.01701-07; Grant J, 2009, PROTIST, V160, P376, DOI 10.1016/j.protis.2009.01.001; HENRY EC, 1982, J PHYCOL, V18, P570; HENRY EC, 1982, J PHYCOL, V18, P550; Huang X., 1996, GENOMICS, V14, P18; Kawai H, 2003, PROTIST, V154, P211, DOI 10.1078/143446103322166518; Kawai H, 2007, J PHYCOL, V43, P186, DOI 10.1111/j.1529-8817.2006.00308.x; McCandless EL, 1981, BIOL SEAWEEDS, P559; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; OKELLY CJ, 1984, PROTOPLASMA, V123, P18, DOI 10.1007/BF01283178; Okuda K, 2004, CELLULOSE, V11, P365, DOI 10.1023/B:CELL.0000046407.22865.03; Painter T. J., 1983, POLYSACCHARIDES, P357; Phillips N, 2008, J PHYCOL, V44, P15, DOI 10.1111/j.1529-8817.2007.00435.x; Schmidt-Nielsen K., 1997, ANIMAL PHYSL ADAPTAT; Tamura Hiroshi, 1996, Phycological Research, V44, P63, DOI 10.1111/j.1440-1835.1996.tb00039.x; Tatewaki M, 1966, PHYCOLOGIA, V6, P62, DOI DOI 10.2216/I0031-8884-6-1-62.1; Thiele K, 2009, PLANT J, V58, P13, DOI 10.1111/j.1365-313X.2008.03760.x; Thompson JD, 1997, NUCLEIC ACIDS RES, V25, P4876, DOI 10.1093/nar/25.24.4876	30	2	2	0	14	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	JUN	2014	50	3					543	551		10.1111/jpy.12190			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	AJ3IN	WOS:000337559800012	26988326				2021-04-07	
J	Greco, M; Saez, CA; Brown, MT; Bitonti, MB				Greco, Maria; Saez, Claudio A.; Brown, Murray T.; Bitonti, Maria Beatrice			A Simple and Effective Method for High Quality Co-Extraction of Genomic DNA and Total RNA from Low Biomass Ectocarpus siliculosus, the Model Brown Alga	PLOS ONE			English	Article							MARINE FOULING ALGA; PHENOLIC-COMPOUNDS; COPPER TOLERANCE; LARGE-SCALE; EXTRACTION; POLYSACCHARIDES; PHAEOPHYCEAE; PROTOCOL; NORMALIZATION; LAMINARIALES	The brown seaweed Ectocarpus siliculosus is an emerging model species distributed worldwide in temperate coastal ecosystems. Over 1500 strains of E. siliculosus are available in culture from a broad range of geographic locations and ecological niches. To elucidate the molecular mechanisms underlying its capacity to cope with different environmental and biotic stressors, genomic and transcriptomic studies are necessary; this requires the co-isolation of genomic DNA and total RNA. In brown algae, extraction of nucleic acids is hindered by high concentrations of secondary metabolites that co-precipitate with nucleic acids. Here, we propose a reliable, rapid and cost-effective procedure for the co-isolation of high-quality nucleic acids using small quantities of biomass (25-, 50- and 100 mg) from strains of E. siliculosus (RHO12; LIA4A; EC524 and REP10-11) isolated from sites with different environmental conditions. The procedure employs a high pH extraction buffer (pH 9.5) which contains 100 mM Tris-HCl and 150 mM NaCl, with the addition of 5 mM DTT and 1% sarkosyl to ensure maximum solubility of nucleic acids, effective inhibition of nuclease activity and removal of interfering contaminants (e. g. polysaccharides, polyphenols). The use of sodium acetate together with isopropanol shortened precipitation time and enhanced the yields of DNA/RNA. A phenol: chlorophorm:isoamyl alcohol step was subsequently used to purify the nucleic acids. The present protocol produces high yields of nucleic acids from only 25 mg of fresh algal biomass (0.195 and 0.284 mu g mg(-1) fresh weigh of RNA and DNA, respectively) and the high quality of the extracted nucleic acids was confirmed through spectrophotometric and electrophoretic analyses. The isolated RNA can be used directly in downstream applications such as RT-PCR and the genomic DNA was suitable for PCR, producing reliable restriction enzyme digestion patterns. Co-isolation of DNA/RNA from different strains indicates that this method is likely to have wider applications for intra-and inter-specific studies on other brown algae.	[Greco, Maria; Bitonti, Maria Beatrice] Univ Calabria, Dept Biol Ecol & Earth Sci, Lab Plant Cytophysiol, I-87036 Cosenza, Italy; [Saez, Claudio A.; Brown, Murray T.] Univ Plymouth, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England; [Saez, Claudio A.] Univ Santiago Chile, Fac Quim & Biol, Dept Biol, Santiago, Chile	Greco, M (corresponding author), Univ Calabria, Dept Biol Ecol & Earth Sci, Lab Plant Cytophysiol, I-87036 Cosenza, Italy.	maria.greco@unical.it	Saez, Claudio/F-5978-2015; Bitonti, Maria/AAO-7345-2020; Brown, Murray/K-5291-2014	Saez, Claudio/0000-0002-5037-3484; Brown, Murray/0000-0003-2655-8611	CONICYT Becas Chile Scholarship [72110557]	This study was funded by CONICYT Becas Chile Scholarship (72110557) awarded to Claudio A. Saez for doctoral studies. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Ainsworth C., 1994, Plant Molecular Biology Reporter, V12, P198, DOI 10.1007/BF02668741; ARAKI S, 1992, NIPPON SUISAN GAKK, V58, P477; Asif MH, 2000, PLANT MOL BIOL REP, V18, P109, DOI 10.1007/BF02824018; Azevedo H, 2003, PLANT MOL BIOL REP, V21, P333, DOI 10.1007/BF02772582; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Birtic S, 2006, PHYTOCHEM ANALYSIS, V17, P144, DOI 10.1002/pca.903; Boehringer Mannheim, 1990, REAG MOL BIOL CAT, P255; Box MS, 2011, PLANT METHODS, V7, DOI 10.1186/1746-4811-7-7; Brooks G, 1998, BIOTECHNOLOGY HEALTH; Buckingham L., 2007, MOL DIAGNOSTICS FUND; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P365, DOI 10.1101/pdb.prot067967; COYER JA, 1995, J PHYCOL, V31, P177, DOI 10.1111/j.0022-3646.1995.00177.x; CRONSHAW J, 1958, BIOCHIM BIOPHYS ACTA, V27, P89, DOI 10.1016/0006-3002(58)90295-6; Cseke L, 2004, HDB MOL CELLULAR MET; Czechowski T, 2005, PLANT PHYSIOL, V139, P5, DOI 10.1104/pp.105.063743; Davis RH, 2004, NAT REV GENET, V5, p69A, DOI 10.1038/nrg1250; Davis TA, 2003, WATER RES, V37, P4311, DOI 10.1016/S0043-1354(03)00293-8; Deville C, 2004, J SCI FOOD AGR, V84, P1030, DOI 10.1002/jsfa.1754; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dolferus R, 1991, LAB GUIDE CELLULAR M, P133; Doyle K, 1996, SOURCE DISCOVERY PRO; Eggermont K, 1996, PLANT MOL BIOL REP, V14, P273, DOI 10.1007/BF02671663; FANG G, 1992, BIOTECHNIQUES, V13, P52; Gao JianWei, 2001, Plant Molecular Biology Reporter, V19, P185, DOI 10.1007/BF02772163; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; HALL A, 1980, NEW PHYTOL, V85, P73, DOI 10.1111/j.1469-8137.1980.tb04449.x; Hoarau G, 2007, MOL ECOL NOTES, V7, P191, DOI 10.1111/j.1471-8286.2006.01587.x; Holdt SL, 2011, J APPL PHYCOL, V23, P543, DOI 10.1007/s10811-010-9632-5; HONG YK, 1995, J APPL PHYCOL, V7, P101, DOI 10.1007/BF00693055; Hughes D., 1988, PLANT MOL BIOL REP, V6, P253, DOI DOI 10.1007/BF02670385; Jaakola L, 2001, PLANT MOL BIOL REP, V19, P125, DOI 10.1007/BF02772154; Jin HJ, 1997, J APPL PHYCOL, V9, P383, DOI 10.1023/A:1007925202219; KENDALL TL, 1991, ANAL BIOCHEM, V195, P74, DOI 10.1016/0003-2697(91)90297-7; KIRBY KS, 1956, BIOCHEM J, V64, P405, DOI 10.1042/bj0640405; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Kojima K., 2002, United State patent, Patent No. [20020192667, 2002/ 0192667 A1]; LaClaire JW, 1997, PLANT MOL BIOL REP, V15, P263, DOI 10.1023/A:1007486419576; LAIRD PW, 1991, NUCLEIC ACIDS RES, V19, P4293, DOI 10.1093/nar/19.15.4293; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Li ZW, 2005, BIOTECHNIQUES, V38, P872, DOI 10.2144/05386BM05; Liu JJ, 1998, PLANT MOL BIOL REP, V16, P87, DOI 10.1023/A:1007492421119; LOGEMANN J, 1987, ANAL BIOCHEM, V163, P16, DOI 10.1016/0003-2697(87)90086-8; MABEAU S, 1990, PHYTOCHEMISTRY, V29, P2441, DOI 10.1016/0031-9422(90)85163-A; Manickavelu A, 2007, COLLOID SURFACE B, V54, P254, DOI 10.1016/j.colsurfb.2006.10.024; MANNING K, 1991, ANAL BIOCHEM, V195, P45, DOI 10.1016/0003-2697(91)90292-2; Matthews CK, 2000, BIOCHEMISTRY-US, p[48, 209]; MAYES C, 1992, J PHYCOL, V28, P712, DOI 10.1111/j.0022-3646.1992.00712.x; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; MOREL FMM, 1979, J PHYCOL, V15, P135, DOI 10.1111/j.0022-3646.1979.00135.x; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; MURRAY MG, 1980, NUCLEIC ACIDS RES, V8, P4321, DOI 10.1093/nar/8.19.4321; Pearson G, 2006, EUR J PHYCOL, V41, P97, DOI 10.1080/09670260500505011; Peng J, 2011, MAR DRUGS, V9, P1806, DOI 10.3390/md9101806; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Phillips Naomi, 2001, Phycological Research, V49, P97, DOI 10.1111/j.1440-1835.2001.tb00239.x; Provasoli L, 1974, ALGAL PHYSL BIOCH, P741; QUATRANO RS, 1976, PLANT PHYSIOL, V58, P224, DOI 10.1104/pp.58.2.224; Rioux LE, 2007, CARBOHYD POLYM, V69, P530, DOI 10.1016/j.carbpol.2007.01.009; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Saez CA, 2012, CHEM ECOL, V28, P1, DOI 10.1080/02757540.2011.619529; Salzman RA, 1999, PLANT MOL BIOL REP, V17, P11, DOI 10.1023/A:1007520314478; Sambrook J., 2001, MOL CLONING LAB MANU, V4th; Schoenwaelder MEA, 2000, PLANT BIOLOGY, V2, P24, DOI 10.1055/s-2000-9178; Schultz D. J., 1994, Plant Molecular Biology Reporter, V12, P310, DOI 10.1007/BF02669273; Singh G., 2003, PLANT MOL BIOL REP, V21, P93, DOI DOI 10.1007/BF02773401; Smith SDA, 1996, MAR POLLUT BULL, V33, P309, DOI 10.1016/S0025-326X(96)00124-5; Smith SDA, 1996, AUST J ECOL, V21, P144, DOI 10.1111/j.1442-9993.1996.tb00595.x; Stone B. A., 1992, CHEM BIOL 1 3 BETA G; SU X, 1988, ANAL BIOCHEM, V174, P650, DOI 10.1016/0003-2697(88)90068-1; THOMAS DN, 1991, BOT ACTA, V104, P26, DOI 10.1111/j.1438-8677.1991.tb00190.x; THOMAS DN, 1991, PHYSIOL PLANTARUM, V83, P281, DOI 10.1034/j.1399-3054.1991.830212.x; Vicient CM, 1999, ANAL BIOCHEM, V268, P412, DOI 10.1006/abio.1998.3045; Villegas MJ, 2008, HELGOLAND MAR RES, V62, pS33, DOI 10.1007/s10152-007-0096-1; Vreeland V, 1998, J PHYCOL, V34, P1, DOI 10.1046/j.1529-8817.1998.340001.x; WALLACE DM, 1987, METHOD ENZYMOL, V152, P33; Wang GG, 2005, J APPL PHYCOL, V17, P75, DOI 10.1007/s10811-005-5557-9; Wang TY, 2011, GENET MOL RES, V10, P519, DOI 10.4238/vol10-1gmr1055; Wilkins Thea A., 1996, P21; Wink M, 2006, METHODS APPL MODERN; Yao JT, 2009, J APPL PHYCOL, V21, P233, DOI 10.1007/s10811-008-9354-0	86	24	25	0	57	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	MAY 27	2014	9	5							e96470	10.1371/journal.pone.0096470			13	Multidisciplinary Sciences	Science & Technology - Other Topics	AI5NG	WOS:000336914100005	24867404	DOAJ Gold, Green Published			2021-04-07	
J	Sordet, C; Contreras-Porcia, L; Lovazzano, C; Goulitquer, S; Andrade, S; Potin, P; Correa, JA				Sordet, C.; Contreras-Porcia, L.; Lovazzano, C.; Goulitquer, S.; Andrade, S.; Potin, P.; Correa, J. A.			Physiological plasticity of Dictyota kunthii (Phaeophyceae) to copper excess	AQUATIC TOXICOLOGY			English	Article						Seaweed; Copper stress; Cu complexing compounds; Exudates; Copper accumulation	SCYTOSIPHON-LOMENTARIA PHAEOPHYCEAE; LESSONIA-NIGRESCENS PHAEOPHYCEAE; INDUCED OXIDATIVE STRESS; DIFFERENT MARINE-ALGAE; NORTHERN CHILE; ECTOCARPUS-SILICULOSUS; HEAVY-METALS; FOULING ALGA; ACCUMULATION; MACROALGAE	The brown alga Dictyota kunthii is one of the dominant species in the coastal areas of northern Chile affected by copper enrichment due to accumulated mining wastes. To assess its physiological plasticity in handling copper-mediated oxidative stress, 4-days copper exposure (ca. 100 mu g/L) experiments were conducted with individuals from a copper impacted area and compared with the responses of plants from a non-impacted site. Several biochemical parameters were then evaluated and compared between populations. Results showed that individuals from the copper-impacted population normally displayed higher levels of copper content and antioxidant enzymes activity (catalase (CAT), ascorbate peroxidase (AP), dehydroascorbate reductase (DHAR), glutathione peroxidase (GP) and peroxiredoxins (PRX)). After copper exposure, antioxidant enzyme activity increased significantly in plants from the two selected sites. In addition, we found that copper-mediated oxidative stress was associated with a reduction of glutathione reductase (GR) activity. Moreover, metabolic profiling of extracellular metabolites from both populations showed a significant change after plants were exposed to copper excess in comparison with controls, strongly suggesting a copper-induced release of metabolites. The copper-binding capacity of those exudates was determined by anodic stripping voltammetry (ASV) and revealed an increased ligand capacity of the medium with plants exposed to copper excess. Results indicated that D. kunthii, regardless their origin, counteracts copper excess by various mechanisms, including metal accumulation, activation of CAT, AP, DHAR, GP and PRX, and an induced release of Cu binding compounds. Thus, plasticity in copper tolerance in D. kunthii seems constitutive, and the occurrence of a copper-tolerant ecotype seems unlikely. (C) 2014 Elsevier B.V. All rights reserved.	[Sordet, C.; Andrade, S.; Correa, J. A.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Santiago, Chile; [Contreras-Porcia, L.; Lovazzano, C.] Univ Andres Bello, Dept Ecol & Biodiversidad, Fac Ecol & Recursos Nat, Santiago, Chile; [Goulitquer, S.] CNRS, Plate Forme MetaboMER, F-29688 Roscoff, France; [Goulitquer, S.] UPMC, Biol Stn, FR2424, F-29688 Roscoff, France; [Potin, P.] Univ Paris 06, UMR CNRS 7139, Biol Stn, F-29688 Roscoff, France	Sordet, C (corresponding author), Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Alameda 340, Santiago, Chile.	camille.sordet@yahoo.fr	Contreras-Porcia, Loretto/F-9593-2016	Contreras-Porcia, Loretto/0000-0002-6511-2244; Andrade, Santiago/0000-0001-9632-6088	FONDECYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [3110034, 1120117]; FONDAP (CONICYT)Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDAP [1501-0001]; program 7; IDEALG [ANR-10-BTBR-04-02, 04-04]; European Regional Development Fund (ERDF)-FEDER, Region Bretagne [33765]	This work was supported by funding from research grants FONDECYT 3110034 to CS, FONDAP 1501-0001 (CONICYT) to the Center for Advanced Studies in Ecology 82 Biodiversity (CASEB), program 7 to JC, FONDECYT 1120117 to LC, IDEALG Grants ANR-10-BTBR-04-02 and 04-04 "Investissements d'avenir, Biotechnologies-Bioressources" and the European Regional Development Fund (ERDF)-FEDER 33765, Region Bretagne.	Andrade S, 2006, ENVIRON SCI TECHNOL, V40, P4382, DOI 10.1021/es060278c; Andrade S, 2010, CHEMOSPHERE, V78, P397, DOI 10.1016/j.chemosphere.2009.11.006; Aristilde L, 2012, ENVIRON SCI TECHNOL, V46, P5438, DOI 10.1021/es300335u; Asada K, 1999, ANNU REV PLANT PHYS, V50, P601, DOI 10.1146/annurev.arplant.50.1.601; AUST S D, 1985, Journal of Free Radicals in Biology and Medicine, V1, P3, DOI 10.1016/0748-5514(85)90025-X; Brown MT, 2012, ECOTOXICOLOGY, V21, P591, DOI 10.1007/s10646-011-0819-6; BRYAN GW, 1992, ENVIRON POLLUT, V76, P89, DOI 10.1016/0269-7491(92)90099-V; CASTILLA JC, 1978, MAR POLLUT BULL, V9, P67, DOI 10.1016/0025-326X(78)90451-4; Castilla JC, 1996, ENVIRON MONIT ASSESS, V40, P171, DOI 10.1007/BF00414390; Collen J, 1999, J PHYCOL, V35, P62, DOI 10.1046/j.1529-8817.1999.3510062.x; Contreras L, 2005, J PHYCOL, V41, P1184, DOI 10.1111/j.1529-8817.2005.00151.x; Contreras L, 2007, J PHYCOL, V43, P1320, DOI 10.1111/j.1529-8817.2007.00413.x; Contreras L, 2009, AQUAT TOXICOL, V94, P94, DOI 10.1016/j.aquatox.2009.06.004; Correa JA, 1996, ENVIRON MONIT ASSESS, V40, P41, DOI 10.1007/BF00395166; Correa JA, 1999, J APPL PHYCOL, V11, P57, DOI 10.1023/A:1008027610826; Correa JA, 2006, J EXP MAR BIOL ECOL, V335, P13, DOI 10.1016/j.jembe.2006.02.010; Cuypers A, 1999, FREE RADICAL RES, V31, pS39; Foyer C.H., 1994, CAUSES PHOTOOXIDATIV, P343; Gallego SM, 1996, PLANT SCI, V121, P151, DOI 10.1016/S0168-9452(96)04528-1; Gledhill M, 1997, J PHYCOL, V33, P2, DOI 10.1111/j.0022-3646.1997.00002.x; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; Goldstein S, 1986, FREE RADIC BIOL MED, V2, P3, DOI DOI 10.1016/0748-5514(86)90117-0; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; Hall JL, 2002, J EXP BOT, V53, P1, DOI 10.1093/jexbot/53.366.1; HALLIWELL B, 1984, BIOCHEM J, V219, P1, DOI 10.1042/bj2190001; Kim KH, 2010, BIOTECHNOL LETT, V32, P571, DOI 10.1007/s10529-009-0185-0; Lovazzano C, 2013, PHYSIOL PLANTARUM, V149, P378, DOI 10.1111/ppl.12047; Ma JF, 1997, NATURE, V390, P569, DOI 10.1038/37518; Medina M, 2005, MAR POLLUT BULL, V50, P396, DOI 10.1016/j.marpolbul.2004.11.022; Mowla SB, 2002, PLANTA, V215, P716, DOI 10.1007/s00425-002-0819-0; Noctor G, 1998, ANNU REV PLANT PHYS, V49, P249, DOI 10.1146/annurev.arplant.49.1.249; Pinto E, 2003, J PHYCOL, V39, P1008, DOI 10.1111/j.0022-3646.2003.02-193.x; Ratkevicius N, 2003, PLANT CELL ENVIRON, V26, P1599, DOI 10.1046/j.1365-3040.2003.01073.x; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Sheoran V, 2011, CRIT REV ENV SCI TEC, V41, P168, DOI 10.1080/10643380902718418; Stauber JL, 2005, MAR POLLUT BULL, V50, P1363, DOI 10.1016/j.marpolbul.2005.05.008; SUEUR S, 1982, LIMNOL OCEANOGR, V27, P536, DOI 10.4319/lo.1982.27.3.0536; Sunda WG, 1998, SCI TOTAL ENVIRON, V219, P165, DOI 10.1016/S0048-9697(98)00226-5; SUNDA WG, 1992, LIMNOL OCEANOGR, V37, P25, DOI 10.4319/lo.1992.37.1.0025; Vasconcelos MTSD, 2008, MAR ENVIRON RES, V66, P499, DOI 10.1016/j.marenvres.2008.07.002; Vasconcelos MTSD, 2002, MAR CHEM, V77, P187, DOI 10.1016/S0304-4203(01)00087-1; Vasconcelos MTSD, 2001, MAR CHEM, V74, P65, DOI 10.1016/S0304-4203(00)00096-7; Weckx JEJ, 1996, PHYSIOL PLANTARUM, V96, P506, DOI 10.1111/j.1399-3054.1996.tb00465.x; Wu TM, 2009, AQUAT TOXICOL, V94, P275, DOI 10.1016/j.aquatox.2009.07.010; Wu TM, 2008, PHYCOLOGIA, V47, P346, DOI 10.2216/PH07-77.1; Zar J.H., 2010, BIOSTATISTICAL ANAL	46	8	8	0	42	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	0166-445X	1879-1514		AQUAT TOXICOL	Aquat. Toxicol.	MAY	2014	150						220	228		10.1016/j.aquatox.2014.02.018			9	Marine & Freshwater Biology; Toxicology	Marine & Freshwater Biology; Toxicology	AH5RF	WOS:000336187900025	24704518	Green Published			2021-04-07	
J	Ritter, A; Dittami, SM; Goulitquer, S; Correa, JA; Boyen, C; Potin, P; Tonon, T				Ritter, Andres; Dittami, Simon M.; Goulitquer, Sophie; Correa, Juan A.; Boyen, Catherine; Potin, Philippe; Tonon, Thierry			Transcriptomic and metabolomic analysis of copper stress acclimation in Ectocarpus siliculosus highlights signaling and tolerance mechanisms in brown algae	BMC PLANT BIOLOGY			English	Article						Brown algae; Heavy metal; Copper stress response; Primary metabolism; ABC transporters; Oxylipins	SPECTROMETRY DATA; GENE ONTOLOGY; MARINE-ALGAE; FATTY-ACIDS; CELL-DEATH; RESPONSES; DETOXIFICATION; PROTEINS; NORMALIZATION; ARABIDOPSIS	Background: Brown algae are sessile macro-organisms of great ecological relevance in coastal ecosystems. They evolved independently from land plants and other multicellular lineages, and therefore hold several original ontogenic and metabolic features. Most brown algae grow along the coastal zone where they face frequent environmental changes, including exposure to toxic levels of heavy metals such as copper (Cu). Results: We carried out large-scale transcriptomic and metabolomic analyses to decipher the short-term acclimation of the brown algal model E. siliculosus to Cu stress, and compared these data to results known for other abiotic stressors. This comparison demonstrates that Cu induces oxidative stress in E. siliculosus as illustrated by the transcriptomic overlap between Cu and H2O2 treatments. The common response to Cu and H2O2 consisted in the activation of the oxylipin and the repression of inositol signaling pathways, together with the regulation of genes coding for several transcription-associated proteins. Concomitantly, Cu stress specifically activated a set of genes coding for orthologs of ABC transporters, a P1B-type ATPase, ROS detoxification systems such as a vanadium-dependent bromoperoxidase, and induced an increase of free fatty acid contents. Finally we observed, as a common abiotic stress mechanism, the activation of autophagic processes on one hand and the repression of genes involved in nitrogen assimilation on the other hand. Conclusions: Comparisons with data from green plants indicate that some processes involved in Cu and oxidative stress response are conserved across these two distant lineages. At the same time the high number of yet uncharacterized brown alga-specific genes induced in response to copper stress underlines the potential to discover new components and molecular interactions unique to these organisms. Of particular interest for future research is the potential cross-talk between reactive oxygen species (ROS)-, myo-inositol-, and oxylipin signaling.	[Ritter, Andres; Dittami, Simon M.; Boyen, Catherine; Potin, Philippe; Tonon, Thierry] Univ Paris 06, UMR 8227, Stn Biol Roscoff, Univ Sorbonne, F-29688 Roscoff, France; [Ritter, Andres; Dittami, Simon M.; Boyen, Catherine; Potin, Philippe; Tonon, Thierry] CNRS, UMR 8227, Stn Biol Roscoff, F-29688 Roscoff, France; [Ritter, Andres; Correa, Juan A.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Appl Ecol & Sustainabil, Dept Ecol, Santiago, Chile; [Goulitquer, Sophie] CNRS, Plate Forme MetaboMER, F-29680 Roscoff, France; [Goulitquer, Sophie] UPMC, Biol Stn, FR2424, F-29680 Roscoff, France	Tonon, T (corresponding author), Univ Paris 06, UMR 8227, Stn Biol Roscoff, Univ Sorbonne, CS 90074, F-29688 Roscoff, France.	tonon@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; Dittami, Simon/E-8354-2011	Tonon, Thierry/0000-0002-1454-6018; Ritter, Andres/0000-0001-7011-6824; Dittami, Simon/0000-0001-7987-7523	Marine Genomics Europe NoE 7 (EU) [GOCE-CT-2004-505403]; French Embassy; CONICYT of Chile through PhD fellowship; Laboratoire International Associe 'Dispersal and Adaptation of Marine Species' (LIA DIAMS) PUC, Chile; CNRS-UPMC, France; French Government via the National Research Agency through the investment expenditure program IDEALG [ANR-10-BTBR-04]; FONDAP [1501-0001 (Program 7)]	We are especially grateful to Laurence Dartevelle for valuable help with Ectocarpus siliculosus cultivation. This work has been partially funded by Marine Genomics Europe NoE 7 (EU contract no GOCE-CT-2004-505403), and the French Embassy and the CONICYT of Chile through PhD fellowship to A. R. This work was also supported by the Laboratoire International Associe 'Dispersal and Adaptation of Marine Species' (LIA DIAMS) PUC, Chile, and CNRS-UPMC, France. S. G. benefited from the support of the French Government via the National Research Agency through the investment expenditure program IDEALG (ANR-10-BTBR-04). Additional support came from FONDAP 1501-0001 (Program 7) to J.A.C. and A. R.	Abascal F, 2005, BIOINFORMATICS, V21, P2104, DOI 10.1093/bioinformatics/bti263; Andres-Colas N, 2006, PLANT J, V45, P225, DOI 10.1111/j.1365-313X.2005.02601.x; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Arnold TM, 2001, J PHYCOL, V37, P1026, DOI 10.1046/j.1529-8817.2001.01130.x; Ashburner M, 2000, NAT GENET, V25, P25, DOI 10.1038/75556; Belknap WR, 1996, TRENDS PLANT SCI, V1, P331; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; Blaby-Haas CE, 2012, BBA-MOL CELL RES, V1823, P1531, DOI 10.1016/j.bbamcr.2012.04.010; Bolstad BM, 2003, BIOINFORMATICS, V19, P185, DOI 10.1093/bioinformatics/19.2.185; Chaouch S, 2010, NEW PHYTOL, V188, P711, DOI 10.1111/j.1469-8137.2010.03453.x; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chmielowska-Bak J, 2012, J CELL COMMUN SIGNAL, V6, P191, DOI 10.1007/s12079-012-0173-3; Cock JM, 2011, CURR BIOL, V21, pR573, DOI 10.1016/j.cub.2011.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Contreras L, 2005, J PHYCOL, V41, P1184, DOI 10.1111/j.1529-8817.2005.00151.x; Contreras L, 2010, AQUAT TOXICOL, V96, P85, DOI 10.1016/j.aquatox.2009.10.007; Correa JA, 1999, J APPL PHYCOL, V11, P57, DOI 10.1023/A:1008027610826; Dalisay DS, 2006, MICROBIOL-SGM, V152, P2875, DOI 10.1099/mic.0.29158-0; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Dean M, 2001, GENOME RES, V11, P1156, DOI 10.1101/gr.GR-1649R; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Dittami SM, 2010, BMC EVOL BIOL, V10, DOI 10.1186/1471-2148-10-365; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Donahue JL, 2010, PLANT CELL, V22, P888, DOI 10.1105/tpc.109.071779; Dring MJ, 2006, ADV BOT RES, V43, P175, DOI 10.1016/S0065-2296(05)43004-9; Eddy SR, 2011, PLOS COMPUT BIOL, V7, DOI 10.1371/journal.pcbi.1002195; Farmer EE, 2007, CURR OPIN PLANT BIOL, V10, P380, DOI 10.1016/j.pbi.2007.04.019; FERNANDES JC, 1991, BOT REV, V57, P246, DOI 10.1007/BF02858564; Foyer CH, 2009, ANNU REV PLANT BIOL, V60, P455, DOI 10.1146/annurev.arplant.043008.091948; Gillaspy GE, 2011, NEW PHYTOL, V192, P823, DOI 10.1111/j.1469-8137.2011.03939.x; Gledhill M, 1997, J PHYCOL, V33, P2, DOI 10.1111/j.0022-3646.1997.00002.x; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Hall JL, 2002, J EXP BOT, V53, P1, DOI 10.1093/jexbot/53.366.1; HALLIWELL B, 1992, FEBS LETT, V307, P108, DOI 10.1016/0014-5793(92)80911-Y; Hockin NL, 2012, PLANT PHYSIOL, V158, P299, DOI 10.1104/pp.111.184333; Irizarry RA, 2003, BIOSTATISTICS, V4, P249, DOI 10.1093/biostatistics/4.2.249; Kazan K, 2008, PLANT PHYSIOL, V146, P1459, DOI 10.1104/pp.107.115717; Kelley LA, 2009, NAT PROTOC, V4, P363, DOI 10.1038/nprot.2009.2; Kim S, 2013, P NATL ACAD SCI USA, V110, P773, DOI 10.1073/pnas.1214159110; Kupper FC, 2009, PLANT CELL PHYSIOL, V50, P789, DOI 10.1093/pcp/pcp023; Kuhl C, 2012, ANAL CHEM, V84, P283, DOI 10.1021/ac202450g; Kupper H, 2002, J PHYCOL, V38, P429, DOI 10.1046/j.1529-8817.2002.01148.x; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Livingstone DR, 2001, MAR POLLUT BULL, V42, P656, DOI 10.1016/S0025-326X(01)00060-1; Maksymiec W, 1997, PHOTOSYNTHETICA, V34, P321, DOI 10.1023/A:1006818815528; Medina M, 2005, MAR POLLUT BULL, V50, P396, DOI 10.1016/j.marpolbul.2004.11.022; Meng PH, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0007364; Miller R, 2010, PLANT PHYSIOL, V154, P1737, DOI 10.1104/pp.110.165159; Morris CA, 1999, BIOCHEM J, V338, P553, DOI 10.1042/0264-6021:3380553; Mueller S, 2008, PLANT CELL, V20, P768, DOI 10.1105/tpc.107.054809; Nielsen HD, 2010, MAR POLLUT BULL, V60, P710, DOI 10.1016/j.marpolbul.2009.11.025; Noctor G, 1998, J EXP BOT, V49, P1895, DOI 10.1093/jexbot/49.329.1895; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; Puig S, 2007, PLANT CELL ENVIRON, V30, P271, DOI 10.1111/j.1365-3040.2007.01642.x; Rayko E, 2010, NEW PHYTOL, V188, P52, DOI 10.1111/j.1469-8137.2010.03371.x; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Saeed AI, 2003, BIOTECHNIQUES, V34, P374, DOI 10.2144/03342mt01; Schauser L, 1999, NATURE, V402, P191, DOI 10.1038/46058; Smith CA, 2006, ANAL CHEM, V78, P779, DOI 10.1021/ac051437y; Stotz HU, 2013, J EXP BOT, V64, P963, DOI 10.1093/jxb/ers389; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; Tonon T, 2011, OMICS, V15, P883, DOI 10.1089/omi.2011.0089; Verrier PJ, 2008, TRENDS PLANT SCI, V13, P151, DOI 10.1016/j.tplants.2008.02.001; Vinayagam A, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-161; Wanke D, 2010, PLANT BIOLOGY, V12, P15, DOI 10.1111/j.1438-8677.2010.00380.x; Wenzel JJ, 2007, FRONT BIOSCI, V12, P16; Wu CH, 2006, NUCLEIC ACIDS RES, V34, pD187, DOI 10.1093/nar/gkj161; Yokosho K, 2011, PLANT J, V68, P1061, DOI 10.1111/j.1365-313X.2011.04757.x; Yruela I, 1996, J BIOL CHEM, V271, P27408, DOI 10.1074/jbc.271.44.27408	73	62	63	2	113	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2229			BMC PLANT BIOL	BMC Plant Biol.	MAY 1	2014	14								116	10.1186/1471-2229-14-116			17	Plant Sciences	Plant Sciences	AH0EB	WOS:000335790800001	24885189	DOAJ Gold, Green Published			2021-04-07	
J	Perez, M; Garcia, M; Sanchez, M; Stupak, M; Mazzuca, M; Palermo, JA; Blustein, G				Perez, Miriam; Garcia, Monica; Sanchez, Marianela; Stupak, Mirta; Mazzuca, Marcia; Palermo, Jorge A.; Blustein, Guillermo			Effect of secochiliolide acid isolated from the Patagonian shrub Nardophyllum bryoides as active component in antifouling paints	INTERNATIONAL BIODETERIORATION & BIODEGRADATION			English	Article						Secochiliolide acid; Terpenoids; Nardophyllum bryoides; Soluble matrix antifouling paints	NATURAL-PRODUCT ANTIFOULANTS; BLUE MUSSEL; SECONDARY METABOLITES; LARVAL SETTLEMENT; DITERPENES; DERIVATIVES; EXTRACTS; ANALOGS; PLANTS; REPELLENTS	Environmental concerns about the use of toxic antifoulants have led to an increased interest in the development of new alternatives. So far, most of the antifouling natural products have been obtained from marine organisms. However, some secondary metabolites from terrestrial plants could be promising antifoulant candidates. The antifouling performance of secochiliolide acid, the main component isolated from Nardophyllum bryoides ethanolic extract, was evaluated for inclusion in rosin-based coatings. Field testing was conducted during the summer months at Mar del Plata harbor, Argentina. The results indicated that secochiliolide acid-based paints completely inhibited the settlement of Bugula neritina colonies, Polydora sp., Hydroides elegans, Corophium sp. and solitary ascidians, and also reduced the attachment of some algae as Enteromorpha intestinalis and Ectocarpus sp. In addition, a lower density and diversity of microfouling species was registered. These results highlighted the importance of terrestrial plants as a sustainable source of potential environmentally friendly antifoulants. (C) 2014 Elsevier Ltd. All rights reserved.	[Perez, Miriam; Garcia, Monica; Stupak, Mirta; Blustein, Guillermo] Ctr Invest & Desarrollo Tecnol Pinturas CIDEPINT, RA-1900 La Plata, Buenos Aires, Argentina; [Perez, Miriam] Univ Nacl La Plata, Fac Ciencias Nat & Museo, La Plata, Buenos Aires, Argentina; [Sanchez, Marianela; Palermo, Jorge A.] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, UMYMFOR, RA-1428 Buenos Aires, DF, Argentina; [Mazzuca, Marcia] Univ Nacl Patagonia San Juan Bosco, Fac Ciencias Nat, Dpto Quim, RA-9000 Comodoro Rivadavia, Chubut, Argentina; [Blustein, Guillermo] Univ Nacl La Plata, Fac Ciencias Agr & Forestales, La Plata, Buenos Aires, Argentina	Blustein, G (corresponding author), Ctr Invest & Desarrollo Tecnol Pinturas CIDEPINT, Av 52 E 121 & 122, RA-1900 La Plata, Buenos Aires, Argentina.	antifouling@cidepint.gov.ar		Sanchez, Marianela/0000-0001-8008-1856; Palermo, Jorge/0000-0002-0892-9389	ANPCYTANPCyT [PICT 2010-1808]; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); Comision de Investigaciones Cientificas de la provincia de Buenos Aires (CIC); Universidad Nacional de La PlataNational University of La Plata	This research was supported by a Grant from ANPCYT (PICT 2010-1808), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Comision de Investigaciones Cientificas de la provincia de Buenos Aires (CIC) and Universidad Nacional de La Plata.	Abdelgaleil SAM, 2001, TETRAHEDRON, V57, P119, DOI 10.1016/S0040-4020(00)00994-7; Alam MS, 2011, J KOREAN SOC APPL BI, V54, P725, DOI 10.3839/jksabc.2011.109; Angarano MB, 2007, BIOFOULING, V23, P295, DOI 10.1080/08927010701371439; Barboza Gloria E., 2009, Kurtziana, V34, P7; Blihoghe D, 2011, BIOFOULING, V27, P99, DOI 10.1080/08927014.2010.542587; Blustein G, 2009, BIOFOULING, V25, P573, DOI 10.1080/08927010902995572; Bonifacino JM, 2005, TAXON, V54, P688, DOI 10.2307/25065425; Brango Vanegas J., 2011, THESIS U NACL COLOMB; Casero C, 2013, PHYTOMEDICINE, V20, P133, DOI 10.1016/j.phymed.2012.11.003; Chiruvella Kishore K, 2007, Int J Biomed Sci, V3, P269; Clare AS, 1998, J MAR BIOTECHNOL, V6, P3; Clare AS, 1999, MAR BIOTECHNOL, V1, P427, DOI 10.1007/PL00011799; Clare AS, 1996, BIOFOULING, V9, P211, DOI 10.1080/08927019609378304; D'Almeida R., 2007, B LATINOAM CARIBE PL, V5, P242; D'Almeida Romina E., 2011, Lilloa, V48, P17; Da Gama BAP, 2002, BIOFOULING, V18, P13, DOI 10.1080/08927010290017680; De Chen J, 2008, MOLECULES, V13, P212, DOI 10.3390/molecules13020212; de Nys R, 2006, MAR MOL BIOTECHNOL, V42, P55; DellaGreca M, 2001, PHYTOCHEMISTRY, V58, P299, DOI 10.1016/S0031-9422(01)00203-5; Dobretsov S, 2007, FEMS MICROBIOL ECOL, V60, P177, DOI 10.1111/j.1574-6941.2007.00285.x; Etoh H, 2002, BIOSCI BIOTECH BIOCH, V66, P1748, DOI 10.1271/bbb.66.1748; Evans SM, 1999, BIOFOULING, V14, P117, DOI 10.1080/08927019909378403; Faimali M, 2003, BIOFOULING, V19, P213, DOI 10.1080/0892701021000044228; Feng DQ, 2009, BIOFOULING, V25, P181, DOI 10.1080/08927010802669210; Floerl O, 2005, BIOL INVASIONS, V7, P459, DOI 10.1007/s10530-004-4863-5; Floerl O, 2004, ECOL APPL, V14, P1724, DOI 10.1890/03-5399; FOSTER MS, 1991, J EXP MAR BIOL ECOL, V146, P193, DOI 10.1016/0022-0981(91)90025-R; Gavin NM, 2011, AQUAT BOT, V95, P242, DOI 10.1016/j.aquabot.2011.07.004; Goransson U, 2004, J NAT PROD, V67, P1287, DOI 10.1021/np0499719; HARADA A, 1985, AGR BIOL CHEM TOKYO, V49, P1887, DOI 10.1080/00021369.1985.10866999; Hellio C, 2000, APPL MICROBIOL BIOT, V54, P543, DOI 10.1007/s002530000413; Hellio C, 2005, MAR BIOTECHNOL, V7, P297, DOI 10.1007/s10126-004-3150-x; HYODO S, 1992, BIOSCI BIOTECH BIOCH, V56, P138, DOI 10.1271/bbb.56.138; Iyapparaj P., 2012, ANAS, V6, P153; JAKUPOVIC J, 1986, TETRAHEDRON, V42, P1305, DOI 10.1016/S0040-4020(01)87350-6; Kamal M., 2011, INT J MED PHARM SCI, V1, P1; KEIFER PA, 1986, J ORG CHEM, V51, P4450, DOI 10.1021/jo00373a020; Leer-Andersen M, 2003, J MAR SCI TECHNOL, V8, P26, DOI 10.1007/s10773-003-0150-y; Lejars M, 2012, CHEM REV, V112, P4347, DOI 10.1021/cr200350v; Lewthwaite J.C., 1985, R I NAVAL ARCHITECTS, V127, P269; Li YX, 2013, MAR BIOTECHNOL, V15, P552, DOI 10.1007/s10126-013-9502-7; Lin XY, 2009, ECOL ENG, V35, P502, DOI 10.1016/j.ecoleng.2008.04.013; Lowery CA, 2009, J AM CHEM SOC, V131, P15584, DOI 10.1021/ja9066783; Marechal JP, 2009, INT J MOL SCI, V10, P4623, DOI 10.3390/ijms10114623; Minchin D, 2003, BIOFOULING, V19, P111, DOI 10.1080/0892701021000057891; Ovesen RG, 2011, ENVIRON TOXICOL CHEM, V30, P1190, DOI 10.1002/etc.496; Perez M, 2007, BIOFOULING, V23, P151, DOI 10.1080/08927010701189484; Pinori E, 2011, BIOFOULING, V27, P941, DOI 10.1080/08927014.2011.616636; Qian PY, 2012, HDB MARINE NATURAL P, P749; Raveendran TV, 2009, CURR SCI INDIA, V97, P508; Rittschof D, 2001, CRC MAR SCI, P543; Sanchez M, 2010, PHYTOCHEMISTRY, V71, P1395, DOI 10.1016/j.phytochem.2010.04.019; Sardari S., 2000, STUD NAT PROD CHEM, V23, P335; Sawant S., 1997, P EM NONM MAT MAR EN; SAWANT SS, 1994, RECENT DEVELOPMENTS IN BIOFOULING CONTROL, P275; Sawant SS, 1995, INDIAN J MAR SCI, V24, P229; Schultz MP, 2011, BIOFOULING, V27, P87, DOI 10.1080/08927014.2010.542809; Schultz MP, 2007, BIOFOULING, V23, P331, DOI 10.1080/08927010701461974; Shao CL, 2011, J NAT PROD, V74, P629, DOI 10.1021/np100641b; SHAPIRO SS, 1965, BIOMETRIKA, V52, P591, DOI 10.1093/biomet/52.3-4.591; Siless GE, 2013, BIOORG MED CHEM LETT, V23, P4964, DOI 10.1016/j.bmcl.2013.06.064; Singh IP, 1996, BIOSCI BIOTECH BIOCH, V60, P1522, DOI 10.1271/bbb.60.1522; Sjogren M, 2008, BIOFOULING, V24, P251, DOI 10.1080/08927010802072753; Steinberg PD, 1997, BIODEGRADATION, V8, P211, DOI 10.1023/A:1008236901790; Steinberg PD, 2002, J PHYCOL, V38, P621, DOI 10.1046/j.1529-8817.2002.02042.x; Stupak ME, 2003, INT BIODETER BIODEGR, V52, P49, DOI 10.1016/S0964-8305(03)00035-0; TAKASAWA R, 1990, AGR BIOL CHEM TOKYO, V54, P1607, DOI 10.1080/00021369.1990.10870144; Tan LT, 2010, BIOFOULING, V26, P685, DOI 10.1080/08927014.2010.508343; Tran TD, 2012, MOLECULES, V17, P6684, DOI 10.3390/molecules17066684; Thomas KV, 2010, BIOFOULING, V26, P73, DOI 10.1080/08927010903216564; Tsoukatou M, 2007, MOLECULES, V12, P1022, DOI 10.3390/12051022; Viju N, 2013, SAUDI J BIOL SCI, V20, P85, DOI 10.1016/j.sjbs.2012.11.002; Villa F, 2010, BIOFOULING, V26, P739, DOI 10.1080/08927014.2010.511197; Wang ZC, 2013, INT J MOL SCI, V14, P1197, DOI 10.3390/ijms14011197; Waridel P, 2003, PHYTOCHEMISTRY, V64, P1309, DOI 10.1016/j.phytochem.2003.08.014; Widelski J, 2009, MOLECULES, V14, P2729, DOI 10.3390/molecules14082729; Xu QW, 2005, ENVIRON TOXICOL, V20, P467, DOI 10.1002/tox.20134; Xu QW, 2005, ENVIRON SCI POLLUT R, V12, P278, DOI 10.1065/espr2005.04.244; Xu Y, 2010, BIORESOURCE TECHNOL, V101, P1331, DOI 10.1016/j.biortech.2009.09.046; Nguyen XC, 2013, J NAT PROD, V76, P1313, DOI 10.1021/np400288j; YAMASHITA N, 1989, AGR BIOL CHEM TOKYO, V53, P1383, DOI 10.1080/00021369.1989.10869456; Yamashita N., 1986, AGR BIOL CHEM TOKYO, V53, P2827; YOSHIOKA A, 1990, AGR BIOL CHEM TOKYO, V54, P3355, DOI 10.1080/00021369.1990.10870460; Zhou XJ, 2009, BIOFOULING, V25, P69, DOI 10.1080/08927010802455941	84	13	13	1	28	ELSEVIER SCI LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND	0964-8305	1879-0208		INT BIODETER BIODEGR	Int. Biodeterior. Biodegrad.	APR	2014	89						37	44		10.1016/j.ibiod.2014.01.009			8	Biotechnology & Applied Microbiology; Environmental Sciences	Biotechnology & Applied Microbiology; Environmental Sciences & Ecology	AE2CY	WOS:000333782100006		Green Published			2021-04-07	
J	Deng, YY; Yao, JT; Fu, G; Guo, H; Duan, DL				Deng, Yunyan; Yao, Jianting; Fu, Gang; Guo, Hui; Duan, Delin			Isolation, Expression, and Characterization of Blue Light Receptor AUREOCHROME Gene From Saccharina japonica (Laminariales, Phaeophyceae)	MARINE BIOTECHNOLOGY			English	Article						Aureochrome; Blue light receptor; bZIP domain; LOV domain; Saccharina japonica; Stramenopile	SATURATED PHOTOSYNTHESIS; TRANSCRIPTION FACTOR; CELL-DIVISION; CLUSTAL-W; PROTEIN; GENOME; REPRODUCTION; PHOTOTROPIN; EVOLUTION; PLANTS	Photosynthetic stramenopile have chloroplasts of secondary endosymbiotic origin and are significant as aquatic primary productivity and biomass production. In marine environments, many photosynthetic stramenopiles utilize blue light to regulate growth, development, and organelle movement. Aureochrome (AUREO) is a new type blue light photoreceptor specific in photosynthetic stramenopiles. Previously, several AUREO orthologs were reported in genomes of stramenopile members, but the full-length cDNA sequences were completed only in Vaucheria frigida (Xanthophyceae), Fucus distichus (Phaeophyceae), and Ochromonas danica (Chrysophyceae). In this study, the full-length cDNA of AUREO from Saccharina japonica (designated as SjAUREO) was isolated based on homologous cloning and the rapid amplification of cDNA ends (RACE). It characterized by the full length of 1,013 bp with an open reading frame of 612 bp, which encoded a polypeptide of 203 amino acids with predicted molecular weight of 23.08 kDa and theoretical isoelectric point of 7.63. The deduced amino acid sequence of SjAUREO contained one N-terminal basic region/leucine zipper (bZIP) transcription regulation domain and a single light-, oxygen-, or voltage-sensitive (LOV) domain near the C-terminus. Homologous analysis showed that SjAUREO shared 40-92 % similarities with those of other photosynthetic stramenopiles. Phylogenetic analysis revealed close phylogenetic affinity between SjAUREO and AUREO4 of brown alga Ectocarpus siliculosus. Real-time PCR detection revealed that the SjAUREO transcription was markedly increased under BL exposure and dramatically upregulated in the 1-month juvenile sporophyte than those in the 2 and 3-month materials, which indirectly reflected the SjAUREO associated with the BL-mediated photomorphogenesis during the growth and early development of juvenile sporophytes. In vitro expression showed one distinct band existed at similar to 27 kDa, and western blot detection proved that it was positive to the anti-His antibody with high specificity. Our results enriched the knowledge of AUREO properties in S. japonica and provided clues to explore the mechanisms underlying diverse physiological responses mediated by BL photoreceptors AUREO in the photosynthetic stramenopiles.	[Deng, Yunyan; Yao, Jianting; Fu, Gang; Duan, Delin] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Guo, Hui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China	Duan, DL (corresponding author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.	dlduan@qdio.ac.cn	Duan, Delin/G-9002-2011		National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [40976085]; Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project	This research was supported by National Natural Science Foundation of China (No. 40976085) and Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project. We sincerely thank Drs. Hironao Kataoka and Fumio Takahashi for their valuable comments and critical reading of the manuscript. Thanks are either due to Lin Xiao, Jin Zhao, and Ge Liu for their help with the experiments. The authors acknowledged the anonymous reviewers for the critical comments and suggestions for the manuscript.	AHMAD M, 1993, NATURE, V366, P162, DOI 10.1038/366162a0; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Banerjee R, 2005, PLANTA, V220, P498, DOI 10.1007/s00425-004-1418-z; Bisova K, 2005, PLANT PHYSIOL, V137, P475, DOI 10.1104/pp.104.054155; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Briggs WR, 2001, PLANT CELL, V13, P993, DOI 10.1105/tpc.13.5.993; Cashmore AR, 1999, SCIENCE, V284, P760, DOI 10.1126/science.284.5415.760; Cavalier-Smith TA, 1986, PROGR PHYCOLOGICAL R; Cheng P, 2003, P NATL ACAD SCI USA, V100, P5938, DOI 10.1073/pnas.1031791100; Christie JM, 2007, ANNU REV PLANT BIOL, V58, P21, DOI 10.1146/annurev.arplant.58.032806.103951; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Crosson S, 2001, P NATL ACAD SCI USA, V98, P2995, DOI 10.1073/pnas.051520298; Depauw FA, 2012, J EXP BOT, V63, P1575, DOI 10.1093/jxb/ers005; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; EDMUNDS L N JR, 1984, Chronobiology International, V1, P1, DOI 10.3109/07420528409059112; Ehlert A, 2006, PLANT J, V46, P890, DOI 10.1111/j.1365-313X.2006.02731.x; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; Gasteiger E, 2005, PROTEOMICS PROTOCOLS; Geourjon C, 1995, COMPUT APPL BIOSCI, V11, P681; Hagiwara S, 2002, PHOTOCHEM PHOTOBIOL, V76, P105, DOI 10.1562/0031-8655(2002)076<0105:CGOPOC>2.0.CO;2; Hegemann P, 2008, ANNU REV PLANT BIOL, V59, P167, DOI 10.1146/annurev.arplant.59.032607.092847; Hisatomi O, 2013, PLANT CELL PHYSIOL, V54, P93, DOI 10.1093/pcp/pcs160; Huysman MJJ, 2013, PLANT CELL, V25, P215, DOI 10.1105/tpc.112.106377; Ishikawa M, 2009, PLANTA, V230, P543, DOI 10.1007/s00425-009-0967-6; Jakoby M, 2002, TRENDS PLANT SCI, V7, P106, DOI 10.1016/S1360-1385(01)02223-3; Janouskovec J, 2010, P NATL ACAD SCI USA, V107, P10949, DOI 10.1073/pnas.1003335107; Kirk J.T.O., 1994, LIGHT PHOTOSYNTHESIS; Krogh A, 2001, J MOL BIOL, V305, P567, DOI 10.1006/jmbi.2000.4315; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; LUNING K, 1980, J PHYCOL, V16, P1; LUNING K, 1975, MAR BIOL, V29, P195, DOI 10.1007/BF00391846; LUNING K, 1972, PLANTA, V104, P252, DOI 10.1007/BF00387080; Mitre D, 2012, STRUCTURE, V20, P698, DOI 10.1016/j.str.2012.02.016; Moulager M, 2007, PLANT PHYSIOL, V144, P1360, DOI 10.1104/pp.107.096149; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; Rayko E, 2010, NEW PHYTOL, V188, P52, DOI 10.1111/j.1469-8137.2010.03371.x; Schmittgen TD, 2000, ANAL BIOCHEM, V285, P194, DOI 10.1006/abio.2000.4753; Shi Cuijuan, 2005, Chinese Journal of Oceanology and Limnology, V23, P323; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; Suetsugu N, 2013, PLANT CELL PHYSIOL, V54, P8, DOI 10.1093/pcp/pcs165; Takahashi F, 2007, P NATL ACAD SCI USA, V104, P19625, DOI 10.1073/pnas.0707692104; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/msr121, 10.1093/molbev/mst197]; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; Van der Horst MA, 2004, ACCOUNTS CHEM RES, V37, P13, DOI 10.1021/ar020219d; Wang WJ, 2010, MAR BIOL, V157, P1811, DOI 10.1007/s00227-010-1453-1; Yao JT, 2009, J APPL PHYCOL, V21, P233, DOI 10.1007/s10811-008-9354-0; Yoon HS, 2002, P NATL ACAD SCI USA, V99, P11724, DOI 10.1073/pnas.172234799	50	15	16	2	41	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	1436-2228	1436-2236		MAR BIOTECHNOL	Mar. Biotechnol.	APR	2014	16	2					135	143		10.1007/s10126-013-9539-7			9	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	AB2WG	WOS:000331652200002	24052494				2021-04-07	
J	Rockwell, NC; Duanmu, D; Martin, SS; Bachy, C; Price, DC; Bhattacharya, D; Worden, AZ; Lagarias, JC				Rockwell, Nathan C.; Duanmu, Deqiang; Martin, Shelley S.; Bachy, Charles; Price, Dana C.; Bhattacharya, Debashish; Worden, Alexandra Z.; Lagarias, J. Clark			Eukaryotic algal phytochromes span the visible spectrum	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA			English	Article						biliprotein; photoswitch; photochemistry; bilin; tetrapyrrole	CHROMOPHORE-BINDING DOMAIN; PACIFIC-OCEAN; DXCF-CYANOBACTERIOCHROMES; MESOTAENIUM-CALDARIORUM; SIGNALING MECHANISMS; EVOLUTIONARY HISTORY; COCCOID PRASINOPHYTE; CIRCULAR-DICHROISM; CRYSTAL-STRUCTURE; CPH1 PHYTOCHROME	Plant phytochromes are photoswitchable red/far-red photoreceptors that allow competition with neighboring plants for photo-synthetically active red light. In aquatic environments, red and far-red light are rapidly attenuated with depth; therefore, photosynthetic species must use shorter wavelengths of light. Nevertheless, phytochrome-related proteins are found in recently sequenced genomes of many eukaryotic algae from aquatic environments. We examined the photosensory properties of seven phytochromes from diverse algae: four prasinophyte (green algal) species, the heterokont (brown algal) Ectocarpus siliculosus, and two glaucophyte species. We demonstrate that algal phytochromes are not limited to red and far-red responses. Instead, different algal phytochromes can sense orange, green, and even blue light. Characterization of these previously undescribed photosensors using CD spectroscopy supports a structurally heterogeneous chromophore in the far-red-absorbing photostate. Our study thus demonstrates that extensive spectral tuning of phytochromes has evolved in phylogenetically distinct lineages of aquatic photosynthetic eukaryotes.	[Rockwell, Nathan C.; Duanmu, Deqiang; Martin, Shelley S.; Lagarias, J. Clark] Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA; [Bachy, Charles; Worden, Alexandra Z.] Monterey Bay Aquarium Res Inst, Moss Landing, CA 95039 USA; [Price, Dana C.; Bhattacharya, Debashish] Rutgers State Univ, Inst Marine & Coastal Sci, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08903 USA; [Worden, Alexandra Z.] Canadian Inst Adv Res, Integrated Microbial Biodivers Program, Toronto, ON M5G 1Z8, Canada	Lagarias, JC (corresponding author), Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA.	jclagarias@ucdavis.edu	Worden, Alexandra Z./AAD-6567-2019; Lagarias, John Clark/L-3139-2013; Duanmu, Deqiang/AAZ-6909-2020; Bachy, Charles/L-1341-2019	Lagarias, John Clark/0000-0002-2093-0403; Duanmu, Deqiang/0000-0002-9365-362X; Bachy, Charles/0000-0001-8013-8066; Worden, Alexandra/0000-0002-9888-9324	National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 GM068552]; US Department of Agriculture National Institute of Food and Agriculture Hatch Project [CA-D*-MCB-4126-H]; National Science FoundationNational Science Foundation (NSF) [MGSP 0625440, MCB 0946258]; Department of DefenseUnited States Department of Defense [DE-SC0004765]; Packard FoundationThe David & Lucile Packard Foundation; GBMF Investigator award; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552, R01GM068552] Funding Source: NIH RePORTER	We thank the Gordon and Betty Moore Foundation (GBMF) Marine Microbial Eukaryote Transcriptome Sequencing Project for sequencing and assembling algal transcriptome contigs and S. Sudek and A. Reyes-Prieto for growing these algae. This work was supported by National Institutes of Health Grant R01 GM068552 and US Department of Agriculture National Institute of Food and Agriculture Hatch Project CA-D*-MCB-4126-H (to J.C.L.), National Science Foundation Grants MGSP 0625440 and MCB 0946258 (to D. B.), and Department of Defense Grant DE-SC0004765, the Packard Foundation, and a GBMF Investigator award (to A.Z.W.).	Armbrust EV, 2009, NATURE, V459, P185, DOI 10.1038/nature08057; Auldridge ME, 2011, CRIT REV BIOCHEM MOL, V46, P67, DOI 10.3109/10409238.2010.546389; Barone R., 2006, NATURALISTA SICILIAN, V30, P97; Bellini D, 2012, STRUCTURE, V20, P1436, DOI 10.1016/j.str.2012.06.002; BERKELMAN TR, 1986, ANAL BIOCHEM, V156, P194, DOI 10.1016/0003-2697(86)90173-9; BIRD CJ, 1983, AQUAT BOT, V16, P315, DOI 10.1016/0304-3770(83)90078-5; Borucki B, 2005, J PHYS CHEM B, V109, P629, DOI 10.1021/jp046515k; Borucki B, 2003, BIOCHEMISTRY-US, V42, P13684, DOI 10.1021/bi035511n; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Burgie ES, 2013, STRUCTURE, V21, P88, DOI 10.1016/j.str.2012.11.001; Burki F, 2012, P ROY SOC B-BIOL SCI, V279, P2246, DOI 10.1098/rspb.2011.2301; Casal JJ, 2013, ANNU REV PLANT BIOL, V64, P403, DOI 10.1146/annurev-arplant-050312-120221; Chen M, 2011, TRENDS CELL BIOL, V21, P664, DOI 10.1016/j.tcb.2011.07.002; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2013, P NATL ACAD SCI USA, V110, P5247, DOI 10.1073/pnas.1221259110; Dammeyer T, 2008, PHOTOCH PHOTOBIO SCI, V7, P1121, DOI 10.1039/b807209b; Duanmu D, 2013, P NATL ACAD SCI USA, V110, P3621, DOI 10.1073/pnas.1222375110; Essen LO, 2008, P NATL ACAD SCI USA, V105, P14709, DOI 10.1073/pnas.0806477105; Falciatore A, 2005, CURR TOP DEV BIOL, V68, P317, DOI 10.1016/S0070-2153(05)68011-8; Falk H., 1989, CHEM LINEAR OLIGOPYR; Fischer AJ, 2005, BIOCHEMISTRY-US, V44, P15203, DOI 10.1021/bi051633z; Fixen KR, 2014, P NATL ACAD SCI USA, V111, pE237, DOI 10.1073/pnas.1322410111; Franklin KA, 2010, J EXP BOT, V61, P11, DOI 10.1093/jxb/erp304; Gambetta GA, 2001, P NATL ACAD SCI USA, V98, P10566, DOI 10.1073/pnas.191375198; Hasegawa T, 1996, PHYCOLOGIA, V35, P170, DOI 10.2216/i0031-8884-35-2-170.1; HORI T, 1982, BOT MAG TOKYO, V95, P49, DOI 10.1007/BF02493410; Hu W, 2013, P NATL ACAD SCI USA, V110, P1542, DOI 10.1073/pnas.1221738110; Hughes J, 2013, ANNU REV PLANT BIOL, V64, P377, DOI 10.1146/annurev-arplant-050312-120045; Hughes J, 2010, BIOCHEM SOC T, V38, P710, DOI 10.1042/BST0380710; Ikeuchi M, 2008, PHOTOCH PHOTOBIO SCI, V7, P1159, DOI 10.1039/b802660m; Ishizuka T, 2011, BIOCHEMISTRY-US, V50, P953, DOI 10.1021/bi101626t; Jorissen HJMM, 2002, PHOTOCHEM PHOTOBIOL, V76, P457, DOI 10.1562/0031-8655(2002)076<0457:HEACOR>2.0.CO;2; Jouenne F, 2011, PROTIST, V162, P70, DOI 10.1016/j.protis.2010.04.005; Karl DM, 2012, P NATL ACAD SCI USA, V109, P1842, DOI 10.1073/pnas.1120312109; KIDD DG, 1990, J BIOL CHEM, V265, P7029; Kiirikki M, 1996, MAR BIOL, V127, P353, DOI 10.1007/BF00942120; Kim PW, 2012, CHEM PHYS LETT, V549, P86, DOI 10.1016/j.cplett.2012.08.044; LEMAUX PG, 1985, EMBO J, V4, P1911, DOI 10.1002/j.1460-2075.1985.tb03870.x; LITTS JC, 1983, J BIOL CHEM, V258, P1025; Marin B, 2010, PROTIST, V161, P304, DOI 10.1016/j.protis.2009.10.002; Mathews S, 2006, MOL ECOL, V15, P3483, DOI 10.1111/j.1365-294X.2006.03051.x; MOESTRUP O, 1983, Nordic Journal of Botany, V3, P609, DOI 10.1111/j.1756-1051.1983.tb01472.x; MOREL A, 1988, J GEOPHYS RES-OCEANS, V93, P10749, DOI 10.1029/JC093iC09p10749; Norton TA, 1968, HYDROBIOLOGIA, V40, P55; Price DC, 2012, SCIENCE, V335, P843, DOI 10.1126/science.1213561; Rockwell NC, 2008, BIOCHEMISTRY-US, V47, P7304, DOI 10.1021/bi800088t; Rockwell NC, 2006, ANNU REV PLANT BIOL, V57, P837, DOI 10.1146/annurev.arplant.56.032604.144208; Rockwell NC, 2012, BIOCHEMISTRY-US, V51, P9667, DOI 10.1021/bi3013565; Rockwell NC, 2012, BIOCHEMISTRY-US, V51, P3576, DOI 10.1021/bi300171s; Rockwell NC, 2012, BIOCHEMISTRY-US, V51, P1449, DOI 10.1021/bi201783j; Rockwell NC, 2011, P NATL ACAD SCI USA, V108, P11854, DOI 10.1073/pnas.1107844108; Rockwell NC, 2010, CHEMPHYSCHEM, V11, P1172, DOI 10.1002/cphc.200900894; Rockwell NC, 2009, P NATL ACAD SCI USA, V106, P6123, DOI 10.1073/pnas.0902370106; SCHUMACHER G. J., 1961, JOUR ELISHA MITCHED SCI SOC, V77, P274; Song C, 2014, J BIOL CHEM, V289, P2552, DOI 10.1074/jbc.M113.520031; Song C, 2011, BIOCHEMISTRY-US, V50, P10987, DOI 10.1021/bi201504a; Song C, 2011, P NATL ACAD SCI USA, V108, P3842, DOI 10.1073/pnas.1013377108; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; Strasser B, 2010, P NATL ACAD SCI USA, V107, P4776, DOI 10.1073/pnas.0910446107; Tabor JJ, 2011, J MOL BIOL, V405, P315, DOI 10.1016/j.jmb.2010.10.038; Thomsen HA, 1998, DEEP-SEA RES PT II, V45, P1687, DOI 10.1016/S0967-0645(98)80013-1; Throndsen J, 1997, PHYCOLOGIA, V36, P244, DOI 10.2216/i0031-8884-36-3-244.1; Timme RE, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0029696; Wagner JR, 2008, J BIOL CHEM, V283, P12212, DOI 10.1074/jbc.M709355200; Wagner JR, 2005, NATURE, V438, P325, DOI 10.1038/nature04118; Wang WJ, 2013, PLANTA, V237, P1123, DOI 10.1007/s00425-012-1831-7; Worden AZ, 2009, SCIENCE, V324, P268, DOI 10.1126/science.1167222; Wu SH, 1997, J BIOL CHEM, V272, P25700, DOI 10.1074/jbc.272.41.25700; Yang X, 2008, P NATL ACAD SCI USA, V105, P14715, DOI 10.1073/pnas.0806718105; Yang X, 2007, P NATL ACAD SCI USA, V104, P12571, DOI 10.1073/pnas.0701737104; Yang XJ, 2011, NATURE, V479, P428, DOI 10.1038/nature10506; Yeh KC, 1997, SCIENCE, V277, P1505, DOI 10.1126/science.277.5331.1505	72	108	108	0	79	NATL ACAD SCIENCES	WASHINGTON	2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA	0027-8424			P NATL ACAD SCI USA	Proc. Natl. Acad. Sci. U. S. A.	MAR 11	2014	111	10					3871	3876		10.1073/pnas.1401871111			6	Multidisciplinary Sciences	Science & Technology - Other Topics	AC5MK	WOS:000332564800055	24567382	Bronze, Green Accepted, Green Published			2021-04-07	
J	Sun, J; Wang, L; Wu, SX; Wang, XM; Xiao, JF; Chi, S; Liu, C; Ren, LF; Zhao, YH; Liu, T; Yu, J				Sun Jing; Wang Liang; Wu Shuangxiu; Wang Xumin; Xiao Jingfa; Chi Shan; Liu Cui; Ren Lufeng; Zhao Yuhui; Liu Tao; Yu Jun			Transcriptome-wide evolutionary analysis on essential brown algae (Phaeophyceae) in China	ACTA OCEANOLOGICA SINICA			English	Article						Phaeophyceae; transcriptome sequencing; multigene; phylogeny	RNA ISOLATION; EXPRESSION; PATHWAY; GENOME	Brown algae (Chromista, Ochrophyta, Phaeophyceae) are a large group of multicellular algae that play important roles in the ocean's ecosystem and biodiversity. However, poor molecular bases for studying their phylogenetic evolutions and novel metabolic characteristics have hampered progress in the field. In this study, we sequenced the de novo transcriptome of 18 major species of brown algae in China, covering six orders and seven families, using the high-throughput sequencing platform Illumina HiSeq 2000. From the transcriptome data of these 18 species and publicly available genome data of Ectocarpus siliculosus and Phaeodactylum tricornutum, we identified 108 nuclear-generated orthologous genes and clarified the phylogenetic relationships among these brown algae based on a multigene method. These brown algae could be separated into two clades: Clade Ishigeales-Dictyotales and Clade Ectocarpales-Laminariales-Desmarestiale-Fucales. The former was at the base of the phylogenetic tree, indicating its early divergence, while the latter was divided into two branches, with Order Fucales diverging from Orders Ectocarpales, Laminariales, and Desmarestiale. In our analysis of taxonomy-contentious species, Sargassum fusiforme and Saccharina sculpera were found to be closely related to genera Sargassum and Saccharina, respectively, while Petalonia fascia showed possible relation to genus Scytosiphon. The study provided molecular evidence for the phylogenetic taxonomy of brown algae.	[Sun Jing; Wang Liang; Wu Shuangxiu; Wang Xumin; Xiao Jingfa; Ren Lufeng; Zhao Yuhui; Yu Jun] Chinese Acad Sci, CAS Key Lab Genome Sci & Informat, Beijing Key Lab Genome & Precis Med Technol, Beijing Inst Gen, Beijing 100101, Peoples R China; [Chi Shan; Liu Cui; Liu Tao] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; [Sun Jing; Wang Liang; Wu Shuangxiu; Wang Xumin; Xiao Jingfa; Ren Lufeng; Yu Jun] Chinese Acad Sci, Beijing Key Lab Funct Gen Dao di Herbs, Beijing Inst Gen, Beijing 100101, Peoples R China; [Sun Jing; Wang Liang; Zhao Yuhui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China	Liu, T (corresponding author), Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China.	liutao@ouc.edu.cn; junyu@big.ac.cn			National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31140070, 31271397, 41206116]	The National Natural Science Foundation of China under contract Nos 31140070, 31271397 and 41206116; the algal transcriptome sequencing was supported by 1KP Project (www.onekp.com).	Alexeyenko A, 2006, BIOINFORMATICS, V22, pE9, DOI 10.1093/bioinformatics/btl213; Charrier B, 2012, TRENDS PLANT SCI, V17, P468, DOI 10.1016/j.tplants.2012.03.003; Cho GY, 2006, PHYCOLOGIA, V45, P512, DOI 10.2216/05-48.1; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coyer JA, 2011, MOL PHYLOGENET EVOL, V58, P283, DOI 10.1016/j.ympev.2010.11.015; Darriba D, 2011, BIOINFORMATICS, V27, P1164, DOI 10.1093/bioinformatics/btr088; Deng YY, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0039704; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Do CB, 2005, GENOME RES, V15, P330, DOI 10.1101/gr.2821705; Dorrell RG, 2011, EUKARYOT CELL, V10, P856, DOI 10.1128/EC.00326-10; Eom SH, 2012, FOOD CHEM TOXICOL, V50, P3251, DOI 10.1016/j.fct.2012.06.028; Flores-Moya A, 2002, J PHOTOCH PHOTOBIO B, V66, P134, DOI 10.1016/S1011-1344(02)00233-6; Ghangal R, 2009, PLANT PHYSIOL BIOCH, V47, P1113, DOI 10.1016/j.plaphy.2009.09.004; Green BR, 2011, PHOTOSYNTH RES, V107, P103, DOI 10.1007/s11120-010-9584-2; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Heinrich S, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0044342; Kawai H, 2005, PHYCOLOGIA, V44, P169, DOI 10.2216/0031-8884(2005)44[169:MAMPOP]2.0.CO;2; Lane CE, 2006, J PHYCOL, V42, P493, DOI 10.1111/j.1529-8817.2006.00204.x; Li RQ, 2008, BIOINFORMATICS, V24, P713, DOI 10.1093/bioinformatics/btn025; [李天勇 Li Tianyong], 2012, [海洋湖沼通报, Transactions of Oceanology and Limnology], P64; Lu MW, 2012, BMC GENOMICS, V13, DOI 10.1186/1471-2164-13-651; Luo RB, 2012, GIGASCIENCE, V1, DOI 10.1186/2047-217X-1-18; Moriya Y, 2007, NUCLEIC ACIDS RES, V35, pW182, DOI 10.1093/nar/gkm321; O'Brien KP, 2005, NUCLEIC ACIDS RES, V33, pD476, DOI 10.1093/nar/gki107; Pearson WR, 1997, GENOMICS, V46, P24, DOI 10.1006/geno.1997.4995; Pearson WR, 1996, METHOD ENZYMOL, V266, P227; PEARSON WR, 1988, P NATL ACAD SCI USA, V85, P2444, DOI 10.1073/pnas.85.8.2444; RHODES R G, 1973, Chesapeake Science, V14, P211, DOI 10.2307/1350610; Rokas A, 2005, MOL BIOL EVOL, V22, P1337, DOI 10.1093/molbev/msi121; SETCHELL WILLIAM ALBERT, 1931, HONG KONG NATURALIST, V2, P237; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Stamatakis A, 2005, BIOINFORMATICS, V21, P456, DOI 10.1093/bioinformatics/bti191; Stiger Valerie, 2003, Phycological Research, V51, P1; Xu J, 2010, PHYTOCHEM ANALYSIS, V21, P395, DOI 10.1002/pca.1205; Yao JT, 2009, J APPL PHYCOL, V21, P233, DOI 10.1007/s10811-008-9354-0; YOSHIDA T, 1990, Japanese Journal of Phycology, V38, P269; Zambounis A, 2012, MOL BIOL EVOL, V29, P1263, DOI 10.1093/molbev/msr296	37	3	4	2	21	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0253-505X	1869-1099		ACTA OCEANOL SIN	Acta Oceanol. Sin.	FEB	2014	33	2					13	19		10.1007/s13131-014-0436-3			7	Oceanography	Oceanography	AB2MQ	WOS:000331627200002					2021-04-07	
J	Wang, L; Wu, SX; Liu, T; Sun, J; Chi, S; Liu, C; Li, XG; Yin, JL; Wang, XM; Yu, J				Wang Liang; Wu Shuangxiu; Liu Tao; Sun Jing; Chi Shan; Liu Cui; Li Xingang; Yin Jinlong; Wang Xumin; Yu Jun			Endogenous viral elements in algal genomes	ACTA OCEANOLOGICA SINICA			English	Article						endogenous viral elements; algae; genome; transcriptome	ECTOCARPUS-SILICULOSUS; SEQUENCE; VIRUSES; ALIGNMENT; EVOLUTION; DATABASE	Endogenous viral elements (EVEs) are host-genomic fragments originated from viral genomes. They have been found universally in animal and plant genomes. Here we carried out a systematic screening and analysis of EVEs in algal genomes and found that EVEs commonly exist in algal genomes. We classified the EVE fragments into three categories according to the length of EVE fragments. Due to the probability of sequence similarity by chance, we ignored the potential function of medium-length EVE fragments. However, longlength EVE fragments probably had capability to encode protein domains or even entire proteins, and some short-length EVE fragments had high similarity with host's siRNA sequences and possibly served functions of small RNAs. Therefore, short and long EVE fragments might provide regulomic and proteomic novelty to the host's metabolism and adaptation. We also found several EVE fragments shared by more than 3 algal genomes. By phylogenetic analysis of the shared EVEs and their corresponding species, we found that the integration of viral fragments into host genomes was an ancient event, possibly before the divergence of Chlorophytes and Ochrophytes. Our findings show that there is a frequent genetic flow from viruses to algal genomes. Moreover, study on algal EVEs shed light on the virus-host interaction in large timescale and could also help us understand the balance of marine ecosystems.	[Wang Liang; Wu Shuangxiu; Sun Jing; Li Xingang; Yin Jinlong; Wang Xumin; Yu Jun] Chinese Acad Sci, CAS Key Lab Genome Sci & Informat, Beijing Key Lab Genome & Precis Med Technol, Beijing Inst Gen, Beijing 100101, Peoples R China; [Liu Tao; Chi Shan; Liu Cui] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; [Wang Liang; Wu Shuangxiu; Sun Jing; Li Xingang; Wang Xumin; Yu Jun] Chinese Acad Sci, Beijing Key Lab Funct Gen Dao di Herbs, Beijing Inst Gen, Beijing 100101, Peoples R China; [Wang Liang; Sun Jing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China	Wang, XM (corresponding author), Chinese Acad Sci, CAS Key Lab Genome Sci & Informat, Beijing Key Lab Genome & Precis Med Technol, Beijing Inst Gen, Beijing 100101, Peoples R China.	wangxm@big.ac.cn; junyu@big.ac.cn		Li, Xingang/0000-0003-0252-154X	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31140070, 31271397, 41206116]	The National Natural Science Foundation of China under contract Nos 31140070, 31271397 and 41206116; the algal transcriptome sequencing was supported by 1KP Project (www.onekp.com).	Alexeyenko A, 2006, BIOINFORMATICS, V22, pE9, DOI 10.1093/bioinformatics/btl213; Allen MJ, 2006, MOL BIOL EVOL, V23, P86, DOI 10.1093/molbev/msj010; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; BOGUSKI MS, 1993, NAT GENET, V4, P332, DOI 10.1038/ng0893-332; Brussaard CPD, 2004, J EUKARYOT MICROBIOL, V51, P125, DOI 10.1111/j.1550-7408.2004.tb00537.x; Cerutti H, 2011, EUKARYOT CELL, V10, P1164, DOI 10.1128/EC.05106-11; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Darriba D, 2011, BIOINFORMATICS, V27, P1164, DOI 10.1093/bioinformatics/btr088; Dean WE, 1998, GEOLOGY, V26, P535, DOI 10.1130/0091-7613(1998)026<0535:MASOCB>2.3.CO;2; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Delaroque N, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-110; Do CB, 2005, GENOME RES, V15, P330, DOI 10.1101/gr.2821705; Dunigan DD, 2006, VIRUS RES, V117, P119, DOI 10.1016/j.virusres.2006.01.024; Emerman M, 2010, PLOS BIOL, V8, DOI 10.1371/journal.pbio.1000301; Essoussi N, 2008, BIOINFORMATION, V2, P452; Fitzgerald LA, 2007, VIROLOGY, V358, P472, DOI 10.1016/j.virol.2006.08.033; Fuhrman JA, 1999, NATURE, V399, P541, DOI 10.1038/21119; Gifford R, 2003, VIRUS GENES, V26, P291, DOI 10.1023/A:1024455415443; Goodstein DM, 2012, NUCLEIC ACIDS RES, V40, pD1178, DOI 10.1093/nar/gkr944; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Katzourakis A, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1001191; Kent WJ, 2002, GENOME RES, V12, P656, DOI [10.1101/gr.229202. Article published online before March 2002, 10.1101/gr.229202]; King AMQ, 2012, VIRUS TAXONOMY, P1327; Langmead B, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-3-r25; Lavrukhin OV, 2000, J BIOL CHEM, V275, P6915, DOI 10.1074/jbc.275.10.6915; Meints RH, 2008, J VIROL, V82, P1407, DOI 10.1128/JVI.01983-07; Mette MF, 2002, EMBO J, V21, P461, DOI 10.1093/emboj/21.3.461; Mistry J, 2013, NUCLEIC ACIDS RES, V41, DOI [10.1093/nar/gkt263, 10.1093/nar/gkt1223]; Monier A, 2009, GENOME RES, V19, P1441, DOI 10.1101/gr.091686.109; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; O'Brien KP, 2005, NUCLEIC ACIDS RES, V33, pD476, DOI 10.1093/nar/gki107; Raoult D, 2004, SCIENCE, V306, P1344, DOI 10.1126/science.1101485; Ribet D, 2008, GENOME RES, V18, P597, DOI 10.1101/gr.073486.107; Staginnus C, 2006, TRENDS PLANT SCI, V11, P485, DOI 10.1016/j.tplants.2006.08.008; Stamatakis A, 2006, BIOINFORMATICS, V22, P2688, DOI 10.1093/bioinformatics/btl446; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Wilson WH, 2005, SCIENCE, V309, P1090, DOI 10.1126/science.1113109; Wommack KE, 2000, MICROBIOL MOL BIOL R, V64, P69, DOI 10.1128/MMBR.64.1.69-114.2000; Yanai-Balser GM, 2010, J VIROL, V84, P532, DOI 10.1128/JVI.01698-09; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847	40	7	7	0	14	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	0253-505X	1869-1099		ACTA OCEANOL SIN	Acta Oceanol. Sin.	FEB	2014	33	2					102	107		10.1007/s13131-014-0446-1			6	Oceanography	Oceanography	AB2MQ	WOS:000331627200012					2021-04-07	
J	Miller, EP; Bottger, LH; Weerasinghe, AJ; Crumbliss, AL; Matzanke, BF; Meyer-Klaucke, W; Kupper, FC; Carrano, CJ				Miller, Eric P.; Boettger, Lars H.; Weerasinghe, Aruna J.; Crumbliss, Alvin L.; Matzanke, Berthold F.; Meyer-Klaucke, Wolfram; Kuepper, Frithjof C.; Carrano, Carl J.			Surface-bound iron: a metal ion buffer in the marine brown alga Ectocarpus siliculosus?	JOURNAL OF EXPERIMENTAL BOTANY			English	Article						Apoplast; brown algae; cell wall; energy-dispersive X-ray analysis; histochemistry; iron; Mssbauer spectroscopy; surface binding; X-ray absorption	CELL-SURFACE; BIOSORPTION; MECHANISMS; PACIFIC; LIGANDS; BINDING; STORAGE	Although the iron uptake and storage mechanisms of terrestrial/higher plants have been well studied, the corresponding systems in marine algae have received far less attention. Studies have shown that while some species of unicellular algae utilize unique mechanisms of iron uptake, many acquire iron through the same general mechanisms as higher plants. In contrast, the iron acquisition strategies of the multicellular macroalgae remain largely unknown. This is especially surprising since many of these organisms represent important ecological and evolutionary niches in the coastal marine environment. It has been well established in both laboratory and environmentally derived samples, that a large amount of iron can be non-specifically adsorbed to the surface of marine algae. While this phenomenon is widely recognized and has prompted the development of experimental protocols to eliminate its contribution to iron uptake studies, its potential biological significance as a concentrated iron source for marine algae is only now being recognized. This study used an interdisciplinary array of techniques to explore the nature of the extensive and powerful iron binding on the surface of both laboratory and environmental samples of the marine brown alga Ectocarpus siliculosus and shows that some of this surface-bound iron is eventually internalized. It is proposed that the surface-binding properties of E. siliculosus allow it to function as a quasibiological metal ion buffer, allowing iron uptake under the widely varying external iron concentrations found in coastal marine environments.	[Miller, Eric P.; Carrano, Carl J.] San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA; [Boettger, Lars H.; Matzanke, Berthold F.] Med Univ Lubeck, Isotopes Lab, Sect Nat Sci, D-23538 Lubeck, Germany; [Weerasinghe, Aruna J.; Crumbliss, Alvin L.] Duke Univ, Dept Chem, Durham, NC 27708 USA; [Meyer-Klaucke, Wolfram] DESY, European Mol Biol Lab, Hamburg Unit, D-22607 Hamburg, Germany; [Kuepper, Frithjof C.] Univ Aberdeen, Oceanlab, Newburgh AB41 6AA, Scotland	Carrano, CJ (corresponding author), San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA.	carrano@sciences.sdsu.edu	Meyer-Klaucke, Wolfram/G-1148-2010	Kuepper, Frithjof/0000-0003-1273-7109	joint USA-Germany International Chemistry Collaboration (ICC) [NSF CHE-0924313, DFG Ma 916/20-1]; NSFNational Science Foundation (NSF) [CHE-0809466]; Duke University; European CommunityEuropean Commission [2277.88]; Scottish Funding Council [HR09011]	This work is supported by joint USA-Germany International Chemistry Collaboration (ICC) grant: NSF CHE-0924313 to CJC and DFG Ma 916/20-1 to BFM. ALC thanks the NSF (CHE-0809466) and Duke University for financial support. Furthermore, the authors acknowledge the support of the European Community research infrastructure action under the FP7 'capacities' specific programme ASSEMBLE (grant no. 2277.88). EPM and CJC thank Dr Steve Barlow, SDSU Microscopy Center for help with the histochemistry and microscopy and Prof Kathy Barbeau and her students for providing oligotrophic open ocean water. Finally, the MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant no. HR09011) is gratefully acknowledged for supporting FCK.	Amin SA, 2007, J AM CHEM SOC, V129, P478, DOI 10.1021/ja067369u; Andersen R, 2005, ALGAL CULTURING TECH; Benvegnu T, 2010, TOP CURR CHEM, V294, P143, DOI 10.1007/128_2010_48; Bottger LH, 2012, J EXP BOT, V63, P5763, DOI 10.1093/jxb/ers225; Boye M, 2000, MAR CHEM, V70, P277, DOI 10.1016/S0304-4203(00)00032-3; Bringezu S, 2009, SUSTAINABLE PRODUCTI, V118; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chase Z, 2002, J GEOPHYS RES-OCEANS, V107, DOI 10.1029/2001JC000987; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Davis TA, 2003, WATER RES, V37, P4311, DOI 10.1016/S0043-1354(03)00293-8; DZIOBKOWSKI CT, 1981, INORG CHEM, V20, P671, DOI 10.1021/ic50217a007; HUDSON RJM, 1989, LIMNOL OCEANOGR, V34, P1113, DOI 10.4319/lo.1989.34.6.1113; Hutchins DA, 1999, AQUAT MICROB ECOL, V19, P129, DOI 10.3354/ame019129; Ingall ED, 2013, NAT COMMUN, V4, DOI 10.1038/ncomms2981; Kelly LW, 2012, ISME J, V6, P638, DOI 10.1038/ismej.2011.114; Korbas M, 2006, REV SCI INSTRUM, V77, DOI 10.1063/1.2209954; Kupper FC, 1998, PLANTA, V207, P163, DOI 10.1007/s004250050469; MABEAU S, 1987, J EXP BOT, V38, P1573, DOI 10.1093/jxb/38.9.1573; MARTIN JH, 1988, NATURE, V331, P341, DOI 10.1038/331341a0; Meguro R, 2007, ARCH HISTOL CYTOL, V70, P1, DOI 10.1679/aohc.70.1; Milligan AJ, 2009, MAR CHEM, V114, P31, DOI 10.1016/j.marchem.2009.03.003; Morrissey J, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00043; Morup S., 2011, MOSSBAUER SPECTROSCO, P201; Muller DG, 2008, CAH BIOL MAR, V49, P59; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Philpott CC, 2006, BBA-MOL CELL RES, V1763, P636, DOI 10.1016/j.bbamcr.2006.05.008; Raize O, 2004, BIOTECHNOL BIOENG, V87, P451, DOI 10.1002/bit.20136; Ravel B, 2005, J SYNCHROTRON RADIAT, V12, P537, DOI 10.1107/S0909049505012719; Roschzttardtz H, 2009, PLANT PHYSIOL, V151, P1329, DOI 10.1104/pp.109.144444; Rubin M, 2011, NAT GEOSCI, V4, P529, DOI 10.1038/NGEO1181; RUE EL, 1995, MAR CHEM, V50, P117, DOI 10.1016/0304-4203(95)00031-L; Sutak R, 2012, PLANT PHYSIOL, V160, P2271, DOI 10.1104/pp.112.204156; Sutak R, 2010, PLANT PHYSIOL, V154, P991, DOI 10.1104/pp.110.159947; Tovar-Sanchez A, 2003, MAR CHEM, V82, P91, DOI 10.1016/S0304-4203(03)00054-9; TRICK CG, 1983, SCIENCE, V219, P306, DOI 10.1126/science.219.4582.306; Usov AI, 2009, RUSS CHEM REV+, V78, P785, DOI 10.1070/RC2009v078n08ABEH004063; Vertes DA, 2010, BIOMASS BIOFUELS STR; Wellenreuther G, 2009, J PHYS CONF SER, V190, DOI 10.1088/1742-6596/190/1/012033	38	10	10	0	25	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0022-0957	1460-2431		J EXP BOT	J. Exp. Bot.	FEB	2014	65	2					585	594		10.1093/jxb/ert406			10	Plant Sciences	Plant Sciences	AA5EQ	WOS:000331119300018	24368501	Green Published, Bronze			2021-04-07	
J	Groisillier, A; Shao, ZR; Michel, G; Goulitquer, S; Bonin, P; Krahulec, S; Nidetzky, B; Duan, DL; Boyen, C; Tonon, T				Groisillier, Agns; Shao, Zhanru; Michel, Gurvan; Goulitquer, Sophie; Bonin, Patricia; Krahulec, Stefan; Nidetzky, Bernd; Duan, Delin; Boyen, Catherine; Tonon, Thierry			Mannitol metabolism in brown algae involves a new phosphatase family	JOURNAL OF EXPERIMENTAL BOTANY			English	Article						Carbon storage; Ectocarpus siliculosus; HAD (haloacid dehalogenase) superfamily; mannitol; phosphatases; primary; metabolism	ECTOCARPUS-SILICULOSUS; EXPRESSION; EVOLUTION; INSIGHTS; ORIGIN; DEHYDROGENASE; SUPERFAMILY; DIVERSITY; SEQUENCE; CLONING	Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of these is the mannitol cycle, which plays a central role in their physiology, as mannitol acts as carbon storage, osmoprotectant, and antioxidant. This polyol is derived directly from the photoassimilate fructose-6-phosphate via the action of a mannitol-1-phosphate dehydrogenase and a mannitol-1-phosphatase (M1Pase). Genome analysis of the brown algal model Ectocarpus siliculosus allowed identification of genes potentially involved in the mannitol cycle. Among these, two genes coding for haloacid dehalogenase (HAD)-like enzymes were suggested to correspond to M1Pase activity, and thus were named EsM1Pase1 and EsM1Pase2, respectively. To test this hypothesis, both genes were expressed in Escherichia coli. Recombinant EsM1Pase2 was shown to hydrolyse the phosphate group from mannitol-1-phosphate to produce mannitol but was not active on the hexose monophosphates tested. Gene expression analysis showed that transcription of both E. siliculosus genes was under the influence of the diurnal cycle. Sequence analysis and three-dimensional homology modelling indicated that EsM1Pases, and their orthologues in Prasinophytes, should be seen as founding members of a new family of phosphatase with original substrate specificity within the HAD superfamily of proteins. This is the first report describing the characterization of a gene encoding M1Pase activity in photosynthetic organisms.	[Groisillier, Agns; Shao, Zhanru; Michel, Gurvan; Bonin, Patricia; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France; [Groisillier, Agns; Shao, Zhanru; Michel, Gurvan; Bonin, Patricia; Boyen, Catherine; Tonon, Thierry] CNRS, Biol Stn, UMR Marine Plants & Biomol 7139, F-29680 Roscoff, France; [Shao, Zhanru; Duan, Delin] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Goulitquer, Sophie] CNRS, Plate Forme MetaboMER, F-29680 Roscoff, France; [Goulitquer, Sophie] UPMC, FR2424, Biol Stn, F-29680 Roscoff, France; [Krahulec, Stefan; Nidetzky, Bernd] Graz Univ Technol, Inst Biotechnol & Biochem Engn, A-8010 Graz, Austria	Tonon, T (corresponding author), Univ Paris 06, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France.	tonon@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; Patricia, Bonin/AAA-4462-2021; Duan, Delin/G-9002-2011	Tonon, Thierry/0000-0002-1454-6018; Patricia, Bonin/0000-0002-4194-7343; Nidetzky, Bernd/0000-0002-5030-2643; MICHEL, Gurvan/0000-0002-3009-6205	Emergence-UPMC research programme; project IDEALG 'Investissements d'avenir, Biotechnologies-Bioresources'French National Research Agency (ANR) [ANR-10-BTBR-04]	The authors thank Laurence Dartevelle for cultivation of E. siliculosus, Dr Fanny Gaillard for MS analyses within the METABOMER facilities, Sylvie Rousvoal and Dr Simon M. Dittami for providing E. siliculosus diurnal samples, and the latter for critical reading of the manuscript. ZS was hosted at the Station Biologique de Roscoff in the framework of the Joint Doctoral Promotion Programme CNRS-CAS 2010. PB received a PhD grant by the Emergence-UPMC 2011 research programme. This work also benefited from the support of the project IDEALG (ANR-10-BTBR-04) 'Investissements d'avenir, Biotechnologies-Bioresources'.	Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; Burroughs AM, 2006, J MOL BIOL, V361, P1003, DOI 10.1016/j.jmb.2006.06.049; Caparros-Martin JA, 2013, PLANTA, V237, P943, DOI 10.1007/s00425-012-1809-5; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Conde A, 2011, PLANT CELL PHYSIOL, V52, P1766, DOI 10.1093/pcp/pcr121; DeLano WL, 2005, DRUG DISCOV TODAY, V10, P213, DOI 10.1016/S1359-6446(04)03363-X; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Webb Benjamin, 2016, Curr Protoc Protein Sci, V86, DOI [10.1002/0471250953.bi0506s15, 10.1002/0471140864.ps0209s50, 10.1002/cpbi.3, 10.1002/cpps.20]; Gouet P, 2003, NUCLEIC ACIDS RES, V31, P3320, DOI 10.1093/nar/gkg556; GRANT CR, 1981, PHYTOCHEMISTRY, V20, P1505, DOI 10.1016/S0031-9422(00)98521-2; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Groisillier A, 2010, MICROB CELL FACT, V9, DOI 10.1186/1475-2859-9-45; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; IKAWA T, 1972, PLANT CELL PHYSIOL, V13, P1017; Iwamoto K, 2005, MAR BIOTECHNOL, V7, P407, DOI 10.1007/s10126-005-0029-4; Iwamoto K, 2001, MAR BIOTECHNOL, V3, P493, DOI 10.1007/s10126-001-0068-4; KARSTEN U, 1992, J PLANT PHYSIOL, V140, P292, DOI 10.1016/S0176-1617(11)81081-3; Karsten U, 1997, PLANTA, V201, P173, DOI 10.1007/BF01007701; Karsten U, 1999, J PHYCOL, V35, P967, DOI 10.1046/j.1529-8817.1999.3550967.x; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Krahulec S, 2008, CARBOHYD RES, V343, P1414, DOI 10.1016/j.carres.2008.04.011; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Liberator P, 1998, J BIOL CHEM, V273, P4237, DOI 10.1074/jbc.273.7.4237; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; RICHTER DFE, 1987, PLANTA, V170, P528, DOI 10.1007/BF00402987; Ridder IS, 1999, BIOCHEM J, V339, P223, DOI 10.1042/0264-6021:3390223; Rousvoal S, 2011, PLANTA, V233, P261, DOI 10.1007/s00425-010-1295-6; RUMPHO ME, 1983, PLANT PHYSIOL, V73, P869, DOI 10.1104/pp.73.4.869; Schmatz DM, 1997, PARASITOLOGY, V114, pS81; Seifried A, 2013, FEBS J, V280, P549, DOI 10.1111/j.1742-4658.2012.08633.x; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Stoop JMH, 1996, TRENDS PLANT SCI, V1, P139, DOI 10.1016/S1360-1385(96)80048-3; Studier FW, 2005, PROTEIN EXPRES PURIF, V41, P207, DOI 10.1016/j.pep.2005.01.016; Thomas F, 2012, ENVIRON MICROBIOL, V14, P2379, DOI 10.1111/j.1462-2920.2012.02751.x; Van Den Daele K, 2006, EUROPEAN JOURNAL OF, V41, P1; Wei N, 2013, TRENDS BIOTECHNOL, V31, P70, DOI 10.1016/j.tibtech.2012.10.009; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075	43	37	38	0	31	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0022-0957	1460-2431		J EXP BOT	J. Exp. Bot.	FEB	2014	65	2					559	570		10.1093/jxb/ert405			12	Plant Sciences	Plant Sciences	AA5EQ	WOS:000331119300016	24323504	Bronze			2021-04-07	
J	Stevens, K; Weynberg, K; Bellas, C; Brown, S; Brownlee, C; Brown, MT; Schroeder, DC				Stevens, Kim; Weynberg, Karen; Bellas, Christopher; Brown, Sonja; Brownlee, Colin; Brown, Murray T.; Schroeder, Declan C.			A Novel Evolutionary Strategy Revealed in the Phaeoviruses	PLOS ONE			English	Article							MARINE VIRUSES; DNA VIRUS; ECTOCARPUS-SILICULOSUS; ALGAL VIRUS; BROWN-ALGAE; PHAEOPHYCEAE; GENOME; COCCOLITHOVIRUS; FASCICULATUS; INFECTION	Phaeoviruses infect the brown algae, which are major contributors to primary production of coastal waters and estuaries. They exploit a Persistent evolutionary strategy akin to a K-selected life strategy via genome integration and are the only known representatives to do so within the giant algal viruses that are typified by r-selected Acute lytic viruses. In screening the genomes of five species within the filamentous brown algal lineage, here we show an unprecedented diversity of viral gene sequence variants especially amongst the smaller phaeoviral genomes. Moreover, one variant shares features from both the two major sub-groups within the phaeoviruses. These phaeoviruses have exploited the reduction of their giant dsDNA genomes and accompanying loss of DNA proofreading capability, typical of an Acute life strategist, but uniquely retain a Persistent life strategy.	[Stevens, Kim; Weynberg, Karen; Bellas, Christopher; Brown, Sonja; Brownlee, Colin; Schroeder, Declan C.] Marine Biol Assoc UK, Cell & Mol Dept, Plymouth, Devon, England; [Stevens, Kim; Weynberg, Karen; Bellas, Christopher; Brown, Sonja; Brownlee, Colin; Brown, Murray T.] Univ Plymouth, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England	Schroeder, DC (corresponding author), Marine Biol Assoc UK, Cell & Mol Dept, Plymouth, Devon, England.	dsch@mba.ac.uk	Schroeder, Declan C./O-9131-2019; Weynberg, Karen/AAW-1657-2020; Bellas, Christopher M/C-6292-2015; Brown, Murray/K-5291-2014	Schroeder, Declan C./0000-0001-5991-2838; Weynberg, Karen/0000-0002-9856-2137; Bellas, Christopher/0000-0001-5084-7830; Brown, Murray/0000-0003-2655-8611	INTERREG programme France (Channel) - England, MARINEXUS [1956/4073]; Plymouth University	This research was funded by INTERREG programme France (Channel) - England, MARINEXUS (Ref: 1956/4073) and Plymouth University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	BERGH O, 1989, NATURE, V340, P467, DOI 10.1038/340467a0; Brussaard CPD, 2008, ISME J, V2, P575, DOI 10.1038/ismej.2008.31; Claas ECJ, 1998, LANCET, V351, P472, DOI 10.1016/S0140-6736(97)11212-0; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Daee DL, 2010, P NATL ACAD SCI USA, V107, P157, DOI 10.1073/pnas.0907526106; Delaroque N, 2003, J MOL EVOL, V57, P613, DOI 10.1007/s00239-003-2501-y; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Elde NC, 2012, CELL, V150, P831, DOI 10.1016/j.cell.2012.05.049; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; Fuhrman JA, 1999, NATURE, V399, P541, DOI 10.1038/21119; Gao F, 1999, NATURE, V397, P436, DOI 10.1038/17130; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Maier I, 1997, J PHYCOL, V33, P838, DOI 10.1111/j.0022-3646.1997.00838.x; Martin SJ, 2012, SCIENCE, V336, P1304, DOI 10.1126/science.1220941; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Preston BD, 2010, SEMIN CANCER BIOL, V20, P281, DOI 10.1016/j.semcancer.2010.10.009; PROCTOR LM, 1990, NATURE, V343, P60, DOI 10.1038/343060a0; Roossinck MJ, 2010, PHILOS T R SOC B, V365, P1899, DOI 10.1098/rstb.2010.0057; Schroeder DC, 2002, ARCH VIROL, V147, P1685, DOI 10.1007/s00705-002-0841-3; Schroeder DC, 2011, MICROBIAL BOT HOST S, V1, P205; Schroeder DC, 2009, VIROLOGY, V384, P223, DOI 10.1016/j.virol.2008.10.040; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Starr RC, 1993, J PHYCOL S, V29, P90; SUTTLE CA, 1990, NATURE, V347, P467, DOI 10.1038/347467a0; Suttle CA, 2005, NATURE, V437, P356, DOI 10.1038/nature04160; Suttle CA, 2007, NAT REV MICROBIOL, V5, P801, DOI 10.1038/nrmicro1750; Villarreal LP, 2000, VIROLOGY, V272, P1, DOI 10.1006/viro.2000.0381; Wilson WH, 2005, SCIENCE, V309, P1090, DOI 10.1126/science.1113109	30	7	8	0	21	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	JAN 21	2014	9	1							e86040	10.1371/journal.pone.0086040			7	Multidisciplinary Sciences	Science & Technology - Other Topics	297GT	WOS:000330244500170	24465858	DOAJ Gold, Green Published, Green Accepted			2021-04-07	
J	Billoud, B; Nehr, Z; Le Bail, A; Charrier, B				Billoud, Bernard; Nehr, Zofia; Le Bail, Aude; Charrier, Benedicte			Computational prediction and experimental validation of microRNAs in the brown alga Ectocarpus siliculosus	NUCLEIC ACIDS RESEARCH			English	Article							EXPRESSION ANALYSIS; REGULATORY ROLES; GENOME SEQUENCE; SMALL RNAS; GENES; IDENTIFICATION; TARGET; EVOLUTION; REVEALS; PROTEIN	We used an in silico approach to predict microRNAs (miRNAs) genome-wide in the brown alga Ectocarpus siliculosus. As brown algae are phylogenetically distant from both animals and land plants, our approach relied on features shared by all known organisms, excluding sequence conservation, genome localization and pattern of base-pairing with the target. We predicted between 500 and 1500 miRNAs candidates, depending on the values of the energetic parameters used to filter the potential precursors. Using quantitative polymerase chain reaction assays, we confirmed the existence of 22 miRNAs among 72 candidates tested, and of 8 predicted precursors. In addition, we compared the expression of miRNAs and their precursors in two life cycle states (sporophyte, gametophyte) and under salt stress. Several miRNA precursors, Argonaute and DICER messenger RNAs were differentially expressed in these conditions. Finally, we analyzed the gene organization and the target functions of the predicted candidates. This showed that E. siliculosus miRNA genes are, like plant miRNA genes, rarely clustered and, like animal miRNA genes, often located in introns. Among the predicted targets, several widely conserved functional domains are significantly overrepresented, like kinesin, nucleotide-binding/APAF-1, R proteins and CED-4 (NB-ARC) and tetratricopeptide repeats. The combination of computational and experimental approaches thus emphasizes the originality of molecular and cellular processes in brown algae.	[Billoud, Bernard; Nehr, Zofia; Le Bail, Aude; Charrier, Benedicte] Univ Paris 06, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29688 Roscoff, France; [Billoud, Bernard; Nehr, Zofia; Le Bail, Aude; Charrier, Benedicte] CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29688 Roscoff, France	Billoud, B (corresponding author), Univ Paris 06, UMR Vegetaux Marins & Biomol 7139, Biol Stn, CS 90074, F-29688 Roscoff, France.	Bernard.Billoud@sb-roscoff.fr		Charrier, Benedicte/0000-0001-5721-1640; Billoud, Bernard/0000-0002-5140-8087	French Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Universite Pierre et Marie Curie	Funding for open access charge: French Centre National de la Recherche Scientifique and Universite Pierre et Marie Curie.	Adai A, 2005, GENOME RES, V15, P78, DOI 10.1101/gr.2908205; Allmer Jens, 2012, Frontiers in Genetics, V3, P209, DOI 10.3389/fgene.2012.00209; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Axtell MJ, 2011, GENOME BIOL, V12, DOI 10.1186/gb-2011-12-4-221; Baxter L, 2010, SCIENCE, V330, P1549, DOI 10.1126/science.1195203; Bologna NG, 2013, BRIEF FUNCT GENOMICS, V12, P37, DOI 10.1093/bfgp/els050; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Brodersen P, 2009, NAT REV MOL CELL BIO, V10, P141, DOI 10.1038/nrm2619; Cakir M., 2010, TOXOPLASMA GONDII, V5, P31; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cui X, 2009, GENE, V431, P61, DOI 10.1016/j.gene.2008.11.016; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Fiuza UM, 2007, J ENDOCRINOL, V194, P459, DOI 10.1677/JOE-07-0242; Frankel LB, 2008, J BIOL CHEM, V283, P1026, DOI 10.1074/jbc.M707224200; Griffiths-Jones S, 2008, NUCLEIC ACIDS RES, V36, pD154, DOI 10.1093/nar/gkm952; Gu Shuo, 2010, Silence, V1, P11, DOI 10.1186/1758-907X-1-11; Haas BJ, 2009, NATURE, V461, P393, DOI 10.1038/nature08358; Hellemans J, 2007, GENOME BIOL, V8, DOI 10.1186/gb-2007-8-2-r19; HOFACKER IL, 1994, MONATSH CHEM, V125, P167, DOI 10.1007/BF00818163; Huang AY, 2011, BMC GENOMICS, V12, DOI 10.1186/1471-2164-12-337; Hutvagner G, 2002, SCIENCE, V297, P2056, DOI 10.1126/science.1073827; Jones-Rhoades MW, 2006, ANNU REV PLANT BIOL, V57, P19, DOI 10.1146/annurev.arplant.57.032905.105218; Kapoor M, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-451; KAWAI H, 1995, J PHYCOL, V31, P306, DOI 10.1111/j.0022-3646.1995.00306.x; Khraiwesh B, 2012, BBA-GENE REGUL MECH, V1819, P137, DOI 10.1016/j.bbagrm.2011.05.001; Kisliouk T., 2010, EUR J NEUROSCI, V33, P224; Loong SNK, 2007, RNA, V13, P170, DOI 10.1261/rna.223807; Lai EC, 2003, GENOME BIOL, V4, DOI 10.1186/gb-2003-4-7-r42; Lau NC, 2001, SCIENCE, V294, P858, DOI 10.1126/science.1065062; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lee RC, 2001, SCIENCE, V294, P862, DOI 10.1126/science.1065329; Levesque CA, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-7-r73; Li GR, 2010, J BIOL CHEM, V285, P5461, DOI 10.1074/jbc.M109.037127; Lim LP, 2005, NATURE, V433, P769, DOI 10.1038/nature03315; Lim LP, 2003, GENE DEV, V17, P991, DOI 10.1101/gad.1074403; Lindow M, 2007, PLOS COMPUT BIOL, V3, P2379, DOI 10.1371/journal.pcbi.0030238; Lindow M, 2007, DNA CELL BIOL, V26, P339, DOI 10.1089/dna.2006.0551; Liu JD, 2004, SCIENCE, V305, P1437, DOI 10.1126/science.1102513; Liu QP, 2009, FUNCT INTEGR GENOMIC, V9, P277, DOI 10.1007/s10142-009-0111-5; Lowe TM, 1997, NUCLEIC ACIDS RES, V25, P955, DOI 10.1093/nar/25.5.955; Mathelier A, 2010, BIOINFORMATICS, V26, P2226, DOI 10.1093/bioinformatics/btq329; Mendes ND, 2009, NUCLEIC ACIDS RES, V37, P2419, DOI 10.1093/nar/gkp145; Merchan F, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-12-r136; Mestdagh P, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r64; Ng KLS, 2007, BIOINFORMATICS, V23, P1321, DOI 10.1093/bioinformatics/btm026; Paddy MR, 1998, AM J HUM GENET, V63, P305, DOI 10.1086/301989; Phillips N, 2008, J PHYCOL, V44, P394, DOI 10.1111/j.1529-8817.2008.00473.x; Pillai RS, 2004, RNA, V10, P1518, DOI 10.1261/rna.7131604; R Development Core Team, 2010, R LANG ENV STAT COMP; Rodriguez A, 2004, GENOME RES, V14, P1902, DOI 10.1101/gr.2722704; Shi R, 2005, BIOTECHNIQUES, V39, P519, DOI 10.2144/000112010; Shomron N, 2009, J BIOMED BIOTECHNOL, DOI 10.1155/2009/594738; Stark A, 2003, PLOS BIOL, V1, P397, DOI 10.1371/journal.pbio.0000060; Tikhonenko I, 2009, EUKARYOT CELL, V8, P723, DOI 10.1128/EC.00018-09; Vallejo DM, 2011, EMBO J, V30, P756, DOI 10.1038/emboj.2010.358; Vasudevan S, 2007, SCIENCE, V318, P1931, DOI 10.1126/science.1149460; Vazquez F, 2008, NUCLEIC ACIDS RES, V36, P6429, DOI 10.1093/nar/gkn670; Watanabe Y, 2006, GENE, V365, P2, DOI 10.1016/j.gene.2005.09.035; Wiesen JL, 2009, MOL IMMUNOL, V46, P1222, DOI 10.1016/j.molimm.2008.11.012; Yoon HS, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-14; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847; Zhou X, 2009, GENETICA, V137, P159, DOI 10.1007/s10709-009-9378-7; Zhu QH, 2008, GENOME RES, V18, P1456, DOI 10.1101/gr.075572.107	67	13	13	1	17	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0305-1048	1362-4962		NUCLEIC ACIDS RES	Nucleic Acids Res.	JAN	2014	42	1					417	429		10.1093/nar/gkt856			13	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	AA5KF	WOS:000331136000039	24078085	DOAJ Gold, Green Published			2021-04-07	
J	Deng, YY; Wang, XL; Guo, H; Duan, DL				Deng, Yunyan; Wang, Xiuliang; Guo, Hui; Duan, Delin			A trehalose-6-phosphate synthase gene from Saccharina japonica (Laminariales, Phaeophyceae)	MOLECULAR BIOLOGY REPORTS			English	Article						CBM20 domain; Drought adaption; Saccharina japonica; Trehalose-6-phosphate synthase; TPS domain; TPP domain	STARCH BINDING DOMAIN; TREHALOSE METABOLISM; ESCHERICHIA-COLI; BIOSYNTHESIS; EXPRESSION; EVOLUTION; REVEALS; GENOME; TRANSCRIPTOME; FERMENTATION	The full-length cDNA sequence of a trehalose-6-phosphate synthase gene from Saccharina japonica (designated as SjaTPS) (Accession: KC578568) was isolated based on homologous cloning and RACE-PCR. It was 4,127 bp, with 320 bp 5'-UTR, 21 bp 3'-UTR, and open reading frame (ORF) of 3,786 bp. The deduced 1,261 amino acids characterized with predicted molecular weight of 137.84 kDa and theoretical isoelectric point of 7.12. The SjaTPS had one N-terminal CBM20 (family 20 carbohydrate-binding module) domain, one TPS domain (trehalose-6-phosphate synthase) in the middle region and a single TPP (trehalose-6-phosphate phosphatase) domain near the C-terminus. Structural analysis suggested that the SjaTPS putatively functioned as trehalose-6-phosphate synthase, and might be related to laminaran metabolism in S. japonica. Homology analysis indicated that the SjaTPS shared 49-70 % similarities with the 13 known TPS sequences of other algae; only 55 % amino acid similarities were detected between SjaTPS and the previously reported TPS sequence of S. japonica (Accession: DQ666325). Phylogenetic analysis revealed close affinity between SjaTPS and TPS of brown alga Ectocarpus siliculosus (Accession: CBJ29609). Transcriptional analysis showed that desiccation greatly enhanced SjaTPS expression and the maximum appeared at 3 h, which was about 300-fold compared to that of the start, implied that SjaTPS was involved with drought adaption in kelp. In vitro expression of SjaTPS showed that one distinct band existed at similar to 115 kDa, and western blot detection proved that it was positive to the anti-His antibody with high specificity. Our results increased the knowledge of trehalose-6-phosphate synthase properties in S. japonica and also important for better understanding the role trehalose plays in kelp abiotic tolerance for adaption to the sublittoral habitats.	[Deng, Yunyan; Wang, Xiuliang; Guo, Hui; Duan, Delin] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Guo, Hui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China	Duan, DL (corresponding author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.	maimai0328@126.com; dlduan@qdio.ac.cn	Duan, Delin/G-9002-2011	Wang, Xiuliang/0000-0003-4262-0083	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [40976085]; Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project; National High Tech 863 ProjectNational High Technology Research and Development Program of China [2012AA10A406]	This research was supported by National Natural Science Foundation of China (No. 40976085) and Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project and National High Tech 863 Project (2012AA10A406). Sincerely thanks are due to Lin Xiao, Jin Zhao, Ge Liu for their help with the experiments. The authors acknowledged the anonymous reviewers for the critical comments and suggestions for the manuscript.	ADAMS RP, 1990, BIOCHEM SYST ECOL, V18, P107, DOI 10.1016/0305-1978(90)90044-G; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Blazquez MA, 1998, PLANT J, V13, P685, DOI 10.1046/j.1365-313X.1998.00063.x; Bonini BM, 2000, BIOCHEM J, V350, P261, DOI 10.1042/0264-6021:3500261; Boraston AB, 2004, BIOCHEM J, V382, P769, DOI 10.1042/BJ20040892; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Burroughs AM, 2006, J MOL BIOL, V361, P1003, DOI 10.1016/j.jmb.2006.06.049; CABIB E, 1958, J BIOL CHEM, V231, P259; Cai ZJ, 2009, J BIOSCI BIOENG, V107, P499, DOI 10.1016/j.jbiosc.2009.01.007; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2007, NEW PHYTOL, V176, P45, DOI 10.1111/j.1469-8137.2007.02152.x; Collet JF, 1998, J BIOL CHEM, V273, P14107, DOI 10.1074/jbc.273.23.14107; CROWE JH, 1984, SCIENCE, V223, P701, DOI 10.1126/science.223.4637.701; Deng YY, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0039704; DEVIRGILIO C, 1993, EUR J BIOCHEM, V212, P315; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Eastmond PJ, 2003, J EXP BOT, V54, P533, DOI 10.1093/jxb/erg039; Eastmond PJ, 2002, PLANT J, V29, P225, DOI 10.1046/j.1365-313x.2002.01220.x; Elbein A D, 1974, Adv Carbohydr Chem Biochem, V30, P227, DOI 10.1016/S0065-2318(08)60266-8; Elbein AD, 2003, GLYCOBIOLOGY, V13, p17R, DOI 10.1093/glycob/cwg047; Fieulaine S, 2005, PLANT CELL, V17, P2049, DOI 10.1105/tpc.105.031229; Gasteiger E, 2005, PROTEOMICS PROTOCOLS; Geourjon C, 1995, COMPUT APPL BIOSCI, V11, P681; GIAEVER HM, 1988, J BACTERIOL, V170, P2841, DOI 10.1128/jb.170.6.2841-2849.1988; Goddijn OJM, 1999, TRENDS PLANT SCI, V4, P315, DOI 10.1016/S1360-1385(99)01446-6; Iordachescu M, 2008, J INTEGR PLANT BIOL, V50, P1223, DOI 10.1111/j.1744-7909.2008.00736.x; Kosmas SA, 2006, PLANTA, V223, P329, DOI 10.1007/s00425-005-0071-5; Krogh A, 2001, J MOL BIOL, V305, P567, DOI 10.1006/jmbi.2000.4315; Leyman B, 2001, TRENDS PLANT SCI, V6, P510, DOI 10.1016/S1360-1385(01)02125-2; Lunn JE, 2007, FUNCT PLANT BIOL, V34, P550, DOI 10.1071/FP06315; Noubhani A, 2000, EUR J BIOCHEM, V267, P4566, DOI 10.1046/j.1432-1327.2000.01511.x; Penninga D, 1996, J BIOL CHEM, V271, P32777, DOI 10.1074/jbc.271.51.32777; Petersen TN, 2011, NAT METHODS, V8, P785, DOI 10.1038/nmeth.1701; Schmittgen TD, 2000, ANAL BIOCHEM, V285, P194, DOI 10.1006/abio.2000.4753; Sorimachi K, 1997, STRUCTURE, V5, P647, DOI 10.1016/S0969-2126(97)00220-7; Steneck RS, 2002, ENVIRON CONSERV, V29, P436, DOI 10.1017/S0376892902000322; STROM AR, 1993, MOL MICROBIOL, V8, P205, DOI 10.1111/j.1365-2958.1993.tb01564.x; THEVELEIN JM, 1995, TRENDS BIOCHEM SCI, V20, P3, DOI 10.1016/S0968-0004(00)88938-0; Vandesteene L, 2010, MOL PLANT, V3, P406, DOI 10.1093/mp/ssp114; Wang GL, 2010, MAR DRUGS, V8, P2065, DOI 10.3390/md8072065; Xuan JS, 2012, ACTA OCEANOL SIN, V31, P139, DOI 10.1007/s13131-012-0260-6; Yao JT, 2009, J APPL PHYCOL, V21, P233, DOI 10.1007/s10811-008-9354-0; Zentella R, 1999, PLANT PHYSIOL, V119, P1473, DOI 10.1104/pp.119.4.1473	44	9	13	2	22	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0301-4851	1573-4978		MOL BIOL REP	Mol. Biol. Rep.	JAN	2014	41	1					529	536		10.1007/s11033-013-2888-5			8	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	281KP	WOS:000329100500057	24293128				2021-04-07	
J	Tsirigoti, A; Kupper, FC; Gachon, CMM; Katsaros, C				Tsirigoti, A.; Kuepper, F. C.; Gachon, C. M. M.; Katsaros, C.			Cytoskeleton organisation during the infection of three brown algal species, Ectocarpus siliculosus, Ectocarpus crouaniorum and Pylaiella littoralis, by the intracellular marine oomycete Eurychasma dicksonii	PLANT BIOLOGY			English	Article						Actin; brown algae; cytoskeleton; host; infection; microtubule; oomycete	MICROTUBULE ORGANIZATION; BIOTIC INTERACTIONS; LIFE-CYCLE; CELL-CYCLE; PHYTOPHTHORA; ACTIN; PHAEOPHYCEAE; IMMUNOFLUORESCENCE; ZOOSPOROGENESIS; ULTRASTRUCTURE	Oomycete diseases in seaweeds are probably widespread and of significant ecological and economic impact, but overall still poorly understood. This study investigates the organisation of the cytoskeleton during infection of three brown algal species, Pylaiella littoralis, Ectocarpus siliculosus, and Ectocarpus crouaniorum, by the basal marine oomycete Eurychasma dicksonii. Immunofluorescence staining of tubulin revealed how the development of this intracellular biotrophic pathogen impacts on microtubule (MT) organisation of its algal host. The host MT cytoskeleton remains normal and organised by the centrosome until very late stages of the infection. Additionally, the organisation of the parasite's cytoskeleton was examined. During mitosis of the E.dicksonii nucleus the MT focal point (microtubule organisation centre, MTOC, putative centrosome) duplicates and each daughter MTOC migrates to opposite poles of the nucleus. This similarity in MT organisation between the host and pathogen reflects the relatively close phylogenetic relationship between oomycetes and brown algae. Moreover, actin labelling with rhodamine-phalloidin in E.dicksonii revealed typical images of actin dots connected by fine actin filament bundles in the cortical cytoplasm. The functional and phylogenetic implications of our observations are discussed.	[Tsirigoti, A.; Katsaros, C.] Univ Athens, Fac Biol, Dept Bot, Athens 15784, Greece; [Kuepper, F. C.] Univ Aberdeen, Oceanlab, Newburgh, Aberdeen, Scotland; [Gachon, C. M. M.] SAMS, CCAP, Oban, Argyll, Scotland	Katsaros, C (corresponding author), Univ Athens, Fac Biol, Dept Bot, Athens 15784, Greece.	christos.katsaros@biol.uoa.gr		TSIRIGOTI, AMERSSA/0000-0002-5018-3461; Kuepper, Frithjof/0000-0003-1273-7109; Gachon, Claire/0000-0002-3702-7472	TOTAL Foundation; European CommissionEuropean CommissionEuropean Commission Joint Research Centre [227788]; MASTS pooling initiative; Scottish Funding Council [HR09011]; UK Natural Environment Research Council (NERC)UK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/J00460X/1]; NERC - National Capability funding for the Culture Collection of Algae and Protozoa (CCAP); Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [dml010007, NE/D521522/1, NE/F012705/1, NE/J00460X/1] Funding Source: researchfish	The present study was supported by the TOTAL Foundation (Project "Brown algal biodiversity and ecology in the Eastern Mediterranean Sea") and the University of Athens. We gratefully acknowledge funding from the European Commission through the FP7 "capacities' specific programme, ASSEMBLE (grant no. 227788) for both AT and CK for their research visits to the Scottish Association for Marine Science (SAMS, Oban). The work of FCK received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant no.HR09011) and contributing institutions. C.M.M.G. was supported by a New Investigator grant from the UK Natural Environment Research Council (NERC, grant NE/J00460X/1) and NERC - National Capability funding for the Culture Collection of Algae and Protozoa (CCAP).	Beakes GW, 1998, FUNGAL GENET BIOL, V24, P45, DOI 10.1006/fgbi.1998.1072; Bidle KD, 2011, CURR OPIN MICROBIOL, V14, P449, DOI 10.1016/j.mib.2011.07.013; BROWN RC, 1988, CELL MOTIL CYTOSKEL, V11, P139, DOI 10.1002/cm.970110207; BROWN RC, 1994, PLANT CELL, V6, P1241, DOI 10.1105/tpc.6.9.1241; Cahill D, 2002, PLANT PATHOL, V51, P629, DOI 10.1046/j.0032-0862.2002.00758.x; CERENIUS L, 1984, NORD J BOT, V4, P697, DOI 10.1111/j.1756-1051.1984.tb01995.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Czymmek KJ, 1996, J MICROSC-OXFORD, V181, P153, DOI 10.1046/j.1365-2818.1996.108388.x; Duarte CM, 2009, BIOSCIENCE, V59, P967, DOI 10.1525/bio.2009.59.11.8; Gachon CMM, 2007, GENE, V406, P51, DOI 10.1016/j.gene.2007.05.018; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Gay JL, 1966, COLSTON PAP, P95; GOTELLI D, 1974, MYCOLOGIA, V66, P846, DOI 10.2307/3758204; Grenville-Briggs L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0024500; GROSS P, 1993, EMBO J, V12, P1735, DOI 10.1002/j.1460-2075.1993.tb05821.x; Hardham AR, 2007, CURR OPIN PLANT BIOL, V10, P342, DOI 10.1016/j.pbi.2007.05.001; Hardham AR, 1997, ADV BOT RES, V24, P353; Hardham AR, 2009, OOMYCETE GENETICS GE, P93; HEATH IB, 1980, J CELL BIOL, V84, P531, DOI 10.1083/jcb.84.3.531; HEATH IB, 1971, Z ZELLFORSCH MIK ANA, V112, P371; HEATH IB, 1992, J CELL SCI, V102, P611; Heesch S, 2012, CRYOLETTERS, V33, P327; HOLLOWAY SA, 1977, EXP MYCOL, V1, P9, DOI 10.1016/S0147-5975(77)80027-3; HYDE GJ, 1991, J CELL SCI, V100, P735; HYDE GJ, 1991, MYCOL RES, V95, P577, DOI 10.1016/S0953-7562(09)80072-5; HYDE GJ, 1992, EXP MYCOL, V16, P207, DOI 10.1016/0147-5975(92)90029-Q; Jackson SL, 1998, FUNGAL GENET BIOL, V24, P24, DOI 10.1006/fgbi.1998.1071; JELKE E, 1987, J BASIC MICROB, V27, P11, DOI 10.1002/jobm.3620270103; Karyophyllis D, 2000, EUR J PHYCOL, V35, P25, DOI 10.1017/S0967026200002602; KATSAROS C, 1990, BRIT PHYCOL J, V25, P63, DOI 10.1080/00071619000650061; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; KATSAROS C, 1991, BOT ACTA, V104, P87, DOI 10.1111/j.1438-8677.1991.tb00201.x; KATSAROS C, 1992, PROTOPLASMA, V169, P75, DOI 10.1007/BF01343372; KATSAROS C, 1980, THESIS U ATHENS ATHE; Katsaros C, 2013, MAR FRESHW BOTANY, P143; Katsaros Christos I., 1995, Phycological Research, V43, P43, DOI 10.1111/j.1440-1835.1995.tb00004.x; Katsaros CI, 2011, PROTIST, V162, P315, DOI 10.1016/j.protis.2010.10.004; Kobayashi I, 1997, AUST J PLANT PHYSIOL, V24, P733, DOI 10.1071/PP97060; Kobayashi Issei, 2008, V11, P121, DOI 10.1007/7089_2007_144; Kobayashi Y, 1997, PLANT J, V11, P525, DOI 10.1046/j.1365-313X.1997.11030525.x; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Lowry DS, 1997, PROTOPLASMA, V196, P45, DOI 10.1007/BF01281057; MARTIN RW, 1986, MYCOLOGIA, V78, P11, DOI 10.2307/3793371; MENZEL D, 1986, PROTOPLASMA, V134, P30, DOI 10.1007/BF01276373; Muller DG, 2008, CAH BIOL MAR, V49, P59; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; PUESCHEL CM, 1985, CAN J BOT, V63, P409, DOI 10.1139/b85-049; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Singh A, 2011, BIORESOURCE TECHNOL, V102, P10, DOI 10.1016/j.biortech.2010.06.032; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Strittmatter M, 2009, OOMYCETE GENETICS GE, P25; Suttle CA, 2005, NATURE, V437, P356, DOI 10.1038/nature04160; Takemoto D, 2004, PLANT PHYSIOL, V136, P3864, DOI 10.1104/pp.104.052159; Takemoto D, 2003, PLANT J, V33, P775, DOI 10.1046/j.1365-313X.2003.01673.x; Walker SK, 2006, FUNGAL GENET BIOL, V43, P357, DOI 10.1016/j.fgb.2006.01.004; WICK SM, 1985, CELL BIOL INT REP, V9, P357, DOI 10.1016/0309-1651(85)90031-1	62	5	5	1	24	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	1435-8603	1438-8677		PLANT BIOLOGY	Plant Biol.	JAN	2014	16	1					272	281		10.1111/plb.12041			10	Plant Sciences	Plant Sciences	277NS	WOS:000328826900032	23692049	Green Accepted			2021-04-07	
J	Lipinska, AP; D'hondt, S; Van Damme, EJM; De Clerck, O				Lipinska, Agnieszka P.; D'hondt, Sofie; Van Damme, Els J. M.; De Clerck, Olivier			Uncovering the genetic basis for early isogamete differentiation: a case study of Ectocarpus siliculosus	BMC GENOMICS			English	Article						Gamete; Transcriptome; Ectocarpus; Sexual reproduction; Isogamy; Brown alga; Signaling	SEXUAL REPRODUCTION GENE; MATERNAL MESSENGER-RNA; ALGA ECTOCARPUS; BROWN-ALGAE; SIGNAL-TRANSDUCTION; EXPRESSION ANALYSIS; LIFE-CYCLE; G-PROTEIN; RAB PROTEINS; FERTILIZATION	Background: The phenomenon of sexual reproduction characterizes nearly all eukaryotes, with anisogamy being the most prevalent form of gamete discrimination. Since dimorphic gametes most likely descend from equal-sized specialized germ cells, identifying the genetic bases of the early functional diversification in isogametes can provide better understanding of the evolution of sexual dimorphism. However, despite the potential importance to the evolutionary biology field, no comprehensive survey of the transcriptome profiling in isomorphic gametes has been reported hitherto. Results: Gamete differentiation on the genomic level was investigated using Ectocarpus siliculosus, a model organism for brown algal lineage which displays an isogamous sexual reproduction cycle. Transcriptome libraries of male and female gametes were generated using Next Generation Sequencing technology (SOLiD) and analyzed to identify differentially regulated genes and pathways with potential roles in fertilization and gamete specialization. Gamete transcriptomes showed a high level of complexity with a large portion of gender specific gene expression. Our results indicate that over 4,000 of expressed genes are differentially regulated between male and female, including sequences related to cell movement, carbohydrate and lipid metabolism, signaling, transport and RNA processing. Conclusions: This first comprehensive transcriptomic study of protist isogametes describes considerable adaptation to distinct sexual roles, suggesting that functional anisogamy precedes morphological differentiation. Several sex-biased genes and pathways with a putative role in reproduction were identified, providing the basis for more detailed investigations of the mechanisms underlying evolution of mating types and sexual dimorphism.	[Lipinska, Agnieszka P.; D'hondt, Sofie; De Clerck, Olivier] Univ Ghent, Phycol Res Grp, B-9000 Ghent, Belgium; [Lipinska, Agnieszka P.; D'hondt, Sofie; De Clerck, Olivier] Univ Ghent, Ctr Mol Phylogenet & Evolut, B-9000 Ghent, Belgium; [Van Damme, Els J. M.] Univ Ghent, Dept Mol Biotechnol, Lab Biochem & Glycobiol, B-9000 Ghent, Belgium	Lipinska, AP (corresponding author), Univ Ghent, Phycol Res Grp, Krijgslaan 281,Bldg S8, B-9000 Ghent, Belgium.	ap.lipinska@gmail.com	De Clerck, Olivier/ABC-2683-2020; De Clerck, Olivier/AAU-4295-2020; Van Damme, Els J/B-4410-2015; Van Damme, Els/U-3904-2019; De Clerck, Olivier/A-9083-2010	De Clerck, Olivier/0000-0002-3699-8402; Van Damme, Els/0000-0001-9848-766X; De Clerck, Olivier/0000-0002-3699-8402	BOF, Ghent University, BelgiumGhent University [09/24J/117]	This work was funded by BOF Grant 09/24J/117, Ghent University, Belgium. We gratefully acknowledge Dieter Muller for providing the Ectocarpus strain used in this study and for helpful instructions on culturing. We thank Erwan Corre for guidance and advice in setting up the bioinformatic analysis as well as Susana Coelho and Mark Cock for helpful discussions and words of encouragement.	Alves AP, 1997, J BIOL CHEM, V272, P6965, DOI 10.1074/jbc.272.11.6965; Arab K, 2006, BRIT J NUTR, V96, P811, DOI 10.1017/BJN20061910; Armbrust EV, 1999, APPL ENVIRON MICROB, V65, P3121; Armbrust EV, 2001, APPL ENVIRON MICROB, V67, P3501, DOI 10.1128/AEM.67.8.3501-3513.2001; BABCOCK DF, 1992, P NATL ACAD SCI USA, V89, P6001, DOI 10.1073/pnas.89.13.6001; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Baron M, 2003, SEMIN CELL DEV BIOL, V14, P113, DOI 10.1016/S1084-9521(02)00179-9; BELL G, 1978, J THEOR BIOL, V73, P247, DOI 10.1016/0022-5193(78)90189-3; Bell G, 1997, BIOL J LINN SOC, V60, P21, DOI 10.1111/j.1095-8312.1997.tb01481.x; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; Berman SA, 2003, CURR BIOL, V13, P1145, DOI 10.1016/S0960-9822(03)00415-9; Bisgrove SR, 2007, J INTEGR PLANT BIOL, V49, P1192, DOI 10.1111/j.1672-9072.2007.00518.x; Bockaert J, 1999, EMBO J, V18, P1723, DOI 10.1093/emboj/18.7.1723; BOLWELL GP, 1979, J CELL SCI, V36, P19; Borges F, 2008, PLANT PHYSIOL, V148, P1168, DOI 10.1104/pp.108.125229; BOUCK GB, 1971, J CELL BIOL, V50, P362, DOI 10.1083/jcb.50.2.362; BRAWLEY SH, 1992, MAR BIOL, V113, P145, DOI 10.1007/BF00367648; BREDEROO J, 1991, PLANTA, V184, P175, DOI 10.1007/BF00197945; Brownlee C, 2001, SEMIN CELL DEV BIOL, V12, P345, DOI 10.1006/scdb.2001.0262; Burglin TR, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-127; CALLOW ME, 1978, J CELL SCI, V32, P337; CALLOW ME, 1978, J CELL SCI, V32, P45; Carroll DJ, 1997, J CELL BIOL, V138, P1303, DOI 10.1083/jcb.138.6.1303; Cavalier-Smith T, 2002, HEREDITY, V88, P125, DOI 10.1038/sj.hdy.6800034; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2012, ADV BOT RES, V64, P141, DOI 10.1016/B978-0-12-391499-6.00005-0; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2002, PLANT CELL, V14, P2369, DOI 10.1105/tpc.003285; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Conesa A, 2005, BIOINFORMATICS, V21, P3674, DOI 10.1093/bioinformatics/bti610; Dadoune JP, 2004, INT REV CYTOL, V237, P1, DOI 10.1016/S0074-7696(04)37001-4; Darszon A, 2008, INT J DEV BIOL, V52, P595, DOI 10.1387/ijdb.072550ad; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dixon NM, 2000, PHYCOLOGIA, V39, P258, DOI 10.2216/i0031-8884-39-3-258.1; Ehebauer M, 2006, SCIENCE, V314, P1414, DOI 10.1126/science.1134042; Engel ML, 2003, PLANT J, V34, P697, DOI 10.1046/j.1365-313X.2003.01761.x; Evsikov AV, 2006, GENE DEV, V20, P2713, DOI 10.1101/gad.1471006; Ferris P, 2010, SCIENCE, V328, P351, DOI 10.1126/science.1186222; Ferris PJ, 1997, GENETICS, V146, P859; Galindo BE, 2000, DEV BIOL, V221, P285, DOI 10.1006/dbio.2000.9678; Gazave E, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-249; Gillard J, 2009, THESIS GHENT U GHENT; Goddard H, 2000, P NATL ACAD SCI USA, V97, P1932, DOI 10.1073/pnas.020516397; Granados-Gonzalez G, 2005, FEBS LETT, V579, P6667, DOI 10.1016/j.febslet.2005.10.035; Gunaratne HJ, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-235; Halet G, 2002, J CELL SCI, V115, P2139; HANSBROUGH JR, 1980, BIOCHIM BIOPHYS ACTA, V630, P82, DOI 10.1016/0304-4165(80)90139-7; Hellemans J, 2007, GENOME BIOL, V8, DOI 10.1186/gb-2007-8-2-r19; Hoekstra R F, 1987, Experientia Suppl, V55, P59; Honda D, 2007, PROTIST, V158, P77, DOI 10.1016/j.protis.2006.08.004; Kasahara RD, 2005, PLANT CELL, V17, P2981, DOI 10.1105/tpc.105.034603; Khurana JS, 2010, J CELL BIOL, V191, P905, DOI 10.1083/jcb.201006034; Kirchhausen T, 2000, NAT REV MOL CELL BIO, V1, P187, DOI 10.1038/35043117; Kirk DL, 2006, CURR BIOL, V16, pR1028, DOI 10.1016/j.cub.2006.11.015; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Krawetz SA, 2005, NAT REV GENET, V6, P633, DOI 10.1038/nrg1654; Kumakiri J, 2003, DEV BIOL, V260, P522, DOI 10.1016/S0012-1606(03)00273-2; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; LEE HC, 1986, J BIOL CHEM, V261, P6026; Lee SJ, 1998, DEV BIOL, V193, P195, DOI 10.1006/dbio.1997.8792; Loewith R, 2011, GENETICS, V189, P1177, DOI 10.1534/genetics.111.133363; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; Maier I, 1997, EUR J PHYCOL, V32, P255; Rojas AM, 2012, J CELL BIOL, V196, P189, DOI 10.1083/jcb.201103008; Martins MJF, 2013, BMC GENOMICS, V14, DOI 10.1186/1471-2164-14-294; MASTERS AK, 1992, PLANT J, V2, P619, DOI 10.1111/j.1365-313X.1992.00619.x; Maynard-Smith J., 1978, EVOLUTION SEX; McDougall A, 2000, BIOL CELL, V92, P205, DOI 10.1016/S0248-4900(00)01073-X; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Mourao PAS, 2007, BRAZ J MED BIOL RES, V40, P5, DOI 10.1590/S0100-879X2007000100002; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; Muller DG, 2000, BOT MAR, V43, P157, DOI 10.1515/BOT.2000.016; Neill AT, 2004, REPRODUCTION, V127, P141, DOI 10.1530/rep.1.00085; Neves SR, 2002, SCIENCE, V296, P1636, DOI 10.1126/science.1071550; Novick P, 1997, CURR OPIN CELL BIOL, V9, P496, DOI 10.1016/S0955-0674(97)80025-7; Ohnishi T, 2011, PLANT PHYSIOL, V155, P881, DOI 10.1104/pp.110.167502; Ohta K, 2000, GLYCOCONJUGATE J, V17, P205, DOI 10.1023/A:1026589223811; Okada T, 2007, PLANT CELL REP, V26, P1045, DOI 10.1007/s00299-006-0300-9; Pedersen LB, 2008, CURR TOP DEV BIOL, V85, P23, DOI 10.1016/S0070-2153(08)00802-8; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Pohnert G, 2002, NAT PROD REP, V19, P108, DOI 10.1039/a806888g; Punwani JA, 2007, PLANT CELL, V19, P2557, DOI 10.1105/tpc.107.052076; Ramesh MA, 2005, CURR BIOL, V15, P185, DOI 10.1016/j.cub.2005.01.003; Rayko E, 2010, NEW PHYTOL, V188, P52, DOI 10.1111/j.1469-8137.2010.03371.x; Robinson MD, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-3-r25; Robinson MD, 2010, BIOINFORMATICS, V26, P139, DOI 10.1093/bioinformatics/btp616; Rozen S, 2000, Methods Mol Biol, V132, P365; Rumble SM, 2009, PLOS COMPUT BIOL, V5, DOI 10.1371/journal.pcbi.1000386; Ruthmann A., 1970, METHODS CELL RES; SCHMID CE, 1994, J PLANT PHYSIOL, V143, P570, DOI 10.1016/S0176-1617(11)81826-2; SCHMID CE, 1993, HYDROBIOLOGIA, V261, P437; Schmidt A, 1997, CELL, V88, P531, DOI 10.1016/S0092-8674(00)81893-0; Schmitz AAP, 2000, EXP CELL RES, V261, P1, DOI 10.1006/excr.2000.5049; Schnaar RL, 2009, ESSENTIALS GLYCOBIOL; Schoenwaelder MEA, 2000, PLANT BIOLOGY, V2, P24, DOI 10.1055/s-2000-9178; Senger T, 2005, J BIOL CHEM, V280, P7588, DOI 10.1074/jbc.M411738200; Sheehan D, 2001, BIOCHEM J, V360, P1, DOI 10.1042/0264-6021:3600001; Shiba K, 2008, P NATL ACAD SCI USA, V105, P19312, DOI 10.1073/pnas.0808580105; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; SILLERS PJ, 1985, CELL BIOL INT REP, V9, P275, DOI 10.1016/0309-1651(85)90044-X; SOMMERVILLE J, 1990, J REPROD FERTIL, P225; Sorhannus U, 2006, J MOL EVOL, V63, P231, DOI 10.1007/s00239-006-0016-z; Spehr M, 2004, J BIOL CHEM, V279, P40194, DOI 10.1074/jbc.M403913200; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Stearns SC, 1987, EVOLUTION SEX ITS CO; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; Strunker T, 2006, NAT CELL BIOL, V8, P1149, DOI 10.1038/ncb1473; Townley IK, 2006, SEMIN CELL DEV BIOL, V17, P293, DOI 10.1016/j.semcdb.2006.02.006; Trapnell C, 2009, BIOINFORMATICS, V25, P1105, DOI 10.1093/bioinformatics/btp120; Umen JG, 2011, CURR OPIN MICROBIOL, V14, P634, DOI 10.1016/j.mib.2011.10.005; Vallee M, 2008, REPRODUCTION, V135, P439, DOI 10.1530/REP-07-0342; Vandesompele J, 2002, GENOME BIOL, V3, DOI 10.1186/gb-2002-3-7-research0034; Wang Q, 2006, CELL, V125, P549, DOI 10.1016/j.cell.2006.02.044; WARD CR, 1992, J BIOL CHEM, V267, P14061; Werner T, 2010, BRIEF BIOINFORM, V11, P499, DOI 10.1093/bib/bbq018; West JA, 1999, HYDROBIOLOGIA, V399, P101; Wettschureck N, 2005, PHYSIOL REV, V85, P1159, DOI 10.1152/physrev.00003.2005; Wilding M, 1997, MOL HUM REPROD, V3, P269, DOI 10.1093/molehr/3.3.269; Wu JM, 2006, NUCLEIC ACIDS RES, V34, pW720, DOI 10.1093/nar/gkl167; Wuest SE, 2010, CURR BIOL, V20, P506, DOI 10.1016/j.cub.2010.01.051; Xin HP, 2011, SEX PLANT REPROD, V24, P37, DOI 10.1007/s00497-010-0151-y; Yamagishi T, 2009, J PHYCOL, V45, P1110, DOI 10.1111/j.1529-8817.2009.00722.x; Zambounis A, 2012, MOL BIOL EVOL, V29, P1263, DOI 10.1093/molbev/msr296; Zerial M, 2001, NAT REV MOL CELL BIO, V2, P107, DOI 10.1038/35052055	128	17	17	0	39	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2164			BMC GENOMICS	BMC Genomics	DEC 21	2013	14								909	10.1186/1471-2164-14-909			15	Biotechnology & Applied Microbiology; Genetics & Heredity	Biotechnology & Applied Microbiology; Genetics & Heredity	285BG	WOS:000329367000003	24359479	DOAJ Gold, Green Published			2021-04-07	
J	Green, JJ; Cervantes, DC; Peters, NT; Logan, KO; Kropf, DL				Green, Jeffrey J.; Cervantes, Diego Cordero; Peters, Nick T.; Logan, Kyle O.; Kropf, Darryl L.			Dynamic microtubules and endomembrane cycling contribute to polarity establishment and early development of Ectocarpus mitospores	PROTOPLASMA			English	Article						Brown algae; Cell polarity; Cytoskeleton; Ectocarpus; Photopolarization; Secretion	ENDOPLASMIC-RETICULUM; DINITROANILINE HERBICIDE; BREFELDIN-A; CELL-CYCLE; PHAEOPHYCEAE; TUBULIN; CENTRIN; GROWTH; IMMUNOLOCALIZATION; IMMUNOFLUORESCENCE	Many zygotes and spores of brown algae are photosensitive and establish a developmental axis in accordance with directional light cues. Ectocarpus siliculosus is being advanced as a genetic and genomic model organism for investigating brown alga development, and this report investigates photopolarization of the growth axis of mitospores. When exposed to unidirectional light, mitospores photopolarized and established a growth axis such that germination was preferentially localized to the shaded hemisphere of the spore body. The roles of the microtubule cytoskeleton and endomembrane cycling in the photopolarization process were investigated using pharmacological agents. Disruption of microtubule dynamics progressively reduced the percentage of mitospores that photopolarized, while inhibition of vesicle secretion blocked photopolarization nearly completely. Chronic treatment with these pharmacological agents severely affected algal morphogenesis. Microtubules in mitospores and algal filaments were imaged by confocal microscopy. Mitospores contained a radial microtubule array, emanating from a centrosome associated with the nuclear envelope. At germination, the radial array gradually transitioned into a longitudinal array with microtubules extending into the emerging apex. At mitosis, spindles were aligned with the growth axis of cylindrical cells in the filament, and the division plane bisected the spindle axis. These studies demonstrate that dynamic membrane cycling and microtubule assembly play fundamental roles in photopolarization and provide a foundation for future genetic and genomic investigations of this important developmental process.	[Green, Jeffrey J.; Cervantes, Diego Cordero; Peters, Nick T.; Logan, Kyle O.; Kropf, Darryl L.] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA	Kropf, DL (corresponding author), Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA.	kropf@bioscience.utah.edu		Green, Jeffrey/0000-0003-1363-7775	National Science FoundationNational Science Foundation (NSF) [IOS 0817045]; University of Utah	We thank Dr. Ed King for his help with confocal imaging and for engaging discussions and Professor Whitney Hable for valuable comments on the manuscript. This research was supported by the National Science Foundation award IOS 0817045 and a Seed Grant from the University of Utah.	Alessa L, 1999, DEVELOPMENT, V126, P201; Atilgan E, 2012, CYTOSKELETON, V69, P973, DOI 10.1002/cm.21068; Barr FA, 2002, CURR OPIN CELL BIOL, V14, P496, DOI 10.1016/S0955-0674(02)00345-9; BENTRUP FW, 1968, PROTOPLASMA, V65, P25, DOI 10.1007/BF01666369; Bibikova TN, 1999, PLANT J, V17, P657, DOI 10.1046/j.1365-313X.1999.00415.x; Bisgrove SR, 1997, J PHYCOL, V33, P823, DOI 10.1111/j.0022-3646.1997.00823.x; Campas O, 2009, CURR BIOL, V19, P2102, DOI 10.1016/j.cub.2009.10.075; Chang F, 2009, CSH PERSPECT BIOL, V1, DOI 10.1101/cshperspect.a001347; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P369, DOI 10.1101/pdb.prot067975; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P258, DOI 10.1101/pdb.prot067934; Coelho Susana M, 2012, Cold Spring Harb Protoc, V2012, P193, DOI 10.1101/pdb.emo065821; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Du YR, 2004, J CELL SCI, V117, P2871, DOI 10.1242/jcs.01286; Feierbach B, 2004, J CELL BIOL, V165, P697, DOI 10.1083/jcb.200403090; Hable WE, 2000, DEVELOPMENT, V127, P493; Hable WE, 1998, DEV BIOL, V198, P45, DOI 10.1016/S0012-1606(98)80028-6; Hadley R, 2006, BMC PLANT BIOL, V6, DOI 10.1186/1471-2229-6-5; HUGDAHL JD, 1993, PLANT PHYSIOL, V102, P725, DOI 10.1104/pp.102.3.725; Karyophyllis D, 2000, EUR J PHYCOL, V35, P25, DOI 10.1017/S0967026200002602; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; KATSAROS C, 1991, BOT ACTA, V104, P87, DOI 10.1111/j.1438-8677.1991.tb00201.x; Katsaros C, 1997, PHYCOLOGIA, V36, P60, DOI 10.2216/i0031-8884-36-1-60.1; Katsaros Christos I., 1995, Phycological Research, V43, P43, DOI 10.1111/j.1440-1835.1995.tb00004.x; KATSAROS CI, 1992, BOT ACTA, V105, P400, DOI 10.1111/j.1438-8677.1992.tb00320.x; KATSAROS CI, 1993, J PHYCOL, V29, P787, DOI 10.1111/j.0022-3646.1993.00787.x; Kropf DL, 1999, TRENDS PLANT SCI, V4, P490, DOI 10.1016/S1360-1385(99)01509-5; Kropf DL, 1998, CURR OPIN CELL BIOL, V10, P117, DOI 10.1016/S0955-0674(98)80094-X; KROPF DL, 1990, J CELL SCI, V97, P545; Lane JD, 1999, MOL BIOL CELL, V10, P1909, DOI 10.1091/mbc.10.6.1909; MOREJOHN LC, 1987, PLANTA, V172, P252, DOI 10.1007/BF00394595; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters NT, 2006, BMC PLANT BIOL, V6, DOI 10.1186/1471-2229-6-19; Peters NT, 2010, CYTOSKELETON, V67, P102, DOI 10.1002/cm.20427; Phillips N, 2008, J PHYCOL, V44, P15, DOI 10.1111/j.1529-8817.2007.00435.x; QUATRANO RS, 1973, DEV BIOL, V30, P209, DOI 10.1016/0012-1606(73)90059-6; Ritzenthaler C, 2002, PLANT CELL, V14, P237, DOI 10.1105/tpc.010237; Sawin KE, 2004, J CELL SCI, V117, P689, DOI 10.1242/jcs.00925; Sieberer BJ, 2005, NEW PHYTOL, V167, P711, DOI 10.1111/j.1469-8137.2005.01506.x; Siegrist SE, 2007, GENE DEV, V21, P483, DOI 10.1101/gad.1511207; Takahashi F, 2007, P NATL ACAD SCI USA, V104, P19625, DOI 10.1073/pnas.0707692104; Waterman-Storer CM, 1998, CURR BIOL, V8, P798, DOI 10.1016/S0960-9822(98)70321-5; Zeeh JC, 2006, J BIOL CHEM, V281, P11805, DOI 10.1074/jbc.M600149200	44	1	1	0	7	SPRINGER WIEN	WIEN	SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA	0033-183X	1615-6102		PROTOPLASMA	Protoplasma	OCT	2013	250	5					1035	1043		10.1007/s00709-012-0476-5			9	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	227RY	WOS:000325132800007	23322087				2021-04-07	
J	Kroth, PG				Kroth, Peter G.			GETTING A GRIP ON GENETIC MODIFICATION IN BROWN ALGAE	JOURNAL OF PHYCOLOGY			English	Article							DIATOM PHAEODACTYLUM-TRICORNUTUM; CHLOROPLAST TRANSFORMATION; CHLAMYDOMONAS-REINHARDTII; NUCLEAR TRANSFORMATION; LIFE-CYCLE; ECTOCARPUS; GENOME; PROSPECTS; CELLS; YEAST		Univ Konstanz, Dept Biol, D-78457 Constance, Germany	Kroth, PG (corresponding author), Univ Konstanz, Dept Biol, D-78457 Constance, Germany.	Peter.Kroth@uni-konstanz.de	Kroth, Peter G/A-9728-2008	Kroth, Peter G/0000-0003-4734-8955			Apt KE, 1996, MOL GEN GENET, V252, P572, DOI 10.1007/s004380050264; Beer LL, 2009, CURR OPIN BIOTECH, V20, P264, DOI 10.1016/j.copbio.2009.06.002; Bent AF, 2000, PLANT PHYSIOL, V124, P1540, DOI 10.1104/pp.124.4.1540; BROWNLEE C, 1986, NATURE, V320, P624, DOI 10.1038/320624a0; Cermak T, 2011, NUCLEIC ACIDS RES, V39, DOI 10.1093/nar/gkr218; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Cong L, 2013, SCIENCE, V339, P819, DOI 10.1126/science.1231143; Corellou F, 2005, J CELL SCI, V118, P2723, DOI 10.1242/jcs.02353; Davis BM, 1903, ANN BOT-LONDON, V17, P477, DOI 10.1093/oxfordjournals.aob.a088928; Doetsch NA, 2001, CURR GENET, V39, P49, DOI 10.1007/s002940000174; Dunahay TG, 1995, J PHYCOL, V31, P1004, DOI 10.1111/j.0022-3646.1995.01004.x; Epinat JC, 2003, NUCLEIC ACIDS RES, V31, P2952, DOI 10.1093/nar/gkg375; Falciatore A, 1999, MAR BIOTECHNOL, V1, P239, DOI 10.1007/PL00011773; Farnham G., 2013, J PHYCOL, DOI 10. 1111/jpy. 12096; Hammerling Joachim, 1934, Wilhelm Roux Arch Entwickl Mech Org, V131, P1, DOI 10.1007/BF00649809; Harris EH, 2001, ANNU REV PLANT PHYS, V52, P363, DOI 10.1146/annurev.arplant.52.1.363; Materna AC, 2009, J PHYCOL, V45, P838, DOI 10.1111/j.1529-8817.2009.00711.x; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1979, NATURE, V279, P430, DOI 10.1038/279430a0; Purton S, 2013, RUSS J PLANT PHYSL+, V60, P491, DOI 10.1134/S1021443713040146; Qin S, 1994, OCEANOL LIMNOL SIN, V25, P353; Rochaix JD, 1995, ANNU REV GENET, V29, P209, DOI 10.1146/annurev.ge.29.120195.001233; SCHAEFER D, 1991, MOL GEN GENET, V226, P418, DOI 10.1007/BF00260654; SCHIEDLMEIER B, 1994, P NATL ACAD SCI USA, V91, P5080, DOI 10.1073/pnas.91.11.5080; TAKAHASHI Y, 1991, EMBO J, V10, P2033, DOI 10.1002/j.1460-2075.1991.tb07733.x	26	0	0	0	16	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	OCT	2013	49	5					816	818		10.1111/jpy.12110			3	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	264SB	WOS:000327899700002	27007307				2021-04-07	
J	Farnham, G; Strittmatter, M; Coelho, S; Cock, JM; Brownlee, C				Farnham, Garry; Strittmatter, Martina; Coelho, Susana; Cock, Jeremy Mark; Brownlee, Colin			GENE SILENCING IN FUCUS EMBRYOS: DEVELOPMENTAL CONSEQUENCES OF RNAi-MEDIATED CYTOSKELETAL DISRUPTION	JOURNAL OF PHYCOLOGY			English	Article						actin; cytoskeleton; embryo; fucus; RNAi; tubulin	POLARITY ESTABLISHMENT; ASYMMETRIC DIVISION; ECTOCARPUS GENOME; BROWN; POLARIZATION; EMBRYOGENESIS; MICROTUBULES; BIOGENESIS; EVOLUTION; ABUNDANCE	Brown algae (Phaeophyceae) are an important algal class that play a range of key ecological roles. They are often important components of rocky shore communities. A number of members of the Fucales and Ectocarpales have provided models for the study of multicellular evolution, reproductive biology and polarized development. Indeed the fucoid algae exhibit the unusual feature of inducible embryo polarization, allowing many classical studies of polarity induction. The potential of further studies of brown algae in these important areas has been increasingly hindered by the absence of tools for manipulation of gene expression that would facilitate further mechanistic analysis and gene function studies at a molecular level. The aim of this study was to establish a method that would allow the analysis of gene function through RNAi-mediated gene knockdown. We show that injection of double-stranded RNA (dsRNA) corresponding to an -tubulin gene into Fucus serratus Linnaeus zygotes induces the loss of a large proportion of the microtubule cytoskeleton, leading to growth arrest and disruption of cell division. Injection of dsRNA targeting -actin led to reduced rhizoid growth, enlarged cells and the failure to develop apical hair cells. The silencing effect on actin expression was maintained for 3months. These results indicate that the Fucus embryo possesses a functional RNA interference system that can be exploited to investigate gene function during embryogenesis.	[Farnham, Garry; Brownlee, Colin] Marine Biol Assoc UK, Plymouth PL1 2PB, Devon, England; [Strittmatter, Martina; Coelho, Susana; Cock, Jeremy Mark] Univ Paris 06, CNRS, UMR 7139, Marine Plants & Biomol Lab,Stn Biol Roscoff, F-29682 Roscoff, France; [Brownlee, Colin] Univ Southampton, Sch Ocean & Earth Sci, Natl Oceanog Ctr, Southampton SO14 3ZH, Hants, England	Brownlee, C (corresponding author), Marine Biol Assoc UK, Citadel Hill, Plymouth PL1 2PB, Devon, England.	cbr@mba.ac.uk	Coelho, Susana/ABH-8166-2020	Strittmatter, Martina/0000-0002-1258-9751; Cock, J. Mark/0000-0002-2650-0383	Interreg IV Program France (Channel)-England (project Marinexus); Centre National de Recherche Scientifique; University Pierre and Marie Curie; Marine Genomics Europe Network of Excellence; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [REI20579, P18266] Funding Source: researchfish	This work was supported by the Interreg IV Program France (Channel)-England (project Marinexus), the Centre National de Recherche Scientifique, the University Pierre and Marie Curie and the Marine Genomics Europe Network of Excellence.	Arvey A, 2010, MOL SYST BIOL, V6, DOI 10.1038/msb.2010.24; Bisgrove SR, 2007, J INTEGR PLANT BIOL, V49, P1192, DOI 10.1111/j.1672-9072.2007.00518.x; Bisgrove SR, 1998, DEV BIOL, V194, P246, DOI 10.1006/dbio.1997.8832; Bisgrove SR, 2003, PLANT CELL, V15, P854, DOI 10.1105/tpc.009415; Bisgrove SR, 2001, J CELL SCI, V114, P4319; Bothwell JHF, 2008, DEVELOPMENT, V135, P2173, DOI 10.1242/dev.017558; Bouget FY, 1998, DEVELOPMENT, V125, P1999; BRAWLEY SH, 1987, DEV BIOL, V124, P390, DOI 10.1016/0012-1606(87)90491-X; BRAWLEY SH, 1979, J PHYCOL, V15, P266; Brownlee C, 1998, SEMIN CELL DEV BIOL, V9, P179, DOI 10.1006/scdb.1997.0212; Brownlee C, 2001, SEMIN CELL DEV BIOL, V12, P345, DOI 10.1006/scdb.2001.0262; Carthew RW, 2009, CELL, V136, P642, DOI 10.1016/j.cell.2009.01.035; Cerutti H, 2011, EUKARYOT CELL, V10, P1164, DOI 10.1128/EC.05106-11; Chapman EJ, 2007, NAT REV GENET, V8, P884, DOI 10.1038/nrg2179; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Corellou F, 2005, J CELL SCI, V118, P2723, DOI 10.1242/jcs.02353; Dalmay T, 2000, CELL, V101, P543, DOI 10.1016/S0092-8674(00)80864-8; De Riso V, 2009, NUCLEIC ACIDS RES, V37, DOI 10.1093/nar/gkp448; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Fowler JE, 2004, PLANTA, V219, P856, DOI 10.1007/s00425-004-1283-9; GOODNER B, 1993, PLANT CELL, V5, P1471; Hable WE, 2003, PROTOPLASMA, V221, P193, DOI 10.1007/s0070-002-0081-0; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Huang AY, 2011, BMC GENOMICS, V12, DOI 10.1186/1471-2164-12-337; Hugh DJM, 2003, FAO FISHERIES TECHNI, P1; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; Klarzynski O, 2003, MOL PLANT MICROBE IN, V16, P115, DOI 10.1094/MPMI.2003.16.2.115; Lund E, 2011, GENE DEV, V25, P1121, DOI 10.1101/gad.2038811; MASTERS AK, 1992, PLANT J, V2, P619, DOI 10.1111/j.1365-313X.1992.00619.x; Miki H, 2005, TRENDS CELL BIOL, V15, P467, DOI 10.1016/j.tcb.2005.07.006; Motomura T, 2004, HYDROBIOLOGIA, V512, P171, DOI 10.1023/B:HYDR.0000020324.85541.da; Norden-Krichmar TM, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0022870; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters NT, 2006, BMC PLANT BIOL, V6, DOI 10.1186/1471-2229-6-19; Peters NT, 2010, CYTOSKELETON, V67, P102, DOI 10.1002/cm.20427; QUATRANO RS, 1973, DEV BIOL, V30, P209, DOI 10.1016/0012-1606(73)90059-6; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; ROBERTS SK, 1994, DEVELOPMENT, V120, P155; Svoboda P, 2000, DEVELOPMENT, V127, P4147; Takahashi F, 2007, P NATL ACAD SCI USA, V104, P19625, DOI 10.1073/pnas.0707692104; Voinnet O, 2009, CELL, V136, P669, DOI 10.1016/j.cell.2009.01.046; Whisson SC, 2005, MOL PLANT PATHOL, V6, P153, DOI 10.1111/J.1364-3703.2005.00272.X	46	16	16	0	22	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	OCT	2013	49	5					819	829		10.1111/jpy.12096			11	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	264SB	WOS:000327899700003	27007308				2021-04-07	
J	McCarty, AT; Sotka, EE				McCarty, Amanda T.; Sotka, Erik E.			Geographic variation in feeding preference of a generalist herbivore: the importance of seaweed chemical defenses	OECOLOGIA			English	Article						Local adaptation; Feeding preference; Plant chemical defenses; Herbivore offense; Ampithoidae; Phylogeography	HOST USE; LARVAL ADAPTATION; MARINE; AMPHIPODS; POPULATIONS; PERFORMANCE; TEMPERATE; SPECIALIZATION; PALATABILITY; METABOLITES	The ecological impacts of generalist herbivores depend on feeding preferences, which can vary across and within herbivore species. Among mesoherbivores, geographic variation in host use can occur because host plants have a more restricted geographic distribution than does the herbivore, or there is local evolution in host preference, or both. We tested the role of local evolution using the marine amphipod Ampithoe longimana by rearing multiple amphipod populations from three regions (subtropical Florida, warm-temperate North Carolina and cold-temperate New England) and assaying their feeding preferences toward ten seaweeds that occur in some but not all regions. Six of the ten seaweeds produce anti-herbivore secondary metabolites, and we detected geographic variation in feeding preference toward five (Dictyota menstrualis, Dictyota ciliolata, Fucus distichus, Chondrus crispus and Padina gymnospora, but not Caulerpa sertularioides). Amphipod populations that co-occur with a chemically-rich seaweed tended to have stronger feeding preferences for that seaweed, relative to populations that do not co-occur with the seaweed. A direct test indicated that geographic variation in feeding preference toward one seaweed (D. ciliolata) is mediated by feeding tolerance for lipophilic secondary metabolites. Among the four seaweeds that produce no known secondary metabolites (Acanthophora, Ectocarpus, Gracilaria and Hincksia/Feldmannia spp.), we detected no geographic variation in feeding preference. Thus, populations are more likely to evolve greater feeding preferences for local hosts when those hosts produce secondary metabolites. Microevolution of feeding behaviors of generalist marine consumers likely depends on the availability and identity of local hosts and the strength of their chemical defenses.	[McCarty, Amanda T.; Sotka, Erik E.] Coll Charleston, Dept Biol, Charleston, SC 29412 USA; [McCarty, Amanda T.; Sotka, Erik E.] Coll Charleston, Grice Marine Lab, Charleston, SC 29412 USA	Sotka, EE (corresponding author), Coll Charleston, Dept Biol, Charleston, SC 29412 USA.	SotkaE@cofc.edu			National Science FoundationNational Science Foundation (NSF) [OCE- 0550245, DEB-0919064]	We thank Brentley Wiles, Hannah Giddens, Beth Cushman, Carol Thornber, Niels Lindquist, Valerie Paul, and many others for their assistance with field and laboratory work and for logistical help. We thank Lou Burnett, Fran VanDolah, Courtney Murren, Geoff Trussell, and three anonymous reviewers for their thoughtful comments. This work was supported by the National Science Foundation (OCE- 0550245 and DEB-0919064). This is Grice Publication #392.	BELL SS, 1991, ECOLOGY, V72, P350, DOI 10.2307/1938929; BENZ MC, 1979, BOT MAR, V22, P413, DOI 10.1515/botm.1979.22.7.413; Berenbaum MR, 1996, AM NAT, V148, pS139, DOI 10.1086/285907; Bolser RC, 1996, ECOLOGY, V77, P2269, DOI 10.2307/2265730; Bouarab K, 2004, PLANT PHYSIOL, V135, P1838, DOI 10.1104/pp.103.037622; Bousfield EL, 1973, SHALLOW WATER GAMMAR; Brooks RA, 2001, J EXP MAR BIOL ECOL, V264, P67, DOI 10.1016/S0022-0981(01)00310-0; Burkepile DE, 2008, P NATL ACAD SCI USA, V105, P16201, DOI 10.1073/pnas.0801946105; Chavanich S, 2000, CRUSTACEANA, V73, P835, DOI 10.1163/156854000504840; Choat JH, 1998, ANNU REV ECOL SYST, V29, P375, DOI 10.1146/annurev.ecolsys.29.1.375; CRONIN G, 1995, MAR ECOL PROG SER, V119, P265, DOI 10.3354/meps119265; Cronin G, 1996, ECOLOGY, V77, P2287, DOI 10.2307/2265731; Cruz-Rivera E, 2001, MAR ECOL PROG SER, V218, P249, DOI 10.3354/meps218249; Cruz-Rivera E, 2006, HARMFUL ALGAE, V5, P497, DOI 10.1016/j.hal.2005.09.003; Dawes C. J., 1974, MARINE ALGAE W COAST; DAYTON PK, 1985, ECOL MONOGR, V55, P447, DOI 10.2307/2937131; DUFFY JE, 1991, ECOLOGY, V72, P1286, DOI 10.2307/1941102; DUFFY JE, 1994, ECOLOGY, V75, P1304, DOI 10.2307/1937456; Duffy JE, 2000, ECOL MONOGR, V70, P237, DOI 10.1890/0012-9615(2000)070[0237:SIOGAO]2.0.CO;2; Felsenstein J., 1989, CLADISTICS, V5, P164, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X; FOX LR, 1981, SCIENCE, V211, P887, DOI 10.1126/science.211.4485.887; Geiselman JA, 1980, THESIS WOODS HOLE OC; Guindon S, 2010, SYST BIOL, V59, P307, DOI 10.1093/sysbio/syq010; HACKER SD, 1990, ECOLOGY, V71, P2269, DOI 10.2307/1938638; Hay Mark E., 1992, P371; Hay ME, 1997, CORAL REEFS, V16, pS67, DOI 10.1007/s003380050243; HUMM H. J, 1969, PHYCOLOGIA, V7, P43, DOI DOI 10.2216/I0031-8884-7-1-43.1; Kubanek J, 2004, MAR ECOL PROG SER, V277, P79, DOI 10.3354/meps277079; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; LeCroy SE, 2002, ILLUSTRATED IDENTIFI, V2; Levin PS, 2002, MAR ECOL PROG SER, V232, P239, DOI 10.3354/meps232239; LUBCHENCO J, 1981, ANNU REV ECOL SYST, V12, P405, DOI 10.1146/annurev.es.12.110181.002201; NELSON WG, 1979, J EXP MAR BIOL ECOL, V39, P231, DOI 10.1016/0022-0981(79)90129-1; NELSON WG, 1980, B MAR SCI, V30, P80; NITAO JK, 1991, ECOLOGY, V72, P1428, DOI 10.2307/1941115; Paradis E, 2004, BIOINFORMATICS, V20, P289, DOI 10.1093/bioinformatics/btg412; PAUL VJ, 2001, MARINE CHEM ECOLOGY; Poore AGB, 2001, EVOLUTION, V55, P68; Poore AGB, 2008, EVOLUTION, V62, P21, DOI 10.1111/j.1558-5646.2007.00261.x; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; RAUSHER MD, 1982, EVOLUTION, V36, P581, DOI 10.1111/j.1558-5646.1982.tb05078.x; RICHARDSON JP, 1979, J PHYCOL, V15, P22, DOI 10.1111/j.0022-3646.1979.00022.x; Sanford E, 2009, ECOLOGY, V90, P3108, DOI 10.1890/08-2055.1; SEARLES RB, 1984, HELGOLANDER MEERESUN, V38, P259, DOI 10.1007/BF01997484; Singer MC, 2010, EVOLUTION, V64, P921, DOI 10.1111/j.1558-5646.2009.00866.x; Sotka EE, 2003, EVOLUTION, V57, P2262; Sotka EE, 2005, ECOL LETT, V8, P448, DOI 10.1111/j.1461-0248.2004.00719.x; Sotka EE, 2002, ECOLOGY, V83, P2721, DOI 10.2307/3072010; Sotka EE, 2012, INTEGR COMP BIOL, V52, P538, DOI 10.1093/icb/ics084; Sotka EE, 2011, EVOL ECOL, V25, P1335, DOI 10.1007/s10682-011-9473-y; Sotka EE, 2009, INTEGR COMP BIOL, V49, P291, DOI 10.1093/icb/icp049; Sotka EE, 2009, BIOL BULL-US, V216, P75; Stachowicz JJ, 2007, ANNU REV ECOL EVOL S, V38, P739, DOI 10.1146/annurev.ecolsys.38.091206.095659; STEINBERG PD, 1984, SCIENCE, V223, P405, DOI 10.1126/science.223.4634.405; STENECK RS, 1982, MAR BIOL, V68, P299, DOI 10.1007/BF00409596; Stephens D, 2007, FORAGING BEHAV ECOLO, P576; STEPHENSON TA, 1952, J ECOL, V40, P1; STONER AW, 1980, MAR ECOL PROG SER, V3, P105, DOI 10.3354/meps003105; Strong DR., 1984, **DROPPED REF**; Targett NM, 2001, CRC MAR SCI, P391; Taylor RB, 2006, MAR BIOL, V149, P455, DOI 10.1007/s00227-006-0245-0; Thompson J.N., 2005, INTERSPEC INTERACT; THOMPSON JN, 1993, EVOLUTION, V47, P1585, DOI [10.2307/2410169, 10.1111/j.1558-5646.1993.tb02177.x]; Tilmon KJ, 2008, SPECIALIZATION, SPECIATION, AND RADIATION: THE EVOLUTIONARY BIOLOGY OF HERBIVOROUS INSECTS, pXIII; Traxler MA, 1999, OIKOS, V87, P239, DOI 10.2307/3546739; Vesakoski O, 2009, J EVOLUTION BIOL, V22, P1545, DOI 10.1111/j.1420-9101.2009.01767.x; VIRNSTEIN RW, 1984, ESTUARIES, V7, P310, DOI 10.2307/1351616; Wehling WF, 1997, OECOLOGIA, V111, P209, DOI 10.1007/s004420050227	68	11	11	0	81	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0029-8549	1432-1939		OECOLOGIA	Oecologia	AUG	2013	172	4					1071	1083		10.1007/s00442-012-2559-6			13	Ecology	Environmental Sciences & Ecology	188GF	WOS:000322180000015	23263529				2021-04-07	
J	Meslet-Cladiere, L; Delage, L; Leroux, CJJ; Goulitquer, S; Leblanc, C; Creis, E; Gall, EA; Stiger-Pouvreau, V; Czjzek, M; Potin, P				Meslet-Cladiere, Laurence; Delage, Ludovic; Leroux, Cedric J-J; Goulitquer, Sophie; Leblanc, Catherine; Creis, Emeline; Gall, Erwan Ar; Stiger-Pouvreau, Valerie; Czjzek, Mirjam; Potin, Philippe			Structure/Function Analysis of a Type III Polyketide Synthase in the Brown Alga Ectocarpus siliculosus Reveals a Biochemical Pathway in Phlorotannin Monomer Biosynthesis	PLANT CELL			English	Article							EXPRESSED SEQUENCE TAGS; SUBSTRATE-SPECIFICITY; PHENOLIC SUBSTANCES; EVOLUTION; LIPIDS; WALL; VARIABILITY; DERIVATIVES; METABOLISM; SECRETION	Brown algal phlorotannins are structural analogs of condensed tannins in terrestrial plants and, like plant phenols, they have numerous biological functions. Despite their importance in brown algae, phlorotannin biosynthetic pathways have been poorly characterized at the molecular level. We found that a predicted type III polyketide synthase in the genome of the brown alga Ectocarpus siliculosus, PKS1, catalyzes a major step in the biosynthetic pathway of phlorotannins (i.e., the synthesis of phloroglucinol monomers from malonyl-CoA). The crystal structure of PKS1 at 2.85-angstrom resolution provided a good quality electron density map showing a modified Cys residue, likely connected to a long chain acyl group. An additional pocket not found in other known type III PKSs contains a reaction product that might correspond to a phloroglucinol precursor. In vivo, we also found a positive correlation between the phloroglucinol content and the PKS III gene expression level in cells of a strain of Ectocarpus adapted to freshwater during its reacclimation to seawater. The evolution of the type III PKS gene family in Stramenopiles suggests a lateral gene transfer event from an actinobacterium.	[Meslet-Cladiere, Laurence; Delage, Ludovic; Goulitquer, Sophie; Leblanc, Catherine; Creis, Emeline; Czjzek, Mirjam; Potin, Philippe] CNRS, UMR 7139, Stn Biol Roscoff, F-29688 Roscoff, Brittany, France; [Meslet-Cladiere, Laurence; Delage, Ludovic; Goulitquer, Sophie; Leblanc, Catherine; Creis, Emeline; Czjzek, Mirjam; Potin, Philippe] Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, F-29688 Roscoff, Brittany, France; [Meslet-Cladiere, Laurence; Gall, Erwan Ar; Stiger-Pouvreau, Valerie] Univ Bretagne Occidentale, Univ Europeenne Bretagne, European Inst Marine Sci, Lab Sci Environm Marin,CNRS,UMR 6539, F-29280 Plouzane, France; [Leroux, Cedric J-J; Goulitquer, Sophie] Ctr Ressources Biol Marine, MetaboMer Core Facil, Stn Biol Roscoff, F-29688 Roscoff, Brittany, France; [Leroux, Cedric J-J; Goulitquer, Sophie] Ctr Ressources Biol Marine, Struct Biol Core Facil, Stn Biol Roscoff, F-29688 Roscoff, Brittany, France	Potin, P (corresponding author), CNRS, UMR 7139, Stn Biol Roscoff, F-29688 Roscoff, Brittany, France.	potin@sb-roscoff.fr		POTIN, Philippe/0000-0001-7358-6282; STIGER-POUVREAU, Valerie/0000-0003-3041-0468; LEROUX, Cedric/0000-0001-9225-1234	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Groupement d'Interet Scientifique Europole MER; project IDEALG [ANR-10-BTBR-04-02]; Universite Pierre et Marie Curie, Paris	Crystal structure determination was performed at the crystallography platform of the Station Biologique de Roscoff, supported by the Centre National de la Recherche Scientifique and Universite Pierre et Marie Curie, Paris 06. We are indebted to the staff of the ESRF (Grenoble, France), beamlines ID23-I and BM30A, for technical support during data collection and treatment. We thank Thierry Tonon for providing biological material and access to microarrays data before publication and for helpful discussions and advice, Fanny Gaillard for MALDI-TOF mass spectrometry analysis, and Laurence Dartevelle for technical assistance. This project was supported by a Grant-in-Aid for Scientific Research from Groupement d'Interet Scientifique Europole MER to V.S.-P. and P.P. (Phlorotann'ING project). L.M.-C. and S.G. were also partly supported by the project IDEALG (ANR-10-BTBR-04-02) "Investissements d'avenir, Biotechnologies-Bioresources." We also thank Groupement d'Interet Scientifique BiogenOuest for supporting MetaboMER facilities through the CORSAIRE metabolomics network.	Abe I, 2005, J AM CHEM SOC, V127, P12709, DOI 10.1021/ja053945v; Abe I, 2005, J AM CHEM SOC, V127, P1362, DOI 10.1021/ja0431206; Abe I, 2010, NAT PROD REP, V27, P809, DOI 10.1039/b909988n; Achkar J, 2005, J AM CHEM SOC, V127, P5332, DOI 10.1021/ja042340g; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Amsler CD, 2006, ADV BOT RES, V43, P1, DOI 10.1016/S0065-2296(05)43001-3; Arnold TM, 2002, J CHEM ECOL, V28, P1919, DOI 10.1023/A:1020737609151; Austin MB, 2004, J BIOL CHEM, V279, P45162, DOI 10.1074/jbc.M406567200; Austin MB, 2004, CHEM BIOL, V11, P1179, DOI 10.1016/j.chembiol.2004.05.024; Baharum H, 2011, MAR BIOTECHNOL, V13, P845, DOI 10.1007/s10126-010-9344-5; Bangera MG, 1999, J BACTERIOL, V181, P3155, DOI 10.1128/JB.181.10.3155-3163.1999; Berglin M, 2004, BIOMACROMOLECULES, V5, P2376, DOI 10.1021/bm0496864; Bitton R, 2007, MACROMOL BIOSCI, V7, P1280, DOI 10.1002/mabi.200700099; BOETTCHER AA, 1993, ECOLOGY, V74, P891, DOI 10.2307/1940814; Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; CHEN Y, 1997, OCEANOL LIMNOL SIN, V28, P225; Cock JM, 2011, J EXP BOT, V62, P2425, DOI 10.1093/jxb/err117; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; DAKORA FD, 1995, AUST J PLANT PHYSIOL, V22, P87, DOI 10.1071/PP9950087; Dittami SM, 2012, PLANT J, V71, P366, DOI 10.1111/j.1365-313X.2012.04982.x; Dittami SM, 2012, PROTIST, V163, P143, DOI 10.1016/j.protis.2011.07.004; El Hattab M, 2009, BIOCHEM SYST ECOL, V37, P55, DOI 10.1016/j.bse.2009.01.003; Emsley P, 2010, ACTA CRYSTALLOGR D, V66, P486, DOI 10.1107/S0907444910007493; Evans P, 2006, ACTA CRYSTALLOGR D, V62, P72, DOI 10.1107/S0907444905036693; Ferrer JL, 1999, NAT STRUCT BIOL, V6, P775, DOI 10.1038/11553; Funa N, 2002, BIOCHEM J, V367, P781, DOI 10.1042/BJ20020953; Funabashi M, 2008, J BIOL CHEM, V283, P13983, DOI 10.1074/jbc.M710461200; GERWICK W, 1982, PHYTOCHEMISTRY, V21, P633, DOI 10.1016/0031-9422(82)83154-3; Jez JM, 2002, P NATL ACAD SCI USA, V99, P5319, DOI 10.1073/pnas.082590499; Jez JM, 2001, BIOCHEMISTRY-US, V40, P14829, DOI 10.1021/bi015621z; Kim SS, 2010, PLANT CELL, V22, P4045, DOI 10.1105/tpc.110.080028; Koivikko R, 2005, J CHEM ECOL, V31, P195, DOI 10.1007/s10886-005-0984-2; Koivikko R, 2007, PHYTOCHEM ANALYSIS, V18, P326, DOI 10.1002/pca.986; Le Lann K, 2012, MAR ENVIRON RES, V80, P1, DOI 10.1016/j.marenvres.2012.05.011; Leslie AGW, 2006, ACTA CRYSTALLOGR D, V62, P48, DOI 10.1107/S0907444905039107; MATTHEWS BW, 1968, J MOL BIOL, V33, P491, DOI 10.1016/0022-2836(68)90205-2; McClintock JB, 2001, MARINE CHEM ECOLOGY; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Mizuuchi Y, 2008, BIOL PHARM BULL, V31, P2205, DOI 10.1248/bpb.31.2205; Murshudov GN, 1997, ACTA CRYSTALLOGR D, V53, P240, DOI 10.1107/S0907444996012255; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Parys S, 2007, J NAT PROD, V70, P1865, DOI 10.1021/np070302f; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Pelletreau K. N., 2008, P121, DOI 10.1007/978-3-540-74181-7_6; Pelletreau K.N., 2008, THESIS U DELAWARE NE; Perrakis A, 1999, NAT STRUCT BIOL, V6, P458, DOI 10.1038/8263; Pfeifer BA, 2001, MICROBIOL MOL BIOL R, V65, P106, DOI 10.1128/MMBR.65.1.106-118.2001; Potterton E, 2003, ACTA CRYSTALLOGR D, V59, P1131, DOI 10.1107/S0907444903008126; Potterton L, 2004, ACTA CRYSTALLOGR D, V60, P2288, DOI 10.1107/S0907444904023716; Ragan M. A., 1986, PROG PHYCOL RES, V4, P129; Sankaranarayanan R, 2004, NAT STRUCT MOL BIOL, V11, P894, DOI 10.1038/nsmb809; Sankaranarayanan R, 2006, NAT CHEM BIOL, V2, P451, DOI 10.1038/nchembio0906-451; Schoenwaelder MEA, 1998, J PHYCOL, V34, P969, DOI 10.1046/j.1529-8817.1998.340969.x; Shibata T, 2006, J APPL PHYCOL, V18, P787, DOI 10.1007/s10811-006-9094-y; SIEBURTH JM, 1965, NATURE, V208, P52, DOI 10.1038/208052a0; Stengel DB, 2011, BIOTECHNOL ADV, V29, P483, DOI 10.1016/j.biotechadv.2011.05.016; Sterck L, 2012, NAT METHODS, V9, P1041, DOI 10.1038/nmeth.2242; Studier FW, 2005, PROTEIN EXPRES PURIF, V41, P207, DOI 10.1016/j.pep.2005.01.016; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Thomas NV, 2011, ENVIRON TOXICOL PHAR, V32, P325, DOI 10.1016/j.etap.2011.09.004; Toth GB, 2000, P NATL ACAD SCI USA, V97, P14418, DOI 10.1073/pnas.250226997; Vagin A, 2010, ACTA CRYSTALLOGR D, V66, P22, DOI 10.1107/S0907444909042589; Waddell SJ, 2005, LETT APPL MICROBIOL, V40, P201, DOI 10.1111/j.1472-765X.2005.01659.x; Waterman P.G., 1994, METHODS ECOLOGY SERI; Wisespongpand P, 2003, J APPL PHYCOL, V15, P225, DOI 10.1023/A:1023831131735; Wong TKM, 2007, J PHYCOL, V43, P528, DOI 10.1111/j.1529-8817.2007.00349.x; Zha WJ, 2006, J BIOL CHEM, V281, P32036, DOI 10.1074/jbc.M606500200	69	43	45	0	51	AMER SOC PLANT BIOLOGISTS	ROCKVILLE	15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA	1040-4651	1532-298X		PLANT CELL	Plant Cell	AUG	2013	25	8					3089	3103		10.1105/tpc.113.111336			15	Biochemistry & Molecular Biology; Plant Sciences; Cell Biology	Biochemistry & Molecular Biology; Plant Sciences; Cell Biology	224VX	WOS:000324920500028	23983220	Green Published, Bronze			2021-04-07	
J	Raven, JA				Raven, John A.			Iron acquisition and allocation in stramenopile algae	JOURNAL OF EXPERIMENTAL BOTANY			English	Article						allocation; Bacillariophyceae; deficiency; Ectocarpus; iron; Pelagophyceae; storage; transport	VERTICAL MIGRATION BEHAVIOR; ELEMENTAL STOICHIOMETRY; DOMOIC ACID; EVOLUTIONARY INHERITANCE; PHYSIOLOGICAL-RESPONSES; USE EFFICIENCIES; MARINE; GROWTH; LIGHT; PHOSPHATE	The essential element iron has a low biological availability in the surface ocean where photosynthetic organisms live. Recent advances in our understanding of iron acquisition mechanisms in brown algae and diatoms (stramenopile algae) show the importance of the reduction of ferric to ferrous iron prior to, or during, transport in the uptake process. The uses of iron in photosynthetic stramenopiles resembles that in other oxygenic organisms, although (with the exception of the diatom Thalassiosira oceanica from an iron-deficient part of the ocean) they lack plastocyanin, instead using cytochrome c(6), This same diatom further economizes genotypically on the use of iron in photosynthesis by decreasing the expression of photosystem I, cytochrome c(6), and the cytochrome b(6)f complex per cell and per photosystem II relative to the coastal Thalassiosira pseudonana; similar changes occur phenotypically in response to iron deficiency in other diatoms such as Phaeodactylum tricornutum. In some diatoms grown under iron-limiting conditions, essentially all of the iron in the cells can be accounted for by the iron occurring in catalytic proteins. However, stramenopiles can store iron. Genomic studies show that pennate, but not centric, diatoms have the iron storage protein ferritin. While Mssbauer and X-ray analysis of Fe-57-labelled Ectocarpus siliculosus shows iron in an amorphous mineral phase resembling the core of ferritin, the genome shows no protein with significant sequence similarity to ferritin.	[Raven, John A.] Univ Dundee, James Hutton Inst, Div Plant Sci, Dundee DD2 5DA, Scotland; [Raven, John A.] Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia	Raven, JA (corresponding author), Univ Dundee, James Hutton Inst, Div Plant Sci, Dundee DD2 5DA, Scotland.	j.a.raven@dundee.ac.uk					Allen AE, 2008, P NATL ACAD SCI USA, V105, P10438, DOI 10.1073/pnas.0711370105; ANDERSON MA, 1982, LIMNOL OCEANOGR, V27, P789, DOI 10.4319/lo.1982.27.5.0789; Ashkenazy Y, 2013, NATURE, V495, P90, DOI 10.1038/nature11894; Bekker A, 2004, NATURE, V427, P117, DOI 10.1038/nature02260; Blaby-Haas CE, 2012, BBA-MOL CELL RES, V1823, P1531, DOI 10.1016/j.bbamcr.2012.04.010; Blain S, 2008, BIOGEOSCIENCES, V5, P269, DOI 10.5194/bg-5-269-2008; Blank CE, 2010, GEOBIOLOGY, V8, P1, DOI 10.1111/j.1472-4669.2009.00220.x; Bottger LH, 2013, J EXP BIOL, V63, p[2013, 5763]; Boyd CM, 2002, MAR BIOL, V141, P605, DOI 10.1007/s00227-002-0872-z; Boyd PW, 2008, MAR ECOL PROG SER, V364, P213, DOI 10.3354/meps07541; Brown JW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012759; Cooke RRM, 2004, NEW ZEAL J MAR FRESH, V38, P73, DOI 10.1080/00288330.2004.9517219; Crowe SA, 2008, P NATL ACAD SCI USA, V105, P15938, DOI 10.1073/pnas.0805313105; Desai DK, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00362; Falkowski PG, 2004, SCIENCE, V305, P354, DOI 10.1126/science.1095964; Finkel ZV, 2006, LIMNOL OCEANOGR, V51, P2690, DOI 10.4319/lo.2006.51.6.2690; Graham J.E., 2009, ALGAE; Handy SM, 2005, AQUAT MICROB ECOL, V40, P121, DOI 10.3354/ame040121; Ho TY, 2003, J PHYCOL, V39, P1145, DOI 10.1111/j.0022-3646.2003.03-090.x; HUDSON RJM, 1993, DEEP-SEA RES PT I, V40, P129, DOI 10.1016/0967-0637(93)90057-A; Kimura T, 1999, J APPL PHYCOL, V11, P301, DOI 10.1023/A:1008196308564; Kustka AB, 2007, J PHYCOL, V43, P715, DOI 10.1111/j.1529-8817.2007.00359.x; Lommer M, 2012, GENOME BIOL, V13, DOI 10.1186/gb-2012-13-7-r66; MACFARLANE JJ, 1990, PLANT CELL ENVIRON, V13, P1, DOI 10.1111/j.1365-3040.1990.tb01294.x; Maldonado MT, 1996, MAR ECOL PROG SER, V141, P161, DOI 10.3354/meps141161; Maldonado MT, 2002, LIMNOL OCEANOGR, V47, P515, DOI 10.4319/lo.2002.47.2.0515; Marchetti A, 2012, P NATL ACAD SCI USA, V109, pE317, DOI 10.1073/pnas.1118408109; Marchetti A, 2009, NATURE, V457, P467, DOI 10.1038/nature07539; MARTIN JH, 1991, LIMNOL OCEANOGR, V36, P1793, DOI 10.4319/lo.1991.36.8.1793; McKay RML, 2000, J PHYCOL, V36, P669, DOI 10.1046/j.1529-8817.2000.99125.x; MCLACHLAN J, 1977, PHYCOLOGIA, V16, P329, DOI 10.2216/i0031-8884-16-3-329.1; Mikucki JA, 2009, SCIENCE, V324, P397, DOI 10.1126/science.1167350; Morrissey J, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00043; Nichols DB, 2001, HARMFUL ALGAL BLOOMS, P340; Nuester J, 2012, J PHYCOL, V48, P626, DOI 10.1111/j.1529-8817.2012.01165.x; Peers G, 2006, NATURE, V441, P341, DOI 10.1038/nature04630; Planavsky NJ, 2010, NATURE, V467, P1088, DOI 10.1038/nature09485; Powers L, 2012, HARMFUL ALGAE, V13, P95, DOI 10.1016/j.hal.2011.10.007; Quigg A, 2003, NATURE, V425, P291, DOI 10.1038/nature01953; Quigg A, 2011, P ROY SOC B-BIOL SCI, V278, P526, DOI 10.1098/rspb.2010.1356; Raven JA, 1999, PHOTOSYNTH RES, V60, P111, DOI 10.1023/A:1006282714942; RAVEN JA, 1990, NEW PHYTOL, V116, P1, DOI 10.1111/j.1469-8137.1990.tb00536.x; RAVEN JA, 1988, NEW PHYTOL, V109, P279, DOI 10.1111/j.1469-8137.1988.tb04196.x; Raven JA, 2012, CURR BIOL, V22, pR682, DOI 10.1016/j.cub.2012.07.030; Raven JA, 2009, FUNCT PLANT BIOL, V36, P505, DOI 10.1071/FP09087; Ribalet F, 2010, P NATL ACAD SCI USA, V107, P16571, DOI 10.1073/pnas.1005638107; Rue E, 2001, MAR CHEM, V76, P127, DOI 10.1016/S0304-4203(01)00053-6; Saito MA, 2003, INORG CHIM ACTA, V356, P308, DOI 10.1016/S0020-1693(03)00442-0; Sedwick PN, 2005, GLOBAL BIOGEOCHEM CY, V19, DOI 10.1029/2004GB002445; Smetacek V, 2012, NATURE, V487, P313, DOI 10.1038/nature11229; Strzepek RF, 2004, NATURE, V431, P689, DOI 10.1038/nature02954; Strzepek RF, 2000, MARINE ECOLOGY PROGR, V206, P106; Strzepek RF, 2012, LIMNOL OCEANOGR, V57, P1182, DOI 10.4319/lo.2012.57.4.1182; Strzepek RF, 2011, LIMNOL OCEANOGR, V56, P1983, DOI 10.4319/lo.2011.56.6.1983; SUNDA WG, 1995, MAR CHEM, V50, P189, DOI 10.1016/0304-4203(95)00035-P; SUNDA WG, 1991, NATURE, V351, P55, DOI 10.1038/351055a0; Sunda WG, 1997, NATURE, V390, P389, DOI 10.1038/37093; Sunda WG, 2011, LIMNOL OCEANOGR, V56, P1475, DOI 10.4319/lo.2011.56.4.1475; Sutak R, 2012, PLANT PHYSIOL, V160, P2271, DOI 10.1104/pp.112.204156; SUZUKI Y, 1995, PHYCOLOGIA, V34, P201, DOI 10.2216/i0031-8884-34-3-201.1; Timmermans KR, 2005, J SEA RES, V53, P109, DOI 10.1016/j.seares.2004.05.003; Trainer VL, 2012, HARMFUL ALGAE, V14, P271, DOI 10.1016/j.hal.2011.10.025; Trick CG, 2010, P NATL ACAD SCI USA, V107, P5887, DOI 10.1073/pnas.0910579107; VANDENHOEK C, 1995, INTRO PHYCOLOGY; Villareal TA, 1999, J PHYCOL, V35, P896, DOI 10.1046/j.1529-8817.1999.3550896.x; VILLAREAL TA, 1993, NATURE, V363, P709, DOI 10.1038/363709a0; Villareal TA, 1996, J PLANKTON RES, V18, P1103, DOI 10.1093/plankt/18.7.1103; WADA M, 1985, PLANT CELL PHYSIOL, V26, P431, DOI 10.1093/oxfordjournals.pcp.a076926; WADE VJ, 1993, BIOCHIM BIOPHYS ACTA, V1161, P91, DOI 10.1016/0167-4838(93)90201-2; WATANABE M, 1988, J PHYCOL, V24, P22; WATANABE M M, 1983, Japanese Journal of Phycology, V31, P161; Wells ML, 2005, LIMNOL OCEANOGR, V50, P1908, DOI 10.4319/lo.2005.50.6.1908; Whitney LP, 2011, FRONT MICROBIOL, V2, DOI 10.3389/fmicb.2011.00234; Williams RJP, 2012, EVOLUTION'S DESTINY: CO-EVOLVING CHEMISTRY OF THE ENVIRONMENT AND LIFE, P1, DOI 10.1039/9781849735599; WILLIAMS RJP, 1982, FEBS LETT, V140, P3, DOI 10.1016/0014-5793(82)80508-5; WILLIAMS RJP, 1981, PROC R SOC SER B-BIO, V213, P361, DOI 10.1098/rspb.1981.0071; Yang EC, 2012, PROTIST, V163, P217, DOI 10.1016/j.protis.2011.08.001; Zerkle AL, 2006, GEOBIOLOGY, V4, P286	78	21	21	1	54	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0022-0957	1460-2431		J EXP BOT	J. Exp. Bot.	MAY	2013	64	8					2119	2127		10.1093/jxb/ert121			9	Plant Sciences	Plant Sciences	151AO	WOS:000319433200001	23658428	Bronze			2021-04-07	
J	Konotchick, T; Dupont, CL; Valas, RE; Badger, JH; Allen, AE				Konotchick, Talina; Dupont, Christopher L.; Valas, Ruben E.; Badger, Jonathan H.; Allen, Andrew E.			Transcriptomic analysis of metabolic function in the giant kelp, Macrocystis pyrifera, across depth and season	NEW PHYTOLOGIST			English	Article						comparative genomics; light harvesting complex; Macrocystis pyrifera (giant kelp); Phaeophyceae; quantitative PCR; RNA-Seq; transcriptomics; water-column gradients	LAMINARIA-DIGITATA PHAEOPHYCEAE; ALGA ECTOCARPUS-SILICULOSUS; SEQUENCE TAG ANALYSIS; IN-SITU; SOUTHERN-CALIFORNIA; EUKARYOTIC GENOMES; CARBON METABOLISM; BINDING PROTEINS; BROWN-ALGAE; GENES	To increase knowledge of transcript diversity for the giant kelp, Macrocystis pyrifera, and assess gene expression across naturally occurring depth gradients in light, temperature and nutrients, we sequenced four cDNA libraries created from blades collected at the sea surface and at 18m depth during the winter and summer. Comparative genomics cluster analyses revealed novel gene families (clusters) in existing brown alga expressed sequence tag data compared with other related algal groups, a pattern also seen with the addition of M.pyrifera sequences. Assembly of 228Mbp of sequence generated c. 9000 isotigs and c. 12000 open reading frames. Annotations were assigned using families of hidden Markov models for c. 11% of open reading frames; M.pyrifera had highest similarity to other members of the Phaeophyceae, namely Ectocarpus siliculosus and Laminaria digitata. Quantitative polymerase chain reaction of transcript targets verified depth-related differences in gene expression; stress response and light-harvesting transcripts, especially members of the LI818 (also known as LHCSR) family, showed high expression in the surface compared with 18m depth, while some nitrogen acquisition transcripts (e.g. nitrite reductase) were upregulated at depth compared with the surface, supporting a conceptual biological model of depth-dependent physiology.	[Konotchick, Talina; Dupont, Christopher L.; Valas, Ruben E.; Badger, Jonathan H.; Allen, Andrew E.] J Craig Venter Inst, San Diego, CA USA	Allen, AE (corresponding author), J Craig Venter Inst, 10355 Sci Ctr Dr, San Diego, CA USA.	aallen@jcvi.org	Allen, Andrew E/C-4896-2012	Allen, Andrew E/0000-0001-5911-6081	National Science FoundationNational Science Foundation (NSF); Mia J. Tegner Fellowship; Sigma Xi Grant-in-Aid of Research; Beyster Foundation;  [NSF-OCE-1136477];  [NSF-MCB-1024913];  [DOE-DE-SC0006719]	The work was made possible by funding support from a National Science Foundation Graduate Research Fellowship, the Mia J. Tegner Fellowship for Coastal Ecology Fieldwork, and a Sigma Xi Grant-in-Aid of Research (T. K.). Work in the laboratory of A. E. A. was supported by NSF-OCE-1136477, NSF-MCB-1024913, DOE-DE-SC0006719, and by the Beyster Foundation. The Bioinformatics Ghent Online Genome Annotation Service (BOGAS) for the E. siliculosus genome annotation greatly aided analyses of M. pyrifera. We thank Scripps Institution of Oceanography's scientific diving program for diving support in the field. We acknowledge the efforts of J. Bai, H. Zheng and M. Lehmann. Comments from and discussion with G. Peers, J. Leichter, L. Levin and three anonymous reviewers significantly improved the manuscript.	APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chen F, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000383; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Colombo-Pallotta MF, 2006, J PHYCOL, V42, P1225, DOI 10.1111/j.1529-8817.2006.00287.x; Crawford N.M., 2000, BIOCH MOL BIOL PLANT, P786; Crepineau F, 2000, PLANT MOL BIOL, V43, P503, DOI 10.1023/A:1006489920808; DAYTON PK, 1985, ANNU REV ECOL SYST, V16, P215, DOI 10.1146/annurev.es.16.110185.001243; Dayton PK, 1999, ECOL MONOGR, V69, P219, DOI 10.1890/0012-9615(1999)069[0219:TASSOK]2.0.CO;2; DEAN TA, 1985, ESTUAR COAST SHELF S, V21, P835, DOI 10.1016/0272-7714(85)90077-0; Dittami SM, 2010, BMC EVOL BIOL, V10, DOI 10.1186/1471-2148-10-365; DOHLER G, 1995, J PHOTOCH PHOTOBIO B, V30, P179, DOI 10.1016/1011-1344(95)07189-9; Douzery EJP, 2004, P NATL ACAD SCI USA, V101, P15386, DOI 10.1073/pnas.0403984101; Garcia Mendoza E, 2007, NEW PHYTOL, V173, P526; Garcia Mendoza E, 2011, J PHOTOCH PHOTOBIO B, V104, P377; GERARD VA, 1982, MAR BIOL, V66, P27, DOI 10.1007/BF00397251; GERARD VA, 1986, MAR BIOL, V90, P473, DOI 10.1007/BF00428571; GERARD VA, 1984, MAR BIOL, V84, P189, DOI 10.1007/BF00393004; Gobler CJ, 2011, P NATL ACAD SCI USA, V108, P4352, DOI 10.1073/pnas.1016106108; Gomez-Alvarez V, 2009, ISME J, V3, P1314, DOI 10.1038/ismej.2009.72; GREEN BR, 1991, TRENDS BIOCHEM SCI, V16, P181, DOI 10.1016/0968-0004(91)90072-4; Green BR, 1996, ANNU REV PLANT PHYS, V47, P685, DOI 10.1146/annurev.arplant.47.1.685; Ishikawa T, 2008, BIOSCI BIOTECH BIOCH, V72, P1143, DOI 10.1271/bbb.80062; JACKSON GA, 1977, LIMNOL OCEANOGR, V22, P979, DOI 10.4319/lo.1977.22.6.0979; Jackson GA, 1997, CONT SHELF RES, V17, P1913, DOI 10.1016/S0278-4343(97)00054-X; Jamers A, 2009, AQUAT TOXICOL, V92, P114, DOI 10.1016/j.aquatox.2009.02.012; KAMYKOWSKI D, 1986, DEEP-SEA RES, V33, P89, DOI 10.1016/0198-0149(86)90109-3; Kasukabe Y, 2004, PLANT CELL PHYSIOL, V45, P712, DOI 10.1093/pcp/pch083; Keeling PJ, 2005, TRENDS ECOL EVOL, V20, P670, DOI 10.1016/j.tree.2005.09.005; Konotchick T, 2012, ESTUAR COAST SHELF S, V106, P85, DOI 10.1016/j.ecss.2012.04.026; La Barre S, 2010, MAR DRUGS, V8, P988, DOI 10.3390/md8040988; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; LEGALL Y, 1993, PROTOPLASMA, V173, P123; Li L, 2003, GENOME RES, V13, P2178, DOI 10.1101/gr.1224503; MANLEY SL, 1983, J PHYCOL, V19, P118, DOI 10.1111/j.0022-3646.1983.00118.x; MANN KH, 1973, SCIENCE, V182, P975, DOI 10.1126/science.182.4116.975; McFARLAND WILLIAM N., 1959, PUBL INST MARINE SCI, V6, P109; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Moulin P, 1999, J PHYCOL, V35, P1237, DOI 10.1046/j.1529-8817.1999.3561237.x; Nam S, 2011, GEOPHYS RES LETT, V38, DOI 10.1029/2011GL049549; PARKER BC, 1966, J PHYCOL, V2, P38, DOI 10.1111/j.1529-8817.1966.tb04590.x; Peers G, 2009, NATURE, V462, P518, DOI 10.1038/nature08587; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Phillips N, 2008, J PHYCOL, V44, P15, DOI 10.1111/j.1529-8817.2007.00435.x; Rho MN, 2010, NUCLEIC ACIDS RES, V38, DOI 10.1093/nar/gkq747; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Rozen S, 2000, Methods Mol Biol, V132, P365; SARGENT MC, 1952, AM J BOT, V39, P99, DOI 10.2307/2438175; Savard F, 1996, PLANT MOL BIOL, V32, P461, DOI 10.1007/BF00019098; SMITH BM, 1987, PLANT PHYSIOL, V84, P1325, DOI 10.1104/pp.84.4.1325; Teal TK, 2010, COLD SPRING HARBOR P, V2010; TOWLE DW, 1973, LIMNOL OCEANOGR, V18, P155, DOI 10.4319/lo.1973.18.1.0155; Vandesompele J, 2002, GENOME BIOL, V3, DOI 10.1186/gb-2002-3-7-research0034; Waaland JR, 2004, J PHYCOL, V40, P26, DOI 10.1111/j.0022-3646.2003.03-148.x; WING SR, 1993, LIMNOL OCEANOGR, V38, P396, DOI 10.4319/lo.1993.38.2.0396; Wu ZJ, 2010, BMC BIOINFORMATICS, V11, DOI 10.1186/1471-2105-11-564; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; ZIMMERMAN RC, 1986, MAR ECOL PROG SER, V27, P277, DOI 10.3354/meps027277	61	29	30	0	59	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X			NEW PHYTOL	New Phytol.	APR	2013	198	2					398	407		10.1111/nph.12160			10	Plant Sciences	Plant Sciences	112XV	WOS:000316629500009	23488966	Green Published, Bronze			2021-04-07	
J	Uji, T; Mizuta, H; Saga, N				Uji, Toshiki; Mizuta, Hiroyuki; Saga, Naotsune			Characterization of the Sporophyte-Preferential Gene Promoter from the Red Alga Porphyra yezoensis Using Transient Gene Expression	MARINE BIOTECHNOLOGY			English	Article						Gametophyte; Marine macroalgae; Porphyra yezoensis; Promoter analysis; Sporophyte	CIS-ACTING ELEMENT; REGULATORY ELEMENTS; SEQUENCE; TRANSCRIPTION; GAMETOPHYTE; EUKARYOTES; GENERATION; RHODOPHYTA; ECTOCARPUS; EVOLUTION	The life cycle of plants entails an alternation of generations, the diploid sporophyte and haploid gametophyte stages. There is little information about the characteristics of gene expression during each phase of marine macroalgae. Promoter analysis is a useful method for understanding transcriptional regulation; however, there is no report of promoter analyses in marine macroalgae. In this study, with the aim of elucidating the differences in the transcriptional regulatory mechanisms between the gametophyte and sporophyte stages in the marine red alga Porphyra yezoensis, we isolated the promoter from the sporophyte preferentially expressed gene PyKPA1, which encodes a sodium pump, and analyzed its promoter using a transient gene expression system with a synthetic beta-glucuronidase (PyGUS) reporter. The deletion of -1432 to -768 relative to the transcription start site resulted in decreased GUS activity in sporophytes. In contrast, deletion from -767 to -527 increased GUS activity in gametophytes. Gain-of-function analyses showed that the -1432 to -760 region enhanced the GUS activity of a heterologous promoter in sporophytes, whereas the -767 to -510 region repressed it in gametophytes. Further mutation and gain-of-function analyses of the -767 to -510 region revealed that a 20-bp GC-rich sequence (-633 to -614) is responsible for the gametophyte-specific repressed expression. These results showed that the sporophyte-specific positive regulatory region and gametophyte-specific negative regulatory sequence play a crucial role in the preferential expression of PyKPA1 in P. yezoensis sporophytes.	[Uji, Toshiki; Mizuta, Hiroyuki; Saga, Naotsune] Hokkaido Univ, Fac Fisheries Sci, Hakodate, Hokkaido 0418611, Japan	Saga, N (corresponding author), Hokkaido Univ, Fac Fisheries Sci, Hakodate, Hokkaido 0418611, Japan.	nsaga@fish.hokudai.ac.jp			Ministry of Education, Culture, Sports, Science, and Technology, JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)	This study was supported in part by the Regional Innovation Cluster Program (global type) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan to N. S.	Asamizu E, 2003, J PHYCOL, V39, P923, DOI 10.1046/j.1529-8817.2003.03003.x; Butterfield NJ, 2000, PALEOBIOLOGY, V26, P386, DOI 10.1666/0094-8373(2000)026<0386:BPNGNS>2.0.CO;2; CAMPBELL SE, 1980, PHYCOLOGIA, V19, P25, DOI 10.2216/i0031-8884-19-1-25.1; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Cole KM, 1975, PHYCOLOGIA, V16, P205; EYAL Y, 1995, PLANT CELL, V7, P373, DOI 10.1105/tpc.7.3.373; Fukuda S, 2008, PLANT SCI, V174, P329, DOI 10.1016/j.plantsci.2007.12.006; Hamilton DA, 1998, PLANT MOL BIOL, V38, P663, DOI 10.1023/A:1006083725102; Hirata R, 2011, MAR BIOTECHNOL, V13, P1038, DOI 10.1007/s10126-011-9367-6; JEFFERSON RA, 1987, EMBO J, V6, P3901; Jiang C, 1996, PLANT MOL BIOL, V30, P679, DOI 10.1007/BF00049344; MAIER UG, 1987, EMBO J, V6, P17, DOI 10.1002/j.1460-2075.1987.tb04712.x; Mikami K, 2011, TRANSIENT TRANSFORMA, P241; Mikula M, 2010, DNA RES, V17, P245, DOI 10.1093/dnares/dsq016; MUKAI LS, 1981, J PHYCOL, V17, P192, DOI 10.1111/j.0022-3646.1981.00192.x; Nikaido I, 2000, DNA RES, V7, P223, DOI 10.1093/dnares/7.3.223; Ohme-Takagi M, 2000, PLANT CELL PHYSIOL, V41, P1187, DOI 10.1093/pcp/pcd057; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Reyes JC, 2006, CURR OPIN PLANT BIOL, V9, P21, DOI 10.1016/j.pbi.2005.11.010; Rogers HJ, 2001, PLANT MOL BIOL, V45, P577, DOI 10.1023/A:1010695226241; Saga N, 2002, FISH SCI S, V68, P1075, DOI DOI 10.2331/fishsci.68.sup2_1075; Sharoni AM, 2011, PLANT CELL PHYSIOL, V52, P344, DOI 10.1093/pcp/pcq196; TWELL D, 1991, GENE DEV, V5, P496, DOI 10.1101/gad.5.3.496; Uji T, 2012, MOL BIOL REP, V39, P7973, DOI 10.1007/s11033-012-1643-7; Uji T, 2010, MAR BIOTECHNOL, V12, P150, DOI 10.1007/s10126-009-9210-5; Vickers CE, 2006, PLANT MOL BIOL, V62, P195, DOI 10.1007/s11103-006-9014-1; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zhou P, 2010, PLANT CELL REP, V29, P503, DOI 10.1007/s00299-010-0839-3	28	3	4	0	34	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	1436-2228			MAR BIOTECHNOL	Mar. Biotechnol.	APR	2013	15	2					188	196		10.1007/s10126-012-9475-y			9	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	090OB	WOS:000314987500006	22865243				2021-04-07	
J	Fu, G; Nagasato, C; Ito, T; Muller, DG; Motomura, T				Fu, Gang; Nagasato, Chikako; Ito, Toshiaki; Muller, Dieter G.; Motomura, Taizo			Ultrastructural analysis of flagellar development in plurilocular sporangia of Ectocarpus siliculosus (Phaeophyceae)	PROTOPLASMA			English	Article						Brown algae; Ectocarpus; Flagellar differentiation; Transmission electron microscopy; Electron tomography	ALGA PYLAIELLA-LITTORALIS; SCYTOSIPHON-LOMENTARIA SCYTOSIPHONALES; ELECTRON-MICROSCOPE OBSERVATIONS; FINE-STRUCTURE; MALE GAMETE; REPRODUCTION; AUTOFLUORESCENCE; CENTROSOME; ZOOSPOROGENESIS; GAMETOGENESIS	Flagellar development in the plurilocular zoidangia of sporophytes of the brown alga Ectocarpus siliculosus was analyzed in detail using transmission electron microscopy and electron tomography. A series of cell divisions in the plurilocular zoidangia produced the spore-mother cells. In these cells, the centrioles differentiated into flagellar basal bodies with basal plates at their distal ends and attached to the plasma membrane. The plasma membrane formed a depression (flagellar pocket) into where the flagella elongated and in which variously sized vesicles and cytoplasmic fragments accumulated. The anterior and posterior flagella started elongating simultaneously, and the vesicles and cytoplasmic fragments in the flagellar pocket fused to the flagellar membranes. The two flagella (anterior and posterior) could be clearly distinguished from each other at the initial stage of their development by differences in length, diameter and the appendage flagellar rootlets. Flagella continued to elongate in the flagellar pocket and maintained their mutually parallel arrangement as the flagellar pocket gradually changed position. In mature zoids, the basal part of the posterior flagellum (paraflagellar body) characteristically became swollen and faced the eyespot region. Electron dense materials accumulated between the axoneme and the flagellar membrane, and crystallized materials could also be observed in the swollen region. Before liberation of the zoospores from the plurilocular zoidangia, mastigoneme attachment was restricted to the distal region of the anterior flagellum. Structures just below the flagellar membrane that connected to the mastigonemes were clearly visible by electron tomography.	[Fu, Gang] Hokkaido Univ, Grad Sch Environm Sci, Sapporo, Hokkaido 0600810, Japan; [Fu, Gang; Nagasato, Chikako; Motomura, Taizo] Hokkaido Univ, Muroran Marine Stn, Field Sci Ctr No Biosphere, Muroran, Hokkaido 0510003, Japan; [Ito, Toshiaki] Hokkaido Univ, Res Fac Agr, Electron Microscope Lab, Sapporo, Hokkaido 0608589, Japan; [Muller, Dieter G.] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany	Motomura, T (corresponding author), Hokkaido Univ, Muroran Marine Stn, Field Sci Ctr No Biosphere, Muroran, Hokkaido 0510003, Japan.	motomura@fsc.hokudai.ac.jp			Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [22570084, 24112701] Funding Source: KAKEN		Andersen RA, 2004, AM J BOT, V91, P1508, DOI 10.3732/ajb.91.10.1508; BAKER JRJ, 1973, PROTOPLASMA, V77, P181, DOI 10.1007/BF01276756; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BEECH PL, 1991, PROTOPLASMA, V164, P23, DOI 10.1007/BF01320812; BOUCK GB, 1969, J CELL BIOL, V40, P446, DOI 10.1083/jcb.40.2.446; Bui KH, 2011, J SYNCHROTRON RADIAT, V18, P2, DOI 10.1107/S0909049510036812; Chang P, 2000, NAT CELL BIOL, V2, P30; CLAYTON MN, 1989, SYST ASSOC SPEC VOL, V38, P229; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cole DG, 2009, CELL, V137, P784, DOI 10.1016/j.cell.2009.04.053; COLEMAN AW, 1988, J PHYCOL, V24, P118; Fujita S, 2005, EUR J PHYCOL, V40, P159, DOI 10.1080/09670260500063193; GELLER A, 1981, J EXP BIOL, V92, P53; Green JC, 1989, CHROMOPHYTE ALGAE PR, P429; HENRY EC, 1982, J PHYCOL, V18, P550; Hoyer-Fender S, 2010, SEMIN CELL DEV BIOL, V21, P142, DOI 10.1016/j.semcdb.2009.07.002; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; KAWAI H, 1988, J PHYCOL, V24, P114; KAWAI H, 1992, BOT MAG TOKYO, V105, P171, DOI 10.1007/BF02489413; Kremer JR, 1996, J STRUCT BIOL, V116, P71, DOI 10.1006/jsbi.1996.0013; LACLAIRE JW, 1978, PROTOPLASMA, V97, P93, DOI 10.1007/BF01276686; Lacomble S, 2009, J CELL SCI, V122, P1081, DOI 10.1242/jcs.045740; LANGE BMH, 1995, J CELL BIOL, V130, P919, DOI 10.1083/jcb.130.4.919; LOFTHOUSE PF, 1975, PROTOPLASMA, V84, P83, DOI 10.1007/BF02075945; LOISEAUX S, 1973, J PHYCOL, V9, P277; LOISEAUX S, 1970, T AM MICROSC SOC, V89, P524, DOI 10.2307/3224562; Maier I, 1997, EUR J PHYCOL, V32, P255; Maier I, 1997, EUR J PHYCOL, V32, P241; MANTON I, 1953, J EXP BOT, V4, P319, DOI 10.1093/jxb/4.3.319; MANTON I, 1950, NATURE, V166, P973, DOI 10.1038/166973a0; MANTON I, 1956, J EXP BOT, V7, P416, DOI 10.1093/jxb/7.3.416; MANTON I, 1951, J EXP BOT, V2, P242, DOI 10.1093/jxb/2.2.242; MANTON I., 1964, NEW PHYTQL, V63, P244, DOI 10.1111/j.1469-8137.1964.tb07377.x; MARKEY DR, 1976, PROTOPLASMA, V88, P175, DOI 10.1007/BF01283244; MARKEY DR, 1977, J ULTRA MOL STRUCT R, V59, P173, DOI 10.1016/S0022-5320(77)80077-4; MARKEY DR, 1976, PROTOPLASMA, V88, P147, DOI 10.1007/BF01283243; MARKEY DR, 1975, PROTOPLASMA, V85, P219, DOI 10.1007/BF01567948; Marshall WF, 2002, TRENDS CELL BIOL, V12, P414, DOI 10.1016/S0962-8924(02)02341-3; Mastronarde DN, 1997, J STRUCT BIOL, V120, P343, DOI 10.1006/jsbi.1997.3919; Matsunaga S, 2010, PHOTOCHEM PHOTOBIOL, V86, P374, DOI 10.1111/j.1751-1097.2009.00676.x; McIntosh R, 2005, TRENDS CELL BIOL, V15, P43, DOI 10.1016/j.tcb.2004.11.009; Melkonian M., 1987, P102; MOTOMURA T, 1993, SCI PAP I ALG RES HO, V9, P1; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; Nagasato C, 2002, J CELL SCI, V115, P2541; Nicastro D, 2005, P NATL ACAD SCI USA, V102, P15889, DOI 10.1073/pnas.0508274102; Nicastro D, 2006, SCIENCE, V313, P944, DOI 10.1126/science.1128618; Oda T, 2007, J CELL BIOL, V177, P243, DOI 10.1083/jcb.200609038; OKELLY CJ, 1989, SYST ASSOC SPEC VOL, V38, P255; OKELLY CJ, 1984, PROTOPLASMA, V123, P18, DOI 10.1007/BF01283178; Provasoli L, 1968, CULTURES COLLECTIONS, P63; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; Rosenbaum JL, 2002, NAT REV MOL CELL BIO, V3, P813, DOI 10.1038/nrm952; Schoppmeier J, 2003, J PHYCOL, V39, P918, DOI 10.1046/j.1529-8817.2003.03031.x; TOTH R, 1973, NATURE, V243, P236, DOI 10.1038/243236a0; TOTH R, 1974, J PHYCOL, V10, P170; Ueki C, 2008, PHYCOLOGIA, V47, P5, DOI 10.2216/0031-8884(2008)47[5:ROTPPA]2.0.CO;2; VOROBJEV IA, 1987, INT REV CYTOL, V106, P227, DOI 10.1016/S0074-7696(08)61714-3	59	6	6	1	19	SPRINGER WIEN	WIEN	SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA	0033-183X	1615-6102		PROTOPLASMA	Protoplasma	FEB	2013	250	1					261	272		10.1007/s00709-012-0405-7			12	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	079QL	WOS:000314186400025	22476260				2021-04-07	
J	Wu, XM; Tronholm, A; Caceres, EF; Tovar-Corona, JM; Chen, L; Urrutia, AO; Hurst, LD				Wu, XianMing; Tronholm, Ana; Caceres, Eva Fernandez; Tovar-Corona, Jaime M.; Chen, Lu; Urrutia, Araxi O.; Hurst, Laurence D.			Evidence for Deep Phylogenetic Conservation of Exonic Splice-Related Constraints: Splice-Related Skews at Exonic Ends in the Brown Alga Ectocarpus Are Common and Resemble Those Seen in Humans	GENOME BIOLOGY AND EVOLUTION			English	Article						ESE; Ectocarpus; splicing; translational selection	SYNONYMOUS CODON USAGE; MESSENGER-RNA; DROSOPHILA-MELANOGASTER; NATURAL-SELECTION; HUMAN GENES; ENHANCERS; EVOLUTION; EUKARYOTES; COMPLEXITY; SEQUENCES	The control of RNA splicing is often modulated by exonic motifs near splice sites. Chief among these are exonic splice enhancers (ESEs). Well-described ESEs in mammals are purine rich and cause predictable skews in codon and amino acid usage toward exonic ends. Looking across species, those with relatively abundant intronic sequence are those with the more profound end of exon skews, indicative of exonization of splice site recognition. To date, the only intron-rich species that have been analyzed are mammals, precluding any conclusions about the likely ancestral condition. Here, we examine the patterns of codon and amino acid usage in the vicinity of exon-intron junctions in the brown alga Ectocarpus siliculosus, a species with abundant large introns, known SR proteins, and classical splice sites. We find that amino acids and codons preferred/avoided at both 30 and 50 ends in Ectocarpus, of which there are many, tend, on average, to also be preferred/avoided at the same exon ends in humans. Moreover, the preferences observed at the 50 ends of exons are largely the same as those at the 30 ends, a symmetry trend only previously observed in animals. We predict putative hexameric ESEs in Ectocarpus and show that these are purine rich and that there are many more of these identified as functional ESEs in humans than expected by chance. These results are consistent with deep phylogenetic conservation of SR protein binding motifs. Assuming codons preferred near boundaries are "splice optimal" codons, in Ectocarpus, unlike Drosophila, splice optimal and translationally optimal codons are not mutually exclusive. The exclusivity of translationally optimal and splice optimal codon sets is thus not universal.	[Chen, Lu] Wellcome Trust Sanger Inst, Hinxton, England		l.d.hurst@bath.ac.uk	Hurst, Laurence/F-9215-2010	Hurst, Laurence/0000-0002-1002-1054; Tronholm, Ana/0000-0002-0151-2804; Urrutia, Araxi/0000-0001-9011-8675	Royal SocietyRoyal Society of LondonEuropean Commission; CONACyT scholarshipConsejo Nacional de Ciencia y Tecnologia (CONACyT); University of Bath; Erasmus program	The authors thank Simon Dittami for advice on expression resources. A.O.U. is a Royal Society Dorothy Hodgkin Research Fellow and L. D. H. is a Royal Society Wolfsom Research Merit Award Holder. This work was supported by the a CONACyT scholarship (to J.M.T.-C.), the University of Bath (to X. W.), and the Erasmus program (to E.F.C.).	Adl SM, 2005, J EUKARYOT MICROBIOL, V52, P399, DOI 10.1111/j.1550-7408.2005.00053.x; AKASHI H, 1994, GENETICS, V136, P927; Blencowe BJ, 2000, TRENDS BIOCHEM SCI, V25, P106, DOI 10.1016/S0968-0004(00)01549-8; BOGUSKI MS, 1993, NAT GENET, V4, P332, DOI 10.1038/ng0893-332; Brett D, 2002, NAT GENET, V30, P29, DOI 10.1038/ng803; Carlini DB, 2006, J MOL EVOL, V62, P89, DOI 10.1007/s00239-005-0055-x; Cartegni L, 2002, NAT REV GENET, V3, P285, DOI 10.1038/nrg775; Chamary JV, 2005, TRENDS GENET, V21, P256, DOI 10.1016/j.tig.2005.03.001; Chen L, 2011, HUM MOL GENET, V20, P4422, DOI 10.1093/hmg/ddr370; Cock JM, 2012, ADV BOT RES, V64, P141, DOI 10.1016/B978-0-12-391499-6.00005-0; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Drummond DA, 2008, CELL, V134, P341, DOI 10.1016/j.cell.2008.05.042; Duret L, 2002, CURR OPIN GENET DEV, V12, P640, DOI 10.1016/S0959-437X(02)00353-2; Eddy SR, 1998, BIOINFORMATICS, V14, P755, DOI 10.1093/bioinformatics/14.9.755; Eskesen ST, 2004, GENETICS, V167, P543, DOI 10.1534/genetics.167.1.543; Eyre-Walker A, 2001, NAT REV GENET, V2, P549, DOI 10.1038/35080577; Fairbrother WG, 2004, PLOS BIOL, V2, P1388, DOI 10.1371/journal.pbio.0020268; Fairbrother WG, 2004, NUCLEIC ACIDS RES, V32, pW187, DOI 10.1093/nar/gkh393; Fairbrother WG, 2002, SCIENCE, V297, P1007, DOI 10.1126/science.1073774; GILBERT W, 1986, CELL, V46, P151, DOI 10.1016/0092-8674(86)90730-0; Goren A, 2006, MOL CELL, V22, P769, DOI 10.1016/j.molcel.2006.05.008; Graveley BR, 2000, RNA, V6, P1197, DOI 10.1017/S1355838200000960; Irimia M, 2007, TRENDS GENET, V23, P321, DOI 10.1016/j.tig.2007.04.001; Ke SD, 2011, GENOME RES, V21, P1360, DOI 10.1101/gr.119628.110; Kim E, 2007, NUCLEIC ACIDS RES, V35, P125, DOI 10.1093/nar/gkl924; Lim KH, 2011, P NATL ACAD SCI USA, V108, P11093, DOI 10.1073/pnas.1101135108; Logsdon JM, 1998, CURR OPIN GENET DEV, V8, P637, DOI 10.1016/S0959-437X(98)80031-2; Long JC, 2009, BIOCHEM J, V417, P15, DOI 10.1042/BJ20081501; Pan Q, 2008, NAT GENET, V40, P1413, DOI 10.1038/ng.259; Parmley JL, 2006, MOL BIOL EVOL, V23, P301, DOI 10.1093/molbev/msj035; Parmley JL, 2007, PLOS BIOL, V5, P343, DOI 10.1371/journal.pbio.0050014; Parmley JL, 2007, MOL BIOL EVOL, V24, P1600, DOI 10.1093/molbev/msm104; Plass M, 2008, TRENDS GENET, V24, P590, DOI 10.1016/j.tig.2008.10.004; Sharp PM, 2005, NUCLEIC ACIDS RES, V33, P1141, DOI 10.1093/nar/gki242; STOLTZFUS A, 1994, SCIENCE, V265, P202, DOI 10.1126/science.8023140; TANAKA K, 1994, MOL CELL BIOL, V14, P1347, DOI 10.1128/MCB.14.2.1347; Taniguchi I, 2007, P NATL ACAD SCI USA, V104, P13684, DOI 10.1073/pnas.0704922104; Warnecke T, 2007, MOL BIOL EVOL, V24, P2755, DOI 10.1093/molbev/msm210; Warnecke T, 2010, MOL SYST BIOL, V6, DOI 10.1038/msb.2009.94; Warnecke T, 2008, GENOME BIOL, V9, DOI 10.1186/gb-2008-9-2-r29; Webb CJ, 2005, GENE DEV, V19, P242, DOI 10.1101/gad.1265905; Willie E, 2004, TRENDS GENET, V20, P534, DOI 10.1016/j.tig.2004.08.014; Wu TD, 2005, BIOINFORMATICS, V21, P1859, DOI 10.1093/bioinformatics/bti310; Zhang Zhi-ping, 2009, Shengwu Jiagong Guocheng, V7, P23	45	5	6	0	5	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	1759-6653			GENOME BIOL EVOL	Genome Biol. Evol.		2013	5	9					1731	1745		10.1093/gbe/evt115			15	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	258CU	WOS:000327437600015	23902749	DOAJ Gold, Green Accepted, Green Published			2021-04-07	
J	Mahmood, N; Moosa, MM				Mahmood, Niaz; Moosa, Mahdi Muhammad			In silico analysis of the NBS protein family in Ectocarpus siliculosus	INDIAN JOURNAL OF BIOTECHNOLOGY			English	Article						Brown algae; Ectocarpus siliculosus; NBS; TPR	GENOME; GENES	Nucleotide-binding site (NBS) domain containing proteins belong to one of the most well characterized family of proteins; they are found in almost all higher eukaryotes. Extensive studies have been done on the plant NBS proteins, but similar studies on the brown algae NBS proteins are not available. In the present study, authors examined the diversity of NBS proteins in model brown algae, Ectocarpus siliculosus. A total of twenty six NBS proteins were identified and classified into different subfamilies based on their distinct domain organizations. Although many characteristics of the protein family are similar to those of plant species, several features are quite distinct. One such characteristic is the presence of tetratrico peptide repeat (TPR) motifs at the C-terminal ends of these proteins. Another interesting finding is the presence of two E. siliculosus specific conserved motifs leading to novel combination of the NBS domain. The remarkable structural diversity found among these proteins further strengthens the idea that diversifying selection may have played an important role in their evolution.	[Mahmood, Niaz; Moosa, Mahdi Muhammad] Univ Dhaka, Dept Biochem & Mol Biol, Mol Biol Lab, Dhaka 1000, Bangladesh; [Moosa, Mahdi Muhammad] Scripps Res Inst, Grad Program Biol Sci, La Jolla, CA 92037 USA	Mahmood, N (corresponding author), Univ Dhaka, Dept Biochem & Mol Biol, Mol Biol Lab, Dhaka 1000, Bangladesh.	niazmahmood.ami@gmail.com					Bailey T L, 1994, Proc Int Conf Intell Syst Mol Biol, V2, P28; Bailey TL, 2006, NUCLEIC ACIDS RES, V34, pW369, DOI 10.1093/nar/gkl198; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; D'Andrea LD, 2003, TRENDS BIOCHEM SCI, V28, P655, DOI 10.1016/j.tibs.2003.10.007; Dangl JL, 2001, NATURE, V411, P826, DOI 10.1038/35081161; Das AK, 1998, EMBO J, V17, P1192, DOI 10.1093/emboj/17.5.1192; DHALIWAL HS, 1999, PLANT BIOTECHNOL, V16, P255; Hagopian R, 2010, NUCLEIC ACIDS RES, V38, pW29, DOI 10.1093/nar/gkq298; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; LUPAS A, 1991, SCIENCE, V252, P1162, DOI 10.1126/science.252.5009.1162; Martin GB, 2003, ANNU REV PLANT BIOL, V54, P23, DOI 10.1146/annurev.arplant.54.031902.135035; Meyers BC, 2005, CURR OPIN PLANT BIOL, V8, P129, DOI 10.1016/j.pbi.2005.01.002; Meyers BC, 2003, PLANT CELL, V15, P809, DOI 10.1105/tpc.009308; Quevillon E, 2005, NUCLEIC ACIDS RES, V33, pW116, DOI 10.1093/nar/gki442; Richly E, 2002, MOL BIOL EVOL, V19, P76, DOI 10.1093/oxfordjournals.molbev.a003984; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Ting JPY, 2008, NAT REV IMMUNOL, V8, P372, DOI 10.1038/nri2296; TRAUT TW, 1994, EUR J BIOCHEM, V222, P9, DOI 10.1111/j.1432-1033.1994.tb18835.x; van der Biezen EA, 1998, CURR BIOL, V8, pR226, DOI 10.1016/S0960-9822(98)70145-9; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zhang JZ, 1998, P NATL ACAD SCI USA, V95, P3708, DOI 10.1073/pnas.95.7.3708; Zhou T, 2004, MOL GENET GENOMICS, V271, P402, DOI 10.1007/s00438-004-0990-z	23	1	1	0	0	NATL INST SCIENCE COMMUNICATION-NISCAIR	NEW DELHI	DR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIA	0972-5849	0975-0967		INDIAN J BIOTECHNOL	Indian J. Biotechnol.	JAN	2013	12	1			SI		98	102					5	Biotechnology & Applied Microbiology	Biotechnology & Applied Microbiology	138SY	WOS:000318531500012					2021-04-07	
J	Arun, A; Peters, NT; Scornet, D; Peters, AF; Cock, JM; Coelho, SM				Arun, Alok; Peters, Nick T.; Scornet, Delphine; Peters, Akira F.; Cock, J. Mark; Coelho, Susana M.			Non-cell autonomous regulation of life cycle transitions in the model brown alga Ectocarpus	NEW PHYTOLOGIST			English	Article						brown algae; development; Ectocarpus siliculosus; gametophyte; life cycle; reprogramming; sporophyte	SILICULOSUS; COMMUNICATION; CULTURES; GENOME; FATE	The model brown alga Ectocarpus has a haploid-diploid life cycle, involving alternation between two independent multicellular generations, the gametophyte and the sporophyte. Recent work has shown that alternation of generations is not determined by ploidy but is rather under genetic control, involving at least one master regulatory locus, OUROBOROS (ORO). Using cell biology approaches combined with measurements of generation-specific transcript abundance we provide evidence that alternation of generations can also be regulated by non-cell autonomous mechanisms. The Ectocarpus sporophyte produces a diffusible factor that causes major developmental reprogramming in gametophyte cells. Cells become resistant to reprogramming when the cell wall is synthetized, suggesting that the cell wall may play a role in locking an individual into the developmental program that has been engaged. A functional ORO gene is necessary for the induction of the developmental switch. Our results highlight the role of the cell wall in maintaining the differentiated generation stage once the appropriate developmental program has been engaged and also indicate that ORO is a key member of the developmental pathway triggered by the sporophyte factor. Alternation between gametophyte and sporophyte generations in Ectocarpus is surprisingly labile, perhaps reflecting an adaptation to the variable seashore environment inhabited by this alga.	[Arun, Alok; Peters, Nick T.; Scornet, Delphine; Peters, Akira F.; Cock, J. Mark; Coelho, Susana M.] CNRS, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, UMR 7139, F-29682 Roscoff, France; [Arun, Alok; Peters, Nick T.; Peters, Akira F.; Cock, J. Mark; Coelho, Susana M.] Univ Paris 06, Marine Plants & Biomol Lab, Stn Biol Roscoff, UMR 7139, F-29682 Roscoff, France; [Peters, Nick T.] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France	Coelho, SM (corresponding author), CNRS, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, UMR 7139, Pl Georges Teissier,BP74, F-29682 Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020; Arun, Alok/AAJ-7923-2020	Cock, J. Mark/0000-0002-2650-0383; Arun, Alok/0000-0003-4666-9802; Peters, Akira/0000-0001-5332-199X	Centre National de Recherche Scientifique; University Pierre and Marie Curie; Groupement d'Interet Scientifique Genomique Marine; Interreg program France (Channel)-England (project Marinexus); Agence Nationale de la Recherche (project Bi-cycle); EMBRC-France; European Erasmus Mundus program; IRES Fellowship	This project was supported by the Centre National de Recherche Scientifique, the University Pierre and Marie Curie, the Groupement d'Interet Scientifique Genomique Marine, the Interreg program France (Channel)-England (project Marinexus), the Agence Nationale de la Recherche (project Bi-cycle) and the EMBRC-France. A.A. was supported by a fellowship from the European Erasmus Mundus program. N.T.P. was supported by an IRES Fellowship.	BERGER F, 1994, SCIENCE, V263, P1421, DOI 10.1126/science.263.5152.1421; Bidle KD, 2011, CURR OPIN MICROBIOL, V14, P449, DOI 10.1016/j.mib.2011.07.013; Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Bouget FY, 1998, DEVELOPMENT, V125, P1999; Brownlee C, 2008, CURR BIOL, V18, pR518, DOI 10.1016/j.cub.2008.05.003; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2012, COLD SPRING HARBOR P, V2012, P361; Coelho SM, 2012, COLD SPRING HARBOR P, V2012, P262; Coelho SM, 2012, COLD SPRING HARBOR P, V2012, P258; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Hamant O, 2010, CURR OPIN GENET DEV, V20, P454, DOI 10.1016/j.gde.2010.04.009; Joint I, 2002, SCIENCE, V298, P1207, DOI 10.1126/science.1077075; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Muller DG, 2008, CAH BIOL MAR, V49, P59; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Pohnert G, 2007, TRENDS ECOL EVOL, V22, P198, DOI 10.1016/j.tree.2007.01.005; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x	22	15	15	2	28	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X			NEW PHYTOL	New Phytol.	JAN	2013	197	2					503	510		10.1111/nph.12007			8	Plant Sciences	Plant Sciences	067AY	WOS:000313265600020	23106314	Bronze			2021-04-07	
J	Cognat, V; Pawlak, G; Duchene, AM; Daujat, M; Gigant, A; Salinas, T; Michaud, M; Gutmann, B; Giege, P; Gobert, A; Marechal-Drouard, L				Cognat, Valerie; Pawlak, Gael; Duchene, Anne-Marie; Daujat, Magali; Gigant, Anais; Salinas, Thalia; Michaud, Morgane; Gutmann, Bernard; Giege, Philippe; Gobert, Anthony; Marechal-Drouard, Laurence			PlantRNA, a database for tRNAs of photosynthetic eukaryotes	NUCLEIC ACIDS RESEARCH			English	Article							GENOME SEQUENCE; GENES; COMPILATION; SYNTHETASES; EVOLUTION; IMPORT; ORIGIN	PlantRNA database (http://plantrna.ibmp.cnrs.fr/) compiles transfer RNA (tRNA) gene sequences retrieved from fully annotated plant nuclear, plastidial and mitochondrial genomes. The set of annotated tRNA gene sequences has been manually curated for maximum quality and confidence. The novelty of this database resides in the inclusion of biological information relevant to the function of all the tRNAs entered in the library. This includes 5'- and 3'-flanking sequences, A and B box sequences, region of transcription initiation and poly(T) transcription termination stretches, tRNA intron sequences, aminoacyl-tRNA synthetases and enzymes responsible for tRNA maturation and modification. Finally, data on mitochondrial import of nuclear-encoded tRNAs as well as the bibliome for the respective tRNAs and tRNA-binding proteins are also included. The current annotation concerns complete genomes from 11 organisms: five flowering plants (Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, Medicago truncatula and Brachypodium distachyon), a moss (Physcomitrella patens), two green algae (Chlamydomonas reinhardtii and Ostreococcus tauri), one glaucophyte (Cyanophora paradoxa), one brown alga (Ectocarpus siliculosus) and a pennate diatom (Phaeodactylum tricornutum). The database will be regularly updated and implemented with new plant genome annotations so as to provide extensive information on tRNA biology to the research community.	[Cognat, Valerie; Pawlak, Gael; Duchene, Anne-Marie; Daujat, Magali; Gigant, Anais; Salinas, Thalia; Michaud, Morgane; Gutmann, Bernard; Giege, Philippe; Gobert, Anthony; Marechal-Drouard, Laurence] Univ Strasbourg, CNRS, UPR 2357, Inst Biol Mol Plantes, F-67084 Strasbourg, France	Marechal-Drouard, L (corresponding author), Univ Strasbourg, CNRS, UPR 2357, Inst Biol Mol Plantes, 12 Rue Gen Zimmer, F-67084 Strasbourg, France.	laurence.drouard@ibmp-cnrs.unistra.fr		Gobert, Anthony/0000-0003-2626-8788; Giege, Philippe/0000-0003-2285-8190	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); University of Strasbourg; French Agence Nationale de la Recherche (ANR)French National Research Agency (ANR) [ANR-09-BLAN-0240-01, ANR-11-BSV8 008 01]; French National Program 'Investissement d'Avenir' (Labex MitoCross)French National Research Agency (ANR); French Ministere de l'Education et de la Recherche; CNRS, IBMP	Centre National de la Recherche Scientifique; University of Strasbourg; French Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0240-01, ANR-11-BSV8 008 01]; French National Program 'Investissement d'Avenir' (Labex MitoCross); University of Strasbourg and the French Ministere de l'Education et de la Recherche [to B. G. and M. M.]. Funding for open access charge: CNRS, IBMP.	Abe T, 2011, NUCLEIC ACIDS RES, V39, pD210, DOI 10.1093/nar/gkq1007; Banerjee R, 2010, FEBS LETT, V584, P387, DOI 10.1016/j.febslet.2009.11.013; Bowman JL, 2007, CELL, V129, P229, DOI 10.1016/j.cell.2007.04.004; Castellano S, 2004, EMBO REP, V5, P71, DOI 10.1038/sj.embor.7400036; Chan PP, 2009, NUCLEIC ACIDS RES, V37, pD93, DOI 10.1093/nar/gkn787; Chen P, 2010, BMC PLANT BIOL, V10, DOI 10.1186/1471-2229-10-201; Cognat V, 2008, GENETICS, V179, P113, DOI 10.1534/genetics.107.085688; Crooks GE, 2004, GENOME RES, V14, P1188, DOI 10.1101/gr.849004; Duchene AM, 2005, P NATL ACAD SCI USA, V102, P16484, DOI 10.1073/pnas.0504682102; Duchene AM, 2009, CURR GENET, V55, P1, DOI 10.1007/s00294-008-0223-9; Dudkiewicz M, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0032138; Emanuelsson O, 2007, NAT PROTOC, V2, P953, DOI 10.1038/nprot.2007.131; Geslain R, 2010, J MOL BIOL, V396, P821, DOI 10.1016/j.jmb.2009.12.018; Gobert A, 2010, NAT STRUCT MOL BIOL, V17, P740, DOI 10.1038/nsmb.1812; Goodstein DM, 2012, NUCLEIC ACIDS RES, V40, pD1178, DOI 10.1093/nar/gkr944; GREEN CJ, 1990, J BIOL CHEM, V265, P12139; Hurto RL, 2011, ADV EXP MED BIOL, V722, P137, DOI 10.1007/978-1-4614-0332-6_9; Jaillon O, 2007, NATURE, V449, P463, DOI 10.1038/nature06148; Janouskovec J, 2010, P NATL ACAD SCI USA, V107, P10949, DOI 10.1073/pnas.1003335107; Juhling F, 2009, NUCLEIC ACIDS RES, V37, pD159, DOI 10.1093/nar/gkn772; Kaul S, 2000, NATURE, V408, P796, DOI 10.1038/35048692; Lobanov AV, 2007, GENOME BIOL, V8, DOI 10.1186/gb-2007-8-9-r198; Marshall L, 2008, CELL, V133, P78, DOI 10.1016/j.cell.2008.02.035; Michaud M, 2011, PLANT J, V66, P80, DOI 10.1111/j.1365-313X.2011.04490.x; Phizicky EM, 2010, GENE DEV, V24, P1832, DOI 10.1101/gad.1956510; Rao M, 2003, RNA, V9, P923, DOI 10.1261/rna.5510503; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; Rudinger-Thirion J, 2011, P NATL ACAD SCI USA, V108, pE794, DOI 10.1073/pnas.1103698108; Salinas T, 2008, TRENDS BIOCHEM SCI, V33, P320, DOI 10.1016/j.tibs.2008.04.010; Schattner P, 2005, NUCLEIC ACIDS RES, V33, pW686, DOI 10.1093/nar/gki366; Schneider A, 2000, TRENDS CELL BIOL, V10, P509, DOI 10.1016/S0962-8924(00)01854-7; SCHNEIDER TD, 1990, NUCLEIC ACIDS RES, V18, P6097, DOI 10.1093/nar/18.20.6097; Small I, 2004, PROTEOMICS, V4, P1581, DOI 10.1002/pmic.200300776; Sprinzl M, 2005, NUCLEIC ACIDS RES, V33, pD139, DOI 10.1093/nar/gki012; Tuck AC, 2011, TRENDS GENET, V27, P422, DOI 10.1016/j.tig.2011.06.001; VANTOL H, 1988, NUCLEIC ACIDS RES, V16, P1951, DOI 10.1093/nar/16.5.1951; Velasco R, 2010, NAT GENET, V42, P833, DOI 10.1038/ng.654; Wu HJ, 2012, P NATL ACAD SCI USA, V109, P12219, DOI 10.1073/pnas.1209954109; Xu X, 2011, NATURE, V475, P189, DOI 10.1038/nature10158; Zhang G, 2011, NUCLEIC ACIDS RES, V39, P3331, DOI 10.1093/nar/gkq1257	40	38	37	0	25	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0305-1048	1362-4962		NUCLEIC ACIDS RES	Nucleic Acids Res.	JAN	2013	41	D1					D273	D279		10.1093/nar/gks935			7	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	062BE	WOS:000312893300039	23066098	DOAJ Gold, Green Published			2021-04-07	
J	Zambounis, A; Strittmatter, M; Gachon, CMM				Zambounis, Antonios; Strittmatter, Martina; Gachon, Claire M. M.			Chronic stress and disease resistance in the genome model marine seaweed Ectocarpus siliculosus	AQUATIC BOTANY			English	Article						Brown alga; Chronic stress; Ectocarpus siliculosus; Polyunsaturated fatty acid; Eurychasma dicksonii; Immunity; Induced resistance	REAL-TIME PCR; LAMINARIA-DIGITATA; EURYCHASMA-DICKSONII; OXIDATIVE BURST; DEFENSE; ALGAE; ACID; BIOSYNTHESIS; RESPONSES; IMMUNITY	In order to test the capacity of the genome model seaweed Ectocarpus siliculosus to acquire disease resistance, plantlets were repeatedly treated with the polyunsaturated fatty acids (PUFAs) linolenic and arachidonic acid in conditions known to increase the resistance of the kelp Laminaria digitata against the endophytic parasite Laminariocolax tomentosoides. Hydrogen peroxide, a well documented inducer of antioxidative defenses, was also applied as a positive control. Real-time PCR transcriptional profiling revealed an induction of a vanadium-bromoperoxidase, a heat-shock protein, a glutaredoxin and a glutathione S transferase, suggesting a transcriptional remodelling during chronic stress. We further assessed the resistance of E. siliculosus against the oomycete pathogen Eurychasma dicksonii following repeated exposure to arachidonic and linolenic acids. In contrast to observations made on L. digitata, we did not evidence any significant change in resistance compared to mock-treated control E. siliculosus. Altogether, our observations imply that E. siliculosus does react transcriptionally to chronic PUFA exposure. However, these inducible defenses may not be as potent as the ones of L. digitata, or they might be efficiently bypassed by Eu. dicksonii. (c) 2012 Elsevier B.V. All rights reserved.	[Zambounis, Antonios] Univ Thessaly, Sch Agr Sci, Nea Ionia 38446, Volos, Greece; [Zambounis, Antonios; Strittmatter, Martina; Gachon, Claire M. M.] Scottish Marine Inst, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland; [Zambounis, Antonios] Ctr Res & Technol Hellas CERTH, Inst Agrobiotechnol INA, GR-57001 Thessaloniki, Greece	Gachon, CMM (corresponding author), Scottish Marine Inst, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland.	cmmg@sams.ac.uk		Strittmatter, Martina/0000-0002-1258-9751; Gachon, Claire/0000-0002-3702-7472	Scottish Association for Marine Science; EU FP7 ASSEMBLE programme; ECOSUMMER Marie Curie PhD fellowship [MEST-CT-2005-20501]; Natural Environment Research Council SOFI initiative [NE/F012705/1]; Marie Curie Intra-European FellowshipEuropean Commission [MIEF-CT-2006-022837]; European Reintegration Grant from the European Commission [PERG03-GA-2008-230865]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [dml010007, NE/J00460X/1] Funding Source: researchfish	AZ gratefully acknowledges the support of a research bursary from the Scottish Association for Marine Science and a travel award of the EU FP7 ASSEMBLE programme. MS was the recipient of a ECOSUMMER Marie Curie PhD fellowship (MEST-CT-2005-20501). CMMG is funded by the Natural Environment Research Council SOFI initiative (award NE/F012705/1), a Marie Curie Intra-European Fellowship (MIEF-CT-2006-022837) and a European Reintegration Grant (PERG03-GA-2008-230865) from the European Commission.	ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Borell EM, 2004, OECOLOGIA, V140, P328, DOI 10.1007/s00442-004-1589-0; Bruce TJA, 2007, PLANT SCI, V173, P603, DOI 10.1016/j.plantsci.2007.09.002; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coleman RA, 2007, FUNCT ECOL, V21, P101, DOI 10.1111/j.1365-2435.2006.01210.x; Contreras L, 2009, AQUAT TOXICOL, V94, P94, DOI 10.1016/j.aquatox.2009.06.004; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Gachon C, 2004, PLANT PHYSIOL BIOCH, V42, P367, DOI 10.1016/j.plaphy.2004.04.001; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Galis I, 2009, PLANT CELL ENVIRON, V32, P617, DOI 10.1111/j.1365-3040.2008.01862.x; Goulitquer S, 2009, CHEMBIOCHEM, V10, P977, DOI 10.1002/cbic.200900004; Grenville-Briggs L, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0024500; Haavisto F, 2010, OECOLOGIA, V162, P685, DOI 10.1007/s00442-009-1494-7; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Kupper FC, 2009, PLANT CELL PHYSIOL, V50, P789, DOI 10.1093/pcp/pcp023; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Meyer Y, 2009, ANNU REV GENET, V43, P335, DOI 10.1146/annurev-genet-102108-134201; Mosblech A, 2009, PLANT PHYSIOL BIOCH, V47, P511, DOI 10.1016/j.plaphy.2008.12.011; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; O'Connell RJ, 2006, NEW PHYTOL, V171, P699, DOI 10.1111/j.1469-8137.2006.01829.x; Oztetik E, 2008, BOT REV, V74, P419, DOI 10.1007/s12229-008-9013-9; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Pfaffl MW, 2002, NUCLEIC ACIDS RES, V30, DOI 10.1093/nar/30.9.e36; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Richter K, 2010, MOL CELL, V40, P253, DOI 10.1016/j.molcel.2010.10.006; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Rui F, 2010, J ORG CHEM, V75, P3958, DOI 10.1021/jo1004372; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Spoel SH, 2008, CELL HOST MICROBE, V3, P348, DOI 10.1016/j.chom.2008.05.009; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Thomas F, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0021475; Thomma BPHJ, 2001, CURR OPIN IMMUNOL, V13, P63, DOI 10.1016/S0952-7915(00)00183-7; Verhage A, 2010, PLANT PHYSIOL, V154, P536, DOI 10.1104/pp.110.161570; Wang Y, 2006, J ENVIRON SCI, V18, P543; Weinberger F, 2007, BIOL BULL-US, V213, P290, DOI 10.2307/25066646; Wiesemeier T, 2008, J CHEM ECOL, V34, P1523, DOI 10.1007/s10886-008-9568-2	42	8	8	1	42	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0304-3770	1879-1522		AQUAT BOT	Aquat. Bot.	JAN	2013	104						147	152		10.1016/j.aquabot.2012.07.008			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	070AR	WOS:000313478700018					2021-04-07	
J	Evariste, E; Gatley, CM; Detty, MR; Callow, ME; Callow, JA				Evariste, Emmanuelle; Gatley, Caitlyn M.; Detty, Michael R.; Callow, Maureen E.; Callow, James A.			The performance of aminoalkyl/fluorocarbon/hydrocarbon-modified xerogel coatings against the marine alga Ectocarpus crouaniorum: relative roles of surface energy and charge	BIOFOULING			English	Article						marine biofouling; xerogels; brown algae; Ectocarpus crouaniorum; Ulva linza; fouling-release; surface energy; charge	CONTACT-ANGLE HYSTERESIS; FOULING-RELEASE; SETTLEMENT BEHAVIOR; ADHESION STRENGTH; ULVA; SHIP; POLYSACCHARIDES; EXTRACTION; SPORELINGS; COPOLYMERS	The effect of a series of xerogel coatings modified with aminoalkyl/fluorocarbon/hydrocarbon groups on the adhesion of a new test species, the filamentous brown alga Ectocarpus crouaniorum, has been explored, and compared with the green alga Ulva linza. The results showed that E. crouaniorum adhered weakly to the less polar, low wettability coatings in the series, but stronger adhesion was shown on polar, higher surface energy coatings containing aminoalkyl groups. The results from a separate series of coatings tuned to have similar surface energies and polarities after immersion in artificial seawater (ASW), but widely different surface charges, demonstrated that surface charge was more important than surface energy and polarity in determining the adhesion strength of both E. crouaniorum and U. linza on xerogel coatings. No correlation was found between adhesion and contact angle hysteresis. X-ray photoelectron spectroscopy analysis of samples after immersion in ASW confirmed the presence of charged ammonium groups on the surface of the aminoalkylated coatings.	[Evariste, Emmanuelle; Callow, Maureen E.; Callow, James A.] Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England; [Gatley, Caitlyn M.; Detty, Michael R.] SUNY Coll Buffalo, Dept Chem, Buffalo, NY 14222 USA	Callow, JA (corresponding author), Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England.	j.a.callow@bham.ac.uk			International Paint Ltd; Office of Naval ResearchOffice of Naval Research [N00014-08-1-0010, N0014-09-1-0217]	E. Evariste thanks International Paint Ltd for a Studentship supporting these studies. JAC/MEC and MRD thank the Office of Naval Research (awards N00014-08-1-0010 and N0014-09-1-0217 respectively) for partial support of these studies. CMG and MRD thank Jill Fornalik (SUNY Buffalo) for her assistance in acquiring AFM data for surface roughness and Young's modulus.	Aldred N, 2011, ACS APPL MATER INTER, V3, P2085, DOI 10.1021/am2003075; Baier RE, 1992, BIOFOULING, V6, P165, DOI 10.1080/08927019209386220; Bennett SM, 2010, BIOFOULING, V26, P235, DOI 10.1080/08927010903469676; Brinker C. J., 1990, SOL GEL SCI PHYS CHE; Callow JA, 2011, NAT COMMUN, V2, DOI 10.1038/ncomms1251; Cavalier-Smith T, 2004, P ROY SOC B-BIOL SCI, V271, P1251, DOI 10.1098/rspb.2004.2705; Chaudhury MK, 2005, BIOFOULING, V21, P41, DOI 10.1080/08927010500044377; Cooper SP, 2011, BIOFOULING, V27, P881, DOI 10.1080/08927014.2011.611305; Ederth T, 2008, BIOFOULING, V24, P303, DOI 10.1080/08927010802192650; Ederth T, 2009, LANGMUIR, V25, P9375, DOI 10.1021/la900688g; Evariste E, 2012, BIOFOULING, V28, P15, DOI 10.1080/08927014.2011.643466; Finlay JA, 2008, BIOFOULING, V24, P219, DOI 10.1080/08927010802040693; Finlay JA, 2010, BIOFOULING, V26, P657, DOI 10.1080/08927014.2010.506242; Finnie A.A., 2010, BIOFOULING, P185, DOI 10.1002/97814 44315462.ch13.; Fletcher R. L., 1980, CATALOGUE MAIN MARIN, V6; Gunari N, 2011, BIOFOULING, V27, P137, DOI 10.1080/08927014.2010.548599; HALL HK, 1957, J AM CHEM SOC, V79, P5441, DOI 10.1021/ja01577a030; JEFFREY SW, 1975, BIOCHEM PHYSIOL PFL, V167, P191, DOI 10.1016/s0015-3796(17)30778-3; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Martinelli E, 2008, LANGMUIR, V24, P13138, DOI 10.1021/la801991k; McMaster D, 2009, BIOFOULING, V25, P21, DOI 10.1080/08927010802431298; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Mineur F, 2007, MAR BIOL, V151, P1299, DOI 10.1007/s00227-006-0567-y; OWENS DK, 1969, J APPL POLYM SCI, V13, P1741, DOI 10.1002/app.1969.070130815; Pandey S, 2000, CHEM MATER, V12, P3547, DOI 10.1021/cm000580z; Park D, 2010, ACS APPL MATER INTER, V2, P703, DOI 10.1021/am900748v; Petrone L, 2011, J R SOC INTERFACE, V8, P410, DOI 10.1098/rsif.2010.0316; Petrone L, 2011, BIOFOULING, V27, P1043, DOI 10.1080/08927014.2011.625474; Potin P, 2006, BIOLOGICAL ADHESIVES, P105, DOI 10.1007/978-3-540-31049-5_6; Quinn G.P., 2002, EXPT DESIGN DATA ANA; RAY B, 1995, CARBOHYD RES, V274, P251, DOI 10.1016/0008-6215(95)00138-J; Schmidt DL, 2004, LANGMUIR, V20, P2830, DOI 10.1021/la035385o; Schultz MP, 2011, BIOFOULING, V27, P87, DOI 10.1080/08927014.2010.542809; Schultz MP, 2007, BIOFOULING, V23, P331, DOI 10.1080/08927010701461974; Schultz MP, 2000, BIOFOULING, V15, P243, DOI 10.1080/08927010009386315; Schultz MP, 2003, BIOFOULING, V19, P17, DOI 10.1080/0892701031000089516; Selvaggio P, 2009, INT J NAUT ARCHAEOL, V38, P417, DOI 10.1111/j.1095-9270.2009.00241.x; SHOAF WT, 1976, LIMNOL OCEANOGR, V21, P926, DOI 10.4319/lo.1976.21.6.0926; Sokolova A, 2012, BIOFOULING, V28, P143, DOI 10.1080/08927014.2012.659244; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Tang Y, 2005, BIOFOULING, V21, P59, DOI 10.1080/08927010500070935; Tribou M, 2010, BIOFOULING, V26, P47, DOI 10.1080/08927010903290973; Ucar IO, 2010, BIOINTERPHASES, V5, P75, DOI 10.1116/1.3483467	43	12	12	0	46	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0892-7014	1029-2454		BIOFOULING	Biofouling		2013	29	2					171	184		10.1080/08927014.2012.758717			14	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	073HA	WOS:000313732900006	23330687				2021-04-07	
J	Mahmood, N; Moosa, MM; Tamanna, N; Sarker, SK; Najnin, RA; Alam, SS				Mahmood, Niaz; Moosa, Mahdi Muhammad; Tamanna, Nahid; Sarker, Suprovath Kumar; Najnin, Rifat Ara; Alam, Salma Sultana			In silico Analysis Reveals the Presence of a Large Number of Ankyrin Repeat Containing Proteins in Ectocarpus siliculosus	INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES			English	Article						Ankyrin repeat; Ectocarpus siliculosus; brown algae	GENES	Proteins with Ankyrin repeat motifs (ANK) are found to be associated with diverse biological processes and molecular functions in most of the studied organisms. Several studies have been done on the ANK-motif containing proteins of various model species, but similar studies on their counterparts in brown algae are not available. In this study, we have identified a total of 1,372 ankyrin repeats in 339 proteins of the model brown algae Ectocarpus siliculosus and the consensus sequence of the ANK repeats was determined. The proteins were classified into eight different subfamilies depending on their structural diversity. The data provided in this study may provide useful basis for future reverse genetics analysis of the members of this family.	[Mahmood, Niaz; Moosa, Mahdi Muhammad; Sarker, Suprovath Kumar; Najnin, Rifat Ara; Alam, Salma Sultana] Univ Dhaka, Dept Biochem & Mol Biol, Mol Biol Lab, Dhaka 1000, Bangladesh; [Tamanna, Nahid] Univ Manitoba, Grad Program Biol Sci, Winnipeg, MB R3E 0J9, Canada; [Mahmood, Niaz] Univ Manitoba, Grad Program Biochem & Med Genet, Winnipeg, MB R3T 2N2, Canada; [Moosa, Mahdi Muhammad] Scripps Res Inst, Grad Program Biol Sci, La Jolla, CA 92037 USA	Mahmood, N (corresponding author), Univ Dhaka, Dept Biochem & Mol Biol, Mol Biol Lab, Dhaka 1000, Bangladesh.	niazmahmood.ami@gmail.com					Bailey TL, 2006, NUCLEIC ACIDS RES, V34, pW369, DOI 10.1093/nar/gkl198; Becerra C, 2004, GENE, V340, P111, DOI 10.1016/j.gene.2004.06.006; BREEDEN L, 1987, NATURE, V329, P651, DOI 10.1038/329651a0; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Crooks GE, 2004, GENOME RES, V14, P1188, DOI 10.1101/gr.849004; Huang JY, 2009, PLANT MOL BIOL, V71, P207, DOI 10.1007/s11103-009-9518-6; Jebanathirajah JA, 2002, TRENDS PLANT SCI, V7, P388, DOI 10.1016/S1360-1385(02)02309-9; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; Li JN, 2006, BIOCHEMISTRY-US, V45, P15168, DOI 10.1021/bi062188q; Mahmood N., 2012, INTERDISCIP BIOCENTR, V4, P1; Marcotte EM, 1999, J MOL BIOL, V293, P151, DOI 10.1006/jmbi.1999.3136; Mosavi LK, 2004, PROTEIN SCI, V13, P1435, DOI 10.1110/ps.03554604; Mosavi LK, 2002, P NATL ACAD SCI USA, V99, P16029, DOI 10.1073/pnas.252537899; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Sedgwick SG, 1999, TRENDS BIOCHEM SCI, V24, P311, DOI 10.1016/S0968-0004(99)01426-7; Seong ES, 2007, J BIOCHEM MOL BIOL, V40, P952; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Wang YS, 2006, PLANT CELL, V18, P3635, DOI 10.1105/tpc.106.046730; Yan JQ, 2002, PLANT J, V29, P193, DOI 10.1046/j.0960-7412.2001.01205.x; ZHANG H, 1992, PLANT CELL, V4, P1575, DOI 10.1105/tpc.4.12.1575	20	1	1	0	6	SPRINGER HEIDELBERG	HEIDELBERG	TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY	1913-2751	1867-1462		INTERDISCIP SCI	Interdiscip. Sci.	DEC	2012	4	4					291	295		10.1007/s12539-012-0134-9			5	Mathematical & Computational Biology	Mathematical & Computational Biology	145WU	WOS:000319050900006	23354818				2021-04-07	
J	Draisma, SGA; Eurlings, MCM; Lim, PE				Draisma, Stefano G. A.; Eurlings, Marcel C. M.; Lim, Phaik-Eem			High intra-individual sequence variation in the nuclear rDNA LSU-5S intergenic spacer in the Sargassaceae (Fucales, Phaeophyceae)	JOURNAL OF APPLIED PHYCOLOGY			English	Article						Alignment-free analysis; Intra-individual variation; Linked 5S; rDNA intergenic spacer; Pseudogenic; Sargassaceae; Tandem repeats	5S RIBOSOMAL-RNA; BROWN ALGAL GENERA; MOLECULAR PHYLOGENY; TAXONOMIC REVISION; GENETIC DIVERSITY; DNA-SEQUENCES; ECTOCARPUS ECTOCARPALES; NORTHERN CHILE; WHOLE GENOMES; LIFE-HISTORY	Of four species of Sargassaceae, representing the genera Carpophyllum, Cystoseira, Landsburgia, and Sargassum, the intergenic spacer of the ribosomal cistron was amplified using a forward primer annealing at the 3'-end of the large subunit (LSU) of the ribosomal cistron and a reverse primer annealing at the 5S rRNA gene. The PCR products were cloned and the DNA sequences of multiple clones were determined. Almost each clone showed a unique DNA sequence. Intra-individual variation of this LSU-5S intergenic spacer was extremely high and was characterized by great length variation and a high number of short tandem repeats. Sequences were unalignable and therefore it was concluded that the LSU-5S intergenic spacer is unsuitable for phylogenetic and phylogeographic studies of sargassacean taxa.	[Draisma, Stefano G. A.; Lim, Phaik-Eem] Univ Malaya, Inst Ocean & Earth Sci, Kuala Lumpur 50603, Malaysia; [Eurlings, Marcel C. M.] Leiden Univ, Netherlands Ctr Biodivers Nat, Sect NHN, NL-2300 RA Leiden, Netherlands; [Lim, Phaik-Eem] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia	Draisma, SGA (corresponding author), Univ Malaya, Inst Ocean & Earth Sci, Kuala Lumpur 50603, Malaysia.	sgadraisma@yahoo.com	Draisma, Stefano/B-5391-2010; LIM, PHAIK EEM/B-5331-2010	Draisma, Stefano/0000-0002-0446-908X; LIM, PHAIK EEM/0000-0001-9186-1487	US National Science FoundationNational Science Foundation (NSF) [DEB 0629564]	Christian Bodeker, Ignacio Barbara, and Lynne McIvor are thanked for collecting the sargassacean specimens used in this study. Laboratory costs were supported in part by a grant from the US National Science Foundation (DEB 0629564).	Benson G, 1999, NUCLEIC ACIDS RES, V27, P573, DOI 10.1093/nar/27.2.573; Burkhardt E, 1998, J PHYCOL, V34, P682, DOI 10.1046/j.1529-8817.1998.340682.x; Camus C, 2005, J PHYCOL, V41, P931, DOI 10.1111/j.1529-8817.2005.00121.x; Cheang CC, 2010, J PHYCOL, V46, P1063, DOI 10.1111/j.1529-8817.2010.00901.x; Cheang CC, 2010, MOL ECOL, V19, P2933, DOI 10.1111/j.1365-294X.2010.04685.x; Cho GY, 2007, PHYCOLOGIA, V46, P657, DOI 10.2216/06-70.1; Cho GY, 2005, PHYCOLOGIA, V44, P103, DOI 10.2216/0031-8884(2005)44[103:GPAPOC]2.0.CO;2; Contreras L, 2007, J PHYCOL, V43, P1320, DOI 10.1111/j.1529-8817.2007.00413.x; Coyer JA, 2001, J PHYCOL, V37, P574, DOI 10.1046/j.1529-8817.2001.037001574.x; Draisma SGA, 2010, J PHYCOL, V46, P1329, DOI 10.1111/j.1529-8817.2010.00891.x; Ercegovic A., 1959, INT REV GES HYDROBIO, V44, P32; Erting L, 2004, EUR J PHYCOL, V39, P243, DOI 10.1080/09670260410001712563; Fama P, 2000, EUR J PHYCOL, V35, P349, DOI 10.1017/S0967026200002948; Gile GH, 2010, J PHYCOL, V46, P743, DOI 10.1111/j.1529-8817.2010.00851.x; Harvey JBJ, 2006, J PHYCOL, V42, P707, DOI 10.1111/j.1529-8817.2006.00215.x; Henkel SK, 2007, BOT MAR, V50, P159, DOI 10.1515/BOT.2007.019; Jiang HX, 2009, J PHYCOL, V45, P1270, DOI 10.1111/j.1529-8817.2009.00755.x; Kain JM, 2010, PHYCOLOGIA, V49, P617, DOI 10.2216/09-96.1; Kawai H, 2000, PHYCOLOGIA, V39, P416, DOI 10.2216/i0031-8884-39-5-416.1; Kawai H, 2004, J PHYCOL, V40, P1156, DOI 10.1111/j.1529-8817.2004.03153.x; KAWAI H, 1995, J PHYCOL, V31, P306, DOI 10.1111/j.0022-3646.1995.00306.x; Kim SH, 2003, PHYCOLOGIA, V42, P183, DOI 10.2216/i0031-8884-42-2-183.1; Kim SH, 2002, PHYCOLOGIA, V41, P328, DOI 10.2216/i0031-8884-41-4-328.1; Kogame K, 2001, CRYPTOGAMIE ALGOL, V22, P201, DOI 10.1016/S0181-1568(01)01064-9; Kraan S, 2000, J APPL PHYCOL, V12, P577, DOI 10.1023/A:1026519030398; Kucera H, 2008, BOTANY, V86, P1065, DOI 10.1139/B08-056; Lane CE, 2007, MOL PHYLOGENET EVOL, V44, P634, DOI 10.1016/j.ympev.2007.03.016; Leclerc MC, 1998, J MOL EVOL, V46, P115, DOI 10.1007/PL00006278; LIM BL, 1986, JPN J GENET, V61, P169, DOI 10.1266/jjg.61.169; Mattio L, 2010, CRYPTOGAMIE ALGOL, V31, P467; Mattio L, 2009, J PHYCOL, V45, P1213, DOI 10.1111/j.1529-8817.2009.00737.x; Mayer C, 2006, PHOBOS 3 3 11; Miller KA, 2000, J PHYCOL, V36, P862, DOI 10.1046/j.1529-8817.2000.99233.x; Ni-Ni-Win, 2011, EUR J PHYCOL, V46, P327, DOI 10.1080/09670262.2011.614355; Peters AF, 2000, POLAR BIOL, V23, P95, DOI 10.1007/s003000050013; Peters AF, 1997, J PHYCOL, V33, P294, DOI 10.1111/j.0022-3646.1997.00294.x; Peters AF, 1998, PHYCOLOGIA, V37, P106, DOI 10.2216/i0031-8884-37-2-106.1; Peters AF, 1998, EUR J PHYCOL, V33, P65, DOI 10.1080/09670269810001736543; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Qi J, 2004, NUCLEIC ACIDS RES, V32, pW45, DOI 10.1093/nar/gkh362; Qi J, 2004, J MOL EVOL, V58, P1, DOI 10.1007/s00239-003-2493-7; Roberts M., 1978, MODERN APPROACHES TA, P399; Ruggiero MV, 2004, J MOL EVOL, V58, P115, DOI 10.1007/s00239-003-2536-0; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Sasaki H, 2007, PHYCOLOGIA, V46, P10, DOI 10.2216/06-06.1; Serrao EA, 1999, J PHYCOL, V35, P382, DOI 10.1046/j.1529-8817.1999.3520382.x; Shi CJ, 2005, J INTEGR PLANT BIOL, V47, P283, DOI 10.1111/j.1744-7909.2005.00036.x; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Soltis Douglas E., 1998, P1; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Uwai S, 2006, HYDROBIOLOGIA, V553, P345, DOI 10.1007/s10750-005-0883-0; Xu Z, 2009, NUCLEIC ACIDS RES, V37, pW174, DOI 10.1093/nar/gkp278; Yotsukura N, 2002, J APPL PHYCOL, V14, P233, DOI 10.1023/A:1021166218681; Yotsukura N, 2006, PHYCOL RES, V54, P269, DOI 10.1111/j.1440-1835.2006.00434.x	55	5	6	1	21	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0921-8971			J APPL PHYCOL	J. Appl. Phycol.	DEC	2012	24	6					1373	1379		10.1007/s10811-012-9789-1			7	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	025YF	WOS:000310232900004					2021-04-07	
J	Iyer, LM; Aravind, L				Iyer, Lakshminarayan M.; Aravind, L.			ALOG domains: provenance of plant homeotic and developmental regulators from the DNA-binding domain of a novel class of DIRS1-type retroposons	BIOLOGY DIRECT			English	Article						DIRS1; Tyrosine recombinase; Plant development; DNA-binding; Retroposon; Transcription factor; Chromatin protein; Plant defense	TRANSCRIPTION FACTORS; STRUCTURAL BASIS; NATURAL-HISTORY; GENE FAMILY; PROTEIN; RECOMBINATION; ALIGNMENT; GENOMICS	Members of the Arabidopsis LSH1 and Oryza G1 (ALOG) family of proteins have been shown to function as key developmental regulators in land plants. However, their precise mode of action remains unclear. Using sensitive sequence and structure analysis, we show that the ALOG domains are a distinct version of the N-terminal DNA-binding domain shared by the XerC/D-like, protelomerase, topoisomerase-IA, and Flp tyrosine recombinases. ALOG domains are distinguished by the insertion of an additional zinc ribbon into this DNA-binding domain. In particular, we show that the ALOG domain is derived from the XerC/D-like recombinases of a novel class of DIRS-1-like retroposons. Copies of this element, which have been recently inactivated, are present in several marine metazoan lineages, whereas the stramenopile Ectocarpus, retains an active copy of the same. Thus, we predict that ALOG domains help establish organ identity and differentiation by binding specific DNA sequences and acting as transcription factors or recruiters of repressive chromatin. They are also found in certain plant defense proteins, where they are predicted to function as DNA sensors. The evolutionary history of the ALOG domain represents a unique instance of a domain, otherwise exclusively found in retroelements, being recruited as a specific transcription factor in the streptophyte lineage of plants. Hence, they add to the growing evidence for derivation of DNA-binding domains of eukaryotic specific TFs from mobile and selfish elements.	[Iyer, Lakshminarayan M.; Aravind, L.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA	Aravind, L (corresponding author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA.	aravind@ncbi.nlm.nih.gov	Iyer, Lakshminarayan M./H-2996-2019	Aravind, L/0000-0003-0771-253X	US Department of Health and Human Services (National Library of Medicine, NIH)United States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Library of Medicine (NLM); NATIONAL LIBRARY OF MEDICINEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Library of Medicine (NLM) [ZIALM594244, ZIALM594244, ZIALM594244, ZIALM594244, ZIALM594244, ZIALM594244, ZIALM594244, ZIALM594244, ZIALM594244, ZIALM594244] Funding Source: NIH RePORTER	The authors' research is supported by the intramural funds of the US Department of Health and Human Services (National Library of Medicine, NIH). We thank Dr. R. F. de Souza for writing a script that was useful for this analysis. This article was reviewed by Valerian Dolja and Gaspar Jekely. Additional file 1 can also be accessed from ftp://ftp.ncbi.nih.gov/pub/aravind/ALOG/ALOG.html	Aihara H, 2007, MOL CELL, V27, P901, DOI 10.1016/j.molcel.2007.07.026; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Aravind L, 2000, TRENDS BIOCHEM SCI, V25, P421, DOI 10.1016/S0968-0004(00)01620-0; Aravind L, 2005, FEMS MICROBIOL REV, V29, P231, DOI 10.1016/j.fmrre.2004.12.008; Aravind L., 2002, GENOME BIOL, V3; Babu MM, 2006, NUCLEIC ACIDS RES, V34, P6505, DOI 10.1093/nar/gkl888; Balaji S, 2005, NUCLEIC ACIDS RES, V33, P3994, DOI 10.1093/nar/gki709; Biswas T, 2005, NATURE, V435, P1059, DOI 10.1038/nature03657; Charoensawan V, 2010, TRENDS GENET, V26, P388, DOI 10.1016/j.tig.2010.06.004; Chen Y, 2000, MOL CELL, V6, P885, DOI 10.1016/S1097-2765(05)00088-2; Cole C, 2008, NUCLEIC ACIDS RES, V36, pW197, DOI 10.1093/nar/gkn238; Eddy Sean R, 2009, Genome Inform, V23, P205; Edgar RC, 2004, BMC BIOINFORMATICS, V5, P1, DOI 10.1186/1471-2105-5-113; Finn RD, 2010, NUCLEIC ACIDS RES, V38, pD211, DOI 10.1093/nar/gkp985; Goodwin TJD, 2004, MOL BIOL EVOL, V21, P746, DOI 10.1093/molbev/msh072; Goodwin TJD, 2003, MICROBIOL-SGM, V149, P3099, DOI 10.1099/mic.0.26529-0; Guo F, 1997, NATURE, V389, P40, DOI 10.1038/37925; Holm L, 2008, BIOINFORMATICS, V24, P2780, DOI 10.1093/bioinformatics/btn507; Iyer LA, 2006, VIRUS RES, V117, P156, DOI 10.1016/j.virusres.2006.01.009; Iyer LM, 2008, INT J PARASITOL, V38, P1, DOI 10.1016/j.ijpara.2007.07.018; Iyer LM, 2012, J STRUCT BIOL, V179, P299, DOI 10.1016/j.jsb.2011.12.013; Iyer LM, 2011, PROG MOL BIOL TRANSL, V101, P25, DOI 10.1016/B978-0-12-387685-0.00002-0; Izsvak Z, 2000, J MOL BIOL, V302, P93, DOI 10.1006/jmbi.2000.4047; Jun SH, 2011, CRIT REV BIOCHEM MOL, V46, P27, DOI 10.3109/10409238.2010.538662; Lassmann T, 2009, NUCLEIC ACIDS RES, V37, P858, DOI 10.1093/nar/gkn1006; Poulter RTM, 2005, CYTOGENET GENOME RES, V110, P575, DOI 10.1159/000084991; Price MN, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0009490; Redinbo MR, 1999, J MOL BIOL, V292, P685, DOI 10.1006/jmbi.1999.3065; Roussigne M, 2003, TRENDS BIOCHEM SCI, V28, P66, DOI 10.1016/S0968-0004(02)00013-0; Schumacher MA, 2003, CELL, V115, P413, DOI 10.1016/S0092-8674(03)00887-0; Siegmund T, 2002, DEV GENES EVOL, V212, P152, DOI 10.1007/s00427-002-0219-2; Smit AFA, 1996, P NATL ACAD SCI USA, V93, P1443, DOI 10.1073/pnas.93.4.1443; Soding J, 2005, NUCLEIC ACIDS RES, V33, pW244, DOI 10.1093/nar/gki408; Takeda S, 2011, PLANT J, V66, P1066, DOI 10.1111/j.1365-313X.2011.04571.x; Watson J. D, 2008, MOL BIOL GENE; Wessler SR, 2005, TRENDS PLANT SCI, V10, P54, DOI 10.1016/j.tplants.2004.12.007; Yamasaki K, 2004, PLANT CELL, V16, P3448, DOI 10.1105/tpc.104.026112; Yoshida A, 2009, P NATL ACAD SCI USA, V106, P20103, DOI 10.1073/pnas.0907896106; Zhao L, 2004, PLANT J, V37, P694, DOI 10.1111/j.1365-313X.2003.01993.x	39	25	47	1	10	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND		1745-6150		BIOL DIRECT	Biol. Direct	NOV 12	2012	7								39	10.1186/1745-6150-7-39			8	Biology	Life Sciences & Biomedicine - Other Topics	100TO	WOS:000315726700001	23146749	DOAJ Gold, Green Published			2021-04-07	
J	Bottger, LH; Miller, EP; Andresen, C; Matzanke, BF; Kupper, FC; Carrano, CJ				Boettger, Lars H.; Miller, Eric P.; Andresen, Christian; Matzanke, Berthold F.; Kuepper, Frithjof C.; Carrano, Carl J.			Atypical iron storage in marine brown algae: a multidisciplinary study of iron transport and storage in Ectocarpus siliculosus	JOURNAL OF EXPERIMENTAL BOTANY			English	Article						Ectocarpus; iron; marine algae; M; ssbauer spectroscopy; storage; transport; X-ray absorbtion spectroscopy	FERRIC REDUCTASE-ACTIVITY; FERROUS IRON; METHANOBACTERIUM-THERMOAUTOTROPHICUM; CHLAMYDOMONAS-REINHARDTII; MOSSBAUER-SPECTROSCOPY; ESCHERICHIA-COLI; PLASMA-MEMBRANE; PHYTOPLANKTON; ACQUISITION; MODEL	Iron is an essential element for all living organisms due to its ubiquitous role in redox and other enzymes, especially in the context of respiration and photosynthesis. The iron uptake and storage systems of terrestrial/higher plants are now reasonably well understood, with two basic strategies for iron uptake being distinguished: strategy I plants use a mechanism involving induction of Fe(III)-chelate reductase (ferrireductase) and Fe(II) transporter proteins, while strategy II plants utilize high-affinity, iron-specific, binding compounds called phytosiderophores. In contrast, little is known about the corresponding systems in marine, plant-like lineages, particularly those of multicellular algae (seaweeds). Herein the first study of the iron uptake and storage mechanisms in the brown alga Ectocarpus siliculosus is reported. Genomic data suggest that Ectocarpus may use a strategy I approach. Short-term radio-iron uptake studies verified that iron is taken up by Ectocarpus in a time- and concentration-dependent manner consistent with an active transport process. Upon long-term exposure to Fe-57, two metabolites have been identified using a combination of Mssbauer and X-ray absorption spectroscopies. These include an ironsulphur cluster accounting for similar to 26% of the total intracellular iron pool and a second component with spectra typical of a polymeric (Fe3+O6) system with parameters similar to the amorphous phosphorus-rich mineral core of bacterial and plant ferritins. This iron metabolite accounts for similar to 74% of the cellular iron pool and suggests that Ectocarpus contains a non-ferritin but mineral-based iron storage pool.	[Boettger, Lars H.; Andresen, Christian; Matzanke, Berthold F.] Med Univ Lubeck, Isotopes Lab, Sect Nat Sci, D-23538 Lubeck, Germany; [Miller, Eric P.; Carrano, Carl J.] San Diego State Univ, Dept Chem & Biochem, San Diego, CA 92182 USA; [Kuepper, Frithjof C.] Scottish Marine Inst, Scottish Assoc Marine Sci, Microbial & Mol Biol Dept, Oban, Argyll, Scotland	Matzanke, BF (corresponding author), Med Univ Lubeck, Isotopes Lab, Sect Nat Sci, Ratzeburger Allee 160, D-23538 Lubeck, Germany.	matzanke@isolab.uni-luebeck.de; carrano@sciences.sdsu.edu		Kuepper, Frithjof/0000-0003-1273-7109	joint USA-Germany International Chemistry Collaboration (ICC) grant [NSF CHE-0924313, DFG Ma 916/20-1]; European CommunityEuropean Commission [227788]; Direct For Mathematical & Physical ScienNational Science Foundation (NSF)NSF - Directorate for Mathematical & Physical Sciences (MPS) [0924313] Funding Source: National Science Foundation; Division Of ChemistryNational Science Foundation (NSF)NSF - Directorate for Mathematical & Physical Sciences (MPS) [0924313] Funding Source: National Science Foundation	This work is supported by a joint USA-Germany International Chemistry Collaboration (ICC) grant: NSF CHE-0924313 to CJC and DFG Ma 916/20-1 to BFM. We acknowledge the support of the European Community research infrastructure action under the FP7 'capacities' specific programme ASSEMBLE (grant no. 227788). We thank Professor Kathy Barbeau, SIO/UCSD and her students for determining the iron content of our Scripps Pier water. LHB, CA, and BFM thank DESY, Hamburg for access to their infrastructure (DORIS III beamline at HASYLAB) making possible the EXAFS experiments. LHB thanks Dr E. Welter, HASYLAB, for scientific advice, and EPM and CJC thank Dr Steve Barlow, SDSU Microscopy Center, for help with the histochemistry and microscopy.	Andersen R, 2005, ALGAL CULTURING TECH; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Bauer P, 2003, AGRONOMIE, V23, P447, DOI 10.1051/agro:2003012; BAUMINGER ER, 1980, BIOCHIM BIOPHYS ACTA, V623, P237, DOI 10.1016/0005-2795(80)90252-4; BAUMINGER ER, 1980, J BACTERIOL, V141, P378, DOI 10.1128/JB.141.1.378-381.1980; BOHNKE R, 1995, BIOMETALS, V8, P223; Boughammoura A, 2008, J BACTERIOL, V190, P1518, DOI 10.1128/JB.01640-07; BRULAND KW, 1991, LIMNOL OCEANOGR, V36, P1555, DOI 10.4319/lo.1991.36.8.1555; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chase Z, 2002, J GEOPHYS RES-OCEANS, V107, DOI 10.1029/2001JC000987; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Curie C, 2003, ANNU REV PLANT BIOL, V54, P183, DOI 10.1146/annurev.arplant.54.031902.135018; Dassa E, 2001, RES MICROBIOL, V152, P211, DOI 10.1016/S0923-2508(01)01194-9; FORD GC, 1984, PHILOS T R SOC B, V304, P551, DOI 10.1098/rstb.1984.0046; FROLOW F, 1994, NAT STRUCT BIOL, V1, P453, DOI 10.1038/nsb0794-453; Gutlich P, 2011, MOSSBAUER SPECTROSCOPY AND TRANSITION METAL CHEMISTRY: FUNDAMENTALS AND APPLICATIONS, P201, DOI 10.1007/978-3-540-88428-6_6; Halliwell B., 2007, FREE RADICALS BIOL M; Harrison P. M., 1989, IRON CARRIERS IRON P, P123; HUDSON RJM, 1989, LIMNOL OCEANOGR, V34, P1113, DOI 10.4319/lo.1989.34.6.1113; Johnson KS, 1997, MAR CHEM, V57, P137, DOI 10.1016/S0304-4203(97)00043-1; Koster W, 2001, RES MICROBIOL, V152, P291, DOI 10.1016/S0923-2508(01)01200-1; Kovacs K, 2005, HYPERFINE INTERACT, V165, P289, DOI 10.1007/s10751-006-9288-3; Kovacs K, 2009, PLANTA, V229, P271, DOI 10.1007/s00425-008-0826-x; Kranzler C, 2011, ENVIRON MICROBIOL, V13, P2990, DOI 10.1111/j.1462-2920.2011.02572.x; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kustka AB, 2007, J PHYCOL, V43, P715, DOI 10.1111/j.1529-8817.2007.00359.x; La Fontaine S, 2002, EUKARYOT CELL, V1, P736, DOI 10.1128/EC.1.5.736-757.2002; Lanquar V, 2005, EMBO J, V24, P4041, DOI 10.1038/sj.emboj.7600864; LAWSON DM, 1991, NATURE, V349, P541, DOI 10.1038/349541a0; Liu JW, 2000, J APPL PHYCOL, V12, P605, DOI 10.1023/A:1026523213818; MANLEY SL, 1981, PLANT PHYSIOL, V68, P914, DOI 10.1104/pp.68.4.914; MANN S, 1987, J MOL BIOL, V198, P405, DOI 10.1016/0022-2836(87)90290-7; Marchetti A, 2009, NATURE, V457, P467, DOI 10.1038/nature07539; MARTIN JH, 1988, NATURE, V331, P341, DOI 10.1038/331341a0; Martinoia E, 2007, J EXP BOT, V58, P83, DOI 10.1093/jxb/erl183; MATSUNAGA K, 1991, Bulletin of the Japanese Society of Fisheries Oceanography, V55, P349; Matzanke B F, 1988, Biol Met, V1, P18, DOI 10.1007/BF01128013; Matzanke B. F., 2011, MOSSBAUER SPECTROSCO; Matzanke BF, 2004, BIOCHEMISTRY-US, V43, P1386, DOI 10.1021/bi0357661; Matzanke BF, 1997, TRANSITION METALS IN MICROBIAL METABOLISM, P117; MATZANKE BF, 1987, J BACTERIOL, V169, P5873, DOI 10.1128/jb.169.12.5873-5876.1987; Meguro R, 2007, ARCH HISTOL CYTOL, V70, P1, DOI 10.1679/aohc.70.1; MOOG PR, 1994, PLANT SOIL, V165, P241, DOI 10.1007/BF00008068; Morrissey J, 2009, CHEM REV, V109, P4553, DOI 10.1021/cr900112r; Muller DG, 2008, CAH BIOL MAR, V49, P59; NAITO K, 2001, ANAL SCI, V17, P817; NOLLING J, 1995, EUR J BIOCHEM, V231, P628, DOI 10.1111/j.1432-1033.1995.0628d.x; Paz Y, 2007, PLANT PHYSIOL, V144, P1407, DOI 10.1104/pp.107.100644; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Ravel B, 2005, J SYNCHROTRON RADIAT, V12, P537, DOI 10.1107/S0909049505012719; Ravet K, 2009, PLANT J, V57, P400, DOI 10.1111/j.1365-313X.2008.03698.x; Reindel S, 2002, BIOCHIM BIOPHYS ACTA, V1598, P130; Robinson NJ, 1999, NATURE, V397, P694, DOI 10.1038/17800; ROMHELD V, 1986, PLANT PHYSIOL, V80, P175, DOI 10.1104/pp.80.1.175; Roschzttardtz H, 2010, PLANT SIGNAL BEHAV, V5, P56, DOI 10.4161/psb.5.1.10159; Roschzttardtz H, 2009, PLANT PHYSIOL, V151, P1329, DOI 10.1104/pp.109.144444; Schunemann V, 2000, REP PROG PHYS, V63, P263, DOI 10.1088/0034-4885/63/3/202; Semin BK, 2003, PLANT PHYSIOL, V131, P1756, DOI 10.1104/pp.102.018200; Shaked Y, 2005, LIMNOL OCEANOGR, V50, P872, DOI 10.4319/lo.2005.50.3.0872; Sonier MB, 2010, BIOMETALS, V23, P1029, DOI 10.1007/s10534-010-9348-7; Sutak R, 2010, PLANT PHYSIOL, V154, P991, DOI 10.1104/pp.110.159947; TRICK CG, 1983, SCIENCE, V219, P306, DOI 10.1126/science.219.4582.306; TRIKHA J, 1994, PROTEINS, V18, P107, DOI 10.1002/prot.340180204; WASSERFALLEN A, 1995, J BACTERIOL, V177, P2436, DOI 10.1128/jb.177.9.2436-2441.1995; Weger HG, 2009, BOTANY, V87, P922, DOI 10.1139/B09-063; WINKLER H, 1994, HYPERFINE INTERACT, V91, P841, DOI 10.1007/BF02064616	66	20	20	0	45	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0022-0957	1460-2431		J EXP BOT	J. Exp. Bot.	OCT	2012	63	16					5763	5772		10.1093/jxb/ers225			10	Plant Sciences	Plant Sciences	021XR	WOS:000309919500004	22945940	Bronze, Green Published			2021-04-07	
J	Tarver, JE; Donoghue, PCJ; Peterson, KJ				Tarver, James E.; Donoghue, Philip C. J.; Peterson, Kevin J.			Do miRNAs have a deep evolutionary history?	BIOESSAYS			English	Article						animal; eukaryote; microRNA evolution; plant; protist	SMALL RNAS; MICRORNA GENES; GIARDIA-INTESTINALIS; REGULATORY ROLES; IDENTIFICATION; ARABIDOPSIS; BIOGENESIS; EXPRESSION; ORIGINS; COMPLEXITY	The recent discovery of microRNAs (miRNAs) in unicellular eukaryotes, including miRNAs known previously only from animals or plants, implies that miRNAs have a deep evolutionary history among eukaryotes. This contrasts with the prevailing view that miRNAs evolved convergently in animals and plants. We re-evaluate the evidence and find that none of the 73 plant and animal miRNAs described from protists meet the required criteria for miRNA annotation and, by implication, animals and plants did not acquire any of their respective miRNA genes from the crown ancestor of eukaryotes. Furthermore, of the 159 novel miRNAs previously identified among the seven species of unicellular protists examined, only 28 from the algae Ectocarpus and Chlamydomonas, meet the criteria for miRNA annotation. Therefore, at present only five groups of eukaryotes are known to possess miRNAs, indicating that miRNAs have evolved independently within eukaryotes through exaptation of their shared inherited RNAi machinery.	[Tarver, James E.; Donoghue, Philip C. J.] Univ Bristol, Sch Earth Sci, Bristol, Avon, England; [Tarver, James E.; Peterson, Kevin J.] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA	Tarver, JE (corresponding author), Univ Bristol, Sch Earth Sci, Bristol, Avon, England.	james.tarver@bristol.ac.uk	Donoghue, Philip/A-3873-2008	Donoghue, Philip/0000-0003-3116-7463	EUEuropean Commission; National Aeronautic and Space Agency; National Science FoundationNational Science Foundation (NSF); Natural Environmental Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council; University of Bristol	We would like to thank the editor Andrew Moore and two anonymous reviewers for thoughtful comments on an earlier draft of this manuscript. We acknowledge funding from the Marie Curie actions of EU FP7, the National Aeronautic and Space Agency, the National Science Foundation, the Natural Environmental Research Council, and the University of Bristol.	Ambros V, 2003, RNA, V9, P277, DOI 10.1261/rna.2183803; Arteaga-Vazquez M, 2006, PLANT CELL, V18, P3355, DOI 10.1105/tpc.106.044420; Atayde VD, 2011, TRENDS PARASITOL, V27, P321, DOI 10.1016/j.pt.2011.03.002; Axtell MJ, 2008, TRENDS PLANT SCI, V13, P343, DOI 10.1016/j.tplants.2008.03.009; Axtell MJ, 2011, GENOME BIOL, V12, DOI 10.1186/gb-2011-12-4-221; Bartel DP, 2009, CELL, V136, P215, DOI 10.1016/j.cell.2009.01.002; Bentwich I, 2005, NAT GENET, V37, P766, DOI 10.1038/ng1590; Berezikov E, 2011, NAT REV GENET, V12, P846, DOI 10.1038/nrg3079; Berezikov E, 2011, GENOME RES, V21, P203, DOI 10.1101/gr.116657.110; Berezikov E, 2010, NAT GENET, V42, P6, DOI 10.1038/ng0110-6; Braun L, 2010, PLOS PATHOG, V6, DOI 10.1371/journal.ppat.1000920; Brennecke J, 2005, PLOS BIOL, V3, P404, DOI 10.1371/journal.pbio.0030085; Campbell LI, 2011, P NATL ACAD SCI USA, V108, P15920, DOI 10.1073/pnas.1105499108; Carthew RW, 2009, CELL, V136, P642, DOI 10.1016/j.cell.2009.01.035; Chapman EJ, 2007, NAT REV GENET, V8, P884, DOI 10.1038/nrg2179; Chen XWS, 2011, BMC GENOMICS, V12, DOI 10.1186/1471-2164-12-550; Chen XW, 2009, GENOME BIOL EVOL, V1, P165, DOI 10.1093/gbe/evp017; Chiang HR, 2010, GENE DEV, V24, P992, DOI 10.1101/gad.1884710; Chung WJ, 2008, CURR BIOL, V18, P795, DOI 10.1016/j.cub.2008.05.006; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collins Lesley Joan, 2011, Frontiers in Genetics, V2, P96, DOI 10.3389/fgene.2011.00096; Cuperus JT, 2011, PLANT CELL, V23, P431, DOI 10.1105/tpc.110.082784; de Jong D, 2009, MOL BIOL EVOL, V26, P1333, DOI 10.1093/molbev/msp042; Fahlgren N, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000219; Gough J, 2001, J MOL BIOL, V313, P903, DOI 10.1006/jmbi.2001.5080; Grimson A, 2008, NATURE, V455, P1193, DOI 10.1038/nature07415; Han J, 2009, CELL, V136, P75, DOI 10.1016/j.cell.2008.10.053; Heimberg AM, 2008, P NATL ACAD SCI USA, V105, P2946, DOI 10.1073/pnas.0712259105; Heimberg AM, 2010, P NATL ACAD SCI USA, V107, P19379, DOI 10.1073/pnas.1010350107; Hinas A, 2007, NUCLEIC ACIDS RES, V35, P6714, DOI 10.1093/nar/gkm707; Huang AY, 2011, BMC GENOMICS, V12, DOI 10.1186/1471-2164-12-337; Huang PJ, 2012, GENOMICS, V99, P101, DOI 10.1016/j.ygeno.2011.11.002; Jones-Rhoades MW, 2006, ANNU REV PLANT BIOL, V57, P19, DOI 10.1146/annurev.arplant.57.032905.105218; Kim VN, 2009, NAT REV MOL CELL BIO, V10, P126, DOI 10.1038/nrm2632; Kolev NG, 2011, EUKARYOT CELL, V10, P1156, DOI 10.1128/EC.05114-11; Kolev NG, 2009, TRENDS PARASITOL, V25, P348, DOI 10.1016/j.pt.2009.05.001; Kozomara A, 2011, NUCLEIC ACIDS RES, V39, pD152, DOI 10.1093/nar/gkq1027; Kurihara Y, 2006, RNA, V12, P206, DOI 10.1261/rna.2146906; Lagos-Quintana M, 2001, SCIENCE, V294, P853, DOI 10.1126/science.1064921; Lai EC, 2003, GENOME BIOL, V4, DOI 10.1186/gb-2003-4-7-r42; Lau NC, 2001, SCIENCE, V294, P858, DOI 10.1126/science.1065062; Lee CT, 2007, DNA CELL BIOL, V26, P209, DOI 10.1089/dna.2006.0545; Lee RC, 2001, SCIENCE, V294, P862, DOI 10.1126/science.1065329; LEE RC, 1993, CELL, V75, P843, DOI 10.1016/0092-8674(93)90529-Y; Lee Y, 2002, EMBO J, V21, P4663, DOI 10.1093/emboj/cdf476; Lee YS, 2009, ANNU REV PATHOL-MECH, V4, P199, DOI 10.1146/annurev.pathol.4.110807.092222; Lewis BP, 2003, CELL, V115, P787, DOI 10.1016/S0092-8674(03)01018-3; Li W, 2011, PLOS NEGLECT TROP D, V5, DOI 10.1371/journal.pntd.0001338; Lim LP, 2003, SCIENCE, V299, P1540, DOI 10.1126/science.1080372; Lin WC, 2009, PARASITOL RES, V105, P1683, DOI 10.1007/s00436-009-1616-5; Lin WC, 2009, GENOMICS, V93, P487, DOI 10.1016/j.ygeno.2009.01.004; Liu QA, 2010, PARASITOL RES, V107, P501, DOI 10.1007/s00436-010-1927-6; Llave C, 2002, SCIENCE, V297, P2053, DOI 10.1126/science.1076311; Lu J, 2005, NATURE, V435, P834, DOI 10.1038/nature03702; Lu J, 2008, NAT GENET, V40, P351, DOI 10.1038/ng.73; Lu M, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003420; Lyson TR, 2012, BIOL LETTERS, V8, P104, DOI 10.1098/rsbl.2011.0477; Ma ZR, 2010, PLANT CELL, V22, P1090, DOI 10.1105/tpc.110.073882; MacRae IJ, 2006, SCIENCE, V311, P195, DOI 10.1126/science.1121638; Margis R, 2006, FEBS LETT, V580, P2442, DOI 10.1016/j.febslet.2006.03.072; Meyers BC, 2008, PLANT CELL, V20, P3186, DOI 10.1105/tpc.108.064311; Mi SJ, 2008, CELL, V133, P116, DOI 10.1016/j.cell.2008.02.034; Molnar A, 2007, NATURE, V447, P1126, DOI 10.1038/nature05903; Mourelatos Z, 2002, GENE DEV, V16, P720, DOI 10.1101/gad.974702; Murphy D, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-92; Niwa R, 2007, CURR OPIN GENET DEV, V17, P145, DOI 10.1016/j.gde.2007.02.004; Nozawa M, 2012, GENOME BIOL EVOL, V4, P230, DOI 10.1093/gbe/evs002; Okamura K, 2008, NAT STRUCT MOL BIOL, V15, P354, DOI 10.1038/nsmb.1409; Park MY, 2005, P NATL ACAD SCI USA, V102, P3691, DOI 10.1073/pnas.0405570102; Park W, 2002, CURR BIOL, V12, P1484, DOI 10.1016/S0960-9822(02)01017-5; Peterson KJ, 2007, PALAEONTOLOGY, V50, P775, DOI 10.1111/j.1475-4983.2007.00692.x; Peterson KJ, 2009, BIOESSAYS, V31, P736, DOI 10.1002/bies.200900033; Prucca CG, 2008, NATURE, V456, P750, DOI 10.1038/nature07585; Rajagopalan R, 2006, GENE DEV, V20, P3407, DOI 10.1101/gad.1476406; Reinhart BJ, 2002, GENE DEV, V16, P1616, DOI 10.1101/gad.1004402; Rhoades MW, 2002, CELL, V110, P513, DOI 10.1016/S0092-8674(02)00863-2; Ruby JG, 2007, GENOME RES, V17, P1850, DOI 10.1101/gr.6597907; Saraiya AA, 2011, RNA, V17, P2152, DOI 10.1261/rna.028118.111; Saraiya AA, 2008, PLOS PATHOG, V4, DOI 10.1371/journal.ppat.1000224; Sdassi N, 2009, BMC GENOMICS, V10, DOI 10.1186/1471-2164-10-149; Sempere LF, 2006, J EXP ZOOL PART B, V306B, P575, DOI 10.1002/jez.b.21118; Shabalina SA, 2008, TRENDS ECOL EVOL, V23, P578, DOI 10.1016/j.tree.2008.06.005; Sperling EA, 2010, GEOBIOLOGY, V8, P24, DOI 10.1111/j.1472-4669.2009.00225.x; Starega-Roslan J, 2011, CELL MOL LIFE SCI, V68, P2859, DOI 10.1007/s00018-011-0726-2; Tsutsumi A, 2011, NAT STRUCT MOL BIOL, V18, P1153, DOI 10.1038/nsmb.2125; Wheeler BM, 2009, EVOL DEV, V11, P50, DOI 10.1111/j.1525-142X.2008.00302.x; WIGHTMAN B, 1993, CELL, V75, P855, DOI 10.1016/0092-8674(93)90530-4; Winter J, 2009, NAT CELL BIOL, V11, P228, DOI 10.1038/ncb0309-228; Xie ZX, 2003, CURR BIOL, V13, P784, DOI 10.1016/S0960-9822(03)00281-1; Zhao T, 2007, GENE DEV, V21, P1190, DOI 10.1101/gad.1543507; ZUCKER M, 1999, RNA BIOCH BIOTECHNOL	91	71	72	4	57	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0265-9247	1521-1878		BIOESSAYS	Bioessays	OCT	2012	34	10					857	866		10.1002/bies.201200055			10	Biochemistry & Molecular Biology; Biology	Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics	004WR	WOS:000308712600011	22847169	Bronze			2021-04-07	
J	Heesch, S; Day, JG; Yamagishi, T; Kawai, H; Muller, DG; Kupper, FC				Heesch, Svenja; Day, John G.; Yamagishi, Takahiro; Kawai, Hiroshi; Mueller, Dieter G.; Kuepper, Frithjof C.			CRYOPRESERVATION OF THE MODEL ALGA Ectocarpus (PHAEOPHYCEAE)	CRYOLETTERS			English	Article						Barcoding; brown algae; cryopreservation; Ectocarpus; model organism	FILAMENTOUS BROWN-ALGAE; SILICULOSUS PHAEOPHYCEAE; LIFE-CYCLE; SEX ATTRACTANT; LONG-TERM; GAMETOPHYTES; RHODOPHYTA; CONSERVATION; MAINTENANCE; CONCHOCELIS	The brown alga Ectocarpus has recently become the first fully sequenced multicellular alga and is an important biological model. Due to the large and growing number of Ectocarpus strains isolated and maintained by the research community, including increasing numbers of mutants, there is an urgent need for developing reliable, cost-effective long-term maintenance techniques. We report here that cryopreservation constitutes an attractive option in this respect, using a simple two-step protocol employing combined DMSO 10% (v/v) and sorbitol 9% (w/v) as cryoprotectants. This model organism appears to be remarkably robust and post-cryo recovery has been observed in all strains tested in this study. Cultures can be regenerated by the germination of cryopreserved zooids (spores), or the recovery of vegetative cells. In the latter case, dividing surviving cells may grow into the cell lumen of a neighbouring dead cell, eventually regenerating a phenotypically normal thalloidal structure.	[Heesch, Svenja; Day, John G.; Kuepper, Frithjof C.] Scottish Marine Inst, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland; [Yamagishi, Takahiro; Kawai, Hiroshi] Kobe Univ, Res Ctr Inland Seas, Nada Ku, Kobe, Hyogo 6578501, Japan; [Mueller, Dieter G.] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany	Day, JG (corresponding author), Scottish Marine Inst, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland.	jgd@sams.ac.uk; fck@sams.ac.uk	Day, John/N-5913-2014	Kuepper, Frithjof/0000-0003-1273-7109; Heesch, Svenja/0000-0002-4531-0921	European Commission (ASSEMBLE, Integrated Infrastructures Initiative) [227799]; UK Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NF3 CCAP]; Japanese National Bioresource Project (JST); Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/F012705/1, NE/D521522/1] Funding Source: researchfish	We are grateful to the staff of CCAP for assistance with Ectocarpus culturing and cryopreservation experiments, and to Akira F. Peters for information on Ectocarpus genotypes. This work was supported by the European Commission (ASSEMBLE, Integrated Infrastructures Initiative, grant agreement no. 227799) and the UK Natural Environment Research Council (Oceans 2025 program NF3 CCAP). KU-MACC is partly supported by the Japanese National Bioresource Project (JST).	Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Brodie J, 2009, PHYCOLOGIA, V48, P423, DOI 10.2216/09-21.1; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Coelho SM, 2011, P NATL ACAD SCI USA, V108, P11518, DOI 10.1073/pnas.1102274108; Day JG, 1999, IN VITRO CELL DEV-PL, V35, P127; Day JG, 1997, J APPL PHYCOL, V9, P121, DOI 10.1023/A:1007991507314; Day JG, 2012, BIORESOURCE TECHNOL, V109, P245, DOI 10.1016/j.biortech.2011.05.033; Day JG, 2010, TAXON, V59, P3, DOI 10.1002/tax.591001; Fleck RA, 2000, FREE RADICAL RES, V32, P157, DOI 10.1080/10715760000300161; Fleck RA, 1999, CRYO-LETT, V20, P271; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; GINSBURGERVOGEL T, 1992, AQUACULTURE, V106, P171, DOI 10.1016/0044-8486(92)90201-U; GROUT B, 1990, TRENDS BIOTECHNOL, V8, P293, DOI 10.1016/0167-7799(90)90201-8; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; Kawai H, 2011, JAPAN J PHYCOL, V59, P1; KUHLENKAMP R, 1994, BOT MAR, V37, P525, DOI 10.1515/botm.1994.37.6.525; KUWANO K, 1993, PLANT SCI, V94, P215, DOI 10.1016/0168-9452(93)90022-R; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Lorenz M., 2005, ALGAL CULTURING TECH, P145, DOI 10.1016/b978-012088426-1/50011-1; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; Maier I, 1995, PROGR PHYCOL RES, V11, P51; Milner D. G., 2001, SPRINGER INDEX VIRUS, P732; Muller DG, 2008, CAH BIOL MAR, V49, P59; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; Muller DG, 1997, PHYCOLOGIA, V36, P79, DOI 10.2216/i0031-8884-36-1-79.1; PAPENFUSS GEORGE F., 1933, SCIENCE, V77, P390, DOI 10.1126/science.77.1999.390; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; RATTRAY J, 1885, T ROY SOC EDINBURGH, V32, P589; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Taylor R, 1999, J APPL PHYCOL, V11, P257, DOI 10.1023/A:1008002120416; VANDERMEER JP, 1984, PHYCOLOGIA, V23, P195, DOI 10.2216/i0031-8884-23-2-195.1; Vigneron T, 1997, CRYO-LETT, V18, P93; West JA, 1999, HYDROBIOLOGIA, V399, P101; Zhang QS, 2007, CRYOLETTERS, V28, P215; Zhang QS, 2007, EUR J PHYCOL, V42, P209, DOI 10.1080/09670260701261778; Zhou Wenjun, 2007, Journal of Ocean University of China, V6, P299, DOI 10.1007/s11802-007-0299-8	49	14	14	0	22	CRYO LETTERS	LONDON	C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND	0143-2044			CRYOLETTERS	CryoLetters	SEP-OCT	2012	33	5					327	336					10	Biology; Physiology	Life Sciences & Biomedicine - Other Topics; Physiology	042HW	WOS:000311463300001	23224366				2021-04-07	
J	Dittami, SM; Gravot, A; Goulitquer, S; Rousvoal, S; Peters, AF; Bouchereau, A; Boyen, C; Tonon, T				Dittami, Simon M.; Gravot, Antoine; Goulitquer, Sophie; Rousvoal, Sylvie; Peters, Akira F.; Bouchereau, Alain; Boyen, Catherine; Tonon, Thierry			Towards deciphering dynamic changes and evolutionary mechanisms involved in the adaptation to low salinities in Ectocarpus (brown algae)	PLANT JOURNAL			English	Article						adaptation; brown algae; Ectocarpus; abiotic stress; primary metabolism; transcriptomic and metabolite profiling	LAMINARIA-DIGITATA; SILICULOSUS PHAEOPHYCEAE; ABIOTIC STRESS; REVEALS; MARINE; NORMALIZATION; ARABIDOPSIS; METABOLISM; DIVERSITY; SEQUENCES	Colonizations of freshwater by marine species are rare events, and little information is known about the underlying mechanisms. Brown algae are an independent lineage of photosynthetic and multicellular organisms from which few species inhabit freshwater. As a marine alga that is also found in freshwater, Ectocarpus is of particular interest for studying the transition between these habitats. To gain insights into mechanisms of the transition, we examined salinity tolerance and adaptations to low salinities in a freshwater strain of Ectocarpus on physiological and molecular levels. We show that this isolate belongs to a widely distributed and highly stress-resistant clade, and differed from the genome-sequenced marine strain in its tolerance of low salinities. It also exhibited profound, but reversible, morphological, physiological, and transcriptomic changes when transferred to seawater. Although gene expression profiles were similar in both strains under identical conditions, metabolite and ion profiles differed strongly, the freshwater strain exhibiting e.g. higher cellular contents of amino acids and nitrate, higher contents of n-3 fatty acids, and lower intracellular mannitol and sodium concentrations. Moreover, several stress markers were noted in the freshwater isolate in seawater. This finding suggests that, while high stress tolerance and plasticity may be prerequisites for the colonization of freshwater, genomic alterations have occurred that produced permanent changes in the metabolite profiles to stabilize the transition.	[Dittami, Simon M.; Rousvoal, Sylvie; Boyen, Catherine; Tonon, Thierry] UPMC Univ Paris 6, Biol Stn, UMR Marine Plants & Biomol 7139, F-29680 Roscoff, France; [Dittami, Simon M.; Rousvoal, Sylvie; Boyen, Catherine; Tonon, Thierry] CNRS, Biol Stn, UMR Marine Plants & Biomol 7139, F-29680 Roscoff, France; [Gravot, Antoine; Bouchereau, Alain] Univ Rennes 1, Inst Genet Environm & Protect Plantes, INRA, Agrocampus Ouest,UMR 1349, F-35042 Rennes, France; [Gravot, Antoine; Bouchereau, Alain] Univ Europeenne Bretagne, Rennes, France; [Goulitquer, Sophie] Univ Bretagne Occidentale, Fac Med, Lab Biochim Epissage Canc Lipides & Apoptose, INSERM,U613, F-29285 Brest, France; [Peters, Akira F.] BEZHIN ROSKO, F-29250 Santec, France; [Peters, Akira F.] MBA Lab, Plymouth PL1 2PB, Devon, England	Tonon, T (corresponding author), UPMC Univ Paris 6, Biol Stn, UMR Marine Plants & Biomol 7139, F-29680 Roscoff, France.	tonon@sb-roscoff.fr	Dittami, Simon/E-8354-2011; Lenka, Sangram K./A-5830-2009; Tonon, Thierry/A-3214-2009	Dittami, Simon/0000-0001-7987-7523; Lenka, Sangram K./0000-0002-0121-2430; Tonon, Thierry/0000-0002-1454-6018	European communityEuropean Commission [MESTCT 2005-020737]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [MBA010001] Funding Source: researchfish	We thank Gurvan Michel for providing annotations of genes related to cell wall modifications and for helpful discussions, John Dittami for critical reading of the manuscript and helpful discussion, P.-O. de Franco for providing annotations of genes related to glutathione metabolism, David Renault and Raphael Lugan for help with the MS analysis, Odile Henin for technical assistance with the Dionex analysis, and Laurence Dartevelle for morphological observations of the FWS. SD received funding from the European community's Sixth Framework Programme (contract no. MESTCT 2005-020737).	Akerboom T P, 1981, Methods Enzymol, V77, P373; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Aquino RS, 2005, GLYCOBIOLOGY, V15, P11, DOI 10.1093/glycob/cwh138; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; Berglin M, 2004, BIOMACROMOLECULES, V5, P2376, DOI 10.1021/bm0496864; Bold H. C., 1985, INTRO ALGAE STRUCTUR; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; DAVISON IR, 1985, PHYCOLOGIA, V24, P449, DOI 10.2216/i0031-8884-24-4-449.1; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Dittami SM, 2011, PLANT CELL ENVIRON, V34, P629, DOI 10.1111/j.1365-3040.2010.02268.x; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Drummond AJ, 2007, BMC EVOL BIOL, V7, DOI 10.1186/1471-2148-7-214; FLYNN KJ, 1989, J PLANKTON RES, V11, P165, DOI 10.1093/plankt/11.1.165; GEISSLER U, 1983, NOVA HEDWIGIA, V37, P193; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Irizarry RA, 2003, BIOSTATISTICS, V4, P249, DOI 10.1093/biostatistics/4.2.249; Jubault M, 2008, PLANT PHYSIOL, V146, P2008, DOI 10.1104/pp.108.117432; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lee CE, 1999, TRENDS ECOL EVOL, V14, P284, DOI 10.1016/S0169-5347(99)01596-7; Logares R, 2009, TRENDS MICROBIOL, V17, P414, DOI 10.1016/j.tim.2009.05.010; Lugan R, 2009, PLANT CELL ENVIRON, V32, P95, DOI 10.1111/j.1365-3040.2008.01898.x; McCauley LAR, 2007, PHYCOLOGIA, V46, P429, DOI 10.2216/05-08.1; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Popper ZA, 2011, ANNU REV PLANT BIOL, V62, P567, DOI 10.1146/annurev-arplant-042110-103809; Posada D, 2008, MOL BIOL EVOL, V25, P1253, DOI 10.1093/molbev/msn083; RAVANKO O, 1970, NOVA HEDWIGIA Z KRYP, V20, P179; Raven JA, 1999, PLANT CELL ENVIRON, V22, P741, DOI 10.1046/j.1365-3040.1999.00419.x; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; REED RH, 1983, BOT MAR, V26, P409, DOI 10.1515/botm.1983.26.9.409; Renault H, 2010, BMC PLANT BIOL, V10, DOI 10.1186/1471-2229-10-20; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Roessner U, 2001, PLANT CELL, V13, P11, DOI 10.1105/tpc.13.1.11; Rousvoal S, 2011, PLANTA, V233, P261, DOI 10.1007/s00425-010-1295-6; RUSSELL G, 1975, ESTUAR COAST MAR SCI, V3, P91, DOI 10.1016/0302-3524(75)90008-0; Saeed AI, 2003, BIOTECHNIQUES, V34, P374, DOI 10.2144/03342mt01; Sealfon RSG, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-443; Sigee D., 2005, FRESHWATER MICROBIOL; Silberfeld T, 2010, MOL PHYLOGENET EVOL, V56, P659, DOI 10.1016/j.ympev.2010.04.020; Sorensen I, 2011, PLANT J, V68, P201, DOI 10.1111/j.1365-313X.2011.04686.x; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; West John A., 1996, Muelleria, V9, P29	51	47	47	0	57	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0960-7412	1365-313X		PLANT J	Plant J.	AUG	2012	71	3					366	377		10.1111/j.1365-313X.2012.04982.x			12	Plant Sciences	Plant Sciences	980LA	WOS:000306893500002	22394375	Bronze			2021-04-07	
J	Hamacher, K; Greiner, T; Ogata, H; Van Etten, JL; Gebhardt, M; Villarreal, LP; Cosentino, C; Moroni, A; Thiel, G				Hamacher, Kay; Greiner, Timo; Ogata, Hiroyuki; Van Etten, James L.; Gebhardt, Manuela; Villarreal, Luis P.; Cosentino, Cristian; Moroni, Anna; Thiel, Gerhard			Phycodnavirus Potassium Ion Channel Proteins Question the Virus Molecular Piracy Hypothesis	PLOS ONE			English	Article							ECTOCARPUS-SILICULOSUS VIRUS; MULTIPLE SEQUENCE ALIGNMENT; VIRAL K+ CHANNELS; CHLORELLA VIRUSES; HOST-CELLS; KCV; INFECTION; GENOME; NC64A; EUKARYOTES	Phycodnaviruses are large dsDNA, algal-infecting viruses that encode many genes with homologs in prokaryotes and eukaryotes. Among the viral gene products are the smallest proteins known to form functional K+ channels. To determine if these viral K+ channels are the product of molecular piracy from their hosts, we compared the sequences of the K+ channel pore modules from seven phycodnaviruses to the K+ channels from Chlorella variabilis and Ectocarpus siliculosus, whose genomes have recently been sequenced. C. variabilis is the host for two of the viruses PBCV-1 and NY-2A and E. siliculosus is the host for the virus EsV-1. Systematic phylogenetic analyses consistently indicate that the viral K+ channels are not related to any lineage of the host channel homologs and that they are more closely related to each other than to their host homologs. A consensus sequence of the viral channels resembles a protein of unknown function from a proteobacterium. However, the bacterial protein lacks the consensus motif of all K+ channels and it does not form a functional channel in yeast, suggesting that the viral channels did not come from a proteobacterium. Collectively, our results indicate that the viruses did not acquire their K+ channel-encoding genes from their current algal hosts by gene transfer; thus alternative explanations are required. One possibility is that the viral genes arose from ancient organisms, which served as their hosts before the viruses developed their current host specificity. Alternatively the viral proteins could be the origin of K+ channels in algae and perhaps even all cellular organisms.	[Hamacher, Kay] Tech Univ Darmstadt, Computat Biol Grp, Darmstadt, Germany; [Greiner, Timo; Gebhardt, Manuela; Thiel, Gerhard] Tech Univ Darmstadt, Membrane Biophys Grp, Darmstadt, Germany; [Ogata, Hiroyuki] Aix Marseille Univ, Struct & Genom Informat Lab, Marseille, France; [Van Etten, James L.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA; [Van Etten, James L.] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE USA; [Villarreal, Luis P.] Univ Calif Irvine, Ctr Virus Res, Irvine, CA USA; [Cosentino, Cristian; Moroni, Anna] Univ Milan, Dept Biol, Milan, Italy	Hamacher, K (corresponding author), Tech Univ Darmstadt, Computat Biol Grp, Darmstadt, Germany.	thiel@bio.tu-darmstadt.de	Cosentino, Cristian/F-2604-2010; moroni, anna/H-2097-2014; Greiner, Timo/Y-1900-2019	Cosentino, Cristian/0000-0002-2784-0048; moroni, anna/0000-0002-1860-406X; Greiner, Timo/0000-0002-0909-570X; Thiel, Gerhard/0000-0002-2335-1351	Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [DFG GrK-1657]; Public Health Service GrantUnited States Public Health Service [GM32441]; Fonds der Chemischen IndustrieFonds der Chemischen IndustrieEuropean Commission; National Institutes of Health grant from the COBRE program of the National Center for Research ResourcesUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [P20RR15635]; European Drug Initiative on Channels and Transporters (EDICT) project EU FP7 [201924]; NATIONAL CENTER FOR RESEARCH RESOURCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635, P20RR015635] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441, R01GM032441] Funding Source: NIH RePORTER	Study was supported by: 1) Deutsche Forschungsgemeinschaft (DFG GrK-1657: GT, and KH), 2) Public Health Service Grant GM32441 (JLVE), 3) Fonds der Chemischen Industrie (KH), 4) National Institutes of Health grant P20RR15635 from the COBRE program of the National Center for Research Resources (JLVE) 5) European Drug Initiative on Channels and Transporters (EDICT) project EU FP7 (201924) (AM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Balss J, 2008, P NATL ACAD SCI USA, V105, P12313, DOI 10.1073/pnas.0805709105; Blanc G, 2010, PLANT CELL, V22, P2943, DOI 10.1105/tpc.110.076406; Chen J, 2005, BIOCHEM BIOPH RES CO, V326, P887, DOI 10.1016/j.bbrc.2004.11.125; Ciampor F., 2003, Acta Microbiologica et Immunologica Hungarica, V50, P433, DOI 10.1556/AMicr.50.2003.4.9; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Dereeper A, 2008, NUCLEIC ACIDS RES, V36, pW465, DOI 10.1093/nar/gkn180; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Felsenstein J., 1989, CLADISTICS, V5, P164, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X; Fischer WB, 2002, BBA-BIOMEMBRANES, V1561, P27, DOI 10.1016/S0304-4157(01)00009-0; Fitzgerald LA, 2007, VIROLOGY, V362, P350, DOI 10.1016/j.virol.2006.12.028; Fitzgerald LA, 2007, VIROLOGY, V358, P459, DOI 10.1016/j.virol.2006.08.034; Fitzgerald LA, 2007, VIROLOGY, V358, P472, DOI 10.1016/j.virol.2006.08.033; Frohns F, 2006, J VIROL, V80, P2437, DOI 10.1128/JVI.80.5.2437-2444.2006; Gazzarrini S, 2006, P NATL ACAD SCI USA, V103, P5355, DOI 10.1073/pnas.0600848103; Gazzarrini S, 2004, J BIOL CHEM, V279, P28443, DOI 10.1074/jbc.M401184200; Gazzarrini S, 2003, FEBS LETT, V552, P12, DOI 10.1016/S0014-5793(03)00777-4; Gazzarrini S, 2009, BIOCHEM J, V420, P295, DOI 10.1042/BJ20090095; Gonzalez ME, 2003, FEBS LETT, V552, P28, DOI 10.1016/S0014-5793(03)00780-4; Greiner T, 2011, PLANT J, V68, P977, DOI 10.1111/j.1365-313X.2011.04748.x; Greiner T, 2009, J GEN VIROL, V90, P2033, DOI 10.1099/vir.0.010629-0; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; HEGINBOTHAM L, 1994, BIOPHYS J, V66, P1061, DOI 10.1016/S0006-3495(94)80887-2; Hsu K, 2004, MOL CELL, V14, P259, DOI 10.1016/S1097-2765(04)00183-2; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; Kang M, 2004, P NATL ACAD SCI USA, V101, P5318, DOI 10.1073/pnas.0307824100; Kwan T, 2005, P NATL ACAD SCI USA, V102, P5174, DOI 10.1073/pnas.0501140102; Lesage F, 1996, EMBO J, V15, P1004, DOI 10.1002/j.1460-2075.1996.tb00437.x; Moreira D, 2009, NAT REV MICROBIOL, V7, P306, DOI 10.1038/nrmicro2108; Neupartl M, 2008, VIROLOGY, V372, P340, DOI 10.1016/j.virol.2007.10.024; Ogata H, 2007, GENOME RES, V17, P1353, DOI 10.1101/gr.6358607; Ogata H, 2011, ISME J, V5, P1143, DOI 10.1038/ismej.2010.210; Pagliuca C, 2007, BIOCHEMISTRY-US, V46, P1079, DOI 10.1021/bi061530w; Plugge B, 2000, SCIENCE, V287, P1641, DOI 10.1126/science.287.5458.1641; Ronquist F, 2003, BIOINFORMATICS, V19, P1572, DOI 10.1093/bioinformatics/btg180; Shim JW, 2007, FEBS LETT, V581, P1027, DOI 10.1016/j.febslet.2007.02.005; Tayefeh S, 2009, BIOPHYS J, V96, P485, DOI 10.1016/j.bpj.2008.09.050; Thiel G, 2011, BBA-BIOMEMBRANES, V1808, P580, DOI 10.1016/j.bbamem.2010.04.008; Thiel G, 2010, PROG BOT, V71, P169, DOI 10.1007/978-3-642-02167-1_7; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Van Etten JL, TRENDS PL SCI, V17, P1; Villarreal LP, 2008, ORIGIN AND EVOLUTION OF VIRUSES, 2ND EDITION, P477, DOI 10.1016/B978-0-12-374153-0.00021-7; Wang K., 2010, BIOCHIM BIOPHYS ACTA, V29, P2059; Wilson WH, 2009, CURR TOP MICROBIOL, V328, P1; Yin YB, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-24; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Yoshida T, 2011, VIROL J, V8, DOI 10.1186/1743-422X-8-427	50	13	14	0	12	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	JUN 7	2012	7	6							e38826	10.1371/journal.pone.0038826			9	Multidisciplinary Sciences	Science & Technology - Other Topics	959XO	WOS:000305351700074	22685610	DOAJ Gold, Green Published			2021-04-07	
J	Zambounis, A; Elias, M; Sterck, L; Maumus, F; Gachon, CMM				Zambounis, Antonios; Elias, Marek; Sterck, Lieven; Maumus, Florian; Gachon, Claire M. M.			Highly Dynamic Exon Shuffling in Candidate Pathogen Receptors ... What if Brown Algae Were Capable of Adaptive Immunity?	MOLECULAR BIOLOGY AND EVOLUTION			English	Article						brown alga; Ectocarpus; exon shuffling; resistance gene analogue; innate immunity; adaptive immunity	PLANT; EVOLUTION; REPEAT; RECOGNITION; DOMAIN; ALGORITHM; SEQUENCE; GENOME; FAMILY; GENES	Pathogen recognition is the first step of immune reactions. In animals and plants, direct or indirect pathogen recognition is often mediated by a wealth of fast-evolving receptors, many of which contain ligand-binding and signal transduction domains, such as leucine-rich or tetratricopeptide repeat (LRR/TPR) and NB-ARC domains, respectively. In order to identify candidates potentially involved in algal defense, we mined the genome of the brown alga Ectocarpus siliculosus for homologues of these genes and assessed the evolutionary pressures acting upon them. We thus annotated all Ectocarpus LRR-containing genes, in particular an original group of LRR-containing GTPases of the ROCO family, and 24 NB-ARC-TPR proteins. They exhibit high birth and death rates, while a diversifying selection is acting on their LRR (respectively TPR) domain, probably affecting the ligand-binding specificities. Remarkably, each repeat is encoded by an exon, and the intense exon shuffling underpins the variability of LRR and TPR domains. We conclude that the Ectocarpus ROCO and NB-ARC-TPR families are excellent candidates for being involved in recognition/transduction events linked to immunity. We further hypothesize that brown algae may generate their immune repertoire via controlled somatic recombination, so far only known from the vertebrate adaptive immune systems.	[Gachon, Claire M. M.] Scottish Associat Marine Sci, Microbial & Mol Biol Dept, Scottish Marine Inst, Oban, Argyll, Scotland; [Zambounis, Antonios] Univ Thessaly, Sch Agr Sci, Dept Ichthyol & Aquat Environm, Volos, Greece; [Elias, Marek] Univ Ostrava, Fac Sci, Dept Biol & Ecol, CZ-70103 Ostrava, Czech Republic; [Sterck, Lieven] VIB, Dept Plant Syst Biol, Ghent, Belgium; [Sterck, Lieven] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9000 Ghent, Belgium; [Maumus, Florian] INRA Ctr Versailles Grignon, Unite Rech Genom Info, UR 1164, Versailles, France	Gachon, CMM (corresponding author), Scottish Associat Marine Sci, Microbial & Mol Biol Dept, Scottish Marine Inst, Oban, Argyll, Scotland.	cmmg@sams.ac.uk	Maumus, Florian/O-5426-2016; Sterck, Lieven/A-9439-2016; Elias, Marek/D-6851-2014	Maumus, Florian/0000-0001-7325-0527; Sterck, Lieven/0000-0001-7116-4000; Elias, Marek/0000-0003-0066-6542; Gachon, Claire/0000-0002-3702-7472	Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/F012705/1, NE/J00460X/1]; FP7 Marie Curie award [PERG03-GA-2008-230865]; Czech Science FoundationGrant Agency of the Czech Republic [P305/10/0205]; ASSEMBLE [227799]; Scottish Association for Marine Science; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [dml010007] Funding Source: researchfish	Anne-Flore Bonvalot (Ecole Polytechnique, France), J. Mark Cock (Centre National pour la Recherche Scientifique, Roscoff, France), Valerie Geffroy (Universite Paris-Sud, France), Pierre Rouze, Yves van de Peer (Plant Systems Biology, Ghent, Belgium), and three anonymous reviewers are gratefully acknowledged for stimulating discussions and constructive suggestions. This work was supported by the Natural Environment Research Council (Strategic Ocean Funding Initiative NE/F012705/1 and New Investigator grant NE/J00460X/1 to C.M.M.G.); an FP7 Marie Curie award (PERG03-GA-2008-230865 to C.M.M.G.); the Czech Science Foundation (grant number P305/10/0205 to M.E.); the FP7 "Capacities'' Specific Programme ASSEMBLE (grant number 227799 to A.Z.), and a research bursary from the Scottish Association for Marine Science to A.Z.	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Blatch GL, 1999, BIOESSAYS, V21, P932, DOI 10.1002/(SICI)1521-1878(199911)21:11<932::AID-BIES5>3.3.CO;2-E; Boller T, 2009, ANNU REV PLANT BIOL, V60, P379, DOI 10.1146/annurev.arplant.57.032905.105346; Bosgraaf L, 2003, BBA-MOL CELL RES, V1643, P5, DOI 10.1016/j.bbamcr.2003.08.008; Casasoli M, 2009, P NATL ACAD SCI USA, V106, P7666, DOI 10.1073/pnas.0812625106; Castresana J, 2000, MOL BIOL EVOL, V17, P540, DOI 10.1093/oxfordjournals.molbev.a026334; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Daniels V, 2011, NEUROSIGNALS, V19, P1, DOI 10.1159/000324488; Di Matteo A, 2003, P NATL ACAD SCI USA, V100, P10124, DOI 10.1073/pnas.1733690100; Edgar RC, 2004, BMC BIOINFORMATICS, V5, P1, DOI 10.1186/1471-2105-5-113; Ellis J, 2000, CURR OPIN PLANT BIOL, V3, P278, DOI 10.1016/S1369-5266(00)00080-7; Fares MA, 2004, BIOINFORMATICS, V20, P2867, DOI 10.1093/bioinformatics/bth303; Felsenstein J, 2005, PHYLIP PHYLOGENY INF; Flajnik MF, 2010, NAT REV GENET, V11, P47, DOI 10.1038/nrg2703; Friedman AR, 2007, CURR OPIN GENET DEV, V17, P493, DOI 10.1016/j.gde.2007.08.014; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Gotthardt K, 2008, EMBO J, V27, P2239, DOI 10.1038/emboj.2008.150; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Han BW, 2008, SCIENCE, V321, P1834, DOI 10.1126/science.1162484; Hancks DC, 2009, GENOME RES, V19, P1983, DOI 10.1101/gr.093153.109; Keeling PJ, 2005, TRENDS ECOL EVOL, V20, P670, DOI 10.1016/j.tree.2005.09.005; Kiefer F, 2009, NUCLEIC ACIDS RES, V37, pD387, DOI 10.1093/nar/gkn750; Kumar H, 2009, BIOCHEM J, V420, P1, DOI 10.1042/BJ20090272; LI WH, 1993, J MOL EVOL, V36, P96, DOI 10.1007/BF02407308; Lynch M, 2000, SCIENCE, V290, P1151, DOI 10.1126/science.290.5494.1151; Lynn DJ, 2004, IMMUNOGENETICS, V56, P170, DOI 10.1007/s00251-004-0675-0; Lynn DJ, 2004, MOL BIOL EVOL, V21, P819, DOI 10.1093/molbev/msh084; Maekawa T, 2011, NAT IMMUNOL, V12, P818, DOI 10.1038/ni.2083; Marin I, 2008, FASEB J, V22, P3103, DOI 10.1096/fj.08-111310; Morgante M, 2005, NAT GENET, V37, P997, DOI 10.1038/ng1615; Nei M, 2005, ANNU REV GENET, V39, P121, DOI 10.1146/annurev.genet.39.073003.112240; Nicholas KB, 1997, GENEDOC TOOL EDITING; Padmanabhan M, 2009, CELL MICROBIOL, V11, P191, DOI 10.1111/j.1462-5822.2008.01260.x; Pancer Z, 2004, NATURE, V430, P174, DOI 10.1038/nature02740; Patthy L, 1999, GENE, V238, P103, DOI 10.1016/S0378-1119(99)00228-0; Pei JM, 2008, NUCLEIC ACIDS RES, V36, P2295, DOI 10.1093/nar/gkn072; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Povelones M, 2009, SCIENCE, V324, P258, DOI 10.1126/science.1171400; Ronald PC, 2010, SCIENCE, V330, P1061, DOI 10.1126/science.1189468; Stamatakis A, 2008, SYST BIOL, V57, P758, DOI 10.1080/10635150802429642; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Thompson JD, 1997, NUCLEIC ACIDS RES, V25, P4876, DOI 10.1093/nar/25.24.4876; Ting JPY, 2005, CLIN IMMUNOL, V115, P33, DOI 10.1016/j.clim.2005.02.007; van der Biezen EA, 1998, CURR BIOL, V8, pR226, DOI 10.1016/S0960-9822(98)70145-9; Yang ZH, 2000, MOL BIOL EVOL, V17, P32, DOI 10.1093/oxfordjournals.molbev.a026236; Yang ZH, 2007, MOL BIOL EVOL, V24, P1586, DOI 10.1093/molbev/msm088	46	21	24	0	16	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0737-4038	1537-1719		MOL BIOL EVOL	Mol. Biol. Evol.	APR	2012	29	4					1263	1276		10.1093/molbev/msr296			14	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	915IQ	WOS:000302018100015	22144640	Green Published, Bronze			2021-04-07	
J	Zambounis, A; Gaquerel, E; Strittmatter, M; Salaun, JP; Potin, P; Kupper, FC				Zambounis, Antonios; Gaquerel, Emmanuel; Strittmatter, Martina; Salauen, Jean-Pierre; Potin, Philippe; Kuepper, Frithjof C.			Prostaglandin A(2) triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?	ALGAE			English	Article								We report an oxidative burst triggered by prostaglandin A(2) (PGA(2)) in the brown algal kelp Laminaria digitata, constituting the first such discovery in an alga and the second finding of an oxidative burst triggered by a prostaglandin in a living organism. The response is more powerful than the oxidative burst triggered by most other chemical elicitors in Laminaria. Also, it is dose-dependent and cannot be inhibited by diphenylene iodonium, suggesting that another source than NAD(P)H oxidase is operational in the production of reactive oxygen species. Despite the very strong oxidative response, rather few effects at other levels of signal transduction pathways could be identified. PGA, does not increase lipolysis (free fatty acids) in Laminaria, and only one oxylipin (15-hydroxyeicosatetraenoic acid; 15-HETE) was found to be upregulated in Laminaria. In a subsequent set of experiments in the genome model Ectocarpus siliculosus, none of 5 selected candidate genes, all established participants in various stress responses, showed any significant differences in their expression profiles.		Kupper, FC (corresponding author), Univ Paris 06, CNRS, UMR 7139, Biol Stn, BP 74, F-29682 Roscoff, Brittany, France.	fkuepper@abdn.ac.uk	Gaquerel, Emmanuel/I-2503-2014; Gaquerel, Emmanuel/ABE-6313-2020	Gaquerel, Emmanuel/0000-0003-0796-6417; Gaquerel, Emmanuel/0000-0003-0796-6417; Strittmatter, Martina/0000-0002-1258-9751; Kuepper, Frithjof/0000-0003-1273-7109	Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [dml010007] Funding Source: researchfish		Andreou A, 2009, PROG LIPID RES, V48, P148, DOI 10.1016/j.plipres.2009.02.002; Arimura G, 2000, NATURE, V406, P512, DOI 10.1038/35020072; Arnold TM, 2001, J PHYCOL, V37, P1026, DOI 10.1046/j.1529-8817.2001.01130.x; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Baldridge CW, 1933, AM J PHYSIOL, V103, P235; Bestwick C, 1999, TRENDS PLANT SCI, V4, P88, DOI 10.1016/S1360-1385(99)01385-0; Bolwell GP, 1998, PLANT PHYSIOL, V116, P1379, DOI 10.1104/pp.116.4.1379; Bouarab K, 2004, PLANT PHYSIOL, V135, P1838, DOI 10.1104/pp.103.037622; Bouarab K, 1999, PLANT CELL, V11, P1635, DOI 10.1105/tpc.11.9.1635; Carpenter LJ, 2000, GLOBAL BIOGEOCHEM CY, V14, P1191, DOI 10.1029/2000GB001257; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; COLLEN J, 1994, PHYSIOL PLANTARUM, V92, P417; Colombo ML, 2006, PLANT FOOD HUM NUTR, V61, P67, DOI 10.1007/s11130-006-0015-7; Cosse A., 2007, ADV BOT RES, V46, P221, DOI DOI 10.1016/S0065-2296(07)46006-2; Dang HT, 2010, ARCH PHARM RES, V33, P1325, DOI 10.1007/s12272-010-0905-y; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dring MJ, 2006, ADV BOT RES, V43, P175, DOI 10.1016/S0065-2296(05)43004-9; Ellertsdottir E, 1997, MAR ECOL PROG SER, V146, P135, DOI 10.3354/meps146135; Farmer E, 2005, CURR OPIN PLANT BIOL, V8, P343, DOI 10.1016/j.pbi.2005.05.011; Farmer EE, 2003, CURR OPIN PLANT BIOL, V6, P372, DOI 10.1016/S1369-5266(03)00045-1; Funk CD, 2001, SCIENCE, V294, P1871, DOI 10.1126/science.294.5548.1871; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gaquerel E, 2007, BBA-MOL CELL BIOL L, V1771, P565, DOI 10.1016/j.bbalip.2007.02.007; GERHART DJ, 1984, MAR ECOL PROG SER, V19, P181, DOI 10.3354/meps019181; GERWICK WH, 1994, BBA-LIPID LIPID MET, V1211, P243, DOI 10.1016/0005-2760(94)90147-3; GLAZENER JA, 1991, PHYSIOL MOL PLANT P, V39, P123, DOI 10.1016/0885-5765(91)90023-B; Gleason FH, 2011, MAR FRESHWATER RES, V62, P383, DOI 10.1071/MF10294; Higa T, 2000, J TOXICOL-TOXIN REV, V19, P119, DOI 10.1081/TXR-100100317; Hofmann M, 1997, PLANT CELL PHYSIOL, V38, P1046, DOI 10.1093/oxfordjournals.pcp.a029270; Hsu BY, 2008, J FOOD DRUG ANAL, V16, P59; Huang WC, 2002, EXP CELL RES, V277, P192, DOI 10.1006/excr.2002.5546; Imbs AB, 2001, PHYTOCHEMISTRY, V58, P1067, DOI 10.1016/S0031-9422(01)00321-1; Jaworek J, 2001, J PHYSIOL PHARMACOL, V52, P107; KLIEWER SA, 1995, CELL, V83, P813, DOI 10.1016/0092-8674(95)90194-9; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Kupper FC, 2009, PLANT CELL PHYSIOL, V50, P789, DOI 10.1093/pcp/pcp023; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; MILLER CC, 1990, J NUTR, V120, P36; Milner D. G., 2001, SPRINGER INDEX VIRUS, P732; Mittler R, 2004, TRENDS PLANT SCI, V9, P490, DOI 10.1016/j.tplants.2004.08.009; Muller DG, 2008, CAH BIOL MAR, V49, P59; ODONNELL VB, 1993, BIOCHEM J, V290, P41, DOI 10.1042/bj2900041; Orozco-Cardenas ML, 2001, PLANT CELL, V13, P179, DOI 10.1105/tpc.13.1.179; OTTONELLO L, 1995, CLIN EXP IMMUNOL, V101, P502; Pan ZQ, 1998, J BIOL CHEM, V273, P18139, DOI 10.1074/jbc.273.29.18139; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Pfaffl MW, 2002, NUCLEIC ACIDS RES, V30, DOI 10.1093/nar/30.9.e36; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Reymond P, 1998, CURR OPIN PLANT BIOL, V1, P404, DOI 10.1016/S1369-5266(98)80264-1; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Ross C, 2006, CHEM BIOL, V13, P353, DOI 10.1016/j.chembiol.2006.01.009; Ross C, 2005, J PHYCOL, V41, P531, DOI 10.1111/j.1529-8817.2005.04072.x; Sajiki J, 1997, FISHERIES SCI, V63, P128, DOI 10.2331/fishsci.63.128; Schultz JC, 2004, ECOLOGY, V85, P70, DOI 10.1890/02-0704; Spolaore P, 2006, J BIOSCI BIOENG, V101, P87, DOI 10.1263/jbb.101.87; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Strittmatter M, 2009, OOMYCETE GENETICS GE, P25; Turlings TCJ, 2000, J CHEM ECOL, V26, P189, DOI 10.1023/A:1005449730052; Vaidya S, 1999, J IMMUNOL, V163, P6187; Weinberger F, 1999, J PHYCOL, V35, P747, DOI 10.1046/j.1529-8817.1999.3540747.x; Weinberger F, 2000, J APPL PHYCOL, V12, P139, DOI 10.1023/A:1008119125911; Weinberger F, 2000, J PHYCOL, V36, P1079, DOI 10.1046/j.1529-8817.2000.00003.x; West JA, 1999, HYDROBIOLOGIA, V399, P101; Whalen KE, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0008537; Wiesemeier T, 2008, J CHEM ECOL, V34, P1523, DOI 10.1007/s10886-008-9568-2; Wojtaszek P, 1997, BIOCHEM J, V322, P681, DOI 10.1042/bj3220681; Wright E. P, 1877, T R IRISH ACAD, V26, P369	73	7	7	0	11	KOREAN SOC PHYCOLOGY	SEOUL	B1F, TRUST TOWER, 275-7 YANGJAE-DONG, SEOCHO-KU, SEOUL, 137-739, SOUTH KOREA	1226-2617	2093-0860		ALGAE-SEOUL	Algae	MAR	2012	27	1					21	32		10.4490/algae.2012.27.1.021			12	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	914RB	WOS:000301968200003		Green Published			2021-04-07	
J	Brown, MT; Newman, JE; Han, T				Brown, Murray T.; Newman, James E.; Han, Taejun			Inter-population comparisons of copper resistance and accumulation in the red seaweed, Gracilariopsis longissima	ECOTOXICOLOGY			English	Article						Copper; Resistance; Accumulation; Depuration; Inter-population variation; (Active) Biomonitoring; Gracilariopsis longissima; Rhodophyceae	ALGA LOBOPHORA-VARIEGATA; MARINE FOULING ALGA; HEAVY-METALS; ASCOPHYLLUM-NODOSUM; PHYSIOLOGICAL-RESPONSES; ECTOCARPUS-SILICULOSUS; TOLERANCE; MACROALGAE; TOXICITY; BIOINDICATOR	Copper (Cu) resistance and accumulation of five populations of the red seaweed Gracilariopsis longissima collected from sites in south west England (Fal Estuary, Helford Estuary and Chesil Fleet) that differ in their degree of Cu contamination was assessed under controlled laboratory conditions, on two separate occasions (April and October). The effects of a range of Cu concentrations (0-250 mu g l(-1)) on relative growth rates was the same for all populations with reductions observable at concentrations as low as 12 mu g l(-1) and cessation of growth at 250 mu g l(-1). There was no significant difference in the calculated EC50 values for the April and October samples, with means of 31.1 and 25.8 mu g l(-1), respectively. Over the range of concentrations used in this study, copper content increased linearly and the pattern of accumulation was the same for all populations at both time periods. From the linear regressions of the pooled data a concentration factor of 2.25 x 10(3) was calculated. These results imply that G. longissima has an innate tolerance to Cu and that populations have not evolved copper-tolerant ecotypes. In laboratory studies, accumulated Cu was released when transferred to 'clean' seawater with approximately 80% being lost after 8 days, with no significant difference between populations in their response. The results from a 30 days in situ transplantation experiment using two populations from the Fal Estuary provided further evidence for dynamic changes in Cu content in response to changes in Cu bioavailability. The findings in this study are discussed in the context of implications for seaweed biomonitoring.	[Brown, Murray T.; Newman, James E.] Univ Plymouth, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England; [Han, Taejun] Univ Incheon, Div Biol & Chem, Inchon 402749, South Korea	Brown, MT (corresponding author), Univ Plymouth, Sch Marine Sci & Engn, Plymouth PL4 8AA, Devon, England.	mtbrown@plymouth.ac.uk	Brown, Murray/K-5291-2014	Brown, Murray/0000-0003-2655-8611	Astra Zeneca (Brixham Environment Laboratory)	We gratefully acknowledge financial support to JEN from Astra Zeneca (Brixham Environment Laboratory).	Amado GM, 1999, MAR ENVIRON RES, V48, P213, DOI 10.1016/S0141-1136(99)00042-2; ANDERSON BS, 1990, MAR ECOL PROG SER, V68, P147, DOI 10.3354/meps068147; Andrade S, 2010, CHEMOSPHERE, V78, P397, DOI 10.1016/j.chemosphere.2009.11.006; Bennett A.F., 1987, P147; Boubonari T, 2008, BOT MAR, V51, P472, DOI 10.1515/BOT.2008.059; Brown M.T., 1998, METABOLISM TRACE MET, P185; Brown MT, 2003, AQUAT TOXICOL, V64, P201, DOI 10.1016/S0166-445X(03)00054-7; Brown MT, 1998, MAR ENVIRON RES, V47, P1; BRYAN GW, 1992, ENVIRON POLLUT, V76, P89, DOI 10.1016/0269-7491(92)90099-V; BRYAN GW, 1973, J MAR BIOL ASSOC UK, V53, P705, DOI 10.1017/S0025315400058902; Bryan GW, 1983, MAR BIOL ASS OCCAS P; CHAPHEKAR SB, 1991, J ENVIRON BIOL, V12, P163; Contreras L, 2005, J PHYCOL, V41, P1184, DOI 10.1111/j.1529-8817.2005.00151.x; Correa JA, 1996, ENVIRON MONIT ASSESS, V40, P41, DOI 10.1007/BF00395166; CRITCHLEY AT, 1993, SEAWEED CULTIVATION, P89; Deng H, 2006, ENVIRON POLLUT, V141, P69, DOI 10.1016/j.envpol.2005.08.015; DOUST JL, 1994, BIOL REV, V69, P147, DOI 10.1111/j.1469-185X.1994.tb01504.x; EDWARDS P, 1972, Marine Pollution Bulletin, V3, P184, DOI 10.1016/0025-326X(72)90266-4; EIDE I, 1980, ENVIRON POLLUT A, V23, P19, DOI 10.1016/0143-1471(80)90093-8; ERNST WHO, 1992, ACTA BOT NEERL, V41, P229, DOI 10.1111/j.1438-8677.1992.tb01332.x; Garcia-Rios V, 2007, AQUAT TOXICOL, V81, P65, DOI 10.1016/j.aquatox.2006.11.001; Gledhill M, 1997, J PHYCOL, V33, P2, DOI 10.1111/j.0022-3646.1997.00002.x; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; Han T, 2008, AQUAT TOXICOL, V86, P176, DOI 10.1016/j.aquatox.2007.10.016; Hedouin L, 2008, MAR ENVIRON RES, V66, P438, DOI 10.1016/j.marenvres.2008.07.005; HO YB, 1984, CONSERV RECYCLING, V7, P329, DOI 10.1016/0361-3658(84)90031-6; KLERKS PL, 1987, ENVIRON POLLUT, V45, P173, DOI 10.1016/0269-7491(87)90057-1; Langston W.J., 1995, METAL SPECIATION BIO, P407; Langston WJ, 2003, MAR BIOL ASS OCCAS P, P160; Langston WJ, 2003, MAR BIOL ASS OCCAS P, P154; Lobban C.S., 1994, SEAWEED ECOLOGY PHYS, P366; Macnair MR, 2000, PHYTOREMEDIATION OF CONTAMINATED SOIL AND WATER, P235; MALEA P, 1994, BOT MAR, V37, P505, DOI 10.1515/botm.1994.37.6.505; Maskarola K, 2008, ENVIRON POLLUT, V156, P897; MCNAUGHT.SJ, 1974, ECOLOGY, V55, P1163, DOI 10.2307/1940369; Metian M, 2008, J EXP MAR BIOL ECOL, V362, P49, DOI 10.1016/j.jembe.2008.05.013; MYKLESTAD S, 1978, ENVIRON POLLUT, V16, P277, DOI 10.1016/0013-9327(78)90078-2; Nielsen HD, 2003, NEW PHYTOL, V160, P157, DOI 10.1046/j.1469-8137.2003.00864.x; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; Phillips D. J. H., 1994, BIOMONITORING COASTA, P85; PHILLIPS DJH, 1977, ENVIRON POLLUT, V13, P281, DOI 10.1016/0013-9327(77)90047-7; Pinto E, 2003, J PHYCOL, V39, P1008, DOI 10.1111/j.0022-3646.2003.02-193.x; Rainbow PS, 1995, MAR POLLUT BULL, V31, P183, DOI 10.1016/0025-326X(95)00116-5; REED RH, 1983, J EXP MAR BIOL ECOL, V69, P85, DOI 10.1016/0022-0981(83)90173-9; Rohlf F.J., 1995, BIOMETRY PRINCIPLES, P887; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; SANTELICES B, 1995, J APPL PHYCOL, V7, P501, DOI 10.1007/BF00003935; Sawidis T, 2001, ENVIRON INT, V27, P43, DOI 10.1016/S0160-4120(01)00052-6; SEELIGER U, 1979, J MAR BIOL ASSOC UK, V59, P227, DOI 10.1017/S0025315400046300; Serfor-Armah Y, 2001, WATER AIR SOIL POLL, V127, P243, DOI 10.1023/A:1005271005093; Smolders R, 2003, HUM ECOL RISK ASSESS, V9, P741, DOI 10.1080/713609965; Stengel DB, 2004, MAR POLLUT BULL, V48, P902, DOI 10.1016/j.marpolbul.2003.11.014; Vasconcelos MTSD, 2001, MAR CHEM, V74, P65, DOI 10.1016/S0304-4203(00)00096-7; Villares R, 2005, ESTUARIES, V28, P948, DOI 10.1007/BF02696022; Ye ZH, 2003, CHEMOSPHERE, V50, P795, DOI 10.1016/S0045-6535(02)00221-7; Yruela Inmaculada, 2005, Braz. J. Plant Physiol., V17, P145, DOI 10.1590/S1677-04202005000100012	57	12	12	1	33	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0963-9292			ECOTOXICOLOGY	Ecotoxicology	MAR	2012	21	2					591	600		10.1007/s10646-011-0819-6			10	Ecology; Environmental Sciences; Toxicology	Environmental Sciences & Ecology; Toxicology	891XU	WOS:000300251000028	22095160				2021-04-07	
J	Robinson, NM; Galicia-Garcia, C; Okolodkov, YB				Robinson, Nestor M.; Galicia-Garcia, Citlalli; Okolodkov, Yuri B.			NEW RECORDS OF GREEN (CHLOROPHYTA) AND BROWN ALGAE (PHAEOPHYCEAE) FOR CABEZO REEF, NATIONAL PARK SISTEMA ARRECIFAL VERACRUZANO, GULF OF MEXICO	ACTA BOTANICA MEXICANA			English	Article						anatomy; brown algae; green algae; Gulf of Mexico; new records; taxonomy		Descriptions of 13 green and 12 brown algal species from 20 genera and 15 families collected on Cabezo Reef of the National Park Sistema Arrecifal Veracruzano in the southwestern Gulf of Mexico, which are new records for the reef, are given. The species belong to the chlorophyte genera Caulerpa, Cladophoropsis, Codium, Dictyosphaeria, Ernodesmis, Halimeda, Neomeris, Parvocaulis, Percursaria and Rhipocephalus and the phaeophycean genera Canistrocarpus, Colpomenia, Cladosiphon, Dictyerpa, Dictyota, Ectocarpus, Padina, Rosenvingea, Sargassum and Sphacelaria. The family Dictyotaceae contained the largest number of species (6). The descriptions include morphometric and biological data and are accompanied by photographs and line drawings for each species. Data on the geographic distribution in the State of Veracruz, park and the Gulf of Mexico in general are also given. Percursaria percursa is a new record for the park, while Dictyerpa jamaicensis, Sargassum furcatum, Caulerpa racemosa var. occidentalis and Codium isthmocladum subsp. clavatum are new records for the State of Veracruz. Most of the specimens of Chlorophyta were found in the vegetative stage, whereas all the brown algal species except Dictyerpa jamaicensis and Sphacelaria rigidula possessed gametangia or sporangia.	[Okolodkov, Yuri B.] Univ Veracruzana, Inst Ciencias Marinas & Pesquerias, Boca Del Rio 94290, Veracruz, Mexico; [Robinson, Nestor M.; Galicia-Garcia, Citlalli] Inst Tecnol Boca del Rio, Biol Lab, Boca Del Rio 94290, Veracruz, Mexico	Okolodkov, YB (corresponding author), Univ Veracruzana, Inst Ciencias Marinas & Pesquerias, Calle Hidalgo Num 617, Boca Del Rio 94290, Veracruz, Mexico.	yuriokolodkov@yahoo.com	Robinson, Nestor/AAK-6481-2020	Robinson, Nestor M./0000-0001-7442-1666	Instituto de Ciencias Marinas y Pesquerias, Universidad Veracruzana (ICIMAP-UV); Programa de Mejoramiento del Profesorado	Our thanks to Captain Cipriano Anaya-Cruz for logistic support, Luz Elena Mateo-Cid and A. Catalina Mendoza-Gonzalez for their hospitality in the Laboratory of Phycology at Escuela Nacional de Ciencias Biologicas del Instituto Politecnico Nacional in Mexico City, Horacio Perez-Espa a from Instituto de Ciencias Marinas y Pesquerias, Universidad Veracruzana (ICIMAP-UV) for both logistic and financial support with the boat trip in 2010. The help of Sachico Hayasaka-Ramirez (ICIMAP-UV) in obtaining necessary literature is very much appreciated. Marcia M. Gowing from the University of California at Santa Cruz, California, USA, kindly improved the writing style. The present study was a part of the project of the Direccion General de Investigaciones de la Universidad Veracruzana "Algas de la zona arrecifal Veracruzana, Golfo de Mexico, con enfasis en las algas rojas, diatomeas y dinoflagelados" (2007-2009) given to YBO. Financial support of "Programa de Mejoramiento del Profesorado" to the project "Patrones de distribucion de la diversidad y biomasa de grupos funcionales clave para el Sistema Arrecifal Veracruzano" (2011-2012) is also appreciated.	Alves AM, 2010, HIDROBIOLOGICA, V20, P171; Bandeira-Pedrosa Maria Elizabeth, 2004, Braz. J. Bot., V27, P363, DOI 10.1590/S0100-84042004000200015; Boraxo de Zaixso A., 2004, HIST NAT SEG SERIE, V2, P95; Dawes C. J, 2008, SEAWWEEDS FLORIDA; Dreckmann K.M., 1998, CLASIFICACION NOMENC; Earle SA, 1969, PHYCOLOGIA, V7, P71, DOI 10.2216/i0031-8884-7-2-71.1; Flores-Moya Antonio, 1998, Acta Botanica Malacitana, V23, P197; Fredericq S., 2009, GULF MEXICO ORIGIN W, P187; Galicia Garcia C., 2007, INVESTIGACIONES CIEN, P141; Godínez-Ortega José Luis, 2009, TIP, V12, P59; Gonzalez-Gandara C., 2007, REV CIENTIFICA UDO A, V7, P252; Guiry G.M., 2011, ALGAEBASE; Joly A., 1967, GENEROS ALGAS MARINH; Lehman R. L, 1993, THESIS A M U TEXAS; Lehman R. L, 2007, ARRECIFES CORALINOS, P129; Littler DS., 2000, CARIBBEAN REEF PLANT; MENDOZA-GONZALEZ A. C., 2000, POLIBOTANICA, V11, P21; Moreira L, 2002, REV INVEST MAR, V23, P53; Norris J.N., 2010, MARINE ALGAE NO GULF; NORRIS JN, 1991, PHYCOLOGIA, V30, P315, DOI 10.2216/i0031-8884-30-4-315.1; Orduna-Medrano R. E., 2004, THESIS U VERACRUZANA; Ortega M. M., 2001, CATALOGO ALGAS BENTI; Schneider C.W., 1991, SEAWEEDS SE US; Silva PC, 1996, U CALIFORNIA PUBLICA, V79; Sole Maria A., 2003, Acta Botanica Venezuelica, V26, P41; Taylor WR, 1960, MARINE ALGAE E TROPI; VALENZUELA D. H., 1987, THESIS I POLITECNICO; Wynne MJ, 2011, NOVA HEDWIGIA, P7	28	7	11	0	5	INST ECOLOGIA AC	PATZCUARO	CENTRO REGIONAL DEL BAJIO, APARTADO POSTAL 386, PATZCUARO, MICHOACAN 61600, MEXICO	0187-7151			ACTA BOT MEX	Acta. Bot. Mex.		2012	101						11	48					38	Plant Sciences	Plant Sciences	017SI	WOS:000309610800002					2021-04-07	
J	Heinrich, S; Frickenhaus, S; Glockner, G; Valentin, K				Heinrich, Sandra; Frickenhaus, Stephan; Gloeckner, Gernot; Valentin, Klaus			A comprehensive cDNA library of light- and temperature-stressed Saccharina latissima (Phaeophyceae)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						cDNA library; EST; kelp; Laminaria saccharina; light acclimation; macroalgae; Phaeophyceae; Saccharina latissima; RNA extraction; temperature stress	EXPRESSED SEQUENCE TAGS; HEAT-SHOCK PROTEINS; LAMINARIA-SACCHARINA; BROWN-ALGAE; ULTRAVIOLET-RADIATION; UV-RADIATION; GENE ONTOLOGY; DNA-DAMAGE; BLUE-LIGHT; GROWTH	Macroalgae of the order Laminariales (kelps) are important marine coastal primary producers with prime significance for ecosystem function. Important factors influencing their distribution include light and temperature but the molecular basis of kelp responses to these factors is poorly understood. We therefore constructed a comprehensive cDNA library from RNA sampled under various light and temperature regimes, as a basis for future studies about the mechanisms and pathways involved in acclimation to light and temperature stress in Saccharina latissima. A total of 400 503 ESTs was assembled into 28 803 contigs. We were able to assign putative functions or orthology relationships to more than 10 000 contigs by BLASTx, Interpro protein-motif annotation, or Gene Ontology (GO). The most frequent Interpro protein domains found in the cDNA library were the protein kinase-like domain, serine/threonine-protein kinase-like domain, and NAD(P)-binding and thioredoxin-like fold domain. Enzyme code (EC) annotation yielded attributions for 480 contigs, providing a total of 625 ECs, which could be mapped to 85 biochemical pathways. Comparative genomics of S. latissima and Ectocarpus siliculosus indicated that our cDNA library gave a genome coverage of approximately 70%, assuming similar gene numbers in both species. GO term occurrence in S. latissima and E. siliculosus showed a similar distribution pattern among the root ontologies biological process, molecular function and cellular component. Comparative protein domain annotation of S. latissima und E. siliculosus showed that, probably due to the chosen stress conditions, the domains 'thioredoxin fold', 'thioredoxin-like fold', 'heat shock protein 70', and 'bromoperoxidase/chloroperoxidase C-terminal' are over-represented in the cDNA library.	[Heinrich, Sandra; Frickenhaus, Stephan; Valentin, Klaus] Alfred Wegener Inst Polar & Marine Res, D-27570 Bremerhaven, Germany; [Frickenhaus, Stephan] Hsch Bremerhaven, D-27568 Bremerhaven, Germany; [Gloeckner, Gernot] Inst Freshwater Ecol & Inland Fisheries, D-12587 Berlin, Germany	Heinrich, S (corresponding author), Alfred Wegener Inst Polar & Marine Res, Handelshafen 12, D-27570 Bremerhaven, Germany.	Sandra.Heinrich@awi.de	Valentin, Klaus/G-5862-2014; Glockner, Gernot/A-7800-2010	Valentin, Klaus/0000-0001-7401-9423; Glockner, Gernot/0000-0002-9061-1061; Frickenhaus, Stephan/0000-0002-0356-9791	PACES of the Alfred Wegener Institute, within the Helmholtz Foundation Initiative in Earth and Environment	We would like to thank Professor Dr Christian Wiencke for support. We also thank Dr Uwe John for participating in study design and providing lab space and Sylke Wohlrab for technical advice. This project was funded by the PACES research program of the Alfred Wegener Institute, within the Helmholtz Foundation Initiative in Earth and Environment.	Apprill AM, 2003, MAR ECOL PROG SER, V256, P75, DOI 10.3354/meps256075; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Arner ESJ, 2000, EUR J BIOCHEM, V267, P6102, DOI 10.1046/j.1432-1327.2000.01701.x; Ashburner M, 2000, NAT GENET, V25, P25, DOI 10.1038/75556; Balmer Y, 2004, P NATL ACAD SCI USA, V101, P2642, DOI 10.1073/pnas.0308583101; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; BOLTON JJ, 1983, PHYCOLOGIA, V22, P133, DOI 10.2216/i0031-8884-22-2-133.1; Borum J, 2002, MAR BIOL, V141, P11, DOI 10.1007/s00227-002-0806-9; Bouck A, 2007, MOL ECOL, V16, P907, DOI 10.1111/j.1365-294X.2006.03195.x; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Bruhn J, 1996, MAR BIOL, V125, P639, DOI 10.1007/BF00349245; Cai ZT, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-374; Carlsen BP, 2007, POLAR BIOL, V30, P939, DOI 10.1007/s00300-007-0272-4; Charpenteau M, 2004, BIOCHEM J, V379, P841, DOI 10.1042/BJ20031045; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Colin C, 2005, J BIOL INORG CHEM, V10, P156, DOI 10.1007/s00775-005-0626-8; Colin C, 2003, J BIOL CHEM, V278, P23545, DOI 10.1074/jbc.M300247200; Collen J, 2007, NEW PHYTOL, V176, P45, DOI 10.1111/j.1469-8137.2007.02152.x; Conesa A, 2005, BIOINFORMATICS, V21, P3674, DOI 10.1093/bioinformatics/bti610; Cosse A, 2009, NEW PHYTOL, V182, P239, DOI 10.1111/j.1469-8137.2008.02745.x; Crepineau F, 2000, PLANT MOL BIOL, V43, P503, DOI 10.1023/A:1006489920808; Davison IR, 2007, J PHYCOL, V43, P636, DOI 10.1111/j.1529-8817.2007.00360.x; DAVISON IR, 1991, MAR BIOL, V110, P449, DOI 10.1007/BF01344363; DAVISON IR, 1987, BRIT PHYCOL J, V22, P77, DOI 10.1080/00071618700650101; DAVISON IR, 1987, J PHYCOL, V23, P273, DOI 10.1111/j.1529-8817.1987.tb04135.x; De Martino A, 2000, EUR J BIOCHEM, V267, P5540, DOI 10.1046/j.1432-1327.2000.01616.x; DECKER CJ, 1995, CURR OPIN CELL BIOL, V7, P386, DOI 10.1016/0955-0674(95)80094-8; Devos D, 2000, PROTEINS, V41, P98, DOI 10.1002/1097-0134(20001001)41:1<98::AID-PROT120>3.0.CO;2-S; Dittami SM, 2011, BMC MOL BIOL, V12, DOI 10.1186/1471-2199-12-2; Fu WF, 2010, PLANT MOL BIOL REP, V28, P430, DOI 10.1007/s11105-009-0170-8; GERARD VA, 1988, MAR BIOL, V97, P25, DOI 10.1007/BF00391242; Goodwin KD, 1997, LIMNOL OCEANOGR, V42, P1725, DOI 10.4319/lo.1997.42.8.1725; Hardie DG, 1999, ANNU REV PLANT PHYS, V50, P97, DOI 10.1146/annurev.arplant.50.1.97; Holm M, 2001, EMBO J, V20, P118, DOI 10.1093/emboj/20.1.118; Hrabak EM, 2003, PLANT PHYSIOL, V132, P666, DOI 10.1104/pp.102.011999; Hu Z-L, 2008, ONLINE J BIOINFORMAT, V9, P108, DOI DOI 10.1186/1471-2105-13-134; Jimenez C, 2004, BBA-MOL CELL RES, V1644, P61, DOI 10.1016/j.bbamcr.2003.10.009; Kaul S, 2000, NATURE, V408, P796, DOI 10.1038/35048692; KIRST GO, 1995, J PHYCOL, V31, P181, DOI 10.1111/j.0022-3646.1995.00181.x; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; L?ning K., 1990, SEAWEEDS THEIR ENV B; La Barre S, 2010, MAR DRUGS, V8, P988, DOI 10.3390/md8040988; Lane CE, 2006, J PHYCOL, V42, P493, DOI 10.1111/j.1529-8817.2006.00204.x; Laturnus F, 1996, MAR CHEM, V55, P359, DOI 10.1016/S0304-4203(97)89401-7; Le Bail A, 2011, PLANT CELL, V23, P1666, DOI 10.1105/tpc.110.081919; Leblanc C, 2006, BIOCHIMIE, V88, P1773, DOI 10.1016/j.biochi.2006.09.001; Li D, 2001, CELL MOL LIFE SCI, V58, P2085, DOI 10.1007/PL00000838; LUNING K, 1980, J PHYCOL, V16, P1; LUNING K, 1975, MAR BIOL, V29, P195, DOI 10.1007/BF00391846; LUNING K, 1979, MAR ECOL PROG SER, V1, P195, DOI 10.3354/meps001195; LUNING K, 1972, PLANTA, V104, P252, DOI 10.1007/BF00387080; LUNING K, 1981, BER DEUT BOT GES, V94, P401; LUNING K, 1981, BRIT PHYCOL J, V16, P379; Machalek KM, 1996, PLANT CELL ENVIRON, V19, P1005, DOI 10.1111/j.1365-3040.1996.tb00207.x; Mardis ER, 2008, ANNU REV GENOM HUM G, V9, P387, DOI 10.1146/annurev.genom.9.081307.164359; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Mignone F, 2002, GENOME BIOL, V3, DOI 10.1186/gb-2002-3-3-reviews0004; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Pearson G, 2006, EUR J PHYCOL, V41, P97, DOI 10.1080/09670260500505011; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Pesole G, 2002, NUCLEIC ACIDS RES, V30, P335, DOI 10.1093/nar/30.1.335; Phillips N, 2011, AOB PLANTS, DOI 10.1093/aobpla/plr001; Quevillon E, 2005, NUCLEIC ACIDS RES, V33, pW116, DOI 10.1093/nar/gki442; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Roleda MY, 2007, PHOTOCHEM PHOTOBIOL, V83, P851, DOI 10.1562/2006-08-17-IR-1005; Roleda MY, 2006, MAR BIOL, V148, P1201, DOI 10.1007/s00227-005-0169-0; Roleda MY, 2006, PLANTA, V223, P407, DOI 10.1007/s00425-005-0092-0; Rousvoal S, 2011, PLANTA, V233, P261, DOI 10.1007/s00425-010-1295-6; Rudd S, 2003, TRENDS PLANT SCI, V8, P321, DOI 10.1016/S1360-1385(03)00131-6; Schurmann P, 2000, ANNU REV PLANT PHYS, V51, P371, DOI 10.1146/annurev.arplant.51.1.371; SHEFFIELD WP, 1990, J BIOL CHEM, V265, P11069; Spurkland T, 2011, BOT MAR, V54, P355, DOI 10.1515/BOT.2011.042; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Timperio AM, 2008, J PROTEOMICS, V71, P391, DOI 10.1016/j.jprot.2008.07.005; Varela-Alvarez E, 2006, J PHYCOL, V42, P741, DOI 10.1111/j.1529-8817.2006.00218.x; Waaland JR, 2004, J PHYCOL, V40, P26, DOI 10.1111/j.0022-3646.2003.03-148.x; Wang GG, 2005, J APPL PHYCOL, V17, P75, DOI 10.1007/s10811-005-5557-9; Wang WX, 2004, TRENDS PLANT SCI, V9, P244, DOI 10.1016/j.tplants.2004.03.006; Wheeler GL, 2008, GENETICS, V179, P193, DOI 10.1534/genetics.107.085936; Wiencke C, 2006, J ECOL, V94, P455, DOI 10.1111/j.1365-2745.2006.01102.x; Wiencke C, 2004, MAR BIOL, V145, P31, DOI 10.1007/s00227-004-1307-9; Wong TKM, 2007, J PHYCOL, V43, P528, DOI 10.1111/j.1529-8817.2007.00349.x	83	16	16	2	43	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0967-0262	1469-4433		EUR J PHYCOL	Eur. J. Phycol.		2012	47	2					83	94		10.1080/09670262.2012.660639			12	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	932WN	WOS:000303321300001					2021-04-07	
J	Evariste, E; Gachon, CMM; Callow, ME; Callow, JA				Evariste, Emmanuelle; Gachon, Claire M. M.; Callow, Maureen E.; Callow, James A.			Development and characteristics of an adhesion bioassay for ectocarpoid algae	BIOFOULING			English	Article						marine biofouling; brown algae; Ectocarpus; adhesion bioassays; Ulva	ULVA-LINZA ZOOSPORES; SETTLEMENT BEHAVIOR; DETACHMENT; STRENGTH; SURFACES; GROWTH; MODEL	Species of filamentous brown algae in the family Ectocarpaceae are significant members of fouling communities. However, there are few systematic studies on the influence of surface physico-chemical properties on their adhesion. In the present paper the development of a novel, laboratory-based adhesion bioassay for ectocarpoid algae, at an appropriate scale for the screening of sets of experimental samples in well-replicated and controlled experiments is described. The assays are based on the colonization of surfaces from a starting inoculum consisting of multicellular filaments obtained by blending the cultured alga Ectocarpus crouaniorum. The adhesion strength of the biomass after 14 days growth was assessed by applying a hydrodynamic shear stress. Results from adhesion tests on a set of standard surfaces showed that E. crouaniorum adhered more weakly to the amphiphilic Intersleek (R) 900 than to the more hydrophobic Intersleek (R) 700 and Silastic (R) T2 coatings. Adhesion to hydrophilic glass was also weak. Similar results were obtained for other cultivated species of Ectocarpus but differed from those obtained with the related ectocarpoid species Hincksia secunda. The response of the ectocarpoid algae to the surfaces was also compared to that for the green alga, Ulva.	[Evariste, Emmanuelle; Callow, Maureen E.; Callow, James A.] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England; [Gachon, Claire M. M.] Scottish Marine Inst, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland	Callow, JA (corresponding author), Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England.	j.a.callow@bham.ac.uk		Gachon, Claire/0000-0002-3702-7472	International Paint Ltd; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/J00460X/1, dml010007] Funding Source: researchfish	E. Evariste thanks International Paint Ltd for a studentship supporting these studies. The authors specifically thank Graeme Lyall, Kevin Reynolds, Lyndsey Tyson, David Stark, Adam Bell, Brent Tyson, David Williams, Gabrielle Prendergast, and Jennifer Longyear (International Paint Ltd) for advice and help in the preparation and characterization of coatings. The authors also thank Dr Jeremy Thomason (Marine Ecological Services) for advice on statistical analysis.	Amsler CD, 1999, J PHYCOL, V35, P239, DOI 10.1046/j.1529-8817.1999.3520239.x; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Bennett SM, 2010, BIOFOULING, V26, P235, DOI 10.1080/08927010903469676; Bowen J, 2007, J R SOC INTERFACE, V4, P473, DOI 10.1098/rsif.2006.0191; Briand JF, 2009, BIOFOULING, V25, P297, DOI 10.1080/08927010902745316; Callow JA, 2011, NAT COMMUN, V2, DOI 10.1038/ncomms1251; Callow ME, 1997, J PHYCOL, V33, P938, DOI 10.1111/j.0022-3646.1997.00938.x; Chaudhury MK, 2006, BIOINTERPHASES, V1, P18, DOI 10.1116/1.2188520; Coppejans E, 1995, MEISE BELGIUM JARDIN, P200; D'Souza F, 2010, J IND MICROBIOL BIOT, V37, P363, DOI 10.1007/s10295-009-0681-1; de Nys R, 2009, WOODHEAD PUBL MATER, P177, DOI 10.1533/9781845696313.1.177; Ederth T, 2008, BIOFOULING, V24, P303, DOI 10.1080/08927010802192650; Feinberg AW, 2003, ACS SYM SER, V838, P196; Finlay JA, 2008, BIOFOULING, V24, P219, DOI 10.1080/08927010802040693; Finnie A.A., 2010, BIOFOULING, P185, DOI 10.1002/97814 44315462.ch13.; Fletcher RL, 1980, CATALOGUE MAIN MARIN, V6, P7; GEISSLER U, 1983, NOVA HEDWIGIA, V37, P193; Greer SP, 2004, J PHYCOL, V40, P44, DOI 10.1111/j.0022-3646.2003.03-044.x; Greer SP, 2002, J PHYCOL, V38, P116, DOI 10.1046/j.1529-8817.2002.00178.x; HALL A, 1985, J MATER SCI, V20, P1111, DOI 10.1007/BF00585756; HALL A, 1980, NEW PHYTOL, V85, P73, DOI 10.1111/j.1469-8137.1980.tb04449.x; Holland R, 2004, BIOFOULING, V20, P323, DOI 10.1080/08927010400029031; JEFFREY SW, 1975, BIOCHEM PHYSIOL PFL, V167, P191, DOI 10.1016/s0015-3796(17)30778-3; Kavanagh CJ, 2005, J ADHESION, V81, P843, DOI 10.1080/00218460500189331; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lebret K, 2009, WOODHEAD PUBL MATER, P80, DOI 10.1533/9781845696313.1.80; Long CJ, 2010, BIOFOULING, V26, P411, DOI 10.1080/08927011003628849; Martinelli E, 2008, LANGMUIR, V24, P13138, DOI 10.1021/la801991k; Mineur F, 2007, MAR BIOL, V151, P1299, DOI 10.1007/s00227-006-0567-y; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; PYEFINCH KA, 1950, J ANIM ECOL, V19, P29, DOI 10.2307/1569; Quinn G.P., 2002, EXPT DESIGN DATA ANA; Schilp S, 2007, BIOINTERPHASES, V2, P143, DOI 10.1116/1.2806729; Schultz MP, 2011, BIOFOULING, V27, P87, DOI 10.1080/08927014.2010.542809; Schultz MP, 2007, BIOFOULING, V23, P331, DOI 10.1080/08927010701461974; Schultz MP, 2000, BIOFOULING, V15, P243, DOI 10.1080/08927010009386315; Schultz MP, 2003, BIOFOULING, V19, P17, DOI 10.1080/0892701031000089516; SHOAF WT, 1976, LIMNOL OCEANOGR, V21, P926, DOI 10.4319/lo.1976.21.6.0926; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Sundaram HS, 2011, BIOFOULING, V27, P589, DOI 10.1080/08927014.2011.587662; Swain GW, 1998, BIOFOULING, V12, P257, DOI 10.1080/08927019809378358; Thomas KV, 2010, BIOFOULING, V26, P73, DOI 10.1080/08927010903216564; Tribou M, 2010, BIOFOULING, V26, P47, DOI 10.1080/08927010903290973; West John A., 1996, Muelleria, V9, P29	44	6	7	1	28	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0892-7014	1029-2454		BIOFOULING	Biofouling		2012	28	1					15	27		10.1080/08927014.2011.643466			13	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	862GO	WOS:000298078000002	22146003				2021-04-07	
J	Owen, JR; Morris, CA; Nicolaus, B; Harwood, JL; Kille, P				Owen, Jennifer R.; Morris, Ceri A.; Nicolaus, Beate; Harwood, John L.; Kille, Peter			Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus	ECOTOXICOLOGY			English	Article						Phaeophyta; Copper; Toxicity; 14-3-3; Gene expression	BROWN ALGA; PROTEIN; PHOSPHORYLATION; 14-3-3-PROTEINS; CONTAMINATION; SERRATUS; DNA; LOCALIZATION; METABOLISM; INHIBITION	The macro-alga Fucus vesiculosus has a broad global and estuarine distribution and exhibits exceptional resistance to toxic metals, the molecular basis of which is poorly understood. To address this issue a cDNA library was constructed from an environmental isolate of F. vesiculosus growing in an area with chronic copper pollution. Characterisation of this library led to the identification of a cDNA encoding a protein known to be synthesised in response to toxicity, a full length 14-3-3 exhibiting a 71% identity to human/mouse epsilon isoform, 70-71% identity to yeast BMH1/2 and 95 and 71% identity to the Ectocarpus siliculosus 14-3-3 isoforms 1 and 2 respectively. Preliminary characterisation of the expression profile of the 14-3-3 indicated concentration- and time-dependent inductions on acute exposure of F. vesiculosus of copper (3-30 mu g/l). Higher concentrations of copper (a parts per thousand yen150 mu g/l) did not elicit significant induction of the 14-3-3 gene compared with the control even though levels of both intracellular copper and the expression of a cytosolic metal chaperone, metallothionein, continued to rise. Analysis of gene expression within environmental isolates demonstrated up-regulation of the 14-3-3 gene associated with the known copper pollution gradient. Here we report for the first time, identification of a gene encoding a putative 14-3-3 protein in a multicellular alga and provide preliminary evidence to link the induction of this 14-3-3 gene to copper exposure in this alga. Interestingly, the threshold exposure profile may be associated with a decrease in the organism's ability to control copper influx so that it perceives copper as a toxic response.	[Owen, Jennifer R.; Morris, Ceri A.; Nicolaus, Beate; Harwood, John L.; Kille, Peter] Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, S Glam, Wales	Kille, P (corresponding author), Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, S Glam, Wales.	Kille@cardiff.ac.uk	Harwood, John L/A-4308-2010; Kille, Peter/A-4337-2010	Kille, Peter/0000-0001-6023-5221; Harwood, John/0000-0003-2377-2612; Morris, Ceri/0000-0002-3293-5260	EERO; Natural and Environmental Research Council under the ROPA [GR3/R9694, GT04/99/MS/301]; NERCUK Research & Innovation (UKRI)NERC Natural Environment Research Council [GT5/94/ALS]	We would like to thank Dr Martijs Jonker (Microarray Department and Integrative Bioinformatics Unit, University of Amsterdam, The Netherlands) for his assistance in the statistical analysis of the data and Dr. Dennis Francis (Cardiff School of Biosciences, Cardiff University) for helpful discussions. This work was made possible by a number of different awards including an EERO Fellowship to Dr B. Nicolaus and two awards supported by the Natural and Environmental Research Council under the ROPA scheme (GR3/R9694) and a Ph.D studentship GT04/99/MS/301. The project supervision was facilitated by the NERC continued support for Dr Kille through the Advanced Fellowship program (GT5/94/ALS).	Adl SM, 2005, J EUKARYOT MICROBIOL, V52, P399, DOI 10.1111/j.1550-7408.2005.00053.x; AITKEN A, 1995, BIOCHEM SOC T, V23, P605, DOI 10.1042/bst0230605; Aksamit A, 2005, PLANT CELL PHYSIOL, V46, P1635, DOI 10.1093/pcp/pci179; Athwal GS, 2002, PLANT J, V29, P119, DOI 10.1046/j.0960-7412.2001.01200.x; Barreiro R, 2002, ENVIRON MONIT ASSESS, V75, P121, DOI 10.1023/A:1014479612811; Berg D, 2003, NAT REV NEUROSCI, V4, P752, DOI 10.1038/nrn1197; Brown MT, 2003, AQUAT TOXICOL, V64, P201, DOI 10.1016/S0166-445X(03)00054-7; Bryan GW, 1983, OCC PUBL MAR BIOL AS, V2, P1; Burridge TR, 2002, MAR POLLUT BULL, V45, P140, DOI 10.1016/S0025-326X(02)00126-1; Cairrao E, 2007, B ENVIRON CONTAM TOX, V79, P388, DOI 10.1007/s00128-007-9257-9; Chaseley J, 2003, THESIS CARDIFF U CAR; Cheng SH, 2002, PLANT PHYSIOL, V129, P469, DOI 10.1104/pp.005645; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Crescenzi KL, 1984, THESIS U WALES CARDI; Culotta VC, 1999, TRANSPORT INTRACELLU; Dameron CT, 1998, AM J CLIN NUTR, V67, p1091S, DOI 10.1093/ajcn/67.5.1091S; FERNANDES JC, 1991, BOT REV, V57, P246, DOI 10.1007/BF02858564; Garrick MD, 2003, BIOMETALS, V16, P41, DOI 10.1023/A:1020702213099; GELPERIN D, 1995, P NATL ACAD SCI USA, V92, P11539, DOI 10.1073/pnas.92.25.11539; Giusti L, 2001, ENVIRON INT, V26, P275, DOI 10.1016/S0160-4120(00)00117-3; Gordon AS, 2000, MAR CHEM, V70, P149, DOI 10.1016/S0304-4203(00)00019-0; Grotz N, 2006, BBA-MOL CELL RES, V1763, P595, DOI 10.1016/j.bbamcr.2006.05.014; Hall JL, 2003, J EXP BOT, V54, P2601, DOI 10.1093/jxb/erg303; Heredia J, 2001, J BIOL CHEM, V276, P8793, DOI 10.1074/jbc.M008179200; Himelblau E, 2000, CURR OPIN PLANT BIOL, V3, P205, DOI 10.1016/S1369-5266(00)00065-0; Huber SC, 2007, BIOCHEM SOC T, V35, P28, DOI 10.1042/BST0350028; KAREZ CS, 1995, BOT MAR, V38, P151, DOI 10.1515/botm.1995.38.1-6.151; Klychnikov OI, 2007, J EXP BOT, V58, P1013, DOI 10.1093/jxb/erl261; Kramer U, 2007, FEBS LETT, V581, P2263, DOI 10.1016/j.febslet.2007.04.010; Kumar S, 2008, BRIEF BIOINFORM, V9, P299, DOI 10.1093/bib/bbn017; Kupper H, 2002, J PHYCOL, V38, P429, DOI 10.1046/j.1529-8817.2002.01148.x; Larsen Annette K, 2003, Prog Cell Cycle Res, V5, P295; Martins LJ, 1998, J BIOL CHEM, V273, P23716, DOI 10.1074/jbc.273.37.23716; Merchant SS, 2006, BBA-MOL CELL RES, V1763, P578, DOI 10.1016/j.bbamcr.2006.04.007; Milton AH, 2006, EMBO J, V25, P1046, DOI 10.1038/sj.emboj.7600999; Morris CA, 1999, BIOCHEM J, V338, P553, DOI 10.1042/0264-6021:3380553; Nielsen HD, 2005, MAR POLLUT BULL, V51, P715, DOI 10.1016/j.marpolbul.2005.02.016; Nielsen HD, 2005, MAR POLLUT BULL, V50, P1675, DOI 10.1016/j.marpolbul.2005.07.004; Nielsen HD, 2003, NEW PHYTOL, V160, P157, DOI 10.1046/j.1469-8137.2003.00864.x; ORTIZ DF, 1995, J BIOL CHEM, V270, P4721, DOI 10.1074/jbc.270.9.4721; Palmgren MG, 1999, J EXP BOT, V50, P883, DOI 10.1093/jexbot/50.suppl_1.883; Palmgren MG, 2001, ANNU REV PLANT PHYS, V52, P817, DOI 10.1146/annurev.arplant.52.1.817; Paul AL, 2009, J PROTEOME RES, V8, P1913, DOI 10.1021/pr8008644; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; RAI LC, 1981, BIOL REV, V56, P99, DOI 10.1111/j.1469-185X.1981.tb00345.x; REED RH, 1990, HEAVY METAL TOLERANC; Roberts MR, 2003, TRENDS PLANT SCI, V8, P218, DOI 10.1016/S1360-1385(03)00056-6; Rosenquist M, 2000, J MOL EVOL, V51, P446, DOI 10.1007/s002390010107; Salgado LT, 2005, PROTOPLASMA, V225, P123, DOI 10.1007/s00709-004-0066-2; Sehnke PC, 2001, P NATL ACAD SCI USA, V98, P765, DOI 10.1073/pnas.021304198; SMITH KL, 1986, PHYSIOL PLANTARUM, V66, P692, DOI 10.1111/j.1399-3054.1986.tb05601.x; SMITH KL, 1982, PHYTOCHEMISTRY, V21, P569, DOI 10.1016/0031-9422(82)83142-7; SMITH KL, 1984, BIOCHIM BIOPHYS ACTA, V796, P119, DOI 10.1016/0005-2760(84)90245-5; Sokal RR, 1995, BIOMETRY PRINCIPLES, P589; Telles E, 2009, EXP CELL RES, V315, P1448, DOI 10.1016/j.yexcr.2009.01.018; Toth G, 2000, MAR ECOL PROG SER, V192, P119, DOI 10.3354/meps192119; Voskoboinik I, 2003, BIOCHEM BIOPH RES CO, V303, P337, DOI 10.1016/S0006-291X(03)00329-2; Wang YH, 2002, PLANT PHYSIOL, V130, P1361, DOI 10.1104/pp.008854; Weber CK, 2001, CANCER RES, V61, P3595; Yamazaki T, 2006, IN VIVO, V20, P605; Zhai QW, 2000, J CELL PHYSIOL, V184, P161, DOI 10.1002/1097-4652(200008)184:2<161::AID-JCP3>3.0.CO;2-N	61	12	12	1	20	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0963-9292	1573-3017		ECOTOXICOLOGY	Ecotoxicology	JAN	2012	21	1					124	138		10.1007/s10646-011-0772-4			15	Ecology; Environmental Sciences; Toxicology	Environmental Sciences & Ecology; Toxicology	874ZF	WOS:000298996900014	21909961				2021-04-07	
J	Chan, CX; Reyes-Prieto, A; Bhattacharya, D				Chan, Cheong Xin; Reyes-Prieto, Adrian; Bhattacharya, Debashish			Red and Green Algal Origin of Diatom Membrane Transporters: Insights into Environmental Adaptation and Cell Evolution	PLOS ONE			English	Article							HORIZONTAL GENE-TRANSFER; PHOTOSYNTHETIC EUKARYOTES; CYANIDIOSCHYZON-MEROLAE; GALDIERIA-SULPHURARIA; GENOME SEQUENCE; PROTEIN; REVEALS; TREE; DINOFLAGELLATE; ENDOSYMBIOSIS	Membrane transporters (MTs) facilitate the movement of molecules between cellular compartments. The evolutionary history of these key components of eukaryote genomes remains unclear. Many photosynthetic microbial eukaryotes (e. g., diatoms, haptophytes, and dinoflagellates) appear to have undergone serial endosymbiosis and thereby recruited foreign genes through endosymbiotic/horizontal gene transfer (E/HGT). Here we used the diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum as models to examine the evolutionary origin of MTs in this important group of marine primary producers. Using phylogenomics, we used 1,014 diatom MTs as query against a broadly sampled protein sequence database that includes novel genome data from the mesophilic red algae Porphyridium cruentum and Calliarthron tuberculosum, and the stramenopile Ectocarpus siliculosus. Our conservative approach resulted in 879 maximum likelihood trees of which 399 genes show a non-lineal history between diatoms and other eukaryotes and prokaryotes (at the bootstrap value >= 70%). Of the eukaryote-derived MTs, 172 (ca. 25% of 697 examined phylogenies) have members of both red/green algae as sister groups, with 103 putatively arising from green algae, 19 from red algae, and 50 have an unresolved affiliation to red and/or green algae. We used topology tests to analyze the most convincing cases of non-lineal gene history in which red and/or green algae were nested within stramenopiles. This analysis showed that ca. 6% of all trees (our most conservative estimate) support an algal origin of MTs in stramenopiles with the majority derived from green algae. Our findings demonstrate the complex evolutionary history of photosynthetic eukaryotes and indicate a reticulate origin of MT genes in diatoms. We postulate that the algal-derived MTs acquired via E/HGT provided diatoms and other related microbial eukaryotes the ability to persist under conditions of fluctuating ocean chemistry, likely contributing to their great success in marine environments.	Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08903 USA; Rutgers State Univ, Inst Marine & Coastal Sci, New Brunswick, NJ 08903 USA	Chan, CX (corresponding author), Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia.	bhattacharya@aesop.rutgers.edu	Chan, Cheong Xin/A-7005-2008	Chan, Cheong Xin/0000-0002-3729-8176	National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01-ES013679-01A2]; NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Environmental Health Sciences (NIEHS) [R01ES013679, R01ES013679, R01ES013679] Funding Source: NIH RePORTER	This work was partially supported by grant from the National Institutes of Health (R01-ES013679-01A2) to DB. We also acknowledge generous support from Rutgers University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Andersson JO, 2009, ANNU REV MICROBIOL, V63, P177, DOI 10.1146/annurev.micro.091208.073203; Archibald JM, 2003, P NATL ACAD SCI USA, V100, P7678, DOI 10.1073/pnas.1230951100; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Barbier G, 2005, PLANT PHYSIOL, V137, P460, DOI 10.1104/pp.104.051169; Baurain D, 2010, MOL BIOL EVOL, V27, P1698, DOI 10.1093/molbev/msq059; Bhattacharya D, 2004, BIOESSAYS, V26, P50, DOI 10.1002/bies.10376; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Burki F, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000790; Camacho C, 2009, BMC BIOINFORMATICS, V10, DOI 10.1186/1471-2105-10-421; Cavalier-Smith T, 1999, J EUKARYOT MICROBIOL, V46, P347, DOI 10.1111/j.1550-7408.1999.tb04614.x; CAVALIERSMITH T, 1986, PROGR PHYCOLOGICAL R, V14, P461; Chan CX, 2011, J BACTERIOL, V193, P3964, DOI 10.1128/JB.01524-10; Chan CX, 2011, CURR BIOL, V21, P328, DOI 10.1016/j.cub.2011.01.037; Chan CX, 2009, GENOME BIOL EVOL, V1, P429, DOI 10.1093/gbe/evp044; Chan CX, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0004524; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Conesa A, 2005, BIOINFORMATICS, V21, P3674, DOI 10.1093/bioinformatics/bti610; Dahl SG, 2004, J PHARMACOL EXP THER, V309, P853, DOI 10.1124/jpet.103.059972; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Elias M, 2009, BIOESSAYS, V31, P1273, DOI 10.1002/bies.200900117; Embley TM, 2006, NATURE, V440, P623, DOI 10.1038/nature04546; Frommolt R, 2008, MOL BIOL EVOL, V25, P2653, DOI 10.1093/molbev/msn206; Goldman N, 2000, SYST BIOL, V49, P652, DOI 10.1080/106351500750049752; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Hackett JD, 2007, MOL BIOL EVOL, V24, P1702, DOI 10.1093/molbev/msm089; Hackett Jeremiah D., 2008, P48; Hanikenne M, 2005, PLANT PHYSIOL, V137, P428, DOI 10.1104/pp.104.054189; Huysman MJJ, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-2-r17; Inagaki Y, 2006, P NATL ACAD SCI USA, V103, P4528, DOI 10.1073/pnas.0600744103; Janouskovec J, 2010, P NATL ACAD SCI USA, V107, P10949, DOI 10.1073/pnas.1003335107; Kamp A, 2011, P NATL ACAD SCI USA, V108, P5649, DOI 10.1073/pnas.1015744108; Kang LK, 2011, APPL ENVIRON MICROB, V77, P122, DOI 10.1128/AEM.01315-10; Keeling PJ, 2008, NAT REV GENET, V9, P605, DOI 10.1038/nrg2386; KISHINO H, 1989, J MOL EVOL, V29, P170, DOI 10.1007/BF02100115; Kramer U, 2007, FEBS LETT, V581, P2263, DOI 10.1016/j.febslet.2007.04.010; LaJeunesse TC, 2005, J PHYCOL, V41, P880, DOI 10.1111/j.0022-3646.2005.04231.x; Li SL, 2006, MOL BIOL EVOL, V23, P663, DOI 10.1093/molbev/msj075; Linka M, 2008, PLANT PHYSIOL, V148, P1487, DOI 10.1104/pp.108.129478; Martin W, 1998, PLANT PHYSIOL, V118, P9, DOI 10.1104/pp.118.1.9; Matsuzaki M, 2004, NATURE, V428, P653, DOI 10.1038/nature02398; Merchant S, 1998, ANNU REV PLANT PHYS, V49, P25, DOI 10.1146/annurev.arplant.49.1.25; Merchant SS, 2007, SCIENCE, V318, P245, DOI 10.1126/science.1143609; Moore RB, 2008, NATURE, V451, P959, DOI 10.1038/nature06635; Moustafa A, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-6; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; Niklas KJ, 2010, NEW PHYTOL, V185, P27, DOI 10.1111/j.1469-8137.2009.03054.x; Nikoh N, 2009, BMC BIOL, V7, DOI 10.1186/1741-7007-7-12; Nosenko T, 2007, BMC EVOL BIOL, V7, DOI 10.1186/1471-2148-7-173; Palenik B, 2006, P NATL ACAD SCI USA, V103, P13555, DOI 10.1073/pnas.0602963103; Paradis E, 2004, BIOINFORMATICS, V20, P289, DOI 10.1093/bioinformatics/btg412; Peers G, 2009, NATURE, V462, P518, DOI 10.1038/nature08587; Ren QH, 2007, NUCLEIC ACIDS RES, V35, pD274, DOI 10.1093/nar/gkl925; Reyes-Prieto A, 2008, CURR BIOL, V18, P956, DOI 10.1016/j.cub.2008.05.042; Rodriguez-Ezpeleta N, 2005, CURR BIOL, V15, P1325, DOI 10.1016/j.cub.2005.06.040; Rodriguez-Navarro A, 2006, J EXP BOT, V57, P1149, DOI 10.1093/jxb/erj068; Sanchez-Puerta MV, 2007, PROTIST, V158, P105, DOI 10.1016/j.protis.2006.09.004; Schmidt HA, 2002, BIOINFORMATICS, V18, P502, DOI 10.1093/bioinformatics/18.3.502; Scott C, 2008, NATURE, V452, P456, DOI 10.1038/nature06811; Shimodaira H, 1999, MOL BIOL EVOL, V16, P1114, DOI 10.1093/oxfordjournals.molbev.a026201; Shimodaira H, 2002, SYST BIOL, V51, P492, DOI 10.1080/10635150290069913; Stamatakis A, 2006, BIOINFORMATICS, V22, P2688, DOI 10.1093/bioinformatics/btl446; Strimmer K, 2002, P ROY SOC B-BIOL SCI, V269, P137, DOI 10.1098/rspb.2001.1862; Talavera G, 2007, SYST BIOL, V56, P564, DOI 10.1080/10635150701472164; Weber APM, 2006, EUKARYOT CELL, V5, P609, DOI 10.1128/EC.5.3.609-612.2006; Whelan S, 2001, MOL BIOL EVOL, V18, P691, DOI 10.1093/oxfordjournals.molbev.a003851; Worden AZ, 2009, SCIENCE, V324, P268, DOI 10.1126/science.1167222; YANG ZH, 1994, J MOL EVOL, V39, P306, DOI 10.1007/BF00160154; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075	68	30	30	0	36	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	DEC 14	2011	6	12							e29138	10.1371/journal.pone.0029138			11	Multidisciplinary Sciences	Science & Technology - Other Topics	866GS	WOS:000298369100161	22195008	DOAJ Gold, Green Published			2021-04-07	
J	Grenville-Briggs, L; Gachon, CMM; Strittmatter, M; Sterck, L; Kupper, FC; van West, P				Grenville-Briggs, Laura; Gachon, Claire M. M.; Strittmatter, Martina; Sterck, Lieven; Kuepper, Frithjof C.; van West, Pieter			A Molecular Insight into Algal-Oomycete Warfare: cDNA Analysis of Ectocarpus siliculosus Infected with the Basal Oomycete Eurychasma dicksonii	PLOS ONE			English	Article							LAMINARIA-DIGITATA PHAEOPHYCEAE; SAPROLEGNIA-PARASITICA; CHITIN SYNTHASE; GENOME SEQUENCE; PROTEINS; PATHOGEN; IDENTIFICATION; POTATO; GENE; MECHANISMS	Brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. Eurychasma dicksonii is an abundant, and probably cosmopolitan, obligate biotrophic oomycete pathogen of marine brown algae. Oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic Stramenopiles, mostly known for causing devastating agricultural and aquacultural diseases. Whilst molecular knowledge is restricted to crop pathogens, pathogenic oomycetes actually infect hosts from most eukaryotic lineages. Molecular evidence indicates that Eu. dicksonii belongs to the most early-branching oomycete clade known so far. Therefore Eu. dicksonii is of considerable interest due to its presumed environmental impact and phylogenetic position. Here we report the first large scale functional molecular data acquired on the most basal oomycete to date. 9873 unigenes, totalling over 3.5Mb of sequence data, were produced from Sanger-sequenced and pyrosequenced EST libraries of infected Ectocarpus siliculosus. 6787 unigenes (70%) were of algal origin, and 3086 (30%) oomycete origin. 57% of Eu. dicksonii sequences had no similarity to published sequence data, indicating that this dataset is largely unique. We were unable to positively identify sequences belonging to the RXLR and CRN groups of oomycete effectors identified in higher oomycetes, however we uncovered other unique pathogenicity factors. These included putative algal cell wall degrading enzymes, cell surface proteins, and cyclophilin-like proteins. A first look at the host response to infection has also revealed movement of the host nucleus to the site of infection as well as expression of genes responsible for strengthening the cell wall, and secretion of proteins such as protease inhibitors. We also found evidence of transcriptional reprogramming of E. siliculosus transposable elements and of a viral gene inserted in the host genome.	[Grenville-Briggs, Laura; Strittmatter, Martina; van West, Pieter] Univ Aberdeen, Aberdeen Oomycete Lab, Aberdeen, Scotland; [Gachon, Claire M. M.; Strittmatter, Martina; Kuepper, Frithjof C.] Scottish Marine Inst, Scottish Assoc Marine Sci, Oban, Argyll, Scotland; [Sterck, Lieven] Flanders Inst Biotechnol VIB, Dept Plant Syst Biol, Ghent, Belgium; [Sterck, Lieven] Univ Ghent, Dept Plant Biotechnol & Genet, B-9000 Ghent, Belgium	Grenville-Briggs, L (corresponding author), Univ Aberdeen, Aberdeen Oomycete Lab, Aberdeen, Scotland.	p.vanwest@abdn.ac.uk	Grenville-Briggs, Laura/B-3386-2013; Grenville-Briggs, Laura J/C-3419-2014; Sterck, Lieven/A-9439-2016	Grenville-Briggs, Laura J/0000-0001-5910-3651; Sterck, Lieven/0000-0001-7116-4000; Strittmatter, Martina/0000-0002-1258-9751; van West, Pieter/0000-0002-0767-6017; Kuepper, Frithjof/0000-0003-1273-7109; Gachon, Claire/0000-0002-3702-7472	University of Aberdeen; Biotechnology and Biological Sciences Research Council (BBSRC)UK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC); Natural Environment Research Council (NERC) via the SOFI initiativeUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/F012705/1]; New Investigator Grant [NE/D521522/1]; NERCUK Research & Innovation (UKRI)NERC Natural Environment Research Council [MGF 211]; European CommissionEuropean CommissionEuropean Commission Joint Research Centre [MIEF-CT-2006-022837]; European Reintegration Grant [PERG03-GA-2008-230865]; EUEuropean Commission [MEST-CT-2005-20501]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/F012578/1, NE/J00460X/1, NE/D521522/1] Funding Source: researchfish	This work is supported by the University of Aberdeen (PvW), the Biotechnology and Biological Sciences Research Council (BBSRC) (LGB, PvW), the Royal Society (PvW), the Total Foundation (FCK, PvW) and the Natural Environment Research Council (NERC) via the SOFI initiative (award NE/F012705/1) (LGB, CMMG, FCK and PvW), through Oceans 2025 WP4.5 (FCK), a New Investigator Grant (NE/D521522/1) (FCK) and a sequence allocation from the NERC Molecular Genetics Facility (Pilot Project MGF 211) (CMMG, PvW and FCK). The authors are also supported by the European Commission via a Marie Curie Intra-European Fellowship (MIEF-CT-2006-022837) (CMMG), a European Reintegration Grant (PERG03-GA-2008-230865) (CMMG), and an EU ECOSUMMER PhD fellowship (MEST-CT-2005-20501) (MS, FCK). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Badreddine I, 2008, EUKARYOT CELL, V7, P1980, DOI 10.1128/EC.00091-08; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; BARTNICK.S, 1968, ANNU REV MICROBIOL, V22, P87, DOI 10.1146/annurev.mi.22.100168.000511; Baxter L, 2010, SCIENCE, V330, P1549, DOI 10.1126/science.1195203; Berteau O, 2003, GLYCOBIOLOGY, V13, p29R, DOI 10.1093/glycob/cwg058; Bhattacharjee S, 2006, PLOS PATHOG, V2, P453, DOI 10.1371/journal.ppat.0020050; BULONE V, 1992, EXP MYCOL, V16, P8, DOI 10.1016/0147-5975(92)90037-R; Chou KC, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0009931; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Drummond AJ AB, 2011, GENEIOUS V4 8; Emanuelsson O, 2007, NAT PROTOC, V2, P953, DOI 10.1038/nprot.2007.131; Frada M, 2008, P NATL ACAD SCI USA, V105, P15944, DOI 10.1073/pnas.0807707105; Gachon CMM, 2010, TRENDS PLANT SCI, V15, P633, DOI 10.1016/j.tplants.2010.08.005; Gachon CMM, 2009, APPL ENVIRON MICROB, V75, P322, DOI 10.1128/AEM.01885-08; Gaulin E, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0001723; Gremme G, 2005, INFORM SOFTWARE TECH, V47, P965, DOI 10.1016/j.infsof.2005.09.005; Grenville-Briggs LJ, 2010, FUNGAL BIOL-UK, V114, P702, DOI 10.1016/j.funbio.2010.06.003; Grenville-Briggs LJ, 2005, ADV APPL MICROBIOL, V57, P217, DOI 10.1016/S0065-2164(05)57007-2; Grenville-Briggs LJ, 2005, FUNGAL GENET BIOL, V42, P244, DOI 10.1016/j.fgb.2004.11.009; Grouffaud S, 2008, MICROBIOL-SGM, V154, P3743, DOI 10.1099/mic.0.2008/021964-0; Grouffaud Severine, 2010, Fungal Biology Reviews, V24, P27, DOI 10.1016/j.fbr.2010.01.002; Guerriero G, 2010, PLOS PATHOG, V6, P1; Haas BJ, 2009, NATURE, V461, P393, DOI 10.1038/nature08358; Haverkort AJ, 2008, POTATO RES, V51, P47, DOI 10.1007/s11540-008-9089-y; Huang XQ, 1999, GENOME RES, V9, P868, DOI 10.1101/gr.9.9.868; Hunter S, 2009, NUCLEIC ACIDS RES, V37, pD211, DOI 10.1093/nar/gkn785; Kamoun S, 2003, EUKARYOT CELL, V2, P191, DOI 10.1128/EC.2.2.191-199.2003; KATSAROS C, 1992, PROTOPLASMA, V169, P75, DOI 10.1007/BF01343372; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Krogh A, 2001, J MOL BIOL, V305, P567, DOI 10.1006/jmbi.2000.4315; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Lafferty KD, 2008, ECOL LETT, V11, P533, DOI 10.1111/j.1461-0248.2008.01174.x; Larkin MA, 2007, BIOINFORMATICS, V23, P2947, DOI 10.1093/bioinformatics/btm404; Levesque CA, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-7-r73; Li H, 2006, NATURE, V442, P100; Marchler-Bauer A, 2011, NUCLEIC ACIDS RES, V39, pD225, DOI 10.1093/nar/gkq1189; Maumus F, 2009, THESIS U PARIS SUD; McLeod A, 2003, FUNGAL GENET BIOL, V38, P250, DOI 10.1016/S1087-1845(02)00523-6; Meijer HJG, 2006, MOL PLANT MICROBE IN, V19, P1348, DOI 10.1094/MPMI-19-1348; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Min XJ, 2005, NUCLEIC ACIDS RES, V33, pW677, DOI 10.1093/nar/gki394; MortBontemps M, 1997, MICROBIOL-UK, V143, P2009, DOI 10.1099/00221287-143-6-2009; Muller DG, 2008, CAH BIOL MAR, V49, P59; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; Oliva R, 2010, CELL MICROBIOL, V12, P705, DOI 10.1111/j.1462-5822.2010.01471.x; Pena PV, 2006, NATURE, V442, P100, DOI 10.1038/nature04814; Perez-Vilar J, 1999, J BIOL CHEM, V274, P31751, DOI 10.1074/jbc.274.45.31751; Phillips AJ, 2008, TRENDS MICROBIOL, V16, P13, DOI 10.1016/j.tim.2007.10.013; Rice P, 2000, TRENDS GENET, V16, P276, DOI 10.1016/S0168-9525(00)02024-2; Rocha EPC, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1001104; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES; Strittmatter M, 2009, OOMYCETE GENETICS GE, P25; Takemoto D, 2004, PLANT PHYSIOL, V136, P3864, DOI 10.1104/pp.104.052159; Taverna SD, 2006, MOL CELL, V24, P785, DOI 10.1016/j.molcel.2006.10.026; Tonon T, 2008, J PHYCOL, V44, P1250, DOI 10.1111/j.1529-8817.2008.00580.x; Tyler BM, 2006, SCIENCE, V313, P1261, DOI 10.1126/science.1128796; van West P, 2003, PHYSIOL MOL PLANT P, V62, P99, DOI 10.1016/S0885-5765(03)00044-4; Van West Pieter, 2006, Mycologist, V20, P99, DOI 10.1016/j.mycol.2006.06.004; van West P, 2010, FEMS MICROBIOL LETT, V310, P127, DOI 10.1111/j.1574-6968.2010.02055.x; Weinberger F, 2007, BIOL BULL-US, V213, P290, DOI 10.2307/25066646; West JA, 1999, HYDROBIOLOGIA, V399, P101; Whisson SC, 2007, NATURE, V450, P115, DOI 10.1038/nature06203; White ES, 2008, J PATHOL, V216, P1, DOI 10.1002/path.2388	67	24	25	0	22	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	SEP 15	2011	6	9							e24500	10.1371/journal.pone.0024500			14	Multidisciplinary Sciences	Science & Technology - Other Topics	822JR	WOS:000295041700035	21935414	DOAJ Gold, Green Published			2021-04-07	
J	Zalewska, T; Saniewski, M				Zalewska, Tamara; Saniewski, Michal			Bioaccumulation of Cs-137 by benthic plants and macroinvertebrates	OCEANOLOGICAL AND HYDROBIOLOGICAL STUDIES			English	Article						Radioactive cesium; marine phytobenthos; marine zoobenthos; bioaccumulation; Baltic Sea	MARINE MACROALGAE; BALTIC SEA; RADIONUCLIDES; RADIOACTIVITY; ACCUMULATION; ENVIRONMENTS; CESIUM-137; RETENTION; SR-90; WATER	Cs-137 activity concentrations were determined in macrophytes and macrozoobenthic organisms from the southern Baltic Sea. Cesium isotope content was analysed in macroalgae species (green, red and brown algae representatives) and in some species of vascular plants. The analyzed macroinvertebrate organisms included bivalves and a crustacean. Concentration factors (CF) were calculated using the determined Cs-137 concentration in the flora and fauna organisms against that in seawater, and the bioaccumulative properties were compared. The study pointed out that the most important factors in the cesium bioaccumulation process occurring in plants are related to morphology. The highest CF values were obtained in algae Polysiphonia fucoides, Ectocarpus siliculosus and Cladophora glomerata. Decidedly lower CF values were observed in the vascular plants and macrozoobenthos representatives.		Zalewska, T (corresponding author), Inst Meteorol & Water Management, Maritime Branch, Ul Waszyngtona 42, PL-81342 Gdynia, Poland.	tamara.zalewska@imgw.pl		Zalewska, Tamara/0000-0002-1030-8258; Saniewski, Michal/0000-0002-2347-1660			Boisson F, 1997, MAR POLLUT BULL, V35, P313, DOI 10.1016/S0025-326X(97)00092-1; Brown JE, 2006, MAR POLLUT BULL, V52, P1127, DOI 10.1016/j.marpolbul.2006.05.021; Burger J, 2006, J ENVIRON RADIOACTIV, V91, P27, DOI 10.1016/j.jenvrad.2006.08.003; CYBERSKA B, 1987, IMGW MAT ODDZIALU MO; HELCOM, 2003, BALT SEA ENV P, P5; *HELCOM, 1998, BALT SEA ENV P, V61, P59; HELCOM, 1997, MAN MAR MON COMBINE; HELCOM, 2009, BALT SEA ENV P, P47; IAEA, 2005, IAEATECDOC1429, P125; IAEA, 2010, IAEAAQ13; Ikaheimonen TK, 2009, J RADIOANAL NUCL CH, V282, P419, DOI 10.1007/s10967-009-0144-1; Knapinska-Skiba D., 1994, Netherlands Journal of Aquatic Ecology, V28, P413, DOI 10.1007/BF02334211; Knapinska-Skiba D., 1995, Netherlands Journal of Aquatic Ecology, V29, P283, DOI 10.1007/BF02084226; Knapinska-Skiba D, 2002, NUKLEONIKA, V47, P53; KNAPINSKASKIBA D, 2003, MAR POLLUT B, V46, P53; Kryshev AI, 2000, J ENVIRON RADIOACTIV, V50, P221, DOI 10.1016/S0265-931X(99)00118-6; Krzyminski W., 2000, IMGW MAT ODDZIALU MO; LITTLER MM, 1980, AM NAT, V116, P25, DOI 10.1086/283610; Lobban CS, 1997, SEAWEED ECOLOGY PHYS; Malek MA, 2004, J ENVIRON RADIOACTIV, V77, P191, DOI 10.1016/j.jenvrad.2004.03.006; Nielsen SP, 1999, SCI TOTAL ENVIRON, V238, P133, DOI 10.1016/S0048-9697(99)00130-8; Pinder JE, 2006, J ENVIRON RADIOACTIV, V85, P23, DOI 10.1016/j.jenvrad.2005.05.005; Sawidis T, 2003, ECOTOX ENVIRON SAFE, V54, P249, DOI 10.1016/S0147-6513(02)00021-0; Skwarzec B, 2003, J ENVIRON MONITOR, V5, P308, DOI 10.1039/b210341a; Smith JT, 2002, J ENVIRON RADIOACTIV, V62, P145, DOI 10.1016/S0265-931X(01)00157-6; Stengel DB, 2004, MAR POLLUT BULL, V48, P902, DOI 10.1016/j.marpolbul.2003.11.014; Szefer P, 2002, OCEANOLOGIA, V44, P129; Szefer P, 2002, METALS METALLOIDS RA, V5; Valkovic V., 2000, RADIOACTIVITY ENV; Zalewska T, 2006, J ENVIRON RADIOACTIV, V91, P1, DOI 10.1016/j.jenvrad.2006.08.001	30	11	11	0	20	VERSITA	WARSAW	SOLIPSKA 14A-1, 02-482 WARSAW, POLAND	1730-413X			OCEANOL HYDROBIOL ST	Oceanol. Hydrobiol. Stud.	SEP	2011	40	3					1	8		10.2478/s13545-011-0023-6			8	Marine & Freshwater Biology; Oceanography	Marine & Freshwater Biology; Oceanography	811QD	WOS:000294226900001					2021-04-07	
J	Maumus, F; Rabinowicz, P; Bowler, C; Rivarola, M				Maumus, Florian; Rabinowicz, Pablo; Bowler, Chris; Rivarola, Maximo			Stemming Epigenetics in Marine Stramenopiles	CURRENT GENOMICS			English	Article						Marine stramenopiles; epigenomics; DNA methylation; chromatin; diatom; genomics; Small RNA; Brown algae; Transposable elements	DIATOM PHAEODACTYLUM-TRICORNUTUM; ALGA ECTOCARPUS-SILICULOSUS; HORIZONTAL GENE-TRANSFER; RECEPTOR-LIKE KINASES; DNA METHYLATION; THALASSIOSIRA-PSEUDONANA; HISTONE H3; CYANIDIOSCHYZON-MEROLAE; TRANSPOSABLE ELEMENTS; GENOME SEQUENCES	Epigenetics include DNA methylation, the modification of histone tails that affect chromatin states, and small RNAs that are involved in the setting and maintenance of chromatin modifications. Marine stramenopiles (MAS), which are a diverse assemblage of algae that acquired photosynthesis from secondary endosymbiosis, include single-celled organisms such as diatoms as well as multicellular forms such as brown algae. The recent publication of two diatom genomes that diverged similar to 90 million years ago (mya), as well as the one of a brown algae that diverged from diatoms similar to 250 Mya, provide a great system of related, yet diverged set of organisms to compare epigenetic marks and their relationships. For example, putative DNA methyltransferase homologues were found in diatoms while none could be identified in the brown algal genome. On the other hand, no canonical DICER-like protein was found in diatoms in contrast to what is observed in brown algae. A key interest relies in understanding the adaptive nature of epigenetics and its inheritability. In contrast to yeast that lack DNA methylation, homogeneous cultures of diatoms constitute an attractive system to study epigenetic changes in response to environmental conditions such as nutrient-rich to nutrient-poor transitions which is especially relevant because of their ecological importance. P. tricornutum is also of outstanding interest because it is observed as three different morphotypes and thus constitutes a simple and promising model for the study of the epigenetic phenomena that accompany cellular differentiation. In this review we focus on the insights obtained from MAS comparative genomics and epigenomic analyses.	[Maumus, Florian] INRA Ctr Versailles Grignon, UR 1164, Unite Rech Genom Info, Versailles, France; [Rabinowicz, Pablo] Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Inst Genome Sci, Baltimore, MD 21201 USA; [Rivarola, Maximo] Inst Biotecnol CNIA, INTA, Buenos Aires, DF, Argentina; [Bowler, Chris] Ecole Normale Super, CNRS, UMR INSERM U1021 8197, Sect Genom Environm & Evolut,Inst Biol, Paris, France	Maumus, F (corresponding author), INRA Ctr Versailles Grignon, UR 1164, Unite Rech Genom Info, Versailles, France.	fmaumus@gmail.com; rivabros2@gmail.com	Bowler, Chris/AAC-6256-2019; Maumus, Florian/O-5426-2016; Rivarola, Maximo L/C-1261-2014	Maumus, Florian/0000-0001-7325-0527; Rivarola, Maximo/0000-0003-2818-1037; Bowler, Chris/0000-0003-3835-6187			Ahmed I, 2011, NUCL ACIDS RES; Allen AE, 2008, P NATL ACAD SCI USA, V105, P10438, DOI 10.1073/pnas.0711370105; Apt KE, 1996, MOL GEN GENET, V252, P572, DOI 10.1007/s004380050264; Archibald JM, 2007, BIOESSAYS, V29, P392, DOI 10.1002/bies.20551; Archibald JM, 2009, CURR BIOL, V19, pR81, DOI 10.1016/j.cub.2008.11.067; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Bailleul B, 2010, P NATL ACAD SCI USA, V107, P18214, DOI 10.1073/pnas.1007703107; Barakat TS, 2010, EXP CELL RES, V316, P679, DOI 10.1016/j.yexcr.2010.01.015; Barbier G, 2005, PLANT PHYSIOL, V137, P460, DOI 10.1104/pp.104.051169; Bird A, 2007, NATURE, V447, P396, DOI 10.1038/nature05913; Blouin NA, 2011, TRENDS PLANT SCI, V16, P29, DOI 10.1016/j.tplants.2010.10.004; Borsani O, 2005, CELL, V123, P1279, DOI 10.1016/j.cell.2005.11.035; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Bowler C, 2010, CURR OPIN PLANT BIOL, V13, P623, DOI 10.1016/j.pbi.2010.09.014; Bowler C, 2010, ANNU REV MAR SCI, V2, P333, DOI 10.1146/annurev-marine-120308-081051; Burki F, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000790; Carthew RW, 2009, CELL, V136, P642, DOI 10.1016/j.cell.2009.01.035; Cavalier-Smith T, 1999, J EUKARYOT MICROBIOL, V46, P347, DOI 10.1111/j.1550-7408.1999.tb04614.x; Cavalier-Smith T, 2010, BIOL LETTERS, V6, P342, DOI 10.1098/rsbl.2009.0948; Cerutti H, 2006, CURR GENET, V50, P81, DOI 10.1007/s00294-006-0078-x; Chang BS, 2007, SCIENCE, V318, P444, DOI 10.1126/science.1145801; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cock JM, 2002, CURR OPIN CELL BIOL, V14, P230, DOI 10.1016/S0955-0674(02)00305-8; Cokus SJ, 2008, NATURE, V452, P215, DOI 10.1038/nature06745; Connolly JA, 2008, J PHYCOL, V44, P124, DOI 10.1111/j.1529-8817.2007.00452.x; Corpet A, 2009, TRENDS CELL BIOL, V19, P29, DOI 10.1016/j.tcb.2008.10.002; Cubas P, 1999, NATURE, V401, P157, DOI 10.1038/43657; Cuperus JT, 2010, NAT STRUCT MOL BIOL, V17, P997, DOI 10.1038/nsmb.1866; Das C, 2009, NATURE, V459, P113, DOI 10.1038/nature07861; De Martino A, 2011, PROTIST, V162, P462, DOI 10.1016/j.protis.2011.02.002; De Martino A, 2009, BIOESSAYS, V31, P874, DOI 10.1002/bies.200900007; De Riso V, 2009, NUCLEIC ACIDS RES, V37, DOI 10.1093/nar/gkp448; De Smet I, 2009, NAT CELL BIOL, V11, P1166, DOI 10.1038/ncb1009-1166; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Falciatore A, 1999, MAR BIOTECHNOL, V1, P239, DOI 10.1007/PL00011773; Falkowski PG, 2004, SCIENCE, V305, P354, DOI 10.1126/science.1095964; Feng SH, 2010, SCIENCE, V330, P622, DOI 10.1126/science.1190614; Feng SH, 2010, P NATL ACAD SCI USA, V107, P8689, DOI 10.1073/pnas.1002720107; Flanagan JF, 2005, NATURE, V438, P1181, DOI 10.1038/nature04290; Flutre T, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0016526; GIBBS SP, 1993, ORIGINS OF PLASTIDS, P107; Gobler CJ, 2011, P NATL ACAD SCI USA, V108, P4352, DOI 10.1073/pnas.1016106108; Gogarten JP, 2005, NAT REV MICROBIOL, V3, P679, DOI 10.1038/nrmicro1204; Goll MG, 2005, ANNU REV BIOCHEM, V74, P481, DOI 10.1146/annurev.biochem.74.010904.153721; Goll MG, 2006, SCIENCE, V311, P395, DOI 10.1126/science.1120976; Hackett JD, 2007, MOL BIOL EVOL, V24, P1702, DOI 10.1093/molbev/msm089; Hawkes NA, 2002, J BIOL CHEM, V277, P3047, DOI 10.1074/jbc.M110445200; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Herr AJ, 2005, SCIENCE, V308, P118, DOI 10.1126/science.1106910; HOLLIDAY R, 1975, SCIENCE, V187, P226, DOI 10.1126/science.1111098; Huson DH, 2006, MOL BIOL EVOL, V23, P254, DOI 10.1093/molbev/msj030; JARVIS EE, 1992, J PHYCOL, V28, P356, DOI 10.1111/j.0022-3646.1992.00356.x; Jeddeloh JA, 1999, NAT GENET, V22, P94, DOI 10.1038/8803; Keeling PJ, 2008, NAT REV GENET, V9, P605, DOI 10.1038/nrg2386; Kim J, 2006, EMBO REP, V7, P397, DOI 10.1038/sj.embor.7400625; KIYOTA E, J PLANT RES; Kohler C, 2010, HEREDITY, V105, P57, DOI 10.1038/hdy.2009.176; Kooistra WHCF, 1996, MOL PHYLOGENET EVOL, V6, P391, DOI 10.1006/mpev.1996.0088; Kouzarides T, 2007, CELL, V128, P693, DOI 10.1016/j.cell.2007.02.005; Lister R, 2009, NATURE, V462, P315, DOI 10.1038/nature08514; Luco RF, 2010, SCIENCE, V327, P996, DOI 10.1126/science.1184208; MacRae IJ, 2006, SCIENCE, V311, P195, DOI 10.1126/science.1121638; Maheswari U, 2009, NUCLEIC ACIDS RES, V37, pD1001, DOI 10.1093/nar/gkn905; Matsuzaki M, 2004, NATURE, V428, P653, DOI 10.1038/nature02398; Maumus F, 2009, BMC GENOMICS, V10, DOI 10.1186/1471-2164-10-624; Miura A, 2001, NATURE, V411, P212, DOI 10.1038/35075612; Mochizuki K, 2005, GENE DEV, V19, P77, DOI 10.1101/gad.1265105; Mock T, 2008, P NATL ACAD SCI USA, V105, P1579, DOI 10.1073/pnas.0707946105; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; Myant K, 2008, MOL CELL BIOL, V28, P215, DOI 10.1128/MCB.01073-07; NELSON DM, 1995, GLOBAL BIOGEOCHEM CY, V9, P359, DOI 10.1029/95GB01070; Nowrousian M, 2010, EUKARYOT CELL, V9, P1300, DOI 10.1128/EC.00123-10; Okamoto N, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0007080; Park PJ, 2009, NAT REV GENET, V10, P669, DOI 10.1038/nrg2641; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Ponger L, 2005, MOL BIOL EVOL, V22, P1119, DOI 10.1093/molbev/msi098; Pradhan S, 1999, J BIOL CHEM, V274, P33002, DOI 10.1074/jbc.274.46.33002; Quesneville H, 2005, PLOS COMPUT BIOL, V1, P166, DOI 10.1371/journal.pcbi.0010022; Rajagopalan R, 2006, GENE DEV, V20, P3407, DOI 10.1101/gad.1476406; Raven JA, 2004, NEW PHYTOL, V162, P45, DOI 10.1111/j.1469-8137.2004.01022.x; Rayko E, 2010, NEW PHYTOL, V188, P52, DOI 10.1111/j.1469-8137.2010.03371.x; RIGGS AD, 1975, CYTOGENET CELL GENET, V14, P9, DOI 10.1159/000130315; Rogakou EP, 1999, J CELL BIOL, V146, P905, DOI 10.1083/jcb.146.5.905; Roudier F, 2009, TRENDS GENET, V25, P511, DOI 10.1016/j.tig.2009.09.013; Round F.E., 1990, DIATOMS BIOL MORPHOL; Roy SW, 2007, MOL BIOL EVOL, V24, P1447, DOI 10.1093/molbev/msm048; Shi HF, 2006, RNA, V12, P2063, DOI 10.1261/rna.246906; Shiu SH, 2001, P NATL ACAD SCI USA, V98, P10763, DOI 10.1073/pnas.181141598; Siaut M, 2007, GENE, V406, P23, DOI 10.1016/j.gene.2007.05.022; Slotkin RK, 2007, NAT REV GENET, V8, P272, DOI 10.1038/nrg2072; Tachibana M, 2001, J BIOL CHEM, V276, P25309, DOI 10.1074/jbc.M101914200; Taipale M, 2005, MOL CELL BIOL, V25, P6798, DOI 10.1128/MCB.25.15.6798-6810.2005; Teixeira FK, 2010, HEREDITY, V105, P14, DOI 10.1038/hdy.2010.52; Teixeira FK, 2009, SCIENCE, V323, P1600, DOI 10.1126/science.1165313; Trojer P, 2007, CELL, V129, P915, DOI 10.1016/j.cell.2007.03.048; Vance V, 2001, SCIENCE, V292, P2277, DOI 10.1126/science.1061334; VAUCHERET H, 2005, SCI STKE, pPE43, DOI DOI 10.1126/STKE.3002005PE42; Verreault A, 1998, CURR BIOL, V8, P96, DOI 10.1016/S0960-9822(98)70040-5; Voinnet O, 2009, CELL, V136, P669, DOI 10.1016/j.cell.2009.01.046; Voorhoeve PM, 2003, TRENDS BIOTECHNOL, V21, P2, DOI 10.1016/S0167-7799(02)00002-1; Wang LA, 1997, MOL CELL BIOL, V17, P519, DOI 10.1128/MCB.17.1.519; Wang Z, 2009, NAT REV GENET, V10, P57, DOI 10.1038/nrg2484; WASSENEGGER M, 1994, CELL, V76, P567, DOI 10.1016/0092-8674(94)90119-8; Wilkinson LS, 2007, NAT REV NEUROSCI, V8, P832, DOI 10.1038/nrn2235; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zemach A, 2010, SCIENCE, V328, P916, DOI 10.1126/science.1186366; Zofall M, 2006, MOL CELL, V22, P681, DOI 10.1016/j.molcel.2006.05.010	107	16	16	2	29	BENTHAM SCIENCE PUBL LTD	SHARJAH	EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES	1389-2029	1875-5488		CURR GENOMICS	Curr. Genomics	AUG	2011	12	5					357	370		10.2174/138920211796429727			14	Biochemistry & Molecular Biology; Genetics & Heredity	Biochemistry & Molecular Biology; Genetics & Heredity	806XK	WOS:000293850600005	22294878	Green Published			2021-04-07	
J	Coelho, SM; Godfroy, O; Arun, A; Le Corguille, G; Peters, AF; Cock, JM				Coelho, Susana M.; Godfroy, Olivier; Arun, Alok; Le Corguille, Gildas; Peters, Akira F.; Cock, J. Mark			OUROBOROS is a master regulator of the gametophyte to sporophyte life cycle transition in the brown alga Ectocarpus	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA			English	Article						brown algae; development; phaeophyceae; stramenopiles	LAND PLANT EVOLUTION; SEED DEVELOPMENT; SILICULOSUS PHAEOPHYCEAE; ENDOSPERM DEVELOPMENT; ARABIDOPSIS-THALIANA; FERTILIZATION; APOMIXIS; COMPLEX; GENOME; EUKARYOTES	The brown alga Ectocarpus siliculosus has a haploid-diploid life cycle that involves an alternation between two distinct generations, the sporophyte and the gametophyte. We describe a mutant, ouroboros (oro), in which the sporophyte generation is converted into a functional, gamete-producinggametophyte. The life history of the mutant thus consists of a continuous reiteration of the gametophyte generation. The oro mutant exhibited morphological features typical of the gametophyte generation and accumulated transcripts of gametophyte generation marker genes. Genetic analysis showed that oro behaved as a single, recessive, Mendelian locus that was unlinked to the IMMEDIATE UPRIGHT locus, which has been shown to be necessary for full expression of the sporophyte developmental program. The data presented here indicate that ORO is a master regulator of the gametophyte-to-sporophyte life cycle transition and, moreover, that oro represents a unique class of homeotic mutation that results in switching between two developmental programs that operate at the level of the whole organism.	[Coelho, Susana M.; Godfroy, Olivier; Arun, Alok; Cock, J. Mark] CNRS, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, Unite Mixte Rech 7139, F-29682 Roscoff, France; [Coelho, Susana M.; Godfroy, Olivier; Arun, Alok; Cock, J. Mark] Univ Paris 06, Marine Plants & Biomol Lab, Stn Biol Roscoff, Unite Mixte Rech 7139, F-29682 Roscoff, France; [Le Corguille, Gildas] Stn Biol Roscoff, Serv Informat & Genom, F-29682 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29250 Santec, France	Cock, JM (corresponding author), CNRS, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, Unite Mixte Rech 7139, F-29682 Roscoff, France.	cock@sb-roscoff.fr	Arun, Alok/AAJ-7923-2020; Coelho, Susana/ABH-8166-2020	Arun, Alok/0000-0003-4666-9802; Cock, J. Mark/0000-0002-2650-0383	Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); Universite Pierre et Marie Curie; Groupement d'Interet Scientifique Genomique Marine; Interreg program France (Channel)-England; Agence Nationale de la RechercheFrench National Research Agency (ANR)European Commission; European Erasmus Mundus Program Fellowship	We thank Delphine Scornet, Guillaume Hatte, and Laurence Dartevelle for technical assistance. This work was supported by Centre National de la Recherche Scientifique, Universite Pierre et Marie Curie, Groupement d'Interet Scientifique Genomique Marine, the Interreg program France (Channel)-England (project Marinexus), and Agence Nationale de la Recherche (Project Bi-cycle). A. A. was supported by a European Erasmus Mundus Program Fellowship.	Bicknell RA, 2004, PLANT CELL, V16, pS228, DOI 10.1105/tpc.017921; Bothwell JH, 2010, NEW PHYTOL, V188, P111, DOI 10.1111/j.1469-8137.2010.03357.x; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Chaudhury AM, 1997, P NATL ACAD SCI USA, V94, P4223, DOI 10.1073/pnas.94.8.4223; Cock JM, 2010, NEW PHYTOL, V188, P1, DOI 10.1111/j.1469-8137.2010.03454.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Ebel C, 2004, NATURE, V429, P776, DOI 10.1038/nature02637; Guitton AE, 2004, DEVELOPMENT, V131, P2971, DOI 10.1242/dev.01168; Guitton AE, 2005, CURR BIOL, V15, P750, DOI 10.1016/j.cub.2005.02.066; Khurana JS, 2010, J CELL BIOL, V191, P905, DOI 10.1083/jcb.201006034; Kohler C, 2003, EMBO J, V22, P4804, DOI 10.1093/emboj/cdg444; Koltunow AM, 2003, ANNU REV PLANT BIOL, V54, P547, DOI 10.1146/annurev.arplant.54.110901.160842; Mosquna A, 2009, DEVELOPMENT, V136, P2433, DOI 10.1242/dev.035048; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1970, NATURWISSENSCHAFTEN, V57, P357; MULLER DG, 1975, ARCH PROTISTENKD, V117, P297; Ohad N, 1999, PLANT CELL, V11, P407, DOI 10.1105/tpc.11.3.407; Ohad N, 1996, P NATL ACAD SCI USA, V93, P5319, DOI 10.1073/pnas.93.11.5319; Okano Y, 2009, P NATL ACAD SCI USA, V106, P16321, DOI 10.1073/pnas.0906997106; Ozias-Akins P, 2007, ANNU REV GENET, V41, P509, DOI 10.1146/annurev.genet.40.110405.090511; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Thornber CS, 2006, INTEGR COMP BIOL, V46, P605, DOI 10.1093/icb/icl018; Tusher VG, 2001, P NATL ACAD SCI USA, V98, P5116, DOI 10.1073/pnas.091062498; Wuest SE, 2010, CURR BIOL, V20, P506, DOI 10.1016/j.cub.2010.01.051; Yamagishi T, 2004, PLANTA, V219, P253, DOI 10.1007/s00425-004-1230-9	30	58	58	0	15	NATL ACAD SCIENCES	WASHINGTON	2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA	0027-8424			P NATL ACAD SCI USA	Proc. Natl. Acad. Sci. U. S. A.	JUL 12	2011	108	28					11518	11523		10.1073/pnas.1102274108			6	Multidisciplinary Sciences	Science & Technology - Other Topics	791BO	WOS:000292635200047	21709217	Green Published, Bronze			2021-04-07	
J	Delage, L; Leblanc, C; Collen, PN; Gschloessl, B; Oudot, MP; Sterck, L; Poulain, J; Aury, JM; Cock, JM				Delage, Ludovic; Leblanc, Catherine; Collen, Pi Nyvall; Gschloessl, Bernhard; Oudot, Marie-Pierre; Sterck, Lieven; Poulain, Julie; Aury, Jean-Marc; Cock, J. Mark			In Silico Survey of the Mitochondrial Protein Uptake and Maturation Systems in the Brown Alga Ectocarpus siliculosus	PLOS ONE			English	Article							OUTER-MEMBRANE BIOGENESIS; DISULFIDE BOND FORMATION; AMINO-ACID-SEQUENCE; INNER-MEMBRANE; SACCHAROMYCES-CEREVISIAE; INTERMEMBRANE SPACE; PRECURSOR PROTEINS; SUBCELLULAR-LOCALIZATION; PROCESSING PEPTIDASE; YEAST MITOCHONDRIA	The acquisition of mitochondria was a key event in eukaryote evolution. The aim of this study was to identify homologues of the components of the mitochondrial protein import machinery in the brown alga Ectocarpus and to use this information to investigate the evolutionary history of this fundamental cellular process. Detailed searches were carried out both for components of the protein import system and for related peptidases. Comparative and phylogenetic analyses were used to investigate the evolution of mitochondrial proteins during eukaryote diversification. Key observations include phylogenetic evidence for very ancient origins for many protein import components (Tim21, Tim50, for example) and indications of differences between the outer membrane receptors that recognize the mitochondrial targeting signals, suggesting replacement, rearrangement and/or emergence of new components across the major eukaryotic lineages. Overall, the mitochondrial protein import components analysed in this study confirmed a high level of conservation during evolution, indicating that most are derived from very ancient, ancestral proteins. Several of the protein import components identified in Ectocarpus, such as Tim21, Tim50 and metaxin, have also been found in other stramenopiles and this study suggests an early origin during the evolution of the eukaryotes.	[Delage, Ludovic; Leblanc, Catherine; Collen, Pi Nyvall; Gschloessl, Bernhard; Cock, J. Mark] Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, Roscoff, France; [Delage, Ludovic; Leblanc, Catherine; Collen, Pi Nyvall; Gschloessl, Bernhard; Cock, J. Mark] CNRS, UMR 7139, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, Roscoff, France; [Oudot, Marie-Pierre] Univ British Columbia, Dept Bot, Vancouver, BC, Canada; [Sterck, Lieven] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium; [Poulain, Julie; Aury, Jean-Marc] Commissariat Energie Atom, Direct Sci Vivant, Inst Genom, Evry, France; [Poulain, Julie; Aury, Jean-Marc] CNRS, UMR 8030, Evry, France; [Poulain, Julie; Aury, Jean-Marc] Univ Evry, Evry, France	Delage, L (corresponding author), Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, Roscoff, France.	cock@sb-roscoff.fr	Aury, Jean-Marc/N-1621-2019; Sterck, Lieven/A-9439-2016	Aury, Jean-Marc/0000-0003-1718-3010; Sterck, Lieven/0000-0001-7116-4000; Cock, J. Mark/0000-0002-2650-0383; Poulain, Julie/0000-0002-8744-3116	French GIS "Institut de la Genomique Marine"; Centre National de Recherche Scientifique; European Union network of excellence Marine Genomics Europe; GIS Europole Mer; University Pierre and Marie Curie	This work was supported by the French GIS "Institut de la Genomique Marine", the Centre National de Recherche Scientifique, the European Union network of excellence Marine Genomics Europe, the GIS Europole Mer, the University Pierre and Marie Curie. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	Abe Y, 2000, CELL, V100, P551, DOI 10.1016/S0092-8674(00)80691-1; Allen JWA, 2008, FEBS LETT, V582, P2817, DOI 10.1016/j.febslet.2008.07.015; Arnold K, 2006, BIOINFORMATICS, V22, P195, DOI 10.1093/bioinformatics/bti770; Bannai H, 2002, BIOINFORMATICS, V18, P298, DOI 10.1093/bioinformatics/18.2.298; Berks BC, 2005, CURR OPIN MICROBIOL, V8, P174, DOI 10.1016/j.mib.2005.02.010; Berks BC, 1996, MOL MICROBIOL, V22, P393, DOI 10.1046/j.1365-2958.1996.00114.x; Biegert A, 2008, BIOINFORMATICS, V24, P807, DOI 10.1093/bioinformatics/btn039; Bogsch EG, 1998, J BIOL CHEM, V273, P18003, DOI 10.1074/jbc.273.29.18003; Bolliger L, 1995, EMBO J, V14, P6318, DOI 10.1002/j.1460-2075.1995.tb00322.x; Braun HP, 1997, INT J BIOCHEM CELL B, V29, P1043, DOI 10.1016/S1357-2725(97)00032-0; Burri L, 2006, FEBS J, V273, P1507, DOI 10.1111/j.1742-4658.2006.05171.x; Burri L, 2005, MOL BIOL CELL, V16, P2926, DOI 10.1091/mbc.E04-12-1086; Calvo S, 2006, NAT GENET, V38, P576, DOI 10.1038/ng1776; Chacinska A, 2009, CELL, V138, P628, DOI 10.1016/j.cell.2009.08.005; Chan NC, 2008, MOL BIOL CELL, V19, P126, DOI 10.1091/mbc.E07-08-0796; Chan NC, 2006, J MOL BIOL, V358, P1010, DOI 10.1016/j.jmb.2006.02.062; Chen H, 2006, BIOINFORMATICS, V22, P376, DOI 10.1093/bioinformatics/bti822; Chew O, 2004, FEBS LETT, V557, P109, DOI 10.1016/S0014-5793(03)01457-1; Claros MG, 1996, EUR J BIOCHEM, V241, P779, DOI 10.1111/j.1432-1033.1996.00779.x; Cline K, 2001, J CELL BIOL, V154, P719, DOI 10.1083/jcb.200105149; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Cserzo M, 1997, PROTEIN ENG, V10, P673, DOI 10.1093/protein/10.6.673; de Castro E, 2006, NUCLEIC ACIDS RES, V34, pW362, DOI 10.1093/nar/gkl124; de Marcos-Lousa C, 2006, TRENDS BIOCHEM SCI, V31, P259, DOI 10.1016/j.tibs.2006.03.006; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Dereeper A, 2008, NUCLEIC ACIDS RES, V36, pW465, DOI 10.1093/nar/gkn180; Dinur-Mills M, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002161; Dolezal P, 2006, SCIENCE, V313, P314, DOI 10.1126/science.1127895; Emanuelsson O, 2000, J MOL BIOL, V300, P1005, DOI 10.1006/jmbi.2000.3903; Esser K, 2004, MOL GENET GENOMICS, V271, P616, DOI 10.1007/s00438-004-1011-y; Figueroa-Martinez F, 2008, GENETICS, V179, P149, DOI 10.1534/genetics.108.087965; Finn RD, 2010, NUCLEIC ACIDS RES, V38, pD211, DOI 10.1093/nar/gkp985; Funes S, 2004, MOL BIOL CELL, V15, P1853, DOI 10.1091/mbc.E03-11-0789; Funes S, 2009, P NATL ACAD SCI USA, V106, P6656, DOI 10.1073/pnas.0809951106; Gabaldon T, 2003, SCIENCE, V301, P609, DOI 10.1126/science.1085463; Gakh E, 2002, BBA-MOL CELL RES, V1592, P63, DOI 10.1016/S0167-4889(02)00265-3; Gaston D, 2009, METHOD ENZYMOL, V457, P21, DOI 10.1016/S0076-6879(09)05002-2; Gatsos X, 2008, FEMS MICROBIOL REV, V32, P995, DOI 10.1111/j.1574-6976.2008.00130.x; Gentle I, 2004, J CELL BIOL, V164, P19, DOI 10.1083/jcb.200310092; Gentle IE, 2007, MOL BIOL EVOL, V24, P1149, DOI 10.1093/molbev/msm031; Geourjon C, 1995, COMPUT APPL BIOSCI, V11, P681; Glaser E, 1998, PLANT MOL BIOL, V38, P311, DOI 10.1023/A:1006020208140; Glaser E, 1999, J BIOENERG BIOMEMBR, V31, P259, DOI 10.1023/A:1005475930477; Gray MW, 2001, GENOME BIOL, V2; Gray MW, 1999, CURR OPIN GENET DEV, V9, P678, DOI 10.1016/S0959-437X(99)00030-1; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Guda C, 2004, NUCLEIC ACIDS RES, V32, pW372, DOI 10.1093/nar/gkh374; HAHNE K, 1994, CELL, V79, P829, DOI 10.1016/0092-8674(94)90072-8; HARTL FU, 1989, BIOCHIM BIOPHYS ACTA, V988, P1, DOI 10.1016/0304-4157(89)90002-6; Hawkins John, 2006, Journal of Bioinformatics and Computational Biology, V4, P1, DOI 10.1142/S0219720006001771; HAWLITSCHEK G, 1988, CELL, V53, P795, DOI 10.1016/0092-8674(88)90096-7; Herlan M, 2003, J BIOL CHEM, V278, P27781, DOI 10.1074/jbc.M211311200; Herrmann JM, 2009, BBA-MOL CELL RES, V1793, P71, DOI 10.1016/j.bbamcr.2008.05.001; Hofhaus G, 2003, EUR J BIOCHEM, V270, P1528, DOI 10.1046/j.1432-1033.2003.03519.x; Hofmann K., 1993, BIOL CHEM, V374, P166; Hoppins SC, 2004, J BIOL CHEM, V279, P12396, DOI 10.1074/jbc.M313037200; ISAYA G, 1992, J BIOL CHEM, V267, P7904; Jan PS, 2000, MOL GEN GENET, V263, P483, DOI 10.1007/s004380051192; Juhola MK, 2000, FEBS LETT, V481, P91, DOI 10.1016/S0014-5793(00)01989-X; Karpenahalli MR, 2007, BMC BIOINFORMATICS, V8, DOI 10.1186/1471-2105-8-2; Kelley LA, 2009, NAT PROTOC, V4, P363, DOI 10.1038/nprot.2009.2; Kitada S, 2007, J BACTERIOL, V189, P844, DOI 10.1128/JB.01261-06; Korndorfer IP, 2004, NAT STRUCT MOL BIOL, V11, P1015, DOI 10.1038/nsmb828; Kozany C, 2004, NAT STRUCT MOL BIOL, V11, P234, DOI 10.1038/nsmb734; Kozjak-Pavlovic V, 2007, EMBO REP, V8, P576, DOI 10.1038/sj.embor.7400982; Kutik S, 2008, CELL, V132, P1011, DOI 10.1016/j.cell.2008.01.028; Kutik S, 2007, J CELL BIOL, V179, P585, DOI 10.1083/jcb.200708199; Kutik S, 2009, BBA-GEN SUBJECTS, V1790, P409, DOI 10.1016/j.bbagen.2009.04.004; Langer T, 2000, TRENDS BIOCHEM SCI, V25, P247, DOI 10.1016/S0968-0004(99)01541-8; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; Li ZW, 2006, NUCLEIC ACIDS RES, V34, pD277, DOI 10.1093/nar/gkj124; Lionaki E, 2008, J BIOL CHEM, V283, P15747, DOI 10.1074/jbc.M800350200; Lister R, 2007, PLANT CELL, V19, P3739, DOI 10.1105/tpc.107.050534; LITHGOW T, 1994, P NATL ACAD SCI USA, V91, P11973, DOI 10.1073/pnas.91.25.11973; Luo WT, 2006, MOL GENET GENOMICS, V275, P431, DOI 10.1007/s00438-006-0099-7; Macasev D, 2004, MOL BIOL EVOL, V21, P1557, DOI 10.1093/molbev/msh166; MacKenzie JA, 2007, BBA-MOL BASIS DIS, V1772, P509, DOI 10.1016/j.bbadis.2006.12.002; Marienfeld J, 1999, TRENDS PLANT SCI, V4, P495, DOI 10.1016/S1360-1385(99)01502-2; McQuibban GA, 2003, NATURE, V423, P537, DOI 10.1038/nature01633; Mesecke N, 2008, EMBO REP, V9, P1107, DOI 10.1038/embor.2008.173; Milenkovic D, 2004, J BIOL CHEM, V279, P22781, DOI 10.1074/jbc.C400120200; Milenkovic D, 2009, MOL BIOL CELL, V20, P2530, DOI 10.1091/mbc.E08-11-1108; Mirus O, 2009, J MOL MODEL, V15, P971, DOI 10.1007/s00894-008-0449-y; MOCZKO M, 1992, FEBS LETT, V310, P265, DOI 10.1016/0014-5793(92)81345-M; Mokranjac D, 2009, MOL BIOL CELL, V20, P1400, DOI 10.1091/mbc.E08-09-0934; Nakai K, 1999, TRENDS BIOCHEM SCI, V24, P34, DOI 10.1016/S0968-0004(98)01336-X; Nargang FE, 1998, MOL CELL BIOL, V18, P3173, DOI 10.1128/MCB.18.6.3173; Natale P, 2008, BBA-BIOMEMBRANES, V1778, P1735, DOI 10.1016/j.bbamem.2007.07.015; Neupert W, 2007, ANNU REV BIOCHEM, V76, P723, DOI 10.1146/annurev.biochem.76.052705.163409; Nolden M, 2005, CELL, V123, P277, DOI 10.1016/j.cell.2005.08.003; Otera H, 2005, EMBO J, V24, P1375, DOI 10.1038/sj.emboj.7600614; Paschen SA, 2005, TRENDS BIOCHEM SCI, V30, P575, DOI 10.1016/j.tibs.2005.08.009; Peeters N, 2001, BBA-MOL CELL RES, V1541, P54, DOI 10.1016/S0167-4889(01)00146-X; Peixoto PMV, 2007, J BIOL CHEM, V282, P18694, DOI 10.1074/jbc.M700775200; Pfanner N, 2004, NAT STRUCT MOL BIOL, V11, P1044, DOI 10.1038/nsmb852; Popov-Celeketic D, 2008, EMBO J, V27, P1469, DOI 10.1038/emboj.2008.79; PRATJE E, 1986, EMBO J, V5, P1313, DOI 10.1002/j.1460-2075.1986.tb04361.x; PRATJE E, 1983, EMBO J, V2, P1049, DOI 10.1002/j.1460-2075.1983.tb01544.x; Rassow J, 1999, J MOL BIOL, V286, P105, DOI 10.1006/jmbi.1998.2455; Sargent F, 1999, J BIOL CHEM, V274, P36073, DOI 10.1074/jbc.274.51.36073; Satow R, 2002, BIOCHEM BIOPH RES CO, V295, P85, DOI 10.1016/S0006-291X(02)00641-1; Senkevich TG, 2002, P NATL ACAD SCI USA, V99, P6667, DOI 10.1073/pnas.062163799; Settles AM, 1998, TRENDS CELL BIOL, V8, P494, DOI 10.1016/S0962-8924(98)01387-7; Sickmann A, 2003, P NATL ACAD SCI USA, V100, P13207, DOI 10.1073/pnas.2135385100; SIPPL MJ, 1993, J COMPUT AID MOL DES, V7, P473, DOI 10.1007/BF02337562; Small I, 2004, PROTEOMICS, V4, P1581, DOI 10.1002/pmic.200300776; Smid O, 2008, PLOS PATHOG, V4, DOI 10.1371/journal.ppat.1000243; Sonnhammer E L, 1998, Proc Int Conf Intell Syst Mol Biol, V6, P175; Souza RL, 2000, J BIOL CHEM, V275, P14898, DOI 10.1074/jbc.275.20.14898; STEGER HF, 1990, J CELL BIOL, V111, P2353, DOI 10.1083/jcb.111.6.2353; Stojanovski D, 2007, J CELL BIOL, V179, P881, DOI 10.1083/jcb.200706043; Stuart RA, 2002, BBA-MOL CELL RES, V1592, P79, DOI 10.1016/S0167-4889(02)00266-5; Szyrach G, 2003, EMBO J, V22, P6448, DOI 10.1093/emboj/cdg623; Tatusov RL, 2003, BMC BIOINFORMATICS, V4, DOI 10.1186/1471-2105-4-41; Thomas PD, 2003, GENOME RES, V13, P2129, DOI 10.1101/gr.772403; Thorpe C, 2002, ARCH BIOCHEM BIOPHYS, V405, P1, DOI 10.1016/S0003-9861(02)00337-5; TSAOUSIS AD, 2010, MOL BIOL EVOL; Urban S, 2001, CELL, V107, P173, DOI 10.1016/S0092-8674(01)00525-6; van der Merwe JA, 2007, J PLANT PHYSIOL, V164, P1231, DOI 10.1016/j.jplph.2006.11.009; van Lis R, 2003, PLANT PHYSIOL, V132, P318, DOI 10.1104/pp.102.018325; VANLOON APGM, 1988, J CELL BIOCHEM, V36, P59, DOI 10.1002/jcb.240360107; Wang XJ, 2007, MOL BIOL EVOL, V24, P363, DOI 10.1093/molbev/msl167; Webb CT, 2006, MOL CELL, V21, P123, DOI 10.1016/j.molcel.2005.11.010; Weber ER, 1996, MOL BIOL CELL, V7, P307; Whitworth AJ, 2008, DIS MODEL MECH, V1, P168, DOI 10.1242/dmm.000109; Wiederstein M, 2007, NUCLEIC ACIDS RES, V35, pW407, DOI 10.1093/nar/gkm290; Wu LF, 2000, J MOL MICROB BIOTECH, V2, P179; Yamano K, 2008, J BIOL CHEM, V283, P3799, DOI 10.1074/jbc.M708339200; Yano M, 2004, J BIOL CHEM, V279, P10808, DOI 10.1074/jbc.M311710200; Ye YZ, 2004, NUCLEIC ACIDS RES, V32, pW582, DOI 10.1093/nar/gkh430; Yu CS, 2006, PROTEINS, V64, P643, DOI 10.1002/prot.21018; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847; Zhang YJ, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-137	133	10	10	0	5	PUBLIC LIBRARY SCIENCE	SAN FRANCISCO	1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA	1932-6203			PLOS ONE	PLoS One	MAY 18	2011	6	5							e19540	10.1371/journal.pone.0019540			18	Multidisciplinary Sciences	Science & Technology - Other Topics	765PE	WOS:000290720200014	21611166	DOAJ Gold, Green Published			2021-04-07	
J	Tenhaken, R; Voglas, E; Cock, JM; Neu, V; Huber, CG				Tenhaken, Raimund; Voglas, Elena; Cock, J. Mark; Neu, Volker; Huber, Christian G.			Characterization of GDP-mannose Dehydrogenase from the Brown Alga Ectocarpus siliculosus Providing the Precursor for the Alginate Polymer	JOURNAL OF BIOLOGICAL CHEMISTRY			English	Article							UDP-GLUCOSE DEHYDROGENASE; PSEUDOMONAS-AERUGINOSA; KEY ENZYME; MATRIX POLYSACCHARIDES; BIOSYNTHESIS; PURIFICATION; EVOLUTION; RESIDUES; SEQUENCE; GENOME	Alginate is a major cell wall polymer of brown algae. The precursor for the polymer is GDP-mannuronic acid, which is believed to be derived from a four-electron oxidation of GDPmannose through the enzyme GDP-mannose dehydrogenase (GMD). So far no eukaryotic GMD has been biochemically characterized. We have identified a candidate gene in the Ectocarpus siliculosus genome and expressed it as a recombinant protein in Escherichia coli. The GMD from Ectocarpus differs strongly from related enzymes in bacteria and is as distant to the bacterial proteins as it is to the group of UDP-glucose dehydrogenases. It lacks the C-terminal similar to 120 amino acid domain present in bacterial GMDs, which is believed to be involved in catalysis. The GMD from brown algae is highly active at alkaline pH and contains a catalytic Cys residue, sensitive to heavy metals. The product GDP-mannuronic acid was analyzed by HPLC and mass spectroscopy. The K-m for GDP-mannose was 95 mu M, and 86 mu M for NAD(+). No substrate other than GDP-mannose was oxidized by the enzyme. In gel filtration experiments the enzyme behaved as a dimer. The Ectocarpus GMD is stimulated by salts even at low molar concentrations as a possible adaptation to marine life. It is rapidly inactivated at temperatures above 30 degrees C.	[Tenhaken, Raimund; Voglas, Elena] Salzburg Univ, Dept Cell Biol, Div Plant Physiol, A-5020 Salzburg, Austria; [Cock, J. Mark] Marine Plants & Biomol Stn, Algal Genet Grp, F-29682 Roscoff, France; [Neu, Volker; Huber, Christian G.] Salzburg Univ, Dept Mol Biol, Div Chem & Bioanalyt, A-5020 Salzburg, Austria	Tenhaken, R (corresponding author), Salzburg Univ, Dept Cell Biol, Div Plant Physiol, Hellbrunnerstr 34, A-5020 Salzburg, Austria.	raimund.tenhaken@sbg.ac.at	Tenhaken, Raimund/O-6556-2019; Huber, Christian G./E-5445-2011	Tenhaken, Raimund/0000-0002-6764-4826; Huber, Christian G./0000-0001-8358-1880; Cock, J. Mark/0000-0002-2650-0383			Campbell RE, 2000, BIOCHEMISTRY-US, V39, P7012, DOI 10.1021/bi000181h; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Conklin PL, 1999, P NATL ACAD SCI USA, V96, P4198, DOI 10.1073/pnas.96.7.4198; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; DERETIC V, 1987, J BACTERIOL, V169, P351, DOI 10.1128/jb.169.1.351-358.1987; Draget K. I., 2002, BIOPOLYMERS POLYSACC, VII, P215; Ge X, 2004, EUR J BIOCHEM, V271, P14, DOI 10.1046/j.1432-1033.2003.03876.x; Hinterberg B, 2002, PLANT PHYSIOL BIOCH, V40, P1011, DOI 10.1016/S0981-9428(02)01465-1; Klinghammer M, 2007, J EXP BOT, V58, P3609, DOI 10.1093/jxb/erm209; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; LIN TY, 1966, J BIOL CHEM, V241, P5284; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Naught LE, 2002, BIOCHEMISTRY-US, V41, P9637, DOI 10.1021/bi025862m; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; ROYCHOUDHURY S, 1989, J BIOL CHEM, V264, P9380; Sheffield P, 1999, PROTEIN EXPRES PURIF, V15, P34, DOI 10.1006/prep.1998.1003; SKJAKBRAEK G, 1986, CARBOHYD RES, V154, P239; Snook CF, 2003, BIOCHEMISTRY-US, V42, P4658, DOI 10.1021/bi027328k	19	19	19	0	15	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC	BETHESDA	9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA		1083-351X		J BIOL CHEM	J. Biol. Chem.	MAY 13	2011	286	19					16707	16715		10.1074/jbc.M111.230979			9	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	760CZ	WOS:000290301900018	21454608	Other Gold, Green Published			2021-04-07	
J	Le Bail, A; Billoud, B; Le Panse, S; Chenivesse, S; Charrier, B				Le Bail, Aude; Billoud, Bernard; Le Panse, Sophie; Chenivesse, Sabine; Charrier, Benedicte			ETOILE Regulates Developmental Patterning in the Filamentous Brown Alga Ectocarpus siliculosus	PLANT CELL			English	Article							PROBE LEVEL DATA; CELL-WALL; EXTRACELLULAR-MATRIX; NOTCH; EXPRESSION; PHAEOPHYCEAE; NORMALIZATION; EUKARYOTES; EVOLUTION; POLARITY	Brown algae are multicellular marine organisms evolutionarily distant from both metazoans and land plants. The molecular or cellular mechanisms that govern the developmental patterning in brown algae are poorly characterized. Here, we report the first morphogenetic mutant, etoile (etl), produced in the brown algal model Ectocarpus siliculosus. Genetic, cellular, and morphometric analyses showed that a single recessive locus, ETL, regulates cell differentiation: etl cells display thickening of the extracellular matrix (ECM), and the elongated, apical, and actively dividing E cells are underrepresented. As a result of this defect, the overrepresentation of round, branch-initiating R cells in the etl mutant leads to the rapid induction of the branching process at the expense of the uniaxial growth in the primary filament. Computational modeling allowed the simulation of the etl mutant phenotype by including a modified response to the neighborhood information in the division rules used to specify wild-type development. Microarray experiments supported the hypothesis of a defect in cell-cell communication, as primarily Lin-Notch-domain transmembrane proteins, which share similarities with metazoan Notch proteins involved in binary cell differentiation were repressed in etl. Thus, our study highlights the role of the ECM and of novel transmembrane proteins in cell-cell communication during the establishment of the developmental pattern in this brown alga.	[Le Bail, Aude; Billoud, Bernard; Chenivesse, Sabine; Charrier, Benedicte] CNRS, Biol Stn, Unite Mixte Rech Vegetaux Marins & Biomol 7139, F-29682 Roscoff, France; [Le Bail, Aude; Billoud, Bernard; Chenivesse, Sabine; Charrier, Benedicte] Univ Paris 06, Unite Mixte Rech Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France	Charrier, B (corresponding author), CNRS, Biol Stn, Unite Mixte Rech Vegetaux Marins & Biomol 7139, F-29682 Roscoff, France.	charrier@sb-roscoff.fr		Billoud, Bernard/0000-0002-5140-8087; Charrier, Benedicte/0000-0001-5721-1640	Brittany Regional CouncilRegion Bretagne [ACOMB 'ECTOMUT-1790']	We thank Simon Dittami for help in the preparation and data analyses of the microarray experiment and for English corrections. We also thank Jean Sourimant for technical assistance with the scanning electron microscope and Beatrice Satiat-Jeunemaitre for her help with the interpretation of the Golgi apparatus structure. We thank Carole Maisonneuve for subculturing the etl mutant. A. L. B. was a fellow of the French Ministry of Higher Education and Research. The creation of the mutant library was financially supported by the Brittany Regional Council (Contract ACOMB 'ECTOMUT-1790').	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; ARTAVANISTSAKONAS S, 1995, SCIENCE, V268, P225, DOI 10.1126/science.7716513; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Baumberger N, 2003, PLANT J, V35, P71, DOI 10.1046/j.1365-313X.2003.01784.x; Belanger KD, 2003, PLANTA, V217, P931, DOI 10.1007/s00425-003-1058-8; BERGER F, 1994, SCIENCE, V263, P1421, DOI 10.1126/science.263.5152.1421; Billoud B, 2008, FUNCT PLANT BIOL, V35, P1014, DOI 10.1071/FP08036; Bolos V, 2007, ENDOCR REV, V28, P339, DOI 10.1210/er.2006-0046; Bolstad BM, 2003, BIOINFORMATICS, V19, P185, DOI 10.1093/bioinformatics/19.2.185; Bouget FY, 1998, DEVELOPMENT, V125, P1999; Brodersen P, 2008, SCIENCE, V320, P1185, DOI 10.1126/science.1159151; Brownlee C, 2002, CURR OPIN PLANT BIOL, V5, P396, DOI 10.1016/S1369-5266(02)00286-8; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dotto GP, 2008, ONCOGENE, V27, P5115, DOI 10.1038/onc.2008.225; Fiuza UM, 2007, J ENDOCRINOL, V194, P459, DOI 10.1677/JOE-07-0242; Fortini ME, 2009, DEV CELL, V16, P633, DOI 10.1016/j.devcel.2009.03.010; Fowler JE, 2004, PLANTA, V219, P856, DOI 10.1007/s00425-004-1283-9; Friedl P, 2010, CURR OPIN CELL BIOL, V22, P557, DOI 10.1016/j.ceb.2010.08.024; Gazave E, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-249; Hable WE, 2005, CELL MOTIL CYTOSKEL, V61, P9, DOI 10.1002/cm.20059; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Heinzelmann M, 1998, INFECT IMMUN, V66, P5842, DOI 10.1128/IAI.66.12.5842-5847.1998; Humphrey TV, 2007, NEW PHYTOL, V176, P7, DOI 10.1111/j.1469-8137.2007.02192.x; Irizarry RA, 2003, BIOSTATISTICS, V4, P249, DOI 10.1093/biostatistics/4.2.249; Irizarry RA, 2003, NUCLEIC ACIDS RES, V31, DOI 10.1093/nar/gng015; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Koch U, 2007, CELL MOL LIFE SCI, V64, P2746, DOI 10.1007/s00018-007-7164-1; Kohorn BD, 2001, CURR OPIN CELL BIOL, V13, P529, DOI 10.1016/S0955-0674(00)00247-7; Kohorn BD, 2000, PLANT PHYSIOL, V124, P31, DOI 10.1104/pp.124.1.31; Kohorn BD, 2009, PLANT J, V60, P974, DOI 10.1111/j.1365-313X.2009.04016.x; KROPF DL, 1992, MICROBIOL REV, V56, P316, DOI 10.1128/MMBR.56.2.316-339.1992; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail A, 2010, PLANT PHYSIOL, V153, P128, DOI 10.1104/pp.109.149708; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Liu H, 2009, CIRC RES, V104, P466, DOI 10.1161/CIRCRESAHA.108.184846; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Nagasato C, 2009, J PHYCOL, V45, P404, DOI 10.1111/j.1529-8817.2009.00655.x; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Prince RN, 2010, J CELL SCI, V123, P2308, DOI 10.1242/jcs.058321; Prusinkiewicz Przemyslaw, 1996, ALGORITHMIC BEAUTY P; R Development Core Team, 2011, R LANG ENV STAT COMP; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Saeed AI, 2003, BIOTECHNIQUES, V34, P374, DOI 10.2144/03342mt01; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Steinwand BJ, 2010, PLANT PHYSIOL, V153, P479, DOI 10.1104/pp.110.155887; Tusher VG, 2001, P NATL ACAD SCI USA, V98, P5116, DOI 10.1073/pnas.091062498; Voinnet O, 2009, CELL, V136, P669, DOI 10.1016/j.cell.2009.01.046; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847; Zhang ZH, 2010, NUCLEIC ACIDS RES, V38, pD806, DOI 10.1093/nar/gkp818	53	28	28	0	5	AMER SOC PLANT BIOLOGISTS	ROCKVILLE	15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA	1040-4651	1532-298X		PLANT CELL	Plant Cell	APR	2011	23	4					1666	1678		10.1105/tpc.110.081919			13	Biochemistry & Molecular Biology; Plant Sciences; Cell Biology	Biochemistry & Molecular Biology; Plant Sciences; Cell Biology	769GO	WOS:000291000500037	21478443	Green Published, Bronze			2021-04-07	
J	Dittami, SM; Gravot, A; Renault, D; Goulitquer, S; Eggert, A; Bouchereau, A; Boyen, C; Tonon, T				Dittami, Simon M.; Gravot, Antoine; Renault, David; Goulitquer, Sophie; Eggert, Anja; Bouchereau, Alain; Boyen, Catherine; Tonon, Thierry			Integrative analysis of metabolite and transcript abundance during the short-term response to saline and oxidative stress in the brown alga Ectocarpus siliculosus	PLANT CELL AND ENVIRONMENT			English	Article						abiotic stress response; amino acid metabolism; gamma-aminobutyric acid (GABA); osmolytes; phaeophyceae; polyunsaturated fatty acids; proline; urea	GAMMA-AMINOBUTYRIC-ACID; ARABIDOPSIS-THALIANA; LAMINARIA-DIGITATA; AMINOALDEHYDE DEHYDROGENASE; POLYAMINE METABOLISM; OSMOTIC-STRESS; SALT STRESS; PLANTS; BIOSYNTHESIS; TREHALOSE	The model brown alga Ectocarpus siliculosus undergoes extensive transcriptomic changes in response to abiotic stress, many of them related to primary metabolism and particularly to amino acid biosynthesis and degradation. In this study we seek to improve our knowledge of the mechanisms underlying the stress tolerance of this alga, in particular with regard to compatible osmolytes, by examining the effects of these changes on metabolite concentrations. We performed extensive metabolic profiling (urea, amino acids, sugars, polyols, organic acids, fatty acids) of Ectocarpus samples subjected to short-term hyposaline, hypersaline and oxidative stress, and integrated the results with previously published transcriptomic data. The most pronounced changes in metabolite concentrations occurred under hypersaline stress: both mannitol and proline were accumulated, but their low final concentrations indicate that, in this stress condition, both compounds are not likely to significantly contribute to osmoregulation at the level of the entire cell. Urea and trehalose were not detected in any of our samples. We also observed a shift in fatty acid composition from n-3 to n-6 fatty acids under high salinities, and demonstrated the salt stress-induced accumulation of small amounts of gamma-aminobutyric acid (GABA). GABA could be synthesized in E. siliculosus through a salt stress-induced putrescine-degradation pathway.	[Dittami, Simon M.; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France; [Dittami, Simon M.; Boyen, Catherine; Tonon, Thierry] CNRS, Biol Stn, UMR Marine Plants & Biomol 7139, F-29680 Roscoff, France; [Gravot, Antoine; Bouchereau, Alain] Univ Rennes 1, UMR INRA 118, F-35042 Rennes, France; [Renault, David] Univ Rennes 1, CNRS, UMR 6553, Equipe Paysages Changements Climat Biodivers, F-35042 Rennes, France; [Goulitquer, Sophie] Univ Bretagne Occidentale, Fac Med, INSERM U613, Lab Biochim Epissage Canc Lipides & Apoptose, F-29285 Brest, France; [Eggert, Anja] Leibniz Inst Balt Sea Res Warnemunde Phys Oceanog, D-18119 Rostock, Germany	Tonon, T (corresponding author), Univ Paris 06, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France.	tonon@sb-roscoff.fr	Dittami, Simon/E-8354-2011; Tonon, Thierry/A-3214-2009	Dittami, Simon/0000-0001-7987-7523; Tonon, Thierry/0000-0002-1454-6018; RENAULT, David/0000-0003-3644-1759	Rennes MetropoleRegion Bretagne; Region BretagneRegion Bretagne; European communityEuropean Commission [MESTCT 2005-020737]	We would like to thank Hugues Renault and Francois Larher for helpful discussions, and acknowledge Rennes Metropole and the Region Bretagne for financial support for the acquisition of the UPLC equipment. SMD received funding from the European community's Sixth Framework Programme (contract no. MESTCT 2005-020737).	Aziz A, 1998, J PLANT PHYSIOL, V153, P754, DOI 10.1016/S0176-1617(98)80231-9; BEALE RN, 1961, J CLIN PATHOL, V14, P418, DOI 10.1136/jcp.14.4.418; Bouche N, 2004, TRENDS PLANT SCI, V9, P110, DOI 10.1016/j.tplants.2004.01.006; Bouchereau A, 1999, PLANT SCI, V140, P103, DOI 10.1016/S0168-9452(98)00218-0; Branco-Price C, 2008, PLANT J, V56, P743, DOI 10.1111/j.1365-313X.2008.03642.x; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2007, NEW PHYTOL, V176, P45, DOI 10.1111/j.1469-8137.2007.02152.x; Cramer GR, 2007, FUNCT INTEGR GENOMIC, V7, P111, DOI 10.1007/s10142-006-0039-y; DAVISON IR, 1985, PHYCOLOGIA, V24, P449, DOI 10.2216/i0031-8884-24-4-449.1; Davison IR, 1996, J PHYCOL, V32, P197, DOI 10.1111/j.0022-3646.1996.00197.x; Di Martino C, 2003, NEW PHYTOL, V158, P455, DOI 10.1046/j.1469-8137.2003.00770.x; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Fait A, 2008, TRENDS PLANT SCI, V13, P14, DOI 10.1016/j.tplants.2007.10.005; Foyer CH, 2003, J EXP BOT, V54, P585, DOI 10.1093/jxb/erg053; Gibon Y, 2004, PLANT CELL, V16, P3304, DOI 10.1105/tpc.104.025973; Gibon Y, 2006, GENOME BIOL, V7, DOI 10.1186/gb-2006-7-8-r76; Gong QQ, 2005, PLANT J, V44, P826, DOI 10.1111/j.1365-313X.2005.02587.x; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Jubault M, 2008, PLANT PHYSIOL, V146, P2008, DOI 10.1104/pp.108.117432; Kaplan F, 2004, PLANT PHYSIOL, V136, P4159, DOI 10.1104/pp.104.052142; Kaplan F, 2007, PLANT J, V50, P967, DOI 10.1111/j.1365-313X.2007.03100.x; KARSTEN U, 1991, PLANT PHYSIOL BIOCH, V29, P373; Kempa S, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0003935; Kinnersley AM, 2000, CRIT REV PLANT SCI, V19, P479, DOI 10.1016/S0735-2689(01)80006-X; Krell A, 2007, J PHYCOL, V43, P753, DOI 10.1111/j.1529-8817.2007.00366.x; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; KUMAR PP, 1989, PHYSIOL PLANTARUM, V76, P521, DOI 10.1111/j.1399-3054.1989.tb05472.x; Le Quere V, 2004, J LIPID RES, V45, P1446, DOI 10.1194/jlr.M300463-JLR200; Li Ke, 2008, Neurosci Bull, V24, P195, DOI 10.1007/s12264-008-0109-3; Lunn JE, 2006, BIOCHEM J, V397, P139, DOI 10.1042/BJ20060083; Malatrasi M, 2006, THEOR APPL GENET, V113, P965, DOI 10.1007/s00122-006-0339-6; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Mittova V, 2003, FEBS LETT, V554, P417, DOI 10.1016/S0014-5793(03)01214-6; Mostaert AS, 1995, PHYCOLOGIA, V34, P501, DOI 10.2216/i0031-8884-34-6-501.1; Muller J, 1995, PLANT SCI, V112, P1, DOI 10.1016/0168-9452(95)04218-J; Muller J, 2001, PLANT PHYSIOL, V125, P1086, DOI 10.1104/pp.125.2.1086; Noctor G, 1999, J EXP BOT, V50, P1157, DOI 10.1093/jexbot/50.336.1157; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Petrivalsky M, 2007, J PLANT PHYSIOL, V164, P1410, DOI 10.1016/j.jplph.2007.01.018; RASTOGI R, 1990, PLANT PHYSIOL, V94, P1449, DOI 10.1104/pp.94.3.1449; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; Renault H, 2010, BMC PLANT BIOL, V10, DOI 10.1186/1471-2229-10-20; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Roessner U, 2001, PLANT CELL, V13, P11, DOI 10.1105/tpc.13.1.11; ROUSVOAL S, 2011, MANNITOL 1 PHOSPHATE, DOI DOI 10.1007/S00425-010-1295-6; Sanina NM, 2008, PHYTOCHEMISTRY, V69, P1517, DOI 10.1016/j.phytochem.2008.01.014; Sebela M, 2000, BBA-PROTEIN STRUCT M, V1480, P329, DOI 10.1016/S0167-4838(00)00086-8; SMIRNOFF N, 1989, PHYTOCHEMISTRY, V28, P1057, DOI 10.1016/0031-9422(89)80182-7; Soderquist RG, 2005, PLANT CELL REP, V24, P127, DOI 10.1007/s00299-005-0918-z; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Takagi H, 2008, APPL MICROBIOL BIOT, V81, P211, DOI 10.1007/s00253-008-1698-5; Tausz M, 2004, J EXP BOT, V55, P1955, DOI 10.1093/jxb/erh194; TERANO S, 1978, PHYTOCHEMISTRY, V17, P550, DOI 10.1016/S0031-9422(00)89362-0; THOMAS DN, 1991, PHYSIOL PLANTARUM, V83, P281, DOI 10.1034/j.1399-3054.1991.830212.x; Zhang W, 2009, PLANT PHYSIOL, V149, P1773, DOI 10.1104/pp.108.133744	57	65	67	0	50	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0140-7791	1365-3040		PLANT CELL ENVIRON	Plant Cell Environ.	APR	2011	34	4					629	642		10.1111/j.1365-3040.2010.02268.x			14	Plant Sciences	Plant Sciences	732VV	WOS:000288218700008	21281312	Bronze			2021-04-07	
J	Rousvoal, S; Groisillier, A; Dittami, SM; Michel, G; Boyen, C; Tonon, T				Rousvoal, Sylvie; Groisillier, Agnes; Dittami, Simon M.; Michel, Gurvan; Boyen, Catherine; Tonon, Thierry			Mannitol-1-phosphate dehydrogenase activity in Ectocarpus siliculosus, a key role for mannitol synthesis in brown algae	PLANTA			English	Article						Brown algae; Ectocarpus; Enzymatic activity; Mannitol; Primary metabolism	PLATYMONAS-SUBCORDIFORMIS; OSMOTIC ROLE; RED; SEQUENCE; METABOLISM; PHOTOSYNTHESIS; PHAEOPHYTA; EVOLUTION; SALINITY; INSIGHTS	Mannitol represents a major end product of photosynthesis in brown algae (Phaeophyceae), and is, with the beta-1,3-glucan laminarin, the main form of carbon storage for these organisms. Despite its importance, little is known about the genes and enzymes responsible for the metabolism of mannitol in these seaweeds. Taking benefit of the sequencing of the Ectocarpus siliculosus genome, we focussed our attention on the first step of the synthesis of mannitol (reduction of the photo-assimilate fructose-6-phosphate), catalysed by the mannitol-1-phosphate dehydrogenase (M1PDH). This activity was measured in algal extracts, and was shown to be regulated by NaCl concentration in the reaction medium. Genomic analysis revealed the presence of three putative M1PDH genes (named EsM1PHD1, EsM1PDH2 and EsM1PDH3). Sequence comparison with orthologs demonstrates the modular architecture of EsM1PHD1 and EsM1PDH2, with an additional N-terminal domain of unknown function. In addition, gene expression experiments carried out on samples harvested through the diurnal cycle, and after several short-term saline and oxidative stress treatments, showed that EsM1PDH1 is the most highly expressed of these genes, whatever the conditions tested. In order to assess the activity of the corresponding protein, this gene was expressed in Escherichia coli. Cell-free extracts prepared from bacteria containing EsM1PDH1 displayed higher M1PDH activity than bacteria transformed with an empty plasmid. Further characterisation of recombinant EsM1PDH1 activity revealed its very narrow substrate specificity, salt regulation, and sensitivity towards an inhibitor of SH-enzymes.	[Tonon, Thierry] UPMC, CNRS, Biol Stn, UMR 7139, F-29682 Roscoff, France; [Rousvoal, Sylvie; Groisillier, Agnes; Dittami, Simon M.; Michel, Gurvan; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, UPMC, Biol Stn, UMR Marine Plants & Biomol 7139, F-29682 Roscoff, France; [Rousvoal, Sylvie; Groisillier, Agnes; Dittami, Simon M.; Michel, Gurvan; Boyen, Catherine; Tonon, Thierry] CNRS, Biol Stn, UMR Marine Plants & Biomol 7139, F-29682 Roscoff, France	Tonon, T (corresponding author), UPMC, CNRS, Biol Stn, UMR 7139, Pl Georges Teissier, F-29682 Roscoff, France.	tonon@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; Dittami, Simon/E-8354-2011; MICHEL, Gurvan/B-3490-2008	Tonon, Thierry/0000-0002-1454-6018; Dittami, Simon/0000-0001-7987-7523; MICHEL, Gurvan/0000-0002-3009-6205	European communityEuropean Commission [MESTCT 2005-020737]; NoE (Network of Excellence) European CommissionEuropean CommissionEuropean Commission Joint Research Centre [GOCE-CT-2004-505403]	SD received funding from the European community's Sixth Framework Program (contract n<SUP>o</SUP> MESTCT 2005-020737). Part of this work was performed within the framework of the 'Marine Genomics Europe' NoE (Network of Excellence) (European Commission contract No. GOCE-CT-2004-505403).	Akazaki H, 2008, ACTA CRYSTALLOGR F, V64, P674, DOI 10.1107/S1744309108017752; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Callebaut I, 1997, CELL MOL LIFE SCI, V53, P621, DOI 10.1007/s000180050082; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; DAVISON IR, 1985, PHYCOLOGIA, V24, P449, DOI 10.2216/i0031-8884-24-4-449.1; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Eggert A, 2007, PHYCOLOGIA, V46, P22, DOI 10.2216/06-12.1; Eggert A, 2006, EUR J PHYCOL, V41, P405, DOI 10.1080/09670260600919831; Gravot A, 2010, NEW PHYTOL, V188, P98, DOI 10.1111/j.1469-8137.2010.03400.x; Groisillier A, 2010, MICROB CELL FACT, V9, DOI 10.1186/1475-2859-9-45; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; HELLEBUST JA, 1976, CAN J BOT, V54, P1735, DOI 10.1139/b76-187; Herve C, 2008, BIOCHEM J, V412, P535, DOI 10.1042/BJ20071464; IKAWA T, 1972, PLANT CELL PHYSIOL, V13, P1017; Iwamoto K, 2005, MAR BIOTECHNOL, V7, P407, DOI 10.1007/s10126-005-0029-4; Iwamoto K, 2003, PLANT PHYSIOL, V133, P893, DOI 10.1104/pp.103.026906; Iwamoto K, 2001, MAR BIOTECHNOL, V3, P493, DOI 10.1007/s10126-001-0068-4; JIANG W, 1990, MOL MICROBIOL, V4, P2003, DOI 10.1111/j.1365-2958.1990.tb02050.x; KARSTEN U, 1992, J PLANT PHYSIOL, V140, P292, DOI 10.1016/S0176-1617(11)81081-3; KARSTEN U, 1993, AUST J PLANT PHYSIOL, V20, P729, DOI 10.1071/PP9930729; Karsten U, 1997, PLANTA, V201, P173, DOI 10.1007/BF01007701; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; KIRST GO, 1975, Z PFLANZENPHYSIOL, V76, P316, DOI 10.1016/S0044-328X(75)80058-4; KIRST GO, 1990, ANNU REV PLANT PHYS, V41, P21, DOI 10.1146/annurev.pp.41.060190.000321; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lesk AM, 1995, CURR OPIN STRUC BIOL, V5, P775, DOI 10.1016/0959-440X(95)80010-7; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Mostaert AS, 1995, PHYCOLOGIA, V34, P501, DOI 10.2216/i0031-8884-34-6-501.1; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; RICHTER DFE, 1987, PLANTA, V170, P528, DOI 10.1007/BF00402987; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Schmatz DM, 1997, PARASITOLOGY, V114, pS81; SCHNEIDER KH, 1993, J GEN MICROBIOL, V139, P2475, DOI 10.1099/00221287-139-10-2475; Solomon PS, 2007, TRENDS MICROBIOL, V15, P257, DOI 10.1016/j.tim.2007.04.002; Stoop JMH, 1996, TRENDS PLANT SCI, V1, P139, DOI 10.1016/S1360-1385(96)80048-3; Studier FW, 2005, PROTEIN EXPRES PURIF, V41, P207, DOI 10.1016/j.pep.2005.01.016; THOMAS DN, 1991, BOT ACTA, V104, P26, DOI 10.1111/j.1438-8677.1991.tb00190.x; THOMAS DN, 1991, PHYSIOL PLANTARUM, V83, P281, DOI 10.1034/j.1399-3054.1991.830212.x; Velez H, 2007, FUNGAL GENET BIOL, V44, P258, DOI 10.1016/j.fgb.2006.09.008; Wisselink HW, 2002, INT DAIRY J, V12, P151, DOI 10.1016/S0958-6946(01)00153-4; Wong TKM, 2007, J PHYCOL, V43, P528, DOI 10.1111/j.1529-8817.2007.00349.x; Worden AZ, 2009, SCIENCE, V324, P268, DOI 10.1126/science.1167222; WRIGHT PJ, 1989, J EXP BOT, V40, P1347, DOI 10.1093/jxb/40.12.1347; YAMAGUCH.T, 1969, PLANT CELL PHYSIOL, V10, P425; YAMAGUCHI T, 1966, PLANT CELL PHYSIOL, V7, P217; Zubia M, 2008, J APPL PHYCOL, V20, P1033, DOI 10.1007/s10811-007-9303-3	50	39	39	2	37	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0032-0935	1432-2048		PLANTA	Planta	FEB	2011	233	2					261	273		10.1007/s00425-010-1295-6			13	Plant Sciences	Plant Sciences	711TB	WOS:000286613200005	20981555				2021-04-07	
J	Dittami, SM; Proux, C; Rousvoal, S; Peters, AF; Cock, JM; Coppee, JY; Boyen, C; Tonon, T				Dittami, Simon M.; Proux, Caroline; Rousvoal, Sylvie; Peters, Akira F.; Cock, J. Mark; Coppee, Jean-Yves; Boyen, Catherine; Tonon, Thierry			Microarray estimation of genomic inter-strain variability in the genus Ectocarpus (Phaeophyceae)	BMC MOLECULAR BIOLOGY			English	Article							FUCOXANTHIN-CHLOROPHYLL PROTEINS; DENSITY OLIGONUCLEOTIDE ARRAYS; DNA; SILICULOSUS; SEQUENCE; STRESS; MODEL; EVOLUTION; EXPRESSION; DIVERSITY	Background: Brown algae of the genus Ectocarpus exhibit high levels of genetic diversity and variability in morphological and physiological characteristics. With the establishment of E. siliculosus as a model and the availability of a complete genome sequence, it is now of interest to analyze variability among different species, ecotypes, and strains of the genus Ectocarpus both at the genome and the transcriptome level. Results: We used an E. siliculosus gene expression microarray based on EST sequences from the genome-sequenced strain (reference strain) to carry out comparative genome hybridizations for five Ectocarpus strains: four E. siliculosus isolates (the male genome strain, a female strain used for outcrosses with the genome strain, a strain isolated from freshwater, and a highly copper-tolerant strain), as well as one strain of the sister species E. fasciculatus. Our results revealed significant genomic differences between ecotypes of the same species, and enable the selection of conserved probes for future microarray experiments with these strains. In the two closely related strains (a male and a female strain used for crosses), genomic differences were also detected, but concentrated in two smaller genomic regions, one of which corresponds to a viral insertion site. Conclusion: The high variability between strains supports the concept of E. siliculosus as a complex of cryptic species. Moreover, our data suggest that several parts of the Ectocarpus genome may have evolved at different rates: high variability was detected particularly in transposable elements and fucoxanthin chlorophyll a/c binding proteins.	[Dittami, Simon M.; Rousvoal, Sylvie; Cock, J. Mark; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, UPMC, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France; [Dittami, Simon M.; Rousvoal, Sylvie; Cock, J. Mark; Boyen, Catherine; Tonon, Thierry] CNRS, Biol Stn, UMR Marine Plants & Biomol 7139, F-29680 Roscoff, France; [Proux, Caroline; Coppee, Jean-Yves] Inst Pasteur, Plate Forme Puces ADN 2, F-75724 Paris 15, France; [Peters, Akira F.] MBA Lab, Plymouth PL1 2PB, Devon, England; [Peters, Akira F.] BEZHIN ROSKO, F-29250 Santec, France	Tonon, T (corresponding author), Univ Paris 06, UPMC, UMR Marine Plants & Biomol 7139, Biol Stn, F-29680 Roscoff, France.	tonon@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; Dittami, Simon/E-8354-2011	Tonon, Thierry/0000-0002-1454-6018; Dittami, Simon/0000-0001-7987-7523; Cock, J. Mark/0000-0002-2650-0383; Peters, Akira/0000-0001-5332-199X	European communityEuropean Commission [MESTCT 2005-020737]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [mba010003] Funding Source: researchfish	We would like to thank Declan Schroeder for helpful discussions, Aude Le Bail for providing material of strain 2, and Andres Ritter for providing material of strain 4. SD received funding from the European community's Sixth Framework Programme (contract n<SUP>o</SUP> MESTCT 2005-020737).	Amtmann A, 2009, MOL PLANT, V2, P3, DOI 10.1093/mp/ssn094; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Bar-Or C, 2007, TRENDS GENET, V23, P200, DOI 10.1016/j.tig.2007.02.003; Barrier M, 2003, GENETICS, V163, P723; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Beer A, 2006, BIOCHEMISTRY-US, V45, P13046, DOI 10.1021/bi061249h; BUARD J, 1994, EMBO J, V13, P3203, DOI 10.1002/j.1460-2075.1994.tb06619.x; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Dittami SM, 2010, BMC EVOL BIOL, V10, DOI 10.1186/1471-2148-10-365; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Gascuel O, 1997, MOL BIOL EVOL, V14, P685, DOI 10.1093/oxfordjournals.molbev.a025808; Gogvadze E, 2009, CELL MOL LIFE SCI, V66, P3727, DOI 10.1007/s00018-009-0107-2; Gonzalez J, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1000905; Gonzalez J, 2008, PLOS BIOL, V6, P2109, DOI 10.1371/journal.pbio.0060251; Gundermann K, 2008, PHOTOSYNTH RES, V95, P229, DOI 10.1007/s11120-007-9262-1; Hammond JP, 2005, PLANT METHODS, V1, DOI 10.1186/1746-4811-1-10; Hammond JP, 2006, NEW PHYTOL, V170, P239, DOI 10.1111/j.1469-8137.2006.01662.x; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Hiramatsu T, 2006, J PHYCOL, V42, P1048, DOI 10.1111/j.1529-8817.2006.00259.x; Hwang YS, 2008, BIOCHEM BIOPH RES CO, V367, P635, DOI 10.1016/j.bbrc.2007.12.176; Ji W, 2004, NUCLEIC ACIDS RES, V32, DOI 10.1093/nar/gnh084; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; LAMPPA GK, 1979, PLANT PHYSIOL, V64, P126, DOI 10.1104/pp.64.1.126; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Maumus F, 2009, BMC GENOMICS, V10, DOI 10.1186/1471-2164-10-624; McCauley LAR, 2007, PHYCOLOGIA, V46, P429, DOI 10.2216/05-08.1; Mezard C, 2006, BIOCHEM SOC T, V34, P531, DOI 10.1042/BST0340531; MIYAMURA S, 1986, PROTOPLASMA, V133, P66, DOI 10.1007/BF01293188; Muller DG, 2000, BOT MAR, V43, P157, DOI 10.1515/BOT.2000.016; Neilson JAD, 2010, PHOTOSYNTH RES, V106, P57, DOI 10.1007/s11120-010-9576-2; Olshen AB, 2004, BIOSTATISTICS, V5, P557, DOI 10.1093/biostatistics/kxh008; Peers G, 2009, NATURE, V462, P518, DOI 10.1038/nature08587; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters AF, 2010, NEW PHYTOL, V188, P30, DOI 10.1111/j.1469-8137.2010.03303.x; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; Ranz JM, 2003, SCIENCE, V300, P1742, DOI 10.1126/science.1085881; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; Sealfon RSG, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-443; Staaf J, 2007, BMC GENOMICS, V8, DOI 10.1186/1471-2164-8-382; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Taboada EN, 2005, BMC GENOMICS, V6, P1, DOI 10.1186/1471-2164-6-78; Talavera G, 2007, SYST BIOL, V56, P564, DOI 10.1080/10635150701472164; West John A., 1996, Muelleria, V9, P29; Yang SS, 2010, BMC PLANT BIOL, V10, DOI 10.1186/1471-2229-10-85; Zemke-White WL, 1999, J APPL PHYCOL, V11, P369, DOI 10.1023/A:1008197610793; Zhang Z, 2000, J COMPUT BIOL, V7, P203, DOI 10.1089/10665270050081478	54	18	19	0	11	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2199			BMC MOL BIOL	BMC Mol. Biol.	JAN 13	2011	12								2	10.1186/1471-2199-12-2			12	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	711UJ	WOS:000286616600001	21226968	Other Gold, Green Published			2021-04-07	
J	Dittami, SM; Michel, G; Collen, J; Boyen, C; Tonon, T				Dittami, Simon M.; Michel, Gurvan; Collen, Jonas; Boyen, Catherine; Tonon, Thierry			Chlorophyll-binding proteins revisited - a multigenic family of light-harvesting and stress proteins from a brown algal perspective	BMC EVOLUTIONARY BIOLOGY			English	Article							INDEPENDENT EVOLUTION; PHYSCOMITRELLA-PATENS; CHONDRUS-CRISPUS; GENE FAMILY; RED ALGA; SEQUENCE; GREEN; EXPRESSION; RESPONSES; COMPLEX	Background: Chlorophyll-binding proteins (CBPs) constitute a large family of proteins with diverse functions in both light-harvesting and photoprotection. The evolution of CBPs has been debated, especially with respect to the origin of the LI818 subfamily, members of which function in non-photochemical quenching and have been found in chlorophyll a/c containing algae and several organisms of the green lineage, but not in red algae so far. The recent publication of the Ectocarpus siliculosus genome represents an opportunity to expand on previous work carried out on the origin and function of CBPs. Results: The Ectocarpus genome codes for 53 CBPs falling into all major families except the exclusively green family of chlorophyll a/b binding proteins. Most stress-induced CBPs belong to the LI818 family. However, we highlight a few stress-induced CBPs from Phaeodactylum tricomutum and Chondrus crispus that belong to different sub-families and are promising targets for future functional studies. Three-dimensional modeling of two LI818 proteins revealed features common to all LI818 proteins that are likely to interfere with their capacity to bind chlorophyll b and lutein, but may enable binding of chlorophyll c and fucoxanthin. In the light of this finding, we examined the possibility that LI818 proteins may have originated in a chlorophyll c/fucoxanthin containing organism and compared this scenario to three alternatives: an independent evolution of LI818 proteins in different lineages, an ancient origin together with the first CBPs, before the separation of the red and the green lineage, or an origin in the green lineage and a transfer to an ancestor of haptophytes and heterokonts during a cryptic endosymbiosis event. Conclusions: Our findings reinforce the idea that the LI818 family of CBPs has a role in stress response. In addition, statistical analyses of phylogenetic trees show an independent origin in different eukaryotic lineages or a green algal origin of LI818 proteins to be highly unlikely. Instead, our data favor an origin in an ancestral chlorophyll a/c-containing organism and a subsequent lateral transfer to some green algae, although an origin of LI818 proteins in a common ancestor of red and green algae cannot be ruled out.	[Dittami, Simon M.; Michel, Gurvan; Collen, Jonas; Boyen, Catherine; Tonon, Thierry] UPMC Univ Paris 06, UMR Marine Plants & Biomol 7139, Stn Biol, F-29680 Roscoff, France; [Dittami, Simon M.; Michel, Gurvan; Collen, Jonas; Boyen, Catherine; Tonon, Thierry] CNRS, UMR Marine Plants & Biomol 7139, Stn Biol, F-29680 Roscoff, France	Dittami, SM (corresponding author), UPMC Univ Paris 06, UMR Marine Plants & Biomol 7139, Stn Biol, F-29680 Roscoff, France.	simon.dittami@bio.uio.no; tonon@sb-roscoff.fr	Dittami, Simon/E-8354-2011; Tonon, Thierry/A-3214-2009	Dittami, Simon/0000-0001-7987-7523; Tonon, Thierry/0000-0002-1454-6018; MICHEL, Gurvan/0000-0002-3009-6205	European communityEuropean Commission [MESTCT 2005-020737]	We would like to thank Odile Richard, Catherine Leblanc, Gildas Le Corguille, Shuhei Ota, Martin Lohr, and the reviewers of this paper for helpful discussions and/or comments. SD received funding from the European community's Sixth Framework Programme (contract no MESTCT 2005-020737).	Abascal F, 2005, BIOINFORMATICS, V21, P2104, DOI 10.1093/bioinformatics/bti263; Alboresi A, 2010, P NATL ACAD SCI USA, V107, P11128, DOI 10.1073/pnas.1002873107; Alboresi A, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002033; Archibald JM, 2009, CURR BIOL, V19, pR81, DOI 10.1016/j.cub.2008.11.067; Bailey B A, 2010, P APPR THEOR 13 SAN, P1; Baurain D, 2010, MOL BIOL EVOL, V27, P1698, DOI 10.1093/molbev/msq059; Beer A, 2006, BIOCHEMISTRY-US, V45, P13046, DOI 10.1021/bi061249h; Berney C, 2006, P ROY SOC B-BIOL SCI, V273, P1867, DOI 10.1098/rspb.2006.3537; Broughton MJ, 2006, GENE, V369, P72, DOI 10.1016/j.gene.2005.10.026; Burki F, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000790; Caron L, 2001, CAH BIOL MAR, V42, P109; Cavalier-Smith T, 1999, J EUKARYOT MICROBIOL, V46, P347, DOI 10.1111/j.1550-7408.1999.tb04614.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Collen J, 2006, J PHYCOL, V42, P104, DOI 10.1111/j.1529-8817.2006.00171.x; Collen J, 2007, NEW PHYTOL, V176, P45, DOI 10.1111/j.1469-8137.2007.02152.x; Cuming AC, 2007, NEW PHYTOL, V176, P275, DOI 10.1111/j.1469-8137.2007.02187.x; Dagan T, 2009, SCIENCE, V324, P1651, DOI 10.1126/science.1175765; DeLano WL, 2005, DRUG DISCOV TODAY, V10, P213, DOI 10.1016/S1359-6446(04)03363-X; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; DOUZERY EJP, 2004, PNAS, V101, P6; Elrad D, 2004, CURR GENET, V45, P61, DOI 10.1007/s00294-003-0460-x; Engelken J, 2010, BMC EVOL BIOL, V10, DOI 10.1186/1471-2148-10-233; Webb Benjamin, 2016, Curr Protoc Protein Sci, V86, DOI [10.1002/0471250953.bi0506s15, 10.1002/0471140864.ps0209s50, 10.1002/cpbi.3, 10.1002/cpps.20]; Frommolt R, 2008, MOL BIOL EVOL, V25, P2653, DOI 10.1093/molbev/msn206; Gouet P, 2003, NUCLEIC ACIDS RES, V31, P3320, DOI 10.1093/nar/gkg556; Gould SB, 2007, J BIOL CHEM, V282, P30295, DOI 10.1074/jbc.M701869200; GREEN BR, 1995, PHOTOSYNTH RES, V44, P139, DOI 10.1007/BF00018304; GREEN BR, 1991, TRENDS BIOCHEM SCI, V16, P181, DOI 10.1016/0968-0004(91)90072-4; GROSSMAN A, 1990, MOL GEN GENET, V224, P91, DOI 10.1007/BF00259455; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Gundermann K, 2008, PHOTOSYNTH RES, V95, P229, DOI 10.1007/s11120-007-9262-1; Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.5598/IMAFUNGUS.2011.02.02.06; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; Huelsenbeck JP, 2001, SCIENCE, V294, P2310, DOI 10.1126/science.1065889; Hwang YS, 2008, BIOCHEM BIOPH RES CO, V367, P635, DOI 10.1016/j.bbrc.2007.12.176; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Koziol AG, 2007, PLANT PHYSIOL, V143, P1802, DOI [10.1104/pp.106.092536, 10.1104/pp.092536]; KUHLBRANDT W, 1994, NATURE, V367, P614, DOI 10.1038/367614a0; LaRoche J, 1996, P NATL ACAD SCI USA, V93, P15244; LAROCHE J, 1994, PLANT MOL BIOL, V25, P355, DOI 10.1007/BF00043865; Lefebvre SC, 2010, J PHYCOL, V46, P123, DOI 10.1111/j.1529-8817.2009.00793.x; Liu ZF, 2004, NATURE, V428, P287, DOI 10.1038/nature02373; Matsuzaki M, 2004, NATURE, V428, P653, DOI 10.1038/nature02398; MAXWELL DP, 1995, PLANT PHYSIOL, V109, P787, DOI 10.1104/pp.109.3.787; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Miura K, 2004, PLANT PHYSIOL, V135, P1595, DOI 10.1104/pp.104.041400; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; Neilson JAD, 2010, PHOTOSYNTH RES, V106, P57, DOI 10.1007/s11120-010-9576-2; Nikaido I, 2000, DNA RES, V7, P223, DOI 10.1093/dnares/7.3.223; Nymark M, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0007743; Park S, 2010, PLANTA, V231, P349, DOI 10.1007/s00425-009-1044-x; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; Peers G, 2009, NATURE, V462, P518, DOI 10.1038/nature08587; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Reyes-Prieto A, 2006, CURR BIOL, V16, P2320, DOI 10.1016/j.cub.2006.09.063; Richard C, 2000, PLANT MOL BIOL, V42, P303, DOI 10.1023/A:1006340308077; Rogers MB, 2007, MOL BIOL EVOL, V24, P54, DOI 10.1093/molbev/msl129; Sanchez-Puerta MV, 2008, J PHYCOL, V44, P1097, DOI 10.1111/j.1529-8817.2008.00559.x; Savard F, 1996, PLANT MOL BIOL, V32, P461, DOI 10.1007/BF00019098; Schmidt HA, 2002, BIOINFORMATICS, V18, P502, DOI 10.1093/bioinformatics/18.3.502; Shimodaira H, 2001, BIOINFORMATICS, V17, P1246, DOI 10.1093/bioinformatics/17.12.1246; Shimodaira H, 2002, SYST BIOL, V51, P492, DOI 10.1080/10635150290069913; Stiller JW, 2009, BMC GENOMICS, V10, DOI 10.1186/1471-2164-10-484; Talavera G, 2007, SYST BIOL, V56, P564, DOI 10.1080/10635150701472164; Teramoto H, 2002, PLANT PHYSIOL, V130, P325, DOI 10.1104/pp.004622; TONKYN JC, 1992, PLANT PHYSIOL, V99, P1406, DOI 10.1104/pp.99.4.1406; Wolf L, 2010, PLANT PHYSIOL, V153, P1123, DOI 10.1104/pp.110.154658; Worden AZ, 2009, SCIENCE, V324, P268, DOI 10.1126/science.1167222; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847; Zhu S.H., 2008, PHOTOSYNTHESIS ENERG, P261	71	69	70	0	21	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2148			BMC EVOL BIOL	BMC Evol. Biol.	NOV 26	2010	10								365	10.1186/1471-2148-10-365			14	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	724KN	WOS:000287575100002	21110855	DOAJ Gold, Green Published			2021-04-07	
J	Carrie, C; Murcha, MW; Whelan, J				Carrie, Chris; Murcha, Monika W.; Whelan, James			An in silico analysis of the mitochondrial protein import apparatus of plants	BMC PLANT BIOLOGY			English	Article							CYTOCHROME-C REDUCTASE; PREPROTEIN TRANSLOCASE; PROCESSING PEPTIDASE; INTERMEMBRANE SPACE; MEMBRANE-PROTEINS; ALTERNATIVE OXIDASE; TARGETING PEPTIDES; GENOME SEQUENCE; GLOBAL ANALYSIS; INNER MEMBRANE	Background: An in silico analysis of the mitochondrial protein import apparatus from a variety of species; including Chlamydomonas reinhardtii, Chlorella variabilis, Ectocarpus siliculosus, Cyanidioschyzon merolae, Physcomitrella patens, Selaginella moellendorffii, Picea glauca, Oryza sativa and Arabidopsis thaliana was undertaken to determine if components differed within and between plant and non-plant species. Results: The channel forming subunits of the outer membrane components Tom40 and Sam50 are conserved between plant groups and other eukaryotes. In contrast, the receptor component(s) in green plants, particularly Tom20, (C. reinhardtii, C. variabilis, P. patens, S. moellendorffii, P. glauca, O. sativa and A. thaliana) are specific to this lineage. Red algae contain a Tom22 receptor that is orthologous to yeast Tom22. Furthermore, plant mitochondrial receptors display differences between various plant lineages. These are evidenced by distinctive motifs in all plant Metaxins, which are absent in red algae, and the presence of the outer membrane receptor OM64 in Angiosperms (rice and Arabidopsis), but not in lycophytes (S. moellendorffii) and gymnosperms (P. glauca). Furthermore, although the intermembrane space receptor Mia40 is conserved across a wide phylogenetic range, its function differs between lineages. In all plant lineages, Tim17 contains a C-terminal extension, which may act as a receptor component for the import of nucleic acids into plant mitochondria. Conclusions: It is proposed that the observed functional divergences are due to the selective pressure to sort proteins between mitochondria and chloroplasts, resulting in differences in protein receptor components between plant groups and other organisms. Additionally, diversity of receptor components is observed within the plant kingdom. Even when receptor components are orthologous across plant and non-plant species, it appears that the functions of these have expanded or diverged in a lineage specific manner.	[Carrie, Chris; Murcha, Monika W.; Whelan, James] Univ Western Australia, Australian Res Council Ctr Excellence Plant Energ, Crawley, WA 6009, Australia	Whelan, J (corresponding author), Univ Western Australia, Australian Res Council Ctr Excellence Plant Energ, 35 Stirling, Crawley, WA 6009, Australia.	seamus@cyllene.uwa.edu.au	Carrie, Chris/A-2638-2010; Carrie, Chris/Y-3336-2019; Murcha, Monika/H-5495-2014; Murcha, Monika/C-3834-2011; Whelan, James/F-6402-2011	Carrie, Chris/0000-0002-4240-4674; Carrie, Chris/0000-0002-4240-4674; Murcha, Monika/0000-0002-3689-6158; Whelan, James/0000-0001-5754-025X	Australian Research Council Centre of ExcellenceAustralian Research Council [CEO561495]	This work was supported by an Australian Research Council Centre of Excellence Grant CEO561495.	Adams KL, 2003, MOL PHYLOGENET EVOL, V29, P380, DOI 10.1016/S1055-7903(03)00194-5; Allen JWA, 2008, FEBS LETT, V582, P2817, DOI 10.1016/j.febslet.2008.07.015; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; ALVERSON AJ, 2010, MOL BIOL EVOL; [Anonymous], 2005, NATURE, V436, P793; Arabidopsis Genome Initiative, 2000, NATURE, V408, P796; Bailey T L, 1994, Proc Int Conf Intell Syst Mol Biol, V2, P28; Banks JA, 2009, ANNU REV PLANT BIOL, V60, P223, DOI 10.1146/annurev.arplant.59.032607.092851; Bhushan S, 2005, PLANT CELL PHYSIOL, V46, P985, DOI 10.1093/pcp/pci107; Bhushan S, 2003, EMBO REP, V4, P1073, DOI 10.1038/sj.embor.7400011; BLANC G, PLANT CELL; Bolte K, 2009, J EUKARYOT MICROBIOL, V56, P9, DOI 10.1111/j.1550-7408.2008.00370.x; Bonen L, 2008, MITOCHONDRION, V8, P26, DOI 10.1016/j.mito.2007.09.005; BRAUN HP, 1992, EMBO J, V11, P3219, DOI 10.1002/j.1460-2075.1992.tb05399.x; Brumme S, 1998, J BIOL CHEM, V273, P13143, DOI 10.1074/jbc.273.21.13143; CARRIE C, 2010, CHARACTERISATION DIS; Cavalier-Smith T, 2009, INT J BIOCHEM CELL B, V41, P307, DOI 10.1016/j.biocel.2008.10.002; Chacinska A, 2009, CELL, V138, P628, DOI 10.1016/j.cell.2009.08.005; Chan NC, 2006, J MOL BIOL, V358, P1010, DOI 10.1016/j.jmb.2006.02.062; Chew O, 2004, TRENDS PLANT SCI, V9, P318, DOI 10.1016/j.tplants.2004.05.003; Chew O, 2004, FEBS LETT, V557, P109, DOI 10.1016/S0014-5793(03)01457-1; Clements A, 2009, P NATL ACAD SCI USA, V106, P15791, DOI 10.1073/pnas.0908264106; COCK JM, NATURE, V465, P617; Considine MJ, 2002, PLANT PHYSIOL, V129, P949, DOI 10.1104/pp.004150; Cserzo M, 1997, PROTEIN ENG, V10, P673, DOI 10.1093/protein/10.6.673; DAY DA, 1995, AUST J PLANT PHYSIOL, V22, P497, DOI 10.1071/PP9950497; Deusch O, 2008, MOL BIOL EVOL, V25, P748, DOI 10.1093/molbev/msn022; Dimmer KS, 2008, GENOME BIOL, V9, DOI 10.1186/gb-2008-9-2-209; Dolezal P, 2006, SCIENCE, V313, P314, DOI 10.1126/science.1127895; Dolezal P, 2010, PLOS PATHOG, V6, DOI 10.1371/journal.ppat.1000812; Dyall SD, 2004, SCIENCE, V304, P253, DOI 10.1126/science.1094884; ELIYAHU E, MOL CELL BIOL, V30, P284; Endo T, 2010, ANTIOXID REDOX SIGN, V13, P1359, DOI 10.1089/ars.2010.3099; Fredslund J, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-315; Gentle IE, 2007, MOL BIOL EVOL, V24, P1149, DOI 10.1093/molbev/msm031; Gentle IE, 2005, MOL MICROBIOL, V58, P1216, DOI 10.1111/j.1365-2958.2005.04906.x; Glaser E, 1999, J BIOENERG BIOMEMBR, V31, P259, DOI 10.1023/A:1005475930477; Gould SB, 2008, ANNU REV PLANT BIOL, V59, P491, DOI 10.1146/annurev.arplant.59.032607.092915; Huang CY, 2003, NATURE, V422, P72, DOI 10.1038/nature01435; Huang SB, 2009, PLANT PHYSIOL, V150, P1272, DOI 10.1104/pp.109.137885; Jansch L, 1998, J BIOL CHEM, V273, P17251, DOI 10.1074/jbc.273.27.17251; Kambacheld M, 2005, J BIOL CHEM, V280, P20132, DOI 10.1074/jbc.M500398200; Karpenahalli MR, 2007, BMC BIOINFORMATICS, V8, DOI 10.1186/1471-2105-8-2; Katoh K, 2005, NUCLEIC ACIDS RES, V33, P511, DOI 10.1093/nar/gki198; Keeling PJ, 2010, PHILOS T R SOC B, V365, P729, DOI 10.1098/rstb.2009.0103; Kmiec-Wisniewska B, 2008, PLANT MOL BIOL, V68, P159, DOI 10.1007/s11103-008-9359-8; Kozjak-Pavlovic V, 2007, EMBO REP, V8, P576, DOI 10.1038/sj.embor.7400982; Likic VA, 2005, J MOL BIOL, V347, P81, DOI 10.1016/j.jmb.2004.12.057; Likic VA, 2010, METHODS MOL BIOL, V619, P271, DOI 10.1007/978-1-60327-412-8_16; Lister R, 2006, CURR BIOL, V16, pR197, DOI 10.1016/j.cub.2006.02.024; Lister R, 2003, NUCLEIC ACIDS RES, V31, P325, DOI 10.1093/nar/gkg055; Lister R, 2002, PLANT J, V30, P555, DOI 10.1046/j.1365-313X.2002.01316.x; Lister R, 2007, PLANT CELL, V19, P3739, DOI 10.1105/tpc.107.050534; Lithgow T, 2010, PHILOS T R SOC B, V365, P799, DOI 10.1098/rstb.2009.0167; Macasev D, 2004, MOL BIOL EVOL, V21, P1557, DOI 10.1093/molbev/msh166; Martin W, 1998, PLANT PHYSIOL, V118, P9, DOI 10.1104/pp.118.1.9; Martinez-Caballero S, 2007, J BIOL CHEM, V282, P3584, DOI 10.1074/jbc.M607551200; Matsuzaki M, 2004, NATURE, V428, P653, DOI 10.1038/nature02398; Meier S, 2005, J BIOL CHEM, V280, P7777, DOI 10.1074/jbc.M412158200; Mentel M, 2008, PHILOS T R SOC B, V363, P2717, DOI 10.1098/rstb.2008.0031; Merchant SS, 2007, SCIENCE, V318, P245, DOI 10.1126/science.1143609; MICHAUD M, PLANT MOL BIOL; Murcha MW, 2007, PLANT PHYSIOL, V143, P199, DOI 10.1104/pp.106.090688; Murcha MW, 2005, J BIOL CHEM, V280, P16476, DOI 10.1074/jbc.M413299200; Neupert W, 2007, ANNU REV BIOCHEM, V76, P723, DOI 10.1146/annurev.biochem.76.052705.163409; PALMER JD, 1988, J MOL EVOL, V28, P87, DOI 10.1007/BF02143500; Paschen SA, 2003, NATURE, V426, P862, DOI 10.1038/nature02208; Patron NJ, 2007, BIOESSAYS, V29, P1048, DOI 10.1002/bies.20638; Pavy N, 2005, BMC GENOMICS, V6, DOI 10.1186/1471-2164-6-144; Peixoto PMV, 2007, J BIOL CHEM, V282, P18694, DOI 10.1074/jbc.M700775200; Perry AJ, 2006, CURR BIOL, V16, P221, DOI 10.1016/j.cub.2005.12.034; Rassow J, 1999, J MOL BIOL, V286, P105, DOI 10.1006/jmbi.1998.2455; Rensing SA, 2008, SCIENCE, V319, P64, DOI 10.1126/science.1150646; Richardson AO, 2007, J EXP BOT, V58, P1, DOI 10.1093/jxb/erl148; RIEMER J, 2010, BIOCHIM BIOPHYS ACTA; Schmitz-Linneweber C, 2008, TRENDS PLANT SCI, V13, P663, DOI 10.1016/j.tplants.2008.10.001; Schneider A, 2008, TRENDS CELL BIOL, V18, P12, DOI 10.1016/j.tcb.2007.09.009; Schneider G, 1998, PROTEINS, V30, P49, DOI 10.1002/(SICI)1097-0134(19980101)30:1<49::AID-PROT5>3.3.CO;2-P; Soll J, 2004, NAT REV MOL CELL BIO, V5, P198, DOI 10.1038/nrm1333; Somerville C., 2002, BIOCH MOL BIOL PLANT, P456; Theissen U, 2006, CURR BIOL, V16, pR1016, DOI 10.1016/j.cub.2006.11.020; Truscott KN, 2001, NAT STRUCT BIOL, V8, P1074, DOI 10.1038/nsb726; van Wilpe S, 1999, NATURE, V401, P485, DOI 10.1038/46802; Vinh LS, 2004, MOL BIOL EVOL, V21, P1565, DOI 10.1093/molbev/msh176; Vogtle FN, 2009, CELL, V139, P428, DOI 10.1016/j.cell.2009.07.045; Waller RF, 2009, EUKARYOT CELL, V8, P19, DOI 10.1128/EC.00313-08; Werhahn W, 2001, PLANT PHYSIOL, V125, P943, DOI 10.1104/pp.125.2.943; Whelan S, 2001, MOL BIOL EVOL, V18, P691, DOI 10.1093/oxfordjournals.molbev.a003851; WOLFE KH, 1987, P NATL ACAD SCI USA, V84, P9054, DOI 10.1073/pnas.84.24.9054; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zhang XP, 2002, TRENDS PLANT SCI, V7, P14, DOI 10.1016/S1360-1385(01)02180-X	91	40	41	0	17	BIOMED CENTRAL LTD	LONDON	236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND	1471-2229			BMC PLANT BIOL	BMC Plant Biol.	NOV 16	2010	10								249	10.1186/1471-2229-10-249			15	Plant Sciences	Plant Sciences	686KV	WOS:000284696100001	21078193	DOAJ Gold, Green Published			2021-04-07	
J	Peters, AF; van Wijk, SJ; Cho, GY; Scornet, D; Hanyuda, T; Kawai, H; Schroeder, DC; Cock, JM; Boo, SM				Peters, Akira F.; van Wijk, Serinde J.; Cho, Ga Youn; Scornet, Delphine; Hanyuda, Takeaki; Kawai, Hiroshi; Schroeder, Declan C.; Cock, J. Mark; Boo, Sung Min			Reinstatement of Ectocarpus crouaniorum Thuret in Le Jolis as a third common species of Ectocarpus (Ectocarpales, Phaeophyceae) in Western Europe, and its phenology at Roscoff, Brittany	PHYCOLOGICAL RESEARCH			English	Article						biological species; Ectocarpus; genotyping; hybrid; internal transcribed spacer 1 length; phenology; phylogeny; post-zygotic sterility; taxonomy	BROWN-ALGAE; LIFE-CYCLE; SILICULOSUS PHAEOPHYCEAE; GENUS ECTOCARPUS; MODEL; INHERITANCE; SEXUALITY; GENETICS; BRITAIN	Based on morphological characters, cross-fertility and molecular systematics, two species are currently recognized in the ubiquitous temperate brown algal genus Ectocarpus: the type species E. siliculosus (Dillwyn) Lyngbye and E. fasciculatus Harvey. We studied diversity, cross-fertility and ecology of Ectocarpus in megatidal areas in northwest France (Western Europe) and propose to reinstate a third species, E. crouaniorum Thuret in Le Jolis. Genotyping of 67 individuals from five localities, including the type locality of E. crouaniorum, using internal transcribed spacer 1 (ITS1) length as a marker, showed that the three species co-occurred whenever the habitat was suitable. Our survey also revealed a single putative field hybrid between E. crouaniorum and E. siliculosus, and a single individual of a further Ectocarpus genotype. In laboratory experiments, E. crouaniorum was crossed with E. siliculosus and E. fasciculatus. In 12 of 13 crosses, the zygotes did not develop (postzygotic sterility); in one experiment a viable hybrid was produced after crossing a female E. crouaniorum with a male E. siliculosus, but this hybrid was unable to form meiospores. Phylogenetic analysis of five molecular markers from the nuclear, mitochondrial and plastid genomes (in total 1818 bp) confirmed genetic separation of the three species. Ecologically, E. crouaniorum was confined to high intertidal pools and run-offs, where the gametophyte was common from spring to summer. Another characteristic was that it usually occurred as an epiphyte of up to 12 cm in length on erect thalli of Scytosiphon lomentaria. Sporophytes of E. crouaniorum were found all year long; they were < 3 cm in size or microscopic and were epilithic in the same habitat. The presence of a third species of Ectocarpus in Western Europe suggests that species diversity in this genus is larger than recognized during the last 40 years.	[Peters, Akira F.; Scornet, Delphine; Cock, J. Mark] Bezhin Rosko, F-29680 Roscoff, France; [Peters, Akira F.; van Wijk, Serinde J.] CNRS, Biol Stn, UMR7139, Roscoff, France; [Peters, Akira F.; van Wijk, Serinde J.] Univ Paris 06, Roscoff, France; [Peters, Akira F.; Schroeder, Declan C.] Marine Biol Assoc UK, Plymouth, Devon, England; [Cho, Ga Youn; Boo, Sung Min] Chungnam Natl Univ, Dept Biol, Taejon, South Korea; [Hanyuda, Takeaki; Kawai, Hiroshi] Kobe Univ, Res Ctr Inland Seas, Kobe, Hyogo 657, Japan	Peters, AF (corresponding author), Bezhin Rosko, 28 Route Perharidy, F-29680 Roscoff, France.	akirapeters@gmail.com	Schroeder, Declan C./O-9131-2019	Schroeder, Declan C./0000-0001-5991-2838; Cock, J. Mark/0000-0002-2650-0383	French-Korean scientific cooperation [11836NH]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [MBA010001] Funding Source: researchfish	We thank Dieter G. Muller for providing strains of Ectocarpus and Kuckuckia and sharing unpublished data, and Willem Prud'homme van Reine for advice concerning the Ectocarpus taxa described by Kutzing. Collections at sites 6 and 7 were undertaken in the frame of French-Korean scientific cooperation (PAI STAR 2005/2006 project 11836NH to AFP and SMB). The study was started when the first author was working at the Station Biologique de Roscoff, and it was finished in the laboratory of the Marine Biological Association of the United Kingdom, Plymouth, thanks to a Ray Lankester Investigatorship for AFP.	ALTSHUL SF, 1997, NUCLEIC ACIDS RES, V25, P402; Batters EAL, 1902, J BOT LONDON       S, V40, P1; Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Coyer JA, 2002, P ROY SOC B-BIOL SCI, V269, P1829, DOI 10.1098/rspb.2002.2093; CROUAN PL, 1852, ALGUES MARINES FINIS; GALLARDO T, 1992, TAXON, V41, P324, DOI 10.2307/1222340; GUIRY M. D., 2009, ALGAEBASE; HAMEL G, 1939, BOT NOTISER, P65; Hamel G., 1931, PHEOPHYCEES FRANCE; HOEK C. VAN DEN, 1975, PHYCOLOGIA, V14, P317, DOI DOI 10.2216/I0031-8884-14-4-317.1; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; Kim H.S., 1992, KOREAN J PHYCOLOGY, V7, P225; KORNMANN P., 1956, PUBBL STAZ ZOOL NAPOLI, V28, P32; KORNMANN P, 1977, HELGOLAND WISS MEER, V29, P1, DOI 10.1007/BF01611137; KUCKUCK P, 1912, WISS MEERESUNTERS AB, V5, P155; Kuckuck P., 1958, HELGOLANDER WISS MEE, V6, P171; Kutzing F.T., 1860, TABULAE PHYCOLOGICAE, V10; Kutzing F.T., 1849, SPECIES ALGARUM; Kutzing F.T., 1843, PHYCOLOGIA GENERALIS, P1; KUTZING FT, 1855, TABULAE PHYCOLOGICAE, V5; KUTZING FT, 1845, PHYCOLOGIA GERMANICA; Le Corguille G, 2009, BMC EVOL BIOL, V9, DOI 10.1186/1471-2148-9-253; LeJolis A., 1863, LISTE ALGUES MARINES; Muller D. G., 1991, JPN J PHYCOL, V39, P151; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1976, J PHYCOL, V12, P252, DOI 10.1111/j.0022-3646.1976.00252.x; MULLER DG, 1966, PLANTA, V68, P57, DOI 10.1007/BF00385371; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1976, ARCH MICROBIOL, V109, P89, DOI 10.1007/BF00425117; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1994, EUR J PHYCOL, V29, P219, DOI 10.1080/09670269400650671; MULLER DG, 1977, BRIT PHYCOL J, V12, P131; MULLER DG, 1972, PHYCOLOGIA, V11, P11; Muller DG, 1997, PHYCOLOGIA, V36, P79, DOI 10.2216/i0031-8884-36-1-79.1; Ni-Ni-Win, 2008, PHYCOL RES, V56, P288, DOI 10.1111/j.1440-1835.2008.00510.x; Papenfuss GF, 1934, BOT GAZ, V96, P421; PEDERSEN PM, 1989, NORD J BOT, V9, P443, DOI 10.1111/j.1756-1051.1989.tb01024.x; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; Ricker RW, 1987, TAXONOMY BIOGEOGRAPH; RUSSELL G, 1967, J MAR BIOL ASSOC UK, V47, P233, DOI 10.1017/S0025315400033695; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; Santelices B., 1989, ALGAS MARINAS CHILE; SAUVAGEAU C, 1896, CR HEBD ACAD SCI, V123, P431; STACHE B, 1989, EVOLUTIONARY BIOGEOG, P173; STACHE B, 1993, KREUZUNGSEXPERIMENTE; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stegenga H., 1997, SEAWEEDS S AFRICAN W; Swofford DL, 2002, PAUP PHYLOGENETIC AN; VANREINE WFP, 1973, TAXON, V22, P93; White T.J., 1990, PCR PROTOCOLS GUIDE, P315	62	32	32	0	14	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	1322-0829	1440-1835		PHYCOL RES	Phycol. Res.	JUL	2010	58	3					157	170		10.1111/j.1440-1835.2010.00574.x			14	Marine & Freshwater Biology	Marine & Freshwater Biology	615XI	WOS:000279171500001					2021-04-07	
J	Rui, F; Boland, W				Rui, Fabio; Boland, Wilhelm			Algal Pheromone Biosynthesis: Stereochemical Analysis and Mechanistic Implications in Gametes of Ectocarpus siliculosus	JOURNAL OF ORGANIC CHEMISTRY			English	Article							MARINE BROWN-ALGAE; PERICYCLIC-REACTIONS; COPE REARRANGEMENT; ACID; OXYLIPINS; CATALYSIS; OXIDATION; EPOXIDES; ALCOHOLS; DEFENSE	During sexual reproduction, female gametes or eggs of brown algae release pheromones to attract their male mating partners. The biologically active compounds comprise linear or alicyclic unsaturated hydrocarbons derived from the aliphatic terminus of C-20 polyunsaturated fatty acids (PUFAs) by oxidative cleavage. The current study addresses the stereochemical course of the pheromone biosynthesis using female gametes of the marine brown alga E. siliculosus and chiral deuterium-labeled arachidonic acids. The biosynthetic sequence is likely to proceed via an intermediary 9-hydroperoxyarachidonic acid, which is cleaved with loss of the C(16)-H-R into the C-II-hydrocarbon dictyopterene C and 9-oxonona-(5Z,7E)-dienoic acid.	[Rui, Fabio; Boland, Wilhelm] Max Planck Inst Chem Ecol, Dept Bioorgan Chem, D-07745 Jena, Germany	Boland, W (corresponding author), Max Planck Inst Chem Ecol, Dept Bioorgan Chem, Hans Knoll Str 8, D-07745 Jena, Germany.	boland@ice.mpg.de	Boland, Wilhelm/K-7762-2012; Lenka, Sangram K./A-5830-2009	Boland, Wilhelm/0000-0001-6784-2534; Lenka, Sangram K./0000-0002-0121-2430	Max Planck SocietyMax Planck SocietyFoundation CELLEX	We thank Dr. Chuanghai Xia for kindly providing the intermediate 12, Prof. D. G. Muller for the gift of the strain of E. siliculosus used for the investigations, and Daniela Schmid for support M algal cultivation techniques and fruitful discussions. The Max Planck Society is acknowledged for funding.	Abad JL, 2000, J ORG CHEM, V65, P8582, DOI 10.1021/jo000957k; Abad JL, 2007, J ORG CHEM, V72, P760, DOI 10.1021/jo061592s; BOLAND W, 1984, EUR J BIOCHEM, V144, P169, DOI 10.1111/j.1432-1033.1984.tb08445.x; BOLAND W, 1995, ANGEW CHEM INT EDIT, V34, P1602, DOI 10.1002/anie.199516021; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Frigerio M, 1999, J ORG CHEM, V64, P4537, DOI 10.1021/jo9824596; GARDNER HW, 1984, J ORG CHEM, V49, P508, DOI 10.1021/jo00177a024; GERWICK WH, 1993, CHEM REV, V93, P1807, DOI 10.1021/cr00021a008; GERWICK WH, 1993, HYDROBIOLOGIA, V261, P653; Habel A, 2007, J CHROMATOGR A, V1165, P182, DOI 10.1016/j.chroma.2007.07.051; Hombeck M, 1999, CHEM COMMUN, P243, DOI 10.1039/a808409b; HUYNH C, 1979, TETRAHEDRON LETT, P1503; Maier I, 1995, PROGR PHYCOL RES, V11, P51; More JD, 2002, ORG LETT, V4, P3001, DOI 10.1021/ol026427n; MULLER DG, 1994, EUR J PHYCOL, V29, P219, DOI 10.1080/09670269400650671; NEUMANN C, 1990, EUR J BIOCHEM, V191, P453, DOI 10.1111/j.1432-1033.1990.tb19143.x; Nubbemeyer U, 2003, SYNTHESIS-STUTTGART, P961; OSBORN JA, 1966, J CHEM SOC A, P1711, DOI 10.1039/j19660001711; Ozkan I, 2003, J ORG CHEM, V68, P9635, DOI 10.1021/jo035173w; Pohnert G, 2004, CHEM PHYS LIPIDS, V131, P159, DOI 10.1016/j.chemphyslip.2004.04.011; Pohnert G, 2000, EUR J ORG CHEM, V2000, P1821; Pohnert G, 2002, NAT PROD REP, V19, P108, DOI 10.1039/a806888g; Pohnert G, 1996, TETRAHEDRON, V52, P10073, DOI 10.1016/0040-4020(96)00548-0; Pohnert G, 1997, TETRAHEDRON, V53, P13681, DOI 10.1016/S0040-4020(97)00886-7; Rickert KW, 1999, BIOCHEMISTRY-US, V38, P12218, DOI 10.1021/bi990834y; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Rodriguez-Ruiz J, 1998, BIOTECHNOL TECH, V12, P689, DOI 10.1023/A:1008812904017; Schaus SE, 2002, J AM CHEM SOC, V124, P1307, DOI 10.1021/ja016737l; SCHMID CE, 1994, J PLANT PHYSIOL, V143, P570, DOI 10.1016/S0176-1617(11)81826-2; Schnitzler I, 2001, OECOLOGIA, V126, P515, DOI 10.1007/s004420000546; Spiteller G, 1999, J CHROMATOGR A, V843, P29, DOI 10.1016/S0021-9673(98)01078-4; STOWELL JC, 1979, SYNTHESIS-STUTTGART, P132; STRATMANN K, 1992, ANGEW CHEM INT EDIT, V31, P1246, DOI 10.1002/anie.199212461; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; Thottumkara AP, 2005, ORG LETT, V7, P2933, DOI 10.1021/ol050875o; THYMAN JHP, 1996, SYNTHESIS LIPID CHEM; TROST BM, 1995, ANGEW CHEM INT EDIT, V34, P259, DOI 10.1002/anie.199502591; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG	38	12	13	1	22	AMER CHEMICAL SOC	WASHINGTON	1155 16TH ST, NW, WASHINGTON, DC 20036 USA	0022-3263	1520-6904		J ORG CHEM	J. Org. Chem.	JUN 18	2010	75	12					3958	3964		10.1021/jo1004372			7	Chemistry, Organic	Chemistry	608CW	WOS:000278560700003	20504036				2021-04-07	
J	Cock, JM; Sterck, L; Rouze, P; Scornet, D; Allen, AE; Amoutzias, G; Anthouard, V; Artiguenave, F; Aury, JM; Badger, JH; Beszteri, B; Billiau, K; Bonnet, E; Bothwell, JH; Bowler, C; Boyen, C; Brownlee, C; Carrano, CJ; Charrier, B; Cho, GY; Coelho, SM; Collen, J; Corre, E; Da Silva, C; Delage, L; Delaroque, N; Dittami, SM; Doulbeau, S; Elias, M; Farnham, G; Gachon, CMM; Gschloessl, B; Heesch, S; Jabbari, K; Jubin, C; Kawai, H; Kimura, K; Kloareg, B; Kupper, FC; Lang, D; Le Bail, A; Leblanc, C; Lerouge, P; Lohr, M; Lopez, PJ; Martens, C; Maumus, F; Michel, G; Miranda-Saavedra, D; Morales, J; Moreau, H; Motomura, T; Nagasato, C; Napoli, CA; Nelson, DR; Nyvall-Collen, P; Peters, AF; Pommier, C; Potin, P; Poulain, J; Quesneville, H; Read, B; Rensing, SA; Ritter, A; Rousvoal, S; Samanta, M; Samson, G; Schroeder, DC; Segurens, B; Strittmatter, M; Tonon, T; Tregear, JW; Valentin, K; von Dassow, P; Yamagishi, T; Van de Peer, Y; Wincker, P				Cock, J. Mark; Sterck, Lieven; Rouze, Pierre; Scornet, Delphine; Allen, Andrew E.; Amoutzias, Grigoris; Anthouard, Veronique; Artiguenave, Francois; Aury, Jean-Marc; Badger, Jonathan H.; Beszteri, Bank; Billiau, Kenny; Bonnet, Eric; Bothwell, John H.; Bowler, Chris; Boyen, Catherine; Brownlee, Colin; Carrano, Carl J.; Charrier, Benedicte; Cho, Ga Youn; Coelho, Susana M.; Collen, Jonas; Corre, Erwan; Da Silva, Corinne; Delage, Ludovic; Delaroque, Nicolas; Dittami, Simon M.; Doulbeau, Sylvie; Elias, Marek; Farnham, Garry; Gachon, Claire M. M.; Gschloessl, Bernhard; Heesch, Svenja; Jabbari, Kamel; Jubin, Claire; Kawai, Hiroshi; Kimura, Kei; Kloareg, Bernard; Kuepper, Frithjof C.; Lang, Daniel; Le Bail, Aude; Leblanc, Catherine; Lerouge, Patrice; Lohr, Martin; Lopez, Pascal J.; Martens, Cindy; Maumus, Florian; Michel, Gurvan; Miranda-Saavedra, Diego; Morales, Julia; Moreau, Herve; Motomura, Taizo; Nagasato, Chikako; Napoli, Carolyn A.; Nelson, David R.; Nyvall-Collen, Pi; Peters, Akira F.; Pommier, Cyril; Potin, Philippe; Poulain, Julie; Quesneville, Hadi; Read, Betsy; Rensing, Stefan A.; Ritter, Andres; Rousvoal, Sylvie; Samanta, Manoj; Samson, Gaelle; Schroeder, Declan C.; Segurens, Beatrice; Strittmatter, Martina; Tonon, Thierry; Tregear, James W.; Valentin, Klaus; von Dassow, Peter; Yamagishi, Takahiro; Van de Peer, Yves; Wincker, Patrick			The Ectocarpus genome and the independent evolution of multicellularity in brown algae	NATURE			English	Article							RECEPTOR-LIKE KINASES; LAMINARIA-DIGITATA; GENE FAMILY; PHAEOPHYCEAE; SILICULOSUS; PROPAGATION; EUKARYOTES; PATTERNS; BLADES; PLANTS	Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related(1). These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. Brown algae are also one of only a small number of eukaryotic lineages that have evolved complex multicellularity (Fig. 1). We report the 214 million base pair (Mbp) genome sequence of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for brown algae(2-5), closely related to the kelps(6,7) (Fig. 1). Genome features such as the presence of an extended set of light-harvesting and pigment biosynthesis genes and new metabolic processes such as halide metabolism help explain the ability of this organism to cope with the highly variable tidal environment. The evolution of multicellularity in this lineage is correlated with the presence of a rich array of signal transduction genes. Of particular interest is the presence of a family of receptor kinases, as the independent evolution of related molecules has been linked with the emergence of multicellularity in both the animal and green plant lineages. The Ectocarpus genome sequence represents an important step towards developing this organism as a model species, providing the possibility to combine genomic and genetic(2) approaches to explore these and other(4,5) aspects of brown algal biology further.	[Cock, J. Mark; Scornet, Delphine; Boyen, Catherine; Charrier, Benedicte; Cho, Ga Youn; Coelho, Susana M.; Collen, Jonas; Delage, Ludovic; Dittami, Simon M.; Gschloessl, Bernhard; Heesch, Svenja; Kloareg, Bernard; Le Bail, Aude; Leblanc, Catherine; Michel, Gurvan; Nyvall-Collen, Pi; Peters, Akira F.; Potin, Philippe; Ritter, Andres; Rousvoal, Sylvie; Tonon, Thierry] Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, F-29682 Roscoff, France; [Cock, J. Mark; Scornet, Delphine; Boyen, Catherine; Charrier, Benedicte; Cho, Ga Youn; Coelho, Susana M.; Collen, Jonas; Delage, Ludovic; Dittami, Simon M.; Gschloessl, Bernhard; Heesch, Svenja; Kloareg, Bernard; Le Bail, Aude; Leblanc, Catherine; Michel, Gurvan; Nyvall-Collen, Pi; Peters, Akira F.; Potin, Philippe; Ritter, Andres; Rousvoal, Sylvie; Tonon, Thierry] CNRS, UMR 7139, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, F-29682 Roscoff, France; [Sterck, Lieven; Rouze, Pierre; Amoutzias, Grigoris; Billiau, Kenny; Bonnet, Eric; Martens, Cindy; Van de Peer, Yves] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium; [Sterck, Lieven; Rouze, Pierre; Amoutzias, Grigoris; Billiau, Kenny; Bonnet, Eric; Martens, Cindy; Van de Peer, Yves] Univ Ghent, Dept Plant Biotechnol & Genet, B-9052 Ghent, Belgium; [Allen, Andrew E.; Badger, Jonathan H.] J Craig Venter Inst, San Diego, CA 92121 USA; [Anthouard, Veronique; Artiguenave, Francois; Aury, Jean-Marc; Da Silva, Corinne; Jabbari, Kamel; Jubin, Claire; Poulain, Julie; Samson, Gaelle; Segurens, Beatrice; Wincker, Patrick] CEA, DSV, Inst Genom, F-91057 Evry, France; [Beszteri, Bank; Valentin, Klaus] Alfred Wegener Inst Polar & Marine Res, D-27570 Bremerhaven, Germany; [Bothwell, John H.] Queens Univ Belfast, Sch Biol Sci, Belfast BT9 7BL, Antrim, North Ireland; [Bothwell, John H.] Queens Univ, Marine Lab, Portaferry BT22 1PF, Co Down, North Ireland; [Bothwell, John H.; Brownlee, Colin; Farnham, Garry; Schroeder, Declan C.] Marine Biol Assoc United Kingdom Lab, Plymouth PL1 2PB, Devon, England; [Bowler, Chris; Jabbari, Kamel; Lopez, Pascal J.; Maumus, Florian] Ecole Normale Super, CNRS, UMR8197, Inst Biol, F-75005 Paris, France; [Bowler, Chris] Stn Zool, I-80121 Naples, Italy; [Carrano, Carl J.] San Diego State Univ, San Diego, CA 92182 USA; [Corre, Erwan] Comp & Genom Resource Ctr, FR 2424, Stn Biol Roscoff, F-29682 Roscoff, France; [Delaroque, Nicolas] Fraunhofer Inst Cell Therapy & Immunol IZI, D-04103 Leipzig, Germany; [Doulbeau, Sylvie; Tregear, James W.] IRD, CIRAD Palm Dev Biol Grp, UMR DIAPC 1097, F-34394 Montpellier, France; [Elias, Marek] Charles Univ Prague, Fac Sci, Dept Bot, Prague 1280 12, Czech Republic; [Elias, Marek] Charles Univ Prague, Dept Parasitol, Prague 1280 12, Czech Republic; [Gachon, Claire M. M.; Kuepper, Frithjof C.; Strittmatter, Martina] Scottish Assoc Marine Sci, Dept Microbial & Mol Biol, Scottish Marine Inst, Oban PA37 1QA, Argyll, Scotland; [Kawai, Hiroshi; Yamagishi, Takahiro] Kobe Univ, Res Ctr Inland Seas, Nada Ku, Kobe, Hyogo 6578501, Japan; [Kimura, Kei; Motomura, Taizo; Nagasato, Chikako] Hokkaido Univ, Field Sci Ctr No Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510003, Japan; [Lang, Daniel; Rensing, Stefan A.] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany; [Lerouge, Patrice] Univ Rouen, IFRMP 23, Lab Glyco MEV EA 4358, F-76821 Mont St Aignan, France; [Lohr, Martin] Johannes Gutenberg Univ Mainz, Inst Allgemeine Bot, D-55099 Mainz, Germany; [Miranda-Saavedra, Diego] Univ Cambridge, Cambridge Inst Med Res, Dept Haematol, Cambridge, England; [Morales, Julia] Univ Paris 06, UMR Mer & Sante 7150, Equipe Traduct Cycle Cellulaire & Dev, Stn Biol Roscoff, F-29680 Roscoff, France; [Morales, Julia] CNRS, UMR Mer & Sante 7150, Stn Biol Roscoff, F-29680 Roscoff, France; [Moreau, Herve] Lab Arago, F-66651 Banyuls Sur Mer, France; [Napoli, Carolyn A.] Univ Arizona, Inst Bio5, Tucson, AZ 85719 USA; [Napoli, Carolyn A.] Univ Arizona, Dept Plant Sci, Tucson, AZ 85719 USA; [Nelson, David R.] Univ Tennessee, Hlth Sci Ctr, Dept Mol Sci, Memphis, TN 38163 USA; [Pommier, Cyril; Quesneville, Hadi] INRA, Unite Rech Genom Info UR INRA 1164, Ctr Rech Versailles, F-78026 Versailles, France; [Read, Betsy] Cal State Univ, San Marcos, CA 92096 USA; [Ritter, Andres] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Ctr Adv Studies Ecol & Biodivers, Santiago, Chile; [von Dassow, Peter] CNRS, UMR 7144, Stn Biol Roscoff, F-29682 Roscoff, France; [Samanta, Manoj] Systemix Inst, Redmond, WA 98053 USA	Cock, JM (corresponding author), Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, Pl Georges Teissier,BP74, F-29682 Roscoff, France.	cock@sb-roscoff.fr	Elias, Marek/D-6851-2014; Yamagishi, Toshio/E-2384-2013; corre, erwan/O-4669-2019; Valentin, Klaus/G-5862-2014; Nagasato, Chikako/A-3392-2012; Lang, Daniel/C-7238-2008; Bowler, Chris/AAC-6256-2019; Van de Peer, Yves/D-4388-2009; Schroeder, Declan C./O-9131-2019; Tonon, Thierry/A-3214-2009; Aury, Jean-Marc/N-1621-2019; Van de Peer, Yves/AAE-7666-2019; Sterck, Lieven/A-9439-2016; amoutzias, Grigoris/O-4565-2019; Lang, Daniel/M-5597-2019; MICHEL, Gurvan/B-3490-2008; Beszteri, Bank M/D-1961-2010; Miranda-Saavedra, Diego/C-2707-2012; Gachon, Claire/C-2787-2009; Lohr, Martin/A-1214-2009; Dittami, Simon/E-8354-2011; Coelho, Susana/ABH-8166-2020; Lopez, Pascal Jean/P-5813-2019; von Dassow, Peter/A-5399-2012; Maumus, Florian/O-5426-2016; Tregear, James/E-6954-2011; Quesneville, Hadi/AAG-1048-2020; Lopez, Pascal Jean/A-7427-2011; Allen, Andrew E/C-4896-2012	Elias, Marek/0000-0003-0066-6542; Yamagishi, Toshio/0000-0002-8890-1115; corre, erwan/0000-0001-6354-2278; Valentin, Klaus/0000-0001-7401-9423; Lang, Daniel/0000-0002-2166-0716; Van de Peer, Yves/0000-0003-4327-3730; Schroeder, Declan C./0000-0001-5991-2838; Tonon, Thierry/0000-0002-1454-6018; Aury, Jean-Marc/0000-0003-1718-3010; Van de Peer, Yves/0000-0003-4327-3730; Sterck, Lieven/0000-0001-7116-4000; amoutzias, Grigoris/0000-0001-5961-964X; Lang, Daniel/0000-0002-2166-0716; Beszteri, Bank M/0000-0002-6852-1588; Lohr, Martin/0000-0002-4335-9887; Dittami, Simon/0000-0001-7987-7523; Lopez, Pascal Jean/0000-0002-9914-4252; Maumus, Florian/0000-0001-7325-0527; Quesneville, Hadi/0000-0003-3001-4908; Allen, Andrew E/0000-0001-5911-6081; Charrier, Benedicte/0000-0001-5721-1640; Heesch, Svenja/0000-0002-4531-0921; DA SILVA, Corinne/0000-0002-7618-7831; POTIN, Philippe/0000-0001-7358-6282; MICHEL, Gurvan/0000-0002-3009-6205; von Dassow, Peter/0000-0002-1858-1953; Bowler, Chris/0000-0003-3835-6187; Bonnet, Eric/0000-0002-8468-2867; Strittmatter, Martina/0000-0002-1258-9751; Gachon, Claire/0000-0002-3702-7472; Pommier, Cyril/0000-0002-9040-8733; Morales, Julia/0000-0003-1632-5419; Poulain, Julie/0000-0002-8744-3116; Peters, Akira/0000-0001-5332-199X; Cock, J. Mark/0000-0002-2650-0383; Kuepper, Frithjof/0000-0003-1273-7109; Nelson, David/0000-0003-0583-5421; Ritter, Andres/0000-0001-7011-6824	French GIS 'Institut de la Genomique Marine'; Centre National de Recherche Scientifique; European UnionEuropean Commission; GIS Europole Mer; Inter-University Network for Fundamental Research [P6/25]; 'Conseil General' of the Finistere department; University Pierre and Marie Curie; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [21657018, 22370024, 20370025, 21770065] Funding Source: KAKEN; Biotechnology and Biological Sciences Research CouncilUK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC) [P18266, REI20579] Funding Source: researchfish; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [mba010003, NE/F012578/1, NE/D521522/1] Funding Source: researchfish	We would like to thank Dieter G. Muller for his help and advice. The project was supported by the French GIS 'Institut de la Genomique Marine', the Centre National de Recherche Scientifique, the European Union network of excellence Marine Genomics Europe, the GIS Europole Mer, the Inter-University Network for Fundamental Research (P6/25, BioMaGNet), the 'Conseil General' of the Finistere department and the University Pierre and Marie Curie.	Arnaout MA, 2007, CURR OPIN CELL BIOL, V19, P495, DOI 10.1016/j.ceb.2007.08.002; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2002, CURR OPIN CELL BIOL, V14, P230, DOI 10.1016/S0955-0674(02)00305-8; Coelho SM, 2002, PLANT CELL, V14, P2369, DOI 10.1105/tpc.003285; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Colin C, 2003, J BIOL CHEM, V278, P23545, DOI 10.1074/jbc.M300247200; De Smet I, 2009, NAT CELL BIOL, V11, P1166, DOI 10.1038/ncb1009-1166; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dixon NM, 2000, PHYCOLOGIA, V39, P258, DOI 10.2216/i0031-8884-39-3-258.1; Goddard H, 2000, P NATL ACAD SCI USA, V97, P1932, DOI 10.1073/pnas.020516397; Griffiths-Jones S, 2008, NUCLEIC ACIDS RES, V36, pD154, DOI 10.1093/nar/gkm952; Kawai H, 2007, J PHYCOL, V43, P186, DOI 10.1111/j.1529-8817.2006.00308.x; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Lin ZG, 2006, P NATL ACAD SCI USA, V103, P10328, DOI 10.1073/pnas.0604232103; Marin I, 2008, FASEB J, V22, P3103, DOI 10.1096/fj.08-111310; Muller DG, 2000, BOT MAR, V43, P157, DOI 10.1515/BOT.2000.016; Nakayama Y, 2007, P NATL ACAD SCI USA, V104, P5883, DOI 10.1073/pnas.0609996104; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peterson KJ, 2009, BIOESSAYS, V31, P736, DOI 10.1002/bies.200900033; Phillips N, 2008, J PHYCOL, V44, P394, DOI 10.1111/j.1529-8817.2008.00473.x; Rozema J, 2002, J PHOTOCH PHOTOBIO B, V66, P2, DOI 10.1016/S1011-1344(01)00269-X; RUSSELL G, 1983, MAR ECOL PROG SER, V13, P303, DOI 10.3354/meps013303; RUSSELL G, 1983, MAR ECOL PROG SER, V11, P181, DOI 10.3354/meps011181; Shiu SH, 2001, P NATL ACAD SCI USA, V98, P10763, DOI 10.1073/pnas.181141598; Wheeler GL, 2008, TRENDS PLANT SCI, V13, P506, DOI 10.1016/j.tplants.2008.06.004; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075	29	542	581	2	232	NATURE PUBLISHING GROUP	LONDON	MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	0028-0836	1476-4687		NATURE	Nature	JUN 3	2010	465	7298					617	621		10.1038/nature09016			5	Multidisciplinary Sciences	Science & Technology - Other Topics	604AF	WOS:000278249000042	20520714	Green Published, Other Gold	Y	N	2021-04-07	
J	Ritter, A; Ubertini, M; Romac, S; Gaillard, F; Delage, L; Mann, A; Cock, JM; Tonon, T; Correa, JA; Potin, P				Ritter, Andres; Ubertini, Martin; Romac, Sarah; Gaillard, Fanny; Delage, Ludovic; Mann, Aaron; Cock, J. Mark; Tonon, Thierry; Correa, Juan A.; Potin, Philippe			Copper stress proteomics highlights local adaptation of two strains of the model brown alga Ectocarpus siliculosus	PROTEOMICS			English	Article						2-DE; Algae; Bromoperoxidase; Copper; Plant proteomics; Stress	MARINE FOULING ALGA; HEAVY-METAL DETOXIFICATION; LAMINARIA-DIGITATA; OXIDATIVE STRESS; PHOTOSYSTEM-II; PROTEIN; ARABIDOPSIS; TOLERANCE; RESPONSES; GLUTATHIONE	Ectocarpus siliculosus is a cosmopolitan brown alga with capacity to thrive in copper enriched environments. Analysis of copper toxicity was conducted in two strains of E. siliculosus isolated from (i) an uncontaminated coast in southern Peru (Es32) and (ii) a copper polluted rocky beach in northern Chile (Es524). Es32 was more sensitive than Es524, with toxicity detected at 50 mu g/L Cu, whereas Es524 displayed negative effects only when exposed to 250 mu g/L Cu. Differential soluble proteome profiling for each strain exposed to sub-lethal copper levels allowed to identify the induction of proteins related to processes such as energy production, glutathione metabolism as well as accumulation of HSPs. In addition, the inter-strain comparison of stress-related proteomes led to identify features related to copper tolerance in Es524, such as striking expression of a PSII Mn-stabilizing protein and a Fucoxanthine chlorophyll a c binding protein. Es524 also expressed specific stress-related enzymes such as RNA helicases from the DEAD box families and a vanadium-dependent bromoperoxidase. These observations were supported by RT-qPCR for some of the identified genes and an enzyme activity assay for vanadium-dependent bromoperoxidase. Therefore, the occurrence of two different phenotypes within two distinct E. siliculosus strains studied at the physiological and proteomic levels strongly suggest that persistent copper stress may represent a selective force leading to the development of strains genetically adapted to copper contaminated sites.	[Potin, Philippe] Univ Paris 06, CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France; [Ritter, Andres; Mann, Aaron; Correa, Juan A.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Adv Studies Ecol & Biodivers, Dept Ecol, Santiago, Chile; [Romac, Sarah; Gaillard, Fanny] CNRS, Biol Stn, Comp & Genom Resource Ctr, Roscoff, France	Potin, P (corresponding author), Univ Paris 06, CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, Pl Georges Teissier,BP74, F-29682 Roscoff, France.	potin@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; Ubertini, Martin/AAH-3898-2020	Tonon, Thierry/0000-0002-1454-6018; POTIN, Philippe/0000-0001-7358-6282; Ritter, Andres/0000-0001-7011-6824; Cock, J. Mark/0000-0002-2650-0383	EUEuropean Commission [GOCE-CT-2004-505403]; French Embassy and the CONICYT of Chile; Laboratoire International Associe "Dispersal and Adaptation of Marine Species" (LIA DIAMS) PUC, Chile; CNRS-UPMC, France; FONDAP [1501-0001]; ICA	This work has been partially funded by Marine Genomics Europe NoE 7 (EU contract no. GOCE-CT-2004-505403) and by the French Embassy and the CONICYT of Chile through PhD fellowships to A. R. This work was supported by the Laboratoire International Associe "Dispersal and Adaptation of Marine Species" (LIA DIAMS) PUC, Chile and CNRS-UPMC, France. Additional support came from FONDAP 1501-0001 (Program 7) and ICA research grant, both to J. A. C. We are especially grateful to Jessica Beltran and Santiago Andrade for valuable suggestions.	ALSCHER RG, 1989, PHYSIOL PLANTARUM, V77, P457, DOI 10.1111/j.1399-3054.1989.tb05667.x; ANDRADE LR, 2002, PHYCOLOGIA, V41, P39, DOI DOI 10.2216/I0031-8884-41-1-39.1; Andres-Colas N, 2006, PLANT J, V45, P225, DOI 10.1111/j.1365-313X.2005.02601.x; BAKER AJM, 1990, PLANT SYST EVOL, V173, P91, DOI 10.1007/BF00937765; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Bernal M, 2004, PHYSIOL PLANTARUM, V120, P686, DOI 10.1111/j.1399-3054.2004.0286.x; Bona E, 2007, PROTEOMICS, V7, P1121, DOI 10.1002/pmic.200600712; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; Braun RJ, 2008, BBA-MOL CELL RES, V1783, P1418, DOI 10.1016/j.bbamcr.2008.01.015; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; CID A, 1995, AQUAT TOXICOL, V31, P165, DOI 10.1016/0166-445X(94)00071-W; Clemens S, 2006, BIOCHIMIE, V88, P1707, DOI 10.1016/j.biochi.2006.07.003; Clendennen SK, 1996, J PHYCOL, V32, P614, DOI 10.1111/j.0022-3646.1996.00614.x; Cobbett C, 2002, ANNU REV PLANT BIOL, V53, P159, DOI 10.1146/annurev.arplant.53.100301.135154; COCK JM, 2010, NATURE IN PRESS, DOI DOI 10.1038/NATURE09016; Colin C, 2005, J BIOL INORG CHEM, V10, P156, DOI 10.1007/s00775-005-0626-8; Contreras L, 2005, J PHYCOL, V41, P1184, DOI 10.1111/j.1529-8817.2005.00151.x; Contreras L, 2008, J PHYCOL, V44, P1315, DOI 10.1111/j.1529-8817.2008.00575.x; Correa JA, 1999, J APPL PHYCOL, V11, P57, DOI 10.1023/A:1008027610826; de Franco PO, 2008, MAR GENOM, V1, P135, DOI 10.1016/j.margen.2009.01.003; FERNANDES JC, 1991, BOT REV, V57, P246, DOI 10.1007/BF02858564; Gledhill M, 1997, J PHYCOL, V33, P2, DOI 10.1111/j.0022-3646.1997.00002.x; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; HALL A, 1980, NEW PHYTOL, V85, P73, DOI 10.1111/j.1469-8137.1980.tb04449.x; Hall JL, 2002, J EXP BOT, V53, P1, DOI 10.1093/jexbot/53.366.1; HALLIWELL B, 1992, FEBS LETT, V307, P108, DOI 10.1016/0014-5793(92)80911-Y; HALLIWELL B, 1984, BIOCHEM J, V219, P1, DOI 10.1042/bj2190001; Halliwell B, 2006, PLANT PHYSIOL, V141, P312, DOI 10.1104/pp.106.077073; Henmi T, 2004, PLANT CELL PHYSIOL, V45, P243, DOI 10.1093/pcp/pch027; KAREZ CS, 1995, BOT MAR, V38, P151, DOI 10.1515/botm.1995.38.1-6.151; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kupper H, 2005, MET IONS BIOL SYST, V44, P97; Kung CCS, 2006, PROTEOMICS, V6, P2746, DOI 10.1002/pmic.200500108; Kupper FC, 1998, PLANTA, V207, P163, DOI 10.1007/s004250050469; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Leblanc C, 2006, BIOCHIMIE, V88, P1773, DOI 10.1016/j.biochi.2006.09.001; Lee SE, 2006, PROTEOMICS, V6, P945, DOI 10.1002/pmic.200401349; Liao MT, 2000, PLANT SOIL, V223, P245, DOI 10.1023/A:1004843505053; LINDQUIST S, 1988, ANNU REV GENET, V22, P631, DOI 10.1146/annurev.ge.22.120188.003215; Livingstone DR, 2001, MAR POLLUT BULL, V42, P656, DOI 10.1016/S0025-326X(01)00060-1; Luo Y, 2009, J PLANT PHYSIOL, V166, P385, DOI 10.1016/j.jplph.2008.06.018; MACNAIR MR, 1993, NEW PHYTOL, V124, P541, DOI 10.1111/j.1469-8137.1993.tb03846.x; Maksymiec W, 1997, PHOTOSYNTHETICA, V34, P321, DOI 10.1023/A:1006818815528; Medina M, 2005, MAR POLLUT BULL, V50, P396, DOI 10.1016/j.marpolbul.2004.11.022; Medina MH, 2007, CHEMOSPHERE, V67, P2105, DOI 10.1016/j.chemosphere.2006.12.024; MEISTER A, 1983, ANNU REV BIOCHEM, V52, P711, DOI 10.1146/annurev.bi.52.070183.003431; Morris CA, 1999, BIOCHEM J, V338, P553, DOI 10.1042/0264-6021:3380553; MORRIS OP, 1974, J BRIT PHYCOL, V9, P269; Nadimpalli R, 2000, J BIOL CHEM, V275, P29579, DOI 10.1074/jbc.M002339200; Panaretakis T, 2008, CELL DEATH DIFFER, V15, P1499, DOI 10.1038/cdd.2008.67; Pauwels M, 2008, CURR OPIN PLANT BIOL, V11, P129, DOI 10.1016/j.pbi.2008.01.005; Pawlik-Skowronska B, 2007, AQUAT TOXICOL, V83, P190, DOI 10.1016/j.aquatox.2007.04.003; Pearl LH, 2006, ANNU REV BIOCHEM, V75, P271, DOI 10.1146/annurev.biochem.75.103004.142738; Pinto E, 2003, J PHYCOL, V39, P1008, DOI 10.1111/j.0022-3646.2003.02-193.x; Puig S, 2007, PLANT CELL ENVIRON, V30, P271, DOI 10.1111/j.1365-3040.2007.01642.x; Requejo R, 2005, PHYTOCHEMISTRY, V66, P1519, DOI 10.1016/j.phytochem.2005.05.003; Rijstenbil JW, 2003, MAR ECOL PROG SER, V254, P37, DOI 10.3354/meps254037; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Rocak S, 2004, NAT REV MOL CELL BIO, V5, P232, DOI 10.1038/nrm1335; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Salgado LT, 2005, PROTOPLASMA, V225, P123, DOI 10.1007/s00709-004-0066-2; Sanan-Mishra N, 2005, P NATL ACAD SCI USA, V102, P509, DOI 10.1073/pnas.0406485102; Sarry JE, 2006, PROTEOMICS, V6, P2180, DOI 10.1002/pmic.200500543; Smith AP, 2004, J BIOL CHEM, V279, P26098, DOI 10.1074/jbc.M402807200; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; SUEUR S, 1982, LIMNOL OCEANOGR, V27, P536, DOI 10.4319/lo.1982.27.3.0536; Toth G, 2000, MAR ECOL PROG SER, V192, P119, DOI 10.3354/meps192119; Verhaeghe EF, 2008, J BIOL INORG CHEM, V13, P257, DOI 10.1007/s00775-007-0319-6; VIERLING E, 1991, ANNU REV PLANT PHYS, V42, P579, DOI 10.1146/annurev.pp.42.060191.003051; Xiang CB, 1998, PLANT CELL, V10, P1539, DOI 10.1105/tpc.10.9.1539; Yruela I, 1996, J BIOL CHEM, V271, P27408, DOI 10.1074/jbc.271.44.27408; Yruela I, 2000, PHYSIOL PLANTARUM, V110, P551, DOI 10.1111/j.1399-3054.2000.1100419.x	73	67	69	0	42	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	1615-9853	1615-9861		PROTEOMICS	Proteomics	JUN	2010	10	11					2074	2088		10.1002/pmic.200900004			15	Biochemical Research Methods; Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	611PK	WOS:000278831700001	20373519				2021-04-07	
J	Le Bail, A; Billoud, B; Kowalczyk, N; Kowalczyk, M; Gicquel, M; Le Panse, S; Stewart, S; Scornet, D; Cock, JM; Ljung, K; Charrier, B				Le Bail, Aude; Billoud, Bernard; Kowalczyk, Nathalie; Kowalczyk, Mariusz; Gicquel, Morgane; Le Panse, Sophie; Stewart, Sarah; Scornet, Delphine; Cock, Jeremy Mark; Ljung, Karin; Charrier, Benedicte			Auxin Metabolism and Function in the Multicellular Brown Alga Ectocarpus siliculosus	PLANT PHYSIOLOGY			English	Article							ACTIN CYTOSKELETON; FUCUS-DISTICHUS; CELL FATE; BIOSYNTHESIS; ARABIDOPSIS; POLARITY; PHAEOPHYCEAE; EXPRESSION; GENERATION; EVOLUTION	Ectocarpus siliculosus is a small brown alga that has recently been developed as a genetic model. Its thallus is filamentous, initially organized as a main primary filament composed of elongated cells and round cells, from which branches differentiate. Modeling of its early development suggests the involvement of very local positional information mediated by cell-cell recognition. However, this model also indicates that an additional mechanism is required to ensure proper organization of the branching pattern. In this paper, we show that auxin indole-3-acetic acid (IAA) is detectable in mature E. siliculosus organisms and that it is present mainly at the apices of the filaments in the early stages of development. An in silico survey of auxin biosynthesis, conjugation, response, and transport genes showed that mainly IAA biosynthesis genes from land plants have homologs in the E. siliculosus genome. In addition, application of exogenous auxins and 2,3,5-triiodobenzoic acid had different effects depending on the developmental stage of the organism, and we propose a model in which auxin is involved in the negative control of progression in the developmental program. Furthermore, we identified an auxin-inducible gene called EsGRP1 from a small-scale microarray experiment and showed that its expression in a series of morphogenetic mutants was positively correlated with both their elongated-to-round cell ratio and their progression in the developmental program. Altogether, these data suggest that IAA is used by the brown alga Ectocarpus to relay cell-cell positional information and induces a signaling pathway different from that known in land plants.	[Le Bail, Aude; Billoud, Bernard; Kowalczyk, Nathalie; Gicquel, Morgane; Stewart, Sarah; Scornet, Delphine; Cock, Jeremy Mark; Charrier, Benedicte] Univ Paris 06, CNRS, UMR Marine Plants & Biomol 7139, F-29682 Roscoff, France; [Le Panse, Sophie] CNRS, Biol Stn, FR2424, F-29682 Roscoff, France; [Kowalczyk, Mariusz; Ljung, Karin] Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, S-90183 Umea, Sweden	Charrier, B (corresponding author), Univ Paris 06, CNRS, UMR Marine Plants & Biomol 7139, F-29682 Roscoff, France.	charrier@sb-roscoff.fr	Ljung, Karin/AAE-8691-2019; Kowalczyk, Mariusz/ABD-2910-2020	Ljung, Karin/0000-0003-2901-189X; Kowalczyk, Mariusz/0000-0001-7454-4762; Cock, J. Mark/0000-0002-2650-0383; Billoud, Bernard/0000-0002-5140-8087; Charrier, Benedicte/0000-0001-5721-1640	French Ministry of National Education and Research; French Groupement d'Interet Scientifique	This work was supported by the French Ministry of National Education and Research (to A. L. B.) and the French Groupement d'Interet Scientifique "Europole Mer."	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Avsian-Kretchmer O, 2002, PLANT PHYSIOL, V130, P199, DOI 10.1104/pp.003228; Bairoch A, 2009, NUCLEIC ACIDS RES, V37, pD169, DOI 10.1093/nar/gkn664; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Basu S, 2002, PLANT PHYSIOL, V130, P292, DOI 10.1104/pp.004747; BERGER F, 1994, SCIENCE, V263, P1421, DOI 10.1126/science.263.5152.1421; Bierfreund NM, 2003, PLANT CELL REP, V21, P1143, DOI 10.1007/s00299-003-0646-1; Billoud B, 2008, FUNCT PLANT BIOL, V35, P1014, DOI 10.1071/FP08036; Bouget FY, 1998, DEVELOPMENT, V125, P1999; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; BRADLEY PM, 1991, J PHYCOL, V27, P317, DOI 10.1111/j.0022-3646.1991.00317.x; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cooke TJ, 2002, PLANT MOL BIOL, V49, P319, DOI 10.1023/A:1015242627321; Cove D, 2000, J PLANT GROWTH REGUL, V19, P275, DOI 10.1007/s003440000031; Cove D, 2006, ANNU REV PLANT BIOL, V57, P497, DOI 10.1146/annurev.arplant.57.032905.105338; Dhonukshe P, 2008, P NATL ACAD SCI USA, V105, P4489, DOI 10.1073/pnas.0711414105; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Friml J, 2010, EUR J CELL BIOL, V89, P231, DOI 10.1016/j.ejcb.2009.11.003; Holtkamp AD, 2009, APPL MICROBIOL BIOT, V82, P1, DOI 10.1007/s00253-008-1790-x; Abad MJ, 2008, MINI-REV MED CHEM, V8, P740, DOI 10.2174/138955708784912148; Kai T, 2006, J APPL PHYCOL, V18, P95, DOI 10.1007/s10811-005-9020-8; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; Kepinski S, 2007, BIOESSAYS, V29, P953, DOI 10.1002/bies.20657; KIELISZEWSKI MJ, 1994, PLANT J, V5, P157, DOI 10.1046/j.1365-313X.1994.05020157.x; Klarzynski O, 2000, PLANT PHYSIOL, V124, P1027, DOI 10.1104/pp.124.3.1027; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; KROPF DL, 1993, DEV BIOL, V160, P303, DOI 10.1006/dbio.1993.1309; KROPF DL, 1992, MICROBIOL REV, V56, P316, DOI 10.1128/MMBR.56.2.316-339.1992; Kropf DL, 1997, PLANT CELL, V9, P1011, DOI 10.1105/tpc.9.7.1011; Lau S, 2008, PLANT CELL, V20, P1738, DOI 10.1105/tpc.108.060418; Lau S, 2009, TRENDS PLANT SCI, V14, P182, DOI 10.1016/j.tplants.2009.01.004; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Ljung K, 2002, PLANT MOL BIOL, V49, P249, DOI 10.1023/A:1015298812300; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Nafisi M, 2007, PLANT CELL, V19, P2039, DOI 10.1105/tpc.107.051383; Overbeek R, 1999, P NATL ACAD SCI USA, V96, P2896, DOI 10.1073/pnas.96.6.2896; Paciorek T, 2005, NATURE, V435, P1251, DOI 10.1038/nature03633; Parry G, 2006, CURR OPIN CELL BIOL, V18, P152, DOI 10.1016/j.ceb.2006.02.001; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Phillips N, 2008, J PHYCOL, V44, P394, DOI 10.1111/j.1529-8817.2008.00473.x; Ringli C, 2001, CELL MOL LIFE SCI, V58, P1430, DOI 10.1007/PL00000786; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stepanova AN, 2008, CELL, V133, P177, DOI 10.1016/j.cell.2008.01.047; Stern CD, 2006, CURR OPIN GENET DEV, V16, P413, DOI 10.1016/j.gde.2006.06.005; Sugawara S, 2009, P NATL ACAD SCI USA, V106, P5430, DOI 10.1073/pnas.0811226106; Sun HG, 2004, PLANT PHYSIOL, V135, P266, DOI 10.1104/pp.103.034900; Tarakhovskaya ER, 2007, RUSS J PLANT PHYSL+, V54, P163, DOI 10.1134/S1021443707020021; Titapiwatanakun B, 2009, PLANT J, V57, P27, DOI 10.1111/j.1365-313X.2008.03668.x; Woodward AW, 2005, ANN BOT-LONDON, V95, P707, DOI 10.1093/aob/mci083; Yamamoto M, 1998, PLANT CELL PHYSIOL, V39, P660, DOI 10.1093/oxfordjournals.pcp.a029419; YAMASHITA Y, 1998, P 2 INT C KNOWL BAS, V3, P2; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Zdobnov EM, 2001, BIOINFORMATICS, V17, P847, DOI 10.1093/bioinformatics/17.9.847	57	65	67	0	21	AMER SOC PLANT BIOLOGISTS	ROCKVILLE	15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA	0032-0889	1532-2548		PLANT PHYSIOL	Plant Physiol.	MAY	2010	153	1					128	144		10.1104/pp.109.149708			17	Plant Sciences	Plant Sciences	590PS	WOS:000277239900010	20200071	Green Published, Bronze			2021-04-07	
J	Parodi, ER; Caceres, EJ; Westermeier, R; Muller, DG				Parodi, Elisa R.; Caceres, Eduardo J.; Westermeier, Renato; Mueller, Dieter G.			Secondary zoospores in the algal endoparasite Maullinia ectocarpii (Plasmodiophoromycota)	BIOCELL			English	Article						intracellular parasites; flagellar apparatus; Plasmodiophoromycota; brown algae; Ectocarpales	FLAGELLAR APPARATUS; ELECTRON-MICROSCOPY; POLYMYXA-GRAMINIS; ULTRASTRUCTURE; SPONGOSPORA	The present paper deals with the ultrastructure of zoospores produced by the plasmodiophorid ectocarpii, living in the marine algal host Ectocarpus silicalosus. The zoospores described here are very similar to secondary zoospores of Polymyxa graminis and Phagomyxa sp. (the latter an algal endoparasite, also). Our results indicate that M. ectocarpii produces two types of plasmodia, and suggest that is a species with a complete life cycle, as it is known for all the Plasmodiophormycota that have been studied. Sporogenic and sporangial plasmodia produce, respectively, primary zoospores with parallel flagella within thick walled resting sporangia, and secondary zoospores with opposite flagella within thin walled sporangia.	[Parodi, Elisa R.] Univ Nacl Sur, Lab Ecol Acuat, Dept Biol Bioquim & Farm, RA-8000 Bahia Blanca, Buenos Aires, Argentina; [Parodi, Elisa R.] Consejo Nacl Invest Cient & Tecn, CCTBB, Inst Argentino Oceanog IADO, RA-8000 Bahia Blanca, Buenos Aires, Argentina; [Caceres, Eduardo J.] Univ Nacl Sur, Lab Ficol & Micol, Dept Biol Bioquim & Farm, RA-8000 Bahia Blanca, Buenos Aires, Argentina; [Westermeier, Renato] Univ Austral Chile, Fac Pesquerias & Oceanog, Puerto Montt, Chile; [Mueller, Dieter G.] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany	Parodi, ER (corresponding author), Univ Nacl Sur, Lab Ecol Acuat, Dept Biol Bioquim & Farm, RA-8000 Bahia Blanca, Buenos Aires, Argentina.	eparodi@criba.edu.ar			Secretaria de Ciencia y Tecnologia de la Universidad Nacional del Sur [CSU- 24/B145]; National Research Council (CONICET)Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP 277/00]	This research was supported by grants from the Secretaria de Ciencia y Tecnologia de la Universidad Nacional del Sur (PGI CSU- 24/B145) and from the National Research Council (CONICET) (PIP 277/00). ERP is Research Member of the Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET) and EJC is Research Member of the Comision de Investigaciones Cientificas de la Provincia de Buenos Aires, Argentina (CIC).	Alexopoulos C., 1996, INTRO MYCOLOGY; Barr D. J. S., 1979, Canadian Journal of Plant Pathology, V1, P85; BARR DJS, 1982, CAN J BOT, V60, P2496, DOI 10.1139/b82-302; BRASELTON JP, 1995, CRIT REV MICROBIOL, V21, P263, DOI 10.3109/10408419509113543; Cavalier-Smith T, 2002, INT J SYST EVOL MICR, V52, P297, DOI 10.1099/00207713-52-2-297; Clay CM, 1997, MYCOL RES, V101, P737, DOI 10.1017/S0953756296003401; Dick MW, 1997, MYCOL RES, V101, P385, DOI 10.1017/S0953756296003267; Down GJ, 2002, MYCOL RES, V106, P1060, DOI 10.1017/S0953756202006391; DYLEWSKI P, 1990, HDB PROTOCTISTA, P399; HARDHAM AR, 1987, PROTOPLASMA, V137, P109, DOI 10.1007/BF01281146; Kanyuka K, 2003, MOL PLANT PATHOL, V4, P393, DOI 10.1046/J.1364-3703.2003.00177.X; LANGE L, 1976, PROTOPLASMA, V89, P339, DOI 10.1007/BF01275750; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; MILLER CE, 1985, CAN J BOT, V63, P263, DOI 10.1139/b85-030; Prillinger H, 2002, CHEM IMMUNOL, V81, P207; REYMOND OL, 1983, J MICROSC-OXFORD, V130, P79, DOI 10.1111/j.1365-2818.1983.tb04200.x; SCHNEPF E, 1994, BOT ACTA, V107, P374, DOI 10.1111/j.1438-8677.1994.tb00810.x; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; TALLEY MR, 1978, MYCOLOGIA, V70, P1241, DOI 10.2307/3759323; TEAKLE DS, 1983, ZOOSPORIC PLANT PATH, P233; TEAKLE DS, 1980, VECTORS PLANT PATHOG, P151	21	7	7	0	12	INST HISTOL EMBRIOL-CONICET	MENDOZA	FAC CIENCIAS MED-UNIV NAC CUYO CASILLA DE CORREO 56, 5500 MENDOZA, ARGENTINA	0327-9545			BIOCELL	Biocell	APR	2010	34	1					45	52					8	Biology	Life Sciences & Biomedicine - Other Topics	597GJ	WOS:000277744300006	20506630				2021-04-07	
J	Peters, AF; Mann, AD; Cordova, CA; Brodie, J; Correa, JA; Schroeder, DC; Cock, JM				Peters, Akira F.; Mann, Aaron D.; Cordova, Cesar A.; Brodie, Juliet; Correa, Juan A.; Schroeder, Declan C.; Cock, J. Mark			Genetic diversity of Ectocarpus (Ectocarpales, Phaeophyceae) in Peru and northern Chile, the area of origin of the genome-sequenced strain	NEW PHYTOLOGIST			English	Article						Chile; Ectocarpus; genetic diversity; Kuckuckia; Peru	GENUS ECTOCARPUS; SILICULOSUS; GENERATION; BRITAIN	P>The origin of the Ectocarpus strain used for genome sequencing (the 'genome strain') was Peru, where no Ectocarpus had been reported previously. To study the genetic diversity in the region and to increase the number of individuals from this area available for genetic experiments, 119 new Ectocarpus strains were isolated from eight localities along the 3000 km of coastline from central Peru to central Chile. Internal transcribed spacer 1 (ITS1) genotyping revealed nine different genotypes, five of which were endemic to the area studied and three of which were previously unknown. Individuals of the same genotype as the genome strain occurred from Peru to northernmost Chile, representing 61% of the samples in this area, from which five more genotypes were isolated. Further south, down to central Chile, most individuals belonged to Ectocarpus siliculosus, Ectocarpus fasciculatus and Ectocarpus crouaniorum. In sexual crosses, the genome strain and the new isolates of the same genotype were fully compatible. Sequences from four nuclear and cytoplasmic genetic markers (ITS1, ITS2, Rubisco spacer and Cytochrome-c oxidase subunit 3 (cox3)) separated the genome strain from the known species of Ectocarpus. It may in future be recognized as a separate species.	[Peters, Akira F.; Schroeder, Declan C.] Marine Biol Assoc UK, Plymouth PL1 2PB, Devon, England; [Peters, Akira F.] Bezhin Rosko, F-29680 Roscoff, France; [Peters, Akira F.; Cock, J. Mark] CNRS, Biol Stn, UMR7139, F-29682 Roscoff, France; [Peters, Akira F.; Cock, J. Mark] Univ Paris 06, F-29682 Roscoff, France; [Mann, Aaron D.; Correa, Juan A.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Santiago 340, Chile; [Mann, Aaron D.; Correa, Juan A.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Adv Studies Ecol & Biodivers, Santiago 340, Chile; [Cordova, Cesar A.] Univ Nacl Mayor San Marcos, Fac Ciencias Biol, Lima 14, Peru; [Brodie, Juliet] Nat Hist Museum, Dept Bot, London SW7 5BD, England	Peters, AF (corresponding author), Marine Biol Assoc UK, Citadel Hill, Plymouth PL1 2PB, Devon, England.	akirapeters@gmail.com	Schroeder, Declan C./O-9131-2019	Schroeder, Declan C./0000-0001-5991-2838; Cock, J. Mark/0000-0002-2650-0383; Peters, Akira/0000-0001-5332-199X	Universite Pierre et Marie Curie; Darwin Initiative Pre-project [EIDPR35]; CONICYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) [FONDAP 1501 0001]; ICA; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [MBA010001] Funding Source: researchfish	We thank Cesar Acleto for permission to study specimens in the phycological herbarium at the Museo de Historia Natural, Lima, and Matt Hall for advice during the molecular experiments. A.F.P. was supported by a travel grant from Universite Pierre et Marie Curie for the collections and by a Ray Lankester Investigatorship by the Marine Biological Association of the United Kingdom for the molecular work. J.B. was supported by a Darwin Initiative Pre-project grant (Darwin Reference: EIDPR35). Collections and isolations in Chile were supported by FONDAP 1501 0001 (CONICYT) awarded to the Center for Advanced Studies in Ecology & Biodiversity (CASEB) Program 7. Additional support to J.A.C. was provided by an ICA grant.	Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Andrade S, 2006, MAR CHEM, V101, P203, DOI 10.1016/j.marchem.2006.03.002; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; COCK JM, 2010, NATURE IN PRESS; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Felsenstein J., 1995, PHYLIP PHYLOGENY INF; Graham MH, 2007, P NATL ACAD SCI USA, V104, P16576, DOI 10.1073/pnas.0704778104; HAMEL G, 1939, BOT NOTISER, P65; Heesch S, 2010, NEW PHYTOL, V188, DOI 10.1111/j.1469-8137.2010.03273.x; HOWE M.A., 1914, MEM TORREY BOT CLUB, V15, P1; Muller D. G., 1991, JPN J PHYCOL, V39, P151; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Page RDM, 1996, COMPUT APPL BIOSCI, V12, P357; PEDERSEN PM, 1989, NORD J BOT, V9, P443, DOI 10.1111/j.1756-1051.1989.tb01024.x; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; PETERS AF, 1993, MAR BIOL, V115, P143, DOI 10.1007/BF00349396; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Peters AF, 2010, PHYCOL RES, V58, P157, DOI 10.1111/j.1440-1835.2010.00574.x; RAMBAUT A, 2002, SE A1 V2 0A11; Ramirez M. E., 1991, CATALOGO ALGAS MARIN; Ritter A, 2010, PROTEOMICS, V10, P2074, DOI 10.1002/pmic.200900004; RUSSELL G, 1967, J MAR BIOL ASSOC UK, V47, P233, DOI 10.1017/S0025315400033695; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; Santelices B., 1989, ALGAS MARINAS CHILE; Setchell WA, 1925, U CALIF PUBL BOT, V8, P383; SETCHELL WA, 1922, U CALIFORNIA PUBLICA, V11, P403; STACHE B, 1989, EVOLUTIONARY BIOGEOG, P173; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Tanaka A, 2010, EUR J PHYCOL, V45, P107, DOI 10.1080/09670260903383271; Yoshida T, 1998, MARINE ALGAE JAPAN; Yoshida Tadao, 1995, Japanese Journal of Phycology, V43, P115	33	17	17	0	8	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X	1469-8137		NEW PHYTOL	New Phytol.		2010	188	1					30	41		10.1111/j.1469-8137.2010.03303.x			12	Plant Sciences	Plant Sciences	646OI	WOS:000281551500009	20524993	Bronze			2021-04-07	
J	Rayko, E; Maumus, F; Maheswari, U; Jabbari, K; Bowler, C				Rayko, Edda; Maumus, Florian; Maheswari, Uma; Jabbari, Kamel; Bowler, Chris			Transcription factor families inferred from genome sequences of photosynthetic stramenopiles	NEW PHYTOLOGIST			English	Article						diatom; Ectocarpus; expression analysis; haptophyte; heat shock transcription factor; heterokont; Myb transcription factor; stramenopile; stress; transcription factor; transposable elements; zinc finger	DIATOM THALASSIOSIRA-PSEUDONANA; DNA-BINDING PROTEIN; FACTOR DATABASE; ARABIDOPSIS; MYB; GENE; DOMAIN; DIVERSIFICATION; PHOTORECEPTOR; COMPONENTS	P>By comparative analyses we identify lineage-specific diversity in transcription factors (TFs) from stramenopile (or heterokont) genome sequences. We compared a pennate (Phaeodactylum tricornutum) and a centric diatom (Thalassiosira pseudonana) with those of other stramenopiles (oomycetes, Pelagophyceae, and Phaeophyceae (Ectocarpus siliculosus)) as well as to that of Emiliania huxleyi, a haptophyte that is evolutionarily related to the stramenopiles. We provide a detailed description of diatom TF complements and report numerous peculiarities: in both diatoms, the heat shock factor (HSF) family is overamplified and constitutes the most abundant class of TFs; Myb and C2H2-type zinc finger TFs are the two most abundant TF families encoded in all the other stramenopile genomes investigated; the presence of diatom and lineage-specific gene fusions, in particular a class of putative photoreceptors with light-sensitive Per-Arnt-Sim (PAS) and DNA-binding (basic-leucine zipper, bZIP) domains and an HSF-AP2 domain fusion. Expression data analysis shows that many of the TFs studied are transcribed and may be involved in specific responses to environmental stimuli. Evolutionary and functional relevance of these observations are discussed.	[Rayko, Edda; Maumus, Florian; Maheswari, Uma; Jabbari, Kamel; Bowler, Chris] Ecole Normale Super, INSERM, U1024, Inst Biol,CNRS,UMR8197, F-75005 Paris, France	Bowler, C (corresponding author), Ecole Normale Super, INSERM, U1024, Inst Biol,CNRS,UMR8197, 46 Rue Ulm, F-75005 Paris, France.	cbowler@biologie.ens.fr	Bowler, Chris/AAC-6256-2019; Maumus, Florian/O-5426-2016	Bowler, Chris/0000-0003-3835-6187; Shunmugam, Uma/0000-0001-7007-9234; Maumus, Florian/0000-0001-7325-0527	EUEuropean Commission; Agence Nationale de la Recherche (France)French National Research Agency (ANR)	We thank A. E. Allen for verifying the existence of the Pt HSF-AP2 transcript by reverse transcriptase- PCR. Funding for this work was from the EU-funded Diatomics project and the Agence Nationale de la Recherche (France).	Adams MD, 2000, SCIENCE, V287, P2185, DOI 10.1126/science.287.5461.2185; Armbrust EV, 2009, NATURE, V459, P185, DOI 10.1038/nature08057; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; BARANOWSKIJ N, 1994, EMBO J, V13, P5383, DOI 10.1002/j.1460-2075.1994.tb06873.x; BHATTACHARYA D, 1993, MOL BIOL EVOL, V10, P689; BORGSTAHL GEO, 1995, BIOCHEMISTRY-US, V34, P6278, DOI 10.1021/bi00019a004; BOWLER C, 2009, ANNU REV MAR SCI, V2, P429; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Chen YH, 2006, PLANT MOL BIOL, V60, P107, DOI 10.1007/s11103-005-2910-y; Crosson S, 2003, BIOCHEMISTRY-US, V42, P2, DOI 10.1021/bi026978l; Diaz-Martin J, 2005, PLANT PHYSIOL, V139, P1483, DOI 10.1104/pp.105.069963; Eisen MB, 1998, P NATL ACAD SCI USA, V95, P14863, DOI 10.1073/pnas.95.25.14863; Enright AJ, 1999, NATURE, V402, P86; Field CB, 1998, SCIENCE, V281, P237, DOI 10.1126/science.281.5374.237; Guo AY, 2008, NUCLEIC ACIDS RES, V36, pD966, DOI 10.1093/nar/gkm841; HURST HC, 1995, PROTEIN PROFILE, V2, P105; Ishikawa M, 2009, PLANTA, V230, P543, DOI 10.1007/s00425-009-0967-6; Ito M, 2005, J PLANT RES, V118, P61, DOI 10.1007/s10265-005-0192-8; Jakoby M, 2002, TRENDS PLANT SCI, V7, P106, DOI 10.1016/S1360-1385(01)02223-3; Keeling PJ, 2010, PHILOS T R SOC B, V365, P729, DOI 10.1098/rstb.2009.0103; Kewley RJ, 2004, INT J BIOCHEM CELL B, V36, P189, DOI 10.1016/S1357-2725(03)00211-5; Levine M, 2003, NATURE, V424, P147, DOI 10.1038/nature01763; Lipsick JS, 1996, ONCOGENE, V13, P223; LITTLEWOOD TD, 1995, PROTEIN PROFILE, V2, P621; Maheswari U, 2009, NUCLEIC ACIDS RES, V37, pD1001, DOI 10.1093/nar/gkn905; Marchler-Bauer A, 2002, NUCLEIC ACIDS RES, V30, P281, DOI 10.1093/nar/30.1.281; Maumus F, 2009, BMC GENOMICS, V10, DOI 10.1186/1471-2164-10-624; Mayr BM, 2005, J BIOL CHEM, V280, P15103, DOI 10.1074/jbc.M414144200; Montsant A, 2007, J PHYCOL, V43, P585, DOI 10.1111/j.1529-8817.2007.00342.x; MORIMOTO RI, 1992, J BIOL CHEM, V267, P21987; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; NOMURA N, 1988, NUCLEIC ACIDS RES, V16, P11075, DOI 10.1093/nar/16.23.11075; Nover L, 2001, CELL STRESS CHAPERON, V6, P177, DOI 10.1379/1466-1268(2001)006<0177:AATHST>2.0.CO;2; OHI R, 1994, EMBO J, V13, P471, DOI 10.1002/j.1460-2075.1994.tb06282.x; Perez-Rodriguez P, 2010, NUCLEIC ACIDS RES, V38, pD822, DOI 10.1093/nar/gkp805; Riano-Pachon DM, 2008, GENETICS, V179, P31, DOI 10.1534/genetics.107.086090; Riechmann JL, 2000, SCIENCE, V290, P2105, DOI 10.1126/science.290.5499.2105; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Saldanha AJ, 2004, BIOINFORMATICS, V20, P3246, DOI 10.1093/bioinformatics/bth349; Sali A, 1999, NATURE, V402, P23, DOI 10.1038/46915; Schaffer R, 1998, CELL, V93, P1219, DOI 10.1016/S0092-8674(00)81465-8; Schaffer R, 2001, PLANT CELL, V13, P113, DOI 10.1105/tpc.13.1.113; Shamovsky I, 2008, CELL MOL LIFE SCI, V65, P855, DOI 10.1007/s00018-008-7458-y; Song CP, 2005, PLANT CELL, V17, P2384, DOI 10.1105/tpc.105.033043; SORGER PK, 1988, CELL, V54, P855, DOI 10.1016/S0092-8674(88)91219-6; Stracke R, 2001, CURR OPIN PLANT BIOL, V4, P447, DOI 10.1016/S1369-5266(00)00199-0; Takahashi F, 2007, P NATL ACAD SCI USA, V104, P19625, DOI 10.1073/pnas.0707692104; Thompson JD, 1997, NUCLEIC ACIDS RES, V25, P4876, DOI 10.1093/nar/25.24.4876; Vardi A, 2006, PLOS BIOL, V4, P411, DOI 10.1371/journal.pbio.0040060; Wang ZY, 1997, PLANT CELL, V9, P491, DOI 10.1105/tpc.9.4.491; Wu C, 1995, ANNU REV CELL DEV BI, V11, P441, DOI 10.1146/annurev.cb.11.110195.002301; Yu EY, 2000, J BIOL CHEM, V275, P24208, DOI 10.1074/jbc.M003250200	52	63	63	1	34	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X	1469-8137		NEW PHYTOL	New Phytol.		2010	188	1					52	66		10.1111/j.1469-8137.2010.03371.x			15	Plant Sciences	Plant Sciences	646OI	WOS:000281551500010	20646219	Bronze			2021-04-07	
J	Michel, G; Tonon, T; Scornet, D; Cock, JM; Kloareg, B				Michel, Gurvan; Tonon, Thierry; Scornet, Delphine; Cock, J. Mark; Kloareg, Bernard			Central and storage carbon metabolism of the brown alga Ectocarpus siliculosus: insights into the origin and evolution of storage carbohydrates in Eukaryotes	NEW PHYTOLOGIST			English	Article						beta-1; 3-glucan; brown algae; Chromalveolate; Eukaryotic evolution; glycogen; mannitol; starch; trehalose	TREHALOSE METABOLISM; STARCH SYNTHESIS; GENOME SEQUENCE; RED; PROTEIN; LAMINARIN; MANNITOL; MODEL; IDENTIFICATION; DINOFLAGELLATE	P>Brown algae exhibit a unique carbon (C) storage metabolism. The photoassimilate d-fructose 6-phosphate is not used to produce sucrose but is converted into d-mannitol. These seaweeds also store C as beta-1,3-glucan (laminarin), thus markedly departing from most living organisms, which use alpha-1,4-glucans (glycogen or starch). Using a combination of bioinformatic and phylogenetic approaches, we identified the candidate genes for the enzymes involved in C storage in the genome of the brown alga Ectocarpus siliculosus and traced their evolutionary origins. Ectocarpus possesses a complete set of enzymes for synthesis of mannitol, laminarin and trehalose. By contrast, the pathways for sucrose, starch and glycogen are completely absent. The synthesis of beta-1,3-glucans appears to be a very ancient eukaryotic pathway. Brown algae inherited the trehalose pathway from the red algal progenitor of phaeoplasts, while the mannitol pathway was acquired by lateral gene transfer from Actinobacteria. The starch metabolism of the red algal endosymbiont was entirely lost in the ancestor of Stramenopiles. In light of these novel findings we question the validity of the 'Chromalveolate hypothesis'.	[Michel, Gurvan; Tonon, Thierry; Scornet, Delphine; Cock, J. Mark; Kloareg, Bernard] Univ Paris 06, UMR Marine Plants & Biomol 7139, Stn Biol Roscoff, F-29682 Roscoff, Bretagne, France; [Michel, Gurvan; Tonon, Thierry; Scornet, Delphine; Cock, J. Mark; Kloareg, Bernard] CNRS, UMR Marine Plants & Biomol 7139, Stn Biol Roscoff, F-29682 Roscoff, Bretagne, France	Michel, G (corresponding author), Univ Paris 06, UMR Marine Plants & Biomol 7139, Stn Biol Roscoff, F-29682 Roscoff, Bretagne, France.	gurvan@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; MICHEL, Gurvan/B-3490-2008	Tonon, Thierry/0000-0002-1454-6018; Cock, J. Mark/0000-0002-2650-0383; MICHEL, Gurvan/0000-0002-3009-6205			Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; ASSALI NE, 1991, PLANT MOL BIOL, V17, P853, DOI 10.1007/BF00037066; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Ball SG, 2003, ANNU REV PLANT BIOL, V54, P207, DOI 10.1146/annurev.arplant.54.031902.134927; BARTNICK.S, 1968, ANNU REV MICROBIOL, V22, P87, DOI 10.1146/annurev.mi.22.100168.000511; Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkr1065, 10.1093/nar/gkp985]; BEATTIE A, 1961, BIOCHEM J, V79, P531, DOI 10.1042/bj0790531; Ben Ali A, 2001, INT J SYST EVOL MICR, V51, P737, DOI 10.1099/00207713-51-3-737; Bodyl A, 2009, TRENDS ECOL EVOL, V24, P119, DOI 10.1016/j.tree.2008.11.003; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Bull AT, 2005, ANTON LEEUW INT J G, V87, P65, DOI 10.1007/s10482-004-6562-8; Cantarel BL, 2009, NUCLEIC ACIDS RES, V37, pD233, DOI 10.1093/nar/gkn663; Cardenas ML, 1998, BBA-MOL CELL RES, V1401, P242, DOI 10.1016/S0167-4889(97)00150-X; Cavalier-Smith T, 1999, J EUKARYOT MICROBIOL, V46, P347, DOI 10.1111/j.1550-7408.1999.tb04614.x; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; Coppin A, 2005, J MOL EVOL, V60, P257, DOI 10.1007/s00239-004-0185-6; Dagan T, 2009, SCIENCE, V324, P1651, DOI 10.1126/science.1175765; Dauvillee D, 2009, P NATL ACAD SCI USA, V106, P21126, DOI 10.1073/pnas.0907424106; Derelle E, 2006, P NATL ACAD SCI USA, V103, P11647, DOI 10.1073/pnas.0604795103; Deschamps P, 2008, MOL BIOL EVOL, V25, P536, DOI 10.1093/molbev/msm280; Douzery EJP, 2004, P NATL ACAD SCI USA, V101, P15386, DOI 10.1073/pnas.0403984101; Edner C, 2007, PLANT PHYSIOL, V145, P17, DOI 10.1104/pp.107.104224; Elbein AD, 2003, GLYCOBIOLOGY, V13, p17R, DOI 10.1093/glycob/cwg047; Gil R, 2004, ENVIRON MICROBIOL, V6, P1109, DOI 10.1111/j.1462-2920.2004.00691.x; Goddijn OJM, 1999, TRENDS PLANT SCI, V4, P315, DOI 10.1016/S1360-1385(99)01446-6; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Haas BJ, 2009, NATURE, V461, P393, DOI 10.1038/nature08358; HENRISSAT B, 1991, BIOCHEM J, V280, P309, DOI 10.1042/bj2800309; Henrissat B, 2002, TRENDS GENET, V18, P437, DOI 10.1016/S0168-9525(02)02734-8; Henrissat B, 2001, PLANT MOL BIOL, V47, P55, DOI 10.1023/A:1010667012056; IKAWA T, 1972, PLANT CELL PHYSIOL, V13, P1017; Iturriaga G, 2009, INT J MOL SCI, V10, P3793, DOI 10.3390/ijms10093793; Iwamoto K, 2005, MAR BIOTECHNOL, V7, P407, DOI 10.1007/s10126-005-0029-4; Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436; Keeling PJ, 2009, J EUKARYOT MICROBIOL, V56, P1, DOI 10.1111/j.1550-7408.2008.00371.x; KREMER BP, 1980, BRIT PHYCOL J, V15, P399, DOI 10.1080/00071618000650401; Krogh A, 2001, J MOL BIOL, V305, P567, DOI 10.1006/jmbi.2000.4315; Kumar S, 2004, BRIEF BIOINFORM, V5, P150, DOI 10.1093/bib/5.2.150; Lairson LL, 2008, ANNU REV BIOCHEM, V77, P521, DOI 10.1146/annurev.biochem.76.061005.092322; Liaud MF, 2000, MOL BIOL EVOL, V17, P213, DOI 10.1093/oxfordjournals.molbev.a026301; Liberator P, 1998, J BIOL CHEM, V273, P4237, DOI 10.1074/jbc.273.7.4237; Lobban CS, 1994, SEAWEED ECOLOGY PHYS; Matsuzaki M, 2004, NATURE, V428, P653, DOI 10.1038/nature02398; Merchant SS, 2007, SCIENCE, V318, P245, DOI 10.1126/science.1143609; Michel G, 2010, NEW PHYTOL, V188, P82, DOI 10.1111/j.1469-8137.2010.03374.x; Montijn RC, 1999, J BACTERIOL, V181, P7414, DOI 10.1128/JB.181.24.7414-7420.1999; Moulin P, 1999, J PHYCOL, V35, P1237, DOI 10.1046/j.1529-8817.1999.3561237.x; Moustafa A, 2009, SCIENCE, V324, P1724, DOI 10.1126/science.1172983; Nyvall P, 1999, PLANTA, V209, P143, DOI 10.1007/s004250050616; PARKER BC, 1965, J PHYCOL, V1, P172, DOI 10.1111/j.1529-8817.1965.tb04579.x; Paul MJ, 2008, ANNU REV PLANT BIOL, V59, P417, DOI 10.1146/annurev.arplant.59.032607.092945; Pearson GA, 2010, MAR BIOTECHNOL, V12, P195, DOI 10.1007/s10126-009-9208-z; PERCIVAL EGV, 1951, J CHEM SOC, P720, DOI 10.1039/jr9510000720; Plancke C, 2008, EUKARYOT CELL, V7, P247, DOI 10.1128/EC.00373-07; Read SM, 1996, CARBOHYD RES, V281, P187, DOI 10.1016/0008-6215(95)00350-9; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; RICHTER DFE, 1987, PLANTA, V170, P528, DOI 10.1007/BF00402987; Ridder IS, 1999, BIOCHEM J, V339, P223, DOI 10.1042/0264-6021:3390223; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Salerno GL, 2003, TRENDS PLANT SCI, V8, P63, DOI 10.1016/S1360-1385(02)00029-8; SCHMITZ K, 1976, MAR BIOL, V36, P207, DOI 10.1007/BF00389281; Tyler BM, 2006, SCIENCE, V313, P1261, DOI 10.1126/science.1128796; Viola R, 2001, P ROY SOC B-BIOL SCI, V268, P1417, DOI 10.1098/rspb.2001.1644; WANG MC, 1974, CARBOHYD RES, V37, P331, DOI 10.1016/S0008-6215(00)82922-5; Wong TKM, 2007, J PHYCOL, V43, P528, DOI 10.1111/j.1529-8817.2007.00349.x; Worden AZ, 2009, SCIENCE, V324, P268, DOI 10.1126/science.1167222; YAMAGUCHI T, 1966, PLANT CELL PHYSIOL, V7, P217	69	115	118	2	66	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X	1469-8137		NEW PHYTOL	New Phytol.		2010	188	1					67	81		10.1111/j.1469-8137.2010.03345.x			15	Plant Sciences	Plant Sciences	646OI	WOS:000281551500011	20618908				2021-04-07	
J	Gravot, A; Dittami, SM; Rousvoal, S; Lugan, R; Eggert, A; Collen, J; Boyen, C; Bouchereau, A; Tonon, T				Gravot, Antoine; Dittami, Simon M.; Rousvoal, Sylvie; Lugan, Raphael; Eggert, Anja; Collen, Jonas; Boyen, Catherine; Bouchereau, Alain; Tonon, Thierry			Diurnal oscillations of metabolite abundances and gene analysis provide new insights into central metabolic processes of the brown alga Ectocarpus siliculosus	NEW PHYTOLOGIST			English	Article						brown algae (Phaeophyceae); carbon-concentrating mechanisms; Ectocarpus siliculosus; metabolite profiling; nyctemeral cycle; photorespiration; transcriptomic profiling; gamma-aminobutyric acid (GABA)	LOW-CARBON AVAILABILITY; BLUE-LIGHT; INORGANIC CARBON; AMINO-ACIDS; GAMMA-AMINOBUTYRATE; SEASONAL-VARIATIONS; BETA-CARBOXYLATION; MARINE MACROALGAE; ABIOTIC STRESS; BUFFER SYSTEM	P>Knowledge about primary metabolic processes is essential for the understanding of the physiology and ecology of seaweeds. The Ectocarpus siliculosus genome now facilitates integrative studies of the molecular basis of primary metabolism in this brown alga. Metabolite profiling was performed across two light-dark cycles and under different CO(2) and O(2) concentrations, together with genome and targeted gene expression analysis. Except for mannitol, E. siliculosus cells contain low levels of polyols, organic acids and carbohydrates. Amino acid profiles were similar to those of C3-type plants, including glycine/serine accumulation under photorespiration-enhancing conditions. gamma-Aminobutyric acid was only detected in traces. Changes in the concentrations of glycine and serine, genome annotation and targeted expression analysis together suggest the presence of a classical photorespiratory glycolate pathway in E. siliculosus rather than a malate synthase pathway as in diatoms. Several metabolic and transcriptional features do not clearly fit with the hypothesis of an alanine/aspartate-based inducible C4-like metabolism in E. siliculosus. We propose a model in which the accumulation of alanine could be used to store organic carbon and nitrogen during the light period. We finally discuss a possible link between low gamma-aminobutyric acid contents and the absence of glutamate decarboxylase genes in the Ectocarpus genome.	[Dittami, Simon M.; Rousvoal, Sylvie; Collen, Jonas; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France; [Gravot, Antoine; Lugan, Raphael; Bouchereau, Alain] Univ Rennes 1, INRA, UMR 118, F-35042 Rennes, France; [Dittami, Simon M.; Rousvoal, Sylvie; Collen, Jonas; Boyen, Catherine; Tonon, Thierry] CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France; [Eggert, Anja] Leibniz Inst Balt Sea Res Warnemunde Phys Oceanog, D-18119 Rostock, Germany	Tonon, T (corresponding author), Univ Paris 06, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France.	tonon@sb-roscoff.fr	Dittami, Simon/E-8354-2011; Tonon, Thierry/A-3214-2009	Dittami, Simon/0000-0001-7987-7523; Tonon, Thierry/0000-0002-1454-6018	Rennes MetropoleRegion Bretagne; European communityEuropean Commission [MESTCT 2005-020737]	We would like to thank Constance de Villardi for practical help in setting up the carbon starvation and enrichment experiments, Jean-Paul Guegan (ENSCR, Rennes, France) for technical assistance with the NMR experiments, and Francois Larher and Hugues Renault for helpful discussions. Rennes Metropole is acknowledged for its financial support for the acquisition of the UPLC equipment. S.D. received funding from the European community's Sixth Framework Programme (ESTeam contract no MESTCT 2005-020737).	Adams MA, 1999, ANAL BIOCHEM, V266, P77, DOI 10.1006/abio.1998.2906; AKAGAWA H, 1972, BOT MAR, V15, P119, DOI 10.1515/botm.1972.15.3.119; Allan WL, 2006, CAN J BOT, V84, P1339, DOI 10.1139/B06-093; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P771, DOI 10.1111/j.1365-3040.1989.tb01638.x; AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P765, DOI 10.1111/j.1365-3040.1989.tb01637.x; Baldauf SL, 2008, J SYST EVOL, V46, P263, DOI 10.3724/SP.J.1002.2008.08008; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; BURRIS JE, 1977, MAR BIOL, V39, P371, DOI 10.1007/BF00391940; Busch S, 2001, EUR J PHYCOL, V36, P61, DOI 10.1080/09670260110001735208; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Clark SM, 2009, J EXP BOT, V60, P3255, DOI 10.1093/jxb/erp161; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; DAVISON IR, 1985, PHYCOLOGIA, V24, P449, DOI 10.2216/i0031-8884-24-4-449.1; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Forde BG, 2007, J EXP BOT, V58, P2339, DOI 10.1093/jxb/erm121; Fox J, 2005, J BEHAV EDUC, V14, P1, DOI 10.1007/s10864-005-0957-0; Foyer CH, 2003, J EXP BOT, V54, P585, DOI 10.1093/jxb/erg053; Foyer CH, 2009, ANNU REV PLANT BIOL, V60, P455, DOI 10.1146/annurev.arplant.043008.091948; Gagneul D, 2007, PLANT PHYSIOL, V144, P1598, DOI 10.1104/pp.107.099820; Gibon Y, 2006, GENOME BIOL, V7, DOI 10.1186/gb-2006-7-8-r76; Granum E, 2009, J PHYCOL, V45, P1083, DOI 10.1111/j.1529-8817.2009.00728.x; GROSS W, 1990, J PHYCOL, V26, P381, DOI 10.1111/j.0022-3646.1990.00381.x; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Hillrichs S, 2001, EUR J PHYCOL, V36, P71, DOI 10.1017/S096702620100302X; Igarashi D, 2006, PLANT PHYSIOL, V142, P901, DOI 10.1104/pp.106.085514; Iwamoto Koji, 1997, Phycological Research, V45, P77, DOI 10.1111/j.1440-1835.1997.tb00066.x; JOHNSTON AM, 1991, CAN J BOT, V69, P1123, DOI 10.1139/b91-144; JONES RF, 1956, BIOL BULL, V110, P169, DOI 10.2307/1538978; Jubault M, 2008, PLANT PHYSIOL, V146, P2008, DOI 10.1104/pp.108.117432; KREMER BP, 1980, PLANTA, V150, P189, DOI 10.1007/BF00582365; KREMER BP, 1977, PLANTA, V133, P191, DOI 10.1007/BF00391918; Kroth PG, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0001426; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lugan R, 2009, PLANT CELL ENVIRON, V32, P95, DOI 10.1111/j.1365-3040.2008.01898.x; Michel G, 2010, NEW PHYTOL, V188, P67, DOI 10.1111/j.1469-8137.2010.03345.x; Moulin P, 1999, J PHYCOL, V35, P1237, DOI 10.1046/j.1529-8817.1999.3561237.x; NAGAHISA E, 1995, BIOSCI BIOTECH BIOCH, V59, P2176, DOI 10.1271/bbb.59.2176; NASR A H, 1967, Hydrobiologia, V29, P80, DOI 10.1007/BF00142055; Neuberger G, 2003, J MOL BIOL, V328, P581, DOI 10.1016/S0022-2836(03)00319-X; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Petroff OAC, 2002, NEUROSCIENTIST, V8, P562, DOI 10.1177/1073858402238515; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; ROSELL KG, 1985, J PHYCOL, V21, P304, DOI 10.1111/j.0022-3646.1985.00304.x; Saeed AI, 2003, BIOTECHNIQUES, V34, P374, DOI 10.2144/03342mt01; Schmid R, 1998, PLANT CELL ENVIRON, V21, P523, DOI 10.1046/j.1365-3040.1998.00297.x; Schmid R, 2001, EUR J PHYCOL, V36, P257, DOI 10.1017/S0967026201003274; Schmid R, 1996, SCI MAR, V60, P115; SMITH DG, 1955, J BIOL CHEM, V217, P845; Starr RC, 1993, J PHYCOL S, V29, P90; VALLE EM, 1991, PLANT PHYSIOL, V95, P839, DOI 10.1104/pp.95.3.839; WINKLER U, 1995, PLANTA, V195, P403; YAMAGUCHI T, 1966, PLANT CELL PHYSIOL, V7, P217; Young EB, 2007, PLANT CELL ENVIRON, V30, P764, DOI 10.1111/j.1365-3040.2007.01666.x	54	62	63	2	53	WILEY-BLACKWELL	MALDEN	COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA	0028-646X			NEW PHYTOL	New Phytol.		2010	188	1					98	110		10.1111/j.1469-8137.2010.03400.x			13	Plant Sciences	Plant Sciences	646OI	WOS:000281551500013	20862781	Bronze			2021-04-07	
J	Bothwell, JH; Marie, D; Peters, AF; Cock, JM; Coelho, SM				Bothwell, John H.; Marie, Dominique; Peters, Akira F.; Cock, J. Mark; Coelho, Susana M.			Role of endoreduplication and apomeiosis during parthenogenetic reproduction in the model brown alga Ectocarpus	NEW PHYTOLOGIST			English	Article						apomeiosis; cell cycle; Ectocarpus; endoreduplication; haploid-diploid; life cycle	GENOME-WIDE ANALYSIS; CELL-CYCLE GENES; SILICULOSUS ECTOCARPALES; MOLECULAR PHYLOGENY; LIFE-HISTORY; PHAEOPHYCEAE; PLANTS; CULTURE; ARABIDOPSIS; ANNOTATION	P>The filamentous brown alga Ectocarpus has a complex life cycle, involving alternation between independent and morphologically distinct sporophyte and gametophyte generations. In addition to this basic haploid-diploid life cycle, gametes can germinate parthenogenetically to produce parthenosporophytes. This article addresses the question of how parthenosporophytes, which are derived from a haploid progenitor cell, are able to produce meiospores in unilocular sporangia, a process that normally involves a reductive meiotic division. We used flow cytometry, multiphoton imaging, culture studies and a bioinformatics survey of the recently sequenced Ectocarpus genome to describe its life cycle under laboratory conditions and the nuclear DNA changes which accompany key developmental transitions. Endoreduplication occurs during the first cell cycle in about one-third of parthenosporophytes. The production of meiospores by these diploid parthenosporophytes involves a meiotic division similar to that observed in zygote-derived sporophytes. By contrast, meiospore production in parthenosporophytes that fail to endoreduplicate occurs via a nonreductive apomeiotic event. Our results highlight Ectocarpus's reproductive and developmental plasticity and are consistent with previous work showing that its life cycle transitions are controlled by genetic mechanisms and are independent of ploidy.	[Bothwell, John H.; Cock, J. Mark; Coelho, Susana M.] CNRS, UMR 7139, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, F-29682 Roscoff, France; [Bothwell, John H.] Queens Univ, Marine Lab, Portaferry BT22 1PF, North Ireland; [Bothwell, John H.] Queens Univ Belfast, Sch Biol Sci, Belfast BT9 7BL, Antrim, North Ireland; [Bothwell, John H.; Cock, J. Mark; Coelho, Susana M.] Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, F-29682 Roscoff, France; [Bothwell, John H.] Marine Biol Assoc United Kingdom Lab, Plymouth PL1 2PB, Devon, England; [Marie, Dominique] UPMC, CNRS, UMR 7144, Divers Ocean Plankton Grp,Stn Biol Roscoff, F-29682 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29680 Roscoff, France	Coelho, SM (corresponding author), CNRS, UMR 7139, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, Pl Georges Teissier,BP74, F-29682 Roscoff, France.	coelho@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020	Cock, J. Mark/0000-0002-2650-0383; Peters, Akira/0000-0001-5332-199X	Leverhulme TrustLeverhulme Trust; Centre National de la Recherche ScientifiqueCentre National de la Recherche Scientifique (CNRS); University Pierre and Marie Curie; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [MBA010001] Funding Source: researchfish	We thank Genoscope for access to the assembled Ectocarpus genome, the Bioinformatics group at the Universiteit Gent for automatic gene predictions, Herve Moreau of the Oceanological Observatory in Banyuls for help in annotating cell cycle genes, and Laurence Dartevelle for assistance with the cultures of Ectocarpus. J.H.B. was funded by a Leverhulme Trust Early Career Fellowship. This work was supported by the Centre National de la Recherche Scientifique and the University Pierre and Marie Curie. Finally, the manuscript was greatly improved by the critical reading of three anonymous referees, to whom we are extremely grateful.	Aylon Y, 2004, DNA REPAIR, V3, P797, DOI 10.1016/j.dnarep.2004.04.013; Barow M, 2003, PLANT CELL ENVIRON, V26, P571, DOI 10.1046/j.1365-3040.2003.00988.x; Bisova K, 2005, PLANT PHYSIOL, V137, P475, DOI 10.1104/pp.104.054155; Bothwell JHF, 2008, DEVELOPMENT, V135, P2173, DOI 10.1242/dev.017558; Cho GY, 2004, J PHYCOL, V40, P921, DOI 10.1111/j.1529-8817.2004.03160.x; Clamp M, 2004, BIOINFORMATICS, V20, P426, DOI 10.1093/bioinformatics/btg430; Cock JM, 2010, NATURE, V465, P617, DOI 10.1038/nature09016; DEREVIERS B, 2003, BIOL PHYLOGENIE ALGU, V2; DESHMUKHE GV, 1993, PHYCOLOGIA, V32, P197, DOI 10.2216/i0031-8884-32-3-197.1; Eck R. V., 1966, ATLAS PROTEIN SEQUEN; Edgar BA, 2001, CELL, V105, P297, DOI 10.1016/S0092-8674(01)00334-8; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; Foissac S, 2008, CURR BIOINFORM, V3, P87, DOI 10.2174/157489308784340702; Garbary DJ, 2002, BOT MAR, V45, P211, DOI 10.1515/BOT.2002.020; Garbary DJ, 1998, MOL APPROACHES STUDY, P409; Gendreau E, 1997, PLANT PHYSIOL, V114, P295, DOI 10.1104/pp.114.1.295; GOFF L. J., 1990, BIOL RED ALGAE, P43; Helmchen F, 2005, NAT METHODS, V2, P932, DOI 10.1038/NMETH818; Huysman MJJ, 2010, GENOME BIOL, V11, DOI 10.1186/gb-2010-11-2-r17; Inze D, 2006, ANNU REV GENET, V40, P77, DOI 10.1146/annurev.genet.40.110405.090431; JENSEN J B, 1974, University of California Publications in Botany, V68, P1; John PCL, 2008, TRENDS PLANT SCI, V13, P121, DOI 10.1016/j.tplants.2008.01.004; Kawai H, 2007, J PHYCOL, V43, P186, DOI 10.1111/j.1529-8817.2006.00308.x; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; KORNMANN P., 1956, PUBBL STAZ ZOOL NAPOLI, V28, P32; KUHLENKAMP R, 1985, BRIT PHYCOL J, V20, P301, DOI 10.1080/00071618500650311; Le Bail A, 2008, J PHYCOL, V44, P1269, DOI 10.1111/j.1529-8817.2008.00582.x; LEE YK, 1995, J PHYCOL, V31, P668, DOI 10.1111/j.1529-8817.1995.tb02564.x; LEGALL E, 1996, EUR J PHYCOL, V31, P369; Meijer M, 2001, CURR OPIN PLANT BIOL, V4, P44, DOI 10.1016/S1369-5266(00)00134-5; Muller D. G., 1963, PUBBLICAZIONI STAZIO, V33, P310; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1986, HELGOLANDER MEERESUN, V40, P219; MULLER DG, 1975, ARCH PROTISTENKD, V117, P297; NAGL W, 1976, NATURE, V261, P614, DOI 10.1038/261614a0; PAPENFUSS GEORGE F., 1935, BOT GAZ, V96, P421, DOI 10.1086/334493; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Phillips N, 2008, J PHYCOL, V44, P15, DOI 10.1111/j.1529-8817.2007.00435.x; Robbens S, 2005, MOL BIOL EVOL, V22, P589, DOI 10.1093/molbev/msi044; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Sugimoto-Shirasu K, 2003, CURR OPIN PLANT BIOL, V6, P544, DOI 10.1016/j.pbi.2003.09.009; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Vandepoele K, 2002, PLANT CELL, V14, P903, DOI 10.1105/tpc.010445	47	30	30	2	31	WILEY-BLACKWELL	MALDEN	COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA	0028-646X			NEW PHYTOL	New Phytol.		2010	188	1					111	121		10.1111/j.1469-8137.2010.03357.x			11	Plant Sciences	Plant Sciences	646OI	WOS:000281551500014	20618911				2021-04-07	
J	Heesch, S; Cho, GY; Peters, AF; Le Corguille, G; Falentin, C; Boutet, G; Coedel, S; Jubin, C; Samson, G; Corre, E; Coelho, SM; Cock, JM				Heesch, Svenja; Cho, Ga Youn; Peters, Akira F.; Le Corguille, Gildas; Falentin, Cyril; Boutet, Gilles; Coedel, Solene; Jubin, Claire; Samson, Gaelle; Corre, Erwan; Coelho, Susana M.; Cock, J. Mark			A sequence-tagged genetic map for the brown alga Ectocarpus siliculosus provides large-scale assembly of the genome sequence	NEW PHYTOLOGIST			English	Article						chromosomes; Ectocarpus siliculosus; genetic linkage map; microsatellites; model organism; Phaeophyceae	PHYTOPHTHORA-INFESTANS; AVIRULENCE GENES; SEXUAL PROGENY; LINKAGE MAP; PHAEOPHYCEAE; INHERITANCE; OOMYCETE; MARKERS; AFLP; PCR	Ectocarpus siliculosus has been proposed as a genetic and genomic model for the brown algae and the 214 Mbp genome of this organism has been sequenced. The aim of this project was to obtain a chromosome-scale view of the genome by constructing a genetic map using microsatellite markers that were designed based on the sequence supercontigs. To map genetic markers, a segregating F(2) population was generated from a cross between the sequenced strain (Ec 32) and a compatible strain from northern Chile. Amplified fragment length polymorphism (AFLP) analysis indicated a significant degree of polymorphism (41%) between the genomes of these two parental strains. Of 1,152 microsatellite markers that were selected for analysis based on their location on long supercontigs, their potential as markers and their predicted ability to amplify a single genomic locus, 407 were found to be polymorphic. A genetic map was constructed using 406 markers, resulting in 34 linkage groups. The 406 markers anchor 325 of the longest supercontigs on to the map, representing 70.1% of the genome sequence. The Ectocarpus genetic map described here not only provides a large-scale assembly of the genome sequence, but also represents an important tool for future genetic analysis using this organism.	[Heesch, Svenja; Cho, Ga Youn; Coelho, Susana M.; Cock, J. Mark] Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, F-29682 Roscoff, France; [Heesch, Svenja; Cho, Ga Youn; Coelho, Susana M.; Cock, J. Mark] CNRS, UMR 7139, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, F-29682 Roscoff, France; [Peters, Akira F.] Bezhin Rosko, F-29680 Roscoff, France; [Le Corguille, Gildas; Corre, Erwan] Anal & Bioinformat Marine Sci ABiMS Platform Comp, FR 2424, Stn Biol Roscoff, F-29682 Roscoff, France; [Falentin, Cyril] Univ Rennes 1, INRA, UMR APBV Plant Genet & Biotechnol 118, F-35653 Le Rheu, France; [Boutet, Gilles; Coedel, Solene] Univ Rennes 1, INRA, UMR 118, F-35653 Le Rheu, France; [Jubin, Claire; Samson, Gaelle] CEA, DSV, Inst Genom, F-91057 Evry, France	Cock, JM (corresponding author), Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, Pl Georges Teissier,BP74, F-29682 Roscoff, France.	cock@sb-roscoff.fr	Coelho, Susana/ABH-8166-2020; corre, erwan/O-4669-2019	corre, erwan/0000-0001-6354-2278; Le Corguille, Gildas/0000-0003-1742-9711; Peters, Akira/0000-0001-5332-199X; Heesch, Svenja/0000-0002-4531-0921; Cock, J. Mark/0000-0002-2650-0383	Conseil Generale de Finistere; Korean Research FoundationKorea Research Foundation; GIS Europole Mer; Centre National de Recherche Scientifique; European Union network of excellence Marine Genomics Europe; Europole Mer and the University Pierre and Marie Curie	This project was supported by grants to S.H. from the Conseil Generale de Finistere, and to G.Y.C. from the Korean Research Foundation, and by funding from the GIS Europole Mer, the Centre National de Recherche Scientifique, the European Union network of excellence Marine Genomics Europe, the Europole Mer and the University Pierre and Marie Curie. We would also like to acknowledge the help of Delphine Scornet, Sylvie Rousvoal, Laurence Dartevelle, Sarah Stewart, Morgan Perennou, Sarah Romac, Serinde J. van Wijk (all at the Station Biologique de Roscoff, France; Serinde J. van Wijk is now at Bangor University, UK), and Gwenn Verplancke (INRA, Le Rheu, France).	Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Carter DA, 1999, FUNGAL GENET BIOL, V26, P198, DOI 10.1006/fgbi.1999.1120; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; COCK JM, NATURE IN PRESS; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Dittami SM, 2009, GENOME BIOL, V10, DOI 10.1186/gb-2009-10-6-r66; Dobrowolski MP, 2002, FUNGAL GENET BIOL, V35, P197, DOI 10.1006/fgbi.2001.1319; Jewell E, 2006, NUCLEIC ACIDS RES, V34, pW656, DOI 10.1093/nar/gkl083; Judelson HS, 1999, PHYTOPATHOLOGY, V89, P754, DOI 10.1094/PHYTO.1999.89.9.754; Judelson HS, 1996, GENETICS, V144, P1005; Klarzynski O, 2000, PLANT PHYSIOL, V124, P1027, DOI 10.1104/pp.124.3.1027; KNIGHT M, 1931, P ROY SOC EDINB, V56, P47; LANDER E S, 1987, Genomics, V1, P174, DOI 10.1016/0888-7543(87)90010-3; May KJ, 2002, FUNGAL GENET BIOL, V37, P1, DOI 10.1016/S1087-1845(02)00027-0; MULLER DG, 1966, PLANTA, V68, P57, DOI 10.1007/BF00385371; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; PETERS AF, 2010, NEW PHYTOLO IN PRESS; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Schuelke M, 2000, NAT BIOTECHNOL, V18, P233, DOI 10.1038/72708; Schuler GD, 1997, GENOME RES, V7, P541, DOI 10.1101/gr.7.5.541; Sicard D, 2003, FUNGAL GENET BIOL, V39, P16, DOI 10.1016/S1087-1845(03)00005-7; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; van der Lee T, 2004, GENETICS, V167, P1643, DOI 10.1534/genetics.104.029652; van der Lee T, 2001, MOL PLANT MICROBE IN, V14, P1444, DOI 10.1094/MPMI.2001.14.12.1444; VanderLee T, 1997, FUNGAL GENET BIOL, V21, P278, DOI 10.1006/fgbi.1997.0981; VOS P, 1995, NUCLEIC ACIDS RES, V23, P4407, DOI 10.1093/nar/23.21.4407; Yang GP, 2009, J PHYCOL, V45, P873, DOI 10.1111/j.1529-8817.2009.00720.x	30	48	48	0	29	WILEY-BLACKWELL	MALDEN	COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA	0028-646X			NEW PHYTOL	New Phytol.		2010	188	1								10.1111/j.1469-8137.2010.03273.x			10	Plant Sciences	Plant Sciences	646OI	WOS:000281551500002	20456050	Bronze			2021-04-07	
J	Le Corguille, G; Pearson, G; Valente, M; Viegas, C; Gschloessl, B; Corre, E; Bailly, X; Peters, AF; Jubin, C; Vacherie, B; Cock, JM; Leblanc, C				Le Corguille, Gildas; Pearson, Gareth; Valente, Marta; Viegas, Carla; Gschloessl, Bernhard; Corre, Erwan; Bailly, Xavier; Peters, Akira F.; Jubin, Claire; Vacherie, Benoit; Cock, J. Mark; Leblanc, Catherine			Plastid genomes of two brown algae, Ectocarpus siliculosus and Fucus vesiculosus: further insights on the evolution of red-algal derived plastids	BMC EVOLUTIONARY BIOLOGY			English	Article							HORIZONTAL GENE-TRANSFER; CHLOROPLAST GENOME; CHROMALVEOLATE PLASTIDS; CHLOROPHYLL-C; PHOTOSYNTHETIC EUKARYOTES; MULTIPLE ENDOSYMBIOSES; MAXIMUM-LIKELIHOOD; COMPLETE SEQUENCE; CRYPTOPHYTE ALGA; COMMON ANCESTRY	Background: Heterokont algae, together with cryptophytes, haptophytes and some alveolates, possess red-algal derived plastids. The chromalveolate hypothesis proposes that the red-algal derived plastids of all four groups have a monophyletic origin resulting from a single secondary endosymbiotic event. However, due to incongruence between nuclear and plastid phylogenies, this controversial hypothesis remains under debate. Large-scale genomic analyses have shown to be a powerful tool for phylogenetic reconstruction but insufficient sequence data have been available for red-algal derived plastid genomes. Results: The chloroplast genomes of two brown algae, Ectocarpus siliculosus and Fucus vesiculosus, have been fully sequenced. These species represent two distinct orders of the Phaeophyceae, which is a major group within the heterokont lineage. The sizes of the circular plastid genomes are 139,954 and 124,986 base pairs, respectively, the size difference being due principally to the presence of longer inverted repeat and intergenic regions in E. siliculosus. Gene contents of the two plastids are similar with 139-148 protein-coding genes, 28-31 tRNA genes, and 3 ribosomal RNA genes. The two genomes also exhibit very similar rearrangements compared to other sequenced plastid genomes. The tRNA-Leu gene of E. siliculosus lacks an intron, in contrast to the F. vesiculosus and other heterokont plastid homologues, suggesting its recent loss in the Ectocarpales. Most of the brown algal plastid genes are shared with other red-algal derived plastid genomes, but a few are absent from raphidophyte or diatom plastid genomes. One of these regions is most similar to an apicomplexan nuclear sequence. The phylogenetic relationship between heterokonts, cryptophytes and haptophytes ( collectively referred to as chromists) plastids was investigated using several datasets of concatenated proteins from two cyanobacterial genomes and 18 plastid genomes, including most of the available red algal and chromist plastid genomes. Conclusion: The phylogenetic studies using concatenated plastid proteins still do not resolve the question of the monophyly of all chromist plastids. However, these results support both the monophyly of heterokont plastids and that of cryptophyte and haptophyte plastids, in agreement with nuclear phylogenies.	[Gschloessl, Bernhard; Peters, Akira F.; Cock, J. Mark; Leblanc, Catherine] CNRS, Biol Stn, UMR7139, Roscoff, France; [Le Corguille, Gildas; Corre, Erwan; Bailly, Xavier] CNRS, Biol Stn, FR2424, Comp & Genom Resource Ctr, Roscoff, France; [Le Corguille, Gildas; Corre, Erwan; Bailly, Xavier] Univ Paris 06, FR2424, Comp & Genom Resource Ctr, Biol Stn, Roscoff, France; [Pearson, Gareth; Valente, Marta; Viegas, Carla] Univ Algarve, Ctr Marine Sci, Faro, Portugal; [Gschloessl, Bernhard; Peters, Akira F.; Cock, J. Mark; Leblanc, Catherine] Univ Paris 06, UMR7139, Biol Stn, Roscoff, France; [Jubin, Claire; Vacherie, Benoit] CEA, DSV, Inst Genom, Evry, France; [Jubin, Claire] CNRS, UMR 8030, Evry, France; [Jubin, Claire] Univ Evry, Evry, France	Leblanc, C (corresponding author), CNRS, Biol Stn, UMR7139, Roscoff, France.	lecorguille@sb-roscoff.fr; gpearson@ualg.pt; msvalente@ualg.pt; caviegas@ualg.pt; bernhard.gschloessl@univ-rennes1.fr; corre@sb-roscoff.fr; bailly@sb-roscoff.fr; akirapeters@gmail.com; claire.jubin@gmail.com; bvacheri@genoscope.cns.fr; cock@sb-roscoff.fr; leblanc@sb-roscoff.fr	Pearson, Gareth/J-3911-2013; corre, erwan/O-4669-2019; Viegas, Carla/N-6314-2019; Viegas, Carla CSB/N-6695-2014	Pearson, Gareth/0000-0002-0768-464X; corre, erwan/0000-0001-6354-2278; Viegas, Carla/0000-0002-5765-3665; Viegas, Carla CSB/0000-0002-5765-3665; Cock, J. Mark/0000-0002-2650-0383; Peters, Akira/0000-0001-5332-199X; Le Corguille, Gildas/0000-0003-1742-9711	Brittany Regional CouncilRegion Bretagne; FCT-FEDER (Portugal)Portuguese Foundation for Science and Technology	We are grateful to Alexander Goesmann and Virginie Mittard-Runte for providing access to the GenDB platform and to Hameed Khan and John M. Archibald for providing their 45 concatenated-protein alignment. We also thank Nicolas Lartillot for making available the last version of Phylobayes 3.1d. This work, performed within the framework of Marine Genomics Europe NoE 7 (EC contract N GOCE-CT-2004-505403), was partially supported by the Brittany Regional Council ( G. L. C. grant) and by FCT-FEDER (Portugal).	Adachi J, 2000, J MOL EVOL, V50, P348, DOI 10.1007/s002399910038; Adl SM, 2005, J EUKARYOT MICROBIOL, V52, P399, DOI 10.1111/j.1550-7408.2005.00053.x; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Archibald JM, 2009, CURR BIOL, V19, pR81, DOI 10.1016/j.cub.2008.11.067; Bachvaroff TR, 2005, MOL BIOL EVOL, V22, P1772, DOI 10.1093/molbev/msi172; Baldauf SL, 2000, SCIENCE, V290, P972, DOI 10.1126/science.290.5493.972; Bhattacharya D, 2008, J PHYCOL, V44, P7, DOI 10.1111/j.1529-8817.2007.00433.x; Bhattacharya D, 2007, BIOESSAYS, V29, P1239, DOI 10.1002/bies.20671; Bodyl A, 2009, TRENDS ECOL EVOL, V24, P119, DOI 10.1016/j.tree.2008.11.003; Bowler C, 2008, NATURE, V456, P239, DOI 10.1038/nature07410; Braun EL, 2008, J PHYCOL, V44, P2, DOI 10.1111/j.1529-8817.2007.00432.x; Burki F, 2008, BIOL LETTERS, V4, P366, DOI 10.1098/rsbl.2008.0224; Burki F, 2007, PLOS ONE, V2, DOI 10.1371/journal.pone.0000790; Castresana J, 2000, MOL BIOL EVOL, V17, P540, DOI 10.1093/oxfordjournals.molbev.a026334; Cattolico RA, 2008, BMC GENOMICS, V9, DOI 10.1186/1471-2164-9-211; Cavalier-Smith T, 1998, BIOL REV, V73, P203, DOI 10.1017/S0006323198005167; Cavalier-Smith T, 1999, J EUKARYOT MICROBIOL, V46, P347, DOI 10.1111/j.1550-7408.1999.tb04614.x; Douglas SE, 1999, J MOL EVOL, V48, P236, DOI 10.1007/PL00006462; DOYLE JJ, 1988, AM J BOT, V75, P1238, DOI 10.2307/2444108; Dyall SD, 2004, SCIENCE, V304, P253, DOI 10.1126/science.1094884; Edgar RC, 2004, NUCLEIC ACIDS RES, V32, P1792, DOI 10.1093/nar/gkh340; Felsenstein J., 1989, CLADISTICS, V5, P164, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X; Frommolt R, 2008, MOL BIOL EVOL, V25, P2653, DOI 10.1093/molbev/msn206; Glockner G, 2000, J MOL EVOL, V51, P382; Gould SB, 2008, ANNU REV PLANT BIOL, V59, P491, DOI 10.1146/annurev.arplant.59.032607.092915; Gross J, 2009, TRENDS PLANT SCI, V14, P13, DOI 10.1016/j.tplants.2008.10.003; Guindon S, 2003, SYST BIOL, V52, P696, DOI 10.1080/10635150390235520; Hackett JD, 2007, MOL BIOL EVOL, V24, P1702, DOI 10.1093/molbev/msm089; Hagopian JC, 2004, J MOL EVOL, V59, P464, DOI 10.1007/s00239-004-2638-3; Hampl V, 2009, P NATL ACAD SCI USA, V106, P3859, DOI 10.1073/pnas.0807880106; Harper JT, 2003, MOL BIOL EVOL, V20, P1730, DOI 10.1093/molbev/msg195; Huang JL, 2008, GENOME BIOL, V9, DOI 10.1186/gb-2008-9-7-r109; Iida K, 2007, MOL PHYLOGENET EVOL, V45, P227, DOI 10.1016/j.ympev.2007.05.003; JONES DT, 1992, COMPUT APPL BIOSCI, V8, P275, DOI 10.1093/bioinformatics/8.3.275; Kai A, 2008, PROTIST, V159, P435, DOI 10.1016/j.protis.2007.12.003; Kalanon M, 2008, GENETICS, V179, P95, DOI 10.1534/genetics.107.085704; Keeling PJ, 2009, J EUKARYOT MICROBIOL, V56, P1, DOI 10.1111/j.1550-7408.2008.00371.x; Keeling PJ, 2004, AM J BOT, V91, P1481, DOI 10.3732/ajb.91.10.1481; Khan H, 2007, MOL BIOL EVOL, V24, P1832, DOI 10.1093/molbev/msm101; Koumandou VL, 2004, TRENDS GENET, V20, P261, DOI 10.1016/j.tig.2004.03.008; Kowallik KV, 1995, PLANT MOL BIOL REP, V13, P336, DOI 10.1007/BF02669188; Lagesen K, 2007, NUCLEIC ACIDS RES, V35, P3100, DOI 10.1093/nar/gkm160; Lane CE, 2009, TRENDS ECOL EVOL, V24, P121, DOI 10.1016/j.tree.2008.11.002; Lartillot N, 2004, MOL BIOL EVOL, V21, P1095, DOI 10.1093/molbev/msh112; Lartillot N, 2007, BMC EVOL BIOL, V7, DOI 10.1186/1471-2148-7-S1-S4; Laslett D, 2004, NUCLEIC ACIDS RES, V32, P11, DOI 10.1093/nar/gkh152; Li SL, 2006, MOL BIOL EVOL, V23, P663, DOI 10.1093/molbev/msj075; Meyer F, 2003, NUCLEIC ACIDS RES, V31, P2187, DOI 10.1093/nar/gkg312; Moore RB, 2008, NATURE, V451, P959, DOI 10.1038/nature06635; Moustafa A, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0002205; Not F, 2007, SCIENCE, V315, P253, DOI 10.1126/science.1136264; Ohta N, 2003, DNA RES, V10, P67, DOI 10.1093/dnares/10.2.67; Oudot-Le Secq MP, 2007, MOL GENET GENOMICS, V277, P427, DOI 10.1007/s00438-006-0199-4; Parfrey LW, 2006, PLOS GENET, V2, P2062, DOI 10.1371/journal.pgen.0020220; Patron NJ, 2007, CURR BIOL, V17, P887, DOI 10.1016/j.cub.2007.03.069; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Phillips N, 2008, J PHYCOL, V44, P394, DOI 10.1111/j.1529-8817.2008.00473.x; Puerta MVS, 2005, DNA RES, V12, P151, DOI 10.1093/dnares/12.2.151; Reith M, 1995, PLANT MOL BIOL REP, V13, P333, DOI 10.1007/BF02669187; Reyes-Prieto A, 2008, CURR BIOL, V18, P956, DOI 10.1016/j.cub.2008.05.042; Reyes-Prieto A, 2007, ANNU REV GENET, V41, P147, DOI 10.1146/annurev.genet.41.110306.130134; Rice DW, 2006, BMC BIOL, V4, DOI 10.1186/1741-7007-4-31; Riisberg I, 2009, PROTIST, V160, P191, DOI 10.1016/j.protis.2008.11.004; Rodriguez-Ezpeleta N, 2007, CURR BIOL, V17, P1420, DOI 10.1016/j.cub.2007.07.036; Rodriguez-Ezpeleta N, 2007, MOL BIOL EVOL, V24, P723, DOI 10.1093/molbev/msl200; Rogers MB, 2007, MOL BIOL EVOL, V24, P54, DOI 10.1093/molbev/msl129; Rokas A, 2005, MOL BIOL EVOL, V22, P1337, DOI 10.1093/molbev/msi121; Rumpho ME, 2008, P NATL ACAD SCI USA, V105, P17867, DOI 10.1073/pnas.0804968105; Sanchez-Puerta MV, 2008, J PHYCOL, V44, P1097, DOI 10.1111/j.1529-8817.2008.00559.x; Sanchez-Puerta MV, 2007, MOL PHYLOGENET EVOL, V44, P885, DOI 10.1016/j.ympev.2007.03.003; Shimodaira H, 2001, BIOINFORMATICS, V17, P1246, DOI 10.1093/bioinformatics/17.12.1246; Simon D, 2003, J MOL EVOL, V57, P710, DOI 10.1007/s00239-003-2533-3; Slamovits CH, 2008, MOL BIOL EVOL, V25, P1297, DOI 10.1093/molbev/msn075; Strimmer K, 1996, MOL BIOL EVOL, V13, P964, DOI 10.1093/oxfordjournals.molbev.a025664; van Dooren GG, 2008, P NATL ACAD SCI USA, V105, P13574, DOI 10.1073/pnas.0803862105; Walter RF, 2005, CURR ISSUES MOL BIOL, V7, P57; Wang YL, 2008, J MOL EVOL, V66, P175, DOI 10.1007/s00239-008-9070-z; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075; Yoon HS, 2002, P NATL ACAD SCI USA, V99, P15507, DOI 10.1073/pnas.242379899; Yoon HS, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-14; Zhang ZD, 1999, NATURE, V400, P155	82	65	67	1	18	BIOMED CENTRAL LTD	LONDON	236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND	1471-2148			BMC EVOL BIOL	BMC Evol. Biol.	OCT 16	2009	9								253	10.1186/1471-2148-9-253			14	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	511RQ	WOS:000271184700001	19835607	DOAJ Gold, Green Published			2021-04-07	
J	Aisha, K; Shameel, M				Aisha, K.; Shameel, Mustafa			OCCURRENCE OF THE GENUS BACHELOTIA (ECTOCARPALES, PHAEOPHYCOTA) IN THE COASTAL WATERS OF PAKISTAN	PAKISTAN JOURNAL OF BOTANY			English	Article							KARACHI COAST; ALGAE	A rarely occurring brown alga, Bachelotia antillarum (Grunow) Gerloff [=Ectocarpus antillarum Grunow] was collected from the coastal areas near Karachi, Pakistan and taxonomically investigated. This is the first report of occurrence of the genus Bachelotia (Bornet) Kuckuck ex G. Hamel from northern Arabian Sea, It is Suggested to place it in the family Pilayellaccae Pedersen, as its thallus becomes parenchymatous due to the formation of a few longitudinal divisions and as its sporangia arise by a simple transformation of vegetative cells.	[Aisha, K.; Shameel, Mustafa] Univ Karachi, Dept Bot, Karachi 75270, Pakistan	Aisha, K (corresponding author), Univ Karachi, Dept Bot, Karachi 75270, Pakistan.						BEGUM M, 1992, PAK J BOT, V24, P22; BEGUM M, 1992, BOT J LINN SOC, V108, P239, DOI 10.1111/j.1095-8339.1992.tb00241.x; BOrgesen F., 1920, DANSK BOT ARK, V3, P369; Earle SA, 1969, PHYCOLOGIA, V7, P71, DOI 10.2216/i0031-8884-7-2-71.1; HAMEL G., 1937, PHEOPHYCEES FRANCE, P177; Krishnamurthy V., 1970, CHECK LIST INDIAN MA; LINDAUER VW, 1961, NOVA HEDWIGIA, V3, P129; PEDERSEN P.M., 1984, OPERA BOT, V74, P1; SALIM KM, 1965, BOT MAR, V8, P183, DOI 10.1515/botm.1965.8.2-4.183; Shaikh W., 1995, PAK J MAR SCI, V4, P9; Shameel M, 1996, BOT MAR, V39, P223, DOI 10.1515/botm.1996.39.1-6.223; Shameel M., 2000, P NAT O N R S AR SEA, P45; Shameel M., 2001, PAK J MAR BIOL, V7, P233; Shameel M., 1992, CRYPTOGAMIC FLORA PA, V1, P1; Shameel Mustafa, 2008, International Journal of Phycology and Phycochemistry, V4, P225; Silva P.C., 1996, CATALOGUE BENTHIC MA; Womersely H.B.S, 1987, MARINE BENTHIC FLO 2	17	0	2	0	0	PAKISTAN BOTANICAL SOC	KARACHI	DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN	0556-3321			PAK J BOT	Pak. J. Bot.	AUG	2009	41	4					1917	1920					4	Plant Sciences	Plant Sciences	511TQ	WOS:000271192300044					2021-04-07	
J	Racault, MFLP; Fletcher, RL; de Reviers, B; Cho, GY; Boo, SM; Parente, MI; Rousseau, F				Racault, Marie-Fanny L. P.; Fletcher, Robert L.; de Reviers, Bruno; Cho, Ga Youn; Boo, Sung Min; Parente, Manuela I.; Rousseau, Florence			Molecular phylogeny of the brown algal genus Petrospongium Nageli ex Kutz. (Phaeophyceae) with evidence for Petrospongiaceae fam. nov.	CRYPTOGAMIE ALGOLOGIE			English	Article						Corynophlaea; Ectocarpales; molecular phylogeny; Petrospongiaceae; Petrospongium; Phaeophyceae; psaA; rbcL	SYSTEMATIC POSITION; ECTOCARPALES; SEQUENCES; ORDER; MODEL; RBCL; CIRCUMSCRIPTION; ADENOCYSTACEAE; SUBSTITUTION; CHECKLIST	Based on morphological evidence, the systematic position of the genus Petrospongium and its relationship with the genera Leathesia and Corynophlaea have long been disputed. In the present study, the position within the order Ectocarpales of the type species, Petrospongium berkeleyi (Grev. in Berk.) Nageli ex Kutz., has been investigated using molecular phylogenetic analysis based on rbcL and psaA sequences. Petrospongium berkeleyi and its Pacific vicariant P. rugosum appeared closely related and did not cluster with other members of the Chordariaceac, particularly the genera Leathesia and Corynophlaea, nor with any other currently recognised ectocarpalean family. The genus Petrospongium formed an independent monophyletic group which was a sister clade of the family Ectocarpaceae. The genera Ectocarpus and Petrospongium differ in their mode of thallus construction (filamentous thallus and pseudoparenchymatous cushion-like thallus, respectively), and in the type of plastids (ribbon-shape and discoid, respectively). These morphological differences agree with our phylogenetic analyses and lead us to propose the family Petrospongiaceae fam. nov. to accommodate the genus Petrospongium.	[Racault, Marie-Fanny L. P.; Fletcher, Robert L.] Univ Portsmouth, Sch Biol Sci, Inst Marine Sci, Portsmouth PO4 9LY, Hants, England; [de Reviers, Bruno; Rousseau, Florence] UPMC, UMR, MNHN,CNRS,IRD SAE 7138, Museum Natl Hist Nat,Dept Sytemat & Evolut, F-75231 Paris 05, France; [Cho, Ga Youn] Natl Inst Biol Resources, Nonvasc Plant Res Div, Inchon 404708, South Korea; [Boo, Sung Min] Chungnam Natl Univ, Dept Biol, Taejon 305764, South Korea; [Parente, Manuela I.] Univ Nova Lisboa, Fac Ciencias & Tecnol, IMAR, Dept Ciencias & Engn Ambiente, P-2829516 Caparica, Portugal; [Parente, Manuela I.] Univ Acores, Dept Biol, P-9501855 Ponta Delgada, Portugal	Racault, MFLP (corresponding author), Univ Portsmouth, Sch Biol Sci, Inst Marine Sci, Ferry Rd, Portsmouth PO4 9LY, Hants, England.	m.racault@uea.ac.uk	Racault, Marie-Fanny LP/D-7190-2016; Parente, Manuela/AAE-1346-2020	Racault, Marie-Fanny LP/0000-0002-7584-2515; Parente, Manuela/0000-0003-0204-7155; Fletcher, Robert/0000-0002-1106-7863			Abbott I. A., 1976, MARINE ALGAE CALIFOR; AKAIKE H, 1974, IEEE T AUTOMAT CONTR, VAC19, P716, DOI 10.1109/TAC.1974.1100705; ASSALI NE, 1990, PLANT MOL BIOL, V15, P307, DOI 10.1007/BF00036916; CARAM B, 1957, CR HEBD ACAD SCI, V245, P440; Cho GY, 2004, J PHYCOL, V40, P921, DOI 10.1111/j.1529-8817.2004.03160.x; Cho GY, 2006, CRYPTOGAMIE ALGOL, V27, P3; Cho TO, 2003, NOVA HEDWIGIA, V76, P381, DOI 10.1127/0029-5035/2003/0076-0381; Christensen T, 1994, ALGAE TAXONOMIC SURV; CROUAN P.L., 1851, ANN SCI NAT BOT 3, V15, P359; Draisma S. G. A., 2003, OUT PAST COLLECTED R, P87; FARRIS JS, 1994, CLADISTICS, V10, P315, DOI 10.1111/j.1096-0031.1996.tb00196.x; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; FLETCHER R.L., 1987, SEAWEEDS BRIT ISLES, V3; GAYRAL P, 1966, ALGAES COTES FRANCAI; Giaccone G, 1978, ANN WORLD WILDLIFE S, V6, P1; Gray S. F., 1821, NATURAL ARRANGEMENT, V1; HAMEL G, 1939, PHEOPHYCEES FRANCE, P337; HAMEL G., 1937, PHEOPHYCEES FRANCE, P177; HAMEL G., 1935, PHEOPHYCEES FRANCE, P81; HAMEL G, 1938, PHEOPHYCEES FRANCE, P241; Hamel G, 1931, PHEOPHYCEES FRANCE, P1; HANNA H, 1899, ANN BOT, V13, P461; Haroun RJ, 2002, BOT MAR, V45, P139, DOI 10.1515/BOT.2002.015; HARVEY W. H., 1849, PHYCOLOGIA BRITANNIC, VII; HARVEY WH, 1846, PHYCOLOGIA BRITANNIC, V1; HARVEY WH, 1851, PHYCOLOGIA BRITANNIC, V3; HORI T, 1993, ILLUSTRATED ATLAS LI, V2; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; Kawai H, 2000, PHYCOLOGIA, V39, P416, DOI 10.2216/i0031-8884-39-5-416.1; Kogame K, 1999, PHYCOLOGIA, V38, P496, DOI 10.2216/i0031-8884-38-6-496.1; KUCKUCK P., 1929, HELGOLANDER WISSENSC, V17, P1; Kuntze O., 1898, REVISIO GENERUM PL 3; Kutzing F. T., 1843, LINNAEA, V17, P75; KUTZING FT, 1958, TABULAE PHYCOLOGICAE, V8; LONGO MC, 1990, GENE, V93, P125, DOI 10.1016/0378-1119(90)90145-H; Muller DG, 1998, PHYCOLOGIA, V37, P425, DOI 10.2216/i0031-8884-37-6-425.1; Neto A.I., 1994, LIFE MARINE SCI A, V12, P15; Newton L, 1931, HDB BRIT SEAWEEDS; OKAMURA K, 1903, BOT MAG TOKYO, V17, P129; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; RIBERA MA, 1992, BOT MAR, V35, P109, DOI 10.1515/botm.1992.35.2.109; RODRIGUEZ F, 1990, J THEOR BIOL, V142, P485, DOI 10.1016/S0022-5193(05)80104-3; Rousseau F, 1999, CRYPTOGAMIE ALGOL, V20, P5, DOI 10.1016/S0181-1568(99)80002-6; Rousseau F, 2000, EUR J PHYCOL, V35, P35, DOI 10.1017/S0967026200002638; RUSSELL G, 1975, J MAR BIOL ASSOC UK, V55, P763, DOI 10.1017/S0025315400017690; SEGAWA S, 1968, GEN SHOKU NIHON KAIS; Setchell W. A., 1924, P CALIF ACAD SCI, V4, P695; Setchell WA, 1925, U CALIF PUBL BOT, V8, P383; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; SIEMER BL, 1998, THESIS U COPENHAGEN; SMITH GM, 1951, SCIENCE, V113, P457; Stromfelt H. F. G., 1888, NOTARISIA, V3, P381; SWOFFORD DL, 2000, PAUP PHYLOGENETIC AN; Thompson JD, 1994, NUCLEIC ACIDS RES, V22, P425; Uwai S, 2005, EUR J PHYCOL, V40, P179, DOI 10.1080/09670260500128285; Womersley H.B.S., 1987, MARINE BENTHIC FLO 2; Yoon HS, 2002, P NATL ACAD SCI USA, V99, P11724, DOI 10.1073/pnas.172234799; YOSHIDA T, 1990, Japanese Journal of Phycology, V38, P269	59	3	4	0	2	ADAC-CRYPTOGAMIE	PARIS	12 RUE DE BUFFON, 75005 PARIS, FRANCE	0181-1568			CRYPTOGAMIE ALGOL	Cryptogam. Algol.	MAY	2009	30	2					111	123					13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	464LJ	WOS:000267506100002					2021-04-07	
J	Kupper, FC; Gaquerel, E; Cosse, A; Adas, F; Peters, AF; Muller, DG; Kloareg, B; Salaun, JP; Potin, P				Kuepper, Frithjof C.; Gaquerel, Emmanuel; Cosse, Audrey; Adas, Fadi; Peters, Akira F.; Mueller, Dieter G.; Kloareg, Bernard; Salauen, Jean-Pierre; Potin, Philippe			Free Fatty Acids and Methyl Jasmonate Trigger Defense Reactions in Laminaria digitata	PLANT AND CELL PHYSIOLOGY			English	Article						Arachidonic acid; Endophyte; Laminariocolax; Linolenic acid; Methyl jasmonate; Oxidative burst; Polyunsaturated fatty acids; Resistance	CYTOSOLIC PHOSPHOLIPASE A(2); MARINE RED ALGA; OXIDATIVE BURST; ARACHIDONIC-ACID; NADPH OXIDASE; CHONDRUS-CRISPUS; BROWN ALGA; ECTOCARPUS-SILICULOSUS; HYDROGEN-PEROXIDE; DICTYOPTERIS SPP.	Arachidonic acid, linolenic acid and methyl jasmonate (MeJA) were found to be strong triggers of an oxidative burst in the kelp Laminaria digitata. These findings constitute the first report of an oxidative burst in an algal system induced by free fatty acids. The source of reactive oxygen species can be at least partially inhibited by diphenylene iodonium (DPI). Treatment with arachidonic acid increases the levels of a number of free fatty acids [including myristic (C14:0), linoleic (C18:2), linolenic (C18:3) and eicosapentaeneoic (C20:5) acids] and hydroxylated derivatives [such as 15-hydroxyeicosate-traenoic acid (15-HETE), 13-hydroxyoctadecatrienoic acid (13-HOTE) and 15-hydroxyeicosapentaenoic acid (15-HEPE)]. Similar to a previous report of the function of an alginate oligosaccharide-triggered oxidative burst in the establishment of resistance in L. digitata against infection by its brown algal endophyte Laminariocolax tomentosoides, C20:4- and MeJA-induced oxidative bursts seem to be involved in establishing the same protection in L. digitata. Altogether, this study supports the notion that lipid oxidation signaling plays a key role in defense induction in marine brown algae.	[Kuepper, Frithjof C.] Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland; [Kuepper, Frithjof C.; Gaquerel, Emmanuel; Cosse, Audrey; Adas, Fadi; Peters, Akira F.; Kloareg, Bernard; Salauen, Jean-Pierre; Potin, Philippe] Univ Paris 06, CNRS, UMR 7139, Biol Stn, F-29682 Roscoff, Brittany, France; [Kuepper, Frithjof C.; Mueller, Dieter G.] Univ Konstanz, Dept Biol, D-78457 Constance, Germany; [Gaquerel, Emmanuel; Adas, Fadi; Salauen, Jean-Pierre] Univ Bretagne Occidentale, Fac Med, Lab Biochim EA 948, F-29285 Brest, Brittany, France	Kupper, FC (corresponding author), Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland.	fck@sams.ac.uk	Gaquerel, Emmanuel/ABE-6313-2020; Gaquerel, Emmanuel/I-2503-2014; Lenka, Sangram K./A-5830-2009	Gaquerel, Emmanuel/0000-0003-0796-6417; Gaquerel, Emmanuel/0000-0003-0796-6417; Lenka, Sangram K./0000-0002-0121-2430; Peters, Akira/0000-0001-5332-199X; POTIN, Philippe/0000-0001-7358-6282; Kuepper, Frithjof/0000-0003-1273-7109	The Conseil Regional de BretagneRegion Bretagne [560408, A3CBL9]; Studienstiftung des Deutschen Volkes (Bonn); European Commission (Program MAST-III)European CommissionEuropean Commission Joint Research Centre; Natural Environment Research Council (NERC, UK)UK Research & Innovation (UKRI)NERC Natural Environment Research Council; the Ministere de lEducation Nationale, de Ia Recherche et de Ia Technologie and the Max-PlanckGesellschaft; the Institut Francais de Ia Biodiversite; the ECOKELP program [06 BDIV 012]; French Research Agency (ANR)French National Research Agency (ANR); Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [dml010007] Funding Source: researchfish	The Conseil Regional de Bretagne (Programme de Recherche dInteret Regional PRIR-No. 560408; A3CBL9); Studienstiftung des Deutschen Volkes (Bonn) and the European Commission (Program MAST-III) (fellowships to F.C.K. during his graduate studies in Roscoff and Konstanz); the Natural Environment Research Council (NERC, UK) (funding to F.C.K. since taking up his appointment at the Scottish Association for Marine Science); the Ministere de lEducation Nationale, de Ia Recherche et de Ia Technologie and the Max-PlanckGesellschaft (fellowship to E.G.); the Institut Francais de Ia Biodiversite (Programme Biodiversite et Changement Global to P.P.). and the ECOKELP program (06 BDIV 012), funded by the French Research Agency (ANR).	Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Baldridge CW, 1933, AM J PHYSIOL, V103, P235; BHATTACHARYA D, 1991, J MOL EVOL, V33, P525, DOI 10.1007/BF02102805; BLECHERT S, 1995, P NATL ACAD SCI USA, V92, P4099, DOI 10.1073/pnas.92.10.4099; BOSTOCK RM, 1981, SCIENCE, V212, P67, DOI 10.1126/science.212.4490.67; Bouarab K, 2004, PLANT PHYSIOL, V135, P1838, DOI 10.1104/pp.103.037622; Bouarab K, 2001, J APPL PHYCOL, V13, P185, DOI 10.1023/A:1011164031386; Bouarab K, 1999, PLANT CELL, V11, P1635, DOI 10.1105/tpc.11.9.1635; Burkhardt E, 1998, J PHYCOL, V34, P682, DOI 10.1046/j.1529-8817.1998.340682.x; Carpenter LJ, 2000, GLOBAL BIOGEOCHEM CY, V14, P1191, DOI 10.1029/2000GB001257; Chandra S, 1996, PLANT PHYSIOL, V110, P979, DOI 10.1104/pp.110.3.979; COLLEN J, 1994, PHYSIOL PLANTARUM, V92, P417; Collen J, 2006, J EXP BOT, V57, P3869, DOI 10.1093/jxb/erl171; Cronin G, 1996, ECOLOGY, V77, P2287, DOI 10.2307/2265731; Dana R, 1998, J BIOL CHEM, V273, P441, DOI 10.1074/jbc.273.1.441; Dhaunsi GS, 2005, CELL BIOCHEM FUNCT, V23, P65, DOI 10.1002/cbf.1173; Doussiere J, 1999, BIOCHEMISTRY-US, V38, P16394, DOI 10.1021/bi991502w; Dring MJ, 2006, ADV BOT RES, V43, P175, DOI 10.1016/S0065-2296(05)43004-9; Ellertsdottir E, 1997, MAR ECOL PROG SER, V146, P135, DOI 10.3354/meps146135; Fabbri AA, 2000, J EXP BOT, V51, P1267, DOI 10.1093/jexbot/51.348.1267; Farmer EE, 1998, PLANTA, V206, P167, DOI 10.1007/s004250050388; FARMER EE, 1994, PLANT MOL BIOL, V26, P1423, DOI 10.1007/BF00016483; Ferrante A, 1996, BIOL NEONATE, V69, P368, DOI 10.1159/000244333; Fraser TCM, 2004, PLANT PHYSIOL, V135, P859, DOI 10.1104/pp.104.038984; Fuller MA, 2001, ARCH BIOCHEM BIOPHYS, V388, P146, DOI 10.1006/abbi.2000.2269; Gachon CMM, 2006, EUR J PHYCOL, V41, P395, DOI 10.1080/09670260600960918; Gaquerel E, 2007, BBA-MOL CELL BIOL L, V1771, P565, DOI 10.1016/j.bbalip.2007.02.007; GERWICK WH, 1994, BBA-LIPID LIPID MET, V1211, P243, DOI 10.1016/0005-2760(94)90147-3; GLAZENER JA, 1991, PHYSIOL MOL PLANT P, V39, P123, DOI 10.1016/0885-5765(91)90023-B; Hay ME, 1998, CHEMOECOLOGY, V8, P91, DOI 10.1007/PL00001809; Hay Mark E., 1992, P371; Hay ME, 1996, J EXP MAR BIOL ECOL, V200, P103, DOI 10.1016/S0022-0981(96)02659-7; HAY ME, 1988, ANNU REV ECOL SYST, V19, P111, DOI 10.1146/annurev.es.19.110188.000551; Herve C, 2006, CURR GENET, V49, P190, DOI 10.1007/s00294-005-0044-z; Hofmann M, 1997, PLANT CELL PHYSIOL, V38, P1046, DOI 10.1093/oxfordjournals.pcp.a029270; Howe GA, 2002, CURR OPIN PLANT BIOL, V5, P230, DOI 10.1016/S1369-5266(02)00250-9; Huang ZH, 1997, BIOCHEM J, V325, P553, DOI 10.1042/bj3250553; Jaworek J, 2001, J PHYSIOL PHARMACOL, V52, P107; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; Maier I, 1995, PROGR PHYCOL RES, V11, P51; McHugh DJ., 2003, GUIDE SEAWEED IND; MILLER CC, 1990, J NUTR, V120, P36; Miralto A, 1999, NATURE, V402, P173, DOI 10.1038/46023; Moore BS, 1999, NAT PROD REP, V16, P653, DOI 10.1039/a805873c; Mueller MJ, 1998, CHEM BIOL, V5, pR323, DOI 10.1016/S1074-5521(98)90660-3; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; Mur LAJ, 1997, TRENDS MICROBIOL, V5, P297, DOI 10.1016/S0966-842X(97)01097-4; Orozco-Cardenas M, 1999, P NATL ACAD SCI USA, V96, P6553, DOI 10.1073/pnas.96.11.6553; Orozco-Cardenas ML, 2001, PLANT CELL, V13, P179, DOI 10.1105/tpc.13.1.179; Palmer CJ, 2005, ENVIRON CHEM, V2, P282, DOI 10.1071/EN05078; Pan ZQ, 1998, J BIOL CHEM, V273, P18139, DOI 10.1074/jbc.273.29.18139; Pavia H, 2000, ECOLOGY, V81, P3212; Pompeia C, 2003, BRAZ J MED BIOL RES, V36, P1549, DOI 10.1590/S0100-879X2003001100013; Potin P, 1999, CURR OPIN MICROBIOL, V2, P276, DOI 10.1016/S1369-5274(99)80048-4; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Reymond P, 1998, CURR OPIN PLANT BIOL, V1, P404, DOI 10.1016/S1369-5266(98)80264-1; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Ross C, 2006, CHEM BIOL, V13, P353, DOI 10.1016/j.chembiol.2006.01.009; Ross C, 2005, J PHYCOL, V41, P531, DOI 10.1111/j.1529-8817.2005.04072.x; RUBINEK T, 1993, BIOCHIM BIOPHYS ACTA, V1176, P51, DOI 10.1016/0167-4889(93)90176-P; Sawabe T, 2000, INT J SYST EVOL MICR, V50, P265, DOI 10.1099/00207713-50-1-265; Sawabe T, 1998, INT J SYST BACTERIOL, V48, P769, DOI 10.1099/00207713-48-3-769; Schnitzler I, 2001, OECOLOGIA, V126, P515, DOI 10.1007/s004420000546; Schnitzler I, 1998, J CHEM ECOL, V24, P1715, DOI 10.1023/A:1020876830580; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Shiose A, 2000, J BIOL CHEM, V275, P13793, DOI 10.1074/jbc.275.18.13793; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Steinberg Peter D., 1992, P51; STRATMANN K, 1992, ANGEW CHEM, V104, P1261; Thoma I, 2003, PLANT J, V34, P363, DOI 10.1046/j.1365-313X.2003.01730.x; Toth GB, 2000, P NATL ACAD SCI USA, V97, P14418, DOI 10.1073/pnas.250226997; Weinberger F, 1999, J PHYCOL, V35, P747, DOI 10.1046/j.1529-8817.1999.3540747.x; Weinberger F, 2000, J APPL PHYCOL, V12, P139, DOI 10.1023/A:1008119125911; Weinberger F, 2000, J PHYCOL, V36, P1079, DOI 10.1046/j.1529-8817.2000.00003.x; Wojtaszek P, 1997, BIOCHEM J, V322, P681, DOI 10.1042/bj3220681; Wong RKM, 2003, CIRCULATION, V108, P1858, DOI 10.1161/01.CIR.0000089372.64585.3B; Yaeno T, 2004, PLANT J, V40, P931, DOI 10.1111/j.1365-313X.2004.02260.x; Yoshioka H, 2001, MOL PLANT MICROBE IN, V14, P725, DOI 10.1094/MPMI.2001.14.6.725	84	73	78	1	26	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0032-0781	1471-9053		PLANT CELL PHYSIOL	Plant Cell Physiol.	APR	2009	50	4					789	800		10.1093/pcp/pcp023			12	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	434LT	WOS:000265276900010	19213737	Other Gold			2021-04-07	
J	Schroeder, DC; Park, Y; Yoon, HM; Lee, YS; Kang, W; Meints, RH; Ivey, RG; Choi, TJ				Schroeder, Declan C.; Park, Yunjung; Yoon, Hong-Mook; Lee, Yong Seok; Kang, Won; Meints, Russel H.; Ivey, Richard G.; Choi, Tae-Jin			Genomic analysis of the smallest giant virus - Feldmannia sp virus 158	VIROLOGY			English	Article						Algae; Feldmannia; FsV; Phaeoviruses; Phycodnaviridae; NCLDV	BROWN ALGAL VIRUS; ECTOCARPUS; HOST; INFECTION; PROTEIN; GENE; IDENTIFICATION; PHAEOPHYCEAE; INTEGRATION; CONTAINS	Genomic analysis of Feldmannia sp. virus 158, the second phaeovirus to be sequenced in its entirety, provides further evidence that large double-stranded DNA viruses share similar evolutionary pressures as cellular organisms. Reductive evolution is clearly evident within the phaeoviruses which occurred via several routes: the loss of genes from an ancestral virus core genome most likely through genetic drift; and as a result of relatively large recombination events that caused wholesale loss of genes. The entire genome is 154,641 by in length and has 150 predicted coding sequences of which 87% have amino acid sequence similarities to other algal virus coding sequences within the family Phycodnaviridae. Significant similarities were found, for thirty eight coding sequences (25%), to genes in gene databanks that are known to be involved in processes that include DNA replication, DNA methylation, signal transduction, viral integration and transposition, and protein-protein interactions. Unsurprisingly, the greatest similarity was observed between the two known viruses that infect Feldmannia, indicating the taxonomic linkage of these two viruses with their hosts. Moreover, comparative analysis of phycodnaviral genomic sequences revealed the smallest set of core genes (10 out of a possible 31) required to make a functional nucleocytoplasmic large dsDNA virus. (c) 2008 Elsevier Inc. All rights reserved.	[Park, Yunjung; Yoon, Hong-Mook; Choi, Tae-Jin] Pukyong Natl Univ, Dept Microbiol, Pusan 608737, South Korea; [Schroeder, Declan C.] Marine Biol Assoc UK, Plymouth PL1 2PB, Devon, England; [Lee, Yong Seok; Kang, Won] Inje Univ, Coll Med & Frontier Inje Res Sci & Technol, PICR, Dept Parasitol & Malariol, Pusan 614735, South Korea; [Meints, Russel H.; Ivey, Richard G.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA	Choi, TJ (corresponding author), Pukyong Natl Univ, Dept Microbiol, 599-1 Daeyeon,3 Dong, Pusan 608737, South Korea.	choitj@pknu.ac.kr	Schroeder, Declan C./O-9131-2019	Schroeder, Declan C./0000-0001-5991-2838; Lee, Yongseok/0000-0002-8687-589X	Ministry of Science and Technology of Korean governmentMinistry of Science and Technology (MOST) Korea [PF0330601-00]; NERCUK Research & Innovation (UKRI)NERC Natural Environment Research Council [R8-H12-52]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [mba010002] Funding Source: researchfish	This research was supported by a grant (PF0330601-00) from the Plant Diversity Research Center of 21st Century Frontier Research Program funded by the Ministry of Science and Technology of Korean government. DCS is an MBA Research Fellow funded by grant in aid from NERC and through the NERC core strategic research programme Oceans2025 (R8-H12-52).	Aihara H, 2007, MOL CELL, V27, P901, DOI 10.1016/j.molcel.2007.07.026; Allen MJ, 2006, MOL BIOL EVOL, V23, P86, DOI 10.1093/molbev/msj010; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; Besemer J, 2005, NUCLEIC ACIDS RES, V33, pW451, DOI 10.1093/nar/gki487; Bocs S, 2003, NUCLEIC ACIDS RES, V31, P3723, DOI 10.1093/nar/gkg590; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Claverie JM, 2006, VIRUS RES, V117, P133, DOI 10.1016/j.virusres.2006.01.008; Del Campo E, 1997, PHYCOLOGIA, V36, P186, DOI 10.2216/i0031-8884-36-3-186.1; Delaroque N, 2003, J MOL EVOL, V57, P613, DOI 10.1007/s00239-003-2501-y; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Delaroque N, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-110; Delcher AL, 1999, NUCLEIC ACIDS RES, V27, P4636, DOI 10.1093/nar/27.23.4636; Dunigan DD, 2006, VIRUS RES, V117, P119, DOI 10.1016/j.virusres.2006.01.024; Felsenstein J., 1995, PHYLIP PHYLOGENY INF; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Iyer LM, 2001, J VIROL, V75, P11720, DOI 10.1128/JVI.75.23.11720-11734.2001; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; KUHLENKAMP R, 1994, BOT MAR, V37, P525, DOI 10.1515/botm.1994.37.6.525; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LEE AM, 1995, VIROLOGY, V212, P474, DOI 10.1006/viro.1995.1505; Lee AM, 1998, J PHYCOL, V34, P608, DOI 10.1046/j.1529-8817.1998.340608.x; Lee AM, 1998, VIROLOGY, V248, P35, DOI 10.1006/viro.1998.9245; Maier I, 1998, EUR J PHYCOL, V33, P213, DOI 10.1017/S0967026298001747; Meints RH, 2008, J VIROL, V82, P1407, DOI 10.1128/JVI.01983-07; Muller DG, 1996, HYDROBIOLOGIA, V327, P21; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, PROTOPLASMA, V193, P58, DOI 10.1007/BF01276634; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; Page RDM, 1996, COMPUT APPL BIOSCI, V12, P357; Park Y, 2007, VIRUS GENES, V34, P177, DOI 10.1007/s11262-006-0059-7; Pickart CM, 2001, ANNU REV BIOCHEM, V70, P503, DOI 10.1146/annurev.biochem.70.1.503; Raoult D, 2004, SCIENCE, V306, P1344, DOI 10.1126/science.1101485; Ravin NV, 2003, NUCLEIC ACIDS RES, V31, P6552, DOI 10.1093/nar/gkg856; Rutherford K, 2000, BIOINFORMATICS, V16, P944, DOI 10.1093/bioinformatics/16.10.944; Sambrook J., 1989, MOL CLONING LAB MANU; Stajich JE, 2002, GENOME RES, V12, P1611, DOI 10.1101/gr.361602; Tatusov RL, 2000, NUCLEIC ACIDS RES, V28, P33, DOI 10.1093/nar/28.1.33; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Voulvoulis N, 1999, APPL ORGANOMET CHEM, V13, P135, DOI 10.1002/(SICI)1099-0739(199903)13:3<135::AID-AOC831>3.3.CO;2-7; WILSON W, 2005, VIRUS TAXONOMY CLASS; Yoon HS, 2004, MOL BIOL EVOL, V21, P809, DOI 10.1093/molbev/msh075	49	23	24	0	10	ACADEMIC PRESS INC ELSEVIER SCIENCE	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA	0042-6822			VIROLOGY	Virology	FEB 5	2009	384	1					223	232		10.1016/j.virol.2008.10.040			10	Virology	Virology	402US	WOS:000263039200028	19054537				2021-04-07	
J	Gachon, CMM; Strittmatter, M; Muller, DG; Kleinteich, J; Kupper, FC				Gachon, Claire M. M.; Strittmatter, Martina; Mueller, Dieter G.; Kleinteich, Julia; Kuepper, Frithjof C.			Detection of Differential Host Susceptibility to the Marine Oomycete Pathogen Eurychasma dicksonii by Real-Time PCR: Not All Algae Are Equal	APPLIED AND ENVIRONMENTAL MICROBIOLOGY			English	Article							ECTOCARPUS-SILICULOSUS; MOLECULAR PHYLOGENY; ARABIDOPSIS; VIRUSES; CHYTRIDIUM; MORPHOLOGY; DIVERSITY	In the marine environment, a growing body of evidence points to parasites as key players in the control of population dynamics and overall ecosystem structure. However, their prevalence and impact on marine macroalgal communities remain virtually unknown. Indeed, infectious diseases of seaweeds are largely underdocumented, partly because of the expertise required to diagnose them with a microscope. Over the last few years, however, real-time quantitative PCR (qPCR) has emerged as a rapid and reliable alternative to visual symptom scoring for monitoring pathogens. Thus, we present here a qPCR assay suitable for the detection and quantification of the intracellular oomycete pathogen Eurychasma dicksonii in its ectocarpalean and laminarialean brown algal hosts. qPCR and microscopic observations made of laboratory-controlled cultures revealed that clonal brown algal strains exhibit different levels of resistance against Eurychasma, ranging from high susceptibility to complete absence of symptoms. This observation strongly argues for the existence of a genetic determinism for disease resistance in brown algae, which would have broad implications for the dynamics and genetic structure of natural populations. We also used qPCR for the rapid detection of Eurychasma in filamentous brown algae collected in Northern Europe and South America and found that the assay is specific, robust, and widely applicable to field samples. Hence, this study opens the perspective of combining large-scale disease monitoring in the field with laboratory-controlled experiments on the genome model seaweed Ectocarpus siliculosus to improve our understanding of brown algal diseases.	[Gachon, Claire M. M.; Strittmatter, Martina; Kuepper, Frithjof C.] Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Culture Collect Algae & Protozoa, Oban PA37 1QA, Argyll, Scotland; [Mueller, Dieter G.; Kleinteich, Julia] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany	Gachon, CMM (corresponding author), Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Culture Collect Algae & Protozoa, Oban PA37 1QA, Argyll, Scotland.	cmmg@sams.ac.uk	Gachon, Claire/C-2787-2009	Kuepper, Frithjof/0000-0003-1273-7109; Strittmatter, Martina/0000-0002-1258-9751; Gachon, Claire/0000-0002-3702-7472	European CommissionEuropean CommissionEuropean Commission Joint Research Centre [MIEF-CT-2006-022837, MEST-CT-2005-20501]; UK Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/D521522/1, MGF 154]; Marine and Freshwater Microbial Biodiversity Programme and Oceans [2025/WP4.5]; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NBAF010003, NE/D521522/1] Funding Source: researchfish	C. M. M. G. expresses her gratitude to the European Commission for a Marie Curie Intra-European Fellowship (MIEF-CT-2006-022837). M. S. is the recipient of an EU ECOSUMMER Ph.D. fellowship (MEST-CT-2005-20501). F. C. K. gratefully acknowledges funding from the UK Natural Environment Research Council (NERC; grant NE/D521522/1, International Opportunities Fund of the Marine and Freshwater Microbial Biodiversity Programme, and Oceans 2025/WP4.5) and a sequencing allocation from the NERC National Sequencing Facility (MGF 154). Debra Brennan is acknowledged for her excellent technical assistance.	ALEEM A, 1955, SWEDEN ARK BOT, V3, P1; Allen RL, 2004, SCIENCE, V306, P1957, DOI 10.1126/science.1104022; Bouarab K, 2001, CAH BIOL MAR, V42, P91; CANTER HM, 1979, NEW PHYTOL, V82, P187, DOI 10.1111/j.1469-8137.1979.tb07574.x; Carey C, 2000, ENVIRON HEALTH PERSP, V108, P143, DOI 10.2307/3454639; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; De Bruin A, 2004, J PHYCOL, V40, P823, DOI 10.1111/j.1529-8817.2004.04006.x; Duffy MA, 2007, ECOL LETT, V10, P44, DOI 10.1111/j.1461-0248.2006.00995.x; Fuhrman JA, 1999, NATURE, V399, P541, DOI 10.1038/21119; Gachon C, 2004, J EXP BOT, V55, P1445, DOI 10.1093/jxb/erh181; Gachon C, 2004, PLANT PHYSIOL BIOCH, V42, P367, DOI 10.1016/j.plaphy.2004.04.001; Gachon CMM, 2007, GENE, V406, P51, DOI 10.1016/j.gene.2007.05.018; GRAHAME E, 1976, JAMAICA BR PHYCOL J, V11, P57; Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.5598/IMAFUNGUS.2011.02.02.06; Harvell CD, 1999, SCIENCE, V285, P1505, DOI 10.1126/science.285.5433.1505; Hoarau G, 2007, MOL ECOL NOTES, V7, P191, DOI 10.1111/j.1471-8286.2006.01587.x; Holfeld H, 1998, NEW PHYTOL, V138, P507, DOI 10.1046/j.1469-8137.1998.00126.x; Holub EB, 2007, CURR OPIN PLANT BIOL, V10, P415, DOI 10.1016/j.pbi.2007.05.003; HUGHES TP, 1994, SCIENCE, V265, P1547, DOI 10.1126/science.265.5178.1547; Jones KE, 2008, NATURE, V451, P990, DOI 10.1038/nature06536; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Marin B, 2003, PROTIST, V154, P99, DOI 10.1078/143446103764928521; Martinez JM, 2007, APPL ENVIRON MICROB, V73, P554, DOI 10.1128/AEM.00864-06; Muhling M, 2005, ENVIRON MICROBIOL, V7, P499, DOI 10.1111/j.1462-2920.2005.00713.x; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Rose LE, 2004, GENETICS, V166, P1517, DOI 10.1534/genetics.166.3.1517; Sekimoto S, 2008, PROTIST, V159, P299, DOI 10.1016/j.protis.2007.11.004; Sekimoto S, 2008, MYCOL RES, V112, P361, DOI 10.1016/j.mycres.2007.11.002; Sparrow FK, 1960, AQUATIC PHYCOMYCETES, V15; STARR R, 1993, J PHYCOL, V29, pS1; STRITTMATTER M, OOMYCETE GE IN PRESS; Suttle CA, 2005, NATURE, V437, P356, DOI 10.1038/nature04160; Tillmann U, 1999, J SEA RES, V42, P255, DOI 10.1016/S1385-1101(99)00029-5; West JA, 2006, PHYCOL RES, V54, P72, DOI 10.1111/j.1440-1835.2006.00410.x; WILCE RT, 1982, PHYCOLOGIA, V21, P336, DOI 10.2216/i0031-8884-21-3-336.1	38	58	61	0	16	AMER SOC MICROBIOLOGY	WASHINGTON	1752 N ST NW, WASHINGTON, DC 20036-2904 USA	0099-2240	1098-5336		APPL ENVIRON MICROB	Appl. Environ. Microbiol.	JAN 15	2009	75	2					322	328		10.1128/AEM.01885-08			7	Biotechnology & Applied Microbiology; Microbiology	Biotechnology & Applied Microbiology; Microbiology	390XT	WOS:000262197700004	19011072	Bronze, Green Published			2021-04-07	
J	Dittami, SM; Scornet, D; Petit, JL; Segurens, B; Da Silva, C; Corre, E; Dondrup, M; Glatting, KH; Konig, R; Sterck, L; Rouze, P; Van de Peer, Y; Cock, JM; Boyen, C; Tonon, T				Dittami, Simon M.; Scornet, Delphine; Petit, Jean-Louis; Segurens, Beatrice; Da Silva, Corinne; Corre, Erwan; Dondrup, Michael; Glatting, Karl-Heinz; Koenig, Rainer; Sterck, Lieven; Rouze, Pierre; Van de Peer, Yves; Cock, J. Mark; Boyen, Catherine; Tonon, Thierry			Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress	GENOME BIOLOGY			English	Article							LAMINARIA-DIGITATA PHAEOPHYCEAE; FALSE DISCOVERY RATE; PROBE LEVEL DATA; GENE-EXPRESSION; MICROARRAY DATA; RNA AMPLIFICATION; ARABIDOPSIS GENES; CHONDRUS-CRISPUS; MODEL ORGANISMS; CDNA MICROARRAY	Background: Brown algae (Phaeophyceae) are phylogenetically distant from red and green algae and an important component of the coastal ecosystem. They have developed unique mechanisms that allow them to inhabit the intertidal zone, an environment with high levels of abiotic stress. Ectocarpus siliculosus is being established as a genetic and genomic model for the brown algal lineage, but little is known about its response to abiotic stress. Results: Here we examine the transcriptomic changes that occur during the short term acclimation of E. siliculosus to three different abiotic stress conditions (hyposaline, hypersaline and oxidative stress). Our results show that almost 70% of the expressed genes are regulated in response to at least one of these stressors. Although there are several common elements with terrestrial plants, such as repression of growth-related genes, switching from primary production to protein and nutrient recycling processes, and induction of genes involved in vesicular trafficking, many of the stress-regulated genes are either not known to respond to stress in other organisms or are have been found exclusively in E. siliculosus. Conclusions: This first large-scale transcriptomic study of a brown alga demonstrates that, unlike terrestrial plants, E. siliculosus undergoes extensive reprogramming of its transcriptome during the acclimation to mild abiotic stress. We identify several new genes and pathways with a putative function in the stress response and thus pave the way for more detailed investigations of the mechanisms underlying the stress tolerance of brown algae.	[Dittami, Simon M.; Scornet, Delphine; Cock, J. Mark; Boyen, Catherine; Tonon, Thierry] Univ Paris 06, UPMC, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29680 Roscoff, France; [Dittami, Simon M.; Scornet, Delphine; Cock, J. Mark; Boyen, Catherine; Tonon, Thierry] CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29680 Roscoff, France; [Petit, Jean-Louis; Segurens, Beatrice; Da Silva, Corinne] Genoscope, Inst Genom, DSV, CEA, F-91057 Evry, France; [Petit, Jean-Louis; Segurens, Beatrice; Da Silva, Corinne] CNRS, UMR 8030, F-91057 Evry, France; [Petit, Jean-Louis; Segurens, Beatrice; Da Silva, Corinne] Univ Evry, F-91057 Evry, France; [Corre, Erwan] UPMC, CNRS, SIG FR 2424, Biol Stn, F-29680 Roscoff, France; [Dondrup, Michael] Univ Bielefeld, Ctr Biotechnol CeBiTec, D-33594 Bielefeld, Germany; [Glatting, Karl-Heinz; Koenig, Rainer] German Canc Res Ctr, D-69120 Heidelberg, Germany; [Sterck, Lieven; Rouze, Pierre; Van de Peer, Yves] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium	Dittami, SM (corresponding author), Univ Paris 06, UPMC, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29680 Roscoff, France.	dittami@sb-roscoff.fr; scornet@sb-roscoff.fr; petit@genoscope.cns.fr; segurens@genoscope.cns.fr; dasilva@genoscope.cns.fr; corre@sb-roscoff.fr; mdondrup@cebitec.uni-bielefeld.de; glatting@dkfz-heidelberg.de; r.koenig@dkfz-heidelberg.de; lieven.sterck@psb.vib-ugent.be; pierre.rouze@psb.vib-ugent.be; yves.vandepeer@psb.vib-ugent.be; cock@sb-roscoff.fr; boyen@sb-roscoff.fr; tonon@sb-roscoff.fr	Van de Peer, Yves/AAE-7666-2019; Tonon, Thierry/A-3214-2009; corre, erwan/O-4669-2019; Sterck, Lieven/A-9439-2016; Dittami, Simon/E-8354-2011; Van de Peer, Yves/D-4388-2009	Van de Peer, Yves/0000-0003-4327-3730; Tonon, Thierry/0000-0002-1454-6018; corre, erwan/0000-0001-6354-2278; Sterck, Lieven/0000-0001-7116-4000; Dittami, Simon/0000-0001-7987-7523; Van de Peer, Yves/0000-0003-4327-3730; DA SILVA, Corinne/0000-0002-7618-7831; Cock, J. Mark/0000-0002-2650-0383; Dondrup, Michael/0000-0002-2371-5928; Petit, Jean-Louis/0000-0002-8566-0571			Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Ashburner M, 2000, NAT GENET, V25, P25, DOI 10.1038/75556; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Bassham DC, 2007, CURR OPIN PLANT BIOL, V10, P587, DOI 10.1016/j.pbi.2007.06.006; Belknap WR, 1996, TRENDS PLANT SCI, V1, P331; Benjamini Y, 2001, ANN STAT, V29, P1165; BENJAMINI Y, 1995, J R STAT SOC B, V57, P289, DOI 10.1111/j.2517-6161.1995.tb02031.x; Blumwald E, 2000, CURR OPIN CELL BIOL, V12, P431, DOI 10.1016/S0955-0674(00)00112-5; Bolstad BM, 2003, BIOINFORMATICS, V19, P185, DOI 10.1093/bioinformatics/19.2.185; Bouchereau A, 1999, PLANT SCI, V140, P103, DOI 10.1016/S0168-9452(98)00218-0; Boyen C, 2001, CAH BIOL MAR, V42, P11; BUTLER DM, 1989, J EXP BOT, V40, P1237, DOI 10.1093/jxb/40.11.1237; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Colin C, 2003, J BIOL CHEM, V278, P23545, DOI 10.1074/jbc.M300247200; Collen J, 1999, J PHYCOL, V35, P62, DOI 10.1046/j.1529-8817.1999.3510062.x; Collen J, 2007, NEW PHYTOL, V176, P45, DOI 10.1111/j.1469-8137.2007.02152.x; Collen J, 2006, J EXP BOT, V57, P3869, DOI 10.1093/jxb/erl171; Cosse A., 2007, ADV BOT RES, V46, P221, DOI DOI 10.1016/S0065-2296(07)46006-2; DAVISON IR, 1985, PHYCOLOGIA, V24, P449, DOI 10.2216/i0031-8884-24-4-449.1; Davison IR, 1996, J PHYCOL, V32, P197, DOI 10.1111/j.0022-3646.1996.00197.x; Eastmond PJ, 2001, TRENDS PLANT SCI, V6, P72, DOI 10.1016/S1360-1385(00)01835-5; Ewing B, 1998, GENOME RES, V8, P175, DOI 10.1101/gr.8.3.175; FOYER CH, 1994, PHYSIOL PLANTARUM, V92, P696, DOI 10.1111/j.1399-3054.1994.tb03042.x; Fujita M, 2006, CURR OPIN PLANT BIOL, V9, P436, DOI 10.1016/j.pbi.2006.05.014; GEISSLER U, 1983, NOVA HEDWIGIA, V37, P193; Gerwick WH, 1999, ADV EXP MED BIOL, V447, P211; Graham MH, 2007, P NATL ACAD SCI USA, V104, P16576, DOI 10.1073/pnas.0704778104; Gschloessl B, 2008, BMC BIOINFORMATICS, V9, DOI 10.1186/1471-2105-9-393; Hwang YS, 2008, BIOCHEM BIOPH RES CO, V367, P635, DOI 10.1016/j.bbrc.2007.12.176; Irizarry RA, 2003, BIOSTATISTICS, V4, P249, DOI 10.1093/biostatistics/4.2.249; Irizarry RA, 2003, NUCLEIC ACIDS RES, V31, DOI 10.1093/nar/gng015; Katsuta Hitoshi, 2005, Medical Molecular Morphology, V38, P30, DOI 10.1007/s00795-004-0270-3; Kimura M, 2003, PHOTOCHEM PHOTOBIOL, V77, P226, DOI 10.1562/0031-8655(2003)077&lt;0226:IOAGRB&gt;2.0.CO;2; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; Kourtis N, 2009, CELL DEATH DIFFER, V16, P21, DOI 10.1038/cdd.2008.120; Kovtun Y, 2000, P NATL ACAD SCI USA, V97, P2940, DOI 10.1073/pnas.97.6.2940; Krell A, 2007, J PHYCOL, V43, P753, DOI 10.1111/j.1529-8817.2007.00366.x; Kreps JA, 2002, PLANT PHYSIOL, V130, P2129, DOI 10.1104/pp.008532; Kupper FC, 2008, P NATL ACAD SCI USA, V105, P6954, DOI 10.1073/pnas.0709959105; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Lipka V, 2007, ANNU REV CELL DEV BI, V23, P147, DOI 10.1146/annurev.cellbio.23.090506.123529; Livesey FJ, 2000, CURR BIOL, V10, P301, DOI 10.1016/S0960-9822(00)00379-1; MILPETZ F, 1995, TRENDS BIOCHEM SCI, V20, P204, DOI 10.1016/S0968-0004(00)89009-X; Min XJ, 2005, NUCLEIC ACIDS RES, V33, pW677, DOI 10.1093/nar/gki394; Mittler R, 2004, TRENDS PLANT SCI, V9, P490, DOI 10.1016/j.tplants.2004.08.009; Miyama M, 2008, PLANT MOL BIOL, V68, P119, DOI 10.1007/s11103-008-9356-y; Morris SM, 2004, CURR OPIN CLIN NUTR, V7, P45, DOI 10.1097/00075197-200401000-00009; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Pertea G, 2003, BIOINFORMATICS, V19, P651, DOI 10.1093/bioinformatics/btg034; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Puskas LG, 2002, BIOTECHNIQUES, V32, P1330, DOI 10.2144/02326mt04; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; RITTER A, 2008, NEW PHYTOL; Rodriguez-Manzaneque MT, 1999, MOL CELL BIOL, V19, P8180; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Saeed AI, 2003, BIOTECHNIQUES, V34, P374, DOI 10.2144/03342mt01; Santelices B, 2007, P NATL ACAD SCI USA, V104, P19163, DOI 10.1073/pnas.0708963104; Sealfon RSG, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-443; Seki M, 2002, PLANT J, V31, P279, DOI 10.1046/j.1365-313X.2002.01359.x; Shannon P, 2003, GENOME RES, V13, P2498, DOI 10.1101/gr.1239303; SOEDER C, 1974, BOT MONOGRAPHS; Soukas A, 2000, GENE DEV, V14, P963; Spirin KS, 1999, INVEST OPHTH VIS SCI, V40, P3108; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stirewalt DL, 2004, GENOMICS, V83, P321, DOI 10.1016/j.ygeno.2003.08.008; Tonon T, 2008, J PHYCOL, V44, P1250, DOI 10.1111/j.1529-8817.2008.00580.x; Vinayagam A, 2006, BMC BIOINFORMATICS, V7, DOI 10.1186/1471-2105-7-161; vonKampen J, 1996, PHYSIOL PLANTARUM, V97, P618, DOI 10.1111/j.1399-3054.1996.tb00523.x; Wang E, 2000, NAT BIOTECHNOL, V18, P457; Wang WX, 2004, TRENDS PLANT SCI, V9, P244, DOI 10.1016/j.tplants.2004.03.006; West John A., 1996, Muelleria, V9, P29; Wu JM, 2006, NUCLEIC ACIDS RES, V34, pW720, DOI 10.1093/nar/gkl167; YAMAGUCH.T, 1969, PLANT CELL PHYSIOL, V10, P425; Yeung KY, 2001, BIOINFORMATICS, V17, P309, DOI 10.1093/bioinformatics/17.4.309; Zhumabayeva B, 2001, BIOTECHNIQUES, V30, P158, DOI 10.2144/01301pf01	80	99	104	0	33	BIOMED CENTRAL LTD	LONDON	236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND	1474-760X			GENOME BIOL	Genome Biol.		2009	10	6							R66	10.1186/gb-2009-10-6-r66			51	Biotechnology & Applied Microbiology; Genetics & Heredity	Biotechnology & Applied Microbiology; Genetics & Heredity	465RK	WOS:000267604300016	19531237	DOAJ Gold, Green Published			2021-04-07	
J	Wiesemeier, T; Jahn, K; Pohnert, G				Wiesemeier, Theresa; Jahn, Karsten; Pohnert, Georg			No Evidence for the Induction of Brown Algal Chemical Defense by the Phytohormones Jasmonic Acid and Methyl Jasmonate	JOURNAL OF CHEMICAL ECOLOGY			English	Article						Dictyota dichotoma; Dictyotales; Induced defense; Metabolic profiling; Metabolomics; Terpenes; Jasmonic acid; Methyl jasmonate; Brown algae; Phytohormones	PLANT STRESS-RESPONSE; DICTYOTA-DICHOTOMA; FUCUS-VESICULOSUS; PHASEOLUS-LUNATUS; RED ALGA; RECOGNITION; HERBIVORES; RESISTANCE; DITERPENE; QUANTIFICATION	Induced chemical defense reactions are widespread in marine brown algae. Despite the evidence that the biosynthesis of defense metabolites can be up-regulated upon herbivory, we do not know how this regulation of biosynthetic pathways to secondary metabolites is achieved in brown algae. In higher plants, the phytohormone jasmonic acid (JA) is crucial for the mediation of induced chemical defenses, and several findings of this metabolite from marine sources have been reported. We tested the hypothesis that JA or related metabolites play a role in induced brown algal defense. Quantification of oxylipins with a detection limit around 20 ng g-1 algal tissue did not reveal the presence of JA in the seven examined brown algal species Dictyota dichotoma, Colpomenia peregrina, Ectocarpus fasciculatus, Fucus vesiculosus, Himanthalia elongata, Saccharina latissima (formerly Laminaria saccharina), and Sargassum muticum. Moreover, treatment with ecologically relevant concentrations of JA and methyl jasmonate did not lead to a significant change in the profile of medium- and non-polar metabolites of the tested algae. Only when high concentrations of >= 500 mu g ml-1 medium of the phytohormones were applied that a metabolic response which could be attributed to unspecific stress was observed. Bioassays with D. dichotoma that focused on medium- and non-polar compounds confirmed the lack of a biological role of JA and methyl jasmonate in the induction of algal induced chemical defenses. The phytohormone-treated samples did not exhibit any increased defense potential towards the amphipod Ampithoe longimana and the isopod Paracerceis caudata. JA and related phytohormones, known to be active in higher plants, thus appear to play no role in brown algae for induction of the defense chemicals studied here.	[Wiesemeier, Theresa; Jahn, Karsten; Pohnert, Georg] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, Lab Chem Ecol, CH-1015 Lausanne, Switzerland	Pohnert, G (corresponding author), Univ Jena, Inst Anorgan & Analyt Chem, Lessingstr 8, D-07743 Jena, Germany.	georg.pohnert@uni-jena.de	Pohnert, Georg/D-3721-2013; Lenka, Sangram K./A-5830-2009	Pohnert, Georg/0000-0003-2351-6336; Lenka, Sangram K./0000-0002-0121-2430	VolkswagenstiftungVolkswagen	We thank Akira Peters and Florian Weinberger for their help with algal collection and identification. We are grateful to Marc Hay, who provided us with test animals and introduced us to the techniques for bioassays. Paulina Dabrowska and Birgit Schulze are acknowledged for help with analysis of phytohormones. We acknowledge the helpful support from Wilhelm Boland and funding from the Volkswagenstiftung.	ADRA C, 2006, J AGR FOOD CHEM, V54, P9317; Arnold TM, 2001, J PHYCOL, V37, P1026, DOI 10.1046/j.1529-8817.2001.01130.x; Baldwin IT, 2002, CURR OPIN PLANT BIOL, V5, P351, DOI 10.1016/S1369-5266(02)00263-7; Barbosa JP, 2004, BOT MAR, V47, P147, DOI 10.1515/BOT.2004.015; BOLAND W, 1995, ANGEW CHEM INT EDIT, V34, P1600, DOI 10.1002/anie.199516001; Bouarab K, 2004, PLANT PHYSIOL, V135, P1838, DOI 10.1104/pp.103.037622; Christov C, 2001, BIOL PLANTARUM, V44, P367, DOI 10.1023/A:1012490610127; Cronin G, 1996, ECOLOGY, V77, P2287, DOI 10.2307/2265731; Cruz-Rivera E, 2003, ECOL MONOGR, V73, P483, DOI 10.1890/0012-9615(2003)073[0483:PNQIWC]2.0.CO;2; Czerpak R, 2006, ACTA PHYSIOL PLANT, V28, P195, DOI 10.1007/BF02706531; FATTORUSSO E, 1976, J CHEM SOC CHEM COMM, P575, DOI 10.1039/c39760000575; Feussner I, 2002, ANNU REV PLANT BIOL, V53, P275, DOI 10.1146/annurev.arplant.53.100301.135248; Fu M, 2004, TOXICON, V43, P355, DOI 10.1016/j.toxicon.2003.09.012; Garcia-Brugger A, 2006, MOL PLANT MICROBE IN, V19, P711, DOI 10.1094/MPMI-19-0711; Heil M, 2004, J ECOL, V92, P527, DOI 10.1111/j.0022-0477.2004.00890.x; Hemmi A, 2004, MAR ECOL PROG SER, V273, P109, DOI 10.3354/meps273109; HOPKE J, 1994, FEBS LETT, V352, P146, DOI 10.1016/0014-5793(94)00948-1; Juttner F, 2001, J PHYCOL, V37, P744, DOI 10.1046/j.1529-8817.2001.00130.x; Karban R., 1997, INDUCED RESPONSES HE; Keinanen M, 2001, J AGR FOOD CHEM, V49, P3553, DOI 10.1021/jf010200+; Kim JY, 2006, BIOSCI BIOTECH BIOCH, V70, P2571, DOI 10.1271/bbb.60281; Koivikko R, 2008, J CHEM ECOL, V34, P57, DOI 10.1007/s10886-007-9410-2; KRUPINA MV, 1991, Z NATURFORSCH C, V46, P1127; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Macaya EC, 2005, J EXP MAR BIOL ECOL, V325, P214, DOI 10.1016/j.jembe.2005.05.004; Mithofer A, 2005, PLANT PHYSIOL, V137, P1160, DOI 10.1104/pp.104.054460; Molis M, 2006, J ECOL, V94, P243, DOI 10.1111/j.1365-2745.2005.01058.x; Pavia H, 2000, ECOLOGY, V81, P3212; Pavia H, 2002, ECOLOGY, V83, P891; Pohnert G, 2004, TOP CURR CHEM, V239, P179, DOI 10.1007/b95453; Schuler G, 2004, FEBS LETT, V563, P17, DOI 10.1016/S0014-5793(04)00239-X; Schulze B, 2006, ANAL BIOCHEM, V348, P269, DOI 10.1016/j.ab.2005.10.021; Shulaev V, 2008, PHYSIOL PLANTARUM, V132, P199, DOI 10.1111/j.1399-3054.2007.01025.x; Tarakhovskaya ER, 2007, RUSS J PLANT PHYSL+, V54, P163, DOI 10.1134/S1021443707020021; Teixeira VL, 2001, BIOCHEM SYST ECOL, V29, P313, DOI 10.1016/S0305-1978(00)00055-7; Wasternack C, 2007, ANN BOT-LONDON, V100, P681, DOI 10.1093/aob/mcm079; Weckwerth W, 2005, DRUG DISCOV TODAY, V10, P1551, DOI 10.1016/S1359-6446(05)03609-3; Wichard T, 2005, J CHROMATOGR B, V814, P155, DOI 10.1016/j.jchromb.2004.10.021; Wiesemeier T, 2007, AQUAT SCI, V69, P403, DOI 10.1007/s00027-007-0889-y; Wittstock U, 2002, CURR OPIN PLANT BIOL, V5, P300, DOI 10.1016/S1369-5266(02)00264-9	40	16	17	2	39	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0098-0331			J CHEM ECOL	J. Chem. Ecol.	DEC	2008	34	12					1523	1531		10.1007/s10886-008-9568-2			9	Biochemistry & Molecular Biology; Ecology	Biochemistry & Molecular Biology; Environmental Sciences & Ecology	379QL	WOS:000261411200003	19020937				2021-04-07	
J	Le Bail, A; Billoud, B; Maisonneuve, C; Peters, AF; Cock, JM; Charrier, B				Le Bail, Aude; Billoud, Bernard; Maisonneuve, Carole; Peters, Akira F.; Cock, J. Mark; Charrier, Benedicte			EARLY DEVELOPMENT PATTERN OF THE BROWN ALGA ECTOCARPUS SILICULOSUS (ECTOCARPALES, PHAEOPHYCEAE) SPOROPHYTE	JOURNAL OF PHYCOLOGY			English	Article						branching; development; differentiation; filament; macroalga; morphogenesis; morphometry; radial; statistical analyses; symmetry	FUCUS-DISTICHUS; GROWTH; MORPHOGENESIS; CERAMIACEAE; ELONGATION; SIMULATION; CYTOKININS; POLARITY	The distant phylogenetic position of brown macroalgae from the other multicellular phyla offers the opportunity to study novel and alternative developmental processes involved in the establishment of multicellularity. At present, however, very little information is available about developmental patterning in this group. Ectocarpus siliculosus (Dillwyn) Lyngb. has uniseriate filaments and displays one of the simplest architectures in the Phaeophyceae. The aim of this study was to decipher the morphogenetic steps that lead to the development of the Ectocarpus sporophyte. We carried out a detailed morphometric study of the events that occurred between gamete germination and the 100-cell stage. This analysis was performed on two ecologically distant isolates to assess plasticity in developmental patterning within this species. Cell sizes were measured in both isolates, allowing the definition of two main cell types based on their shape (round and elongated). On average, the filament is composed of about 40% round cells, which are present in the central region of the filament, but different combinations of the two cell types within filaments were observed and quantified. Young sporophytes grew apically, with elongated cells progressively differentiating into round cells. Secondary filaments emerged preferentially on round cells, primarily from the older central cells. Statistical analyses showed that the pattern of branching was regulated to ensure a stereotyped architecture. This description of the developmental patterning during the growth of the E. siliculosus sporophyte will serve as a base for more detailed studies of development, in this species and in brown algae in general.	[Le Bail, Aude; Charrier, Benedicte] Univ Paris 06, UPMC, CNRS, Biol Stn,UMR Vegetaux Marins & Biomol 7139, F-29682 Roscoff, France; [Billoud, Bernard] Univ Paris 06, UPMC, F-75005 Paris, France	Charrier, B (corresponding author), Univ Paris 06, UPMC, CNRS, Biol Stn,UMR Vegetaux Marins & Biomol 7139, F-29682 Roscoff, France.	charrier@sb-roscoff.fr		Billoud, Bernard/0000-0002-5140-8087; Charrier, Benedicte/0000-0001-5721-1640; Peters, Akira/0000-0001-5332-199X	Conseil Regional de BretagneRegion Bretagne	We thank D. Scornet for technical assistance for the Ectocarpus cultures, and R. Belle for advice on morphometrical analyses. A. L. B. is a fellow of the French Ministere de l'Education Nationale et de l'Enseignement Superieur et de la Recherche, and C. M. is supported by the Conseil Regional de Bretagne. We also thank the two reviewers of the manuscript, one of them being David J. Garbary.	ANDREWS M, 1984, J ECOL, V72, P873, DOI 10.2307/2259537; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Basu S, 2002, PLANT PHYSIOL, V130, P292, DOI 10.1104/pp.004747; BRADLEY PM, 1991, J PHYCOL, V27, P317, DOI 10.1111/j.0022-3646.1991.00317.x; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cho GY, 2004, J PHYCOL, V40, P921, DOI 10.1111/j.1529-8817.2004.03160.x; CORBIT JD, 1993, COMPUT GRAPH, V17, P85, DOI 10.1016/0097-8493(93)90055-E; COVE DJ, 1993, PLANT CELL, V5, P1483; De Clerck O, 2004, BOT MAR, V47, P431, DOI 10.1515/BOT.2004.060; DEREVIERS B, 2007, UNRAVELLING ALGAE PA, V75, P265; DUCREUX G, 1995, B SOC ZOOL FR, V120, P139; DUCREUX G, 1985, CRYPTOGAMIE ALGOL, V1, P35; EVANS LV, 1991, J PHYCOL, V27, P322, DOI 10.1111/j.0022-3646.1991.00322.x; FRITSCH FE, 1945, ANN BOT-LONDON, V9, P1; GARBARY D, 1988, CAN J BOT, V66, P1308, DOI 10.1139/b88-184; GARBARY DJ, 1990, PHYCOLOGIA, V29, P98, DOI 10.2216/i0031-8884-29-1-98.1; GARBARY DJ, 1992, PROGR PHYCOLOGICAL R, V8, P143; GIFFORD EM, 1983, ANNU REV PLANT PHYS, V34, P419, DOI 10.1146/annurev.pp.34.060183.002223; Harrison LG, 2001, FARADAY DISCUSS, V120, P277, DOI 10.1039/b103246c; Holloway DM, 1999, PHILOS T ROY SOC B, V354, P417, DOI 10.1098/rstb.1999.0395; Kirk DL, 2001, DEV BIOL, V238, P213, DOI 10.1006/dbio.2001.0402; KONRADHAWKINS E, 1964, PROTOPLASMA, V58, P42, DOI 10.1007/BF01252618; KONRADHAWKINS E, 1964, PROTOPLASMA, V58, P60, DOI 10.1007/BF01252619; KONRADHAWKINS E, 1968, AM J BOT, V55, P255; KORNMANN P, 1966, HELGOLAND WISS MEER, V13, P73, DOI 10.1007/BF01612657; LAMBERT C, 1995, ACTA BIOTHEOR, V43, P67, DOI 10.1007/BF00709434; Lambert C, 1999, ACTA BIOTHEOR, V47, P309, DOI 10.1023/A:1002655108602; Luck J, 1999, ACTA BIOTHEOR, V47, P329, DOI 10.1023/A:1002659209511; METRAUX JP, 1980, PLANT PHYSIOL, V65, P204, DOI 10.1104/pp.65.2.204; Momany M, 2002, CURR OPIN MICROBIOL, V5, P580, DOI 10.1016/S1369-5274(02)00368-5; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1980, PUBLIKATIONEN WIS 13, V1308, P1; Olsson T, 2003, J BIOL CHEM, V278, P44439, DOI 10.1074/jbc.M306265200; PEDERSEN M, 1968, NATURE, V218, P776, DOI 10.1038/218776a0; PEDERSEN M, 1973, PHYSIOL PLANTARUM, V28, P101, DOI 10.1111/j.1399-3054.1973.tb01158.x; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Prusinkiewicz P, 1999, AGRONOMIE, V19, P211, DOI 10.1051/agro:19990303; R Development Core Team, 2006, R LANG ENV STAT COMP; Ravanko O, 1970, NOVA HEDWIGIA, V20, P79; Rusig AM, 2001, CRYPTOGAMIE ALGOL, V22, P227, DOI 10.1016/S0181-1568(01)01066-2; SCHNEIDER CW, 1994, EUR J PHYCOL, V29, P165, DOI 10.1080/09670269400650611; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stirk WA, 2003, PLANT GROWTH REGUL, V41, P13, DOI 10.1023/A:1027376507197; Sun HG, 2004, PLANT PHYSIOL, V135, P266, DOI 10.1104/pp.103.034900; Tarakhovskaya ER, 2007, RUSS J PLANT PHYSL+, V54, P163, DOI 10.1134/S1021443707020021	50	21	21	0	7	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	OCT	2008	44	5					1269	1281		10.1111/j.1529-8817.2008.00582.x			13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	357SN	WOS:000259866800018	27041723				2021-04-07	
J	Contreras, L; Ritter, A; Dennett, G; Boehmwald, F; Guitton, N; Pineau, C; Moenne, A; Potin, P; Correa, JA				Contreras, Loretto; Ritter, Andres; Dennett, Geraldine; Boehmwald, Freddy; Guitton, Nathalie; Pineau, Charles; Moenne, Alejandra; Potin, Philippe; Correa, Juan A.			TWO-DIMENSIONAL GEL ELECTROPHORESIS ANALYSIS OF BROWN ALGAL PROTEIN EXTRACTS	JOURNAL OF PHYCOLOGY			English	Article						2-DE; algae; Phaeophyceae; protein extraction methods; proteomics	HAEMATOCOCCUS-PLUVIALIS CHLOROPHYCEAE; RECALCITRANT PLANT-TISSUES; PROTEOMIC ANALYSIS; CHLAMYDOMONAS-REINHARDTII; SOLUBILIZATION; STRESS; PHAEOPHYCEAE; INSIGHTS	High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protein extracts. The performance of the new protocol was tested against four methods available for vascular plants and a seaweed. The protocol, which includes an initial step to remove salts from the algal tissues, allowed the use of highly resolving two-dimensional gel electrophoresis (2-DE) protein analyses, providing the opportunity to unravel potentially novel physiological processes unique to this group of marine organisms.	[Contreras, Loretto; Ritter, Andres; Dennett, Geraldine; Correa, Juan A.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Adv Studies Ecol & Biodivers, Dept Ecol, Santiago, Chile; [Ritter, Andres; Potin, Philippe] Univ Paris 06, CNRS, Biol Stn, UMR 7139, Roscoff, France; [Boehmwald, Freddy; Moenne, Alejandra] Univ Santiago Chile, Dept Biol, Fac Quim & Biol, Santiago, Chile; [Guitton, Nathalie; Pineau, Charles] INSERM, High Throughput Prote Platform OUEST Genopole, UPRES JE 2459, U625, F-35042 Rennes, France	Contreras, L (corresponding author), Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Adv Studies Ecol & Biodivers, Dept Ecol, Alameda 340, Santiago, Chile.	lcontrer@puc.cl	, Protim/C-4687-2012; Contreras-Porcia, Loretto/F-9593-2016; Pineau, Charles/D-9185-2013	Contreras-Porcia, Loretto/0000-0002-6511-2244; Pineau, Charles/0000-0002-7461-5433; Ritter, Andres/0000-0001-7011-6824; POTIN, Philippe/0000-0001-7358-6282	Marine Genomics Chile (CONICYT)Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT); CONICYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT); ICA; DICYT-USACH; NoE in Marine Genomics (Europe)	This work supported by Marine Genomics Chile (CONICYT) to J. A. C. and A. M., FONDAP 1501-0001 subprogram 7 from CONICYT and ICA to J. A. C. and by DICYT-USACH to A. M. This work is part of the NoE in Marine Genomics (Europe). Protein identification was done at the high-throughput proteomic platform of OUEST-genepole (R) (Rennes, France). Our thanks go to M. Vasquez for providing several 2-DE equipment and to M. Cock for providing access to the E. siliculosus EST database. This study was conducted within the framework of the Laboratoire International Associe Dispersal and Adaptation in Marine Species (DIAMS).	Beranova-Giorgianni S, 2003, TRAC-TREND ANAL CHEM, V22, P273, DOI 10.1016/S0165-9936(03)00508-9; Carpentier SC, 2005, PROTEOMICS, V5, P2497, DOI 10.1002/pmic.200401222; Chinnasamy G, 2006, BBA-PROTEINS PROTEOM, V1764, P641, DOI 10.1016/j.bbapap.2005.10.003; Com E, 2003, MOL CELL PROTEOMICS, V2, P248, DOI 10.1074/mcp.M300010-MCP200; Contreras L, 2007, J PHYCOL, V43, P1320, DOI 10.1111/j.1529-8817.2007.00413.x; CREMER F, 1985, ANAL BIOCHEM, V147, P22, DOI 10.1016/0003-2697(85)90004-1; FLENGSRUD R, 1989, ANAL BIOCHEM, V177, P33, DOI 10.1016/0003-2697(89)90008-0; Forster B, 2006, PROTEOMICS, V6, P4309, DOI 10.1002/pmic.200500907; Gygi SP, 2000, P NATL ACAD SCI USA, V97, P9390, DOI 10.1073/pnas.160270797; Hoffmeister M, 2004, J BIOL CHEM, V279, P22422, DOI 10.1074/jbc.M400913200; Jacobs DI, 2001, PROTEOMICS, V1, P1345, DOI 10.1002/1615-9861(200111)1:11<1345::AID-PROT1345>3.0.CO;2-F; Kim YK, 2005, ECOTOXICOLOGY, V14, P589, DOI 10.1007/s10646-005-0009-5; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; Mechin V, 2003, PROTEOMICS, V3, P1299, DOI 10.1002/pmic.200300450; OFARRELL PH, 1975, J BIOL CHEM, V250, P4007; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Rabilloud T, 1996, ELECTROPHORESIS, V17, P813, DOI 10.1002/elps.1150170503; Saravanan RS, 2004, PROTEOMICS, V4, P2522, DOI 10.1002/pmic.200300789; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stauber EJ, 2003, EUKARYOT CELL, V2, P978, DOI 10.1128/EC.2.5.978-994.2003; Unlu M, 1997, ELECTROPHORESIS, V18, P2071, DOI 10.1002/elps.1150181133; Wang SB, 2004, PLANTA, V220, P17, DOI 10.1007/s00425-004-1323-5; Wang SB, 2004, PROTEOMICS, V4, P692, DOI 10.1002/pmic.200300634; Wang SB, 2003, J APPL PHYCOL, V15, P485, DOI 10.1023/B:JAPH.0000004324.88897.b2; Wang W, 2003, ELECTROPHORESIS, V24, P2369, DOI 10.1002/elps.200305500; Wong PF, 2006, J PHYCOL, V42, P113, DOI 10.1111/j.1529-8817.2006.00182.x; Yan SP, 2006, MOL CELL PROTEOMICS, V5, P484, DOI 10.1074/mcp.M500251-MCP200	27	32	33	0	24	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	OCT	2008	44	5					1315	1321		10.1111/j.1529-8817.2008.00575.x			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	357SN	WOS:000259866800023	27041728				2021-04-07	
J	de Franco, PO; Rousvoal, S; Tonon, T; Boyen, C				de Franco, Pierre-Olivier; Rousvoal, Sylvie; Tonon, Thierry; Boyen, Catherine			Whole genome survey of the glutathione transferase family in the brown algal model Ectocarpus siliculosus	MARINE GENOMICS			English	Article						Marine algae; Stress response; GST; Sigma class; Kappa class; MAPEG; Evolution; Oxylipins	S-TRANSFERASE; CRYSTAL-STRUCTURE; DROSOPHILA-MELANOGASTER; ARABIDOPSIS-THALIANA; CATALYTIC-PROPERTIES; MOLECULAR-CLONING; CHONDRUS-CRISPUS; OXIDATIVE BURST; DEATH EVOLUTION; RECENT INSIGHTS	We report here an exhaustive analysis of the glutathione transferases (GSTs) in the model brown alga Ectocarpus siliculosus using available genomic resources. A genome survey revealed the presence of twelve cytosolic GSTs, belonging to the Sigma class, two pseudogenes, one GST of the Kappa class, and three microsomal GSTs of the MGST3 family of membrane associated protein involved in eicosanoid and glutathione metabolism. Gene structure and phylogenetic analyses demonstrated the partition of the Sigma GSTs into two clusters which have probably evolved by duplication events. Gene expression profiling was conducted after the addition of high concentrations of chemicals, such as H2O2, herbicides, heavy metals, as well as fatty acid derivatives, in order to induce stress conditions and to monitor early response mechanisms. The results of these experiments suggested that E. siliculosus GST genes are recruited in different and specific conditions. In addition, heterologous expression in yeast of two E. siliculosus microsomal GST showed that these enzymes feature peroxidase rather than transferase activity. The potential involvement of E. siliculosus GST in the metabolism of oxygenated polyunsaturated fatty acids is discussed. (C) 2009 Elsevier B.V. All rights reserved.	[de Franco, Pierre-Olivier; Rousvoal, Sylvie; Tonon, Thierry; Boyen, Catherine] CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France; [de Franco, Pierre-Olivier; Rousvoal, Sylvie; Tonon, Thierry; Boyen, Catherine] Univ Paris 06, UPMC, Biol Stn, UMR Vegetaux Marins & Biomol 7139, F-29682 Roscoff, France	Tonon, T (corresponding author), CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, Pl Georges Teissier,BP 74, F-29682 Roscoff, France.	tonon@sb-roscoff.fr	Tonon, Thierry/A-3214-2009	Tonon, Thierry/0000-0002-1454-6018	French Ministere de l'Enseignement Superieur et de la Recherche; European CommissionEuropean CommissionEuropean Commission Joint Research Centre [GOCE-CT-2004-505403]	P.-O. de F. was supported by the French Ministere de l'Enseignement Superieur et de la Recherche. We are grateful to Dr J.M. Cock for providing access to the E siliculosus genomic resources, and to J. Collen for critical reading of the manuscript. Part of this work was performed within the framework of the 'Marine Genomics Europe' NoE (Network of Excellence) (European Commission contract No. GOCE-CT-2004-505403).	Agianian B, 2003, J MOL BIOL, V326, P151, DOI 10.1016/S0022-2836(02)01327-X; Armstrong RN, 1997, CHEM RES TOXICOL, V10, P2, DOI 10.1021/tx960072x; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; BARTLING D, 1993, EUR J BIOCHEM, V216, P579, DOI 10.1111/j.1432-1033.1993.tb18177.x; Bartsch I, 2008, EUR J PHYCOL, V43, P1, DOI 10.1080/09670260701711376; Board PG, 1997, BIOCHEM J, V328, P929; Board PG, 2000, J BIOL CHEM, V275, P24798, DOI 10.1074/jbc.M001706200; BOOTH J, 1961, BIOCHEM J, V79, P516, DOI 10.1042/bj0790516; Bouarab K, 2004, PLANT PHYSIOL, V135, P1838, DOI 10.1104/pp.103.037622; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; Bresell A, 2005, FEBS J, V272, P1688, DOI 10.1111/j.1742-4658.2005.04596.x; Cairrao E, 2004, AQUAT TOXICOL, V70, P277, DOI 10.1016/j.aquatox.2004.09.005; Cedergreen N, 2005, PEST MANAG SCI, V61, P1152, DOI 10.1002/ps.1117; Cha CJ, 2002, BIOCHEM J, V368, P589, DOI 10.1042/BJ20020400; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cho SG, 2001, J BIOL CHEM, V276, P12749, DOI 10.1074/jbc.M005561200; Coleman JOD, 1997, PLANT CELL ENVIRON, V20, P449, DOI 10.1046/j.1365-3040.1997.d01-93.x; Collen J, 2006, J PHYCOL, V42, P104, DOI 10.1111/j.1529-8817.2006.00171.x; Collen J, 1999, J PHYCOL, V35, P62, DOI 10.1046/j.1529-8817.1999.3510062.x; COMBES B, 1961, J CLIN INVEST, V40, P981, DOI 10.1172/JCI104337; Correa JA, 1999, J APPL PHYCOL, V11, P57, DOI 10.1023/A:1008027610826; COSSE A, 2007, ADV BOT RES, V46, P222; Dereeper A, 2008, NUCLEIC ACIDS RES, V36, pW465, DOI 10.1093/nar/gkn180; Ding YC, 2003, BMC GENOMICS, V4, DOI 10.1186/1471-2164-4-35; Dixon DP, 2002, GENOME BIOL, V3, DOI 10.1186/gb-2002-3-3-reviews3004; Dring MJ, 2006, ADV BOT RES, V43, P175, DOI 10.1016/S0065-2296(05)43004-9; Edwards R, 2000, TRENDS PLANT SCI, V5, P193, DOI 10.1016/S1360-1385(00)01601-0; Fernandez-Canon JM, 1998, J BIOL CHEM, V273, P329, DOI 10.1074/jbc.273.1.329; Frova C, 2006, BIOMOL ENG, V23, P149, DOI 10.1016/j.bioeng.2006.05.020; Funk CD, 2001, SCIENCE, V294, P1871, DOI 10.1126/science.294.5548.1871; Gaquerel E, 2007, BBA-MOL CELL BIOL L, V1771, P565, DOI 10.1016/j.bbalip.2007.02.007; Gerwick WH, 1999, ADV EXP MED BIOL, V447, P211; Hall TA.., 1999, NUCL ACIDS S SERIES, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Hayes JD, 2005, ANNU REV PHARMACOL, V45, P51, DOI 10.1146/annurev.pharmtox.45.120403.095857; Herve C, 2008, BIOCHEM J, V412, P535, DOI 10.1042/BJ20071464; ISHIKAWA T, 1992, TRENDS BIOCHEM SCI, V17, P463, DOI 10.1016/0968-0004(92)90489-V; Jakobsson PJ, 1997, J BIOL CHEM, V272, P22934, DOI 10.1074/jbc.272.36.22934; Jakobsson PJ, 1999, PROTEIN SCI, V8, P689, DOI 10.1110/ps.8.3.689; Jowsey IR, 2001, BIOCHEM J, V359, P507, DOI 10.1042/0264-6021:3590507; Kanaoka Y, 2000, EUR J BIOCHEM, V267, P3315, DOI 10.1046/j.1432-1327.2000.01362.x; Kanaoka Y, 1997, CELL, V90, P1085, DOI 10.1016/S0092-8674(00)80374-8; Konishi T, 2005, BIOCHEM J, V388, P299, DOI 10.1042/BJ20041578; Kupper FC, 2006, J EXP BOT, V57, P1991, DOI 10.1093/jxb/erj146; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; Le Bail A, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-75; Li GQ, 2004, BRIEF BIOINFORM, V5, P378, DOI 10.1093/bib/5.4.378; Marrs KA, 1996, ANNU REV PLANT PHYS, V47, P127, DOI 10.1146/annurev.arplant.47.1.127; MEYER DJ, 1996, BIOCHEM J, P223; Monaco R, 1999, J PROTEIN CHEM, V18, P859, DOI 10.1023/A:1020679229110; Morel F, 2004, J BIOL CHEM, V279, P16246, DOI 10.1074/jbc.M313357200; Nebert Daniel W., 2004, Human Genomics, V1, P460; Nei M, 2005, ANNU REV GENET, V39, P121, DOI 10.1146/annurev.genet.39.073003.112240; Nei M, 2000, P NATL ACAD SCI USA, V97, P10866, DOI 10.1073/pnas.97.20.10866; Pearson WR, 2005, METHOD ENZYMOL, V401, P186, DOI 10.1016/S0076-6879(05)01012-8; Pemble SE, 1996, BIOCHEM J, V319, P749, DOI 10.1042/bj3190749; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Pflugmacher S, 2000, PHYTOCHEMISTRY, V54, P267, DOI 10.1016/S0031-9422(00)00116-3; Pieniazek D, 2004, PESTIC BIOCHEM PHYS, V79, P58, DOI 10.1016/j.pestbp.2004.03.003; Ranson H, 2005, METHOD ENZYMOL, V401, P226, DOI 10.1016/S0076-6879(05)01014-1; Ritter A, 2008, NEW PHYTOL, V180, P809, DOI 10.1111/j.1469-8137.2008.02626.x; Roeder V, 2005, J PHYCOL, V41, P1227, DOI 10.1111/j.1529-8817.2005.00150.x; Rowley AF, 2005, J EXP BIOL, V208, P3, DOI 10.1242/jeb.01275; Sawicki R, 2003, BIOCHEM J, V370, P661, DOI 10.1042/BJ20021287; Schmidt-Krey I, 2000, EMBO J, V19, P6311, DOI 10.1093/emboj/19.23.6311; Schuller DJ, 2005, PROTEINS, V61, P1024, DOI 10.1002/prot.20649; Sheehan D, 2001, BIOCHEM J, V360, P1, DOI 10.1042/0264-6021:3600001; Singh SP, 2001, EUR J BIOCHEM, V268, P2912, DOI 10.1046/j.1432-1327.2001.02179.x; Soranzo N, 2004, MOL GENET GENOMICS, V271, P511, DOI 10.1007/s00438-004-1006-8; Stanley D, 2006, ANNU REV ENTOMOL, V51, P25, DOI 10.1146/annurev.ento.51.110104.151021; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Thom R, 2001, J MOL BIOL, V308, P949, DOI 10.1006/jmbi.2001.4638; Thomson AM, 1998, BIOCHEM J, V333, P317, DOI 10.1042/bj3330317; Tu CPD, 2005, METHOD ENZYMOL, V401, P204, DOI 10.1016/S0076-6879(05)01013-X; Valles SM, 2006, ARCH INSECT BIOCHEM, V61, P239, DOI 10.1002/arch.20116; Wagner U, 2002, PLANT MOL BIOL, V49, P515, DOI 10.1023/A:1015557300450; Yamamoto K, 2007, J APPL ENTOMOL, V131, P466, DOI 10.1111/j.1439-0418.2007.01150.x; Zhang JZ, 2003, TRENDS ECOL EVOL, V18, P292, DOI 10.1016/S0169-5347(03)00033-8	78	16	16	0	7	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1874-7787	1876-7478		MAR GENOM	Mar. Genom.	SEP-DEC	2008	1	3-4					135	148		10.1016/j.margen.2009.01.003			14	Genetics & Heredity; Marine & Freshwater Biology	Genetics & Heredity; Marine & Freshwater Biology	V10OH	WOS:000207472800009	21798165				2021-04-07	
J	Fricke, A; Molis, M; Wiencke, C; Valdivia, N; Chapman, AS				Fricke, Anna; Molis, Markus; Wiencke, Christian; Valdivia, Nelson; Chapman, Annelise S.			Natural succession of macroalgal-dominated epibenthic assemblages at different water depths and after transplantation from deep to shallow water on Spitsbergen	POLAR BIOLOGY			English	Article						Arctic; diversity; macroalgal; recruitment; sublittoral; succession; hard bottom; community; transplantation	SOLAR ULTRAVIOLET-RADIATION; UV-ABSORBING COMPOUNDS; ARCTIC FJORD; SETTLEMENT CUES; ECOPHYSIOLOGICAL PATTERNS; MACROBENTHIC COMMUNITY; BALANUS-AMPHITRITE; SEASONAL-VARIATION; OZONE DEPLETION; HIGH LIGHT	In the current study, we investigated the primary succession of seaweeds over different time periods at different water depths. Furthermore, we followed the succession of field-grown benthic communities of different successional age, developing on ceramic tiles, prior to and after transplantation from 8 to 0.5 m water depth. The transplantation simulated changes associated with the break up of sea-ice cover, e.g. light regime or wave exposure. For this purpose, we transplanted 12 and 21-month old communities, grown at 8 m water depth, together with a set of sterile tiles, onto rafts, floating in 0.5 m water depth. Our results describe for the first time the succession of macroalgal communities in the Arctic and give important insights into the effect of disturbance of differently aged communities. The primary succession at 0.5 m water depth was mainly driven by Bacillariophyta and filamentous green algae like Urospora sp. and Ulothrix implexa. Twelve-month old communities at 8 m water depth are dominated by members of the Ectocarpales (Phaeophyceae), like Pylaiella littoralis, P. varia, and Ectocarpus siliculosus and the green alga U. implexa, whereas the 21-month old community showed a higher cover of the green algal class Ulvophyceae and sessile invertebrates. After transplantation to near surface conditions, species composition of the communities changed, but this effect was differently strong between communities of different age.	[Fricke, Anna] Ctr Trop Marine Ecol, D-28359 Bremen, Germany; [Fricke, Anna; Wiencke, Christian] Alfred Wegener Inst Polar & Marine Res, Sect Seaweed Biol, D-27570 Bremerhaven, Germany; [Molis, Markus; Valdivia, Nelson] Alfred Wegener Inst Polar & Marine Res, Marine Stn, Sect Seaweed Biol, D-27498 Helgoland, Germany; [Chapman, Annelise S.] Dalhousie Univ, Dept Biol, Halifax, NS BH3 4J1, Canada	Fricke, A (corresponding author), Ctr Trop Marine Ecol, Fahrenheitstr 6, D-28359 Bremen, Germany.	anna.fricke@zmt-bremen.de	Valdivia, Nelson A/C-3613-2009	Valdivia, Nelson A/0000-0002-5394-2072; Fricke, Anna/0000-0003-1188-0114	AWIHelmholtz Association	This work was part of the diploma thesis of the first author and has been carried out at the Ny Alesund International Research and Monitory Facility. The authors thank the German scientific diving crew under the leadership of Max Schwanitz: Claudia Daniel, Peter Leopold, and Michael Tessmann for assistance in the field, as well as the Koldewey Station team Rainer Vockenroth, Kai Marholdt, and Cedric Couret for support. Thank to Betti Saier, for assistance in the sampling, conducted in 2005. Thank to Ruth Muller for assistance in measuring the environmental data during the experimental time. Thanks for help in identification questions to Mara Schmiing, Jana Wolfel, Ulf Karsten, and Margaret Clayton. We gratefully acknowledge financial support by the AWI.	Aguilera J, 2002, MAR BIOL, V140, P1087, DOI 10.1007/s00227-002-0792-y; Albrecht AS, 1998, J EXP MAR BIOL ECOL, V229, P85, DOI 10.1016/S0022-0981(98)00044-6; Barnes DKA, 2007, PHILOS T R SOC B, V362, P11, DOI 10.1098/rstb.2006.1951; Beuchel F, 2006, J MARINE SYST, V63, P35, DOI 10.1016/j.jmarsys.2006.05.002; Bischof K, 1999, PLANT BIOLOGY, V1, P435, DOI 10.1055/s-2007-978537; Bischof K, 2002, MAR BIOL, V140, P1097, DOI 10.1007/s00227-002-0795-8; Bischof K, 1998, MAR BIOL, V131, P597, DOI 10.1007/s002270050351; CAMPANA G, 2008, REP POLAR MAR RES, V571, P302; Clarke KR, 2001, MAR ECOL PROG SER, V216, P265, DOI 10.3354/meps216265; Clarke KR., 2006, PRIMER V6 USER MANUA; Coelho Susana M., 2000, Journal of Aquatic Ecosystem Stress and Recovery, V7, P317, DOI 10.1023/A:1009916129009; Connell J.H., 1985, P125; CONNELL JH, 1977, AM NAT, V111, P1119, DOI 10.1086/283241; Dobretsov SV, 2005, MAR ECOL PROG SER, V290, P55, DOI 10.3354/meps290055; DUNTON KH, 1982, ARCTIC, V35, P465; FALKOWSKI PG, 1991, J PHYCOL, V27, P8, DOI 10.1111/j.0022-3646.1991.00008.x; FARRELL TM, 1991, ECOL MONOGR, V61, P95, DOI 10.2307/1943001; Hanelt D, 1997, J PHOTOCH PHOTOBIO B, V38, P40, DOI 10.1016/S1011-1344(96)07415-5; Hanelt D, 2001, MAR BIOL, V138, P649, DOI 10.1007/s002270000481; HASSOL SJ, 2005, ACIA ARKTIS KLIMAREP; Hop H, 2002, POLAR RES, V21, P167, DOI 10.1111/j.1751-8369.2002.tb00073.x; Hung OS, 2007, MAR ECOL PROG SER, V333, P229, DOI 10.3354/meps333229; Karsten U, 1998, AQUAT MICROB ECOL, V16, P37, DOI 10.3354/ame016037; Karsten U, 2001, OECOLOGIA, V127, P11, DOI 10.1007/s004420000553; Karsten U, 2006, POLAR BIOL, V29, P476, DOI 10.1007/s00300-005-0078-1; KERR JB, 1993, SCIENCE, V262, P1032, DOI 10.1126/science.262.5136.1032; Khandeparker L, 2006, FEMS MICROBIOL ECOL, V58, P425, DOI 10.1111/j.1574-6941.2006.00177.x; Kirk J.T.O., 1994, LIGHT PHOTOSYNTHESIS, V2nd ed., P509; KIRST GO, 1995, J PHYCOL, V31, P181, DOI 10.1111/j.0022-3646.1995.00181.x; Lam C, 2005, MAR ECOL PROG SER, V294, P109, DOI 10.3354/meps294109; Lippert H, 2001, POLAR BIOL, V24, P512; LITTLER MM, 1980, BOT MAR, V22, P161; Lotze HK, 2002, MAR ECOL PROG SER, V243, P57, DOI 10.3354/meps243057; LUDER UH, 2008, POLAR BIOL UNPUB; McMahon KW, 2006, MAR ECOL PROG SER, V310, P1, DOI 10.3354/meps310001; Molis M, 2004, J EXP MAR BIOL ECOL, V302, P51, DOI 10.1016/j.jembe.2003.10.003; Newell RC, 1998, OCEANOGR MAR BIOL<D>, V36, P127; PAWLIK JR, 1992, OCEANOGR MAR BIOL, V30, P273; Piepenburg D, 2005, POLAR BIOL, V28, P733, DOI 10.1007/s00300-005-0013-5; RAIMONDI PT, 1988, ECOLOGY, V69, P400, DOI 10.2307/1940438; Roleda MY, 2006, J EXP BOT, V57, P3847, DOI 10.1093/jxb/erl154; Roleda MY, 2006, PHOTOSYNTH RES, V88, P311, DOI 10.1007/s1120-006-9055-y; ROLEDA MY, 2006, EFFECTS ULTRAVIOLET, P176; SOUSA WP, 1984, ANNU REV ECOL SYST, V15, P353, DOI 10.1146/annurev.es.15.110184.002033; Svendsen H, 2002, POLAR RES, V21, P133, DOI 10.1111/j.1751-8369.2002.tb00072.x; Underwood AJ., 1997, EXPT ECOLOGY THEIR L; Vinebrooke RD, 1999, ECOLOGY, V80, P223; Vinogradova K. L., 1995, Botanicheskii Zhurnal (St. Petersburg), V80, P50; Wahl M, 2004, GLOBAL CHANGE BIOL, V10, P1962, DOI 10.1111/j.1365-2486.2004.00872.x; WESLAWSKI JM, 1993, POLAR BIOL, V13, P73; WETHEY DS, 1986, B MAR SCI, V39, P393; Weykam G, 1996, J EXP MAR BIOL ECOL, V204, P1, DOI 10.1016/0022-0981(96)02576-2; Weykam G, 1997, PHYCOLOGIA, V36, P395, DOI 10.2216/i0031-8884-36-5-395.1; Wiencke C., 2007, Reviews in Environmental Science and Bio/Technology, V6, P95, DOI 10.1007/s11157-006-9106-z; WIENCKE C, 2004, POLARFORSCH MEERESFO, V492, P55; WULFF A, 2008, REP POLAR MAR RES, V571, P263; Zacher K, 2007, GLOBAL CHANGE BIOL, V13, P1201, DOI 10.1111/j.1365-2486.2007.01349.x	57	15	15	0	22	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0722-4060			POLAR BIOL	Polar Biol.	SEP	2008	31	10					1191	1203		10.1007/s00300-008-0458-4			13	Biodiversity Conservation; Ecology	Biodiversity & Conservation; Environmental Sciences & Ecology	340HB	WOS:000258635600005					2021-04-07	
J	Le Bail, A; Dittami, SM; de Franco, PO; Rousvoal, S; Cock, MJ; Tonon, T; Charrier, B				Le Bail, Aude; Dittami, Simon M.; de Franco, Pierre-Olivier; Rousvoal, Sylvie; Cock, Mark J.; Tonon, Thierry; Charrier, Benedicte			Normalisation genes for expression analyses in the brown alga model Ectocarpus siliculosus	BMC MOLECULAR BIOLOGY			English	Article							TIME RT-PCR; RELIABLE REFERENCE GENES; HOUSEKEEPING GENES; ENDOGENOUS CONTROL; VALIDATION; SELECTION; IDENTIFICATION; IMPACT; CELLS; SKIN	Background: Brown algae are plant multi-cellular organisms occupying most of the world coasts and are essential actors in the constitution of ecological niches at the shoreline. Ectocarpus siliculosus is an emerging model for brown algal research. Its genome has been sequenced, and several tools are being developed to perform analyses at different levels of cell organization, including transcriptomic expression analyses. Several topics, including physiological responses to osmotic stress and to exposure to contaminants and solvents are being studied in order to better understand the adaptive capacity of brown algae to pollution and environmental changes. A series of genes that can be used to normalise expression analyses is required for these studies. Results: We monitored the expression of 13 genes under 21 different culture conditions. These included genes encoding proteins and factors involved in protein translation (ribosomal protein 26S, EF1alpha, IF2A, IF4E) and protein degradation (ubiquitin, ubiquitin conjugating enzyme) or folding (cyclophilin), and proteins involved in both the structure of the cytoskeleton (tubulin alpha, actin, actin-related proteins) and its trafficking function (dynein), as well as a protein implicated in carbon metabolism (glucose 6-phosphate dehydrogenase). The stability of their expression level was assessed using the Ct range, and by applying both the geNorm and the Normfinder principles of calculation. Conclusion: Comparisons of the data obtained with the three methods of calculation indicated that EF1alpha (EF1a) was the best reference gene for normalisation. The normalisation factor should be calculated with at least two genes, alpha tubulin, ubiquitin-conjugating enzyme or actin-related proteins being good partners of EF1a. Our results exclude actin as a good normalisation gene, and, in this, are in agreement with previous studies in other organisms.	[Le Bail, Aude; Dittami, Simon M.; de Franco, Pierre-Olivier; Rousvoal, Sylvie; Cock, Mark J.; Tonon, Thierry; Charrier, Benedicte] UPMC Univ Paris 6, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France; [Le Bail, Aude; Dittami, Simon M.; de Franco, Pierre-Olivier; Rousvoal, Sylvie; Cock, Mark J.; Tonon, Thierry; Charrier, Benedicte] CNRS, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France	Charrier, B (corresponding author), UPMC Univ Paris 6, UMR Vegetaux Marins & Biomol 7139, Biol Stn, F-29682 Roscoff, France.	lebail@sb-roscoff.fr; dittami@sb-roscoff.fr; defranco@sb-roscoff.fr; rousvoal@sb-roscoff.fr; cock@sb-roscoff.fr; tonon@sb-roscoff.fr; charrier@sb-roscoff.fr	Tonon, Thierry/A-3214-2009; Dittami, Simon/E-8354-2011	Tonon, Thierry/0000-0002-1454-6018; Dittami, Simon/0000-0001-7987-7523; Charrier, Benedicte/0000-0001-5721-1640; Cock, J. Mark/0000-0002-2650-0383	European community's Sixth Framework Programme [2005-020737]	We thank Julia Morales (Station Biologique de Roscoff) for her help concerning the identification of the IF2A and EFI4 genes, and Bernard Billoud (Atelier de Bioinformatique de l'Universite Pierre et Marie Curie Paris-6) for his assistance with statistical analyses. ALB and P-O dF are granted by the French Ministere de l'Enseignement Superieur et de la Recherche. SD has received funding from the European community's Sixth Framework Programme (ESTeam n contract MESTCT 2005-020737).	Andersen CL, 2004, CANCER RES, V64, P5245, DOI 10.1158/0008-5472.CAN-04-0496; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Blee E, 2002, TRENDS PLANT SCI, V7, P315, DOI 10.1016/S1360-1385(02)02290-2; Bogaert L, 2006, BMC BIOTECHNOL, V6, DOI 10.1186/1472-6750-6-24; Brunner Amy M., 2004, BMC Plant Biology, V4, P14, DOI 10.1186/1471-2229-4-14; Bustin SA, 2005, J MOL ENDOCRINOL, V34, P597, DOI 10.1677/jme.1.01755; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; COSSE A, 2007, ADV BOT RES, V46, P222; Czechowski T, 2005, PLANT PHYSIOL, V139, P5, DOI 10.1104/pp.105.063743; Dring MJ, 2006, ADV BOT RES, V43, P175, DOI 10.1016/S0065-2296(05)43004-9; Funk CD, 2001, SCIENCE, V294, P1871, DOI 10.1126/science.294.5548.1871; Gachon C, 2004, J EXP BOT, V55, P1445, DOI 10.1093/jxb/erh181; Giacomazzi S, 2004, CHEMOSPHERE, V56, P1021, DOI 10.1016/j.chemosphere.2004.04.061; Goossens K, 2007, BMC DEV BIOL, V7, DOI 10.1186/1471-213X-7-64; Graymore M, 2001, ENVIRON INT, V26, P483, DOI 10.1016/S0160-4120(01)00031-9; Hibbeler S, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-18; Hoogewijs D, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-9; Ingerslev HC, 2006, MOL IMMUNOL, V43, P1194, DOI 10.1016/j.molimm.2005.07.009; Jain M, 2006, BIOCHEM BIOPH RES CO, V345, P646, DOI 10.1016/j.bbrc.2006.04.140; Kuijk EW, 2007, BMC DEV BIOL, V7, DOI 10.1186/1471-213X-7-58; McNeill RE, 2007, BMC MOL BIOL, V8, DOI 10.1186/1471-2199-8-107; Nicot N, 2005, J EXP BOT, V56, P2907, DOI 10.1093/jxb/eri285; Perez S, 2007, BMC MOL BIOL, V8, DOI 10.1186/1471-2199-8-114; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2008, DEVELOPMENT, V135, P1503, DOI 10.1242/dev.016303; Pfaffl MW, 2004, BIOTECHNOL LETT, V26, P509, DOI 10.1023/B:BILE.0000019559.84305.47; Pombo-Suarez M, 2008, BMC MOL BIOL, V9, DOI 10.1186/1471-2199-9-17; Reid KE, 2006, BMC PLANT BIOL, V6, DOI 10.1186/1471-2229-6-27; Spinsanti G, 2006, BMC MOL BIOL, V7, DOI 10.1186/1471-2199-7-32; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Takle GW, 2007, BMC PLANT BIOL, V7, DOI 10.1186/1471-2229-7-50; Vandesompele J, 2002, GENOME BIOL, V3, DOI 10.1186/gb-2002-3-7-research0034; Watson S, 2007, VIROL J, V4, DOI 10.1186/1743-422X-4-130	34	75	76	0	23	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2199			BMC MOL BIOL	BMC Mol. Biol.	AUG 18	2008	9								75	10.1186/1471-2199-9-75			9	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	349YS	WOS:000259319800001	18710525	Other Gold, Green Published			2021-04-07	
J	Fan, XL; Wang, GC; Li, DM; Xu, P; Shen, SD				Fan Xiaolei; Wang Guangce; Li Demao; Xu Pu; Shen Songdong			Study on early-stage development of conchospore in Porphyra yezoensis Ueda	AQUACULTURE			English	Article						attachment; cell wall formation; conchospore; light effects; Porphyra yezoensis	FOULING ALGA ECTOCARPUS; REPRODUCTIVE STAGES; RED ALGAE; RHODOPHYTA; ULTRASTRUCTURE; LIGHT; GROWTH; LEUCOSTICTA; BANGIALES; ACROCHAETIUM	Porphyra yezoensis Ueda (Rhodophyta) is a seaweed of economic importance with a typical dimorphic life cycle consisting of a leafy gametophyte and a filamentous sporophyte. Recently, it has been recognized as a model system for fundamental and applied studies in marine biological sciences. Conchospore, a major spore linking the two distinct multicellular phases in the life cycle, is most widely used in the breeding of P. yezoensis. In this paper, the early-stage development of conchospore, including the attachment and the cell wall formation, was studied with fluorescent reagents staining and Scanning Electron Microscopy detection. Results displayed: (I) the cell wall began to be generated after culturing for 4 h in the attached conchospores; (2) the initially released conchospores were plastids with some filmy, amorphous substance on the surface, and they attached to the fibers firmly via the actively secreted mucilaginous substances after their touch to the fibers; (3) cellulase and pectolase prohibited the attachment of conchospores in the different ways; and (4) only attached conchospores generated cell walls and developed normally, while the suspending ones could not. It indicated that the cellulose played crucial roles in the permanent attachment as the pectin did in the initial attachment. The conchospore attachment seemed to trigger the cell wall formation and the further development. Affects of light on the development of conchospores were also discussed. The results showed that high intensity (200 mu mol.m(-2).s(-1)) and long-wave (>= 580 nm) light facilitated the division rate of conchospores. (C) 2008 Elsevier B.V. All rights reserved.	[Fan Xiaolei; Wang Guangce; Li Demao] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Fan Xiaolei; Li Demao] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Wang Guangce] Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China; [Xu Pu] Changshu Inst Technol, Changshu 215500, Peoples R China; [Shen Songdong] Soochow Univ, Sch Life Sci, Suzhou 215006, Peoples R China	Wang, GC (corresponding author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.	gcwang@ms.qdio.ac.cn	Shen, Songdong/G-9385-2011	Shen, Songdong/0000-0002-5731-1681			Abe J, 2000, DNA RES, V7, P309, DOI 10.1093/dnares/7.5.309; Aguilera J, 1997, EUR J PHYCOL, V32, P417, DOI 10.1017/S096702629700142X; Asamizu E, 2003, J PHYCOL, V39, P923, DOI 10.1046/j.1529-8817.2003.03003.x; BAKER JRJ, 1973, PROTOPLASMA, V77, P181, DOI 10.1007/BF01276756; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BONEY A D, 1969, Nova Hedwigia, V18, P341; BONEY AD, 1963, J MAR BIOL ASSOC UK, V43, P319, DOI 10.1017/S0025315400000345; BONEY AD, 1966, BIOL MARINE ALGAE; Bouzon ZL, 2005, PHYCOLOGIA, V44, P409, DOI 10.2216/0031-8884(2005)44[409:UOTGIT]2.0.CO;2; BRATEN T, 1975, PROTOPLASMA, V84, P161, DOI 10.1007/BF02075951; BROWN TE, 1968, J PHYCOL, V4, P38, DOI 10.1111/j.1529-8817.1968.tb04675.x; CALLOW ME, 1973, PROTOPLASMA, V80, P15; CHAMBERL.AH, 1973, PROTOPLASMA, V76, P139, DOI 10.1007/BF01280694; CHAMBERLAIN AHL, 1981, P 8 INT SEAW S, P539; CHARNOFSKY K, 1982, J PHYCOL, V18, P417, DOI 10.1111/j.0022-3646.1982.00417.x; CHEN YC, 1995, BOT MAR, V38, P393, DOI 10.1515/botm.1995.38.1-6.393; CHRISTIE AO, 1970, ANN BOT-LONDON, V34, P467, DOI 10.1093/oxfordjournals.aob.a084383; COLE K, 1975, Phycologia, V14, P239, DOI 10.2216/i0031-8884-14-4-239.1; COLE K, 1972, SOME ELECT MICROSCOP, P157; DIXON PS, 1970, ANN NY ACAD SCI, V175, P764, DOI 10.1111/j.1749-6632.1970.tb45191.x; Dixon PS, 1973, BIOL RHODOPHYTA; DRING MJ, 1967, NATURE, V215, P1411, DOI 10.1038/2151411a0; FLETCHER RL, 1992, BRIT PHYCOL J, V27, P303, DOI 10.1080/00071619200650281; KITADE Y, 1999, FISH GENET BREED SCI, V28, P27; Kitade Yukihiro, 1998, Phycological Research, V46, P17, DOI 10.1111/j.1440-1835.1998.tb00092.x; Korbee N, 2005, J PHOTOCH PHOTOBIO B, V80, P71, DOI 10.1016/j.jphotobiol.2005.03.002; LARPENT JP, 1978, Z PFLANZENPHYSIOL, V88, P363, DOI 10.1016/S0044-328X(78)80140-8; LEE RE, 1970, J PHYCOL, V6, P22, DOI 10.1111/j.0022-3646.1970.00022.x; Li SY, 1980, OCEANOL LIMNOL SIN, V11, P370; LIN HP, 1977, BOT MAR, V20, P339, DOI 10.1515/botm.1977.20.6.339; [骆其君 Luo Qijun], 2002, [水产学报, Journal of Fisheries of China], V26, P477; MIRIAM PF, 1990, J PHYCOL, V20, P674; NATHALIE K, 2005, J PHOTOCH PHOTOBIO B, V80, P71; Perez-Rodriguez E, 2003, J EXP BOT, V54, P1093, DOI 10.1093/jxb/erg111; PUESCHEL CM, 1979, J PHYCOL, V15, P409; PUESCHEL CM, 1985, J PHYCOL, V21, P146; SHIVJI MS, 1991, CURR GENET, V19, P49, DOI 10.1007/BF00362087; TAKU M, 2003, MAR BIOTECHNOL, V5, P194; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; VANDERVELDE HH, 1978, PLANT SCI LETT, V11, P145, DOI 10.1016/0304-4211(78)90117-7; WEST J.A., 1972, P INT SEAWEED S, V7, P377; Yamazaki A, 1998, J PHYCOL, V34, P1082, DOI 10.1046/j.1529-8817.1998.341082.x; Yoshida T, 1997, NAT HIST RES, V3, P5; ZHU JY, 1980, J FISH CHINA, V4, P135	44	13	16	1	22	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	0044-8486	1873-5622		AQUACULTURE	Aquaculture	JUN 10	2008	278	1-4					143	149		10.1016/j.aquaculture.2008.02.037			7	Fisheries; Marine & Freshwater Biology	Fisheries; Marine & Freshwater Biology	317FH	WOS:000257004800021					2021-04-07	
J	Peters, AF; Scornet, D; Ratin, M; Charrier, B; Monnier, A; Merrien, Y; Corre, E; Coelho, SM; Cock, JM				Peters, Akira F.; Scornet, Delphine; Ratin, Morgane; Charrier, Benedicte; Monnier, Annabelle; Merrien, Yves; Corre, Erwan; Coelho, Susana M.; Cock, J. Mark			Life-cycle-generation-specific developmental processes are modified in the immediate upright mutant of the brown alga Ectocarpus siliculosus	DEVELOPMENT			English	Article						brown algae; Ectocarpus siliculosus; immediate upright; initial-cell division; life cycle; phaeophyceae	ASYMMETRIC CELL-DIVISION; POLARITY; FUCUS; EVOLUTION; PHAEOPHYCEAE; POLARIZATION	Development of the sporophyte and gametophyte generations of the brown alga E. siliculosus involves two different patterns of early development, which begin with either a symmetric or an asymmetric division of the initial cell, respectively. A mutant, immediate upright (imm), was isolated that exhibited several characteristics typical of the gametophyte during the early development of the sporophyte generation. Genetic analyses showed that imm is a recessive, single-locus Mendelian factor and analysis of gene expression in this mutant indicated that the regulation of a number of life-cycle-regulated genes is specifically modified in imm mutant sporophytes. Thus, IMM appears to be a regulatory locus that controls part of the sporophyte-specific developmental programme, the mutant exhibiting partial homeotic conversion of the sporophyte into the gametophyte, a phenomenon that has not been described previously.	[Peters, Akira F.; Scornet, Delphine; Ratin, Morgane; Charrier, Benedicte; Merrien, Yves; Coelho, Susana M.; Cock, J. Mark] Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, F-29682 Roscoff, France; [Peters, Akira F.; Scornet, Delphine; Ratin, Morgane; Charrier, Benedicte; Merrien, Yves; Coelho, Susana M.; Cock, J. Mark] CNRS, UMR 7139, Lab Int Associe Dispersal & Adaptat Marine Specie, Stn Biol Roscoff, F-29682 Roscoff, France; [Monnier, Annabelle] Univ Rennes 1, Fac Med, OUEST Genopole, F-35043 Rennes, France; [Corre, Erwan] Comp & Genom Resource Ctr, FR 2424, Stn Biol Roscoff, F-29682 Roscoff, France	Cock, JM (corresponding author), Univ Paris 06, Marine Plants & Biomol Lab, UMR 7139, Stn Biol Roscoff, Pl Georges Teissier,BP74, F-29682 Roscoff, France.	cock@sb-roscoff.fr	corre, erwan/O-4669-2019; Coelho, Susana/ABH-8166-2020	corre, erwan/0000-0001-6354-2278; Charrier, Benedicte/0000-0001-5721-1640; Peters, Akira/0000-0001-5332-199X; Cock, J. Mark/0000-0002-2650-0383			APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Berleth T, 2002, ARABIDOPSIS BOOK; Brownlee C, 1998, SEMIN CELL DEV BIOL, V9, P179, DOI 10.1006/scdb.1997.0212; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Corellou F, 2005, J CELL SCI, V118, P2723, DOI 10.1242/jcs.02353; de Reviers B., 2002, BIOL PHYLOGENIE ALGU, V1; DEREVIERS B, 2003, BIOL PHYLOGENIE ALGU, V2; FLETCHER R.L., 1987, SEAWEEDS BRIT ISLES, V3; Fowler JE, 2004, PLANTA, V219, P856, DOI 10.1007/s00425-004-1283-9; HORVITZ HR, 1992, CELL, V68, P237, DOI 10.1016/0092-8674(92)90468-R; Hughes JS, 1999, AM NAT, V154, P306, DOI 10.1086/303241; Huynh JR, 2004, CURR BIOL, V14, pR438, DOI 10.1016/j.cub.2004.05.040; Kawai H., 2005, ALGAL CULTURING TECH, DOI DOI 10.1007/S13398-014-0173-7.2; KORNMANN P., 1956, PUBBL STAZ ZOOL NAPOLI, V28, P32; Mable BK, 1998, BIOESSAYS, V20, P453, DOI 10.1002/(SICI)1521-1878(199806)20:6<453::AID-BIES3>3.0.CO;2-N; Morrison SJ, 2006, NATURE, V441, P1068, DOI 10.1038/nature04956; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; PEDERSEN P.M., 1984, OPERA BOT, V74, P1; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Quatrano RS, 1997, COLD SPRING HARB SYM, V62, P65; Robinson KR, 1997, DEV BIOL, V187, P125, DOI 10.1006/dbio.1997.8600; RYCHLIK W, 1989, NUCLEIC ACIDS RES, V17, P8543, DOI 10.1093/nar/17.21.8543; Scheres B, 1999, ANNU REV PLANT PHYS, V50, P505, DOI 10.1146/annurev.arplant.50.1.505; Schneider SQ, 2003, ANNU REV GENET, V37, P221, DOI 10.1146/annurev.genet.37.110801.142443; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; STEBBINS GL, 1980, AM NAT, V115, P342, DOI 10.1086/283565; Tusher VG, 2001, P NATL ACAD SCI USA, V98, P5116, DOI 10.1073/pnas.091062498; Vandesompele J, 2002, GENOME BIOL, V3, DOI 10.1186/gb-2002-3-7-research0034; WILLSON MF, 1981, ANN MO BOT GARD, V68, P275, DOI 10.2307/2398799	34	68	68	0	10	COMPANY BIOLOGISTS LTD	CAMBRIDGE	BIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND	0950-1991	1477-9129		DEVELOPMENT	Development	APR 15	2008	135	8					1503	1512		10.1242/dev.016303			10	Developmental Biology	Developmental Biology	287LD	WOS:000254917700012	18339673	Bronze			2021-04-07	
J	Delaroque, N; Boland, W				Delaroque, Nicolas; Boland, Wilhelm			The genome of the brown alga Ectocarpus siliculosus contains a series of viral DNA pieces, suggesting an ancient association with large dsDNA viruses	BMC EVOLUTIONARY BIOLOGY			English	Article							ORIGIN; GENE; POLYDNAVIRUS; SEQUENCE; EVOLUTION; FAMILIES; INSIGHTS; ROLES; REPLICATION; COMMON	Background: Ectocarpus siliculosus virus-1 (EsV-1) is a lysogenic dsDNA virus belonging to the super family of nucleocytoplasmic large DNA viruses (NCLDV) that infect Ectocarpus siliculosus, a marine filamentous brown alga. Previous studies indicated that the viral genome is integrated into the host DNA. In order to find the integration sites of the viral genome, a genomic library from EsV-1-infected algae was screened using labelled EsV-1 DNA. Several fragments were isolated and some of them were sequenced and analyzed in detail. Results: Analysis revealed that the algal genome is split by a copy of viral sequences that have a high identity to EsV-1 DNA sequences. These fragments are interspersed with DNA repeats, pseudogenes and genes coding for products involved in DNA replication, integration and transposition. Some of these gene products are not encoded by EsV-1 but are present in the genome of other members of the NCLDV family. Further analysis suggests that the Ectocarpus algal genome contains traces of the integration of a large dsDNA viral genome; this genome could be the ancestor of the extant NCLDV genomes. Furthermore, several lines of evidence indicate that the EsV-1 genome might have originated in these viral DNA pieces, implying the existence of a complex integration and recombination system. A protein similar to a new class of tyrosine recombinases might be a key enzyme of this system. Conclusion: Our results support the hypothesis that some dsDNA viruses are monophyletic and evolved principally through genome reduction. Moreover, we hypothesize that phaeoviruses have probably developed an original replication system.	[Delaroque, Nicolas; Boland, Wilhelm] Max Planck Inst Chem Okol, D-07745 Jena, Germany	Delaroque, N (corresponding author), Max Planck Inst Chem Okol, Beutenberg Campus,Hans Knoll Str 8, D-07745 Jena, Germany.	ndelaroque@ice.mpg.de; boland@ice.mpg.de	Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			ACHULTZ J, 1998, P NATL ACAD SCI USA, V95, P5857; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Bamford DH, 2005, CURR OPIN STRUC BIOL, V15, P655, DOI 10.1016/j.sbi.2005.10.012; BELL PJ, 2006, J THEOR BIOL; Bell PJL, 2001, J MOL EVOL, V53, P251; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; CAMPO ED, 1997, PHYCOLOGIA, V36, P186; Claverie JM, 2006, GENOME BIOL, V7, DOI 10.1186/gb-2006-7-6-110; Delaroque N, 2003, J MOL EVOL, V57, P613, DOI 10.1007/s00239-003-2501-y; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Deneke J, 2004, J BIOL CHEM, V279, P53699, DOI 10.1074/jbc.M409001200; Deng LQ, 2000, VIROLOGY, V269, P440, DOI 10.1006/viro.2000.0248; Dunigan DD, 2006, VIRUS RES, V117, P119, DOI 10.1016/j.virusres.2006.01.024; Espagne E, 2004, SCIENCE, V306, P286, DOI 10.1126/science.1103066; Filee J, 2002, J MOL EVOL, V54, P763, DOI 10.1007/s00239-001-0078-x; Finn RD, 2006, NUCLEIC ACIDS RES, V34, pD247, DOI 10.1093/nar/gkj149; Forterre P, 2006, VIRUS RES, V117, P5, DOI 10.1016/j.virusres.2006.01.010; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Iyer LA, 2006, VIRUS RES, V117, P156, DOI 10.1016/j.virusres.2006.01.009; Iyer LM, 2005, NUCLEIC ACIDS RES, V33, P3875, DOI 10.1093/nar/gki702; Iyer LM, 2001, J VIROL, V75, P11720, DOI 10.1128/JVI.75.23.11720-11734.2001; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; Koonin EV, 2006, BIOL DIRECT, V1, DOI 10.1186/1745-6150-1-29; Kroemer JA, 2004, ANNU REV ENTOMOL, V49, P431, DOI 10.1146/annurev.ento.49.072103.120132; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; Lee AM, 1998, VIROLOGY, V248, P35, DOI 10.1006/viro.1998.9245; Lehman IR, 1999, J BIOL CHEM, V274, P28059, DOI 10.1074/jbc.274.40.28059; Litman GW, 2005, NAT REV IMMUNOL, V5, P866, DOI 10.1038/nri1712; Moreira D, 2005, SCIENCE, V308, DOI 10.1126/science.1110820; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Nunes-Duby SE, 1998, NUCLEIC ACIDS RES, V26, P391, DOI 10.1093/nar/26.2.391; O'Day DH, 2006, BIOCHEM BIOPH RES CO, V346, P879, DOI 10.1016/j.bbrc.2006.05.204; Raoult D, 2004, SCIENCE, V306, P1344, DOI 10.1126/science.1101485; Sambrook J., 1989, MOL CLONING LAB MANU; SPEYER JF, 1965, BIOCHEM BIOPH RES CO, V21, P6, DOI 10.1016/0006-291X(65)90417-1; Suhre K, 2005, P NATL ACAD SCI USA, V102, P14689, DOI 10.1073/pnas.0506465102; Suhre K, 2005, J VIROL, V79, P14095, DOI 10.1128/JVI.79.22.14095-14101.2005; Takemura M, 2001, J MOL EVOL, V52, P419, DOI 10.1007/s002390010171; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Villarreal LP, 2000, J VIROL, V74, P7079, DOI 10.1128/JVI.74.15.7079-7084.2000; Viswanathan M, 1998, GENETICS, V149, P7; Webb BA, 2006, VIROLOGY, V347, P160, DOI 10.1016/j.virol.2005.11.010; Wilson WH, 2005, SCIENCE, V309, P1090, DOI 10.1126/science.1113109	47	29	29	0	8	BMC	LONDON	CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	1471-2148			BMC EVOL BIOL	BMC Evol. Biol.	APR 12	2008	8								110	10.1186/1471-2148-8-110			12	Evolutionary Biology; Genetics & Heredity	Evolutionary Biology; Genetics & Heredity	298UW	WOS:000255713600001	18405387	DOAJ Gold, Green Published			2021-04-07	
J	Sekimoto, S; Beakes, GW; Gachon, CMM; Muller, DG; Kupper, FC; Honda, D				Sekimoto, Satoshi; Beakes, Gordon W.; Gachon, Claire M. M.; Mueller, Dieter G.; Kuepper, Frithjof C.; Honda, Daiske			The development, ultrastructural cytology, and molecular phylogeny of the basal oomycete Eurychasma dicksonii, infecting the filamentous phaeophyte algae Ectocarpus siliculosus and Pylaiella littoralis	PROTIST			English	Article						brown algae; infection; oomycete; phylogeny; sporogenesis; UGA codon reassignment	FINE-STRUCTURE; HALIPHTHOROS-MILFORDENSIS; PLASMODIOPHORA-BRASSICAE; MITOCHONDRIAL GENOME; ELECTRON-MICROSCOPY; SAPROLEGNIA-FERAX; GENETIC-CODE; MARINE; ZOOSPORES; PARASITE	The morphological development, ultrastructural cytology, and molecular phylogeny of Eurychasma dicksonii, a holocarpic oomycete endoparasite of phaeophyte algae, were investigated in laboratory cultures. Infection of the host algae by E. dicksonii is initiated by an adhesorium-like infection apparatus. First non-walled, the parasite cell developed a cell wall and numerous large vacuoles once it had almost completely filled the infected host cell (foamy stage). Large-scale cytoplasmic changes led to the differentiation of a sporangium with peripheral primary cysts. Secondary zoospores appeared to be liberated from the primary cysts in the internal space left after the peripheral spores differentiated. These zoospores contained two phases of peripheral vesicles, most likely homologous to the dorsal encystment vesicles and K-bodies observed in other oomycetes. Following zoospore liberation the walls of the empty cyst were left behind, forming the so-called net sporangium, a distinctive morphological feature of this genus. The morphological and ultrastructural features of Eurychasma were discussed in relation to similarities with other oomycetes. Both SSU rRNA and COII trees pointed to a basal position of Eurychasma among the Oomycetes. The cox2 sequences also revealed that the UGA codon encoded tryptophan, constituting the first report of stop codon reassignment in an oomycete mitochondrion. (c) 2007 Elsevier GmbH. All rights reserved.	[Honda, Daiske] Konan Univ, Dept Biol, Fac Sci & Engn, Kobe, Hyogo 6588501, Japan; [Sekimoto, Satoshi] Konan Univ, Grad Sch Nat Sci, Kobe, Hyogo 6588501, Japan; [Beakes, Gordon W.] Newcastle Univ, Div Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England; [Gachon, Claire M. M.; Kuepper, Frithjof C.] Dunstaffnage Marine Res Lab, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland; [Gachon, Claire M. M.] Univ Paris 11, CNRS, Inst Biotechnol Plantes, UMR 8618, F-91405 Orsay, France; [Mueller, Dieter G.] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany	Honda, D (corresponding author), Konan Univ, Dept Biol, Fac Sci & Engn, Kobe, Hyogo 6588501, Japan.	dhonda@konan-u.ac.jp	Gachon, Claire/C-2787-2009; GenePool, The/D-8812-2012	Honda, Daiske/0000-0002-3436-4561; Gachon, Claire/0000-0002-3702-7472; Kuepper, Frithjof/0000-0003-1273-7109	Medical Research CouncilUK Research & Innovation (UKRI)Medical Research Council UK (MRC)European Commission [MR/K001744/1] Funding Source: Medline; Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [NE/D521522/1] Funding Source: researchfish		AIST JR, 1971, CAN J BOTANY, V49, P2023, DOI 10.1139/b71-284; ALEEM AA, 1950, NATURE, V165, P119, DOI 10.1038/165119a0; Aleem AA, 1955, ARK BOT, V3, P1; ALEEM AA, 1950, MEDDELANDEN GOTEBORG, V18, P239; Anderson RA, 2005, ALGAL CULTURING TECH, P429, DOI DOI 10.1016/B978-012088426-1/50027-5; Beakes GW, 2006, PROCEEDINGS OF THE 8TH INTERNATIONAL MYCOLOGICAL CONGRESS, P7; Beakes G.W, 1994, GROWING FUNGUS, P339; BEAKES GW, 1989, SYST ASSOC SPEC VOL, V38, P325; BEAKES GW, 1978, T BRIT MYCOL SOC, V71, P25, DOI 10.1016/S0007-1536(78)80003-5; Beakes GW, 1998, FUNGAL GENET BIOL, V24, P45, DOI 10.1006/fgbi.1998.1072; BIMPONG CE, 1975, CAN J BOT, V53, P1310, DOI 10.1139/b75-158; BORTNICK RN, 1985, MYCOLOGIA, V77, P861, DOI 10.2307/3793298; BRACKER CE, 1973, P S HELD LONG ASHT R, P159; Chukanhom Kanit, 2003, Mycoscience, V44, P123, DOI 10.1007/s10267-003-0090-7; COFFEY MD, 1972, CAN J BOTANY, V50, P231, DOI 10.1139/b72-031; Cook KL, 2001, NOVA HEDWIGIA, V122, P231; Dearnaley JDW, 1996, MYCOL RES, V100, P39, DOI 10.1016/S0953-7562(96)80098-0; Gay JL, 1966, COLSTON PAP, P95; Glockling SL, 2000, CAN J BOT, V78, P1095, DOI 10.1139/cjb-78-8-1095; Grayburn WS, 2004, MYCOLOGIA, V96, P981, DOI 10.2307/3762082; GROSS P, 1993, EMBO J, V12, P1735, DOI 10.1002/j.1460-2075.1993.tb05821.x; Hakariya Masateru, 2007, Mycoscience, V48, P169, DOI 10.1007/s10267-007-0355-7; Hakariya Masateru, 2002, Mycoscience, V43, P119, DOI 10.1007/s102670200018; HEATH IB, 1971, Z ZELLFORSCH MIK ANA, V112, P371; HELD AA, 1981, BOT REV, V47, P451, DOI 10.1007/BF02860539; Hudspeth DSS, 2000, MYCOLOGIA, V92, P674, DOI 10.2307/3761425; JENNEBORG LH, 1977, BOT MAR, V20, P499, DOI 10.1515/botm.1977.20.8.499; JUKES TH, 1990, EXPERIENTIA, V46, P1117, DOI 10.1007/BF01936921; KARLOVSKY P, 1992, J MOL EVOL, V34, P254, DOI 10.1007/BF00162974; Katsaros C, 2006, ANN BOT-LONDON, V97, P679, DOI 10.1093/aob/mcl023; KONNO K, 1988, Bulletin of the National Science Museum Series B (Botany), V14, P119; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Leedale GF., 1967, EUGLENOID FLAGELLATE, V1st; LEHNEN LP, 1989, PROTOPLASMA, V149, P163, DOI 10.1007/BF01322988; Magnus P., 1905, HEDWIGIA, V44, P347; Martin FN, 2007, CURR GENET, V51, P285, DOI 10.1007/s00294-007-0121-6; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; Nakayama Takeshi, 1996, Phycological Research, V44, P47, DOI 10.1111/j.1440-1835.1996.tb00037.x; OLSON LW, 1984, NORD J BOT, V4, P681, DOI 10.1111/j.1756-1051.1984.tb01994.x; OVERTON SV, 1983, CAN J BOT, V61, P1165, DOI 10.1139/b83-123; PITERSEN HE, 2005, KGL DANSKE VIDENSKAB, V5, P439; PUESCHEL CM, 1985, CAN J BOT, V63, P409, DOI 10.1139/b85-049; Raghukumar C, 1980, CAN J BOT, V58, P2557, DOI [10.1139/b80-298, DOI 10.1139/B80-298]; RANDOLPH LR, 1992, MYCOLOGIA, V84, P768, DOI 10.2307/3760387; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; SCHNEPF E, 1978, ARCH MICROBIOL, V116, P133, DOI 10.1007/BF00406028; SEKIMOTO S, IN PRESS MYCOL RES; Sekimoto Satoshi, 2007, Mycoscience, V48, P212, DOI 10.1007/s10267-007-0357-5; SPARROW F. K., 1934, DANSK BOT ARKIV, V8, P1; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES; SPARROW FK, 1969, ARCH MIKROBIOL, V66, P129, DOI 10.1007/BF00410220; Swire J, 2005, J MOL EVOL, V60, P128, DOI 10.1007/s00239-004-0077-9; Takemoto D, 2003, PLANT J, V33, P775, DOI 10.1046/j.1365-313X.2003.01673.x; TEMMINK JHM, 1969, CAN J BOTANY, V47, P421, DOI 10.1139/b69-057; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; WILLIAMS PH, 1970, PHYTOPATHOLOGY, V60, P1557, DOI 10.1094/Phyto-60-1557; WRIGHT P, 1979, T R IRISH ACAD, V26, P369	58	37	43	1	16	ELSEVIER GMBH, URBAN & FISCHER VERLAG	JENA	OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY	1434-4610			PROTIST	Protist	APR	2008	159	2					299	318		10.1016/j.protis.2007.11.004			20	Microbiology	Microbiology	290KP	WOS:000255122000011	18243049				2021-04-07	
J	Meints, RH; Ivey, RG; Lee, AM; Choi, TJ				Meints, Russel H.; Ivey, Richard G.; Lee, Amy M.; Choi, Tae-Jin			Identification of two virus integration sites in the brown alga Feldmannia chromosome	JOURNAL OF VIROLOGY			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; GENOME; HOST; MIMIVIRUS; SEQUENCE; GENE	Two similar, large double-stranded DNA viruses, Feldmannia species virus 158 (FsV-158) and FsV-178, replicate only in the unilocular reproductive cells (sporangia) of a brown filamentous alga in the genus Feldmannia. Virus particles are not present in vegetative cells but they are produced in the sporangia formed on vegetative filaments that have been transferred newly into culture. Thus, we proposed that these viruses exist in the vegetative cells in a latent form (R. G. Ivey, E. C. Henry, A. M. Lee, L. Klepper, S. K. Krueger, and R. H. Meints, Virology 220:267-273, 1996). In this article we present evidence that the two FsV genomes are integrated into the host genome during vegetative growth. The FsV genome integration sites were identified by cloning the regions where the FsV genome is linked to the host DNA. FsV-158 and FsV-178 are integrated into two distinct locations in the algal genome. In contrast, the integration sites in the two viral genomes are identical. Notably, the integration sites in the host and viruses contain GC and CG dinucleotide sequences, respectively, from which the GC sequences are recovered at both host-virus junctions. The splice sites in the two FsV genomes are predicted to form a stem-loop structure with the CG dinucleotide in the loop portion.	[Choi, Tae-Jin] Pukyong Natl Univ, Dept Microbiol, Pusan 608737, South Korea; [Meints, Russel H.; Lee, Amy M.] Oregon State Univ, Ctr Gene Res & Biotechnol, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA; [Ivey, Richard G.] Univ Washington, Fred Hutchinson Canc Res Ctr, Seattle, WA 98195 USA	Choi, TJ (corresponding author), Pukyong Natl Univ, Dept Microbiol, 599-1 Daeyeon 3-Dong, Pusan 608737, South Korea.	choitj@pknu.ac.kr					ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Claverie JM, 2006, VIRUS RES, V117, P133, DOI 10.1016/j.virusres.2006.01.008; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; ENGELMAN A, 1991, CELL, V67, P1211, DOI 10.1016/0092-8674(91)90297-C; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; LANDY A, 1989, ANNU REV BIOCHEM, V58, P913, DOI 10.1146/annurev.biochem.58.1.913; Lee AM, 1998, J PHYCOL, V34, P608, DOI 10.1046/j.1529-8817.1998.340608.x; Lee AM, 1998, VIROLOGY, V248, P35, DOI 10.1006/viro.1998.9245; LEE AM, 1995, VIROLOGY, V21, P474; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Park Y, 2007, VIRUS GENES, V34, P177, DOI 10.1007/s11262-006-0059-7; Raoult D, 2004, SCIENCE, V306, P1344, DOI 10.1126/science.1101485; Sambrook J., 1989, MOL CLONING LAB MANU; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Villarreal LP, 2000, J VIROL, V74, P7079, DOI 10.1128/JVI.74.15.7079-7084.2000; Wu XL, 2005, J VIROL, V79, P5211, DOI 10.1128/JVI.79.8.5211-5214.2005	21	10	10	0	4	AMER SOC MICROBIOLOGY	WASHINGTON	1752 N ST NW, WASHINGTON, DC 20036-2904 USA	0022-538X			J VIROL	J. Virol.	FEB	2008	82	3					1407	1413		10.1128/JVI.01983-07			7	Virology	Virology	253JH	WOS:000252514000035	18032486	Bronze, Green Published			2021-04-07	
J	Muller, DG; Gachon, CMM; Kupper, FC				Mueller, Dieter G.; Gachon, Claire M. M.; Kuepper, Frithjof C.			Axenic clonal cultures of filamentous brown algae: initiation and maintenance	CAHIERS DE BIOLOGIE MARINE			English	Article						antibiotics; axenic; clonal; culture; ectocarpus; phaeophyceae	ECTOCARPUS-SILICULOSUS ECTOCARPALES; MACROCYSTIS-PYRIFERA; PHAEOPHYCEAE; ULVA; LAMINARIALES; ZOOSPORES; GENOMICS; BIOFILMS; GROWTH; CHILE	The increasing number of molecular and biotechnological studies involving brown algae has led to a growing demand for bacteria-free cultures. We describe here simple methods to establish axenic clonal cultures of filamentous marine brown algae. Thallus fragments are exposed on agar plates to commercially available paper disks loaded with defined concentrations of antibiotics. Regenerating fragments are isolated and used to initiate axenic algal cultures in liquid medium or on agar. We found the following agents to be tolerated by algae and active against marine bacteria: ciprofloxacin, chloramphenicol, rifampicin, polymyxin B, and kanamycin. Axenic agar cultures are maintained by serial transfer on agar, or re-introduced into liquid medium. Although we recommend transfer of agar cultures in 2-3 month intervals, we have observed survival times on agar of up to 2 years without attention.	[Gachon, Claire M. M.; Kuepper, Frithjof C.] Dunstaffnage Marine Res Lab, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland; [Mueller, Dieter G.] Fachbereich Biol Univ, D-78457 Constance, Germany; [Gachon, Claire M. M.] Univ Paris 11, Inst Plant Biotechnol, CNRS, UMR8618, F-91405 Orsay, France	Kupper, FC (corresponding author), Dunstaffnage Marine Res Lab, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland.	fck@sams.ac.uk	Gachon, Claire/C-2787-2009	Gachon, Claire/0000-0002-3702-7472			AGUIRRELIPPERHEIDE M, 1993, J PHYCOL, V29, P243, DOI 10.1111/j.0022-3646.1993.00243.x; BOALCH GT, 1961, J MAR BIOL ASSOC UK, V41, P287, DOI 10.1017/S0025315400023912; BOALCH GT, 1961, J MAR BIOL ASSOC UK, V41, P279, DOI 10.1017/S0025315400023900; BRADLEY PM, 1988, PLANT CELL TISS ORG, V12, P55, DOI 10.1007/BF00043107; Gachon CMM, 2007, GENE, V406, P51, DOI 10.1016/j.gene.2007.05.018; Green DH, 2004, FEMS MICROBIOL ECOL, V47, P345, DOI 10.1016/S0168-6496(03)00298-8; Hayden HS, 2003, EUR J PHYCOL, V38, P277, DOI 10.1080/1364253031000136321; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; LOISEAUX S, 1978, Revue Algologique, V13, P333; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; Marshall K, 2006, MICROB ECOL, V52, P302, DOI 10.1007/s00248-006-9060-x; Matsuo Y, 2005, SCIENCE, V307, P1598, DOI 10.1126/science.1105486; Matsuo Y, 2003, ENVIRON MICROBIOL, V5, P25, DOI 10.1046/j.1462-2920.2003.00382.x; Milner D. G., 2001, SPRINGER INDEX VIRUS, P732; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; Muller DG, 1996, PROTOPLASMA, V193, P58, DOI 10.1007/BF01276634; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Oudot-Le Secq MP, 2001, J MOL EVOL, V53, P80, DOI 10.1007/s002390010196; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Qian PY, 2007, MAR BIOTECHNOL, V9, P399, DOI 10.1007/s10126-007-9001-9; Starr R.C., 1987, Journal of Phycology, V23, P1; Tait K, 2005, ENVIRON MICROBIOL, V7, P229, DOI 10.1111/j.1462-2920.2004.00706.x; Weinberger F, 2007, J PHYCOL, V43, P235, DOI 10.1111/j.1529-8817.2007.00329.x; Westermeier R, 2006, AQUAC RES, V37, P164, DOI 10.1111/j.1365-2109.2005.01414.x; Westermeier R, 2007, J APPL PHYCOL, V19, P215, DOI 10.1007/s10811-006-9126-7; Wheeler GL, 2006, PLANT CELL ENVIRON, V29, P608, DOI 10.1111/j.1365-3040.2005.01440.x	29	22	23	3	17	CAHIERS DE BIOLOGIE MARINE	ROSCOFF	STATION BIOLOGIQUE PLACE GEORGES TEISSIER, 29680 ROSCOFF, FRANCE	0007-9723	2262-3094		CAH BIOL MAR	Cah. Biol. Mar.		2008	49	1					59	65					7	Marine & Freshwater Biology	Marine & Freshwater Biology	287FU	WOS:000254903200005					2021-04-07	
J	Billoud, B; Le Bail, A; Charrier, B				Billoud, Bernard; Le Bail, Aude; Charrier, Benedicte			A stochastic 1D nearest-neighbour automaton models early development of the brown alga Ectocarpus siliculosus	FUNCTIONAL PLANT BIOLOGY			English	Article; Proceedings Paper	5th International Workshop on Functional Structural Plant Models	NOV 04-09, 2007	Napier, NEW ZEALAND			cell communication; filament; morphogenesis; multicellularity; optimisation; Phaeophyceae	PATTERN-FORMATION; GROWTH; SIMULATION; PHAEOPHYCEAE; DIFFERENTIATION; PROLIFERATION; LANDSCAPES; CYTOKININS; MORPHOLOGY; GENETICS	Early development of the filamentous brown alga Ectocarpus siliculosus (Dillwyn) Lyngbye involves two cell types that are arranged in a polymorphic, but constrained, pattern. The present study aimed to decipher the cellular processes responsible for the establishment of this pattern. Thorough observations characterised five different events of division and differentiation that occurred during the early development. The hypothesis that a local control is responsible for these processes was tested. Todo so, Ectomat, a stochastic automaton in which each cell only interacts with its closest neighbour(s), was created. The probabilities for the five events were adjusted to fit to the observations. Simulations with Ectomat reconstructed most of the essential properties of the sporophyte development, in terms of cell-type proportion, relative position and growth dynamics. The whole organism properties emerged by applying local transition rules. In conclusion, no global position information system was required at this development stage. Randomly occurring cell events, driven by simple contact interactions, are sufficient to account for the early filament development and establishment of the cell-type pattern of E. siliculosus.	[Billoud, Bernard] Univ Paris 06, F-75005 Paris, France; [Le Bail, Aude; Charrier, Benedicte] Univ Paris 06, Vegetaux Marins & Biomol UMR7139, Biol Stn, F-29682 Roscoff, France; [Le Bail, Aude; Charrier, Benedicte] CNRS, Biol Stn, Vegetaux Marins & Biomol UMR7139, F-29682 Roscoff, France	Billoud, B (corresponding author), Univ Paris 06, MB1202, F-75005 Paris, France.	bernard.billoud@snv.jussieu.fr		Charrier, Benedicte/0000-0001-5721-1640; Billoud, Bernard/0000-0002-5140-8087			Akberdin IR, 2007, J BIOINF COMPUT BIOL, V5, P641, DOI 10.1142/S0219720007002862; Alber M., 2002, IMA, V134, P12; Baker CTH, 1998, J MATH BIOL, V37, P341, DOI 10.1007/s002850050133; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Basu S, 2002, PLANT PHYSIOL, V130, P292, DOI 10.1104/pp.004747; BRADLEY PM, 1991, J PHYCOL, V27, P317, DOI 10.1111/j.0022-3646.1991.00317.x; Charrier B, 2008, NEW PHYTOL, V177, P319, DOI 10.1111/j.1469-8137.2007.02304.x; Cho GY, 2004, J PHYCOL, V40, P921, DOI 10.1111/j.1529-8817.2004.03160.x; Coelho SM, 2007, GENE, V406, P152, DOI 10.1016/j.gene.2007.07.025; Coen E, 2004, P NATL ACAD SCI USA, V101, P4728, DOI 10.1073/pnas.0306308101; CORBIT JD, 1993, COMPUT GRAPH, V17, P85, DOI 10.1016/0097-8493(93)90055-E; Cove DJ, 2000, J EXP BOT, V51, P831, DOI 10.1093/jexbot/51.346.831; Davis RH, 2004, NAT REV GENET, V5, p69A, DOI 10.1038/nrg1250; DEKOSTER CG, 1987, ACTA BIOTHEOR, V36, P249, DOI 10.1007/BF02329786; DRAISMA SGA, 2003, COLLECTED REV CELEBR, P87; EVANS LV, 1991, J PHYCOL, V27, P322, DOI 10.1111/j.0022-3646.1991.00322.x; Federl P, 2004, LECT NOTES COMPUT SC, V3037, P65; GARBARY DJ, 1992, PROGR PHYCOLOGICAL R, V8, P143; Garcia-Bellido AC, 1998, INT J DEV BIOL, V42, P353; Hammel M, 1996, PROC GRAPH INTERF, P246; Heinlein M, 2004, INT REV CYTOL, V235, P93, DOI 10.1016/S0074-7696(04)35003-5; HOEL DG, 1971, BIOMETRICS, V27, P191, DOI 10.2307/2528937; Holloway DM, 2002, ANN BOT-LONDON, V89, P409, DOI 10.1093/aob/mcf061; HONDA H, 1973, J THEOR BIOL, V42, P461, DOI 10.1016/0022-5193(73)90241-5; Izaguirre JA, 2004, BIOINFORMATICS, V20, P1129, DOI 10.1093/bioinformatics/bth050; Kawai H, 2007, J PHYCOL, V43, P186, DOI 10.1111/j.1529-8817.2006.00308.x; KIRKPATRICK S, 1983, SCIENCE, V220, P671, DOI 10.1126/science.220.4598.671; LAINDENM.A, 1971, J THEOR BIOL, V30, P455; LEBAIL A, 2008, J PHYCOLOGY IN PRESS; LINDENMAYER A, 1975, J THEOR BIOL, V54, P3, DOI 10.1016/S0022-5193(75)80051-8; Luck J, 1999, ACTA BIOTHEOR, V47, P329, DOI 10.1023/A:1002659209511; Mariscal V, 2007, MOL MICROBIOL, V65, P1139, DOI 10.1111/j.1365-2958.2007.05856.x; Meeks JC, 2002, MICROBIOL MOL BIOL R, V66, P94, DOI 10.1128/MMBR.66.1.94-121.2002; METROPOLIS N, 1953, J CHEM PHYS, V21, P1087, DOI 10.1063/1.1699114; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; PEDERSEN M, 1973, PHYSIOL PLANTARUM, V28, P101, DOI 10.1111/j.1399-3054.1973.tb01158.x; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Prusinkiewicz P, 2006, CURR OPIN PLANT BIOL, V9, P83, DOI 10.1016/j.pbi.2005.11.015; Prusinkiewicz P., 1990, ALGORITHMIC BEAUTY P; R Development Core Team, 2007, R LANG ENV STAT COMP; REMPHREY WR, 1983, CAN J BOT, V61, P2430, DOI 10.1139/b83-267; RICHARDSON D, 1973, P CAMB PHILOS SOC, V74, P563; Rolland-Lagan AG, 2005, J THEOR BIOL, V232, P157, DOI 10.1016/j.jtbi.2004.04.045; Silva HS, 2003, PHYSICA A, V322, P555, DOI 10.1016/S0378-4371(02)01807-1; Stadler PF, 1996, J MATH CHEM, V20, P1, DOI 10.1007/BF01165154; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Stern CD, 2006, CURR OPIN GENET DEV, V16, P413, DOI 10.1016/j.gde.2006.06.005; Stirk WA, 2003, PLANT GROWTH REGUL, V41, P13, DOI 10.1023/A:1027376507197; WEINBERGER E, 1990, BIOL CYBERN, V63, P325, DOI 10.1007/BF00202749; Yoon HS, 1998, SCIENCE, V282, P935, DOI 10.1126/science.282.5390.935	50	11	11	1	6	CSIRO PUBLISHING	CLAYTON	UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA	1445-4408	1445-4416		FUNCT PLANT BIOL	Funct. Plant Biol.		2008	35	9-10					1014	1024		10.1071/FP08036			11	Plant Sciences	Plant Sciences	370WO	WOS:000260794000024	32688850				2021-04-07	
J	Gachon, CMM; Day, JG; Campbell, CN; Proschold, T; Saxon, RJ; Kupper, FC				Gachon, Claire M. M.; Day, John G.; Campbell, Christine N.; Proschold, Thomas; Saxon, Rachel J.; Kupper, Frithjof C.			The Culture Collection of Algae and Protozoa (CCAP): A biological resource for protistan genomics	GENE			English	Article						biodiversity; biological resource centre; cyanobacterium; integrated database; model organism; natural variation	COMPLETE NUCLEOTIDE-SEQUENCE; MITOCHONDRIAL-DNA SEQUENCE; CHLOROPLAST GENOME; GREEN-ALGA; GENE CONTENT; CYANIDIOSCHYZON-MEROLAE; PLASTID GENOME; LAND PLANTS; DICTYOSTELIUM-DISCOIDEUM; TETRAHYMENA-THERMOPHILA	CCAP, the largest European protistan culture collection, is based at the Scottish Association for Marine Science near Oban, Scotland (http:// www.ccap.ac.uk). The Collection comprises more than 2700 strains in the public domain, of which 1050 are marine algae, 1300 freshwater algae, and 350 protozoa. The primary mission of CCAP is to maintain and distribute defined cultures and their associated information to its customers. It also has a support and advisory function on all aspects of protistan science. In addition, it is involved in the training of students and researchers in algal identification and culture techniques. In light of the increasing number of fully sequenced protists, the CCAP is striving to provide targeted services and support to workers involved in all aspects of genomic research. At present, the Collection holds several hundred strains of genomic model taxa including: Acanthamoeba, Cafeteria, Cercomonas, Chlamydomonas, Chlorella, Cyanophora, Dictyostelium, Dunaliella, Ectocarpus, Emiliania, Euglena, Micromonas, Naegleria, Nephroselmis, Paramecium, Pavlova, Phaeodactylum, Porphyra, Pseudendoclonium, Pylaiella, Rhodomonas, Scenedesmus, Staurastrum, Tetrahymena, Thalassiosira, Volvox and Zygnema. These strains provide a defined representation of natural variation within model organisms, an increasingly useful resource for post-genomics approaches. Our aim over the next 2-5 years is to add value to the Collection by increasing the number of genome model species, and by offering an integrated, up-to-date, easy-to-use resource that would provide curated information on our strain holdings. In collaboration with other major Biological Resource Centres worldwide, we intend to build a hub providing access to both protistan cultures and their associated bioinformatics data. (C) 2007 Elsevier B.V. All rights reserved.	[Gachon, Claire M. M.; Day, John G.; Campbell, Christine N.; Proschold, Thomas; Saxon, Rachel J.; Kupper, Frithjof C.] Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland	Gachon, CMM (corresponding author), Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland.	claire.gachon@sams.ac.uk	Day, John/N-5913-2014; Gachon, Claire/C-2787-2009	Proeschold, Thomas/0000-0002-7858-0434; Kuepper, Frithjof/0000-0003-1273-7109; Gachon, Claire/0000-0002-3702-7472	Natural Environment Research CouncilUK Research & Innovation (UKRI)NERC Natural Environment Research Council [dml010007] Funding Source: researchfish		Armbrust EV, 2004, SCIENCE, V306, P79, DOI 10.1126/science.1101156; Aury JM, 2006, NATURE, V444, P171, DOI 10.1038/nature05230; Barbier G, 2005, PLANT PHYSIOL, V137, P460, DOI 10.1104/pp.104.051169; Belanger AS, 2006, MOL GENET GENOMICS, V276, P464, DOI 10.1007/s00438-006-0156-2; Brunk CF, 2003, NUCLEIC ACIDS RES, V31, P1673, DOI 10.1093/nar/gkg270; Burger G, 1999, PLANT CELL, V11, P1675, DOI 10.1105/tpc.11.9.1675; BURGER G, 1995, J MOL BIOL, V245, P522, DOI 10.1006/jmbi.1994.0043; Cavalcanti ARO, 2004, CHROMOSOMA, V113, P69, DOI 10.1007/s00412-004-0295-3; Chesnick JM, 2000, NUCLEIC ACIDS RES, V28, P2512, DOI 10.1093/nar/28.13.2512; Day JG, 2004, NOVA HEDWIGIA, V79, P27, DOI 10.1127/0029-5035/2004/0079-0027; DAY JG, 2004, PHYCOLOGIST, V67, P6; de Cambiaire JC, 2006, BMC EVOL BIOL, V6, DOI 10.1186/1471-2148-6-37; DEKONING AP, 2006, BMC EVOL BIOL, P4; Denovan-Wright  EM, 1998, PLANT MOL BIOL, V36, P285, DOI 10.1023/A:1005995718091; Derelle E, 2006, P NATL ACAD SCI USA, V103, P11647, DOI 10.1073/pnas.0604795103; Douglas S, 2001, NATURE, V410, P1091, DOI 10.1038/35074092; Douglas SE, 1999, J MOL EVOL, V48, P236, DOI 10.1007/PL00006462; Eichinger L, 2005, NATURE, V435, P43, DOI 10.1038/nature03481; Eisen JA, 2006, PLOS BIOL, V4, P1620, DOI 10.1371/journal.pbio.0040286; Gilad Y, 2006, CURR OPIN GENET DEV, V16, P553, DOI 10.1016/j.gde.2006.09.005; Gilson PR, 2006, P NATL ACAD SCI USA, V103, P9566, DOI 10.1073/pnas.0600707103; Glockner G, 2000, J MOL EVOL, V51, P382; Gray MW, 1998, NUCLEIC ACIDS RES, V26, P865, DOI 10.1093/nar/26.4.865; Grossman AR, 2003, EUKARYOT CELL, V2, P1137, DOI 10.1128/EC.2.6.1137-1150.2003; Haag-Liautard C, 2007, NATURE, V445, P82, DOI 10.1038/nature05388; Hagopian JC, 2004, J MOL EVOL, V59, P464, DOI 10.1007/s00239-004-2638-3; HALLICK RB, 1993, NUCLEIC ACIDS RES, V21, P3537, DOI 10.1093/nar/21.15.3537; Hauth AM, 2005, NUCLEIC ACIDS RES, V33, P4433, DOI 10.1093/nar/gki757; Kowallik KV, 1995, PLANT MOL BIOL REP, V13, P336, DOI 10.1007/BF02669188; Kroymann J, 1998, J MOL EVOL, V47, P431, DOI 10.1007/PL00006400; Kuck U, 2000, GENE, V253, P13, DOI 10.1016/S0378-1119(00)00228-6; Lang BF, 1999, J EUKARYOT MICROBIOL, V46, P320, DOI 10.1111/j.1550-7408.1999.tb04611.x; Lang BF, 1997, NATURE, V387, P493, DOI 10.1038/387493a0; LEBLANC C, 1995, J MOL BIOL, V250, P484, DOI 10.1006/jmbi.1995.0392; Lemieux C, 2000, NATURE, V403, P649, DOI 10.1038/35001059; Matsuzaki M, 2004, NATURE, V428, P653, DOI 10.1038/nature02398; Maul JE, 2002, PLANT CELL, V14, P2659, DOI 10.1105/tpc.006155; Mitchell-Olds T, 2006, NATURE, V441, P947, DOI 10.1038/nature04878; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; Ogawa S, 2000, MOL GEN GENET, V263, P514, DOI 10.1007/s004380051196; Ohta N, 2003, DNA RES, V10, P67, DOI 10.1093/dnares/10.2.67; Ohta N, 1998, NUCLEIC ACIDS RES, V26, P5190, DOI 10.1093/nar/26.22.5190; Oudot-le Secq MP, 2002, EUR J PHYCOL, V37, P163, DOI 10.1017/S0967026202003542; Oudot-Le Secq MP, 2001, J MOL EVOL, V53, P80, DOI 10.1007/s002390010196; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; Pombert JF, 2006, CURR GENET, V50, P137, DOI 10.1007/s00294-006-0076-z; Pombert JF, 2005, MOL BIOL EVOL, V22, P1903, DOI 10.1093/molbev/msi182; Pombert JF, 2004, MOL BIOL EVOL, V21, P922, DOI 10.1093/molbev/msh099; POMBERT JF, 2006, BMC EVOL BIOL, P4; PRITCHARD AE, 1990, NUCLEIC ACIDS RES, V18, P173, DOI 10.1093/nar/18.1.173; Puerta MVS, 2005, DNA RES, V12, P151, DOI 10.1093/dnares/12.2.151; Puerta MVS, 2004, DNA RES, V11, P1, DOI 10.1093/dnares/11.1.1; Reith M, 1995, PLANT MOL BIOL REP, V13, P333, DOI 10.1007/BF02669187; Robbens S, 2007, MOL BIOL EVOL, V24, P956, DOI 10.1093/molbev/msm012; Rogers MB, 2007, MOL BIOL EVOL, V24, P54, DOI 10.1093/molbev/msl129; Secq MPO, 2006, CURR GENET, V49, P47, DOI 10.1007/s00294-005-0031-4; SIEMEISTER G, 1989, CURR GENET, V15, P435, DOI 10.1007/BF00376801; Slapeta J, 2006, MOL BIOL EVOL, V23, P23, DOI 10.1093/molbev/msj001; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Stirewalt VL, 1995, PLANT MOL BIOL REP, V13, P327, DOI 10.1007/BF02669186; Turmel M, 1999, PLANT CELL, V11, P1717, DOI 10.1105/tpc.11.9.1717; Turmel M, 2003, PLANT CELL, V15, P1888, DOI 10.1105/tpc.013169; Turmel M, 1999, P NATL ACAD SCI USA, V96, P10248, DOI 10.1073/pnas.96.18.10248; Turmel M, 2002, P NATL ACAD SCI USA, V99, P11275, DOI 10.1073/pnas.162203299; Turmel M, 2002, MOL BIOL EVOL, V19, P24, DOI 10.1093/oxfordjournals.molbev.a003979; TURMEL M, 2005, BMC EVOL BIOL, P3; Turmel M, 2006, MOL BIOL EVOL, V23, P1324, DOI 10.1093/molbev/msk018; Wakasugi T, 1997, P NATL ACAD SCI USA, V94, P5967, DOI 10.1073/pnas.94.11.5967; Ward N, 2001, NATURE, V414, P148, DOI 10.1038/35102737; WILLIAMSON P, 2007, MICROBIOL TODAY, V34, P46; WOLFF G, 1994, J MOL BIOL, V237, P75, DOI 10.1006/jmbi.1994.1210; 1994, REV UK MICROBIAL CUL	73	16	18	0	18	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	0378-1119	1879-0038		GENE	Gene	DEC 30	2007	406	1-2					51	57		10.1016/j.gene.2007.05.018			7	Genetics & Heredity	Genetics & Heredity	253EC	WOS:000252500500008	17614217				2021-04-07	
J	Kraufvelin, P				Kraufvelin, Patrik			Responses to nutrient enrichment, wave action and disturbance in rocky shore communities	AQUATIC BOTANY			English	Article						mesocosm; nutrient enrichment; canopy gap formation; rocky intertidal; algal colonisation; marine biodiversity	LONG-TERM CHANGES; SPECIES-DIVERSITY; MACROALGAL COMMUNITIES; NITROGEN REQUIREMENTS; FILAMENTOUS ALGAE; WATER-QUALITY; EUTROPHICATION; GROWTH; FOOD; ASSEMBLAGES	A high degree of resistance against nutrient enrichment has previously been demonstrated for macroalgal-dominated rocky shore communities in the presence of moderate to large amounts of macroinvertebrate grazers. To experimentally examine, under controlled conditions, the possible roles for this resistance of two other factors, i.e. disturbance (presence/absence of the macroalgal canopy itself) and wave action, the canopy algae and associated algal and animal assemblages were removed by scraping from approximately one third of the area of eight littoral mesocosms, subjected to two different wave action regimes. After this, excessive nutrients were added to four mesocosms with the factor nutrients fully crossed with the factor wave action with two replicate mesocosm basins of each nutrient/wave treatment combination. Disturbance was added to the design as a within-basin factor thus making up a split-plot experiment. The abundance of grazers was allowed to vary freely and under the influence of the treatments. After I I summer weeks, there were significant differences in community structure between nutrient enrichment levels for both algal and animal assemblages when examined by multivariate statistical techniques. Univariate analyses confirmed a significantly stimulated colonisation by green algae, mainly Ulva lactuca, in both disturbed (scraped) and undisturbed areas of nutrient-enriched mesocosms. In un-enriched mesocosms, the green algae were absent from undisturbed areas and rare in disturbed areas, where mainly brown Ectocarpus spp. and red algae had settled. Among the macrofauna, the total abundance of grazers was stimulated in nutrient-enriched mesocosms with individuals of the amphipod genus Gammarus and the isopod genus Jaera being especially numerous. With regard to wave action, no significant differences occurred in community structure, although there were indications of significant nutrient x wave effects for both the amount of exported red algae and the amount of accumulated brown algae. The study shows that eutrophication-related community shifts on rocky shores may occur very rapidly, regardless of the level of wave-energetic stress and the abundance of grazers, if the nutrient concentrations are high and the colonisation and growth of opportunistic algae are facilitated by disturbance such as (naturally or anthropogenically driven) canopy gap forming processes. (C) 2007 Elsevier B.V. All fights reserved.	Abo Akad Univ, FIN-20500 Turku, Finland	Kraufvelin, P (corresponding author), Abo Akad Univ, Akad Gatan 1, FIN-20500 Turku, Finland.	patrik.kraufvelin@abo.fi		Kraufvelin, Patrik/0000-0003-3224-8388			Back S, 2000, ANN BOT FENN, V37, P155; Barron C, 2003, ECOSYSTEMS, V6, P144, DOI 10.1007/s10021-002-0402-3; Benedetti-Cecchi L, 2001, MAR ECOL PROG SER, V214, P137, DOI 10.3354/meps214137; Berger R, 2003, AQUAT ECOL, V37, P1, DOI 10.1023/A:1022136900630; BOKN T, 1978, NORW J BOT, V25, P9; Bokn TL, 2003, ECOSYSTEMS, V6, P577, DOI 10.1007/s10021-002-0108-6; Bokn TL, 2002, HYDROBIOLOGIA, V484, P167, DOI 10.1023/A:1021365307438; Christie H, 2004, SCI MAR, V68, P189, DOI 10.3989/scimar.2004.68s1189; CLARKE KR, 1993, AUST J ECOL, V18, P117, DOI 10.1111/j.1442-9993.1993.tb00438.x; Cloern JE, 2001, MAR ECOL PROG SER, V210, P223, DOI 10.3354/meps210223; Connell J.H., 1985, P57; CONNELL JH, 1978, SCIENCE, V199, P1302, DOI 10.1126/science.199.4335.1302; Czerniawska-Kusza I, 2004, POL J ENVIRON STUD, V13, P579; DAYTON PK, 1971, ECOL MONOGR, V41, P351, DOI 10.2307/1948498; DEPAUW N, 1986, HYDROBIOLOGIA, V133, P237, DOI 10.1007/BF00005595; Diaz P, 2002, BOT MAR, V45, P267, DOI 10.1515/BOT.2002.026; Dick JTA, 2005, MAR ECOL PROG SER, V291, P151, DOI 10.3354/meps291151; DUARTE CM, 1995, OPHELIA, V41, P87, DOI 10.1080/00785236.1995.10422039; DUFFY JE, 1990, OECOLOGIA, V83, P267, DOI 10.1007/BF00317764; Edgar GJ, 2004, J EXP MAR BIOL ECOL, V312, P67, DOI 10.1016/j.jembe.2004.06.005; Eriksson BK, 2006, ECOLOGY, V87, P246, DOI 10.1890/05-0090; GEERTZHANSEN O, 1993, AQUAT BOT, V46, P101, DOI 10.1016/0304-3770(93)90039-Y; HAWKINS SJ, 1992, SYST ASSOC SPEC VOL, V46, P1; HAWKINS SJ, 1983, OCEANOGR MAR BIOL, V21, P195; Hayden HS, 2003, EUR J PHYCOL, V38, P277, DOI 10.1080/1364253031000136321; Hillebrand H, 2000, MAR ECOL PROG SER, V204, P27, DOI 10.3354/meps204027; HOCHBERG Y, 1988, BIOMETRIKA, V75, P800, DOI 10.1093/biomet/75.4.800; Jara VC, 2006, MAR ECOL PROG SER, V308, P37, DOI 10.3354/meps308037; Jenkins SR, 2005, MAR ECOL PROG SER, V287, P77, DOI 10.3354/meps287077; Karez R, 2004, AQUAT BOT, V78, P103, DOI 10.1016/j.aquabot.2003.09.008; Kiirikki M, 1996, EUR J PHYCOL, V31, P61, DOI 10.1080/09670269600651201; Kiirikki M, 1996, BOT MAR, V39, P133, DOI 10.1515/botm.1996.39.1-6.133; Kraufvelin P, 2002, HYDROBIOLOGIA, V484, P149, DOI 10.1023/A:1021313323367; Kraufvelin P, 2006, AQUAT BOT, V84, P199, DOI 10.1016/j.aquabot.2005.08.008; Kraufvelin P, 2004, ESTUAR COAST SHELF S, V61, P369, DOI 10.1016/j.ecss.2004.06.006; Kraufvelin P, 2007, ESTUAR COAST SHELF S, V72, P665, DOI 10.1016/j.ecss.2006.11.029; Kraufvelin P, 2006, ECOSYSTEMS, V9, P1076, DOI 10.1007/s10021-005-0188-1; LEWIS JR, 2004, ECOLOGY ROCKY SHORES; Little C, 1996, BIOL ROCKY SHORES; LITTLER MM, 1980, AM NAT, V116, P25, DOI 10.1086/283610; Lotze HK, 2000, OIKOS, V89, P46, DOI 10.1034/j.1600-0706.2000.890106.x; LOTZE HK, 2000, MAR ECOL PROG SER, V14, P1428; LUBCHENCO J, 1978, AM NAT, V112, P23, DOI 10.1086/283250; Mackey RL, 2001, ECOLOGY, V82, P3479, DOI 10.1890/0012-9658(2001)082[3479:TDDRII]2.0.CO;2; Mann K. H., 1982, ECOLOGY COASTAL WATE; MANN KH, 1973, SCIENCE, V182, P975, DOI 10.1126/science.182.4116.975; MENGE BA, 1987, AM NAT, V130, P730, DOI 10.1086/284741; Middelboe AL, 2000, PHYCOLOGIA, V39, P245, DOI 10.2216/i0031-8884-39-3-245.1; Moran MD, 2003, OIKOS, V100, P403, DOI 10.1034/j.1600-0706.2003.12010.x; Nielsen KJ, 2001, ECOL MONOGR, V71, P187, DOI 10.1890/0012-9615(2001)071[0187:BUATDF]2.0.CO;2; PAASCHE E, 1982, ESTUAR COAST SHELF S, V14, P237, DOI 10.1016/S0302-3524(82)80014-5; PAINE RT, 1981, ECOL MONOGR, V51, P145, DOI 10.2307/2937261; Patricio J, 2006, ECOL INDIC, V6, P43, DOI 10.1016/j.ecolind.2005.08.016; Pedersen MF, 1996, MAR ECOL PROG SER, V142, P261, DOI 10.3354/meps142261; Pedersen MF, 1997, MAR ECOL PROG SER, V161, P155, DOI 10.3354/meps161155; Pihl L, 1999, J SEA RES, V41, P281, DOI 10.1016/S1385-1101(99)00004-0; Raberg S, 2005, MAR ECOL PROG SER, V289, P131, DOI 10.3354/meps289131; RONNBERG O, 1992, AQUAT BOT, V42, P109, DOI 10.1016/0304-3770(92)90002-Z; SANTELICES B, 1984, MAR ECOL PROG SER, V19, P73, DOI 10.3354/meps019073; Schramm W, 1999, J APPL PHYCOL, V11, P69, DOI 10.1023/A:1008076026792; Sokal R.R., 1995, BIOMETRY, V3; SOUSA WP, 1979, ECOL MONOGR, V49, P227, DOI 10.2307/1942484; Thompson RC, 2002, ENVIRON CONSERV, V29, P168, DOI 10.1017/S0376892902000115; Underwood AJ., 1997, EXPT ECOLOGY THEIR L; Valdivia N, 2005, MAR ECOL PROG SER, V299, P45, DOI 10.3354/meps299045; Valdivia N, 2006, J SEA RES, V56, P271, DOI 10.1016/j.seares.2006.06.003; Valiela I, 1997, LIMNOL OCEANOGR, V42, P1105, DOI 10.4319/lo.1997.42.5_part_2.1105; Weaver MJ, 1997, CAN J FISH AQUAT SCI, V54, P2277, DOI 10.1139/cjfas-54-10-2277; Winer B.J., 1991, STAT PRINCIPLES EXPT, V(3rd ed; Worm B, 2002, NATURE, V417, P848, DOI 10.1038/nature00830; Worm B, 2000, LIMNOL OCEANOGR, V45, P339, DOI 10.4319/lo.2000.45.2.0339; Worm B, 2006, LIMNOL OCEANOGR, V51, P569, DOI 10.4319/lo.2006.51.1_part_2.0569	72	43	43	2	59	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	0304-3770	1879-1522		AQUAT BOT	Aquat. Bot.	NOV	2007	87	4					262	274		10.1016/j.aquabot.2007.06.011			13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	227OO	WOS:000250667700002					2021-04-07	
J	Kupper, H; Seibert, S; Parameswaran, A				Kupper, Hendrik; Seibert, Sven; Parameswaran, Aravind			Fast, sensitive, and inexpensive alternative to analytical pigment HPLC: Quantification of chlorophylls and carotenoids in crude extracts by fitting with gauss peak spectra	ANALYTICAL CHEMISTRY			English	Article							METAL-SUBSTITUTED CHLOROPHYLLS; IN-VIVO; INDUCED INHIBITION; PHOTOSYNTHESIS	Quantification of pigments in complex. mixtures is an important task in the physiology of photosynthetic organisms, because pigment composition differs depending on the species, tissue, and physiological state. Currently available methods, however, are either limited to very few pigments (classical UV/vis spectroscopic methods), or they are time-consuming, labor intensive, or costly (e.g., HPLC). Here we describe a UV/vis spectrophotometric method that is capable of a rapid (similar to 1 min/sample) and inexpensive (< 1 euro/sample) quantification of more than a dozen pigments in a crude extract, which means it is suitable for high-throughput screening applications. A detection limit of < 1 pmol for each pigment allows for determining the pigment composition in only 0.5 mu g of lyophilized leaves or algae. The method is based on the description of each pigment spectrum by a series of Gaussian peaks. A sample spectrum is then fitted by a linear combination of these "Gauss peak spectra" including an automatic correction of wavelength inaccuracy, baseline instability, sample turbidity, and effects of temperature/water content. Here we present the Gauss peak spectra from 350 to 750 nm for acetone solutions of all chlorophyll and carotenoid derivatives that are abundant (including conditions of Cd, Cu, or Zn stress) in leaves of higher plants, Euglena, brown algae, and various cyanobacteria like Anabaena and Trichodesmium: [Mg]-Ch1 a, b, c(1), c(2); pheophytin a, b; [Cd]-Ch1 a, b; [Cu]-Ch1 a, b; [Zn]-Ch1 a, b; antheraxanthin, aurochrome, beta-carotene, beta-cryptoxanthin, cis- and trans-canthaxanthin, diadinochrome (=diadinoxanthin 5,6-epoxide), cis- and trans-diadinoxanthin, diatoxanthin, cis- and trans-echinenone, fucoxanthin, lutein, myxoxanthophyll, neoxanthin, violaxanthin, and all three stereoisomers of zeaxanthin in acetone. We present extensive tests of our new quantification method for determining optimal and limiting conditions of its performance and for comparison with previous methods. Finally, we show application examples for Thlaspi fendleri (Chlorophyta), Euglena gracilisc (Euglenophyta), Ectocarpus siliculosus (Phaeophyta), and Trichodesmium erythraeum IMS101 (eyanobacteria).	Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt, D-78457 Constance, Germany; Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic; Univ S Bohemia, Inst Biol Phys, CZ-37005 Ceske Budejovice, Czech Republic	Kupper, H (corresponding author), Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt, D-78457 Constance, Germany.	hendrik.kuepper@uni-konstanz.de	Kupper, Hendrik/J-5152-2012	Kupper, Hendrik/0000-0003-0712-7023			ARNON DI, 1949, PLANT PHYSIOL, V24, P1, DOI 10.1104/pp.24.1.1; Britton G., 1995, CAROTENOIDS, V1B, P13; Colombo-Pallotta MF, 2006, J PHYCOL, V42, P1225, DOI 10.1111/j.1529-8817.2006.00287.x; DAVIES BH, 1988, HDB CHROMATOGRAPHY, P3; DOEGE M, 1999, THESIS; ENGLERT G, 1991, HELV CHIM ACTA, V74, P969, DOI 10.1002/hlca.19910740507; JOHANSEN JE, 1974, PHYTOCHEMISTRY, V13, P2261, DOI 10.1016/0031-9422(74)85038-7; JONES ID, 1977, J AGR FOOD CHEM, V25, P146, DOI 10.1021/jf60209a025; Kuepper Hendrik, 2006, V25, P67; Kupper H, 2007, NEW PHYTOL, V175, P655, DOI 10.1111/j.1469-8137.2007.02139.x; Kupper H, 2000, ANAL BIOCHEM, V286, P247, DOI 10.1006/abio.2000.4794; Kupper H, 1996, J EXP BOT, V47, P259, DOI 10.1093/jxb/47.2.259; Kupper H, 2004, PLANT PHYSIOL, V135, P2120, DOI 10.1104/pp.104.045963; Kupper H, 2003, FUNCT PLANT BIOL, V30, P1187, DOI 10.1071/FP03129; Kupper H, 2002, J PHYCOL, V38, P429, DOI 10.1046/j.1529-8817.2002.01148.x; Kupper H, 1998, PHOTOSYNTH RES, V58, P123, DOI 10.1023/A:1006132608181; LICHTMAN GS, 1983, GEOLOGY, V11, P592, DOI 10.1130/0091-7613(1983)11<592:TASCOT>2.0.CO;2; Naqvi KR, 1997, SPECTROCHIM ACTA A, V53, P2229; Naqvi KR, 2004, SPECTROCHIM ACTA A, V60, P2783, DOI 10.1016/j.saa.2004.01.017; NELIS HJCF, 1988, J LIPID RES, V29, P491; Porra Robert J., 2006, V25, P95; Ritchie RJ, 2006, PHOTOSYNTH RES, V89, P27, DOI 10.1007/s11120-006-9065-9; Rocchetta I, 2006, B ENVIRON CONTAM TOX, V76, P512, DOI 10.1007/s00128-006-0950-x; Whitman SM, 2001, ACAD PSYCHIATR, V25, P143, DOI 10.1176/appi.ap.25.3.143; ZIEGLER R, 1965, BEITR BIOL PFLANZ, V41, P11	25	72	72	0	80	AMER CHEMICAL SOC	WASHINGTON	1155 16TH ST, NW, WASHINGTON, DC 20036 USA	0003-2700	1520-6882		ANAL CHEM	Anal. Chem.	OCT 15	2007	79	20					7611	7627		10.1021/ac070236m			17	Chemistry, Analytical	Chemistry	220QW	WOS:000250173200004	17854156				2021-04-07	
J	Maranda, L; Corwin, S; Hargraves, PE				Maranda, Lucie; Corwin, Susannah; Hargraves, Paul E.			Prorocentrum lima (Dinophyceae) in northeastern USA coastal waters - I. Abundance and distribution	HARMFUL ALGAE			English	Article						diarrhetic shellfish poisoning; diarrhoeic shellfish poisoning; DSP; ectocarpus siliculosus; exuviaella lima; pilayella littoralis; Prorocentrum lima; tube-forming diatoms	POPULATIONS; GULF	The seasonal distribution of Prorocentrum lima within the epibiotic community associated with wild and cultured shellfish was studied at eight sites in northeastern USA coastal waters. This dinoflagellate produces toxins that can potentially accumulate in shellfish and lead to diarrhetic shellfish poisoning. Four sites in semi-sheltered environments provided sufficient data for an evaluation of population dynamics. The two southernmost populations (Point Judith Pond and Bluff Hill Cove in Rhode Island) displayed a double peak in abundance, one from March to June, with several thousands cells per g dry weight of collected epibiota, and a second one, sometimes minor, in autumn. At the two northern stations (New Meadows River and Clam Cove in Maine), P. lima populations also peaked twice but with a delay in timing. All four sites harbored filamentous seaweeds and aggregations of tube-forming or chains of diatoms, providing a favorable habitat for this epiphytic dinoflagellate. (C) 2007 Elsevier B.V. All rights reserved.	Univ Rhode Isl, Grad Sch Oceanog, Narragansett, RI 02882 USA	Maranda, L (corresponding author), Univ Rhode Isl, Grad Sch Oceanog, Narragansett, RI 02882 USA.	lmaranda@gso.uri.edu					Bravo I, 2001, TOXICON, V39, P1537, DOI 10.1016/S0041-0101(01)00126-X; Cembella A.D., 1989, Journal of Applied Phycology, V1, P307, DOI 10.1007/BF00003466; Cienkowski L., 1881, SANKTPETERBURGSKOE O, V12, P130; FAUST MA, 1993, DEV MAR BIO, V3, P121; FAUST MA, 1991, J PHYCOL, V27, P642, DOI 10.1111/j.0022-3646.1991.00642.x; FAUST MA, 1990, TOXIC MARINE PHYTOPLANKTON, P138; Foden J, 2005, HARMFUL ALGAE, V4, P1063, DOI 10.1016/j.hal.2005.03.004; FREUDENTHAL AR, 1985, 3RD P INT C TOX DIN, P461; Hargraves PE, 2002, NORTHEAST NAT, V9, P81, DOI 10.1656/1092-6194(2002)009[0081:PTOHMF]2.0.CO;2; JACKSON AE, 1993, DEV MAR BIO, V3, P513; Lawrence JE, 2000, MAR ECOL PROG SER, V201, P147, DOI 10.3354/meps201147; Lee JS, 1989, J APPL PHYCOL, V1, P147, DOI 10.1007/BF00003877; Levasseur M, 2003, AQUAT MICROB ECOL, V30, P283, DOI 10.3354/ame030283; LOBEL PS, 1988, BIOL BULL, V175, P94, DOI 10.2307/1541896; MARANDA L, 1987, ESTUARIES, V10, P298, DOI 10.2307/1351887; Maranda L, 1999, J PHYCOL, V35, P1158, DOI 10.1046/j.1529-8817.1999.3561158.x; Maranda L, 2000, J SHELLFISH RES, V19, P1003; Maranda L, 2007, HARMFUL ALGAE, V6, P632, DOI 10.1016/j.hal.2006.12.006; McLachlan J. L., 1994, Natural Toxins, V2, P263, DOI 10.1002/nt.2620020504; Mclachlan JL, 1997, PHYCOLOGIA, V36, P38, DOI 10.2216/i0031-8884-36-1-38.1; Morton SL, 1999, J SHELLFISH RES, V18, P681; QUILLIAM MA, 1993, DEV MAR BIO, V3, P547; Steidinger K.A., 1983, Progress phycol. Res., V2, P147; Tindall Donald R., 1998, NATO ASI Series Series G Ecological Sciences, V41, P293	24	14	16	0	8	ELSEVIER	AMSTERDAM	RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS	1568-9883	1878-1470		HARMFUL ALGAE	Harmful Algae	OCT	2007	6	5					623	631		10.1016/j.hal.2006.12.007			9	Marine & Freshwater Biology	Marine & Freshwater Biology	213QG	WOS:000249681600001					2021-04-07	
J	McCauley, LAR; Wehr, JD				McCauley, Linda A. R.; Wehr, John D.			Taxonomic reappraisal of the freshwater brown algae Bodanefla, Ectocarpus, Heribaudiella, and Pleurocladia (Phaeophyceae) on the basis of rbcL sequences and morphological characters	PHYCOLOGIA			English	Article						Bodanella; brown algae; Ectocarpales; Ectocarpus; freshwater; Heribaudiella; Phaeophyceae; phylogeny; Pleurocladia; rbcL; RUBTSCO	RUBISCO LARGE SUBUNIT; PHYLOGENETIC-RELATIONSHIPS; MOLECULAR PHYLOGENY; SP-NOV; SYSTEMATIC POSITION; ORDER ECTOCARPALES; SPHACELARIALES; ULTRASTRUCTURE; REASSESSMENT; PORTERINEMA	This study examines the phylogenetic relations among freshwater species of brown algae using RUBISCO large subunit (rbcL) sequences, in conjunction with diagnostic morphological and developmental traits. We focused on the phylogenetic and taxonomic placement of five freshwater species, Bodanella lauterborni, Ectocarpus siliculosus, Heribaudiella fluviatilis, Pleurocladia lacustris, and Porterinema fluviatile, each of which has traditionally been assigned to the Ectocarpales by most authors. These freshwater species were compared with 26 marine taxa of brown algae from nine orders, with particular emphasis on families within the Ectocarpales. Results of BLASTn searches and later phylogenetic analyses placed all freshwater taxa within the Phaeophyceae, but phylogenetic inferences do not support their traditional classifications at the ordinal level. There was no support for the inclusion of H. fluviatilis or B. lauterborni in the Ectocarpales, but instead formed a well-supported clade intermediate between the Syringodermatales and Sphacelariales. Although morphologies of these two species are relatively distinct and remain stable in culture, B. lauterborni nested between two populations of H. fluviatilis (on the basis of rbcL data) from British Columbia and Germany, making a reclassification of these two species necessary. Molecular and morphological data clearly place two other freshwater taxa, Pleurocladia lacustris and (freshwater) E. siliculosus in the Ectocarpales, each nesting among marine members of the Ectocarpaceae. When compared with sequences from 13 species from several families within the Ectocarpales, Pleurocladia is most closely related to members of the Ectocarpaceae and may be a sister taxon to the Scytosiphonaceae. DNA sequences from a freshwater isolate of E. siliculosus from Australia were nearly identical to those from a marine isolate from Germany. Molecular data did not resolve the ordinal status of a freshwater Porterinema fluviatile, but indicated that this species should not be classified within the Ectocarpales.	Fordham Univ, Dept Biol Sci, Louis Calder Ctr, Biol Field Stn, Armonk, NY 10504 USA	Wehr, JD (corresponding author), Fordham Univ, Dept Biol Sci, Louis Calder Ctr, Biol Field Stn, Armonk, NY 10504 USA.	wehr@fordham.edu		Wehr, John/0000-0003-4203-0921			ALLEN MM, 1968, J PHYCOL, V4, P1, DOI 10.1111/j.1529-8817.1968.tb04667.x; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Andersen RA, 1998, J PHYCOL, V34, P286, DOI 10.1046/j.1529-8817.1998.340286.x; ASSALI NE, 1990, PLANT MOL BIOL, V15, P307, DOI 10.1007/BF00036916; Bailey JC, 1998, PROTIST, V149, P245, DOI 10.1016/S1434-4610(98)70032-X; Bold H.C., 1978, INTRO ALGAE; BOURRELLY P, 1981, ALGUES EAU DOUCE, V2, P438; BRAUN A, 1855, ALGARUM UNICELLURARU, P1; Burrowes R, 2003, CRYPTOGAMIE ALGOL, V24, P63; Daugbjerg N, 1997, J PHYCOL, V33, P1031, DOI 10.1111/j.0022-3646.1997.01031.x; DEREVIERS B, 1999, PROGR PHYCOLOGICAL R, V13, P107; DOP AJ, 1979, ACTA BOT NEERL, V28, P449, DOI 10.1111/j.1438-8677.1979.tb01169.x; Draisma SGA, 2001, J PHYCOL, V37, P586, DOI 10.1046/j.1529-8817.2001.037004586.x; Draisma SGA, 2002, EUR J PHYCOL, V37, P385, DOI 10.1017/S0967026202003736; Ekenstam Devon, 1996, Journal of Phycology, V32, P15; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; GEISSLER U, 1983, NOVA HEDWIGIA, V37, P193; HENRY EC, 1984, PHYCOLOGIA, V23, P419, DOI 10.2216/i0031-8884-23-4-419.1; HENRY EC, 1987, PHYCOLOGIA, V26, P182, DOI 10.2216/i0031-8884-26-2-182.1; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; ISRAELSSON G, 1938, BOT NOTISER, P113; Jao Chin-chih, 1943, SINENSIA, V14, P151; Kawai H, 2005, PHYCOLOGIA, V44, P169, DOI 10.2216/0031-8884(2005)44[169:MAMPOP]2.0.CO;2; Kawai H, 2001, J PHYCOL, V37, P130, DOI 10.1046/j.1529-8817.1999.014012130.x; Kirkby SM, 1972, VASCULUM, V57, P51; KuselFetzmann EL, 1996, NOVA HEDWIGIA, V62, P79; MADDISON DR, 2001, MACCLADE 4 ANAL PHYL; MCCAULEY LAR, 2002, PHYLOGENETIC RELATIO; Muller DG, 1998, PHYCOLOGIA, V37, P425, DOI 10.2216/i0031-8884-37-6-425.1; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; Muller KM, 1998, PHYCOLOGIA, V37, P195, DOI 10.2216/i0031-8884-37-3-195.1; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; Pringsheim EG, 1946, PURE CULTURES ALGAE; PUESCHEL CM, 1983, J PHYCOL, V19, P209, DOI 10.1111/j.0022-3646.1983.00209.x; Rousse A, 2000, CR ACAD SCI IV-PHYS, V1, P305, DOI 10.1016/S1296-2147(00)00131-1; Rousseau F, 1999, CRYPTOGAMIE ALGOL, V20, P5, DOI 10.1016/S0181-1568(99)80002-6; Saunders GW, 1997, J PHYCOL, V33, P310, DOI 10.1111/j.0022-3646.1997.00310.x; SCHLOESSER RE, 1980, J PHYCOL, V16, P201; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; STARMACK K, 1977, FLORA SODKOWODNA POL, V14; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Swofford D.L., 2002, PAUP PHYLOGENTIC ANA; SZYMANSKA H, 1990, ARCH HYDROBIOL, P25; TAN IH, 1994, J PHYCOL, V30, P721, DOI 10.1111/j.0022-3646.1994.00721.x; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; WAERN MATS, 1952, ACTA PHYTO GEOGR SUECICA, V30, P1; Wehr JD, 2003, WEST N AM NATURALIST, V63, P517; WEHR JD, 1985, J PHYCOL, V21, P81; Wehr John D., 2003, P757, DOI 10.1016/B978-012741550-5/50023-4; WEST JA, 1996, ECTOVARPIS SILICULOS, V9, P29; WILCE RT, 1966, J PHYCOL, V2, P57, DOI 10.1111/j.1529-8817.1966.tb04595.x; WILCE RT, 1970, MAR BIOL, V5, P119, DOI 10.1007/BF00352595; ZIMMERMANN W, 1928, Z BOT, V20, P1	54	21	24	0	4	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0031-8884	2330-2968		PHYCOLOGIA	Phycologia	JUL	2007	46	4					429	439		10.2216/05-08.1			11	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	187NW	WOS:000247855900008					2021-04-07	
J	Park, Y; Kim, GD; Choi, TJ				Park, Yunjung; Kim, Gun-Do; Choi, Tae-Jin			Molecular cloning and characterization of the DNA adenine methyltransferase gene in Feldmannia sp virus	VIRUS GENES			English	Article						algal virus; Feldmannia; FsV; DNA methylation	SEQUENCE MOTIFS; ALGAL VIRUS; METHYLATION; RESTRICTION; INFECTION; SPECIFICITY; EXPRESSION; ALIGNMENT; MUTANTS; ENCODES	The genome of Feldmannia sp. virus (FsV), a marine brown alga virus, contains a putative DNA adenine methyltransferase (dam) gene of 1,245 bp that encodes a polypeptide of 45.8 kDa. A BLAST search with the FsV dam gene showed high amino acid identity to two putative methyltransferase genes, ORF B29 of Feldmannia irregularis virus (FirrV, 54%) and ORF129 of Ectocarpus siliculosus virus (EsV, 36%); and a PSI BLAST search revealed similarity to the N-6-adenine methyltransferases (MTases) of other species. Most conserved motifs of beta-class MTases were observed in the FsV dam gene. However, neither of the highly conserved sequences in motifs I (FxGxG) or IV [(S/N/D)PP(Y/F/W)] perfectly matched those in the FsV dam gene. The highly conserved DPPY consensus sequence in motif IV was NTPW in the FsV dam gene, perfectly matching the sequences in ORF B29 of FirrV and ORF129 of EsV. Therefore, the dam genes in brown algae viruses may belong to a yet undiscovered group. The FsV Dam protein expressed from the cloned FsV dam gene methylated E. coli chromosomal DNA. This is the first report showing that a virus infecting marine filamentous brown algae encodes a functional Dam protein.	Pukyong Natl Univ, Dept Microbiol, Pusan 608737, South Korea	Choi, TJ (corresponding author), Pukyong Natl Univ, Dept Microbiol, 599-1 Daeyeong 3 Dong, Pusan 608737, South Korea.	choitj@pknu.ac.kr					ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Baranyi U, 2000, MOL MICROBIOL, V35, P1168, DOI 10.1046/j.1365-2958.2000.01786.x; BARLOW DP, 1995, SCIENCE, V270, P1610, DOI 10.1126/science.270.5242.1610; BARRAS F, 1989, TRENDS GENET, V5, P139, DOI 10.1016/0168-9525(89)90054-1; BICKLE TA, 1993, MICROBIOL REV, V57, P434, DOI 10.1128/MMBR.57.2.434-450.1993; BROMBERG S, 1982, J BACTERIOL, V150, P993, DOI 10.1128/JB.150.2.993-996.1982; Bujnicki JM, 2002, BMC EVOL BIOL, V2, DOI 10.1186/1471-2148-2-3; Bujnicki JM, 2002, J MOL EVOL, V55, P431, DOI 10.1007/s00239-002-2339-8; CEDAR H, 1988, CELL, V53, P3, DOI 10.1016/0092-8674(88)90479-5; Doerfler W, 2005, BIOCHEMISTRY-MOSCOW+, V70, P505, DOI 10.1007/s10541-005-0145-9; DOERFLER W, 1983, ANNU REV BIOCHEM, V52, P93, DOI 10.1146/annurev.bi.52.070183.000521; Fedoreyeva LI, 2002, FEBS LETT, V514, P305, DOI 10.1016/S0014-5793(02)02384-0; GRAHAM MW, 1995, TRANSGENIC RES, V4, P324, DOI 10.1007/BF01972529; Hattman S, 2005, BIOCHEMISTRY-MOSCOW+, V70, P550, DOI 10.1007/s10541-005-0148-6; HATTMAN S, 1978, J MOL BIOL, V126, P367, DOI 10.1016/0022-2836(78)90046-3; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; HIROAKI S, 1996, GENE, V168, P37; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Kang S, 1999, J BIOL CHEM, V274, P11463, DOI 10.1074/jbc.274.17.11463; KLIMASAUSKAS S, 1989, NUCLEIC ACIDS RES, V17, P9823, DOI 10.1093/nar/17.23.9823; Kossykh VG, 1997, J BACTERIOL, V179, P3239, DOI 10.1128/jb.179.10.3239-3243.1997; KRUGER DH, 1983, MICROBIOL REV, V47, P345; Laird PW, 1996, ANNU REV GENET, V30, P441, DOI 10.1146/annurev.genet.30.1.441; Lee AM, 1998, J PHYCOL, V34, P608, DOI 10.1046/j.1529-8817.1998.340608.x; Lobner-Olesen A, 2005, CURR OPIN MICROBIOL, V8, P154, DOI 10.1016/j.mib.2005.02.009; Magrini V, 1997, J BACTERIOL, V179, P4254, DOI 10.1128/jb.179.13.4254-4263.1997; MALONE T, 1995, J MOL BIOL, V253, P618, DOI 10.1006/jmbi.1995.0577; MODRICH P, 1987, ANNU REV BIOCHEM, V56, P435, DOI 10.1146/annurev.bi.56.070187.002251; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Pan SH, 2000, BIOTECHNIQUES, V29, P1234, DOI 10.2144/00296st03; RAZIN A, 1991, MICROBIOL REV, V55, P451, DOI 10.1128/MMBR.55.3.451-458.1991; ROGERS SD, 1986, CURR GENET, V10, P557, DOI 10.1007/BF00447390; Sambrook J., 2001, MOL CLONING LAB MANU, V4th; SMITH HO, 1990, P NATL ACAD SCI USA, V87, P826, DOI 10.1073/pnas.87.2.826; STEFAN C, 1991, NUCLEIC ACIDS RES, V19, P307, DOI 10.1093/nar/19.2.307; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; van Blokland R, 1998, PLANT J, V15, P543, DOI 10.1046/j.1365-313X.1998.00238.x; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; WILSON GG, 1991, ANNU REV GENET, V25, P585, DOI 10.1146/annurev.ge.25.120191.003101; XIA YN, 1986, MOL CELL BIOL, V6, P1430, DOI 10.1128/MCB.6.5.1430; XIA YN, 1986, MOL CELL BIOL, V6, P1440, DOI 10.1128/MCB.6.5.1440; ZHANG YP, 1992, NUCLEIC ACIDS RES, V20, P5351, DOI 10.1093/nar/20.20.5351; Zhang YP, 1998, VIROLOGY, V240, P366, DOI 10.1006/viro.1997.8936; ZHU CM, 1990, BIOCHEM CELL BIOL, V68, P944, DOI 10.1139/o90-139	45	5	5	0	2	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0920-8569	1572-994X		VIRUS GENES	Virus Genes	APR	2007	34	2					177	183		10.1007/s11262-006-0059-7			7	Genetics & Heredity; Virology	Genetics & Heredity; Virology	145UK	WOS:000244890700010	17180708				2021-04-07	
J	Tuney, I; Cadirci, BH; Unal, D; Sukatar, A				Tuney, Inci; Cadirci, Bilge Hilal; Unal, Dilek; Sukatar, Atakan			Locational and organic solvent variation in antimicrobial activities of crude extracts of marine algae from the coast of Izmir (Turkey)	FRESENIUS ENVIRONMENTAL BULLETIN			English	Article						antimicrobial activity; macro-algae; algal extract	BROWN-ALGAE; SUBSTANCES	During this research, a total of 98 extracts from 13 algal species were tested in vitro for antimicrobial activities of algae against Candida sp., Enterococcus faecalis, Staphylococcus aureus, Streptococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli using the disc diffusion method. The ability of algae to perform antibacterial and antifungal activity was investigated at three different localities. Cystoseira mediterranea and Ulva rigida were the only algae found in all three sites, and contamination was detected in algae collected from sites I and 3, with antibacterial capacity being highest in those from site 1, where samples were collected during red tide. Extractions were performed with four different solvents - ethanol, methanol, acetone and diethyl ether. In particular, diethyl ether extracts of fresh C mediterranea, Enteromorpha linza, U. rigida, Gracilaria gracilis and Ectocarpus siliculosus appeared to yield better results than those of methanol, ethanol and acetone. However, diethyl ether extracts of some species, such as Padina pavonica, Colpomenia sniosa, Dictyota linearis, Dictyopteris membranacea, Ceramium rubrum, and Acanthophora nojadiformis, gave different results. Methanol extracts of A. nojadiformis showed effective antibacterial activity against P. aeruginosa, and methanol extracts of C rubrum showed antifungal and antibacterial activity against Candida sp., E. coli, and P. aeruginosa. Acetone extracts of Hypnea sp. showed antibacterial activity against P. aeruginosa, and ethanol extract of Cladophora sp. against E. faecalis only. Some of the collected samples were dried and extracts of fresh and dry samples were compared. Although fresh extracts of G. gracilis and E. siliculosus inhibited the tested microorganisms from populating, their dried extracts had no effect on gram-negative and positive bacteria.	Ege Univ, Fac Sci, Dept Biol, TR-35100 Izmir, Turkey	Unal, D (corresponding author), Ege Univ, Fac Sci, Dept Biol, TR-35100 Izmir, Turkey.	dilek.unal@mail.ege.edu.tr	CADIRCI, Bilge/AAA-9642-2021; Tuney, Inci/H-6531-2013				BOROWITZKA M. A., 1992, MICROALGAL BIOTECHNO, P179; del Val Antonio Gonzalez, 2001, International Microbiology, V4, P35; Febles C.I., 1995, ANUARIO I ESTUDIOS C, V34, P181; Haliki A, 2005, SU URUN DERG, V22, P13; KORAY T, 1984, EU FAC SCI J B, V1, P75; Lima JVM, 2002, BRAZ J MICROBIOL, V33, P311, DOI 10.1590/S1517-83822002000400006; Marechal JP, 2004, J EXP MAR BIOL ECOL, V313, P47, DOI 10.1016/j.jembe.2004.07.016; Masuda M, 1997, J PHYCOL, V33, P196, DOI 10.1111/j.0022-3646.1997.00196.x; MOREAU J, 1988, HYDROBIOLOGIA, V162, P157, DOI 10.1007/BF00014538; Norris J. N., 1985, HDB PHYCOLOGICAL MET, P121; PEREZ RM, 1990, J ETHNOPHARMACOL, V29, P111, DOI 10.1016/0378-8741(90)90104-2; ROSELL KG, 1987, HYDROBIOLOGIA, V151, P471, DOI 10.1007/BF00046169; SASTRY VMVS, 1994, BOT MAR, V37, P357, DOI 10.1515/botm.1994.37.4.357	13	14	18	0	16	PARLAR SCIENTIFIC PUBLICATIONS (P S P)	FREISING	ANGERSTR. 12, 85354 FREISING, GERMANY	1018-4619			FRESEN ENVIRON BULL	Fresenius Environ. Bull.		2007	16	4					428	434					7	Environmental Sciences	Environmental Sciences & Ecology	157ZF	WOS:000245759500015					2021-04-07	
J	Gachon, CMM; Kupper, H; Kupper, FC; Setlik, I				Gachon, Claire M. M.; Kupper, Hendrik; Kupper, Frithjof C.; Setlik, Ivan			Single-cell chlorophyll fluorescence kinetic microscopy of Pylaiella littoralis (Phaeophyceae) infected by Chytridium polysiphoniae (Chytridiomycota)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						biotrophy; chlorophyll fluorescence imaging; Chytridium; Phaeophyceae; photosynthesis; Pylaiella	ECTOCARPUS-SILICULOSUS ECTOCARPALES; MARINE BROWN-ALGAE; HIGH-LIGHT STRESS; PHOTOSYNTHETIC PERFORMANCE; XANTHOPHYLL-CYCLE; QUANTUM YIELD; LIFE-HISTORY; PHOTOPROTECTION; PLANTS; PHOTOINHIBITION	Chlorophyll fluorescence imaging is a non-invasive method to monitor the metabolic state of photosynthetic organisms. We used spatially resolved (imaging) microscopic measurements of chlorophyll fluorescence kinetics to follow the fate of individual cells of the filamentous brown alga Pylaiella littoralis infected by the biotrophic parasite Chytridium polysiphoniae (Chytridiomycota). These measurements showed strong differences between individual parameters of the inhibition of photosynthesis, revealing important details about the mechanism of damage. The dark-adapted photochemistry of photosystem II itself (measured as F-v/F-m, where F-v = F-m - F-0) remained unaffected until a very late stage of damage to the cell, while the light-adapted efficiency of PSII electron transport decreased earlier. A particularly complex pattern was found for the changes in nonphotochemical quenching (NPQ). The shape of the fluorescence transients suggests that the changes in NPQ during the actinic light period are caused by changes in F-0. The infection affected NPQ directly after the onset of the actinic light period more than in the steady state of photosynthesis. These results indicate that the infection affects the regulation of energy dissipation (e.g. by changes in antenna coupling). In early infection stages, NPQ increased, which is reflected by an increase in the 'vitality parameter' (relative fluorescence decrease, defined as (F-p - F-s)/F-s). In the second half of the infection process, all photosynthetic parameters declined, including the efficiency of photosystem II as measured by F-v/F-m, and NPQ.	Ecole Normale Super, F-75005 Paris, France; Scottish Assoc Marine Sci, Dunstaffnage Marine Lab, Oban PA37 1QA, Argyll, Scotland; Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany; Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic; Univ S Bohemia, Inst Phys Biol, CZ-37005 Ceske Budejovice, Czech Republic; Acad Sci Czech Republ, Inst Microbiol, CS-37981 Trebon, Czech Republic	Gachon, CMM (corresponding author), Ecole Normale Super, 45 Rue Ulm, F-75005 Paris, France.	claire.gachon@sams.ac.uk	Kupper, Hendrik/J-5152-2012; Gachon, Claire/C-2787-2009	Kupper, Hendrik/0000-0003-0712-7023; Gachon, Claire/0000-0002-3702-7472; Kuepper, Frithjof/0000-0003-1273-7109			ARSALANE W, 1994, PHOTOCHEM PHOTOBIOL, V60, P237, DOI 10.1111/j.1751-1097.1994.tb05097.x; Baker NR, 2004, J EXP BOT, V55, P1607, DOI 10.1093/jxb/erh196; Balachandran S, 1997, PHYSIOL PLANTARUM, V100, P203, DOI 10.1034/j.1399-3054.1997.1000201.x; Bartak M, 2004, PLANT BIOLOGY, V6, P333, DOI 10.1055/s-2004-820877; BILGER W, 1990, PHOTOSYNTH RES, V25, P161, DOI 10.1007/BF00033158; BUCHEL C, 1993, PHOTOCHEM PHOTOBIOL, V58, P137, DOI 10.1111/j.1751-1097.1993.tb04915.x; Chaerle L, 2004, PLANT CELL PHYSIOL, V45, P887, DOI 10.1093/pcp/pch097; Chou HM, 2000, MOL PLANT PATHOL, V1, P99, DOI 10.1046/j.1364-3703.2000.00013.x; Del Campo E, 1997, PHYCOLOGIA, V36, P186, DOI 10.2216/i0031-8884-36-3-186.1; Endo R, 2004, ENVIRON SCI TECHNOL, V38, P4165, DOI 10.1021/es035375+; Ferimazova N, 2002, PHOTOCHEM PHOTOBIOL, V76, P501, DOI 10.1562/0031-8655(2002)076&lt;0501:NIIPOR&gt;2.0.CO;2; GENTY B, 1989, BIOCHIM BIOPHYS ACTA, V990, P87, DOI 10.1016/S0304-4165(89)80016-9; GOVINDJEE, 1995, AUST J PLANT PHYSIOL, V22, P131, DOI 10.1071/PP9950131; Hanelt D, 1997, J PHYCOL, V33, P387, DOI 10.1111/j.0022-3646.1997.00387.x; Harker M, 1999, EUR J PHYCOL, V34, P35, DOI 10.1017/S0967026299001924; Ivanov B, 2000, PLANTA, V210, P765, DOI 10.1007/s004250050678; Juneau P, 2003, AQUAT MICROB ECOL, V31, P9, DOI 10.3354/ame031009; Karsten U, 2001, OECOLOGIA, V127, P11, DOI 10.1007/s004420000553; KRAUSE GH, 1991, ANNU REV PLANT PHYS, V42, P313, DOI 10.1146/annurev.pp.42.060191.001525; Kupper FC, 2006, CRYPTOGAMIE ALGOL, V27, P165; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; Kupper H, 2000, PHOTOSYNTHETICA, V38, P553, DOI 10.1023/A:1012461407557; Lavaud J, 2004, J PHYCOL, V40, P130, DOI 10.1046/j.1529-8817.2004.03026.x; LICHTENTHALER HK, 1988, CRC CR REV ANAL CHEM, V19, pS29, DOI 10.1080/15476510.1988.10401466; Maier I, 2000, PROTIST, V151, P225, DOI 10.1078/1434-4610-00021; Maxwell K, 2000, J EXP BOT, V51, P659, DOI 10.1093/jexbot/51.345.659; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; MULLER DG, 1989, BOT MAR, V32, P71, DOI 10.1515/botm.1989.32.1.71; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Nedbal L, 2000, PHOTOSYNTH RES, V66, P3, DOI 10.1023/A:1010729821876; Noctor G, 1998, ANNU REV PLANT PHYS, V49, P249, DOI 10.1146/annurev.arplant.49.1.249; Oxborough K, 2004, J EXP BOT, V55, P1195, DOI 10.1093/jxb/erh145; Panstruga R, 2003, CURR OPIN PLANT BIOL, V6, P320, DOI 10.1016/S1369-5266(03)00043-8; Pearson G, 2000, MAR ECOL PROG SER, V202, P67, DOI 10.3354/meps202067; Peters AF, 2004, J PHYCOL, V40, P1079, DOI 10.1111/j.1529-8817.2004.04058.x; ROBLEDO DR, 1994, EUR J PHYCOL, V29, P247, DOI 10.1080/09670269400650701; Rohacek K, 2002, PHOTOSYNTHETICA, V40, P13, DOI 10.1023/A:1020125719386; ROLFE SA, 1995, NEW PHYTOL, V131, P69, DOI 10.1111/j.1469-8137.1995.tb03056.x; Schofield O, 1998, J PHYCOL, V34, P104, DOI 10.1046/j.1529-8817.1998.340104.x; Scholes JD, 1996, PLANTA, V199, P573, DOI 10.1007/BF00195189; SCHREIBER U, 1993, PHOTOSYNTH RES, V36, P65, DOI 10.1007/BF00018076; SCHREIBER U, 1995, AUST J PLANT PHYSIOL, V22, P209, DOI 10.1071/PP9950209; SPARROW F. K., 1934, DANSK BOT ARKIV, V8, P1; STARR R, 1993, J PHYCOL, V29, pS1; VANKOOTEN O, 1990, PHOTOSYNTH RES, V25, P147, DOI 10.1007/BF00033156	46	14	16	0	11	TAYLOR & FRANCIS LTD	ABINGDON	4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	NOV	2006	41	4					395	403		10.1080/09670260600960918			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	131AO	WOS:000243840500003		Bronze			2021-04-07	
J	Maida, M; Sammarco, PW; Coll, JC				Maida, Mauro; Sammarco, Paul W.; Coll, John C.			A diffusion chamber for assessing efficacy of natural anti-fouling defenses in marine organisms	JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY			English	Article						Alcyonacea; anti-fouling; bio-fouling; chamber; diatoms; diffusion; Ectocarpus; Octocorallia; Sinularia flexibilis; soft coral	CORAL SINULARIA-FLEXIBILIS; CHEMICAL ECOLOGY; SOFT CORALS; SCLERACTINIAN CORALS; OCTOCORALLIA; INHIBITION; DITERPENES; SETTLEMENT; COELENTERATA; RECRUITMENT	A slow-release diffusion chamber is here described for use in assessing the effectiveness of potentially bioactive compounds as anti-fouling agents. Its settlement surface is a porous 15 mm thick slab of Portites lobata skeleton, through which an aqueous extract of a given marine organism is permitted to diffuse. The chamber is placed at an underwater site for 30 days at a time, and settlement of sessile, benthic organisms, particularly algae, is permitted to occur. Comparative settlement levels were compared between chambers containing an extract and a seawater control. An extract of Sinularia flexibilis (Octocorallia, Alcyonacea) was assessed using such chambers and found to be an effective natural anti-fouling agent or defense mechanism against Ectocarpus sp. and pennate diatoms, reducing their cover by similar to 50% when compared to experimental conditions using seawater as a control. Intra-chamber variability in extract concentration was found to be minimal. No significant differences were found in extract concentrations between replicate chambers through time. The chamber is a highly cost-effective and efficient tool by which such assessments can be made and easily replicated. It is recommended that coral which has recently died be collected and used as a source of substratum for this purpose. (c) 2006 Elsevier B.V. All rights reserved.	Louisiana Univ Marine Consortium, Chauvin, LA 70344 USA; Chancellory Australian Catholic Univ, Sydney, NSW 2060, Australia; IBAMA, CEPENE, BR-55578000 Pernambuco, Bahia, Brazil	Sammarco, PW (corresponding author), Louisiana Univ Marine Consortium, 8124 Hwy 56 Chauvin, Chauvin, LA 70344 USA.	mmaida@ibama.gov.br; psammarco@lumcon.edu; john.coll@bigpond.com					ACERET TL, 1995, MAR BIOL, V122, P317; Aceret TL, 1998, COMP BIOCHEM PHYS C, V120, P121, DOI 10.1016/S0742-8413(98)00032-2; ACERET TL, 1995, J EXP MAR BIOL ECOL, V188, P63, DOI 10.1016/0022-0981(94)00186-H; BAKUS GJ, 1986, J CHEM ECOL, V12, P951, DOI 10.1007/BF01638991; COHEN AL, 2004, PALEOCEANOGRAPHY, V19; COLL JC, 1982, J EXP MAR BIOL ECOL, V60, P293, DOI 10.1016/0022-0981(82)90166-6; COLL JC, 1987, MAR BIOL, V96, P129, DOI 10.1007/BF00394846; Davis A.R., 1989, Bioorganic Marine Chemistry, V3, P85; DAVIS AR, 1990, J CHEM ECOL, V16, P1349, DOI 10.1007/BF01021031; DAVIS AR, 1991, MAR ECOL PROG SER, V72, P117, DOI 10.3354/meps072117; Dobretsov S, 2005, MAR ECOL PROG SER, V297, P119, DOI 10.3354/meps297119; FENDLER E, 1985, P 5 INT COR REEF C T, V3, P295; FLEURY BG, IN PRESS MAR ECOL; GERHART DJ, 1988, J CHEM ECOL, V14, P1905, DOI 10.1007/BF01013485; MAIDA M, 1995, MAR ECOL PROG SER, V121, P191, DOI 10.3354/meps121191; MAIDA M, 1995, B MAR SCI, V56, P303; Matthee GF, 1998, J NAT PROD, V61, P237, DOI 10.1021/np970458n; Michalek K, 1997, J CHEM ECOL, V23, P259, DOI 10.1023/B:JOEC.0000006358.47272.56; NAKATSU T, 1983, EXPERIENTIA, V39, P759, DOI 10.1007/BF01990312; NYLUND GM, 1983, MAR ECOL-PROG SER, V299, P111; PHILLIPS DW, 1982, J EXP MAR BIOL ECOL, V58, P285, DOI 10.1016/0022-0981(82)90135-6; RITSCHOFF D, 1985, J CHEM ECOL, V11, P551; SAMARCO PW, 1996, P 8 INT COR REEF C P, V2, P1245; Sammarco P.W., 1983, Coral Reefs, V1, P173, DOI 10.1007/BF00571194; Sammarco P.W., 1988, Bioorganic Marine Chemistry, V2, P87; Sammarco P W, 1983, TOXICON S, V21, P69; SAMMARCO PW, 1992, MAR ECOL PROG SER, V88, P93, DOI 10.3354/meps088093; SAMMARCO PW, 1988, SCIENCE, V239, P1422, DOI 10.1126/science.239.4846.1422; SAMMARCO PW, 1989, LIMNOL OCEANOGR, V34, P898; Sokal R.R., 1981, BIOMETRY; STANDING JD, 1984, J CHEM ECOL, V10, P823, DOI 10.1007/BF00987966; Steinberg PD, 2002, J CHEM ECOL, V28, P1935, DOI 10.1023/A:1020789625989; TARGETT NM, 1983, J CHEM ECOL, V9, P817, DOI 10.1007/BF00987807; THOMPSON JE, 1985, MAR BIOL, V88, P11, DOI 10.1007/BF00393038; WAHL M, 1989, MAR ECOL PROG SER, V58, P175, DOI 10.3354/meps058175; WALKER RP, 1985, MAR BIOL, V88, P27, DOI 10.1007/BF00393040; WEBB L, 1983, TOXICON S, V3, P485; WILLIAMS J, 1989, FLUID PHYS OCEANOGRA; Zar JH., 1984, BIOSTATISTICAL ANAL	39	16	18	0	10	ELSEVIER SCIENCE BV	AMSTERDAM	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS	0022-0981			J EXP MAR BIOL ECOL	J. Exp. Mar. Biol. Ecol.	SEP 19	2006	337	1					59	64		10.1016/j.jembe.2006.06.008			6	Ecology; Marine & Freshwater Biology	Environmental Sciences & Ecology; Marine & Freshwater Biology	081YO	WOS:000240353700007					2021-04-07	
J	Thomsen, MS; McGlathery, KJ; Tyler, AC				Thomsen, Mads Solgaard; McGlathery, Karen J.; Tyler, Anna Christina			Macroalgal distribution patterns in a shallow, soft-bottom lagoon, with emphasis on the nonnative Gracilaria vermiculophylla and Coldium fragile	ESTUARIES AND COASTS			English	Article							EELGRASS ZOSTERA-MARINA; CODIUM-FRAGILE; SSP TOMENTOSOIDES; SARGASSUM-MUTICUM; RHODE-ISLAND; DRIFT ALGAE; NITROGEN LIMITATION; HALIDRYS-SILIQUOSA; EUTROPHICATION; COMMUNITIES	We determined the distribution of macroalgae in Hog Island Bay, a shallow coastal lagoon in Virginia, USA, seasonally at 12 sites from 1998 to 2000 and at 3 representative sites from 2000 to 2002. We analyzed macroalgal biomass, taxonomic richness, and abundance of two non-native species, the cryptic invader Gracilaria venniculophylla and the conspicuous Codium fragile, with respect to season, location (mainland, mid lagoon, barrier island sites), and elevation (intertidal, subtidal). Taxonomic richness, total algal biomass, and normative biomass peaked in the summer months when temperature and light availability were highest. A few stress tolerant and ephemeral algae dominated the algal assemblage. G. vermniculophylla constituted 74% of the entire algal biomass, was the most abundant alga in all seasons, locations, and elevation levels, and was positively correlated with taxonomic richness and abundance of filamentous species. Ulva curvata, Bryopsis plumosa, and C.fragile accounted for an additional 16% of the algal biomass. There are distinct habitats in Hog Island Bay that can be classified into low diversity-low biomass regions near the mainland and barrier islands and high diversity-high biomass regions in the open mid lagoon, where abundant shells for attachment and intermediate levels of water column nutrients and turbidity likely create better growth conditions. Taxonomic richness and biomass were higher in subtidal than intertidal zones, presumably due to lower desiccation stress. This study provides an example of how a single invasive species can dominate an entire assemblage, both in terms of biomass (being most abundant in all seasons, locations, and tidal levels) and species richness (correlating positively with epiphytic filamentous taxa). By adding hard-substratum structural complexity to a relatively homogenous soft-substratum system, G. vermiculaphylla increases substratum availability for attachment and entanglement of other algal species and enhances local diversity. Without widespread and abundant G. vermiculophylla, taxa like Polysiphonia, Ceramiunt, Bryopsis, Ectocarpus, and Champia would likely be much less common. This study also highlights the importance of using DNA analysis of voucher specimens in monitoring programs to accurately identify cryptic invaders.	Univ Virginia, Dept Environm Sci, Charlottesville, VA 22903 USA	Thomsen, MS (corresponding author), Edith Cowan Univ, Sch Nat Sci, Fac Comp Hlth & Sci, Joondalup Campus,100 Joondalup Dr, Joondalup, WA 6027, Australia.	mads_thomsen@mailcity.com	Thomsen, Mads/B-1204-2011				BELL SS, 1982, J EXP MAR BIOL ECOL, V61, P175, DOI 10.1016/0022-0981(82)90007-7; Bellorin AM, 2004, PHYCOL RES, V52, P69, DOI 10.1111/j.1440-183.2004.00330.x; BORUM J, 1985, MAR BIOL, V87, P211, DOI 10.1007/BF00539431; Boynton WR, 1996, ESTUARIES, V19, P408, DOI 10.2307/1352459; Bruno JF, 2003, TRENDS ECOL EVOL, V18, P119, DOI 10.1016/S0169-5347(02)00045-9; Bruno JF, 2001, MARINE COMMUNITY ECOLOGY, P201; CANCINO J, 1981, 10 INT SEAW S, P241; CARLTON JT, 1985, BOT MAR, V28, P155, DOI 10.1515/botm.1985.28.4.155; CASTEL J., 1996, HYDROBIOLOGIA, V329, P9; Castilla JC, 2004, MAR ECOL PROG SER, V268, P119, DOI 10.3354/meps268119; CECERE E, 1992, J APPL PHYCOL, V4, P323, DOI 10.1007/BF02185789; CONNOR JL, 1980, BOT MAR, V23, P711; COWPER SW, 1978, CONTRIB MAR SCI, V21, P125; CROMWELL JE, 1971, BARRIER COAST DISTRI; DOTY MS, 1946, ECOLOGY, V27, P315, DOI 10.2307/1933542; DROMGOOLE FI, 1980, BOT MAR, V23, P149, DOI 10.1515/botm.1980.23.3.149; Fletcher R.L., 1996, MARINE BENTHIC VEGET, P7, DOI [DOI 10.1007/978-3-642-61398-2_2, 10.1007/978-3-642-61398-2_2]; Flindt M, 1997, ECOL MODEL, V102, P133, DOI 10.1016/S0304-3800(97)00093-8; Flindt MR, 1997, ECOL MODEL, V102, P143, DOI 10.1016/S0304-3800(97)00101-4; FRALICK R A, 1972, Phycologia, V11, P67, DOI 10.2216/i0031-8884-11-1-67.1; FRALICK RA, 1973, MAR BIOL, V19, P127, DOI 10.1007/BF00353583; GOSHORN DM, 2001, EXAMINATION BENTHIC; Gurgel CFD, 2004, J PHYCOL, V40, P748, DOI 10.1111/j.1529-8817.2004.03070.x; HANISAK MD, 1979, MAR BIOL, V50, P333, DOI 10.1007/BF00387010; HANISAK MD, 1979, MAR BIOL, V50, P319, DOI 10.1007/BF00387009; Hauxwell J, 2001, ECOLOGY, V82, P1007, DOI 10.1890/0012-9658(2001)082[1007:MCCTEZ]2.0.CO;2; HILLSON CJ, 1976, B TORREY BOT CLUB, V103, P266, DOI 10.2307/2484191; HOLMQUIST JG, 1994, J EXP MAR BIOL ECOL, V180, P235, DOI 10.1016/0022-0981(94)90069-8; Humm H. J, 1979, MARINE ALGAE VIRGINI; LAWSON S, 2003, THESIS U VIRGINIA CH; LEE V, 1985, ESTUARIES, V8, P191, DOI 10.2307/1352200; Lenihan HS, 1999, ECOL MONOGR, V69, P251, DOI 10.1890/0012-9615(1999)069[0251:PBCOOR]2.0.CO;2; LOWTHION D, 1985, MAR ENVIRON RES, V15, P263, DOI 10.1016/0141-1136(85)90005-4; MALINOWSKI KC, 1973, J PHYCOL, V9, P102, DOI 10.1111/j.1529-8817.1973.tb04063.x; MANGUM CP, 1968, MAR BIOL, V2, P33, DOI 10.1007/BF00351635; MCGLATHERY K, 2001, J PHYCOL, V35, P1; MCGLATHERY KJ, 1992, MAR ECOL PROG SER, V87, P173, DOI 10.3354/meps087173; McGlathery KJ, 2001, MAR ECOL PROG SER, V216, P1, DOI 10.3354/meps216001; Morand P, 1996, BOT MAR, V39, P491, DOI 10.1515/botm.1996.39.1-6.491; Norkko A, 1996, MAR ECOL PROG SER, V140, P141, DOI 10.3354/meps140141; Norkko A, 1998, J EXP MAR BIOL ECOL, V221, P99, DOI 10.1016/S0022-0981(97)00117-2; Norkko J, 2000, J EXP MAR BIOL ECOL, V248, P79, DOI 10.1016/S0022-0981(00)00155-6; Norton TA., 1983, PROGR PHYCOLOGICAL R, P333; Oertel GF, 2001, J COASTAL RES, V17, P775; ORTH RJ, 1984, AQUAT BOT, V18, P43, DOI 10.1016/0304-3770(84)90080-9; Pallant J., 2001, SPSS SURVIVAL GUIDE; Pedersen MF, 2005, AQUAT BOT, V83, P31, DOI 10.1016/j.aquabot.2005.05.004; PEDERSEN MF, 1995, OPHELIA, V41, P261, DOI 10.1080/00785236.1995.10422047; Raffaelli DG, 1998, OCEANOGR MAR BIOL<D>, V36, P97; REISE K, 1983, HELGOLANDER MEERESUN, V36, P151, DOI 10.1007/BF01983854; RHODES RG, 1970, CHESAPEAKE SCI, V11, P61; ROSINSKI JL, 2004, THESIS U VIRGINIA CH; Rueness J, 2005, PHYCOLOGIA, V44, P120, DOI 10.2216/0031-8884(2005)44[120:LHAMSO]2.0.CO;2; Ruiz GM, 1999, LIMNOL OCEANOGR, V44, P950, DOI 10.4319/lo.1999.44.3_part_2.0950; Ruiz GM, 1997, AM ZOOL, V37, P621; SANDJENSEN K, 1991, AQUAT BOT, V41, P137, DOI 10.1016/0304-3770(91)90042-4; Schneider C.W., 1991, SEAWEEDS SE US CAPE; Schwindt Evangelina, 2001, Biological Invasions, V3, P137, DOI 10.1023/A:1014571916818; SEARS JR, 1975, ECOL MONOGR, V45, P337, DOI 10.2307/1942411; Simberloff Daniel, 1999, Biological Invasions, V1, P21, DOI 10.1023/A:1010086329619; STANHOPE JW, 2003, THESIS VIRGINIA I MA; Tagliapietra D, 1998, ESTUAR COAST SHELF S, V47, P217, DOI 10.1006/ecss.1998.0340; TAYLOR D, 1995, MAR ECOL PROG SER, V127, P235, DOI 10.3354/meps127235; Thomsen MS, 2006, HELGOLAND MAR RES, V60, P50, DOI 10.1007/s10152-005-0016-1; Thomsen MS, 2006, J PHYCOL, V42, P139, DOI 10.1111/j.1529-8817.2006.00160.x; Thomsen MS, 2006, J EXP MAR BIOL ECOL, V328, P22, DOI 10.1016/j.jembe.2005.06.016; Thomsen MS, 2005, ESTUAR COAST SHELF S, V62, P63, DOI 10.1016/j.ecss.2004.08.007; Thomsen MS, 2004, AQUAT BOT, V80, P153, DOI 10.1016/j.aquabot.2004.08.002; THOMSEN MS, 2004, MACROALGAL DISTRIBUT; THORNEMILLER B, 1983, BOT MAR, V26, P231, DOI 10.1515/botm.1983.26.5.231; Trowbridge CD, 1998, OCEANOGR MAR BIOL<D>, V36, P1; Tyler AC, 2003, LIMNOL OCEANOGR, V48, P2125, DOI 10.4319/lo.2003.48.6.2125; Tyler AC, 2001, ESTUAR COAST SHELF S, V53, P155, DOI 10.1006/ecss.2001.0801; Underwood A.J., 1981, Oceanography and Marine Biology an Annual Review, V19, P513; VIRNSTEIN RW, 1985, AQUAT BOT, V23, P67, DOI 10.1016/0304-3770(85)90021-X; Wernberg T, 2004, HELGOLAND MAR RES, V58, P154, DOI 10.1007/s10152-004-0180-8; WOLFE JM, 1988, BOT MAR, V31, P525, DOI 10.1515/botm.1988.31.6.525; WOODIN SA, 1978, ECOLOGY, V59, P274, DOI 10.2307/1936373; WULFF B L, 1969, Chesapeake Science, V10, P29, DOI 10.2307/1351210; YAMAMOTO H, 1988, Bulletin of the Faculty of Fisheries Hokkaido University, V39, P1; Yokoya NS, 1999, HYDROBIOLOGIA, V399, P339; Zuhlke R, 2001, J SEA RES, V46, P261, DOI 10.1016/S1385-1101(01)00091-0	82	57	57	1	28	SPRINGER	NEW YORK	ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES	1559-2723	1559-2731		ESTUAR COAST	Estuaries Coasts	JUN	2006	29	3					465	473		10.1007/BF02784994			9	Environmental Sciences; Marine & Freshwater Biology	Environmental Sciences & Ecology; Marine & Freshwater Biology	075VC	WOS:000239912400011					2021-04-07	
J	Leonardi, PI; Miravalles, AB; Faugeron, S; Flores, V; Beltran, J; Correa, JA				Leonardi, PI; Miravalles, AB; Faugeron, S; Flores, V; Beltran, J; Correa, JA			Diversity, phenomenology and epidemiology of epiphytism in farmed Gracilaria chilensis (Rhodophyta) in northern Chile	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						epiphytism; Gracilaria chilensis; host; interface; mariculture; Rhodophyta	HARVEYELLA-MIRABILIS CRYPTONEMIALES; PARASITIC RED ALGA; SOUTHERN CHILE; FINE-STRUCTURE; LAMINARIOIDES RHODOPHYTA; HOLMSELLA-AUSTRALIS; CULTURE CONDITIONS; SP-NOV; HOST; INFECTION	This study identified the most common epiphytes infecting the algal host Gracilaria chilensis on a farm in northern Chile. Simultaneously, the types of host-epiphyte interfaces were characterized and their relative abundance and temporal variability were monitored. Five types of anatomical relationships were detected. Infection type I included the epiphytes weakly attached to the surface of the host and not associated with damage of host tissues (i.e. Hincksia mitchelliae, H. granulosa and Ectocarpus acutus). Infection type II included those epiphytes strongly attached to the surface of the host but not associated with any host tissue damage (i.e. Acrochaetium sp., Antithamnionella sp. and Colpomenia sinuosa). Infection type III included all the epiphytes that penetrated the outer layer of the host wall without damaging its cortical cells (i.e. Xenococcus sp. and Sahlingia subintegra). Infection type IV included epiphytes penetrating deep into the host cell wall, disorganizing the cortical tissue (i. e. Ulva lactuca and Acrosorium corallinarum). Infection type V included epiphytes that penetrated deeply into the cortex, reached the medullary tissue and caused destruction of the host's cells in the area around the infection (i.e. Ceramium rubrum and Polysiphonia harveyi). Prevalence varied with time and with infection type, with types II and III reaching up to 80% and 90% of the thalli respectively. Severity of epiphyte infection was similar to the distribution of infection prevalence, with crustose epiphytes colonizing up to 80% of the host surface.	Univ Nacl Sur, Dept Biol Bioquim & Farm, RA-8000 Bahia Blanca, Argentina; Pontificia Univ Catolica Chile, Ctr Adv Studies Ecol & Biodivers, Fac Ciencias Biol, LIA DIAMS, Santiago, Chile	Leonardi, PI (corresponding author), Univ Nacl Sur, Dept Biol Bioquim & Farm, RA-8000 Bahia Blanca, Argentina.	leonardi@uns.edu.ar		Faugeron, Sylvain/0000-0001-7258-5229			Agrios G.N., 1997, PLANT PATHOLOGY; APT K, 1989, DIS AQUAT ORGAN, V6, P151, DOI 10.3354/dao006151; APT KE, 1988, J PHYCOL, V24, P28; ARRONTES J, 1990, BOT MAR, V33, P205, DOI 10.1515/botm.1990.33.2.205; Bailey J. A., 1992, Colletotrichum: biology, pathology and control., P88; Buschmann AH, 1997, AQUACULTURE, V156, P269, DOI 10.1016/S0044-8486(97)00132-4; Buschmann AH, 1997, J APPL PHYCOL, V9, P129, DOI 10.1023/A:1007971615801; Buschmann AH, 2001, AQUACULTURE, V194, P203, DOI 10.1016/S0044-8486(00)00518-4; Buschmann Alejandro H., 1993, Hydrobiologia, V260-261, P345, DOI 10.1007/BF00049039; CALONGE FD, 1969, J EXP BOT, V20, P350, DOI 10.1093/jxb/20.2.350; CANCINO JM, 1987, HYDROBIOLOGIA, V151, P233, DOI 10.1007/BF00046135; COOPER RM, 1981, PLANT DIS CONTROL RE, P101; CORREA JA, 1993, J PHYCOL, V29, P853, DOI 10.1111/j.0022-3646.1993.00853.x; CORREA JA, 1994, EUR J PHYCOL, V29, P33, DOI 10.1080/09670269400650461; CORREA JA, 1990, THESIS DALHOUSIE U H; Dawes CJ, 2000, J APPL PHYCOL, V12, P567, DOI 10.1023/A:1026501613984; Ducker S.C., 1984, ENCY PLANT PHYSL, V17, P113; Evans L. V., 1978, MODERN APPROACHES TA, P87; EVANS LV, 1973, NEW PHYTOL, V72, P393, DOI 10.1111/j.1469-8137.1973.tb02047.x; Faugeron S, 2000, DIS AQUAT ORGAN, V42, P143, DOI 10.3354/dao042143; FLETCHER RL, 1995, J APPL PHYCOL, V7, P325, DOI 10.1007/BF00004006; FRIEDLANDER M, 1991, AQUAT BOT, V39, P315, DOI 10.1016/0304-3770(91)90006-Q; FRIEDLANDER M, 1992, BOT MAR, V35, P423, DOI 10.1515/botm.1992.35.5.423; GOFF L.J., 1982, PROGR PHYCOLOGICAL R, P289; GOFF LJ, 1976, J PHYCOL, V12, P313; GOFF LJ, 1976, CAN J BOT, V54, P268, DOI 10.1139/b76-025; GOFF LJ, 1979, J PHYCOL, V15, P87, DOI 10.1111/j.0022-3646.1979.00087.x; GOFF LJ, 1994, J PHYCOL, V30, P695, DOI 10.1111/j.0022-3646.1994.00695.x; GOFF LJ, 1984, P NATL ACAD SCI-BIOL, V81, P5420, DOI 10.1073/pnas.81.17.5420; GOFF LJ, 1985, J PHYCOL, V21, P483; GONZALEZ MA, 1989, J PHYCOL, V25, P558, DOI 10.1111/j.1529-8817.1989.tb00262.x; GONZALEZ MA, 1993, AQUACULTURE, V116, P135, DOI 10.1016/0044-8486(93)90004-I; Heesch Svenja, 1999, Phycological Research, V47, P1; KUGRENS P, 1982, AM J BOT, V69, P306, DOI 10.2307/2443018; KUSCHEL FA, 1991, AQUACULTURE, V92, P7, DOI 10.1016/0044-8486(91)90004-Q; LINSKENS H., 1963, PUBL STAZ ZOOL NAPOLI, V33, P274; LINSKENS HF, 1976, SPECIFICITY PLANT DI; MCKEEN WE, 1969, CAN J BOTANY, V47, P701, DOI 10.1139/b69-099; Mendgen K, 1996, ANNU REV PHYTOPATHOL, V34, P367, DOI 10.1146/annurev.phyto.34.1.367; PETERS AF, 1991, PHYCOLOGIA, V30, P365, DOI 10.2216/i0031-8884-30-4-365.1; Pizarro A., 1993, Hydrobiologia, V260-261, P357, DOI 10.1007/BF00049041; Pringle JD, 1989, J APPL PHYCOL, V1, P83; RAWLENCE D J, 1972, Phycologia, V11, P279, DOI 10.2216/i0031-8884-11-3-279.1; RAWLENCE DJ, 1972, J PHYCOL, V8, P15, DOI 10.1111/j.0022-3646.1972.00015.x; Retamales Claudia A., 1996, Hydrobiologia, V326-327, P355, DOI 10.1007/BF00047831; Svirski E., 1993, Hydrobiologia, V260-261, P391, DOI 10.1007/BF00049046; Taylor WS, 1985, MARINE ALGAE E TROPI; WETHERBEE R, 1982, PROTOPLASMA, V110, P166, DOI 10.1007/BF01283319; WETHERBEE R, 1982, PROTOPLASMA, V110, P153, DOI 10.1007/BF01283318; Wharton PS, 2001, PHYTOPATHOLOGY, V91, P149, DOI 10.1094/PHYTO.2001.91.2.149	50	44	47	1	17	TAYLOR & FRANCIS LTD	ABINGDON	4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	MAY	2006	41	2					247	257		10.1080/09670260600645659			11	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	053EE	WOS:000238286900011		Green Published			2021-04-07	
J	Allen, MJ; Schroeder, DC; Wilson, WH				Allen, MJ; Schroeder, DC; Wilson, WH			Preliminary characterisation of repeat families in the genome of EhV-86, a giant algal virus that infects the marine microalga Emiliania huxleyi	ARCHIVES OF VIROLOGY			English	Article							ECTOCARPUS-SILICULOSUS VIRUS; CALCIUM-BINDING PROTEIN; MOLECULAR-CLONING; DNA-REPLICATION; SEQUENCE; TRANSCRIPTION; ORIGIN; CELL	EhV-86 is a large double stranded DNA virus with a 407,339 base pair circular genome that infects the globally important microalga Emiliania huxleyi. It belongs to a new genus of viruses termed the Coccolithoviridae within the algal virus family Phycodnaviridae. By plotting the EhV-86 genome against itself in a dot-plot analysis we revealed three families of distinctly different repeat sequences throughout its genome, designated Family A, B and C. Family A repeats are non-coding, found immediately upstream of 86 predicted coding sequences (CDSs) and are likely to play a crucial role in controlling the expression of the associated CDSs. Family B repeats are GC rich, coding and correspond to possible calcium binding sites in 22 proline-rich domains found in the protein products of eight predicted EhV-86 CDSs. Family C repeats are AT-rich, non-coding and are likely to form part of the origin of replication. We suggest that these repeat regions are of fundamental importance during virus propagation being involved with transcriptional control (Family A), virus adsorption/release (Family B) and DNA replication (Family C).	Plymouth Marine Lab, Plymouth PL1 3DH, Devon, England; Marine Biol Assoc UK, Plymouth, Devon, England	Wilson, WH (corresponding author), Plymouth Marine Lab, Prospect Pl,The Hoe, Plymouth PL1 3DH, Devon, England.	whw@pml.ac.uk	Schroeder, Declan C./O-9131-2019; Allen, Michael J/C-1248-2011	Schroeder, Declan C./0000-0001-5991-2838; Allen, Michael J/0000-0001-8504-7171			ALLEN MJ, 2005, MOL BIOL EVOL; Blum H, 2001, VIROLOGY, V281, P6, DOI 10.1006/viro.2000.0776; Boehmer PE, 1997, ANNU REV BIOCHEM, V66, P347, DOI 10.1146/annurev.biochem.66.1.347; BROWNLEE C, 1994, HAPTOPHYTE ALGAE SYS, V51, P133; Chen Y, 2004, BIOCHEM BIOPH RES CO, V318, P1039, DOI 10.1016/j.bbrc.2004.04.136; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Endo H, 2000, BIOCHEM BIOPH RES CO, V276, P286, DOI 10.1006/bbrc.2000.3446; FLIEGEL L, 1989, J BIOL CHEM, V264, P21522; GALLI I, 1992, NUCLEIC ACIDS RES, V20, P3333, DOI 10.1093/nar/20.13.3333; GOMPELS UA, 1995, J GEN VIROL, V76, P451, DOI 10.1099/0022-1317-76-2-451; Hayakawa T, 2000, VIROLOGY, V278, P1, DOI 10.1006/viro.2000.0668; Hill CW, 1999, RES MICROBIOL, V150, P665, DOI 10.1016/S0923-2508(99)00125-4; Huang Y, 2004, BIOINFORMATICS, V20, P460, DOI 10.1093/bioinformatics/btg429; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; La Scola B, 2003, SCIENCE, V299, P2033; MARTIN JH, 1993, P NATL ACAD SCI USA, V90, P1531, DOI 10.1073/pnas.90.4.1531; Raoult D, 2004, SCIENCE, V306, P1344, DOI 10.1126/science.1101485; Ritzi M, 2003, J CELL SCI, V116, P3971, DOI 10.1242/jcs.00708; SCHLESINGER DH, 1986, INT J PEPT PROT RES, V27, P373; SCHUSTER AM, 1990, VIROLOGY, V176, P515, DOI 10.1016/0042-6822(90)90021-I; Stahl LE, 1996, J CELL SCI, V109, P1637; Sugden B, 2002, TRENDS BIOCHEM SCI, V27, P1, DOI 10.1016/S0968-0004(01)02032-1; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Vink C, 1996, J VIROL, V70, P5221, DOI 10.1128/JVI.70.8.5221-5229.1996; WESTBROEK P, 1984, PHILOS T ROY SOC B, V304, P435, DOI 10.1098/rstb.1984.0037; Wilson WH, 2005, SCIENCE, V309, P1090, DOI 10.1126/science.1113109; Wilson WH, 2002, J MAR BIOL ASSOC UK, V82, P369, DOI 10.1017/S002531540200560X; WILSON WH, 2005, VIRUS TAXONOMY, P163	30	17	18	0	6	SPRINGER WIEN	WIEN	SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA	0304-8608	1432-8798		ARCH VIROL	Arch. Virol.	MAR	2006	151	3					525	535		10.1007/s00705-005-0647-1			11	Virology	Virology	017CW	WOS:000235672700008	16195784				2021-04-07	
J	Raso, JE; Martin, MJ; Diaz, V; Cobos, V; Manjon-Cabeza, ME				Raso, JE; Martin, MJ; Diaz, V; Cobos, V; Manjon-Cabeza, ME			Diel and seasonal changes in the structure of a decapod (Crustacea : Decapoda) community of Cymodocea nodosa from southeastern spain (West mediterranean sea)	HYDROBIOLOGIA			English	Article; Proceedings Paper	8th Colloquium Crustacea Decapoda Mediterranea	SEP 02-06, 2002	Corfu Isl, GREECE			diel changes; decapod; seagrass; Cymodocea; diversity; species richness; Mediterranean Sea	FISH FAUNA; SEAGRASS MEADOWS; MACROBENTHOS; SELECTION; BOTTOMS; FLORIDA; RIVER	The study of a decapod community in a Cymodocea nodosa meadow from Southeastern Spain (Western Mediterranean Sea) showed a stable structure, in which the families Hippolytidae, Processidae, Majidae and Portunidae were the most abundant and the species Hippolyte niezabitowskii dominated. The animal community was more numerous and diverse during the night, showing the existence of nychthemeral movements, which are essentially related to the trophic behaviour and shelter. In this way, many species increased their abundance as a result of an increasing activity and, also, of an influx of other species and specimens from adjacent sandy bottoms, such as Processa spp. (mainly P. modica) Sicyonia carinata, Liocarcinus spp. (mainly juveniles) and several species of hermit crabs, which were rare or absent during the day. All these changes produced modifications in the dominance curves and in the values of all ecological indices (richness, diversity and evenness). Monthly samples were grouped and ordered (MDS) by the factor "day-night", which showed slight qualitative and quantitative differences (SIMPER, dissimilarity average of the factor day-night = 61.67). On the other hand, no global seasonal differences have been found (one way ANOSIM), but there was a significant level of similarity between winter and spring, while the summer samples were the most different. The differentiation of the summer 1999 can be attributed to a decrease in species abundance and richness, probably due to the dynamics of the decapod populations and the balance with predators (fishes), while that of the summer 2000, to an anomalous event: the massive proliferation of filamentous algae, mainly Ectocarpus s.l., which modified the environmental conditions.	Univ Malaga, Dept Anim Biol, Fac Ciencias, E-29071 Malaga, Spain	Raso, JE (corresponding author), Univ Malaga, Dept Anim Biol, Fac Ciencias, Campus Teatinos S-N, E-29071 Malaga, Spain.	garciaraso@uma.es	Manjon-Cabeza, M. Eugenia/L-1761-2014	Manjon-Cabeza, M. Eugenia/0000-0002-4014-6763			BELL CW, 1983, MUSCLE NONMUSCLE MOT, V2, P1; BELLANSANTINI D, 1994, BIOCENOSES MARINES L, V19; Chessa L. A., 1989, INT WORKSH POS OC BE, V2, P243; Clarke K.R., 1994, CHANGE MARINE COMMUN; COLES RG, 1993, AUST J MAR FRESH RES, V44, P193; CURRAS A, 1994, CAH BIOL MAR, V35, P91; de la Rosa IL, 2002, J MAR BIOL ASSOC UK, V82, P85, DOI 10.1017/S0025315402005209; Den Hartog C., 1977, SEAGRASS ECOSYSTEMS, P89; Garcia Raso JE., 1987, INVEST PESQ, V51, P301; GORE RH, 1981, ESTUAR COAST SHELF S, V12, P485, DOI 10.1016/S0302-3524(81)80007-2; GRAY JS, 1982, MAR ECOL PROG SER, V9, P111, DOI 10.3354/meps009111; Guidetti P, 2000, OCEANOL ACTA, V23, P759, DOI 10.1016/S0399-1784(00)01117-8; Hemminga M. A, 2000, SEAGRASS ECOLOGY; JACOBS RPWM, 1982, P K NED AKAD C BIOL, V85, P335; JACOBS RPWM, 1983, P K NED AKAD C BIOL, V86, P347; KIKUCHI T, 1977, SEAGRASS ECOSYSTEMS, P147; Krebs C. J., 1989, ECOLOGICAL METHODOLO; Ledoyer M., 1966, Recueil Trav Stn mar Endoume Bull, VNo. 41, P135; LEDOYER M, 1984, ZOOLOGISCHE VERHANDE, V211, P1; LEDOYER M, 1968, 4 S ET EC REC TRAV S, V60, P125; MAIN KL, 1987, ECOLOGY, V68, P170, DOI 10.2307/1938817; Matheson RE, 1999, ESTUARIES, V22, P534, DOI 10.2307/1353216; Mattila J, 1999, J SEA RES, V41, P321, DOI 10.1016/S1385-1101(99)00006-4; Moliner R., 1952, ANN, V27, P157; MOLINIER R, 1951, CR HEBD ACAD SCI, V233, P1212; PERES JM, 1964, REC TRAV STA MAR END, V47, P1; PIELOU E C, 1969, P286; RASO JEG, 1990, MARINE ECOLOGY, V11, P309; ROBERTSON AI, 1984, AQUAT BOT, V18, P135, DOI 10.1016/0304-3770(84)90084-6; Rooker JR, 1998, ESTUARIES, V21, P318, DOI 10.2307/1352478; Sanchez-Jerez P, 1999, ACTA OECOL, V20, P391, DOI 10.1016/S1146-609X(99)00128-9; Shannon C., 1963, MATH THEORY COMMUNIC; Stevcic Z., 1991, Acta Adriatica, V32, P637; Templado J., 1984, INT WORKSH POS OC BE, V1, P159; THAYER GW, 1984, USFWSOBS8402	35	23	23	0	5	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0018-8158	1573-5117		HYDROBIOLOGIA	Hydrobiologia	MAR	2006	557						59	68		10.1007/s10750-005-1308-9			10	Marine & Freshwater Biology	Marine & Freshwater Biology	999SK	WOS:000234410600009					2021-04-07	
J	Kato, Y; Kogame, K; Nagasato, C; Motomura, T				Kato, Y; Kogame, K; Nagasato, C; Motomura, T			Inheritance of mitochondrial and chloroplast genomes in the isogamous brown alga Scytosiphon lomentaria (Phaeophyceae)	PHYCOLOGICAL RESEARCH			English	Article						chloroplast; cox3; cytoplasmic inheritance; mitochondrion; rubisco; Scytosiphon lomentaria	ARTIFICIAL HYBRIDS; DNA; FERTILIZATION; GAMETOGENESIS; ELECTRON; SEAWEEDS; FUSION; ZYGOTE; SPERM; FATE	Patterns of inheritance of chloroplasts and mitochondria were examined by fluorescence microscopy and haplotype genome markers in the isogamous brown alga Scytosiphon lomentaria (Lyngbye) Link. Germination of the zygote in this species was unilateral, the growing thallus developed entirely from the germ tube, and the original zygote cell did not develop except for the formation of a hair. Inheritance of chloroplasts was biparental, and partitioning of the two parental chloroplasts into the first sporophytic cells was accidental: either the maternal or the paternal chloroplast was migrated from the zygote into the germ tube cell, whereas the other chloroplast remained in the original cell. In contrast, the mitochondrial genome in all cells of the sporophyte came only from the female gamete (maternal inheritance). These inheritance patterns are similar to those of the isogamous brown alga Ectocarpus siliculosus (Dillwyn) Lyngbye. Maternal inheritance of mitochondria might be universal in brown algae.	Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan; Hokkaido Univ, Field Sci Ctr No Biosphere, Muroran Marine Stn, Muroran, Hokkaido 0510003, Japan	Kogame, K (corresponding author), Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan.	kogame@sci.hokudai.ac.jp	Nagasato, Chikako/A-3392-2012		Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [17570047, 17570069] Funding Source: KAKEN		BIRKY CW, 1995, P NATL ACAD SCI USA, V92, P11331, DOI 10.1073/pnas.92.25.11331; BOUCK GB, 1970, ANN NY ACAD SCI, V175, P673, DOI 10.1111/j.1749-6632.1970.tb45184.x; BRAWLEY SH, 1976, J CELL SCI, V20, P255; COLEMAN AW, 1984, EXP CELL RES, V152, P528, DOI 10.1016/0014-4827(84)90655-4; Coyer JA, 2002, EUR J PHYCOL, V37, P173, DOI 10.1017/S0967026202003682; Kogame K, 1999, PHYCOLOGIA, V38, P496, DOI 10.2216/i0031-8884-38-6-496.1; Kogame K, 2005, EUR J PHYCOL, V40, P313, DOI 10.1080/09670260500193008; Kogame Yasuyo, 1996, Phycological Research, V44, P247, DOI 10.1111/j.1440-1835.1996.tb00054.x; Kraan S, 2000, PHYCOLOGIA, V39, P554, DOI 10.2216/i0031-8884-39-6-554.1; KUROIWA T, 1991, INT REV CYTOL, V128, P1, DOI 10.1016/S0074-7696(08)60496-9; KUROIWA T, 1986, PROTOPLASMA, V133, P85, DOI 10.1007/BF01293191; KUROIWA T, 1982, NATURE, V298, P481, DOI 10.1038/298481a0; Lee SH, 2002, J PHYCOL, V38, P534, DOI 10.1046/j.1529-8817.2002.t01-1-01212.x; Maier I, 1997, EUR J PHYCOL, V32, P241; MINE I, 1994, J PHYCOL, V30, P55, DOI 10.1111/j.0022-3646.1994.00055.x; MONGENSON HL, 1996, AM J BOT, V83, P383; MOTOMURA T, 1994, PROTOPLASMA, V178, P97, DOI 10.1007/BF01545960; MOTOMURA T, 1990, J PHYCOL, V26, P80, DOI 10.1111/j.0022-3646.1990.00080.x; Motomura Taizo, 1992, Japanese Journal of Phycology, V40, P207; Nagasato C, 2000, EUR J PHYCOL, V35, P339, DOI 10.1080/09670260010001735941; Nakamura S, 2003, PROTOPLASMA, V221, P205, DOI 10.1007/s00709-002-0053-4; NAKAMURA Y, 1975, SCI PAP I ALG RES FS, V6, P58; NISHIMURA Y, GENES DEV, V16, P1116; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; Swofford DL, 2002, PAUP PHYLOGENETIC AN; Tatewaki M, 1966, PHYCOLOGIA, V6, P62, DOI DOI 10.2216/I0031-8884-6-1-62.1; WAALAND SD, 1980, HDB PHYCOLOGICAL MET, P85; Zuccarello GC, 1999, HYDROBIOLOGIA, V401, P207, DOI 10.1023/A:1003706931897; Zuccarello GC, 1999, MOL ECOL, V8, P1443, DOI 10.1046/j.1365-294x.1999.00710.x	29	22	24	0	8	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	1322-0829	1440-1835		PHYCOL RES	Phycol. Res.	MAR	2006	54	1					65	71		10.1111/j.1440-1835.2006.00409.x			7	Marine & Freshwater Biology	Marine & Freshwater Biology	014GS	WOS:000235468200007					2021-04-07	
J	Cho, GY; Boo, SM				Cho, GY; Boo, SM			Phylogenetic position of Petrospongium rugosum (Ectocarpales, Phaeophyceae): insights from the protein-coding plastid rbcL and psaA gene sequences	CRYPTOGAMIE ALGOLOGIE			English	Article						Peirospongium rugosum; brown algae; molecular phylogeny; psaA; rbcL; pyrenoid; taxonomy; ultrastructure	RUBISCO LARGE SUBUNIT; ORDER; ADENOCYSTACEAE	The spongy, crustose brown alga Petrospongium rugosum (Okamura) Setchell et Gardner Occurs in Korea, Japan, Australia, New Zealand, and along the Pacific coast of North America. Although the species has been classified in the Chordariaceae of the Ectocarpales sensu lato or the family Leathesiaceae of the Chorclariales sensu stricto, the relationship of the Species to other brown algal lineages is less studied in terms of the plastid ultrastructure and molecular phylogeny. We examined the morphology of P rugosum and also determined protein-coding psaA and rbcL sequences from four samples of the species from different locations, comparing them with homologous positions of newly sequenced putative relatives (Leathesia difformis and Spermatochnus paradoxus) and with published sequences of other brown algae. The Species Occurs ill the upper intertidal zone oil the Korean south coast from November to June. Thalli are markedly rugose and are comprised of haplostichous filaments, arranged into cortical and medullary layers. Unilocular sporangia arise laterally oil the lower cells of cortical layers. A large pedunculate pyrenoid, with a cap, is present in the parietal discoid plastids. The specimens from four different locations were almost identical in rbcL and psaA sequences, and were monophyletic. All phylogenetic analyses of both genes reveal that P. rugosum is clearly separated from Leathesia and other members of the Chordariaceae. The sister relationship of the species to Ectocarpus was not Supported by bootstrap or Bayesian analyses.	Chungnam Natl Univ, Dept Biol, Taejon 305764, South Korea	Boo, SM (corresponding author), Chungnam Natl Univ, Dept Biol, Taejon 305764, South Korea.	smboo@cnu.ac.kr					Adams NM, 1994, SEAWEEDS NZ ILLUSTRA; Cho GY, 2004, J PHYCOL, V40, P921, DOI 10.1111/j.1529-8817.2004.03160.x; Cho TO, 2003, NOVA HEDWIGIA, V76, P381, DOI 10.1127/0029-5035/2003/0076-0381; Draisma S. G. A., 2003, OUT PAST COLLECTED R, P87; Draisma SGA, 2001, J PHYCOL, V37, P586, DOI 10.1046/j.1529-8817.2001.037004586.x; GILBERT DG, 1995, SEQPUP BIOL SEQUENCE; HORI T, 1971, BOT MAG TOKYO, V84, P231; Hori T., 1993, ILLUSTRATED ATLAS LI, V2; Huelsenbeck JP, 2001, BIOINFORMATICS, V17, P754, DOI 10.1093/bioinformatics/17.8.754; INAGAKI KI, 1958, SCI PAP I ALGOL RES, V4, P87; Kang JW., 1966, B PUSAN FISHERIES CO, V7, P1; Kogame K, 1999, PHYCOLOGIA, V38, P496, DOI 10.2216/i0031-8884-38-6-496.1; Kutzing F. T., 1858, TABULAE PHYCOLOGICAE, V8; MACLENNAN IG, 1956, P R SOC VICT, V69, P1; OKAMURA K, 2007, ICONES JAPANESE ALGA, V1; OKAMURA K, 1903, BOT MAG TOKYO, V17, P129; OLTMANNS F, 1922, MORPHOLOGIE BIOL ALG, V1; Peters AF, 2001, CRYPTOGAMIE ALGOL, V22, P187, DOI 10.1016/S0181-1568(01)01062-5; Posada D, 1998, BIOINFORMATICS, V14, P817, DOI 10.1093/bioinformatics/14.9.817; Reviers B., 1999, PROG PHYCOL RES, V13, P107; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; Rousseau F, 2001, CR ACAD SCI III-VIE, V324, P305, DOI 10.1016/S0764-4469(01)01306-3; Rousseau F, 1999, CRYPTOGAMIE ALGOL, V20, P5, DOI 10.1016/S0181-1568(99)80002-6; Rousseau F, 2000, EUR J PHYCOL, V35, P35, DOI 10.1017/S0967026200002638; SETCHELL WA, 1924, P CALIF ACAD SCI, V12, P659; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; Swofford DL, 2002, PAUP PHYLOGENETIC AN; Womersley H.B.S., 1987, MARINE BENTHIC FLORA; Yoon HS, 2002, P NATL ACAD SCI USA, V99, P11724, DOI 10.1073/pnas.172234799; Yoon HS, 1999, HYDROBIOLOGIA, V399, P47	31	3	4	0	0	ADAC-CRYPTOGAMIE	PARIS	12 RUE DE BUFFON, 75005 PARIS, FRANCE	0181-1568	1776-0984		CRYPTOGAMIE ALGOL	Cryptogam. Algol.	FEB	2006	27	1					3	15					13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	028YD	WOS:000236523800001					2021-04-07	
J	Dworjanyn, SA; de Nys, R; Steinberg, PD				Dworjanyn, S. A.; de Nys, R.; Steinberg, P. D.			Chemically mediated antifouling in the red alga Delisea pulchra	MARINE ECOLOGY PROGRESS SERIES			English	Article						antifouling; bioassay; chemical ecology; surface interaction; natural products; surface chemistry; furanones	KELP ECKLONIA-RADIATA; SECONDARY METABOLITES; HALOGENATED FURANONES; CORAL-REEF; HERBIVORES; PHLOROTANNINS; COEVOLUTION; EPIPHYTES; EXTRACTS; SEAWEEDS	Using laboratory assays, we tested whether the secondary metabolites (furanones) on the surface of the red alga Delisea pulchra deter the settlement and growth of a range of ecologically relevant fouling organisms. D. pulchra and 4 other co-occurring seaweeds were almost exclusively fouled by other algae. Consequently, we carried out laboratory assays using propagules from 4 fouling algae (Ulva sp., Ceramium sp., Polysiphonia sp. and Ectocarpus siliculosis) representing the natural fouling community. The crude surface extract of D. pulchra at the same concentration as on the surface of the plant, the furanone fraction of this extract, and pure furnaones, deterred the settlement of fouling organisms in ecologically relevant assays. These data, coupled with knowledge of the surface concentration of furanones on D. pulchra and a mechanism by which furanones are sequestered onto the surface of the plant, provide a rigorous demonstration of chemically mediated antifouling.	Univ New S Wales, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia; Univ New S Wales, Ctr Marine Biofouling & Bioinnovat, Sydney, NSW 2052, Australia	Dworjanyn, SA (corresponding author), Scottish Assoc Marine Sci, Dunstaffnage Marine Res Lab, Oban PA37 1QA, Argyll, Scotland.	symon.dworjanyn@sams.ac.uk	Dworjanyn, Symon A/O-5633-2017; de Nys, Rocky/D-6741-2012	Dworjanyn, Symon A/0000-0002-6690-8033; de Nys, Rocky/0000-0003-3869-4928			BERNARYS EA, 1994, HOST PLANT SELECTION, V2; BERNSTEIN BB, 1979, ECOL MONOGR, V49, P335, DOI 10.2307/1942488; Bold H. C., 1985, INTRO ALGAE STRUCTUR; Clare AS, 1996, BIOFOULING, V9, P211, DOI 10.1080/08927019609378304; DANTONIO C, 1985, J EXP MAR BIOL ECOL, V86, P197, DOI 10.1016/0022-0981(85)90103-0; Davis A.R., 1989, Bioorganic Marine Chemistry, V3, P85; de Nys R, 1998, MAR ECOL PROG SER, V162, P79, DOI 10.3354/meps162079; de Nys R, 1999, RECENT ADV MARINE BI, V3, P223; deNys R, 1996, BIOFOULING, V10, P213, DOI 10.1080/08927019609386281; DENYS R, 1993, TETRAHEDRON, V49, P11213, DOI 10.1016/S0040-4020(01)81808-1; DENYS R, 1995, BIOFOULING, V8, P259, DOI 10.1080/08927019509378279; deNys R, 1996, MAR ECOL PROG SER, V130, P135, DOI 10.3354/meps130135; DIXON J, 1981, J PHYCOL, V17, P341, DOI 10.1111/j.1529-8817.1981.tb03120.x; Dworjanyn SA, 1999, MAR BIOL, V133, P727, DOI 10.1007/s002270050514; DWORJANYN SA, 2001, THESIS U NEW S WALES; Hay ME, 1996, J EXP MAR BIOL ECOL, V200, P103, DOI 10.1016/S0022-0981(96)02659-7; HAY ME, 1987, ECOLOGY, V68, P1581, DOI 10.2307/1939850; Henrikson AA, 1995, J EXP MAR BIOL ECOL, V194, P157, DOI 10.1016/0022-0981(95)00088-7; JENNINGS JG, 1994, MAR BIOL, V121, P349, DOI 10.1007/BF00346744; Jennings JG, 1997, OECOLOGIA, V109, P461, DOI 10.1007/s004420050106; JOHNSON CR, 1986, J EXP MAR BIOL ECOL, V96, P127, DOI 10.1016/0022-0981(86)90238-8; Keats DW, 1997, J EXP MAR BIOL ECOL, V213, P281, DOI 10.1016/S0022-0981(96)02771-2; King R.J., 1989, P LINN SOC N S W, V110, P369; Kubanek J, 2002, OECOLOGIA, V131, P125, DOI 10.1007/s00442-001-0853-9; Littler MM, 1999, ECOLOGY, V80, P1736; Maximilien R, 1998, AQUAT MICROB ECOL, V15, P233, DOI 10.3354/ame015233; McKey D, 1979, HERBIVORES THEIR INT, P55; MILLER WT, 1990, IEEE T ROBOTIC AUTOM, V6, P1, DOI 10.1109/70.88112; Nylund GM, 2003, MAR BIOL, V143, P875, DOI 10.1007/s00227-003-1093-9; SCHMITT TM, 1995, ECOLOGY, V76, P107, DOI 10.2307/1940635; Underwood AJ., 1997, EXPT ECOLOGY THEIR L; WAHL M, 1995, OECOLOGIA, V102, P329, DOI 10.1007/BF00329800; WALKER FT, 1948, NATURE, V162, P31; Womersley H. B. S., 1984, MARINE BENTHIC FLORA; Womersley H.B.S., 1996, MARINE BENTHIC FL 3B; Womersley H.B.S., 1987, MARINE BENTHIC FLO 2; Womersley H.B.S., 1994, MARINE BENTHIC FL 3A; Wright JT, 2000, MAR ECOL PROG SER, V207, P227, DOI 10.3354/meps207227	38	69	73	0	60	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0171-8630			MAR ECOL PROG SER	Mar. Ecol.-Prog. Ser.		2006	318						153	163		10.3354/meps318153			11	Ecology; Marine & Freshwater Biology; Oceanography	Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography	082ER	WOS:000240370000013		Bronze, Green Accepted			2021-04-07	
J	Roeder, V; Collen, J; Rousvoal, S; Corre, E; Leblanc, C; Boyen, C				Roeder, V; Collen, J; Rousvoal, S; Corre, E; Leblanc, C; Boyen, C			Identification of stress gene transcripts in Laminaria digitata (Phaeophyceae) protoplast cultures by expressed sequence tag analysis	JOURNAL OF PHYCOLOGY			English	Article						defense; expressed sequences tags; glutathione-S-transferase; Laminaria digitata; mannuronan-C5-epimerase; protoplast; stress; vanadium-dependent bromoperoxidase	GLUTATHIONE S-TRANSFERASES; OXIDATIVE BURST; BROWN-ALGAE; THIOREDOXIN; BROMOPEROXIDASE; HALOPEROXIDASE; SPOROPHYTES; METABOLISM; PROTEINS; ENZYMES	To characterize stress and defense-induced genes in the brown alga Laminaria digitata (Hudson) J.V. Lamouroux, 1985 expressed sequence tags (ESTs) were generated from L. digitata protoplasts. Comparison of the ESTs with public databases allowed putative functions to be assigned to 45% of the sequences. Comparison with ESTs from L. digitata sporophytes showed that protoplasts expressed more stress genes than intact thalli. Several transcripts in the stress gene class coded for proteins involved in cell protection against oxygen radicals, including thioredoxins (six ESTs), thioredoxin peroxidases (two ESTs), and glutathione-S-transferase (GST) (41 ESTs). The GSTs appear to be part of the sigma class, making them the first GST sigma identified in a photosynthetic organism. Other stress genes included a new type of vanadium-dependent bromoperoxidases (vBPO) showing 71% similarity with vBPOs previously identified in the sporophytic-thalli phase of L. digitata. The ESTs coding for 22 different mannuronan-C5-epimerases were identified among the cell wall biosynthesis genes, and several ESTs showed similarity with the genome of the Ectocarpus siliculosus virus.	CNRS, F-29682 Roscoff, France; Univ Paris 06, Biol Stn, F-29682 Roscoff, France	Boyen, C (corresponding author), CNRS, BP 74, F-29682 Roscoff, France.	boyen@sb-roscoff.fr	corre, erwan/O-4669-2019	corre, erwan/0000-0001-6354-2278			Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Armstrong RN, 1997, CHEM RES TOXICOL, V10, P2, DOI 10.1021/tx960072x; Arner ESJ, 2000, EUR J BIOCHEM, V267, P6102, DOI 10.1046/j.1432-1327.2000.01701.x; Audic S, 1997, GENOME RES, V7, P986, DOI 10.1101/gr.7.10.986; BAKER CJ, 1995, ANNU REV PHYTOPATHOL, V33, P299, DOI 10.1146/annurev.py.33.090195.001503; Baldauf SL, 2003, SCIENCE, V300, P1703, DOI 10.1126/science.1085544; Benet H, 1997, PROTOPLASMA, V199, P39, DOI 10.1007/BF02539804; BENET H, 1994, J EXP BOT, V45, P211, DOI 10.1093/jxb/45.2.211; Berglin M, 2004, BIOMACROMOLECULES, V5, P2376, DOI 10.1021/bm0496864; BUTLER A, 1990, J PHYCOL, V26, P589, DOI 10.1111/j.0022-3646.1990.00589.x; BUTLER DM, 1989, J EXP BOT, V40, P1237, DOI 10.1093/jxb/40.11.1237; Colin C, 2005, J BIOL INORG CHEM, V10, P156, DOI 10.1007/s00775-005-0626-8; Colin C, 2003, J BIOL CHEM, V278, P23545, DOI 10.1074/jbc.M300247200; COLLEN J, 2006, IN PRESS J PHYCOL; Crepineau F, 2000, PLANT MOL BIOL, V43, P503, DOI 10.1023/A:1006489920808; De Martino A, 2000, EUR J BIOCHEM, V267, P5540, DOI 10.1046/j.1432-1327.2000.01616.x; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Edwards R, 2000, TRENDS PLANT SCI, V5, P193, DOI 10.1016/S1360-1385(00)01601-0; Ewing B, 1998, GENOME RES, V8, P175, DOI 10.1101/gr.8.3.175; Gurbuxani S, 2001, ONCOGENE, V20, P7478, DOI 10.1038/sj.onc.1204948; Hayes JD, 1999, FREE RADICAL RES, V31, P273, DOI 10.1080/10715769900300851; JORDAN P, 1991, J PLANT PHYSIOL, V137, P520, DOI 10.1016/S0176-1617(11)80692-9; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; KRENN BE, 1989, J BIOL CHEM, V264, P19287; Kruger NJ, 2003, CURR OPIN PLANT BIOL, V6, P236, DOI 10.1016/S1369-5266(03)00039-6; Kupper FC, 1998, PLANTA, V207, P163, DOI 10.1007/s004250050469; Kupper FC, 2002, J CHEM ECOL, V28, P2057, DOI 10.1023/A:1020706129624; Kupper FC, 2001, PLANT PHYSIOL, V125, P278, DOI 10.1104/pp.125.1.278; Kwak JM, 1997, PLANTA, V202, P9, DOI 10.1007/s004250050097; LEE GJ, 1995, J BIOL CHEM, V270, P10432, DOI 10.1074/jbc.270.18.10432; LEGALL Y, 1993, PROTOPLASMA, V173, P123; Lewis RJ, 1996, PHYCOLOGIA, V35, P19, DOI 10.2216/i0031-8884-35-1-19.1; MADGWICK J, 1973, ACTA CHEM SCAND, V27, P3592, DOI 10.3891/acta.chem.scand.27-3592; Maheswari U, 2005, NUCLEIC ACIDS RES, V33, pD344, DOI 10.1093/nar/gki121; Manley SL, 2002, BIOGEOCHEMISTRY, V60, P163, DOI 10.1023/A:1019859922489; Marrs KA, 1996, ANNU REV PLANT PHYS, V47, P127, DOI 10.1146/annurev.arplant.47.1.127; Mittler R, 2002, TRENDS PLANT SCI, V7, P405, DOI 10.1016/S1360-1385(02)02312-9; Moulin P, 1999, J PHYCOL, V35, P1237, DOI 10.1046/j.1529-8817.1999.3561237.x; MULLER EGD, 1991, J BIOL CHEM, V266, P9194; Nollen EAA, 2002, J CELL SCI, V115, P2809; Nyvall P, 2003, PLANT PHYSIOL, V133, P726, DOI 10.1104/pp.103.025981; Ohlrogge J, 2000, CURR OPIN PLANT BIOL, V3, P224, DOI 10.1016/S1369-5266(00)00068-6; Papadakis AK, 2001, PLANT PHYSIOL, V126, P434, DOI 10.1104/pp.126.1.434; Pertea G, 2003, BIOINFORMATICS, V19, P651, DOI 10.1093/bioinformatics/btg034; Potin P, 2002, CURR OPIN PLANT BIOL, V5, P308, DOI 10.1016/S1369-5266(02)00273-X; Ritz D, 2000, J BIOL CHEM, V275, P2505, DOI 10.1074/jbc.275.4.2505; SAENKO GN, 1978, MAR BIOL, V47, P243, DOI 10.1007/BF00541002; Scala S, 2002, PLANT PHYSIOL, V129, P993, DOI 10.1104/pp.010713; SHEFFIELD WP, 1990, J BIOL CHEM, V265, P11069; Weyand M, 1999, J MOL BIOL, V293, P595, DOI 10.1006/jmbi.1999.3179; Wojtaszek P, 1997, BIOCHEM J, V322, P681, DOI 10.1042/bj3220681; Yu HJ, 1999, MOL CELLS, V9, P258	53	71	79	0	29	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	DEC	2005	41	6					1227	1235		10.1111/j.1529-8817.2005.00150.x			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	991QU	WOS:000233829500019					2021-04-07	
J	Fujita, S; Iseki, M; Yoshikawa, S; Makino, Y; Watanabe, M; Motomura, T; Kawai, H; Murakami, A				Fujita, S; Iseki, M; Yoshikawa, S; Makino, Y; Watanabe, M; Motomura, T; Kawai, H; Murakami, A			Identification and characterization of a fluorescent flagellar protein from the brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae): A flavoprotein homologous to Old Yellow Enzyme	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						brown algae; flagella; flavin mononucleotide; flavoprotein; Old Yellow Enzyme; Scytosiphon lomentaria	ECTOCARPUS-SILICULOSUS; PHOTOTACTIC RESPONSES; ARABIDOPSIS-THALIANA; POSTERIOR FLAGELLUM; AUTOFLUORESCENCE; SUBSTANCE; REDUCTASE; SWARMERS; GRACILIS	The posterior flagellum of the zoospore of the brown alga Scytosiphon lomentaria exhibits bright green autofluorescence. To identify the fluorescent flagellar substance(s), we isolated flagella from zoospores and partially purified a flavoprotein by anion-exchange and gel-filtration chromatography. Spectrofluorometric and chromatographic analyses showed that the flavoprotein had an apparent molecular mass of 41 kDa and a non-covalently bound flavin mononucleotide as a chromophore. Based on partial amino acid sequences of the protein, a cDNA of the 41-kDa flavoprotein was cloned and sequenced. The deduced amino acid sequence of the cDNA was homologous to that of the Old Yellow Enzyme family distributed in proteobacteria, yeasts and vascular plants.	Kobe Univ, Res Ctr Inland Seas, Awaji 6562401, Japan; Kobe Univ, Grad Sch Sci & Technol, Kobe, Hyogo 6578501, Japan; Japan Sci & Technol Agcy, PRESTO, Kawaguchi 3320012, Japan; Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan; Hokkaido Univ, Field Sci Ctr No Biosphere, Muroran, Hokkaido 0510003, Japan; Grad Univ Adv Studies, Sch Adv Sci, Dept Photosci, Hayama 2400193, Japan	Murakami, A (corresponding author), Kobe Univ, Res Ctr Inland Seas, Awaji 6562401, Japan.	akiomura@kobe-u.ac.jp			Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [15370039] Funding Source: KAKEN		BESSEY OA, 1949, J BIOL CHEM, V180, P755; Biesgen C, 1999, PLANTA, V208, P155, DOI 10.1007/s004250050545; BOUCK GB, 1971, J CELL BIOL, V50, P362, DOI 10.1083/jcb.50.2.362; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102; COLEMAN AW, 1988, J PHYCOL, V24, P118; French CE, 1995, BIOCHEM J, V312, P671, DOI 10.1042/bj3120671; GELLER A, 1981, J EXP BIOL, V92, P53; Inouye Isao, 1993, P99; Iseki M, 2002, NATURE, V415, P1047, DOI 10.1038/4151047a; Kawai H, 1996, PROTOPLASMA, V191, P172, DOI 10.1007/BF01281815; KAWAI H, 1991, PROTOPLASMA, V161, P17, DOI 10.1007/BF01328893; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KAWAI H, 1988, J PHYCOL, V24, P114; KAWAI H, 1992, BOT MAG TOKYO, V105, P171, DOI 10.1007/BF02489413; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KREIMER G, 1994, INT REV CYTOL, V148, P229, DOI 10.1016/S0074-7696(08)62409-2; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; LIU YG, 1995, PLANT J, V8, P457, DOI 10.1046/j.1365-313X.1995.08030457.x; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; Nagasato C, 2003, J PHYCOL, V39, P1172, DOI 10.1111/j.0022-3646.2003.03-007.x; Pohnert G, 2002, NAT PROD REP, V19, P108, DOI 10.1039/a806888g; SAITO K, 1991, J BIOL CHEM, V266, P20720; Schaller F, 2000, PLANTA, V210, P979, DOI 10.1007/s004250050706; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; VAZ ADN, 1995, BIOCHEMISTRY-US, V34, P4246, DOI 10.1021/bi00013a014; Warburg O, 1933, BIOCHEM Z, V266, P377; Williams RE, 2002, MICROBIOL-SGM, V148, P1607, DOI 10.1099/00221287-148-6-1607; Yamano K, 1996, Z NATURFORSCH C, V51, P155	30	15	15	0	4	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0967-0262	1469-4433		EUR J PHYCOL	Eur. J. Phycol.	MAY	2005	40	2					159	167		10.1080/09670260500063193			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	944LF	WOS:000230429100003		Bronze			2021-04-07	
J	Chen, J; Cassar, SC; Zhang, D; Gopalakrishnan, M				Chen, J; Cassar, SC; Zhang, D; Gopalakrishnan, M			A novel potassium channel encoded by Ectocarpus siliculosus virus	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS			English	Article						potassium channel; Ectocarpus siliculosus virus; Paramecium bursaria chlorella virus; viral life cycle	INFLUENZA-B VIRUS; ION-CHANNEL; CHLORELLA VIRUSES; M(2) PROTEIN; NB PROTEIN; AMANTADINE; MEMBRANE; KCV; REPLICATION; PREDICTION	Kcv, the first identified viral potassium channel encoded by the green algae Paramecium bursaria chlorella virus (PBCV-1), conducted K+ selective currents when expressed in heterologous systems. This K+ channel was proposed to be important for PBCV-1 infection and replication. In the present study, we identified and functionally characterized a novel K+ channel Kesv, encoded by Ectocarpus siliculosus virus that infects filamentous marine brown algae. Kesv encodes a protein of 124 amino acids and is 21.8% identical and 37.1% homologous to Kcv. Membrane topology programs predicted that Kesv consists of three transmembrane domains. When expressed in Xenopus oocytes, Kesv induced largely instantaneous, K+ selective currents that were sensitive to block by Ba2+ and amantadine. Thus, Kesv along with Kcv, constitutes an emerging family of viral potassium channels, which may play important roles in the life cycle of viruses. (C) 2004 Elsevier Inc. All rights reserved.	Abbott Labs, Global Pharmaceut Res & Dev, Neurosci Res, Abbott Pk, IL 60064 USA	Chen, J (corresponding author), Abbott Labs, Global Pharmaceut Res & Dev, Neurosci Res, Abbott Pk, IL 60064 USA.	jun.x.chen@abbott.com	Cassar, Steven/AAW-3201-2020				ASHCROFT FM, 1991, BRIT J PHARMACOL, V104, P579, DOI 10.1111/j.1476-5381.1991.tb12472.x; Betakova T, 1996, J GEN VIROL, V77, P2689, DOI 10.1099/0022-1317-77-11-2689; Chen J, 1999, J BIOL CHEM, V274, P10113, DOI 10.1074/jbc.274.15.10113; Cleaver OB, 1996, DEVELOPMENT, V122, P3549; Cserzo M, 1997, PROTEIN ENG, V10, P673, DOI 10.1093/protein/10.6.673; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Doyle DA, 1998, SCIENCE, V280, P69, DOI 10.1126/science.280.5360.69; DURELL SR, 1992, BIOPHYS J, V62, P238, DOI 10.1016/S0006-3495(92)81809-X; Gazzarrini S, 2004, J BIOL CHEM, V279, P28443, DOI 10.1074/jbc.M401184200; Gazzarrini S, 2003, FEBS LETT, V552, P12, DOI 10.1016/S0014-5793(03)00777-4; Hatta M, 2003, J VIROL, V77, P6050, DOI 10.1128/JVI.77.10.6050-6054.2003; HEGINBOTHAM L, 1994, BIOPHYS J, V66, P1061, DOI 10.1016/S0006-3495(94)80887-2; Hille B., 2001, ION CHANNELS EXCITAB, V3rd edn; Kang M, 2004, P NATL ACAD SCI USA, V101, P5318, DOI 10.1073/pnas.0307824100; Kang M, 2003, FEBS LETT, V552, P2, DOI 10.1016/S0014-5793(03)00775-0; Matsubayashi H, 1997, J PHARMACOL EXP THER, V281, P834; Mehmel M, 2003, FEBS LETT, V552, P7, DOI 10.1016/S0014-5793(03)00776-2; Melen K, 2003, J MOL BIOL, V327, P735, DOI 10.1016/S0022-2836(03)00182-7; Moroni A, 2002, FEBS LETT, V530, P65, DOI 10.1016/S0014-5793(02)03397-5; Mould JA, 2003, DEV CELL, V5, P175, DOI 10.1016/S1534-5807(03)00190-4; Plugge B, 2000, SCIENCE, V287, P1641, DOI 10.1126/science.287.5458.1641; Pringle CR, 1998, ARCH VIROL, V143, P203, DOI 10.1007/s007050050280; SARKAR G, 1990, BIOTECHNIQUES, V8, P404; SCHREMPF H, 1995, EMBO J, V14, P5170, DOI 10.1002/j.1460-2075.1995.tb00201.x; Sonnhammer E L, 1998, Proc Int Conf Intell Syst Mol Biol, V6, P175; STUEHMER W, 1992, METHOD ENZYMOL, V207, P319; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; WANG C, 1993, J VIROL, V67, P5585, DOI 10.1128/JVI.67.9.5585-5594.1993; WANG C, 1994, VIROLOGY, V205, P133, DOI 10.1006/viro.1994.1628; Wang JF, 2004, BIOCHEM BIOPH RES CO, V324, P212, DOI 10.1016/j.bbrc.2004.09.039	30	9	9	0	4	ACADEMIC PRESS INC ELSEVIER SCIENCE	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA	0006-291X			BIOCHEM BIOPH RES CO	Biochem. Biophys. Res. Commun.	JAN 28	2005	326	4					887	893		10.1016/j.bbrc.2004.11.125			7	Biochemistry & Molecular Biology; Biophysics	Biochemistry & Molecular Biology; Biophysics	885YN	WOS:000226193400028	15607752				2021-04-07	
J	Rindi, F; Battelli, C				Rindi, F; Battelli, C			Spatio-temporal variability of intertidal algal assemblages of the Slovenian coast (Gulf of Trieste, northern Adriatic Sea)	BOTANICA MARINA			English	Article						Adriatic Sea; Fucus virsoides; intertidal assemblages; marine algae; spatio-temporal variability	TEMPORAL VARIABILITY; ISTRIAN COAST; FUCACEAN ASSOCIATIONS; SPATIAL VARIABILITY; SHORE ASSEMBLAGES; ROCKY COASTS; MACROALGAE; COMMUNITY; ECOLOGY; SCALES	The algal assemblages of the northern Adriatic Sea have been studied intensively, but few quantitative data on their spatial and temporal dynamics are available. The intertidal assemblages of two coasts on the Gulf of Trieste were studied for a year, and the patterns of spatiotemporal variability were examined at several scales in space and time. Patterns for the whole assemblage and individual species were analysed by multivariate and univariate analyses. The assemblages of the two shores were relatively poor in species and characterised by different compositions. A moderate temporal variation was found, mostly related to differences between the periods winter-spring and summer-autumn. Patterns for individual species were mainly characterised by differences between the two shores (Polysiphonia opaca), interactions among shores and sampling dates (Fucus virsoides, Ulva compressa, Gelidium pusillum, Lophosiphonia obscura) and differences on the spatial scale of tens of metres (Ceramium species in aggregate, Cladophora albida, Ectocarpus siliculosus, Ulva laetevirens). The dissimilarity between the assemblages of the two shores was the emergent pattern, and differences in the texture and composition of the rocky substratum (marl and limestone, respectively) are considered perhaps the main causes of these differences. Future studies in this region should give more attention to this factor.	Natl Univ Ireland Univ Coll Galway, Martin Ryan Inst, Galway, Ireland; Univ Primorska, Fac Educ Koper, SI-6000 Koper, Slovenia	Rindi, F (corresponding author), Natl Univ Ireland Univ Coll Galway, Martin Ryan Inst, Galway, Ireland.	fabio.rindi@nuigalway.ie	Rindi, Fabio/H-7147-2019				Agardh J.G., 1842, ALGAE MARIS MEDITERR, P164; Airoldi L, 2001, MAR BIOL, V138, P1233, DOI 10.1007/s002270100546; Archambault P, 1996, MAR ECOL PROG SER, V136, P111, DOI 10.3354/meps136111; Bacchiocchi F, 2003, ESTUAR COAST SHELF S, V56, P1157, DOI 10.1016/S0272-7714(02)00322-0; BATTELLI C, 1998, ANNALES, V13, P121; Battelli C., 1995, ANNALES HN, V5, P43; Battelli C., 2002, HACQUETIA, V1, P193; Benedetti-Cecchi L, 2000, OECOLOGIA, V123, P406, DOI 10.1007/s004420051028; Benedetti-Cecchi L, 2003, MAR ENVIRON RES, V55, P429, DOI 10.1016/S0141-1136(02)00310-0; Benedetti-Cecchi L, 2003, J EXP MAR BIOL ECOL, V293, P193, DOI 10.1016/S0022-0981(03)00220-X; Benedetti-Cecchi L, 2001, MAR ECOL PROG SER, V215, P79, DOI 10.3354/meps215079; BOUDOURESQUE C-F, 1971, Tethys, V3, P79; CAFFEY HM, 1982, J EXP MAR BIOL ECOL, V63, P119, DOI 10.1016/0022-0981(82)90026-0; Clarke K., 2001, PRIMER V5 USER MANUA; CLARKE KR, 1993, AUST J ECOL, V18, P117, DOI 10.1111/j.1442-9993.1993.tb00438.x; Curiel D, 1998, HYDROBIOLOGIA, V385, P17, DOI 10.1023/A:1003437105147; Curiel D, 2002, BOT MAR, V45, P66, DOI 10.1515/BOT.2002.008; ERCEGOVIC A, 1952, FAUNA FLORA ADRIATIC, V1, P1; Farnsworth EJ, 1996, ECOL MONOGR, V66, P45, DOI 10.2307/2963480; Feldmann J., 1937, REV ALGOLOGIQUE, V10, P1; Fraschetti S, 2001, MAR ECOL PROG SER, V212, P1, DOI 10.3354/meps212001; GIACCONE G, 1971, Informatore Botanico Italiano, V3, P188; Giaccone G, 1967, NOVA THALASSIA, V3, P1; Glasby TM, 1998, MAR FRESHWATER RES, V49, P429, DOI 10.1071/MF98008; GODINI E, 1998, ATTI MUS CIV STOR NA, V41, P197; GUIRY MD, 2004, ALGAEBASE VERSION 2; Hanel R, 2002, PERIOD BIOL, V104, P159; Hauck F., 1885, MEERESALGEN DEUTSCHL; HAY ME, 1994, TRENDS ECOL EVOL, V9, P414, DOI 10.1016/0169-5347(94)90121-X; Komarek J., 1998, CYANOPROKARYOTA, V19, P548; Kutzing F.T., 1843, PHYCOLOGIA GEN, P458; LITTLER MM, 1980, AM NAT, V116, P25, DOI 10.1086/283610; LITTLER MM, 1980, BOT MAR, V23, P161, DOI 10.1515/botm.1980.23.3.161; Menconi M, 1999, J EXP MAR BIOL ECOL, V233, P1, DOI 10.1016/S0022-0981(98)00123-3; MENGE BA, 1993, J EXP MAR BIOL ECOL, V170, P91, DOI 10.1016/0022-0981(93)90131-7; MUNDA I, 1972, BOT MAR, V15, P1, DOI 10.1515/botm.1972.15.1.1; Munda I., 1982, ACTA ADRIAT, V23, P329; Munda I. M., 1980, ACTA ADRIAT, V21, P333; Munda I.M., 1991, ACTA ADRIAT, V32, P683; Munda I. M., 1993, INT J ENVIRON STUD, V43, P185; MUNDA IM, 1993, HYDROBIOLOGIA, V261, P239; MUNDA IM, 1979, NOVA HEDWIGIA, V31, P607; Munda IM, 2000, NOVA HEDWIGIA, V71, P1; OGORELEC B, 1997, ANN SERIES HIST NATU, V11, P187; PAVLOVEC R, 1985, RAZPRAVE 4 RAZRED SL, V26, P219; Piazzi L, 2004, BOT MAR, V47, P105, DOI 10.1515/BOT.2004.010; Piazzi L, 2002, EUR J PHYCOL, V37, P69, DOI 10.1017/S0967026201003432; PIGNATTI S, 1962, MEMORIE I VENETO SCI, V32, P1; RAIMONDI PT, 1988, J EXP MAR BIOL ECOL, V123, P253, DOI 10.1016/0022-0981(88)90046-9; Rindi F, 2004, J EXP MAR BIOL ECOL, V311, P233, DOI 10.1016/j.jembe.2004.05.009; Rindi F, 2000, EUR J PHYCOL, V35, P115, DOI 10.1080/09670260010001735701; SANTELICES B, 1990, OCEANOGR MAR BIOL, V28, P177; Sfriso A, 2003, MAR ENVIRON RES, V56, P617, DOI 10.1016/S0141-1136(03)00046-1; SFRISO A, 1992, ESTUARIES, V15, P517, DOI 10.2307/1352394; Travizi A, 2004, SCI MAR, V68, P145, DOI 10.3989/scimar.2004.68s1145; Underwood A.J., 1997, EXPT ECOLOGY THEIR L, P504; Underwood AJ, 2000, J EXP MAR BIOL ECOL, V250, P97, DOI 10.1016/S0022-0981(00)00181-7; UNDERWOOD AJ, 1981, J EXP MAR BIOL ECOL, V51, P57, DOI 10.1016/0022-0981(81)90154-4; UNDERWOOD AJ, 1994, ECOL APPL, V4, P3, DOI 10.2307/1942110; Underwood AJ, 2003, J EXP MAR BIOL ECOL, V296, P49, DOI 10.1016/S0022-0981(03)00304-6; Underwood AJ, 1996, OECOLOGIA, V107, P212, DOI 10.1007/BF00327905; Vatova A., 1948, NOVA THALASSIA, V1, P43; VUKOVIC A, 1980, BIOL VESTN, V28, P103; Vukovic A., 1982, ACTA ADRIAT, V23, P227; ZANARDINI G, 1841, MEM REAL ACC SCI TOR, V24, P105; ZAVODNIK D, 1977, THALASSIA JUGOSLAVIC, V13, P413; ZAVODNIK D, 1967, THALASSIA JUGOSLAVIC, V3, P105; ZAVODNIK N, 1998, 3 INT WORKSH CAUL TA, P175; ZEI M, 1955, DISS SAZU, V3, P255; ZOREARMANDA M, 1991, ACTA ADRIATICA, V32, P576; ZOREARMANDA M, 1968, STUD REV GEN FISH CO, V34, P1; Zuljevic A, 2003, J MAR BIOL ASSOC UK, V83, P711, DOI 10.1017/S0025315403007689h	72	14	15	1	8	WALTER DE GRUYTER GMBH	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055	1437-4323		BOT MAR	Bot. Marina		2005	48	2					96	105		10.1515/BOT.2005.022			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	932FO	WOS:000229539800003					2021-04-07	
J	Dobretsov, SV; Qian, PY; Wahl, M				Dobretsov, SV; Qian, PY; Wahl, M			Effect of solar ultraviolet radiation on the formation of shallow, early successional biofouling communities in Hong Kong	MARINE ECOLOGY PROGRESS SERIES			English	Article						UV-A; UV-B; UV stress; biofouling communities; South China Sea; community structure; recruitment; larvae	POLYCHAETE HYDROIDS-ELEGANS; LARVAL SETTLEMENT; UV-RADIATION; SPECIES COMPOSITION; DIATOM ASSEMBLAGES; SHORT-TERM; FIELD; INDUCTION; DIVERSITY; RESPONSES	The effect of ambient solar ultraviolet radiation (UVR) on a shallow-water (4 cm) tropical fouling community was assessed during the succession of macrobenthic species on artificial substrates at the Wong Shek fish farm, Hong Kong. The early successional communities developing under 3 radiation treatments (PAR + UV-A + UV-B 280 to 700 nm; PAR + UV-A = 320 to 700 nm, and PAR = 400 to 700 nm) were monitored for 14 wk. A total of 8 species of algae and 8 species of invertebrates colonised the experimental tiles. During the first 8 wk of the experiments, there were no differences among treatments in diversity, percentage of cover of species and the biomass of the colonisers. During the following 6 wk, the communities exposed to UVR had lower species richness than the communities exposed to only PAR had. The species diversity (after 79 and 98 d) of the 3 treatments varied, but the total percentage of species cover and the entire community biomass were not significantly different across the experiment. Juveniles of the polychaete Hydroides elegans and the barnacle Balanus amphitrite, juveniles of the clams Perna viridis and Modiolus comptus, and the algae Enteromorpha sp., Ectocarpus sp. and Cladophora sp. were responsible for the dissimilarity between communities developed under different UVR treatments. The algae constituted a higher percentage of the cover under the full sunlight spectrum, whereas the polychaete, the barnacle and the clams were dominant in the no-UVR treatment. Our outdoor experiment revealed that UVR inhibited the settlement and decreased post-settlement survival of H. elegans. We concluded that UVR affects the composition of early successional, shallow water biofouling communities in tropical waters as well as the settlement and mortality of single species.	Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China; Hong Kong Univ Sci & Technol, Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China; IFM Geomar, Inst Marine Sci, D-24105 Kiel, Germany	Qian, PY (corresponding author), Hong Kong Univ Sci & Technol, Dept Biol, Clear Water Bay, Kowloon, Hong Kong, Peoples R China.	boqianpy@ust.hk	Dobretsov, Sergey/C-9733-2012; Wahl, Martin/D-2038-2016	Dobretsov, Sergey/0000-0002-1769-6388; Wahl, Martin/0000-0001-8703-3857; Qian, Pei-Yuan/0000-0003-4074-9078			BAILEY CA, 1983, APPL ENVIRON MICROB, V46, P44, DOI 10.1128/AEM.46.1.44-49.1983; Baker A, 1995, ULTRAVIOLET RAD CORA, P149; Bingham BL, 2000, J MAR BIOL ASSOC UK, V80, P515, DOI 10.1017/S0025315400002216; Bornman Janet F., 1993, P427; BOTHWELL ML, 1994, SCIENCE, V265, P97, DOI 10.1126/science.265.5168.97; BOTHWELL ML, 1993, J PHYCOL, V29, P24, DOI 10.1111/j.1529-8817.1993.tb00276.x; Bryan PJ, 1998, J EXP MAR BIOL ECOL, V220, P171, DOI 10.1016/S0022-0981(97)00105-6; CHALKER-SCOTT L, 1992, Journal of Shellfish Research, V11, P221; Chiang WL, 2003, ENVIRON SCI TECHNOL, V37, P1089, DOI 10.1021/es0261168; Clarke K., 2001, PRIMER V5 USER MANUA; CLARSON SJ, 1993, TRENDS POLYM SCI, V1, P92; Dobretsov S, 2004, MAR ECOL PROG SER, V271, P133, DOI 10.3354/meps271133; Franklin LA, 1997, EUR J PHYCOL, V32, P207, DOI 10.1080/09670269710001737149; HADFIELD MG, 1994, RECENT DEVELOPMENTS IN BIOFOULING CONTROL, P65; Harder T, 2002, MAR ECOL PROG SER, V229, P105, DOI 10.3354/meps229105; HOAG H, 2003, UV BLINDS BARNACLES; HUANG Z, 1999, ASIAN MARINE BIOL, V16, P77; Huang Z.G., 1982, P 1 INT MAR BIOL WOR, P767; Kuffner IB, 2001, MAR ECOL PROG SER, V217, P251, DOI 10.3354/meps217251; Lau SCK, 2001, MAR BIOL, V138, P321, DOI 10.1007/s002270000453; Lau SCK, 2003, BIOFOULING, V19, P197, DOI 10.1080/0892701031000072163; Lotze HK, 2002, MAR ECOL PROG SER, V243, P57, DOI 10.3354/meps243057; Molis M, 2004, J EXP MAR BIOL ECOL, V302, P51, DOI 10.1016/j.jembe.2003.10.003; Molis M, 2003, MAR ECOL PROG SER, V263, P113, DOI 10.3354/meps263113; PAWLIK JR, 1992, OCEANOGR MAR BIOL, V30, P273; Pechenik JA, 1998, J EXP MAR BIOL ECOL, V226, P51, DOI 10.1016/S0022-0981(97)00237-2; Qiu JW, 1997, MAR ECOL PROG SER, V152, P79, DOI 10.3354/meps152079; Qiu JW, 2003, BIOFOULING, V19, P37, DOI 10.1080/0892701021000060851; Rodriguez CA, 2000, MAR ECOL PROG SER, V193, P305, DOI 10.3354/meps193305; Santas R, 1996, PHOTOCHEM PHOTOBIOL, V64, P435, DOI 10.1111/j.1751-1097.1996.tb03088.x; Santas R, 1998, MAR BIOL, V131, P153, DOI 10.1007/s002270050306; Santas R, 1998, MAR BIOL, V131, P163, DOI 10.1007/s002270050307; Scheltema R.S., 1974, Thalassia Jugosl, V10, P263; Searles PS, 2001, OECOLOGIA, V127, P1, DOI 10.1007/s004420000592; SHAPIRO SS, 1965, BIOMETRIKA, V52, P591, DOI 10.1093/biomet/52.3-4.591; Unabia CRC, 1999, MAR BIOL, V133, P55, DOI 10.1007/s002270050442; UNDERWOOD AJ, 2001, EXPT ECOLOGY THEIR L; WANG BY, 1993, MOL SIMULAT, V10, P1, DOI 10.1080/08927029308022493; Warwick RM, 1995, MAR ECOL PROG SER, V129, P301, DOI 10.3354/meps129301; Wiencke C, 2000, MAR ECOL PROG SER, V197, P217, DOI 10.3354/meps197217; Williamson Craig E., 1996, International Journal of Environmental Studies, V51, P245, DOI 10.1080/00207239608711083; Zar J.H., 1996, BIOSTATISTICAL ANAL, V3rd	42	25	26	0	15	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0171-8630	1616-1599		MAR ECOL PROG SER	Mar. Ecol.-Prog. Ser.		2005	290						55	65		10.3354/meps290055			11	Ecology; Marine & Freshwater Biology; Oceanography	Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography	925UW	WOS:000229079800005		Green Accepted, Bronze			2021-04-07	
J	Peters, AF; Marie, D; Scornet, D; Kloareg, B; Cock, JM				Peters, AF; Marie, D; Scornet, D; Kloareg, B; Cock, JM			Proposal of Ectocarpus siliculosus (Ectocarpales, Phaeophyceae) as a model organism for brown algal genetics and genomics	JOURNAL OF PHYCOLOGY			English	Article						algae; genetics; genomics; marine macroalgae; model species; Phaeophyceae	MYRIOTRICHIA-CLAVAEFORMIS DICTYOSIPHONALES; SCYTOSIPHON-LOMENTARIA SCYTOSIPHONALES; E-FASCICULATUS PHAEOPHYCEAE; LIFE-HISTORY; SEXUAL REPRODUCTION; LAMINARIA-DIGITATA; BLUE-LIGHT; MITOCHONDRIAL GENOME; ARTIFICIAL HYBRIDS; INORGANIC CARBON	The emergence of model organisms that permit the application of a powerful combination of genomic and genetic approaches has been a major factor underlying the advances that have been made in the past decade in dissecting the molecular basis of a wide range of biological processes. However, the phylogenetic distance separating marine macroalgae from these model organisms, which are mostly from the animal, fungi, and higher plant lineages, limits the latters' applicability to problems specific to macroalgal biology. There is therefore a pressing need to develop similar models for the macroalgae. Here we describe a survey of potential model brown algae in which particular attention was paid to characteristics associated with a strong potential for genomic and genetic analysis, such as a small nuclear genome size, sexuality, and a short life cycle. Flow cytometry of nuclei isolated from zoids showed that species from the Ectocarpales possess smaller haploid genomes (127-290 Mbp) than current models among the Laminariales (580-720 Mbp) and Fucales (1095-1271 Mbp). Species of the Ectocarpales may complete their life histories in as little as 6 weeks in laboratory culture and are amenable to genetic analyses. Based on this study, we propose Ectocarpus siliculosus (Dillwyn) Lyngbye as an optimal choice for a general model organism for the molecular genetics of the brown algae.	UPMC, Biol Stn, CNRS Goermar, UMR 7139, F-29682 Roscoff, France; UPMC, Biol Stn, CNRS, UMR 7127, F-29682 Roscoff, France	Peters, AF (corresponding author), UPMC, Biol Stn, CNRS Goermar, UMR 7139, F-29682 Roscoff, France.	apeters@sb-roscoff.fr		Cock, J. Mark/0000-0002-2650-0383; Peters, Akira/0000-0001-5332-199X			Asensi A, 2001, J PHYCOL, V37, P411, DOI 10.1046/j.1529-8817.2001.037003411.x; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; BROWNLEE C, 1986, NATURE, V320, P624, DOI 10.1038/320624a0; Buchholz C, 1999, J APPL PHYCOL, V11, P579, DOI 10.1023/A:1008116828263; Busch S, 2001, EUR J PHYCOL, V36, P61, DOI 10.1080/09670260110001735208; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; CHAPMAN ARO, 1995, PHYCOLOGIA, V34, P1, DOI 10.2216/i0031-8884-34-1-1.1; CLAYTON MN, 1986, BRIT PHYCOL J, V21, P371, DOI 10.1080/00071618600650441; Coelho SM, 2002, PLANT CELL, V14, P2369, DOI 10.1105/tpc.003285; Coyer JA, 2003, MOL ECOL, V12, P1817, DOI 10.1046/j.1365-294X.2003.01850.x; Coyer JA, 2002, EUR J PHYCOL, V37, P173, DOI 10.1017/S0967026202003682; Cronin G, 1996, ECOLOGY, V77, P2287, DOI 10.2307/2265731; DAWIS RH, 2004, NAT REV GENET, V5, P69; Del Campo E, 1997, PHYCOLOGIA, V36, P186, DOI 10.2216/i0031-8884-36-3-186.1; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Dimitriadis Ilias, 2001, Phycological Research, V49, P23; Draisma S. G. A., 2003, OUT PAST COLLECTED R, P87; Fletcher RL, 1987, SEAWEEDS BRIT ISLE 1, VIII; Gall EA, 1996, EUR J PHYCOL, V31, P369, DOI 10.1080/09670269600651601; Gomez I, 2001, EUR J PHYCOL, V36, P391, DOI 10.1080/09670260110001735548; HENRY EC, 1983, PHYCOLOGIA, V22, P387, DOI 10.2216/i0031-8884-22-4-387.1; Hillrichs S, 2001, EUR J PHYCOL, V36, P71, DOI 10.1017/S096702620100302X; HOSHAW RW, 1973, HDB PHYCOLOGICAL MET, P62; Jiang P, 2003, PLANT CELL REP, V21, P1211, DOI 10.1007/s00299-003-0645-2; Katsaros Christos I., 1995, Phycological Research, V43, P43, DOI 10.1111/j.1440-1835.1995.tb00004.x; Kaul S, 2000, NATURE, V408, P796, DOI 10.1038/35048692; Kogame K, 1998, BOT MAR, V41, P339, DOI 10.1515/botm.1998.41.1-6.339; Kogame Kazuhiro, 2001, Phycological Research, V49, P123, DOI 10.1046/j.1440-1835.2001.00232.x; Kogame Kazuhiro, 1996, Phycological Research, V44, P85, DOI 10.1111/j.1440-1835.1996.tb00380.x; Kogame Kazuhiro, 1998, Phycological Research, V46, P39, DOI 10.1111/j.1440-1835.1998.tb00095.x; Kogame K, 1997, PHYCOLOGIA, V36, P389, DOI 10.2216/i0031-8884-36-5-389.1; Kogame K, 1997, PHYCOLOGIA, V36, P337, DOI 10.2216/i0031-8884-36-5-337.1; KUHLENKAMP R, 1994, BOT MAR, V37, P525, DOI 10.1515/botm.1994.37.6.525; LEGALL Y, 1993, PROTOPLASMA, V173, P123; Lewis DK, 1996, ECOL ECON, V16, P35, DOI 10.1016/0921-8009(95)00077-1; LUBCHENCO J, 1980, ECOLOGY, V61, P676, DOI 10.2307/1937433; Maier I, 1997, J PHYCOL, V33, P838, DOI 10.1111/j.0022-3646.1997.00838.x; Maier I, 1997, EUR J PHYCOL, V32, P255; Maier I, 1997, EUR J PHYCOL, V32, P241; Maier I, 1995, PROGR PHYCOL RES, V11, P51; McHugh D.J., 2003, 441 FAO FISH; Mizuta H, 1997, FISHERIES SCI, V63, P553, DOI 10.2331/fishsci.63.553; Muller D. G., 1963, PUBBLICAZIONI STAZIO, V33, P310; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, PROTOPLASMA, V193, P58, DOI 10.1007/BF01276634; MULLER DG, 1976, ARCH MICROBIOL, V109, P89, DOI 10.1007/BF00425117; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1974, BIOCHEM PHYSIOL PFL, V165, P212; MULLER DG, 1981, NATURWISSENSCHAFTEN, V68, P478, DOI 10.1007/BF01047524; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; Muller DG, 2000, BOT MAR, V43, P157, DOI 10.1515/BOT.2000.016; MULLER DG, 1984, PHYCOLOGIA, V23, P87, DOI 10.2216/i0031-8884-23-1-87.1; MULLER DG, 1975, ARCH PROTISTENKD, V117, P297; Nagasato C, 2003, J PHYCOL, V39, P1172, DOI 10.1111/j.0022-3646.2003.03-007.x; Nagasato C, 2000, EUR J PHYCOL, V35, P339, DOI 10.1080/09670260010001735941; Oudot-le Secq MP, 2002, EUR J PHYCOL, V37, P163, DOI 10.1017/S0967026202003542; Oudot-Le Secq MP, 2001, J MOL EVOL, V53, P80, DOI 10.1007/s002390010196; Peters A.F., 1987, PROGR PHYCOLOGICAL R, V5, P223; PETERS AF, 1988, BRIT PHYCOL J, V23, P299, DOI 10.1080/00071618800650331; PETERS AF, 1991, REV CHIL HIST NAT, V64, P261; PETERS AF, 1992, BRIT PHYCOL J, V27, P177, DOI 10.1080/00071619200650181; Peters AF, 2004, EUR J PHYCOL, V39, P235, DOI 10.1080/09670260410001683241; PETERS AF, 1985, HELGOLANDER MEERESUN, V39, P441, DOI 10.1007/BF01987412; PETERS AF, 1991, PHYCOLOGIA, V30, P365, DOI 10.2216/i0031-8884-30-4-365.1; Pohnert G, 2002, NAT PROD REP, V19, P108, DOI 10.1039/a806888g; Reed DC, 2004, J PHYCOL, V40, P275, DOI 10.1046/j.1529-8817.2004.03119.x; Santelices B, 2002, J PHYCOL, V38, P4, DOI 10.1046/j.1529-8817.2002.00193.x; Schmid R, 2001, EUR J PHYCOL, V36, P257, DOI 10.1017/S0967026201003274; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; STACHE B, 1993, THESIS HARTUNG GORRE; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG; VANDENHOEK C, 1968, BLUMEA, V16, P193; Waaland JR, 2004, J PHYCOL, V40, P26, DOI 10.1111/j.0022-3646.2003.03-148.x; Wolf S, 2000, EUR J PHYCOL, V35, P163, DOI 10.1017/S0967026200002663; Worm B, 2001, OECOLOGIA, V128, P281, DOI 10.1007/s004420100648	83	105	111	1	30	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	DEC	2004	40	6					1079	1088		10.1111/j.1529-8817.2004.04058.x			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	878GB	WOS:000225633600009					2021-04-07	
J	Huang, H; Zhong, ZP; Wang, KB; Bai, KZ; Li, LB; Kuang, TY				Huang, H; Zhong, ZP; Wang, KB; Bai, KZ; Li, LB; Kuang, TY			Isolation and characterization of the cytoplasmic membrane from the terrestrial cyanobacterium - Nostoc flagelliforme	ACTA BOTANICA SINICA			English	Article						Nostoc flagelliforme; cytoplasmic membrane; isolation; digitonin	BLUE-GREEN-ALGA; ECTOCARPUS-FASCICULATUS PHAEOPHYCEAE; ANACYSTIS-NIDULANS; SYNECHOCYSTIS PCC6803; LIPIDS; TRANSPORT; MUTANT	Cytoplasmic membrane of Nostoc flagelliforme Born. et Flah. was isolated for the first time with a new method, the unique feature of which is the combined use of French pressure cell and digitonin to disrupt cells. After passed twice through French pressure cell (at 80 MPa), cells in sample (20 mg of dry weight/mL) were disrupted effectively by digitonin (3 mg/mQ, and then the cytoplasmic membrane was isolated by density gradient centrifugation. The membrane contained carotenoids with absorption peaks at 458, 487 and 524 nm and a precursor of chlorophyll a with a minor peak at 673 nm. The fluorescence emission peaks of the membrane were emitted from the precursor of chlorophyll a. More than 30 polypeptides were detected in the membrane, in which the most obvious corresponded to the polypeptides with molecular mass of 80, 28, 19 and 17 kD. The membrane contained four types of glycerolipids: MGDG (62.4%), DGDG (18.9%), SQDG (16.7%) and PG (2.0%). 16:0, 16:1 [9], 18:0, 18:1 [9], 18:2 [9, 12] and 18:3 [9, 12, 15] fatty acids were determined in the membrane, in which 16:1 and 18:3 fatty acids were the main components, representing 32.3% and 34.4% of the total fatty acids respectively. High proportion of 18:3 fatty acid in the cytoplasmic membrane may be an important factor of N. flagelliforme in its remarkable drought-tolerant ability.	Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China	Kuang, TY (corresponding author), Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China.	Kuangty@public.bta.net.cn					Chauhan VS, 2000, CURR MICROBIOL, V41, P321, DOI 10.1007/s002840010143; Gao KS, 1998, J APPL PHYCOL, V10, P37, DOI 10.1023/A:1008014424247; GOMBOS Z, 1992, P NATL ACAD SCI USA, V89, P9959, DOI 10.1073/pnas.89.20.9959; GREEN LS, 1988, J BACTERIOL, V170, P583, DOI 10.1128/jb.170.2.583-587.1988; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; Makewicz A, 1997, PLANT CELL PHYSIOL, V38, P952, DOI 10.1093/oxfordjournals.pcp.a029257; MURATA N, 1981, PLANT CELL PHYSIOL, V22, P855; OGAWA T, 1991, P NATL ACAD SCI USA, V88, P4275, DOI 10.1073/pnas.88.10.4275; OLIE JJ, 1986, APPL ENVIRON MICROB, V52, P706, DOI 10.1128/AEM.52.4.706-710.1986; OMATA T, 1989, P NATL ACAD SCI USA, V86, P6612, DOI 10.1073/pnas.86.17.6612; OMATA T, 1984, ARCH MICROBIOL, V139, P113, DOI 10.1007/BF00401984; OMATA T, 1983, PLANT CELL PHYSIOL, V24, P1101; SCHERER S, 1991, MICROBIAL ECOL, V22, P271, DOI 10.1007/BF02540229; STANIER RY, 1977, ANNU REV MICROBIOL, V31, P225, DOI 10.1146/annurev.mi.31.100177.001301; Tasaka Y, 1996, EMBO J, V15, P6416, DOI 10.1002/j.1460-2075.1996.tb01033.x; Wada H, 1998, LIPIDS PHOTOSYNTHESI, P65; WANG FZ, 1988, RECENT ADV BIOTECHNO, P715; Wang M, 2000, ACTA BOT SIN, V42, P1263; WANG X-L, 1984, Acta Botanica Sinica, V26, P484; Xu YN, 1999, FETT-LIPID, V101, P104; ZHONG Z-P, 1985, Acta Botanica Sinica, V27, P277	21	1	2	0	6	SCIENCE PRESS	BEIJING	16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA	0577-7496	1672-9072		ACTA BOT SIN	Acta Bot. Sin.	OCT	2004	46	10					1186	1191					6	Biochemistry & Molecular Biology; Plant Sciences	Biochemistry & Molecular Biology; Plant Sciences	864GN	WOS:000224622100008					2021-04-07	
J	Gismervik, I				Gismervik, I			Podite carrying ciliates dominate the benthic ciliate community in the kelp forest	AQUATIC MICROBIAL ECOLOGY			English	Article						benthic ciliates; Dysteria spp.; cyrtophorids; kelp forest; epiphytes; abundance	LAMINARIA-HYPERBOREA; FAUNA; CYRTOPHORIDA; COLONIZATION; CILIOPHORA; EPIPHYTE; PROTOZOA	The ciliate fauna on the kelp Laminaria hyperborea and 5 of its epiphytes was sampled in April and August, off the north-west coast of Norway. The lamina of the kelp carried few (old lamina) or no ciliates (new lamina), while podite carrying cyrtophorids of the order Dysteriida prevailed in April on the epiphytes. The highest number of ciliates was found on Ectocarpus sp. (total ciliate number of 20352 +/- 5040 ciliates g(-1) fresh weight, FW), with species of the genus Dysteria (8008 +/- 1928 g(-1) FW) and 1 species of the genus Hartmannula (Hartmannula angustipilosa; 8767 +/- 5202 g(-1) FW) being dominant. In August, the abundance of ciliates was greatly reduced on all epiphytes, although more taxa were observed. Aspidisca spp. (4829 +/- 987 g(-1) FW) dominated total numbers (6080 +/- 1118 ciliates g(-1) FW) on Ectocarpus sp. The infraciliature of 7 species from the family Dysteridae, including Agnathodysteria littoralis, Trochilia sigmoides and Dysteria monostyla, is described. The dysterids and H. angustipilosa carry a flexible podite by which they attach to the surface. The podite may be the key to success in this wave- and current-exposed environment.	Univ Oslo, Sect Marine Biol & Limnol, N-0316 Oslo, Norway	Gismervik, I (corresponding author), Univ Oslo, Sect Marine Biol & Limnol, POB 1069, N-0316 Oslo, Norway.	ingrid.gismervik@bio.uio.no					Armstrong E, 2000, ESTUAR COAST SHELF S, V50, P415, DOI 10.1006/ecss.1999.0577; Christie H, 1998, HYDROBIOLOGIA, V375-76, P49, DOI 10.1023/A:1017021325189; Christie H, 2003, J MAR BIOL ASSOC UK, V83, P687, DOI 10.1017/S0025315403007653h; Deroux G., 1968, Protistologica, V4, P365; DEROUX G, 1965, CR HEBD ACAD SCI, V260, P6689; DEROUX G, 1976, PROTISTOLOGICA, V12, P505; Dragesco J., 1986, Faune Tropicale, V26, P1; Fenchel T, 1987, ECOLOGY PROTOZOA; GISMERVIK I, 1997, MAR POLLUT B, V33, P22; Gong J, 2002, EUR J PROTISTOL, V38, P213, DOI 10.1078/0932-4739-00862; Hillebrand H, 2002, ECOLOGY, V83, P2205, DOI 10.1890/0012-9658(2002)083[2205:COMCBG]2.0.CO;2; KAHL A, 1931, TIERWELT DTL, V21, P243; KAIN JM, 1967, HELGOLAND WISS MEER, V15, P489, DOI 10.1007/BF01618645; Lee JJ, 2000, ILLUSTRATED GUIDE PR, P1; Lippert H, 2001, POLAR BIOL, V24, P512; Mann K.H., 2000, ECOLOGY COASTAL WATE, V2nd ed.; Montagnes DJS, 1993, HDB METHODS AQUATIC, P229; NEWELL RC, 1981, KIELER MEERESFORSCH, V5, P356; Norderhaug KM, 2002, MAR BIOL, V141, P965, DOI 10.1007/s00227-002-0893-7; Sherr EB, 2002, ANTON LEEUW INT J G, V81, P293, DOI 10.1023/A:1020591307260; Sjotun K, 1995, ECOLOGY OF FJORDS AND COASTAL WATERS, P525; Song WB, 1997, ARCH PROTISTENKD, V148, P413, DOI 10.1016/S0003-9365(97)80020-6; STEIN F, 1959, ORGANISMUS INFUSIONS, V1; THANE-FENCHEL A, 1968, Ophelia, V5, P273; TURNER PN, 1995, INVERTEBR BIOL, V114, P202, DOI 10.2307/3226874	25	4	4	1	1	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0948-3055	1616-1564		AQUAT MICROB ECOL	Aquat. Microb. Ecol.	SEP 14	2004	36	3					305	310		10.3354/ame036305			6	Ecology; Marine & Freshwater Biology; Microbiology	Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology	868ET	WOS:000224897600010		Bronze			2021-04-07	
J	Peters, AF; Scornet, D; Muller, DG; Kloareg, B; Cock, JM				Peters, AF; Scornet, D; Muller, DG; Kloareg, B; Cock, JM			Inheritance of organelles in artificial hybrids of the isogamous multicellular chromist alga Ectocarpus siliculosus (Phaeophyceae)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						chloroplast; inheritance; ITS1; marine alga; mitochondrion; rps14-atp8 spacer; Rubisco spacer; Phaeophyceae	MITOCHONDRIAL-DNA; MATERNAL INHERITANCE; FUCUS-SERRATUS; VIRUS GENOME; LIFE-CYCLE; SEQUENCES; TRANSMISSION; CHLOROPLASTS; SEGREGATION; MECHANISMS	Our knowledge about the sexual transmission of mitochondria and plastids (hereafter organelles) in isogamous eukaryotes comes mostly from studies of the yeast Saccharomyces cerepisiae and the green alga Chlamydomonas reinhardtii which are both unicellular species. To investigate organelle inheritance in a multicellular organism with morphological isogamy, we studied the filamentous brown alga Ectocarpus siliculosus, in which each gamete contributes one plastid and at least one mitochondrion to the zygote. We crossed strains whose organelle genotypes were distinguishable by PCR. Hybrids contained only maternal mitochondrial genotypes, indicating uniparental inheritance of this organelle. In contrast, hybrids were chimerical for the plastid genome. In 64 to 75% of cases examined, the two zygotic plastids were partitioned into the two halves of the sporophyte, which developed from the zygote. A smaller number of hybrids deviated from this pattern, suggesting more complex mechanisms such as irregular division, segregation, or exchange of genetic material between plastids. E. siliculosus is the first isogamous heterokont eukaryote in which inheritance of organelles has been determined with molecular markers. The deterministic pseudo-Mendelian mechanism of plastid inheritance in E. siliculosus is unusual among eukaryotes.	CNRS, UMR 7139, Biol Stn, F-29682 Roscoff, France; Univ Konstanz, Fak Biol, D-78457 Constance, Germany	Peters, AF (corresponding author), CNRS, UMR 7139, Biol Stn, F-29682 Roscoff, France.	apeters@sb-roscoff.fr		Cock, J. Mark/0000-0002-2650-0383			Allen JF, 1996, J THEOR BIOL, V180, P135, DOI 10.1006/jtbi.1996.0089; ARMBRUST EV, 1998, MOL BIOL CHLOROPLAST, P93; Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; Birky CW, 2001, ANNU REV GENET, V35, P125, DOI 10.1146/annurev.genet.35.102401.090231; BIRKY CW, 1995, P NATL ACAD SCI USA, V92, P11331, DOI 10.1073/pnas.92.25.11331; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Bromham L, 2003, TRENDS ECOL EVOL, V18, P2, DOI 10.1016/S0169-5347(02)00009-5; Coyer JA, 2002, EUR J PHYCOL, V37, P173, DOI 10.1017/S0967026202003682; Coyer JA, 2002, MOL ECOL NOTES, V2, P35, DOI 10.1046/j.1471-8278 .2001.00138.x; CREASEY A, 1994, MOL BIOCHEM PARASIT, V65, P95, DOI 10.1016/0166-6851(94)90118-X; Draisma S. G. A., 2003, OUT PAST COLLECTED R, P87; Draisma SGA, 2001, J PHYCOL, V37, P586, DOI 10.1046/j.1529-8817.2001.037004586.x; FIELD A, 1976, GENETICS ALGAE, P219; KAUFFMANN H, 1914, Z BOT, V6, P721; Kraan S, 2000, PHYCOLOGIA, V39, P554, DOI 10.2216/i0031-8884-39-6-554.1; Maier I, 1997, EUR J PHYCOL, V32, P241; MARTIN FN, 1989, CURR GENET, V16, P373, DOI 10.1007/BF00340717; McFadden GI, 1996, NATURE, V381, P482, DOI 10.1038/381482a0; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1975, ARCH PROTISTENKD, V117, P297; Nagasato C, 2000, EUR J PHYCOL, V35, P339, DOI 10.1080/09670260010001735941; Nakamura S, 2003, PROTOPLASMA, V221, P205, DOI 10.1007/s00709-002-0053-4; Nunnari J, 1997, MOL BIOL CELL, V8, P1233; Oudot-le Secq MP, 2002, EUR J PHYCOL, V37, P163, DOI 10.1017/S0967026202003542; REMACLE C, 1998, MOL BIOL CHLOROPLAST, P661; Rousseau F, 2001, CR ACAD SCI III-VIE, V324, P305, DOI 10.1016/S0764-4469(01)01306-3; Smith G. M., 1950, FRESHWATER ALGAE US; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; Sutovsky P, 1999, NATURE, V402, P371, DOI 10.1038/46466; Yoon HS, 2002, P NATL ACAD SCI USA, V99, P15507, DOI 10.1073/pnas.242379899	35	51	51	0	18	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0967-0262	1469-4433		EUR J PHYCOL	Eur. J. Phycol.	AUG	2004	39	3					235	242		10.1080/09670260410001683241			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	855OC	WOS:000223982600001		Bronze			2021-04-07	
J	Penchaszadeh, PE; Bigatti, G; Miloslavich, P				Penchaszadeh, PE; Bigatti, G; Miloslavich, P			Feeding of Pseudechinus magellanicus (Philippi, 1857) (Echinoidea : Temnopleuridae) in the SW Atlantic Coast (Argentina)	OPHELIA			English	Article						sea urchin; Argentina; MDS; diet; pedicellaria	COMMUNITY STRUCTURE; SEA-URCHINS	Gut contents of Pseudechinus magellanicus collected from different habitats from Buenos Aires to Ushuaia were analyzed. In mussel beds off the Province of Buenos Aires at 50 in depth, the most important food items were Mytilus edulis platensis spat. In communities without Mytilus, food preferences were barnacles, foraminiferans, polychaetes and algae. In beds of the mytilid Aulacomya atra at a depth of 5-10m, main food items were oyster shell fragments and different algae: Rodophyta (leafy algae), Ectocarpus sp., and other algae. In Ushuaia the sea urchins preyed on ostracods, foraminiferans and algae. Multidimensional scaling ordination analyses showed that the diet of P magellanicus depends mostly on the kind of inhabited substrate. Our data indicate that P magellanicus from Argentina is omnivorous, consuming any available food resource: they capture drift algae with the aboral tube feet, graze on sessile algae, and prey on other animals. The results suggest that the species play an important role in the community structure of the circa-littoral beds of Mytilus edulis platensis, mainly through an intense predation on bivalve spat.	UBA, Fac Ciencias Exactas & Nat, Buenos Aires, DF, Argentina; Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat, RA-1405 Buenos Aires, DF, Argentina; Univ Simon Bolivar, Dept Estudios Ambientales, Caracas 1080, Venezuela	Penchaszadeh, PE (corresponding author), UBA, Fac Ciencias Exactas & Nat, Buenos Aires, DF, Argentina.	penchas@bg.feen.uba.ar; gbigatti@bg.fcen.uba.ar		Miloslavich, Patricia/0000-0001-5409-1401; Penchaszadeh, Pablo E/0000-0002-2043-8814			BERNASCONI I, 1953, ANALES MUSEO HIST  6, V2, P17; Castilla J.C., 1982, INT ECH C TAMP BAY, P257; CLARKE KR, 1993, AUST J ECOL, V18, P117, DOI 10.1111/j.1442-9993.1993.tb00438.x; CONTRERAS S, 1987, MAR ECOL PROG SER, V38, P217, DOI 10.3354/meps038217; de Ridder C., 1982, P57; HARROLD C, 1985, ECOLOGY, V66, P1160, DOI 10.2307/1939168; Harrold C., 1987, Echinoderm Studies, V2, P137; KURTEN M, 2002, ESTRUCTURA COMUNIDAD; Lawrence J.M., 1975, Oceanography mar Biol, V13, P213; McClary D, 1998, INVERTEBR BIOL, V117, P75, DOI 10.2307/3226854; MCCLINTOCK JB, 1982, B MAR SCI, V32, P365; PEARSE V, 1997, LIVING INVERTEBRATES; PENCHASZADEH PE, 1980, CAH BIOL MAR, V21, P169; PENCHASZADEH PE, 1973, CONTRIBUCION I BIOL, V234; PENCHASZADEH PE, 1998, ECHINODERM RES 1998, P525; Penchaszadeh PE, 1973, ECOLOGIA BUENOS AIRE, V1, P45; SHARP DT, 1962, ECOLOGY, V43, P309, DOI 10.2307/1931986; VAZQUEZ JA, 1984, MARINE ECOLOGY PROGR, V19, P55	18	10	11	0	4	OPHELIA PUBLICATIONS	STENSTRUP	KIRKEBY SAND 19, DK-5771 STENSTRUP, DENMARK	0078-5326			OPHELIA	Ophelia	AUG	2004	58	2					91	99		10.1080/00785326.2004.10410216			9	Marine & Freshwater Biology	Marine & Freshwater Biology	851YG	WOS:000223721800003					2021-04-07	
J	Dobretsov, S; Dahms, HU; Qian, PY				Dobretsov, S; Dahms, HU; Qian, PY			Antilarval and antimicrobial activity of waterborne metabolites of the sponge Callyspongia (Euplacella) pulvinata: evidence of allelopathy	MARINE ECOLOGY PROGRESS SERIES			English	Article						sponge; antifouling defence; assays; recruitment; larvae; bacteria; diatoms; biofouling communities; waterborne metabolites; epibiosis	MUSSEL MYTILUS-EDULIS; HONG-KONG WATERS; MARINE SPONGE; BALANUS-AMPHITRITE; MICROBIAL COMMUNITY; APLYSINA-FISTULARIS; LARVAL SETTLEMENT; CHEMICAL ECOLOGY; HYDROIDS-ELEGANS; REEF SPONGE	Antilarval, antidiatom and antibacterial activity of sponge metabolites has been well documented in previous laboratory experiments. In this investigation we attempted to link the results of our earlier laboratory experiments of antifouling activity of the sponge Callyspongia (Euplacella) pulvinata (Porifera: Demospongiae, Haliclonidae) to those measured in the field. Our laboratory experiments showed that conditioned seawater (CSW) of the sponge strongly inhibited the growth of the benthic diatom Nitzschia paleaceae and the settlement of the tubeworm Hydroides elegans, but had no effect on the growth of 9 bacterial strains isolated from natural biofilms. When diluted 5 times, the CSW still exhibited antilarval and antidiatom activities. In field experiments, sponge specimens were placed within 13 and 50 cm of Petri dishes that were serving as attachment substrates for microand macrofouling organisms. Synthetic sponge-like material was used to mimic sponges for the controls. The results showed that after 7 d, diatom attachment on dishes placed in close vicinity to the sponge was inhibited. Bacterial densities on the experimental dishes did not differ significantly (p > 0.05) from those in the control dishes. Microfouling communities, which developed on both experimental and control dishes in the field, had similar effects on the settlement of H. elegans under laboratory conditions. After 28 d, an early community had been established, consisting of 5 species of green algae, 2 species of brown algae, 2 species of red algae and 5 species of invertebrates. We found strong negative effects of the presence of sponges and the position of the plate on the total percentage of cover, Shannon-Wiener diversity, as well as on the species richness of macrofouling communities. In the experimental dishes, the percentage of coverage of both macroalgae (Ulva sp., Ectocarpus sp., Enteromorpha sp., unidentified brown algae) and invertebrates (Obelia sp., H. elegans) decreased. ANOSIM (analysis of similarity) and a SIMPER (similarity percentage) analysis demonstrated that the composition of macrofoulers on the dishes were positively affected both by the presence of a sponge and with decreasing distance from it. Our results suggest that waterborne compounds of C. pulvinata inhibit settlement of micro- and macrofoulers not only on its own surface but also on non-living surfaces nearby.	Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China; Hong Kong Univ Sci & Technol, Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China	Qian, PY (corresponding author), Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China.	boqianpy@ust.hk	Dobretsov, Sergey/C-9733-2012	Dobretsov, Sergey/0000-0002-1769-6388; Qian, Pei-Yuan/0000-0003-4074-9078			Acar J.F., 1980, ANTIBIOTICS LAB MED, P24; Armstrong E, 2001, HYDROBIOLOGIA, V461, P37, DOI 10.1023/A:1012756913566; AUSTIN B, 2001, RECENT ADV MARINE BI, V6, P1; BAKUS GJ, 1986, J CHEM ECOL, V12, P951, DOI 10.1007/BF01638991; BECERRO MA, 1994, J EXP MAR BIOL ECOL, V179, P195, DOI 10.1016/0022-0981(94)90114-7; Becerro MA, 1997, HYDROBIOLOGIA, V355, P77, DOI 10.1023/A:1003019221354; Bergquist PR., 1978, SPONGES; Bily Michal, 2002, Archiv fuer Hydrobiologie Supplement, V142, P145; BINGHAM BL, 1991, MAR BIOL, V109, P19, DOI 10.1007/BF01320227; Blunt JW, 2003, NAT PROD REP, V20, P1, DOI 10.1039/b207130b; Bryan PJ, 1998, J EXP MAR BIOL ECOL, V220, P171, DOI 10.1016/S0022-0981(97)00105-6; Burja AM, 2001, HYDROBIOLOGIA, V461, P41, DOI 10.1023/A:1012713130404; Clare AS, 2000, BIOFOULING, V15, P57, DOI 10.1080/08927010009386298; Clarke K., 2001, PRIMER V5 USER MANUA; Davis A.R., 1989, Bioorganic Marine Chemistry, V3, P85; Davis AR, 1998, BIOFOULING, V12, P305, DOI 10.1080/08927019809378362; de Nys R, 1999, RECENT ADV MARINE BI, V3, P223; Dobretsov S, 2001, HYDROBIOLOGIA, V445, P27, DOI 10.1023/A:1017502126707; Dobretsov SV, 2002, BIOFOULING, V18, P217, DOI 10.1080/08927010290013026; Dobretsov SV, 2001, MAR ECOL PROG SER, V218, P179, DOI 10.3354/meps218179; Dobretsov SV, 1999, BIOFOULING, V14, P153, DOI 10.1080/08927019909378406; Friedrich AB, 2001, FEMS MICROBIOL ECOL, V38, P105, DOI 10.1111/j.1574-6941.2001.tb00888.x; Glasby TM, 2001, MAR ECOL PROG SER, V214, P127, DOI 10.3354/meps214127; Harder T, 2002, J CHEM ECOL, V28, P2029, DOI 10.1023/A:1020702028715; Hentschel U, 2001, FEMS MICROBIOL ECOL, V35, P305, DOI 10.1111/j.1574-6941.2001.tb00816.x; Hentschel U, 2002, APPL ENVIRON MICROB, V68, P4431, DOI 10.1128/AEM.68.9.4431-4440.2002; Holler U, 2000, MYCOL RES, V104, P1354, DOI 10.1017/S0953756200003117; HOLMSTROM C, 1992, APPL ENVIRON MICROB, V58, P2111; HUANG Z, 1999, ASIAN MARINE BIOL, V16, P77; Huang Z.G., 1982, P 1 INT MAR BIOL WOR, P767; James SG, 1996, APPL ENVIRON MICROB, V62, P2783, DOI 10.1128/AEM.62.8.2783-2788.1996; Jennings JG, 1997, OECOLOGIA, V109, P461, DOI 10.1007/s004420050106; Jin D.X., 1985, MARINE BENTHIC DIATO, V1; Kelman D, 2001, AQUAT MICROB ECOL, V24, P9, DOI 10.3354/ame024009; KIRCHMAN D, 1981, J EXP MAR BIOL ECOL, V56, P153, DOI 10.1016/0022-0981(81)90186-6; KOBAYASHI M, 1998, SPONGE SCI MULTIDISC, P377; Lau SCK, 2003, J EXP MAR BIOL ECOL, V282, P43, DOI 10.1016/S0022-0981(02)00445-8; Lau SCK, 2002, MAR ECOL PROG SER, V226, P301, DOI 10.3354/meps226301; Lau SCK, 1997, MAR ECOL PROG SER, V159, P219, DOI 10.3354/meps159219; Lee OO, 2003, BIOFOULING, V19, P171, DOI 10.1080/0892701021000055000; MAKI JS, 1988, MAR BIOL, V97, P199, DOI 10.1007/BF00391303; Margot H, 2002, MAR BIOL, V140, P739, DOI 10.1007/s00227-001-0740-2; Marin A, 1998, MAR BIOL, V131, P639, DOI 10.1007/s002270050356; Maximilien R, 1998, AQUAT MICROB ECOL, V15, P233, DOI 10.3354/ame015233; Moeseneder MM, 2001, LIMNOL OCEANOGR, V46, P95, DOI 10.4319/lo.2001.46.1.0095; MORSE DE, 1991, BIOL BULL, V181, P104, DOI 10.2307/1542493; Newbold RW, 1999, AQUAT MICROB ECOL, V19, P279, DOI 10.3354/ame019279; PAWLIK JR, 1992, OCEANOGR MAR BIOL, V30, P273; Pechenik JA, 1998, J EXP MAR BIOL ECOL, V226, P51, DOI 10.1016/S0022-0981(97)00237-2; Qiu JW, 2003, BIOFOULING, V19, P37, DOI 10.1080/0892701021000060851; Relini G, 1998, INT BIODETER BIODEGR, V41, P41, DOI 10.1016/S0964-8305(98)80007-3; Richelle-Maurer E, 2003, J BIOTECHNOL, V100, P169, DOI 10.1016/S0168-1656(02)00251-1; RUETZLER K, 1985, NEW PERSPECTIVES SPO, P455; Sepcic K, 1997, J NAT PROD, V60, P991, DOI 10.1021/np970292q; Sera Y, 1999, J NAT PROD, V62, P395, DOI 10.1021/np980440s; SHAPIRO SS, 1965, BIOMETRIKA, V52, P591, DOI 10.1093/biomet/52.3-4.591; Steinberg PD, 2001, CRC MAR SCI, P355; Steinberg PD, 2002, J CHEM ECOL, V28, P1935, DOI 10.1023/A:1020789625989; Targett N.M., 1988, P609; Thacker RW, 2003, MAR BIOL, V142, P643, DOI 10.1007/s00227-002-0971-x; Thakur NL, 2000, J CHEM ECOL, V26, P57, DOI 10.1023/A:1005485310488; THOMPSON JE, 1985, MAR BIOL, V88, P23, DOI 10.1007/BF00393039; THOMPSON JE, 1983, ACTA ZOOL-STOCKHOLM, V64, P199, DOI 10.1111/j.1463-6395.1983.tb00801.x; THOMPSON JE, 1985, MAR BIOL, V88, P11, DOI 10.1007/BF00393038; Thoms C, 2003, MAR BIOL, V142, P685, DOI 10.1007/s00227-002-1000-9; Tomono Yasuhiko, 1998, P413; Tsukamoto S, 1997, BIOFOULING, V11, P283, DOI 10.1080/08927019709378337; UNSON MD, 1994, MAR BIOL, V119, P1, DOI 10.1007/BF00350100; Wahl M, 1999, MAR ECOL PROG SER, V187, P59, DOI 10.3354/meps187059; Wahl M, 2001, J EXP MAR BIOL ECOL, V258, P101, DOI 10.1016/S0022-0981(00)00348-8; Wahl Martin, 1997, P31; WALKER RP, 1985, MAR BIOL, V88, P27, DOI 10.1007/BF00393040; Warwick RM, 1995, MAR ECOL PROG SER, V129, P301, DOI 10.3354/meps129301; Wieczorek SK, 1998, BIOFOULING, V12, P81, DOI 10.1080/08927019809378348; Yan Tao, 1999, Chinese Journal of Oceanology and Limnology, V17, P233; Zar J.H., 1996, BIOSTATISTICAL ANAL, V3rd	76	27	29	0	19	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0171-8630	1616-1599		MAR ECOL PROG SER	Mar. Ecol.-Prog. Ser.		2004	271						133	146		10.3354/meps271133			14	Ecology; Marine & Freshwater Biology; Oceanography	Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography	827DT	WOS:000221879800011		Bronze			2021-04-07	
J	Kupper, H; Setlik, I; Hlasek, M				Kupper, H; Setlik, I; Hlasek, M			A versatile chamber for simultaneous measurements of oxygen exchange and fluorescence in filamentous and thallous algae as well as higher plants	PHOTOSYNTHETICA			English	Article						Antithamnion; chlorophyll fluorescence induction; Ectocarpus; gas exchange; photosynthetic oxygen release; photosynthesis; submerged aquatic plants	CHLOROPHYLL FLUORESCENCE; SINGLE CELLS; PHOTOSYNTHESIS; MICROELECTRODE; FLUXES	A new chamber was developed for a simultaneous measurement of fluorescence kinetics and oxygen exchange in filamentous and thallous algae as well as in small leaves of water plants. Algal filaments or thalli are kept by a stainless grid close to the bottom window of the chamber in the sample compartment. The grid separates the object from the electrode compartment with the oxygen electrode at the top. This compartment accommodates, in addition, a magnetic stirrer that provides efficient circulation of the medium between the sample and the electrode. This magnetic bar spins on a fixed axis and is driven by an electronically commutated magnetic field produced by four coils which are arranged around the chamber. This design yields a very favourable signal to noise ratio in the oxygen electrode records. Consequently, measurements can be performed even of algae with very low photosynthetic rates such as marine low-light red algae or algae under severe stress. For irradiation of the samples and for fluorescence measurements a fibre optic light guide is used facing the window of the chamber. The four branches of a commercially available light guide serve the following purposes: collection of sample fluorescence and supply of measuring, actinic, and saturating light, respectively.	Univ S Bohemia, Inst Phys Biol, Nove Hrady 37333, Czech Republic; Univ Konstanz, Math Naturwissensch Sekt, Fachbereich Biol, D-78457 Constance, Germany; Acad Sci Czech Republ, Dept Autotroph Microorganisms, Inst Microbiol, CZ-37981 Trebon, Czech Republic; Ing Milan Hlasek Ltron, CZ-37901 Trebon, Czech Republic	Kupper, H (corresponding author), Univ S Bohemia, Inst Phys Biol, Nove Hrady 37333, Czech Republic.	Hendrik.Kuepper@uni-konstanz.de	Kupper, Hendrik/J-5152-2012	Kupper, Hendrik/0000-0003-0712-7023			Campbell D, 1998, MICROBIOL MOL BIOL R, V62, P667, DOI 10.1128/MMBR.62.3.667-683.1998; CLARK LC, 1956, T AM SOC ART INT ORG, V2, P41; Dring MJ, 1996, MAR BIOL, V126, P183, DOI 10.1007/BF00347443; Kupper H, 2002, J PHYCOL, V38, P429, DOI 10.1046/j.1529-8817.2002.01148.x; Land SC, 1999, J EXP BIOL, V202, P211; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; Mancuso S, 2000, PLANTA, V211, P384, DOI 10.1007/s004250000296; Masojidek J, 2000, J APPL PHYCOL, V12, P417, DOI 10.1023/A:1008165900780; Pearson G, 2000, MAR ECOL PROG SER, V202, P67, DOI 10.3354/meps202067; Porterfield DM, 2000, PROTOPLASMA, V212, P80, DOI 10.1007/BF01279349; Yasukawa T, 2000, ELECTROANAL, V12, P653, DOI 10.1002/1521-4109(200005)12:9<653::AID-ELAN653>3.0.CO;2-S	11	5	5	1	8	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0300-3604			PHOTOSYNTHETICA	Photosynthetica		2004	42	4					579	583		10.1007/S11099-005-0016-z			5	Plant Sciences	Plant Sciences	898WR	WOS:000227107400014					2021-04-07	
J	Delaroque, N; Boland, W; Muller, DG; Knippers, R				Delaroque, N; Boland, W; Muller, DG; Knippers, R			Comparisons of two large phaeoviral genomes and evolutionary implications	JOURNAL OF MOLECULAR EVOLUTION			English	Article						viral evolution; phaeovirus; phycodnavirus; Ectocarpus siliculosus virus (EsV)	BROWN ALGAL VIRUS; ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; REPLICATION FACTOR-C; DNA-REPLICATION; PROTELOMERASE; SEQUENCE; ORIGIN; HOST; BACTERIOPHAGES; FAMILIES	The evolution of viral genomes has recently attracted considerable attention. We compare the sequences of two large viral genomes, EsV-1 and FirrV-1, belonging to the family of phaeoviruses which infect different species of marine brown algae. Although their genomes differ substantially in size, these viruses share similar morphologies and similar latent infection cycles. In fact, sequence comparisons show that the viruses have more than 60% of their genes in common. However, the order of genes is completely different in the two genomes, suggesting that extensive recombinational events in addition to several large deletions had occurred during the separate evolutionary routes from a common ancestor. We investigated genes encoding components of signal transduction pathways and genes encoding replicative functions in more detail. We found that the two genomes possess different, although overlapping, sets of genes in both classes, suggesting that different genes from each class were lost, perhaps randomly, after the separate evolution from an ancestral genome. Random loss would also account for the fact that more than one-third of the genes in one viral genome has no counterparts in the other genome. We speculate that the ancestral genome belonged to a cellular organism that had once invaded a primordial brown algal host.	Max Planck Inst Chem Okol, D-07745 Jena, Germany; Univ Konstanz, Dept Biol, D-78457 Constance, Germany	Delaroque, N (corresponding author), Max Planck Inst Chem Okol, Beutenberg Campus,Hans Knoll Str 8, D-07745 Jena, Germany.		Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Bateman A, 2002, NUCLEIC ACIDS RES, V30, P276, DOI 10.1093/nar/30.1.276; Bell PJL, 2001, J MOL EVOL, V53, P251; BLASCO R, 1995, MOL BASIS VIRUS EVOL, P255; Bohlke K, 2002, EXTREMOPHILES, V6, P1, DOI 10.1007/s007920100222; Botstein D, 1980, Ann N Y Acad Sci, V354, P484, DOI 10.1111/j.1749-6632.1980.tb27987.x; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; BRENDEL V, 1992, P NATL ACAD SCI USA, V89, P2002, DOI 10.1073/pnas.89.6.2002; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Delaroque N, 2000, VIROLOGY, V273, P383, DOI 10.1006/viro.2000.0383; Deneke J, 2002, J BIOL CHEM, V277, P10410, DOI 10.1074/jbc.M111769200; Deneke J, 2000, P NATL ACAD SCI USA, V97, P7721, DOI 10.1073/pnas.97.14.7721; Falquet L, 2002, NUCLEIC ACIDS RES, V30, P235, DOI 10.1093/nar/30.1.235; Gibbs A., 1995, MOL BASIS VIRUS EVOL, DOI New York, NY, USA; GORBALENYA AE, 1990, FEBS LETT, V262, P145, DOI 10.1016/0014-5793(90)80175-I; Gray MW, 1999, SCIENCE, V283, P1476, DOI 10.1126/science.283.5407.1476; Hendrix RW, 2002, THEOR POPUL BIOL, V61, P471, DOI 10.1006/tpbi.2002.1590; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; Hubscher U, 2002, ANNU REV BIOCHEM, V71, P133, DOI 10.1146/annurev.biochem.71.090501.150041; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Iyer LM, 2001, J VIROL, V75, P11720, DOI 10.1128/JVI.75.23.11720-11734.2001; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; LAMBERT PF, 1991, J VIROL, V65, P3417, DOI 10.1128/JVI.65.7.3417-3420.1991; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; Lawrence JG, 2002, J BACTERIOL, V184, P4891, DOI 10.1128/JB.184.17.4891-4905.2002; LEE AM, 1995, VIROLOGY, V212, P474, DOI 10.1006/viro.1995.1505; Lee AM, 1998, J PHYCOL, V34, P608, DOI 10.1046/j.1529-8817.1998.340608.x; Lee AM, 1998, VIROLOGY, V248, P35, DOI 10.1006/viro.1998.9245; Letunic I, 2002, NUCLEIC ACIDS RES, V30, P242, DOI 10.1093/nar/30.1.242; LURIA SE, 1968, GEN VIROLOGY; Milner D. G., 2001, SPRINGER INDEX VIRUS, P732; Mossi R, 1998, EUR J BIOCHEM, V254, P209, DOI 10.1046/j.1432-1327.1998.2540209.x; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; Nunes-Duby SE, 1998, NUCLEIC ACIDS RES, V26, P391, DOI 10.1093/nar/26.2.391; Pisani FM, 2000, J MOL BIOL, V301, P61, DOI 10.1006/jmbi.2000.3964; Ravin NV, 2001, J MOL BIOL, V312, P899, DOI 10.1006/jmbi.2001.5019; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; SCHUSTER AM, 1990, VIROLOGY, V176, P515, DOI 10.1016/0042-6822(90)90021-I; Simmons DT, 2000, ADV VIRUS RES, V55, P75, DOI 10.1016/S0065-3527(00)55002-7; Stock AM, 2000, ANNU REV BIOCHEM, V69, P183, DOI 10.1146/annurev.biochem.69.1.183; Strauss JH, 2001, CELL, V105, P5, DOI 10.1016/S0092-8674(01)00291-4; Takemura M, 2001, J MOL EVOL, V52, P419, DOI 10.1007/s002390010171; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; Van Etten JL, 2002, ARCH VIROL, V147, P1479, DOI 10.1007/s00705-002-0822-6; Villarreal LP, 2000, J VIROL, V74, P7079, DOI 10.1128/JVI.74.15.7079-7084.2000; West AH, 2001, TRENDS BIOCHEM SCI, V26, P369, DOI 10.1016/S0968-0004(01)01852-7; Zhang YX, 2002, NATURE, V415, P644, DOI 10.1038/415644a	51	27	28	0	3	SPRINGER-VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010 USA	0022-2844			J MOL EVOL	J. Mol. Evol.	DEC	2003	57	6					613	622		10.1007/s00239-003-2501-y			10	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity	752NK	WOS:000187173900002	14745530				2021-04-07	
J	Jacobsen, S; Luning, K; Goulard, F				Jacobsen, S; Luning, K; Goulard, F			Circadian changes in relative abundance of two photosynthetic transcripts in the marine macroalga Kappaphycus alvarezii (Rhodophyta)	JOURNAL OF PHYCOLOGY			English	Article						circadian clock; marine macroalgae; photosynthesis; phycoerythrin; red algae; ribulose-1,5-bisphoshate carboxylase oxygenase; transcriptional gene expression	RED ALGA; CHLAMYDOMONAS-REINHARDTII; RUBISCO OPERON; ENDOGENOUS FLUCTUATIONS; ECTOCARPUS PHAEOPHYTA; ARABIDOPSIS-THALIANA; RHODELLA-VIOLACEA; GENE-EXPRESSION; FAST RESPONSES; BLUE-LIGHT	Circadian regulation of transcriptional gene expression has been shown for a variety of taxa throughout the eukaryotic kingdom and for prokaryotic cyanobacteria but not previously for macroalgae. We reported earlier a strong circadian rhythm of photosynthetic capacity in the red macroalga Kappaphycus alvarezii (Doty) Doty. To determine whether this rhythm is correlated with circadian regulation of steady-state transcript abundance, we analyzed transcripts of genes whose protein products are involved in photosynthesis. Transcript levels of two plastid-encoded operons, one for phycoerythrin alpha and beta subunits and another for RUBISCO large and small subunits, were analyzed under light: dark cycles (LD) and under constant illumination (LL) for several days. Both transcripts exhibited diurnal regulation under LD conditions with maximum transcript abundance during early daytime to midday. Under LL conditions, circadian oscillations of steady-state mRNA abundance were detected for up to three cycles; thus, the analyzed transcripts exhibited diurnal and circadian regulation. Interestingly, peak abundance of the RUBISCO transcript shifted to subjective night under LL. To our knowledge, this is the first report demonstrating that circadian control of steady-state transcript abundance occurs in a macroalga.	Alfred Wegener Inst Polar & Marine Res, Helmholtz Gemeinschaft, Wattenmeerstn Sylt, D-25992 List Auf Sylt, Germany; UBO, Lab Ecophysiol & Biotechnol Halophytes & Algues M, IUEM, F-29280 Plouzane, France	Jacobsen, S (corresponding author), Alfred Wegener Inst Polar & Marine Res, Helmholtz Gemeinschaft, Wattenmeerstn Sylt, Hafenstr 43, D-25992 List Auf Sylt, Germany.			GOULARD, Fabienne/0000-0003-4812-0872			APT KE, 1993, PLANT MOL BIOL, V21, P27, DOI 10.1007/BF00039615; Ask EI, 2002, AQUACULTURE, V206, P257, DOI 10.1016/S0044-8486(01)00724-4; ASSALI NE, 1991, PLANT MOL BIOL, V17, P853, DOI 10.1007/BF00037066; BERNARD C, 1992, P NATL ACAD SCI USA, V89, P9564, DOI 10.1073/pnas.89.20.9564; BRITZ SJ, 1976, PLANT PHYSIOL, V58, P22, DOI 10.1104/pp.58.1.22; Devlin PF, 2002, J EXP BOT, V53, P1535, DOI 10.1093/jxb/erf024; Doyle MR, 2002, NATURE, V419, P74, DOI 10.1038/nature00954; DRIESSCHE TV, 1966, EXP CELL RES, V42, P18; Dunlap JC, 1999, CELL, V96, P271, DOI 10.1016/S0092-8674(00)80566-8; Fejes E, 1998, BIOL RHYTHMS PHOTOPE, P99; Fujiwara S, 1996, PLANT MOL BIOL, V32, P745, DOI 10.1007/BF00020215; Granbom M, 2001, J PHYCOL, V37, P1020, DOI 10.1046/j.1529-8817.2001.01040.x; Green RM, 2002, DEV CELL, V2, P516, DOI 10.1016/S1534-5807(02)00184-3; GROBBELAAR N, 1986, FEMS MICROBIOL LETT, V37, P173, DOI 10.1016/0378-1097(86)90399-X; GROSSMAN AR, 1993, MICROBIOL REV, V57, P725, DOI 10.1128/MMBR.57.3.725-749.1993; Harmer SL, 2000, SCIENCE, V290, P2110, DOI 10.1126/science.290.5499.2110; Hastings JW, 1991, NEURAL INTEGRATIVE A, P435; HWANG SB, 1994, PLANT MOL BIOL, V26, P557, DOI 10.1007/BF00013743; JACOBSHAGEN S, 1994, EUR J CELL BIOL, V64, P142; Johnson CH, 2001, ANNU REV PHYSIOL, V63, P695, DOI 10.1146/annurev.physiol.63.1.695; JOHNSON CH, 2001, HDB BEHAV NEUROBIOLO, P61; Kaftan D, 1999, PLANT PHYSIOL, V120, P433, DOI 10.1104/pp.120.2.433; Kawazoe R, 2000, PLANT MOL BIOL, V44, P699, DOI 10.1023/A:1026519718992; Kondo T, 2000, BIOESSAYS, V22, P10, DOI 10.1002/(SICI)1521-1878(200001)22:1<10::AID-BIES4>3.0.CO;2-A; KOSTRZEWA M, 1990, CURR GENET, V18, P465, DOI 10.1007/BF00309918; Lichtle C, 1996, PLANT PHYSIOL, V112, P1045, DOI 10.1104/pp.112.3.1045; LONERGAN TA, 1979, PLANT PHYSIOL, V64, P99, DOI 10.1104/pp.64.1.99; Lopes PF, 2002, BIOCHEM BIOPH RES CO, V295, P50; Lumsden P. J., 1998, BIOL RHYTHMS PHOTOPE; Luning K, 2001, J PHYCOL, V37, P52, DOI 10.1046/j.1529-8817.2001.037001052.x; LUNING K, 1994, J PHYCOL, V30, P190; Makarov VN, 1995, EUR J PHYCOL, V30, P261, DOI 10.1080/09670269500651031; Martin W, 1998, NATURE, V393, P162, DOI 10.1038/30234; McClung CR, 2001, ANNU REV PLANT PHYS, V52, P139, DOI 10.1146/annurev.arplant.52.1.139; MISHKIND M, 1979, PLANT PHYSIOL, V64, P896, DOI 10.1104/pp.64.5.896; Mittag M, 2001, INT REV CYTOL, V206, P213; OKADA M, 1978, PLANT CELL PHYSIOL, V19, P197; Paul JH, 2000, MAR BIOTECHNOL, V2, P429; Piechulla B, 1999, CHRONOBIOL INT, V16, P115, DOI 10.3109/07420529909019080; PIECHULLA B, 1993, PLANT MOL BIOL, V22, P533, DOI 10.1007/BF00015982; PILGRIM ML, 1993, PLANT PHYSIOL, V103, P553, DOI 10.1104/pp.103.2.553; REITH M, 1993, PLANT CELL, V5, P465, DOI 10.1105/tpc.5.4.465; ROELL MK, 1993, PLANT MOL BIOL, V21, P47, DOI 10.1007/BF00039617; ROENNEBERG T, 1993, NATURE, V362, P362, DOI 10.1038/362362a0; Salvador ML, 1998, MOL CELL BIOL, V18, P7235, DOI 10.1128/MCB.18.12.7235; SALVADOR ML, 1993, PLANT J, V3, P213, DOI 10.1046/j.1365-313X.1993.t01-13-00999.x; Sambrook J., 2001, MOL CLONING LAB MANU, V4th; SAMUELSSON G, 1983, PLANT PHYSIOL, V73, P329, DOI 10.1104/pp.73.2.329; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Spreitzer RJ, 2002, ANNU REV PLANT BIOL, V53, P449, DOI 10.1146/annurev.arplant.53.100301.135233; Starr R.C., 1987, Journal of Phycology, V23, P1; Suzuki L, 2001, J PHYCOL, V37, P933, DOI 10.1046/j.1529-8817.2001.01094.x; Thain SC, 2000, CURR BIOL, V10, P951, DOI 10.1016/S0960-9822(00)00630-8; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; Titlyanov EA, 1996, EUR J PHYCOL, V31, P181, DOI 10.1080/09670269600651361a; Toh KL, 2001, SCIENCE, V291, P1040, DOI 10.1126/science.1057499; VALENTIN K, 1990, MOL GEN GENET, V222, P425, DOI 10.1007/BF00633849; VALENTIN K, 1989, CURR GENET, V16, P203, DOI 10.1007/BF00391478; WAALAND SD, 1972, PLANTA, V105, P196, DOI 10.1007/BF00385391; Watson GMF, 1996, PLANT MOL BIOL, V32, P1103, DOI 10.1007/BF00041394; WOOLUM JC, 1991, J BIOL RHYTHM, V6, P129, DOI 10.1177/074873049100600203	62	13	14	0	5	BLACKWELL PUBLISHING INC	MALDEN	350 MAIN ST, MALDEN, MA 02148 USA	0022-3646			J PHYCOL	J. Phycol.	OCT	2003	39	5					888	896		10.1046/j.1529-8817.2003.03022.x			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	725GD	WOS:000185534700008					2021-04-07	
J	Nyvall, P; Corre, E; Boisset, C; Barbeyron, T; Rousvoal, S; Scornet, D; Kloareg, B; Boyen, C				Nyvall, P; Corre, E; Boisset, C; Barbeyron, T; Rousvoal, S; Scornet, D; Kloareg, B; Boyen, C			Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria digitata	PLANT PHYSIOLOGY			English	Article							ECTOCARPUS-SILICULOSUS VIRUS; AZOTOBACTER-VINELANDII; CRYSTAL-STRUCTURE; BETA-HELIX; CATALYTIC DOMAINS; TAILSPIKE PROTEIN; RED ALGAE; SEQUENCE; PHAEOPHYCEAE; ALGINATE	Alginate is an industrially important polysaccharide obtained commercially by harvesting brown algae. The final step in alginate biosynthesis, the epimerization of beta-1,4-D-mannuronic acid to alpha-1,4-L-guluronic acid, a structural change that controls the physicochemical properties of the alginate, is catalyzed by the enzyme mannuronan C-5-epimerase. Six different cDNAs with homology to bacterial mannuronan C-5-epimerases were isolated from the brown alga Laminaria digitata (Phaeophyceae). Hydrophobic cluster analysis indicated that the proteins encoded by the L. digitata sequences have important structural similarities to the bacterial mannuronan C-5-epimerases, including conservation of the catalytic site. The expression of the C-5-epimerase genes was examined by northern-blot analysis and reverse transcriptase-polymerase chain reaction in L. digitata throughout a year. Expression was also monitored in protoplast cultures by northern and western blot, reverse transcriptase-polymerase chain reaction, and activity measurements. From both the structural comparisons and the expression pattern, it appears that the cDNAs isolated from L. digitata encode functional mannuronan C-5-epimerases. The phylogenetic relationships of the bacterial and brown algal enzymes and the inferences on the origin of alginate biosynthetic machinery are discussed.	CNRS, Biol Stn, Unite Mixte Rech 1931, F-29682 Roscoff, Brittany, France; Biol Stn, Lab Goemar, F-29682 Roscoff, Brittany, France	Boyen, C (corresponding author), CNRS, Biol Stn, Unite Mixte Rech 1931, BP 74, F-29682 Roscoff, Brittany, France.	boyen@sb-roscoff.fr	corre, erwan/O-4669-2019	corre, erwan/0000-0001-6354-2278			APT KE, 1995, MOL GEN GENET, V246, P455, DOI 10.1007/BF00290449; Baldauf SL, 2000, SCIENCE, V290, P972, DOI 10.1126/science.290.5493.972; Benet H, 1997, PROTOPLASMA, V199, P39, DOI 10.1007/BF02539804; Benson DA, 1999, NUCLEIC ACIDS RES, V27, P12, DOI 10.1093/nar/27.1.12; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025; BUTLER DM, 1989, J EXP BOT, V40, P1237, DOI 10.1093/jxb/40.11.1237; Callebaut I, 1997, CELL MOL LIFE SCI, V53, P621, DOI 10.1007/s000180050082; Crepineau F, 2000, PLANT MOL BIOL, V43, P503, DOI 10.1023/A:1006489920808; Delaroque N, 2001, VIROLOGY, V287, P112, DOI 10.1006/viro.2001.1028; Douglas SE, 1998, CURR OPIN GENET DEV, V8, P655, DOI 10.1016/S0959-437X(98)80033-6; Ertesvag H, 1998, J BIOL CHEM, V273, P30927, DOI 10.1074/jbc.273.47.30927; ERTESVAG H, 1995, MOL MICROBIOL, V16, P719, DOI 10.1111/j.1365-2958.1995.tb02433.x; ERTESVAG H, 1994, J BACTERIOL, V176, P2846; Ertesvag H, 1999, J BACTERIOL, V181, P3033; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; FITCH WM, 1971, SYST ZOOL, V20, P406, DOI 10.2307/2412116; Freiberg A, 2003, J BIOL CHEM, V278, P1542, DOI 10.1074/jbc.M205294200; Galtier N, 1996, COMPUT APPL BIOSCI, V12, P543; GRASDALEN H, 1983, CARBOHYD RES, V118, P255, DOI 10.1016/0008-6215(83)88053-7; HAUG A, 1974, CARBOHYD RES, V32, P217, DOI 10.1016/S0008-6215(00)82100-X; HELLEBUST JA, 1969, P 6 INT SEAW S, P463; Huang WJ, 1999, J MOL BIOL, V294, P1257, DOI 10.1006/jmbi.1999.3292; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; Lang BF, 1999, J EUKARYOT MICROBIOL, V46, P320, DOI 10.1111/j.1550-7408.1999.tb04611.x; Lietzke SE, 1996, PLANT PHYSIOL, V111, P73, DOI 10.1104/pp.111.1.73; LIN TY, 1966, J BIOL CHEM, V241, P5284; MADGWICK J, 1973, ACTA CHEM SCAND, V27, P3592, DOI 10.3891/acta.chem.scand.27-3592; Michel G, 2001, J BIOL CHEM, V276, P40202, DOI 10.1074/jbc.M100670200; Micheli F, 2001, TRENDS PLANT SCI, V6, P414, DOI 10.1016/S1360-1385(01)02045-3; Micheli F, 1998, GENE, V220, P13, DOI 10.1016/S0378-1119(98)00431-4; Moreira D, 2000, NATURE, V405, P69, DOI 10.1038/35011054; Moulin P, 1999, J PHYCOL, V35, P1237, DOI 10.1046/j.1529-8817.1999.3561237.x; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Nobles DR, 2001, PLANT PHYSIOL, V127, P529, DOI 10.1104/pp.010557; Okasaki M, 1984, JAP J PHYCOL, V32, P364; OKAZAKI M, 1982, BOT MAR, V25, P123, DOI 10.1515/botm.1982.25.3.123; ONSOEN E, 1996, CARBOHYDR EUR, V14, P26; Perez R., 1971, Revue des Travaux, Institut des Peches Maritimes, V35, P287; Petersen TN, 1997, STRUCTURE, V5, P533, DOI 10.1016/S0969-2126(97)00209-8; Rehm BH, 1996, J BACTERIOL, V178, P5884, DOI 10.1128/jb.178.20.5884-5889.1996; Rehm BHA, 1997, APPL MICROBIOL BIOT, V48, P281, DOI 10.1007/s002530051051; Rigden DJ, 2002, FEBS LETT, V530, P225, DOI 10.1016/S0014-5793(02)03490-7; RODDE RSH, 1993, HYDROBIOLOGIA, V261, P577; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Sambrook J., 2001, MOL CLONING LAB MANU, V4th; SITTE P, 1993, EUR J PROTISTOL, V29, P131, DOI 10.1016/S0932-4739(11)80266-X; STEINBACHER S, 1994, SCIENCE, V265, P383, DOI 10.1126/science.8023158; Svanem BIG, 1999, J BACTERIOL, V181, P68, DOI 10.1128/JB.181.1.68-77.1999; Svanem BIG, 2001, J BIOL CHEM, V276, P31542, DOI 10.1074/jbc.M102562200; TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P4350, DOI 10.1073/pnas.76.9.4350; USOV AI, 1995, BOT MAR, V38, P43, DOI 10.1515/botm.1995.38.1-6.43; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; Villand P, 1997, BIOCHEM J, V327, P51, DOI 10.1042/bj3270051	56	53	58	1	11	AMER SOC PLANT BIOLOGISTS	ROCKVILLE	15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA	0032-0889	1532-2548		PLANT PHYSIOL	Plant Physiol.	OCT	2003	133	2					726	735		10.1104/pp.103.025981			10	Plant Sciences	Plant Sciences	732ZE	WOS:000185974800033	14526115	Green Published, Bronze			2021-04-07	
J	Sano, M; Omori, M; Taniguchi, K				Sano, M; Omori, M; Taniguchi, K			Predator-prey systems of drifting seaweed communities off the Tohoku coast, northern Japan, as determined by feeding habit analysis of phytal animals	FISHERIES SCIENCE			English	Article						drifting seaweed; feeding habits; phytal animals; predator-prey system	EARLY-LIFE HISTORY; SEBASTES-THOMPSONI; ROCKFISH; DISPERSAL; FAUNA	We collected phytal animals (fauna associated with drifting seaweeds) off the Tohoku coast in northern Japan in May and June 1994 to examine their feeding habits. Phytal animals were assigned to the following three feeding habit groups: (i) carnivores preying on crustaceans: a gammarid (Stenothoe sp.) and a decapod megalops; (ii) omnivores feeding on crustaceans and pinnate diatoms: a cirripede (Lepas anserifera), three gammarids (Calliopius sp., Jassa slatteryi and J. marmorata), an isopod (Idotea metallica), a decapod crustacean (Planes cyaneus), a decapod megalops and two caprellids (Caprella penantis and Caprella mutica); and (iii) herbivores grazing on pinnate diatoms, fucoids and seaweeds other than fucoids: three gammarids (Allorchestes angusta, Amphithoe lacertosa and Perampithoe lindbergi), an isopod (Cymodoce japonica) and a polychaete (Platynereis bicanaliculata). Pinnate diatoms in the gut contents were composed mainly of Licmophora spp., Navicula spp., Nitzschia spp. and Cocconeis spp. and seaweeds other than fucoids (i.e. Ectocarpus siliculosus and Sphacelaria furcigera). The latter two algae seemed to be epiphytes on drifting seaweeds. These results suggest that phytal animals play an important role in drifting seaweed communities through trophic linkage with two primary producers (i.e. drifting seaweeds and the epiphytes growing on them).	Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan	Sano, M (corresponding author), Japan Soc Promot Sci, Japan Sea Natl Fisheries Res Inst, Fisheries Res Agcy, Niigata 9518121, Japan.	msano@affrc.go.jp					Anraku M, 1965, B SEIKAI REG FISH RE, V33, P13; Endo Yoshinari, 1996, Bulletin of Tohoku National Fisheries Research Institute, V58, P39; GOODING RM, 1967, PAC SCI, V21, P486; Hirosaki Y., 1964, Miscellaneous Reports of the Research Institute for Natural Resources Tokyo, V62, P63; Hunter J. R., 1967, Fishery Bulletin Fish and Wildlife Service US, V66, P13; IDA H, 1967, NIPPON SUISAN GAKK, V33, P930; IDA H, 1967, NIPPON SUISAN GAKK, V33, P923; Ikehara K., 1977, Bulletin Japan Sea reg Fish Res Lab, VNo. 28, P17; INGOLFSSON A, 1995, MAR BIOL, V122, P13, DOI 10.1007/BF00349273; ITO S, 1988, BENTHOS RES, V32, P30; Kawamura T., 1989, B TOHOKU REG FISH R, P41; KIMURA K, 1958, B TOHOKU REG FISH R, V12, P28; KINGSFORD MJ, 1985, LIMNOL OCEANOGR, V30, P618, DOI 10.4319/lo.1985.30.3.0618; Kokita T, 1999, B MAR SCI, V65, P105; Kokita T, 1998, MAR BIOL, V132, P579, DOI 10.1007/s002270050423; NAGASAWA T, 1995, JPN J ICHTHYOL, V41, P385; NUNOMURA N, 1985, B TOYAMA SCI MUS, V7, P51; Round F.E., 1990, DIATOMS; SAFRAN P, 1990, MAR BIOL, V105, P395, DOI 10.1007/BF01316310; Safran Patrick, 1990, Mer (Tokyo), V28, P225; SENTA TETSUSHI, 1962, PHYSIOL AND ECOL [JAPAN], V10, P68; SENTA TETSUSHI, 1966, JAP J ECOL, V16, P165; SHAFFER JA, 1995, MAR ECOL PROG SER, V123, P13, DOI 10.3354/meps123013; SHOUJIMA Y, 1964, NIPPON SUISAN GAKK, V30, P248; Tanaka N, 1986, B NATL RES I AQUACUL, V9, P59; Tsukidate J, 1978, B NANSEI REG FISH RE, V11, P33; UCHIDA K., 1958, BULL JAPANESE SOC SCI FISH, V24, P411; Yoshida T., 1963, B TOHOKU REG FISH R, V23, P141	28	37	43	0	16	SPRINGER JAPAN KK	TOKYO	CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN	0919-9268	1444-2906		FISHERIES SCI	Fish. Sci.	APR	2003	69	2					260	268		10.1046/j.1444-2906.2003.00616.x			9	Fisheries	Fisheries	676DY	WOS:000182718700006					2021-04-07	
J	Klenell, M; Snoeijs, P; Pedersen, M				Klenell, M; Snoeijs, P; Pedersen, M			The involvement of a plasma membrane H+-ATPase in the blue-light enhancement of photosynthesis in Laminaria digitata (Phaeophyta)	JOURNAL OF PHYCOLOGY			English	Article						acetazolamide; blue-light effect; brown algae; carbon uptake; Laminaria digitata; vanadate	CARBONIC-ANHYDRASE ACTIVITY; BROWN-ALGAE; SATURATED PHOTOSYNTHESIS; GUARD-CELLS; CHLAMYDOMONAS-REINHARDTII; ECTOCARPUS-SILICULOSUS; ACQUISITION; STIMULATION; INHIBITORS; VANADATE	Many brown algae, including the kelp Laminaria digitata (Huds.) Lamour., exhibit enhanced photosynthesis when they are given a small amount of blue-light in addition to a background of saturating red light. This blue light effect is correlated with an increased uptake of carbon. In the present study, we tested the hypothesis that blue light acts by increasing the activity of a plasma membrane H (+) -ATPase, thereby promoting an active carbon uptake across the plasma membrane. Photosynthetic carbon uptake was studied in pH-drift experiments under illumination with red and blue light and using different inhibitors. Vanadate, an inhibitor of plasma membrane H (+) -ATPases, had a minor inhibitory effect on carbon uptake rates under saturating red light conditions, but inhibited the blue-light enhancement by approximately 60%. An inhibitor of external carbonic anhydrase, acetazolamide, decreased the carbon uptake in both red light and in red plus blue light by 48% and 68%, respectively. These results suggest that photosynthetic carbon uptake depends on an external carbonic anhydrase under both red and red plus blue light conditions, and that blue light induces an increased activity of a P-type H (+) -ATPase in the plasma membrane. The proton buffer Tris, which has a buffering capacity similar to vanadate in seawater, had no inhibitory effect on carbon uptake rates neither in red light nor in red plus blue light, showing that the inhibitory effect of vanadate is not caused by its effect as a buffer. The blue-light enhancement was also abolished by a protein kinase inhibitor (H-7), suggesting that the transduction of the blue-light signal involves a protein kinase, which activates the plasma membrane H (+) -ATPase by phosphorylation.	Stockholm Univ, Dept Bot, SE-10691 Stockholm, Sweden; Stockholm Univ, Dept Bot, SE-10691 Stockholm, Sweden; Uppsala Univ, Evolutionary Biol Ctr, Dept Plant Ecol, SE-75236 Uppsala, Sweden	Pedersen, M (corresponding author), Stockholm Univ, Dept Bot, SE-10691 Stockholm, Sweden.						AMODEO G, 1992, PLANT PHYSIOL, V100, P1567, DOI 10.1104/pp.100.3.1567; Axelsson L, 2000, EUR J PHYCOL, V35, P53, DOI 10.1080/09670260010001735621; BEFFAGNA N, 1988, J EXP BOT, V39, P1033, DOI 10.1093/jxb/39.8.1033; Busch S, 2001, EUR J PHYCOL, V36, P61, DOI 10.1080/09670260110001735208; Choo KS, 2002, J PHYCOL, V38, P493, DOI 10.1046/j.1529-8817.2002.t01-1-01083.x; Cousson A, 2001, PLANT SCI, V161, P249, DOI 10.1016/S0168-9452(01)00348-X; DIONISIO ML, 1989, PLANT CELL PHYSIOL, V30, P215, DOI 10.1093/oxfordjournals.pcp.a077732; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; Franklin LA, 2001, J PHYCOL, V37, P257, DOI 10.1046/j.1529-8817.2001.037002257.x; GALLAGHER SR, 1982, PLANT PHYSIOL, V70, P1335, DOI 10.1104/pp.70.5.1335; GILMOUR DJ, 1985, J PLANT PHYSIOL, V118, P111, DOI 10.1016/S0176-1617(85)80140-1; Giraldez N, 2000, J EXP BOT, V51, P807, DOI 10.1093/jexbot/51.345.807; Granbom M, 1999, HYDROBIOLOGIA, V399, P349; HAGLUND K, 1992, PLANTA, V188, P1, DOI 10.1007/BF00198932; HENLEY WJ, 1993, J PHYCOL, V29, P729, DOI 10.1111/j.0022-3646.1993.00729.x; HIDAKA H, 1984, BIOCHEMISTRY-US, V23, P5036, DOI 10.1021/bi00316a032; Hillrichs S, 2001, EUR J PHYCOL, V36, P71, DOI 10.1017/S096702620100302X; Jarillo JA, 2001, NATURE, V410, P952, DOI 10.1038/35073622; JASSBY AD, 1976, LIMNOL OCEANOGR, V21, P540, DOI 10.4319/lo.1976.21.4.0540; Jin X, 2001, J EXP BOT, V52, P91, DOI 10.1093/jexbot/52.354.91; Kinoshita T, 1999, EMBO J, V18, P5548, DOI 10.1093/emboj/18.20.5548; Kinoshita T, 2001, PLANT CELL PHYSIOL, V42, P424, DOI 10.1093/pcp/pce055; KLENELL M, 2003, IN PRESS HYDROBIOLOG; MORONEY JV, 1985, PLANT PHYSIOL, V79, P177, DOI 10.1104/pp.79.1.177; Nimer NA, 1998, PLANT CELL ENVIRON, V21, P805, DOI 10.1046/j.1365-3040.1998.00321.x; Nimer NA, 1999, J PHYCOL, V35, P1200, DOI 10.1046/j.1529-8817.1999.3561200.x; Provasoli L., 1968, CULTURES COLLECTIONS, P63; Quinones MA, 1997, PHYSIOL PLANTARUM, V100, P45; RAMAZANOV Z, 1995, PLANTA, V195, P519, DOI 10.1007/BF00195709; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; Schmid R, 1996, PLANT CELL ENVIRON, V19, P383, DOI 10.1111/j.1365-3040.1996.tb00330.x; Schmid R, 1996, SCI MAR, V60, P115; Snoeijs P, 2002, MAR BIOL, V140, P435, DOI 10.1007/s00227-001-0729-x; SRIVASTAVA A, 1995, PLANTA, V196, P445, DOI 10.1007/BF00203642; Zeiger E, 1998, J EXP BOT, V49, P433, DOI 10.1093/jexbot/49.suppl_1.433; Zeiger E, 2000, TRENDS PLANT SCI, V5, P183, DOI 10.1016/S1360-1385(00)01602-2	40	16	21	0	2	BLACKWELL PUBLISHING INC	MALDEN	350 MAIN ST, MALDEN, MA 02148 USA	0022-3646			J PHYCOL	J. Phycol.	DEC	2002	38	6					1143	1149		10.1046/j.1529-8817.2002.02063.x			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	627CG	WOS:000179910900010					2021-04-07	
J	Xu, YN; Wang, ZN; Yan, XJ; Lin, W; Li, LB; Kuang, TY				Xu, YN; Wang, ZN; Yan, XJ; Lin, W; Li, LB; Kuang, TY			Positional distribution of fatty acids on the glycerol backbone during the biosynthesis of glycerolipids in Ectocarpus fasciculatus	CHINESE SCIENCE BULLETIN			English	Article						E fasciculatus; glycerolipid; fatty acid; isotope label	LIPID-METABOLISM; PHOSPHATIDYLGLYCEROL; BIOCHEMISTRY; PHAEOPHYCEAE; SULFOLIPIDS	The biosynthesis of glycolipids in E. fasciculatus was studied by C-14 label and chase. The fatty acids in sulphoquinovosyl diacylglycerol (SQDG) were almost 16-carbon and 18-carbon ones. In addition to the two fatty acids, monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) contained 8.5 mol% and 31.0 mol% of eicosapentaenoic acid (20 : 5), respectively, and this fatty acid was usually distributed in the sn-1 position of the glycerol backbone. When plants were incubated with [2-C-14] acetate, differences existed in the positional distribution of the labeled fatty acids in sn-1 and sn-2 among the three glycerolipids. In SQDG C-14-labeled fatty acids were distributed uniformly in the sn-1 and sn-2 positions. In DGDG, C-14-labeled fatty acids were mainly distributed in the sn-2 position. In MGDG, the radioactivity of fatty acids in sn-1 position was far greater than that in sn-2 position after a 30 min pulse label, and the difference in radioactivity between the two positions decreased rapidly. The above results indicated that differences in the positional distribution of C-14-labeled fatty acids between sn-1 and sn-2 positions might be related to 20 : 5 and the biosynthesis of DGDG. Our results also suggested that E. fasciculatus had the same DGDG biosynthetic pathway as that in higher plants and galactosyl transferase was selective for MGDC.	Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China; Chinese Acad Sci, Inst Marine Biol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China	Xu, YN (corresponding author), Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China.						BROWSE J, 1991, ANNU REV PLANT PHYS, V42, P467, DOI 10.1146/annurev.pp.42.060191.002343; DORNE AJ, 1990, P NATL ACAD SCI USA, V87, P71, DOI 10.1073/pnas.87.1.71; DOUCE R, 1990, ANNU REV CELL BIOL, V6, P173, DOI 10.1146/annurev.cb.06.110190.001133; FISCHER W, 1973, H-S Z PHYSIOL CHEM, V354, P1115, DOI 10.1515/bchm2.1973.354.2.1115; GUSTAFSON KR, 1989, J NATL CANCER I, V81, P1254, DOI 10.1093/jnci/81.16.1254; HARWOOD JL, 1998, MEMBRANE LIPIDS ALGA, P53; HEINZ E, 1983, PLANT PHYSIOL, V72, P273, DOI 10.1104/pp.72.2.273; HEINZ E, 1993, RECENT INVESTIGATION, P163; Makewicz A, 1997, PLANT CELL PHYSIOL, V38, P952, DOI 10.1093/oxfordjournals.pcp.a029257; Murata N, 1998, LIPIDS PHOTOSYNTHESI, V6, P1, DOI DOI 10.1007/0-306-48087-51; Ohta K, 1999, BIOL PHARM BULL, V22, P111, DOI 10.1248/bpb.22.111; Shiran D, 1996, LIPIDS, V31, P1277, DOI 10.1007/BF02587913; XU Y, 1998, SECRETARIADO PUBLICA, P232; Xu YN, 1997, PLANT CELL PHYSIOL, V38, P611, DOI 10.1093/oxfordjournals.pcp.a029211; Xu YN, 1999, FETT-LIPID, V101, P104	15	13	13	0	5	SCIENCE CHINA PRESS	BEIJING	16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA	1001-6538			CHINESE SCI BULL	Chin. Sci. Bull.	NOV	2002	47	21					1802	1806		10.1360/02tb9394			5	Multidisciplinary Sciences	Science & Technology - Other Topics	606VC	WOS:000178754200010					2021-04-07	
J	Kupper, H; Setlik, I; Spiller, M; Kupper, FC; Prasil, O				Kupper, H; Setlik, I; Spiller, M; Kupper, FC; Prasil, O			Heavy metal-induced inhibition of photosynthesis: Targets of in vivo heavy metal chlorophyll formation	JOURNAL OF PHYCOLOGY			English	Article						Chlorophyta; copper; heavy metal substituted chlorophyll; Phaeophyta; photosynthesis; Rhodophyta; zinc	CU(II)-INHIBITORY BINDING-SITE; PHOTOSYSTEM-II; ELECTRON-TRANSPORT; HIGHER-PLANTS; BROWN ALGA; SUBSTITUTED CHLOROPHYLLS; THYLAKOID MEMBRANES; FUCUS-VESICULOSUS; OXIDATIVE DAMAGE; WATER PLANTS	The targets of heavy metal (here Cu2+ and Zn2+) attack on the photosynthetic apparatus of algae belonging to different phyla were investigated. Experiments with the green alga Scenedesmus quadricauda confirmed previous findings that according to the irradiance level two different phenomena occur, which were further characterized by specific changes in several photosynthetic parameters. The reaction occurring under low irradiance (shade reaction) is characterized by heavy metal substitution of Mg2+ in chl molecules bound predominantly in the light harvesting complex II of Chlorophyta (LHC II). Under high irradiance (sun reaction) the LHC II chls are inaccessible to substitution and the damage occurs in the PSII reaction center instead. Algae with antenna proteins other than the LHC II did not show the two types of heavy metal attack at different irradiances. In red algae (Antithamnion plumula) , low Cu2+ concentrations induced the sun reaction even at very low irradiance. In brown algae (Ectocarpus siliculosus ) the shade reaction occurred even in saturating irradiance. These results also indicate that despite some similarity in their features, the primary step of the sun reaction and photoinhibition is different.	Univ Konstanz, Math Nat Wissensch Sekt, Fachbereich Biol, D-78457 Constance, Germany; Inst Microbiol, Photosynth Res Ctr, CZ-37981 Trebon, Czech Republic; Univ S Bohemia, CZ-37981 Trebon, Czech Republic; Rhein Westfal TH Aachen, Inst Wasserbau & Wasserwirtschaft, D-52056 Aachen, Germany; Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA	Kupper, H (corresponding author), Oderbruchstr 27, D-45770 Marl, Germany.		Kupper, Hendrik/J-5152-2012; Prasil, Ondrej/H-2454-2014	Kupper, Hendrik/0000-0003-0712-7023; Prasil, Ondrej/0000-0002-0012-4359; Kuepper, Frithjof/0000-0003-1273-7109			ABELEOESCHGER D, 1991, J EXP MAR BIOL ECOL, V147, P177, DOI 10.1016/0022-0981(91)90181-U; AHMED A, 1993, J INORG BIOCHEM, V50, P235, DOI 10.1016/0162-0134(93)80050-J; ATAL N, 1991, PLANT CELL PHYSIOL, V32, P943, DOI 10.1093/oxfordjournals.pcp.a078181; BARON M, 1995, PHYSIOL PLANTARUM, V94, P174, DOI 10.1111/j.1399-3054.1995.tb00799.x; BARTOS J, 1975, PHOTOSYNTHETICA, V9, P395; BEREZIN BD, 1970, RUSS J PHYS CH USSR, V44, P1597; BOUCHER LJ, 1967, J AM CHEM SOC, V89, P4703, DOI 10.1021/ja00994a024; Boucher N, 1999, PHOTOSYNTH RES, V59, P167, DOI 10.1023/A:1006194621553; CEDENOMALDONADO A, 1972, PLANT PHYSIOL, V50, P698, DOI 10.1104/pp.50.6.698; CLIJSTERS H, 1985, PHOTOSYNTH RES, V7, P31, DOI 10.1007/BF00032920; Clijsters H, 1999, Z NATURFORSCH C, V54, P730; DEFILIPPIS LF, 1979, Z PFLANZENPHYSIOL, V93, P129, DOI 10.1016/S0044-328X(79)80052-5; Durnford DG, 1999, J MOL EVOL, V48, P59, DOI 10.1007/PL00006445; FERNANDES JC, 1991, BOT REV, V57, P246, DOI 10.1007/BF02858564; Fiedor L, 2001, BIOCHEMISTRY-US, V40, P3737, DOI 10.1021/bi002257f; FOSTER PL, 1977, NATURE, V269, P322, DOI 10.1038/269322a0; Gledhill M, 1999, J PHYCOL, V35, P501, DOI 10.1046/j.1529-8817.1999.3530501.x; Green BR, 1996, ANNU REV PLANT PHYS, V47, P685, DOI 10.1146/annurev.arplant.47.1.685; GROSS R E, 1970, Plant Physiology (Rockville), V46, P183, DOI 10.1104/pp.46.2.183; Hartwich G, 1998, J AM CHEM SOC, V120, P3675, DOI 10.1021/ja970874u; HSU BD, 1988, PLANT PHYSIOL, V87, P116, DOI 10.1104/pp.87.1.116; JEGERSCHOLD C, 1995, BIOCHEMISTRY-US, V34, P12747, DOI 10.1021/bi00039a034; JONES AL, 1993, J EXP BOT, V44, P1203, DOI 10.1093/jxb/44.7.1203; KATOH T, 1990, PLANT CELL PHYSIOL, V31, P439; KIMIMURA M, 1972, BIOCHIM BIOPHYS ACTA, V283, P279, DOI 10.1016/0005-2728(72)90244-7; KNEER R, 1992, PHYTOCHEMISTRY, V31, P2663, DOI 10.1016/0031-9422(92)83607-Z; KOWALEWSKA G, 1989, ACTA PHYSIOL PLANT, V11, P39; Kowalewska Grazyna, 1992, Polskie Archiwum Hydrobiologii, V39, P41; KRUPA Z, 1988, PHYSIOL PLANTARUM, V73, P518, DOI 10.1111/j.1399-3054.1988.tb05435.x; Krupa Z, 1999, Z NATURFORSCH C, V54, P723; KRUPA Z, 1987, PHOTOSYNTHETICA, V21, P156; Kupper H, 2000, ANAL BIOCHEM, V286, P247, DOI 10.1006/abio.2000.4794; Kupper H, 1996, J EXP BOT, V47, P259, DOI 10.1093/jxb/47.2.259; Kupper H, 1998, PHOTOSYNTH RES, V58, P123, DOI 10.1023/A:1006132608181; LUNA CM, 1994, PLANT CELL PHYSIOL, V35, P11; Maeda S., 1990, INTRO APPL PHYCOLOGY; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; MIMURO M, 1990, BIOCHIM BIOPHYS ACTA, V1015, P450, DOI 10.1016/0005-2728(90)90078-I; Okamoto OK, 1996, J PHYCOL, V32, P74, DOI 10.1111/j.0022-3646.1996.00074.x; PAULSEN H, 1993, EUR J BIOCHEM, V215, P809, DOI 10.1111/j.1432-1033.1993.tb18096.x; Prasil O, 1996, PHOTOSYNTH RES, V48, P395, DOI 10.1007/BF00029472; PUCKETT KJ, 1976, CAN J BOT, V54, P2695, DOI 10.1139/b76-290; REBEIZ CA, 1984, SPECTROCHIM ACTA A, V40, P793, DOI 10.1016/0584-8539(84)80170-1; SAMSON G, 1988, PHOTOCHEM PHOTOBIOL, V48, P329, DOI 10.1111/j.1751-1097.1988.tb02829.x; SCHANDERL SH, 1965, J FOOD SCI, V30, P312, DOI 10.1111/j.1365-2621.1965.tb00308.x; SCHRODER WP, 1994, J BIOL CHEM, V269, P32865; SETLIK I, 1972, Algological Studies, V7, P172; SETLIKOVA E, 1995, PHOTOSYNTH RES, V43, P201, DOI 10.1007/BF00029933; SHEORAN IS, 1990, PHOTOSYNTH RES, V23, P345, DOI 10.1007/BF00034865; SOFROVA D, 1992, PHOTOSYNTHETICA, V26, P79; STOBART AK, 1985, PHYSIOL PLANTARUM, V63, P293, DOI 10.1111/j.1399-3054.1985.tb04268.x; Strasser RJ, 2000, FLUORESCENCE TRANSIE, P445, DOI DOI 10.1134/S0006297914040014; VAVILIN DV, 1995, J PLANT PHYSIOL, V146, P609, DOI 10.1016/S0176-1617(11)81922-X; Weckx JEJ, 1996, PHYSIOL PLANTARUM, V96, P506, DOI 10.1111/j.1399-3054.1996.tb00465.x; WU JT, 1984, BOT BULL ACAD SINICA, V25, P125; Xiong FS, 1997, PHYSIOL PLANTARUM, V100, P378; YRUELA I, 1992, PHOTOSYNTH RES, V33, P227, DOI 10.1007/BF00030033; YRUELA I, 1991, J BIOL CHEM, V266, P22847; Yruela I, 1996, BIOCHEMISTRY-US, V35, P9469, DOI 10.1021/bi951667e; YRUELA I, 1993, J BIOL CHEM, V268, P1684; ZOLOTUKHINA EY, 1995, VEST MOSKOVSK U S 16, V71, P46	61	250	274	4	65	BLACKWELL PUBLISHING INC	MALDEN	350 MAIN ST, MALDEN, MA 02148 USA	0022-3646			J PHYCOL	J. Phycol.	JUN	2002	38	3					429	441		10.1046/j.1529-8817.2002.01148.x			13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	563AY	WOS:000176232300003					2021-04-07	
J	Gaylord, B; Reed, DC; Raimondi, PT; Washburn, L; McLean, SR				Gaylord, B; Reed, DC; Raimondi, PT; Washburn, L; McLean, SR			A physically based model of macroalgal spore dispersal in the wave and current-dominated nearshore	ECOLOGY			English	Article						Ectocarpus siliculosus; eddy diffusivity; flow; macroalgae; Macrocystis pyrifera; propagule; release height; Sarcodiotheca gaudichaudii; Sargassum muticum; sinking speed; turbulence; vertical mixing	KELPS MACROCYSTIS-PYRIFERA; SPATIALLY AVERAGED FLOW; PTERYGOPHORA-CALIFORNICA; TURBULENT TRANSPORT; SUSPENDED PARTICLES; SEAWEED PROPAGULES; MARINE MACROALGAE; CONTINENTAL-SHELF; BENTHIC ORGANISMS; SETTLEMENT	Propagule dispersal in seaweeds is a process influenced by a variety of biological and physical factors, the complexity of which has hindered efforts to understand colonization, persistence, post-disturbance recovery, and dynamics of algal populations in general. In view of this limitation, we employ here modifications to an existing turbulent-transport model to explore the mechanics of nearshore macroalgal spore dispersal and its relationship to coastal hydrodynamic conditions, Our modeling efforts focus on four example species of seaweed whose reproductive propagules span a wide range in sinking speed and height of release above the sea floor: the giant kelp Macrocystis pyrifera, the erect fucoid Sargassum muticum, the small Filamentous brown alga Ectocarpus siliculosus, and the flaccid red alga Sarcodiotheca gaudichaudii. Results indicate that both propagule sinking speed and release height can affect dispersal distance substantially, but that the influence of these biological parameters is modulated strongly by the intensity of turbulence as dictated by waves and currents. In rapid flows with larger waves, it is primarily fluid dynamic processes, in particular current velocities, that determine dispersal distance. Additional Simulations suggest that patterns of spore dispersal are highly skewed, with most propagules encountering the sea floor within a few meters to hundreds of meters of their parents, but with a sizeable fraction of spores also dispersing as far as kilometers. Such model predictions imply a much greater potential for longer range dispersal than has typically been assumed, a finding with important implications for understanding the demographics of algal populations and for predicting levels of connectivity among them.	Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA; Univ Calif Santa Barbara, Inst Marine Sci, Santa Barbara, CA 93106 USA; Univ Calif Santa Cruz, Dept Biol, Santa Cruz, CA 95064 USA; Univ Calif Santa Barbara, Dept Geog, Inst Computat Earth Syst Sci, Santa Barbara, CA 93106 USA; Univ Calif Santa Barbara, Dept Mech & Environm Engn, Santa Barbara, CA 93106 USA	Gaylord, B (corresponding author), Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA.	gaylord@lifesci.ucsb.edu					Abbott I. A., 1976, MARINE ALGAE CALIFOR; ACKERMAN JD, 1993, FUNCT ECOL, V7, P305, DOI 10.2307/2390209; AMSLER CD, 1991, J PHYCOL, V27, P26, DOI 10.1111/j.0022-3646.1991.00026.x; AMSLER CD, 1992, BRIT PHYCOL J, V27, P253, DOI 10.1080/00071619200650251; AMSLER CD, 1980, J PHYCOL, V16, P617, DOI 10.1111/j.1529-8817.1980.tb03080.x; AMSLER CD, 1989, MAR BIOL, V102, P557, DOI 10.1007/BF00438358; Amsler CD, 1999, J PHYCOL, V35, P239, DOI 10.1046/j.1529-8817.1999.3520239.x; Anderson EK, 1966, P INT SEAWEED S, V5, P73; Berg H., 1983, RANDOM WALKS BIOL; BUTMAN CA, 1988, NATURE, V333, P771, DOI 10.1038/333771a0; CHAPMAN ARO, 1986, ADV MAR BIOL, V23, P1; CLAYTON MN, 1992, BRIT PHYCOL J, V27, P219, DOI 10.1080/00071619200650231; COON D, 1972, P INT SEAWEED S, V7, P237; Davies AG, 1997, COAST ENG, V31, P163, DOI 10.1016/S0378-3839(97)00005-7; DAYTON PK, 1985, ANNU REV ECOL SYST, V16, P215, DOI 10.1146/annurev.es.16.110185.001243; DAYTON PK, 1984, ECOL MONOGR, V54, P253, DOI 10.2307/1942498; DENNY MW, 1985, LIMNOL OCEANOGR, V30, P1171, DOI 10.4319/lo.1985.30.6.1171; DENNY MW, 1989, AM NAT, V134, P859, DOI 10.1086/285018; DENNY MW, 1993, AIR WATER BIOL PHYSI; DENNY MW, 1988, BIOL MECH WAVESWEPT; DESTOMBE C, 1992, BOT MAR, V35, P93, DOI 10.1515/botm.1992.35.2.93; ECKMAN JE, 1990, LIMNOL OCEANOGR, V35, P887, DOI 10.4319/lo.1990.35.4.0887; ECKMAN JE, 1989, J EXP MAR BIOL ECOL, V129, P173, DOI 10.1016/0022-0981(89)90055-5; Ferziger J.H., 1981, NUMERICAL METHODS EN; FOSTER MS, 1993, P 2 INT TEMP REEF S, P151; FREDRIKSEN S, 1995, SARSIA, V80, P47, DOI 10.1080/00364827.1995.10413579; Gaylord B, 1997, J EXP BIOL, V200, P3141; Gaylord B, 1999, J EXP MAR BIOL ECOL, V239, P85, DOI 10.1016/S0022-0981(99)00031-3; GRANT WD, 1986, ANNU REV FLUID MECH, V18, P265, DOI 10.1146/annurev.fl.18.010186.001405; GRANT WD, 1979, J GEOPHYS RES-OCEANS, V84, P1797, DOI 10.1029/JC084iC04p01797; GROSS TF, 1992, J MAR RES, V50, P611, DOI 10.1357/002224092784797575; HOFFMANN AJ, 1989, J EXP MAR BIOL ECOL, V126, P281, DOI 10.1016/0022-0981(89)90193-7; HOFFMANN AJ, 1987, BOT MAR, V30, P151, DOI 10.1515/botm.1987.30.2.151; HOFFMANN AJ, 1985, J EXP MAR BIOL ECOL, V92, P83, DOI 10.1016/0022-0981(85)90023-1; JONNSON S, 1972, SURTSEY RES PROGR RE, V6, P75; KEOUGH MJ, 1989, P INT COR REEF S AUS, V6, P141; Mann KH, 1996, DYNAMICS MARINE ECOS; McLean SR, 1999, J GEOPHYS RES-OCEANS, V104, P15743, DOI 10.1029/1999JC900116; McNair JN, 2000, J THEOR BIOL, V202, P231, DOI 10.1006/jtbi.1999.1057; McNair JN, 1997, J THEOR BIOL, V188, P29, DOI 10.1006/jtbi.1997.0453; MIDDLETON GV, 1984, LECT NOTES SPONSERED; MILEIKOVSKY SA, 1973, MAR BIOL, V23, P11, DOI 10.1007/BF00394107; NORTON TA, 1992, BRIT PHYCOL J, V27, P293, DOI 10.1080/00071619200650271; NORTON TA, 1981, J MAR BIOL ASSOC UK, V61, P929, DOI 10.1017/S0025315400023067; RAIMONDI PT, 1990, AUST J ECOL, V15, P427, DOI 10.1111/j.1442-9993.1990.tb01468.x; REED DC, 1991, J PHYCOL, V27, P361, DOI 10.1111/j.0022-3646.1991.00361.x; REED DC, 1992, ECOLOGY, V73, P1577, DOI 10.2307/1940011; REED DC, 1988, ECOL MONOGR, V58, P321, DOI 10.2307/1942543; Reed DC, 1997, ECOLOGY, V78, P2443; REED DC, 1990, ECOLOGY, V71, P2286, DOI 10.2307/1938639; Reed DC, 1999, MAR BIOL, V133, P737, DOI 10.1007/s002270050515; RICHMOND RH, 1987, MAR BIOL, V93, P527, DOI 10.1007/BF00392790; SANTELICES B, 1990, OCEANOGR MAR BIOL, V28, P177; Scheltema R. S., 1971, 4TH EUR MAR BIOL S, P7; SCHIEL DR, 1986, OCEANOGR MAR BIOL, V24, P265; SMITH JD, 1977, J GEOPHYS RES-OC ATM, V82, P1735, DOI 10.1029/JC082i012p01735; VANDENHOEK C, 1987, HELGOLANDER MEERESUN, V41, P261; Vogel S, 1994, LIFE MOVING FLUIDS; Washburn L, 1999, CONT SHELF RES, V19, P57, DOI 10.1016/S0278-4343(98)00068-5; WIBERG P, 1983, CONT SHELF RES, V2, P147, DOI 10.1016/0278-4343(83)90013-4; ZECHMAN FW, 1985, BOT MAR, V28, P283, DOI 10.1515/botm.1985.28.7.283	61	143	144	2	57	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0012-9658	1939-9170		ECOLOGY	Ecology	MAY	2002	83	5					1239	1251		10.2307/3071939			13	Ecology	Environmental Sciences & Ecology	543YK	WOS:000175131300008					2021-04-07	
J	Granbom, M; Pedersen, M; Kadel, P; Luning, K				Granbom, M; Pedersen, M; Kadel, P; Luning, K			Circadian rhythm of photosynthetic oxygen evolution in Kappaphycus alvarezii (Rhodophyta): Dependence on light quantity and quality	JOURNAL OF PHYCOLOGY			English	Article						circadian rhythm; irradiance; Kappaphycus; photosynthesis; Rhodophyta; spectral demands	BLUE-LIGHT; ECTOCARPUS PHAEOPHYTA; GONYAULAX-POLYEDRA; MOLECULAR-BASES; FAST RESPONSES; CLOCK; CRYPTOCHROMES; ARABIDOPSIS; PERIOD; PHYTOCHROMES	The rate of oxygen evolution of the tropical red alga Kappaphycus alvarezii (Doty) Doty was measured for 6 days in the laboratory using a computer-aided method for long-term recording. In cool white light, Kappaphycus exhibited a robust circadian rhythm of O-2 evolution in the irradiance range of 100 to 1000 mu mol photons(.)m(-2.)s(-1). With increasing irradiance, the period of the free-running rhythm, tau, decreased in blue and increased in red light but did not change significantly in green light. The accelerating or slowing action of blue or red light, respectively, points to two photoreceptors used in the light transduction pathway of the circadian oscillator controlling oxygen evolution or the light reactions of photosynthesis in Kappaphycus. No significant changes of tau were observed with increasing irradiance in cool white light, possibly due to the additive opposing responses caused by blue and red light.	Stockholm Univ, Dept Bot, SE-10691 Stockholm, Sweden; Alfred Wegener Inst Polar & Marine Res, Wattenmeerstn Sylt, D-25992 List Auf Sylt, Germany	Granbom, M (corresponding author), Stockholm Univ, Dept Bot, SE-10691 Stockholm, Sweden.						ASCHOFF J, 1979, Z TIERPSYCHOL, V49, P225; Aschoff J, 1981, BIOL RHYTHMS, P81; BRITZ SJ, 1976, PLANT PHYSIOL, V58, P22, DOI 10.1104/pp.58.1.22; Casal JJ, 2000, PHOTOCHEM PHOTOBIOL, V71, P1, DOI 10.1562/0031-8655(2000)071&lt;0001:PCPPII&gt;2.0.CO;2; Cashmore AR, 1999, SCIENCE, V284, P760, DOI 10.1126/science.284.5415.760; DOTY M S, 1973, Micronesica, V9, P59; DOTY MS, 1987, CASE STUDIES 7 COMME, P123; DRIESSCHE TV, 1966, EXP CELL RES, V42, P18; DRING MJ, 1967, NATURE, V215, P1411, DOI 10.1038/2151411a0; Dunlap JC, 1999, CELL, V96, P271, DOI 10.1016/S0092-8674(00)80566-8; Edmunds LN, 1988, CELLULAR MOL BASES B; ENRIGHT JT, 1965, J THEOR BIOL, V8, P426, DOI 10.1016/0022-5193(65)90021-4; FREDEEN AL, 1991, PLANT PHYSIOL, V97, P415, DOI 10.1104/pp.97.1.415; GREEN EJ, 1967, J MAR RES, V25, P140; Guillard R. L. L., 1975, CULTURE MARINE INVER, P29, DOI DOI 10.1007/978-1-4615-8714-9_3; GUILLARD RR, 1962, CAN J MICROBIOL, V8, P229, DOI 10.1139/m62-029; HARRIS PJC, 1978, PLANTA, V138, P271, DOI 10.1007/BF00386822; HASTINGS JW, 1961, J GEN PHYSIOL, V45, P69, DOI 10.1085/jgp.45.1.69; Hastings JW, 1991, NEURAL INTEGRATIVE A, P435; HUPPERTZ K, 1990, MAR ECOL PROG SER, V66, P175, DOI 10.3354/meps066175; Jerlov N.G., 1976, MARINE OPTICS; Johnson C.H., 1998, BIOL RHYTHMS PHOTOPE, P1; KAGEYAMA A, 1979, BOT MAR, V22, P199, DOI 10.1515/botm.1979.22.4.199; KONDO T, 1993, P NATL ACAD SCI USA, V90, P5672, DOI 10.1073/pnas.90.12.5672; LAVALMARTIN DL, 1979, PLANT PHYSIOL, V63, P495, DOI 10.1104/pp.63.3.495; LOPEZFIGUEROA F, 1992, BOT ACTA, V102, P178; LUNING K, 1980, J PHYCOL, V16, P1; Luning K, 2001, J PHYCOL, V37, P52, DOI 10.1046/j.1529-8817.2001.037001052.x; MILLAR AJ, 1995, SCIENCE, V267, P1163, DOI 10.1126/science.7855596; MISHKIND M, 1979, PLANT PHYSIOL, V64, P896, DOI 10.1104/pp.64.5.896; OKADA M, 1978, PLANT CELL PHYSIOL, V19, P197; OOHUSA T, 1980, BOT MAR, V23, P1, DOI 10.1515/botm.1980.23.1.1; Perez R., 1992, CULTURE ALGUES MARIN; Roenneberg T, 1996, PHYSIOL PLANTARUM, V96, P733, DOI 10.1111/j.1399-3054.1996.tb00250.x; Roenneberg T, 1996, FASEB J, V10, P1443; ROENNEBERG T, 1988, NATURWISSENSCHAFTEN, V75, P206, DOI 10.1007/BF00735584; SAMUELSSON G, 1983, PLANT PHYSIOL, V73, P329, DOI 10.1104/pp.73.2.329; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SOKOLOVE PG, 1978, J THEOR BIOL, V72, P131, DOI 10.1016/0022-5193(78)90022-X; Somers DE, 1999, PLANT PHYSIOL, V121, P9, DOI 10.1104/pp.121.1.9; Somers DE, 1998, DEVELOPMENT, V125, P485; Somers DE, 1998, SCIENCE, V282, P1488, DOI 10.1126/science.282.5393.1488; Starr R.C., 1987, Journal of Phycology, V23, P1; SWEENEY BM, 1987, RHYTMIC PHENOMENA PL	45	23	23	0	6	BLACKWELL PUBLISHING INC	MALDEN	350 MAIN ST, MALDEN, MA 02148 USA	0022-3646			J PHYCOL	J. Phycol.	DEC	2001	37	6					1020	1025		10.1046/j.1529-8817.2001.01040.x			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	507LG	WOS:000173029500013					2021-04-07	
J	Muyssen, BTA; Janssen, CR				Muyssen, BTA; Janssen, CR			Zinc acclimation and its effect on the zinc tolerance of Raphidocelis subcapitata and Chlorella vulgaris in laboratory experiments	CHEMOSPHERE			English	Article						zinc; green algae; toxicity; deficiency; acclimation	HEAVY-METAL TOLERANCE; ALGA ECTOCARPUS-SILICULOSUS; UNICELLULAR GREEN-ALGA; SELENASTRUM-CAPRICORNUTUM; COPPER TOLERANCE; TOXICITY; GROWTH; WATER; CADMIUM; PHYTOPLANKTON	The effect of zinc acclimation of Raphidocelis subcapitata (syn. Selenastrum capricornutum) and Chlorella vulgaris on their sensitivity towards this metal was examined in a series of laboratory experiments. These two commonly used algal species were acclimated to 65 mug Zn/l and changes in zinc tolerance were monitored using standard growth inhibition tests. The chemically defined ISO medium was used as a control culture medium. Both species demonstrated a maximum increase in zinc tolerance of a factor of 3 after 100 days of acclimation. Shifts in the shape of the concentration-response curve due to acclimation were observed for R subcapitata. Compared to non-acclimated algae, acclimated R. subcapitata exhibited higher growth rates in all zinc treatments as well as in the controls. This suggests that the use of ISO-medium results in sub-optimal growth due to zinc deficiency. These effects could not be demonstrated for C vulgaris. The zinc tolerance of both species decreased significantly one week after returning the acclimated algae to control (ISO) medium. 72hEC50 values based on growth rate were two to four times higher than those calculated using biomass measurements. Algal toxicity test results, particularly if used for metal risk assessments, must not be conducted using nutrient deficient media. (C) 2001 Elsevier Science Ltd. All rights reserved.	State Univ Ghent, Lab Environm Toxicol & Aquat Ecol, B-9000 Ghent, Belgium	Muyssen, BTA (corresponding author), State Univ Ghent, Lab Environm Toxicol & Aquat Ecol, J Plateaustr 22, B-9000 Ghent, Belgium.		Janssen, Colin R./H-3122-2015	Janssen, Colin/0000-0002-7781-6679			Allison J.D., 1991, MINTEQA2 PRODEFA2 GE, P106; ANDERSON MA, 1978, NATURE, V276, P70, DOI 10.1038/276070a0; BARTLETT L, 1974, WATER RES, V8, P179, DOI 10.1016/0043-1354(74)90041-4; Blaise C, 1986, TOXIC ASSESS, V1, P261, DOI DOI 10.1002/T0X.2540010302; Campbell CD, 2000, CHEMOSPHERE, V40, P319, DOI 10.1016/S0045-6535(99)00302-1; Chen CY, 1997, ENVIRON TOXICOL CHEM, V16, P1337, DOI [10.1897/1551-5028(1997)016&lt;1337:OAPEOT&gt;2.3.CO;2, 10.1002/etc.5620160701]; Chu SP, 1942, J ECOL, V30, P284, DOI 10.2307/2256574; COLEMAN RD, 1971, BOT GAZ, V132, P102, DOI 10.1086/336568; Errecalde O, 1998, WATER RES, V32, P419, DOI 10.1016/S0043-1354(97)00282-0; FOSTER PL, 1977, NATURE, V269, P322, DOI 10.1038/269322a0; FOSTER PL, 1982, FRESHWATER BIOL, V12, P41, DOI 10.1111/j.1365-2427.1982.tb00602.x; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; *ISO, 1987, 8692 ISODIS, P1; JENSEN A, 1974, J EXP MAR BIOL ECOL, V15, P145, DOI 10.1016/0022-0981(74)90040-9; KLAINE S J, 1983, Environmental Toxicology and Chemistry, V2, P245, DOI 10.1897/1552-8618(1983)2[245:GOABFT]2.0.CO;2; KLERKS PL, 1987, ENVIRON POLLUT, V45, P173, DOI 10.1016/0269-7491(87)90057-1; Knauer K, 1997, ENVIRON TOXICOL CHEM, V16, P220, DOI 10.1002/etc.5620160218; KUWABARA JS, 1986, ENVIRON TOXICOL CHEM, V5, P197, DOI 10.1002/etc.5620050211; KUWABARA JS, 1985, ENVIRON SCI TECHNOL, V19, P417, DOI 10.1021/es00135a005; MAEDA S, 1990, CHEMOSPHERE, V21, P953, DOI 10.1016/0045-6535(90)90118-D; Miller W.E., 1978, EPA600978018; MILLINGTON LA, 1988, WATER RES, V22, P1593, DOI 10.1016/0043-1354(88)90173-X; Morel F. M. M., 1975, 16 MIT DEP DIV ENG L, P1; MUYSSEN BTA, 2001, IN PRESS ENV TOXICOL; NICHOLS HW, 1965, J PHYCOL, V1, P34, DOI 10.1111/j.1529-8817.1965.tb04552.x; NYHOLM N, 1985, WATER RES, V19, P273, DOI 10.1016/0043-1354(85)90085-5; OECD, 1984, OECD GUID TEST CHEM, V1, P16, DOI 10.1787/9789264070042-en; PRICE NM, 1994, ERGEB LIMNOL, V42, P79; RACHLIN JW, 1974, WATER RES, V8, P575, DOI 10.1016/0043-1354(74)90066-9; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Sillanpaa M, 1996, CHEMOSPHERE, V32, P1485, DOI 10.1016/0045-6535(96)00057-4; SRIVASTAVA M, 1991, POLLUT RES, V10, P49; STAUBER JL, 1989, WATER RES, V23, P907, DOI 10.1016/0043-1354(89)90016-X; Stein J.R., 1973, HDB PHYCOLOGICAL MET; STOKES PM, 1981, CAN J BOT, V59, P1817, DOI 10.1139/b81-242; STOKES PM, 1973, CAN J BOT, V51, P2155, DOI 10.1139/b73-278; TOUSSAINT MW, 1995, ENVIRON TOXICOL CHEM, V14, P907, DOI [10.1897/1552-8618(1995)14[907:ACOSAT]2.0.CO;2, 10.1002/etc.5620140524]; TURBAK SC, 1986, WATER RES, V20, P91, DOI 10.1016/0043-1354(86)90219-8; VANEWIJK PH, 1993, ECOTOX ENVIRON SAFE, V25, P25, DOI 10.1006/eesa.1993.1003; VASSEUR P, 1988, TOXIC ASSESS, V3, P331, DOI 10.1002/tox.2540030308; WANG WC, 1986, WATER AIR SOIL POLL, V28, P335; Whitton B. A., 1984, Algae as ecological indicators, P257; WREN MJ, 1990, ENVIRON POLLUT, V64, P87, DOI 10.1016/0269-7491(90)90097-V	43	50	56	3	31	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND	0045-6535			CHEMOSPHERE	Chemosphere	NOV	2001	45	4-5					507	514		10.1016/S0045-6535(01)00047-9			8	Environmental Sciences	Environmental Sciences & Ecology	480FT	WOS:000171451900012	11680746				2021-04-07	
J	Delaroque, N; Muller, DG; Bothe, G; Pohl, T; Knippers, R; Boland, W				Delaroque, N; Muller, DG; Bothe, G; Pohl, T; Knippers, R; Boland, W			The complete DNA sequence of the Ectocarpus siliculosus virus EsV-1 genome	VIROLOGY			English	Article						Phycodnaviridae; Ectocarpus siliculosus; histidine kinase; phytochrome; integration; transposase; polysaccharide metabolism	BROWN ALGAL VIRUS; PROTEIN SEQUENCES; POLYMERASE GENE; PHAEOPHYCEAE; CONTAINS; PBCV-1; FAMILY; PREDICTION; DOMAIN; MOTIF	The Ectocarpus siliculosus Virus-1, EsV-1, is the type-species of a genus of Phycodnaviridae, the phaeoviruses, infecting marine filamentous brown algae. The EsV-1 genome of 335,593 by contains tandem and dispersed repetitive elements in addition to a large number of open reading frames of which 231 are currently counted as genes. Many genes can be assigned to functional groups involved in DNA synthesis, DNA integration, transposition, and polysaccharide metabolism. Furthermore, EsV-1 contains components of a surprisingly complex signal transduction system with six different hybrid histidine protein kinases and four putative serine/threonine protein kinases. Several other genes encode polypeptides with protein-protein interaction domains. However, 50% of the predicted genes have no counterparts in data banks. Only 28 of the 231 identified genes have significant sequence similarities to genes of the Chlorella virus PBCV-1, another phycodnavirus. To our knowledge, the EsV-1 genome is the largest viral DIVA sequenced to date. (C) 2001 Academic Press.	Univ Konstanz, Dept Biol, D-78457 Constance, Germany; GATC Biotech AG, D-78467 Constance, Germany; Max Planck Inst Chem Oekol, D-07745 Jena, Germany	Delaroque, N (corresponding author), Univ Konstanz, Dept Biol, D-78457 Constance, Germany.		Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; BARDWELL VJ, 1994, GENE DEV, V8, P1664, DOI 10.1101/gad.8.14.1664; Bateman A, 2000, NUCLEIC ACIDS RES, V28, P263, DOI 10.1093/nar/28.1.263; Bawden AL, 2000, VIROLOGY, V274, P120, DOI 10.1006/viro.2000.0449; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; BRENDEL V, 1992, P NATL ACAD SCI USA, V89, P2002, DOI 10.1073/pnas.89.6.2002; Cairns BR, 1996, GENE DEV, V10, P2131, DOI 10.1101/gad.10.17.2131; Davis SJ, 1999, SCIENCE, V286, P2517, DOI 10.1126/science.286.5449.2517; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2000, VIROLOGY, V269, P148, DOI 10.1006/viro.2000.0225; Delaroque N, 2000, VIROLOGY, V273, P383, DOI 10.1006/viro.2000.0383; FASMAN GD, 1990, TRENDS BIOCHEM SCI, V15, P89, DOI 10.1016/0968-0004(90)90187-G; GRABHERR R, 1992, VIROLOGY, V188, P721, DOI 10.1016/0042-6822(92)90527-V; Grebe TW, 1999, ADV MICROB PHYSIOL, V41, P139, DOI 10.1016/S0065-2911(08)60167-8; HENRY EC, 1994, J APPL PHYCOL, V6, P247, DOI 10.1007/BF02186078; Hoch J.A., 1995, 2 COMPONENT SIGNAL T; Hofmann K, 1999, NUCLEIC ACIDS RES, V27, P215, DOI 10.1093/nar/27.1.215; Hu X, 1997, PLANT MOL BIOL, V34, P949, DOI 10.1023/A:1005893119263; Hubscher U, 2000, TRENDS BIOCHEM SCI, V25, P143, DOI 10.1016/S0968-0004(99)01523-6; JONES RS, 1993, MOL CELL BIOL, V13, P6357, DOI 10.1128/MCB.13.10.6357; Kelley WL, 1998, TRENDS BIOCHEM SCI, V23, P222, DOI 10.1016/S0968-0004(98)01215-8; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; Kotani H, 1998, ANNU REV PLANT PHYS, V49, P151, DOI 10.1146/annurev.arplant.49.1.151; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; Kutish GF, 1996, VIROLOGY, V223, P303, DOI 10.1006/viro.1996.0482; Kuzio J, 1999, VIROLOGY, V253, P17, DOI 10.1006/viro.1998.9469; LANDY A, 1993, CURR OPIN GENET DEV, V3, P699, DOI 10.1016/S0959-437X(05)80086-3; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; Lavrukhin OV, 2000, J BIOL CHEM, V275, P6915, DOI 10.1074/jbc.275.10.6915; Lee AM, 1998, J PHYCOL, V34, P608, DOI 10.1046/j.1529-8817.1998.340608.x; Lee AM, 1998, VIROLOGY, V248, P35, DOI 10.1006/viro.1998.9245; LUPAS A, 1991, SCIENCE, V252, P1162, DOI 10.1126/science.252.5009.1162; Maier I, 1998, EUR J PHYCOL, V33, P213, DOI 10.1017/S0967026298001747; Miyata S, 1998, FEBS LETT, V437, P11, DOI 10.1016/S0014-5793(98)01188-0; Mizuno T, 1997, DNA Res, V4, P161, DOI 10.1093/dnares/4.2.161; Mossi R, 1998, EUR J BIOCHEM, V254, P209, DOI 10.1046/j.1432-1327.1998.2540209.x; Muller DG, 1996, PHYCOLOGIA, V35, P61, DOI 10.2216/i0031-8884-35-1-61.1; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Muller DG, 2000, BOT MAR, V43, P157, DOI 10.1515/BOT.2000.016; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; MYERS RS, 1994, ANNU REV GENET, V28, P49, DOI 10.1146/annurev.ge.28.120194.000405; Nielsen H, 1997, PROTEIN ENG, V10, P1, DOI 10.1093/protein/10.1.1; Nunes-Duby SE, 1998, NUCLEIC ACIDS RES, V26, P391, DOI 10.1093/nar/26.2.391; Plugge B, 2000, SCIENCE, V287, P1641, DOI 10.1126/science.287.5458.1641; POHL TM, 1995, BIOTECHNIQUES, V19, P482; Pringle C R, 1998, Arch Virol, V143, P1449, DOI 10.1007/s007050050389; REZSOHAZY R, 1993, MOL MICROBIOL, V9, P1283, DOI 10.1111/j.1365-2958.1993.tb01258.x; Sambrook J., 1989, MOL CLONING LAB MANU; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; Schultz J, 2000, NUCLEIC ACIDS RES, V28, P231, DOI 10.1093/nar/28.1.231; SCHUSTER AM, 1990, VIROLOGY, V176, P515, DOI 10.1016/0042-6822(90)90021-I; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; STASSEN MJ, 1995, MECH DEVELOP, V52, P209, DOI 10.1016/0925-4773(95)00402-M; Stock AM, 2000, ANNU REV BIOCHEM, V69, P183, DOI 10.1146/annurev.biochem.69.1.183; Suzuki T, 1998, PLANT CELL PHYSIOL, V39, P1258, DOI 10.1093/oxfordjournals.pcp.a029329; TSCHIERSCH B, 1994, EMBO J, V13, P3822, DOI 10.1002/j.1460-2075.1994.tb06693.x; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; Villarreal LP, 2000, J VIROL, V74, P7079, DOI 10.1128/JVI.74.15.7079-7084.2000; VONHEIJNE G, 1992, J MOL BIOL, V225, P487, DOI 10.1016/0022-2836(92)90934-C; Wolf S, 2000, EUR J PHYCOL, V35, P163, DOI 10.1017/S0967026200002663; Wolf' S, 1998, PROTOPLASMA, V203, P153, DOI 10.1007/BF01279472; YANEZ RJ, 1995, VIROLOGY, V208, P249, DOI [10.1006/viro.1995.1149, 10.1006/viro.1994.1616]; Yeh KC, 1997, SCIENCE, V277, P1505, DOI 10.1126/science.277.5331.1505	66	90	95	0	13	ACADEMIC PRESS INC	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA	0042-6822			VIROLOGY	Virology	AUG 15	2001	287	1					112	132		10.1006/viro.2001.1028			21	Virology	Virology	465TT	WOS:000170606600013	11504547				2021-04-07	
J	Schmid, R; Hillrichs, S				Schmid, R; Hillrichs, S			Uptake and accumulation of inorganic carbon in Ectocarpus siliculosus and its relation to blue light stimulation of photosynthesis	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						blue light; carbon acquisition; carbon pool; Ectocarpus; Phaeophyceae; photosynthesis; uptake	BROWN-ALGAE; SATURATED PHOTOSYNTHESIS; FAST RESPONSES; CIRCADIAN RHYTHMICITY; ACQUISITION; CO2; BICARBONATE; PHAEOPHYTA; LAMINARIA; ANHYDRASE	The stimulating effect of blue light on the carbon-concentrating mechanism of Ectocarpus siliculosus was investigated by measuring the intracellular pools of inorganic carbon (Ci) in red light (low photosynthetic rates) and in red light plus blue light (high photosynthetic rates). Ci accumulated in red light to about twice the levels found in the presence of blue light, but the permeability of the cell membranes for Ci, measured as the rate of influx of tracer carbon, was similar in both light conditions. This was also true after the seawater pH was adjusted to pH 9.5, when bicarbonate should be the only inorganic carbon species to enter the cells. Activation of photosynthesis after prolonged red light caused the accumulated Ci to decline to levels similar to those observed in blue light. The time course of this decline was rapid and coincided with the time course that was expected for the emptying of a carbon pool whose existence had been demonstrated earlier in measurements of photosynthetic oxygen evolution in the absence of external carbon. The accumulation of Ci in red light required active photosynthesis, but it also occurred in the presence of inhibitors of carbonic anhydrase activity. It is suggested that blue light mobilizes the Ci pool by causing changes in intracellular pH and that the size of the pool depends on the activity of metabolic CO(2)-sink activities that are also under the control of blue light.	Univ Marburg, Fachbereich Biol Bot, D-35032 Marburg, Germany	Schmid, R (corresponding author), Univ Marburg, Fachbereich Biol Bot, D-35032 Marburg, Germany.	schmid@maiter.uni-marburg.de					Axelsson L., 1991, SEAWEED CELLULAR BIO, P185; Badger MR, 1998, CAN J BOT, V76, P1052, DOI 10.1139/b98-074; Beer S, 1994, PROGR PHYCOLOGICAL R, V10, P179; Busch S, 2001, EUR J PHYCOL, V36, P61, DOI 10.1080/09670260110001735208; DRING MJ, 1994, MAR ECOL PROG SER, V113, P271, DOI 10.3354/meps113271; DROMGOOLE FI, 1987, BOT MAR, V30, P331, DOI 10.1515/botm.1987.30.4.331; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; GIMMLER H, 1990, J EXP BOT, V41, P785, DOI 10.1093/jxb/41.7.785; GUTKNECHT J, 1977, J GEN PHYSIOL, V69, P779; Hillrichs S, 2001, EUR J PHYCOL, V36, P71, DOI 10.1017/S096702620100302X; JOHNSTON AM, 1991, CAN J BOT, V69, P1123, DOI 10.1139/b91-144; JOSHI G V, 1973, Proceedings of the Indian National Science Academy Part B Biological Sciences, V39, P489; Keeley JE, 1996, ECOL STU AN, V114, P281; KERBY NW, 1985, ADV BOT RES, V11, P71, DOI 10.1016/S0065-2296(08)60169-X; MORONEY JV, 1985, PLANT PHYSIOL, V79, P177, DOI 10.1104/pp.79.1.177; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; Provasoli L., 1968, CULTURES COLLECTIONS, P63; Raven JA, 1997, ADV BOT RES, V27, P85; Raven JA, 1997, EUR J PHYCOL, V32, P319, DOI 10.1080/09670269710001737259; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; Schmid R, 1998, PLANT CELL ENVIRON, V21, P523, DOI 10.1046/j.1365-3040.1998.00297.x; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Schmid R, 1996, PLANT CELL ENVIRON, V19, P383, DOI 10.1111/j.1365-3040.1996.tb00330.x; Schmid R, 1996, SCI MAR, V60, P115; SULTEMEYER D, 1996, PLANTA, V200, P258	28	6	6	0	9	TAYLOR & FRANCIS LTD	ABINGDON	4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	AUG	2001	36	3					257	264		10.1017/S0967026201003274			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	471EY	WOS:000170916400006					2021-04-07	
J	Lluch, JR; Garreta, AG				Lluch, JR; Garreta, AG			Contribution to the seaweed flora of Namibia: new records of Chlorophyta and Phaeophyceae	CRYPTOGAMIE ALGOLOGIE			English	Article						benthic marine algae; Namibia; Chlorophyta; Phaeophyceae	SOUTH-AFRICA; SP-NOV; MEDITERRANEAN SEAWEEDS; COMB-NOV; RHODOPHYTA; CHECKLIST; CORALLINACEAE; ECTOCARPALES; ALGAE	Six species of benthic marine Chlorophyta and Phaeophyceae are recorded for the first time from the coast of Namibia: Ectocarpus fasciculatus Harvey, Feldmannia irregularis (Kutzing) G. Hamel, Bachelotia antillarum (Grunow) Gerloff, Stragularia clavata (Harvey in Hooker) Hamel, Entocladia leptochaete (Huber) Burrows and Ulvella lens P. Crouan & H. Crouan. Data concerning local and world distribution of these species as well as some taxonomical remarks are provided. (C) 2001 Adac/Editions scientifiques et medicales Elsevier SAS.	Univ Barcelona, Fac Farm, Lab Bot, E-08028 Barcelona, Spain	Lluch, JR (corresponding author), Univ Barcelona, Fac Farm, Lab Bot, Avda Joan 23 S-N, E-08028 Barcelona, Spain.						ANDERSON RJ, 1990, BRIT PHYCOL J, V25, P381, DOI 10.1080/00071619000650431; Ardre F., 1970, PORT ACTA BIOL B, V10, P1; Baardseth E., 1941, RESULTS NORWEGIAN SC, V9, P1; BLOMQUIST H. L., 1958, JOUR ELISHA MITCHELL SCI SOC, V74, P25; BURROWS EM, 1991, SEAWEEDS BRIT ISLES; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; Chamberlain YM, 1996, PHYCOLOGIA, V35, P204, DOI 10.2216/i0031-8884-35-3-204.1; CHAMBERLAIN YM, 1994, PHYCOLOGIA, V33, P111, DOI 10.2216/i0031-8884-33-2-111.1; CHAMBERLAIN YM, 1994, PHYCOLOGIA, V33, P141, DOI 10.2216/i0031-8884-33-3-141.1; CHAMBERLAIN YM, 1995, S AFR J BOT, V61, P134, DOI 10.1016/S0254-6299(15)30499-3; DANGEARD PIERRE, 1931, BULL SOC BOT FRANCE, V78, P312; DELEPINE R, 1976, Phycologia, V15, P425, DOI 10.2216/i0031-8884-15-3-425.1; DELEPINE R, 1975, B SOC BOT FR, V122, P295, DOI 10.1080/00378941.1975.10835618; DEREVIERS B, 1999, PROGR PHYCOLOGICAL R, V13, P107; Earle SA, 1969, PHYCOLOGIA, V7, P71, DOI 10.2216/i0031-8884-7-2-71.1; EDELSTEIN T, 1970, CAN J BOTANY, V48, P527, DOI 10.1139/b70-074; ENGLEDOW HR, 1998, THESIS U CAPE TOWN; Fletcher R.L., 1987, SEAWEEDS BRIT ISLE 1; GALLARDO T, 1993, BOT MAR, V36, P399, DOI 10.1515/botm.1993.36.5.399; GAYRAL P, 1966, ALGUES COTES FRANCAI; HAMEL G, 1939, PHEOPHYCEES FRANCE, P337; HAMEL G., 1937, PHEOPHYCEES FRANCE, P177; HAMEL G., 1935, PHEOPHYCEES FRANCE, P81; HAMEL G, 1938, PHEOPHYCEES FRANCE, P241; Hamel G, 1931, PHEOPHYCEES FRANCE, P1; Hooker WJ, 1833, ENGLISH FLORA SIR 1, V5, P1; JOHN D M, 1974, British Phycological Journal, V9, P285; Keats DW, 1997, PHYCOLOGIA, V36, P447, DOI 10.2216/i0031-8884-36-6-447.1; KEATS DW, 1995, PHYCOLOGIA, V34, P51, DOI 10.2216/i0031-8884-34-1-51.1; LAWSON G.W., 1995, B NAT HIST MUS LOND, V25, P99; LAWSON GW, 1975, BOT J LINN SOC, V70, P307, DOI 10.1111/j.1095-8339.1975.tb01652.x; Lawson GW, 1990, B BR MUS NAT HIS B, V20, P153; LAWSON GW, 1987, BEIH NOVA HEDWIGIA, V93, P1; LEVRING TORE, 1938, LANDS UNIV ARSSKR AVD 2, V34, P1; LLUCH JR, 1993, NOVA HEDWIGIA, V56, P465; LLUCH JR, 1999, THESIS U BARCELONA; MOLLOY FJ, 1990, HYDROBIOLOGIA, V204, P293, DOI 10.1007/BF00040248; MOLLOY FJ, 1995, J APPL PHYCOL, V7, P381, DOI 10.1007/BF00003795; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; Newton L, 1931, HDB BRIT SEAWEEDS; NIELSEN R, 1977, BRIT PHYCOL J, V12, P1, DOI 10.1080/00071617700650011; NODA M, 1987, MARINE ALGAE JAPAN S; PEDRINI AG, 1989, BOT MAR, V32, P97, DOI 10.1515/botm.1989.32.2.97; PRICE J.H., 1978, B BR MUS NAT HIS B, V6, P87; RIBERA MA, 1992, BOT MAR, V35, P109, DOI 10.1515/botm.1992.35.2.109; Rousseau F, 2001, CR ACAD SCI III-VIE, V324, P305, DOI 10.1016/S0764-4469(01)01306-3; SCHNETTER R., 1978, BIBLIOTHECA PHYCOLOG, V42, P1; Seagrief SC, 1984, MEM BOT SURVEY S AFR, V47, P1; Setchell W. A., 1924, P CALIF ACAD SCI, V4, P695; Setchell WA, 1925, U CALIF PUBL BOT, V8, P383; SHANNON LV, 1985, OCEANOGR MAR BIOL, V23, P105; Silva P.C., 1996, CATALOGUE BENTHIC MA; South G. R., 1986, CHECKLIST DISTRIBUTI; SOUTH GR, 1984, CAN J BOT, V62, P680, DOI 10.1139/b84-102; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Stegenga H., 1997, CONTRIBUTIONS BOLUS, V18, P1; STEGENGA H, 1997, GORTERIA, V4, P1; Taylor WR, 1957, MARINE ALGAE NE COAS; Taylor WR, 1960, MARINE ALGAE E TROPI; Womersley H.B.S., 1987, MARINE BENTHIC FLO 2; WOMERSLEY HB, 1967, AUST J BOT, V15, P189, DOI 10.1071/BT9670189; Wynne M.J., 1998, NOVA HEDWIGIA S, V116, P1; Yoneshigue Y, 1985, THESIS U AIX MARSEIL	63	2	2	0	4	EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER	PARIS CEDEX 15	23 RUE LINOIS, 75724 PARIS CEDEX 15, FRANCE	0181-1568			CRYPTOGAMIE ALGOL	Cryptogam. Algol.	JUL-SEP	2001	22	3					297	304					8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	482RN	WOS:000171589200005					2021-04-07	
J	Iken, K; Amsler, CD; Greer, SR; McClintock, JB				Iken, K; Amsler, CD; Greer, SR; McClintock, JB			Qualitative and quantitative studies of the swimming behaviour of Hincksia irregularis (Phaeophyceae) spores: ecological implications and parameters for quantitative swimming assays	PHYCOLOGIA			English	Article							KELPS MACROCYSTIS-PYRIFERA; PTERYGOPHORA-CALIFORNICA; ECTOCARPUS-SILICULOSUS; NEUTRAL LIPIDS; BROWN ALGA; SETTLEMENT; DISPERSAL; PROPAGULES; RECRUITMENT; ATTACHMENT	The capacity for motility is important for many micro-organisms, and also for macroalgal spores, because it can allow them to explore a physically and chemically heterogeneous environment and react to the perceived information by movement towards favourable conditions or away from hazardous conditions. The swimming behaviour of spores of the brown alga Hincksia irregularis was analysed using computer-assisted motion analysis. We distinguished five main swimming patterns: straight paths, search circles, orientation, gyration and wobbling. Different functions can be suggested for different swimming patterns in the context of spore settlement. Straight paths may be important in small-scale movements in the benthic boundary layer to locate suitable microenvironments. Gradients in chemical or physical parameters may be detected during search circles. and orientation should facilitate the detection of the structure of surfaces. Gyration occurs during the initial reversible phase of spore adhesion, which can ultimately lead to settlement. Wobbling patterns do not appear to be associated with settlement and may typify irritated or mechanically damaged spores. The dominant swimming pattern changed with spore age (in the period from 10 +/- 5 to 60 +/- 5 min of spore age), with younger spores swimming primarily in straight lines and search circles. whereas older spores swam in orientation and gyration patterns. These changes in swimming patterns can be quantified using speed (SPD), which decreases over time, and the rate of change of direction (RCD), which increases over time. We suggest that computer-assisted motion analysis is an efficient way to bioassay the influence of environmental factors on spore movements. Reaction to environmental factors can be quantified as changes in SPD and RCD of spore swimming.	Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA	Iken, K (corresponding author), Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA.	iken@uab.edu		Amsler, Charles/0000-0002-4843-3759			Alberte RS, 1992, BIOFOULING, V6, P91, DOI 10.1080/08927019209386214; AMSLER CD, 1993, J BACTERIOL, V175, P6238, DOI 10.1128/JB.175.19.6238-6244.1993; Amsler CD, 1996, ANAL BIOCHEM, V235, P20, DOI 10.1006/abio.1996.0086; AMSLER CD, 1990, MAR BIOL, V107, P297, DOI 10.1007/BF01319829; AMSLER CD, 1991, J PHYCOL, V27, P26, DOI 10.1111/j.0022-3646.1991.00026.x; AMSLER CD, 1992, BRIT PHYCOL J, V27, P253, DOI 10.1080/00071619200650251; AMSLER CD, 1980, J PHYCOL, V16, P617, DOI 10.1111/j.1529-8817.1980.tb03080.x; AMSLER CD, 1989, MAR BIOL, V102, P557, DOI 10.1007/BF00438358; Amsler CD, 1999, J PHYCOL, V35, P239, DOI 10.1046/j.1529-8817.1999.3520239.x; AMSLER CD, 2001, MARINE CHEM ECOLOGY, P373; AMSLER CD, 1983, THESIS U N CAROLINA; BEACH KS, 1995, MAR ECOL PROG SER, V125, P229, DOI 10.3354/meps125229; BRAWLEY SH, 1991, J PHYCOL, V27, P179, DOI 10.1111/j.0022-3646.1991.00179.x; BROWN D A, 1974, Proceedings of the National Academy of Sciences of the United States of America, V71, P1388, DOI 10.1073/pnas.71.4.1388; Bryan PJ, 1996, J EXP MAR BIOL ECOL, V196, P79, DOI 10.1016/0022-0981(95)00124-7; BRZEZINSKI MA, 1993, J PHYCOL, V29, P16, DOI 10.1111/j.1529-8817.1993.tb00275.x; Callow ME, 1997, J PHYCOL, V33, P938, DOI 10.1111/j.0022-3646.1997.00938.x; CHAMBERLAIN A. H. L., 1975, MICROBIAL ASPECTS DE, P59; CHAMBERLAIN AHL, 1976, P 3 INT BIOD S, P417; CHRISTIE AO, 1973, P 3 INT C MAR CORR F, P674; CLAYTON MN, 1992, BRIT PHYCOL J, V27, P219, DOI 10.1080/00071619200650231; Crisp D.J., 1984, P103; DILLON PS, 1989, MICROBIAL ECOL, V17, P39, DOI 10.1007/BF02025592; Dusenbery DB, 1998, BIOPHYS J, V74, P2272, DOI 10.1016/S0006-3495(98)77936-6; Dusenbery DB, 1997, P NATL ACAD SCI USA, V94, P10949, DOI 10.1073/pnas.94.20.10949; Fletcher R. L, 1984, MARINE BIODETERIORAT, P172; FLETCHER RL, 1992, BRIT PHYCOL J, V27, P303, DOI 10.1080/00071619200650281; FREDRIKSEN S, 1995, SARSIA, V80, P47, DOI 10.1080/00364827.1995.10413579; Haderlie E.C, 1984, MARINE BIODETERIORAT, P163, DOI DOI 10.1007/978-1-4615-9720-9_21; HARLIN MM, 1977, MAR BIOL, V40, P33, DOI 10.1007/BF00390625; HARRISON PJ, 1980, J PHYCOL, V16, P28, DOI 10.1111/j.1529-8817.1980.tb00724.x; JONES W E, 1968, British Phycological Bulletin, V3, P525; KAWAI H, 1991, PROTOPLASMA, V161, P17, DOI 10.1007/BF01328893; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; Luchsinger RH, 1999, BIOPHYS J, V77, P2377, DOI 10.1016/S0006-3495(99)77075-X; MACNAB RM, 1972, P NATL ACAD SCI USA, V69, P2509, DOI 10.1073/pnas.69.9.2509; MAIER I, 1992, J EXP BOT, V43, P1651, DOI 10.1093/jxb/43.12.1651; MAKI JS, 1989, BIOL BULL, V177, P295, DOI 10.2307/1541944; MCLACHLAN J, 1978, CAN J BOT, V56, P371, DOI 10.1139/b78-045; MULLER DG, 1965, NATURWISSENSCHAFTEN, V52, P311; MULLER DIETER G., 1964, Z BOT, V52, P193; NEUSHUL M, 1976, J PHYCOL, V12, P397, DOI 10.1111/j.0022-3646.1976.00397.x; NORTON TA, 1992, BRIT PHYCOL J, V27, P293, DOI 10.1080/00071619200650271; NORTON TA, 1981, J MAR BIOL ASSOC UK, V61, P920; Provasoli L., 1968, CULTURES COLLECTIONS, P63; REED DC, 1992, ECOLOGY, V73, P1577, DOI 10.2307/1940011; REED DC, 1988, ECOL MONOGR, V58, P321, DOI 10.2307/1942543; Reed DC, 1999, MAR BIOL, V133, P737, DOI 10.1007/s002270050515; SANTELICES B, 1990, OCEANOGR MAR BIOL, V28, P177; SHEA C, 1990, BIOTECHNIQUES, V8, P610; SHEA C, 1995, J IND MICROBIOL, V15, P290, DOI 10.1007/BF01569982; Sokal R.R., 1995, BIOMETRY, V3; WAHL M, 1989, MAR ECOL PROG SER, V58, P175, DOI 10.3354/meps058175; WATANUKI A, 1990, HYDROBIOLOGIA, V204, P275, DOI 10.1007/BF00040245; ZECHMAN FW, 1985, BOT MAR, V28, P283, DOI 10.1515/botm.1985.28.7.283	55	24	25	0	5	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	JUL	2001	40	4					359	366		10.2216/i0031-8884-40-4-359.1			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	471HG	WOS:000170922300006					2021-04-07	
J	Peters, AF; Ramirez, ME				Peters, AF; Ramirez, ME			Molecular phylogeny of small brown algae, with special reference to the systematic position of Caepidium antarcticum (Adenocystaceae, Ectocarpales)	CRYPTOGAMIE ALGOLOGIE			English	Article						Acinetosporaceae; Adenocystis utricularis; brown algae; Caepidium antarcticum; Chordariaceae; Geminocarpus austro-georgiae; molecular phylogeny; nuclear ribosomal DNA; Phaeophyceae; rbcL; Rubisco spacer; taxonomy; Utriculidium durvillei	DNA-SEQUENCES; ORDER ECTOCARPALES; SPACER SEQUENCES; PHAEOPHYCEAE; DICTYOSIPHONALES; NOV.	Nuclear ribosomal DNA (3'-SSU, ITS, 5'-LSU) and plastid-encoded (rbcL and Rubisco spacer) sequences were determined in Caepidium antarcticum and compared to homologous sequences of relatives from Ectocarpales, Scytothamnales, and other brown algae. Plastidial sequences confirmed a previous conclusion from nuclear ribosomal sequences that some taxa with stellate plastids (Asterocladon and relatives) form the closest outgroup to the Ectocarpales as yet identified. To reconcile nomenclature with the clades resolved in recent molecular studies, we propose a subdivision of the Ectocarpales in five families. Plastidial sequences support the recent proposal of Adenocystaceae, and all sequences suggest that Caepidium should be included in this family. As a further result, Geminocarpus was shown to belong to the same clade as Pylaiella and a number of other brown algae with an isomorphic life history and discoid plastids. We recognise this clade, whose correct name is Acinetosporaceae, as another family in the Ectocarpales. We also propose to unite a number of genetically related taxa, which were formerly classified in different families, in an extended Chordariaceae. The remaining species of the Ectocarpales belong to Scytosiphonaceae and to Ectocarpaceae, the latter containing only Ectocarpus and Kuckuckia. (C) 2001 Adac/Editions scientifiques et medicales Elsevier SAS.	Inst Meereskunde, D-24105 Kiel, Germany	Peters, AF (corresponding author), Inst Meereskunde, Dusternbrooker Weg 20, D-24105 Kiel, Germany.						Agardh C. A., 1828, SPECIES ALGARUM RITE, p[i]; BAKKER FT, 1995, EUR J PHYCOL, V30, P197, DOI 10.1080/09670269500650981; DEREVIERS B, 1999, PROGR PHYCOLOGICAL R, V13, P107; FARLOW WG, 1881, MARINE ALGAE NEW ENG, P1; FELDMANN J, 1937, REV ALGOL, V9, P141; GREVILLE RK, 1830, ALGAE BRITANNICAE; Kogame K, 1999, PHYCOLOGIA, V38, P496, DOI 10.2216/i0031-8884-38-6-496.1; KYLIN H, 1940, ACTA U LUND, V36, P1; Muller DG, 1998, PHYCOLOGIA, V37, P425, DOI 10.2216/i0031-8884-37-6-425.1; MULLER DG, 1969, NATURWISSENSCHAFTEN, V56, P220; MULLER DG, 1984, PHYCOLOGIA, V23, P87, DOI 10.2216/i0031-8884-23-1-87.1; NELSON WA, 1983, NEW ZEAL J BOT, V21, P77; NELSON WA, 1982, BOT MAR, V25, P371, DOI 10.1515/botm.1982.25.8.371; PEDERSEN P.M., 1984, OPERA BOT, V74, P1; PEDERSEN PM, 1978, PHYCOLOGIA, V17, P61, DOI 10.2216/i0031-8884-17-1-61.1; Peters AF, 1997, J PHYCOL, V33, P294, DOI 10.1111/j.0022-3646.1997.00294.x; Peters AF, 1998, PHYCOLOGIA, V37, P114, DOI 10.2216/i0031-8884-37-2-114.1; Peters AF, 1998, PHYCOLOGIA, V37, P106, DOI 10.2216/i0031-8884-37-2-106.1; REINCKE T, 1998, THESIS U KIEL; Ricker RW, 1987, TAXONOMY BIOGEOGRAPH; Rousseau F, 1999, CRYPTOGAMIE ALGOL, V20, P5, DOI 10.1016/S0181-1568(99)80002-6; Rousseau F, 2000, EUR J PHYCOL, V35, P35, DOI 10.1017/S0967026200002638; Setchell WA, 1925, U CALIF PUBL BOT, V8, P383; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; SIEMER BL, 1998, THESIS U COPENHAGEN; Silva P. C., 1980, REGNUM VEG, V103, P1; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; SWOFFORD DL, 2000, PAUP PHYLOGENETIC AN; TAN IH, 1994, J PHYCOL, V30, P721, DOI 10.1111/j.0022-3646.1994.00721.x; van den Hoek C, 1995, ALGAE INTRO PHYCOLOG	30	47	52	0	11	EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER	PARIS CEDEX 15	23 RUE LINOIS, 75724 PARIS CEDEX 15, FRANCE	0181-1568			CRYPTOGAMIE ALGOL	Cryptogam. Algol.	APR-JUN	2001	22	2					187	200		10.1016/S0181-1568(01)01062-5			14	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	445FK	WOS:000169447300003					2021-04-07	
J	Busch, S; Schmid, R				Busch, S; Schmid, R			Enzymes associated with beta-carboxylation in Ectocarpus siliculosus (Phaeophyceae): Are they involved in net carbon acquisition?	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						blue light; carbon acquisition; circadian rhythm; Ectocarpus; malic enzyme; phosphoenolpyruvate carboxykinase; photosynthesis	LIGHT-SATURATED PHOTOSYNTHESIS; ASCOPHYLLUM-NODOSUM PHAEOPHYCEAE; CRASSULACEAN ACID METABOLISM; DEPENDENT MALIC ENZYME; MESEMBRYANTHEMUM-CRYSTALLINUM L; BLUE-LIGHT; PHOSPHOENOLPYRUVATE CARBOXYKINASE; BROWN-ALGAE; INORGANIC CARBON; CIRCADIAN RHYTHMICITY	The hypothesis that carbon acquisition in phaeophytes of lower organization is controlled by a blue-light-regulated C-4 mechanism was tested by various approaches with the filamentous species Ectocarpus siliculosus. Here we report analyses at the enzymatic level. Enzymes potentially participating in a C-4 cycle were assayed in extracts, and activities of a phosphoenolpyruvate carboxykinase (PEPCK), of NAD- and NADP-malate dehydrogenases, and of a NADP-malic enzyme were detected. No activity could be measured of phosphoenolpyruvate carboxylase or of pyruvate-Pi-dikinase, which is essential to complete the cycle by regenerating phosphoenolpyruvate from pyruvate. The kinetic properties of all enzymes were determined Malic enzyme and PEPCK were purified to homogeneity, as tested by gel filtration. The molecular mass of native malic enzyme was determined to be 440 kDa. In SDS gel electrophoreses it produced a single band with a relative molecular mass of 53 kDa, which suggested that the native molecule might be a homo-octamer. The molecular mass of PEPCK was 90 kDa and separation on SDS gels produced two major bands, one of 56 kDa and one of 18 kDa, which appeared not to be a degradation product of the larger polypeptide. The composition of the native enzyme is unclear. In extracts, the activities of malic enzyme and PEPCK followed a circadian rhythm, but none of the enzymes tested was found to be blue-light-dependent. The above results neither prove nor dismiss the possibility that a C-4 mechanism operates in Ectocarpus.	Univ Marburg, Fachbereich Biol Bot, D-35032 Marburg, Germany	Schmid, R (corresponding author), Univ Marburg, Fachbereich Biol Bot, D-35032 Marburg, Germany.						AKAGAWA H, 1972, PLANT CELL PHYSIOL, V13, P999; AXELSSON L, 1991, SEAWEED CELLULAR BIO; BLUM H, 1987, ELECTROPHORESIS, V8, P93, DOI 10.1002/elps.1150080203; Colombo SL, 1997, PHYSIOL PLANTARUM, V101, P821, DOI 10.1111/j.1399-3054.1997.tb01069.x; DAVISON IR, 1985, J PHYCOL, V21, P41; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; EDWARDS GE, 1992, PHYTOCHEMISTRY, V31, P1845, DOI 10.1016/0031-9422(92)80322-6; Edwards GE, 1981, BIOCH PLANTS COMPREH, P237; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; HATCH MD, 1973, ANAL BIOCHEM, V52, P280, DOI 10.1016/0003-2697(73)90350-3; HELDT HW, 1979, ENCY PLANT PHYSL, V6, P202; Hillrichs S, 2001, EUR J PHYCOL, V36, P71, DOI 10.1017/S096702620100302X; JOHNSTON AM, 1989, J PHYCOL, V25, P568, DOI 10.1111/j.1529-8817.1989.tb00263.x; JOHNSTON AM, 1991, CAN J BOT, V69, P1123, DOI 10.1139/b91-144; JOSHI G V, 1973, Proceedings of the Indian National Science Academy Part B Biological Sciences, V39, P489; Kawamitsu Y, 1999, MAR BIOL, V133, P361, DOI 10.1007/s002270050475; KERBY NW, 1985, ADV BOT RES, V11, P71, DOI 10.1016/S0065-2296(08)60169-X; KERBY NW, 1983, J PHYCOL, V19, P1, DOI 10.1111/j.0022-3646.1983.00001.x; KERBY NW, 1983, J PHYCOL, V19, P421, DOI 10.1111/j.0022-3646.1983.00421.x; Kremer B, 1981, OCEANOGR MAR BIOL AN, V19, P41; KREMER BP, 1981, PHYCOLOGIA, V20, P242, DOI 10.2216/i0031-8884-20-3-242.1; KREMER BP, 1977, PLANTA, V133, P191, DOI 10.1007/BF00391918; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; NAKAMOTO H, 1983, PLANT PHYSIOL, V71, P568, DOI 10.1104/pp.71.3.568; PETERSON GL, 1977, ANAL BIOCHEM, V83, P346, DOI 10.1016/0003-2697(77)90043-4; Plaxton WC, 1996, ANNU REV PLANT PHYS, V47, P185, DOI 10.1146/annurev.arplant.47.1.185; Provasoli L., 1968, CULTURES COLLECTIONS, P63; PUPILLO P, 1979, PLANTA, V144, P283, DOI 10.1007/BF00388771; REISKIND JB, 1989, AQUAT BOT, V33, P71, DOI 10.1016/0304-3770(89)90021-1; REISKIND JB, 1991, P NATL ACAD SCI USA, V88, P2883, DOI 10.1073/pnas.88.7.2883; SAITOU K, 1994, PLANT CELL PHYSIOL, V35, P1165, DOI 10.1093/oxfordjournals.pcp.a078710; SAITOU K, 1992, PLANT CELL PHYSIOL, V33, P595; Sakano K, 1998, PLANT CELL PHYSIOL, V39, P467, DOI 10.1093/oxfordjournals.pcp.a029393; SCAGLIARINI S, 1988, J EXP BOT, V39, P1109, DOI 10.1093/jxb/39.8.1109; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Schmid R, 1996, PLANT CELL ENVIRON, V19, P383, DOI 10.1111/j.1365-3040.1996.tb00330.x; Schmid R, 1996, SCI MAR, V60, P115; SCHMID R, 2001, IN PRESS EUR J PHYCO; Walker RP, 1997, AUST J PLANT PHYSIOL, V24, P459, DOI 10.1071/PP97007; Walker RP, 1996, BIOCHEM J, V317, P653, DOI 10.1042/bj3170653; WEDDING RT, 1989, PLANT PHYSIOL, V90, P367, DOI 10.1104/pp.90.2.367; Winter K., 1985, Photosynthetic mechanisms and the environment, P329	47	8	8	0	0	CAMBRIDGE UNIV PRESS	PORT CHESTER	110 MIDLAND AVE, PORT CHESTER, NY 10573-4930 USA	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	FEB	2001	36	1					61	70		10.1080/09670260110001735208			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	427FL	WOS:000168394100008					2021-04-07	
J	Hillrichs, S; Schmid, R				Hillrichs, S; Schmid, R			Activation by blue light of inorganic carbon acquisition for photosynthesis in Ectocarpus siliculosus: organic acid pools and short-term carbon fixation	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						blue light; beta-carboxylation; carbon fixation; carbon acquisition; organic acid pools; Phaeophyceae; photosynthesis	CYSTOSEIRA-ELEGANS SAUVAGEAU; BROWN-ALGAE; SATURATED PHOTOSYNTHESIS; CIRCADIAN RHYTHMICITY; STIMULATION; PHAEOPHYTA; TRANSPORT; MECHANISM; FUCALES	Blue light stimulates photosynthesis in brown algae by activating a mechanism for carbon acquisition. As this is connected with liberation of CO2 from an internal pool, it had previously been suggested that a C-4 pathway might operate, with CO2 mobilization h om an intermediate mediated by the action of blue light. The possibility that such a C-4 mechanism functions was tested in Ectocarpus siliculosus by investigating metabolite pools and carbon fixation products. Extracts were analysed by HPLC with an ion exchange column. With the exception of citrate/isocitrate all metabolite levels were far too low to serve as the intermediate pool. No changes in the concentrations of any of the metabolites were found after activation by blue light following red light pre-irradiation. During short-term incubations with C-14 inorganic carbon, the major label was found in phosphoglycerate. There was also clear labelling of peaks containing malate plus pyruvate and in citrate/isocitrate. In addition, glycerate contained high levels of radioactivity in some culture strains of Ectocarpus siliculosus. In chase experiments, the radioactivity of all metabolites continued to increase, indicating the presence of considerable amounts of intracellular inorganic carbon. Transient labelling was not observed for any of the fixation products, When Ectocarpus was pre-irradiated with red light and then stimulated by blue light, fixation rates increased with a time course that correlated with the activation of oxygen evolution. Photosynthetic oxygen evolution was investigated in vivo after the addition of possible intermediates to carbon-free seawater. Most of the exogenous applied substrates did not enhance photosynthesis, either in red light or after blue light stimulation. A strong enhancement seen after the addition of oxaloacetate was most likely due to release of CO2 during the breakdown of the metabolite in the medium. Taken together, the data argue strongly against the operation of a C-4 pathway in Ectocarpus. It is suggested that the internal pool mobilized by blue light contains inorganic carbon.	Univ Marburg, Fachbereich Biol Bot, D-35032 Marburg, Germany	Schmid, R (corresponding author), Univ Marburg, Fachbereich Biol Bot, D-35032 Marburg, Germany.						Badger MR, 1998, CAN J BOT, V76, P1052, DOI 10.1139/b98-074; Beer S, 1994, PROGR PHYCOLOGICAL R, V10, P179; Bowes G., 1987, Plant life in aquatic and amphibious habitats., P79; COUDRET A, 1987, CR ACAD SCI III-VIE, V305, P177; COUDRET A, 1985, CR ACAD SCI III-VIE, V301, P827; COUDRET A, 1992, PHOTOSYNTHETICA, V26, P235; DIONISIO ML, 1989, PLANT CELL PHYSIOL, V30, P215, DOI 10.1093/oxfordjournals.pcp.a077732; DIOURIS M, 1989, PHYCOLOGIA, V28, P504, DOI 10.2216/i0031-8884-28-4-504.1; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; Giraldez N, 1998, PHOTOCHEM PHOTOBIOL, V68, P420; JOHNSTON AM, 1991, CAN J BOT, V69, P1123, DOI 10.1139/b91-144; JOHNSTON AM, 1987, PHYCOLOGIA, V26, P159, DOI 10.2216/i0031-8884-26-2-159.1; KERBY NW, 1985, ADV BOT RES, V11, P71, DOI 10.1016/S0065-2296(08)60169-X; KOCH M, 1980, PHYTOCHEMISTRY, V19, P1821, DOI 10.1016/S0031-9422(00)83820-0; KREMER BP, 1981, PHYCOLOGIA, V20, P242, DOI 10.2216/i0031-8884-20-3-242.1; Provasoli L., 1968, CULTURES COLLECTIONS, P63; Raven JA, 1997, ADV BOT RES, V27, P85; Raven JA, 1997, EUR J PHYCOL, V32, P319, DOI 10.1080/09670269710001737259; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; Schmid R, 1998, PLANT CELL ENVIRON, V21, P523, DOI 10.1046/j.1365-3040.1998.00297.x; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Schmid R, 1996, PLANT CELL ENVIRON, V19, P383, DOI 10.1111/j.1365-3040.1996.tb00330.x; Schmid R, 1996, SCI MAR, V60, P115; SCHMID R, 2001, IN PRESS EUR J PHYCO	25	11	11	0	7	CAMBRIDGE UNIV PRESS	PORT CHESTER	110 MIDLAND AVE, PORT CHESTER, NY 10573-4930 USA	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	FEB	2001	36	1					71	79		10.1017/S096702620100302X			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	427FL	WOS:000168394100009		Bronze			2021-04-07	
J	Wang, M; Xu, YN; Jiang, GZ; Li, LB; Kuang, TY				Wang, M; Xu, YN; Jiang, GZ; Li, LB; Kuang, TY			Membrane lipids and their fatty acid composition in Nostoc flagelliforme cells	ACTA BOTANICA SINICA			Chinese	Article						Nostoc flagelliforme; membrane lipids; fatty acids; cold resistance	CYANOBACTERIUM SYNECHOCYSTIS PCC6803; ECTOCARPUS-FASCICULATUS PHAEOPHYCEAE; LOW-TEMPERATURE PHOTOINHIBITION; GENETIC MANIPULATION; CHILLING TOLERANCE; PHOSPHATIDYLGLYCEROL; PHOTOSYNTHESIS; UNSATURATION; DESATURATION; PLANTS	Nostoc flagelliforme Born. et Flah is highly adapted to drought stress, cold and light stresses, and suitable for growing in the unfavorable areas. This paper presents the results of the analysis of the membrane (mainly thylakoid membrane) lipids from N. flagelliforme in order to investigate the relationship between membrane lipid composition and stress resistance to this cyanobacteria. The membrane lipids are composed of monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), sulfoquinovosyl diacylglycerol (SQDG) and phosphatidylglycerol (PG). The major fatty acids in these lipids are palmitic (16:0), palmitoleic (16:1), stearic (18:0), oleic (18:1), linoleic (18:2) and linolenic(18:3) acids. In N. flagelliforme, polyunsaturated fatty acids account for 73% of the total fatty acids, much higher than that of the other cyanobacteria reported so far. Among which 16:1 and 18:3 are as high as 28.9% and 34.3% respectively. The high resistance of N. flagelliforme to abnormal conditions may be associated with the extent of unsaturation of fatty acids. In addition, the wild N. flagelliforme treated with water for 30 min and cultured for 24 h and the lipid and fatty acid composition were found to be not affected by water-absorption.	Chinese Acad Sci, Inst Bot, Lab Photosynth Basic Res, Beijing 100093, Peoples R China	Xu, YN (corresponding author), Chinese Acad Sci, Inst Bot, Lab Photosynth Basic Res, Beijing 100093, Peoples R China.						CUI ZY, 1985, ACTA PHYTOECOL GEOBO, V9, P220; GOMBOS Z, 1994, P NATL ACAD SCI USA, V91, P8787, DOI 10.1073/pnas.91.19.8787; GOMBOS Z, 1992, P NATL ACAD SCI USA, V89, P9959, DOI 10.1073/pnas.89.20.9959; GOSBOS Z, 1998, LIPIDS PHOTOSYNTHESI, P249; Makewicz A, 1997, PLANT CELL PHYSIOL, V38, P952, DOI 10.1093/oxfordjournals.pcp.a029257; MURATA N, 1983, PLANT CELL PHYSIOL, V24, P81, DOI 10.1093/oxfordjournals.pcp.a076516; MURATA N, 1984, ANACYSTIS NIDULANS, P337; Qian KX, 1989, ACTA PHYTOECOL GEOBO, V13, P97; ROUGHAN PG, 1985, PLANT PHYSIOL, V77, P740, DOI 10.1104/pp.77.3.740; RUSSELL NJ, 1984, TRENDS BIOCHEM SCI, V9, P108, DOI 10.1016/0968-0004(84)90106-3; Shi Ding-Ji, 1992, Acta Botanica Sinica, V34, P507; SIGENTHALER PA, 1998, LIPIDS PHOTOSYNTHESI, P145; Tasaka Y, 1996, EMBO J, V15, P6416, DOI 10.1002/j.1460-2075.1996.tb01033.x; WADA H, 1990, PLANT PHYSIOL, V92, P1062, DOI 10.1104/pp.92.4.1062; WADA H, 1992, PLANT CELL PHYSIOL, V33, P535; WADA H, 1994, P NATL ACAD SCI USA, V91, P4273, DOI 10.1073/pnas.91.10.4273; WADA H, 1990, NATURE, V347, P200, DOI 10.1038/347200a0; WANG X-L, 1984, Acta Botanica Sinica, V26, P484; Xu YN, 1999, FETT-LIPID, V101, P104; ZHANG XB, 1992, ARS COMBINATORIA, V34, P225; ZHU HR, 1982, J NANJING U NAT SCI, P117	21	12	15	0	3	SCIENCE PRESS	BEIJING	16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA	0577-7496			ACTA BOT SIN	Acta Bot. Sin.	DEC	2000	42	12					1263	1266					4	Biochemistry & Molecular Biology; Plant Sciences	Biochemistry & Molecular Biology; Plant Sciences	388BD	WOS:000166156800011					2021-04-07	
J	Maier, I; Parodi, E; Westermeier, R; Muller, DG				Maier, I; Parodi, E; Westermeier, R; Muller, DG			Maullinia ectocarpii gen. et sp nov (Plasmodiophorea), an intracellular parasite in Ectocarpus siliculosus (Ectocarpales, Phaeophyceae) and other filamentous brown algae	PROTIST			English	Article							E-FASCICULATUS PHAEOPHYCEAE; ULTRASTRUCTURE; BRASSICAE; CULTURE	An obligate intracellular parasite infecting Ectocarpus spp. and other filamentous marine brown algae is described. The pathogen forms an unwalled multinucleate syncytium (plasmodium) within the host cell cytoplasm and causes hypertrophy. Cruciform nuclear divisions occur during early development. Mature plasmodia become transformed into single sporangia, filling the host cell completely, and then cleave into several hundred spores. The spores are motile with two unequal, whiplash-type flagella inserted subapically and also show amoeboid movement. Upon settlement, cysts with chitinous walls are formed. Infection of host cells is accomplished by means of an adhesorium and a stachel apparatus penetrating the host cell wall, and injection of the cyst content into the host cell cytoplasm, The parasite is characterized by features specific for the plasmodiophorids and is described as a new genus and species, Maullinia ectocarpii.	Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany; UNS, CONICET, Inst Argentino Oceanog, RA-8000 Bahia Blanca, Argentina; Univ Austral Chile, Fac Pesquerias & Oceanog, Puerto Montt, Chile	Maier, I (corresponding author), Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany.						ADAMS MJ, 1988, DEV APPL BIOL, V2, P203; Aguilera M, 1988, GAYANA BOT, V45, P337; AIST JR, 1971, CAN J BOTANY, V49, P2023, DOI 10.1139/b71-284; ANDREWS JH, 1976, BIOL REV, V51, P211, DOI 10.1111/j.1469-185X.1976.tb01125.x; ARIF M, 1995, ANN APPL BIOL, V126, P493, DOI 10.1111/j.1744-7348.1995.tb05384.x; BARR DJS, 1982, CAN J BOT, V60, P2496, DOI 10.1139/b82-302; BARR DJS, 1992, MYCOLOGIA, V84, P1, DOI 10.2307/3760397; BARR DJS, 1988, DEV APPLIED BIOL, V2, P123; Borgesen F, 1926, DANSKE VIDENSK SELSK, V6, P1; BRASELTON JP, 1975, AM J BOT, V62, P349, DOI 10.2307/2442088; BRASELTON JP, 1975, ARCH MICROBIOL, V104, P97, DOI 10.1007/BF00447307; BRASELTON JP, 1990, CAN J BOT, V68, P594, DOI 10.1139/b90-078; BRASELTON JP, 1995, CRIT REV MICROBIOL, V21, P263, DOI 10.3109/10408419509113543; BRUNT AA, 1988, DEV APPL BIOL, V2, P3; BUCZACKI ST, 1983, ZOOSPORIC PLANT PATH, P161; Castlebury LA, 1998, MYCOLOGIA, V90, P102, DOI 10.2307/3761018; CAVALIERSMITH T, 1993, MICROBIOL REV, V57, P953, DOI 10.1128/MMBR.57.4.953-994.1993; CAVALIERSMITH T, 1993, J EUKARYOT MICROBIOL, V40, P609, DOI 10.1111/j.1550-7408.1993.tb06117.x; CORLISS JO, 1994, ACTA PROTOZOOL, V33, P1; Dawes C. J., 1974, MARINE ALGAE W COAST; den Hartog C, 1965, PERSOONIA, V4, P15; den Hartog C, 1963, GORTERIA, V1, P138; Dylewski D.P., 1990, P399; DYLEWSKI DP, 1984, PROTOPLASMA, V121, P42, DOI 10.1007/BF01279751; DYLEWSKI DP, 1978, AM J BOT, V65, P258, DOI 10.2307/2442265; GARBER RC, 1979, J CELL SCI, V40, P89; Hausmann K., 1996, PROTOZOOLOGY; Karling JS, 1944, AM J BOT, V31, P38, DOI 10.2307/2437666; KARLING JS, 1981, NOVA HEDWIGIA, V35, P17; Karling JS, 1968, PLASMODIOPHORALES; KESKIN B, 1971, ARCH MIKROBIOL, V77, P344, DOI 10.1007/BF00425037; KESKIN B, 1969, ARCH MIKROBIOL, V68, P218, DOI 10.1007/BF00409914; Kupper FC, 1999, NOVA HEDWIGIA, V69, P381; LUTHER H, 1949, MEM SOC FAUNA FLORA, V25, P88; MARZIANO F, 1995, MYCOTAXON, V55, P165; MILLER CE, 1985, CAN J BOT, V63, P263, DOI 10.1139/b85-030; Muller D.G., 1999, PHYCOL RES, V47, P217, DOI DOI 10.1111/J.1440-1835.1999.TB00301.X; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; Porter D., 1986, P141; PROVASOLI L, 1968, P US JAPAN C CULTURE, P63; ROBB EJ, 1982, SCIENCE, V218, P1221, DOI 10.1126/science.218.4578.1221; SCHNEPF E, 1994, BOT ACTA, V107, P374, DOI 10.1111/j.1438-8677.1994.tb00810.x; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; Silva P.C., 1996, CATALOGUE BENTHIC MA; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES; TALLEY MR, 1978, MYCOLOGIA, V70, P1241, DOI 10.2307/3759323; TEAKLE DS, 1983, ZOOSPORIC PLANT PATH, P233; WILLIAMS PH, 1970, PHYTOPATHOLOGY, V60, P1557, DOI 10.1094/Phyto-60-1557; WITTMANN W, 1965, STAIN TECHNOL, V40, P161, DOI 10.3109/10520296509116398; ZOPF W, 1894, BEITR PHYSL MORPH NI, V2, P43	52	31	32	0	10	URBAN & FISCHER VERLAG	JENA	BRANCH OFFICE JENA, P O BOX 100537, D-07705 JENA, GERMANY	1434-4610			PROTIST	Protist	OCT	2000	151	3					225	238		10.1078/1434-4610-00021			14	Microbiology	Microbiology	369CZ	WOS:000090155400002	11079768				2021-04-07	
J	Villarreal, LP; DeFilippis, VR				Villarreal, LP; DeFilippis, VR			A hypothesis for DNA viruses as the origin of eukaryotic replication proteins	JOURNAL OF VIROLOGY			English	Article							MARINE BROWN-ALGAE; ECTOCARPUS-SILICULOSUS; CELLULAR ONCOGENES; MOLECULAR-GENETICS; POLYMERASES; EVOLUTION; FASCICULATUS; PLASMIDS; ALPHA; LIFE	The eukaryotic replicative DNA polymerases are similar to those of large DNA viruses of eukaryotic and bacterial T4 phages but not to those of eubacteria. We develop and examine the hypothesis that DNA virus replication proteins gave rise to those of eukaryotes during evolution. We chose the DNA polymerase from phycodnavirus (which infects microalgae) as the basis of this analysis, as it represents a virus of a primitive eukaryote. We show that it has significant similarity with replicative DNA polymerases of eukaryotes and certain of their large DNA viruses. Sequence alignment confirms this similarity and establishes the presence of highly conserved domains in the polymerase amino terminus. Subsequent reconstruction of a phylogenetic tree indicates that these algal viral DNA polymerases are near the root of the clade containing all eukaryotic DNA polymerase delta members but that this clade does not contain the polymerases of other DNA viruses. We consider arguments for the polarity of this relationship and present the hypothesis that the replication genes of DNA viruses gave rise to those of eukaryotes and not the reverse direction.	Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92697 USA; Univ Calif Irvine, Dept Ecol & Evolut Biol, Irvine, CA 92697 USA	Villarreal, LP (corresponding author), Univ Calif Irvine, Dept Mol Biol & Biochem, 3205 Bio Sci II, Irvine, CA 92697 USA.						Afonso CL, 1999, J VIROL, V73, P533, DOI 10.1128/JVI.73.1.533-552.1999; Baldo AM, 1999, J VIROL, V73, P7710, DOI 10.1128/JVI.73.9.7710-7721.1999; BERNAD A, 1987, EMBO J, V6, P4219, DOI 10.1002/j.1460-2075.1987.tb02770.x; BERNSTEIN H, 1989, J BACTERIOL, V171, P2265, DOI 10.1128/jb.171.5.2265-2270.1989; BISHOP JM, 1983, ANNU REV BIOCHEM, V52, P301, DOI 10.1146/annurev.bi.52.070183.001505; BRAITHWAITE DK, 1993, NUCLEIC ACIDS RES, V21, P787, DOI 10.1093/nar/21.4.787; Edgell DR, 1997, J BACTERIOL, V179, P2632, DOI 10.1128/jb.179.8.2632-2640.1997; Forterre P, 1999, MOL MICROBIOL, V33, P457, DOI 10.1046/j.1365-2958.1999.01497.x; Goldbach Rob, 1994, P105; Kapp M, 1998, VIRUS GENES, V16, P111, DOI 10.1023/A:1007962112756; KNOLL AH, 1992, SCIENCE, V256, P622, DOI 10.1126/science.1585174; Knopf CW, 1998, VIRUS GENES, V16, P47, DOI 10.1023/A:1007997609122; Kutter E, 1995, VIRUS GENES, V11, P285, DOI 10.1007/BF01728666; Lake JA, 1999, SCIENCE, V283, P2027, DOI 10.1126/science.283.5410.2027; Mueller D. G., 1996, Phycologia, V35, P61; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; Mushegian AR, 1996, P NATL ACAD SCI USA, V93, P10268, DOI 10.1073/pnas.93.19.10268; Nicholas KB, 1997, GENEDOC ANAL VISUALI; PISANI FM, 1992, NUCLEIC ACIDS RES, V20, P2711, DOI 10.1093/nar/20.11.2711; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; SOGIN ML, 1989, SCIENCE, V243, P75, DOI 10.1126/science.2911720; SPICER EK, 1988, J BIOL CHEM, V263, P7478; STASKAWICZ BJ, 1995, SCIENCE, V268, P661, DOI 10.1126/science.7732374; SWOFFORD DL, 1993, J GEN PHYSIOL, V102, pA9; UEMORI T, 1995, J BACTERIOL, V177, P2164, DOI 10.1128/jb.177.8.2164-2177.1995; VANETTEN JL, 1994, ENCY VIROLOGY, V1, P35; VARMUS HE, 1984, ANNU REV GENET, V18, P553; Villareal LP, 1999, ORIGIN EVOLUTION VIR, P391; WANG CC, 1995, J BIOL CHEM, V270, P26558, DOI 10.1074/jbc.270.44.26558; WANG TSF, 1989, FASEB J, V3, P14; Zillig W, 1996, FEMS MICROBIOL REV, V18, P225, DOI 10.1111/j.1574-6976.1996.tb00239.x	32	147	154	0	14	AMER SOC MICROBIOLOGY	WASHINGTON	1752 N ST NW, WASHINGTON, DC 20036-2904 USA	0022-538X			J VIROL	J. Virol.	AUG	2000	74	15					7079	7084		10.1128/JVI.74.15.7079-7084.2000			6	Virology	Virology	333AE	WOS:000088105900042	10888648	Green Published, Bronze			2021-04-07	
J	Delaroque, N; Wolf, S; Muller, DG; Knippers, R				Delaroque, N; Wolf, S; Muller, DG; Knippers, R			The brown algal virus EsV-1 particle contains a putative hybrid histidine kinase	VIROLOGY			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; 2-COMPONENT REGULATORS; SIGNAL-TRANSDUCTION; ESCHERICHIA-COLI; PROTEIN; GENOME; EUKARYOTES; HOST; DNA; EXPRESSION	The Ectocarpus sillculosus virus, EsV-1, occurs worldwide in all populations of the filamentous marine brown alga E. siliculosus. We have screened an expression library of EsV-1 restriction fragments and identified a DNA clone with the potential to code for a 52-kDa histidine protein kinase. The derived amino acid sequence includes all homology boxes diagnostic for histidine protein kinases and, in addition, amino acid motifs that are commonly found in response regulators of bacterial two-component signal transduction proteins. Thus, the novel viral protein can be classified as a hybrid histidine protein kinase of a type that has previously been detected in fungi, slime molds, and plants. By using purified antibodies, we found that the protein with its potential kinase activity is located on the outer shell of viral particles. This is the first report on a two-component regulator-like protein in viruses and could provide the basis for speculations with regard to the evolution of EsV-1 and related viruses. (C) 2000 Academic Press.	Univ Konstanz, Dept Biol, D-78457 Constance, Germany	Delaroque, N (corresponding author), Univ Konstanz, Dept Biol, D-78457 Constance, Germany.						ALEX LA, 1994, TRENDS GENET, V10, P133, DOI 10.1016/0168-9525(94)90215-1; ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Blattner FR, 1997, SCIENCE, V277, P1453, DOI 10.1126/science.277.5331.1453; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; CHANG C, 1994, RES MICROBIOL, V145, P481, DOI 10.1016/0923-2508(94)90097-3; CHANG C, 1993, SCIENCE, V262, P539, DOI 10.1126/science.8211181; CHARLES TC, 1992, ANNU REV PHYTOPATHOL, V30, P463, DOI 10.1146/annurev.py.30.090192.002335; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; Delaroque N, 2000, VIROLOGY, V269, P148, DOI 10.1006/viro.2000.0225; Grebe TW, 1999, ADV MICROB PHYSIOL, V41, P139, DOI 10.1016/S0065-2911(08)60167-8; HARLOW E, 1988, ANTIBODIES LAB HDB; Hoch J.A., 1995, 2 COMPONENT SIGNAL T; HRABAK EM, 1992, J BACTERIOL, V174, P3011, DOI 10.1128/JB.174.9.3011-3020.1992; Jones DT, 1999, J MOL BIOL, V292, P195, DOI 10.1006/jmbi.1999.3091; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; Loomis WF, 1997, J CELL SCI, V110, P1141; MEKALANOS JJ, 1992, J BACTERIOL, V174, P1; Mossi R, 1998, EUR J BIOCHEM, V254, P209, DOI 10.1046/j.1432-1327.1998.2540209.x; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; NAGASAWA S, 1992, MOL MICROBIOL, V6, P799, DOI 10.1111/j.1365-2958.1992.tb01530.x; OTA IM, 1993, SCIENCE, V262, P566, DOI 10.1126/science.8211183; PARKINSON JS, 1992, ANNU REV GENET, V26, P71, DOI 10.1146/annurev.ge.26.120192.000443; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; SOUTHERN EM, 1975, J MOL BIOL, V98, P503, DOI 10.1016/S0022-2836(75)80083-0; STOCK AM, 1989, NATURE, V337, P745, DOI 10.1038/337745a0; STUDIER FW, 1986, J MOL BIOL, V189, P113, DOI 10.1016/0022-2836(86)90385-2; Tanaka T, 1998, NATURE, V396, P88; TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P4350, DOI 10.1073/pnas.76.9.4350; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; VENABLE JH, 1965, J CELL BIOL, V25, P407, DOI 10.1083/jcb.25.2.407; Wolf' S, 1998, PROTOPLASMA, V203, P153, DOI 10.1007/BF01279472	37	8	8	0	3	ACADEMIC PRESS INC	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA	0042-6822			VIROLOGY	Virology	AUG 1	2000	273	2					383	390		10.1006/viro.2000.0383			8	Virology	Virology	346AE	WOS:000088847300018	10915609				2021-04-07	
J	Ouriques, LC; Bouzon, ZL				Ouriques, LC; Bouzon, ZL			Stellate chloroplast organization in Asteronema breviarticulatum comb. nov (Ectocarpales, Phaeophyta)	PHYCOLOGIA			English	Article							ARRANGEMENT	Light and electron microscope studies of vegetative cells of Asteronema breviarticulatum (J. Agardh) Ouriques & Bouzon comb. nov. demonstrated that groups of plastids are organized in a stellate arrangement. Each plastid has a terminal pyrenoid, and the centre of a group of chloroplasts is formed by a cluster of all the pyrenoids. The individual pyrenoids are separated by a thin interpyrenoid space. These cytological traits distinguish A. breviarticulatum from the genera Ectocarpus and Hincksia, in which this species has previously been classified. Morphological and reproductive characters within the genera of small brown algae with a stellate plastid arrangement (i.e. Bachelotia. Asterocladon. and Asteronema.) also suggest that the new combination A. breviarticulatum is justified.	Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Biol Celular Embriol & Genet, BR-88049900 Florianopolis, SC, Brazil	Ouriques, LC (corresponding author), Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Biol Celular Embriol & Genet, BR-88049900 Florianopolis, SC, Brazil.		bouzon, zenilda/I-6673-2012				Agardh J.G., 1847, OFVERSIGT KONGLIGA S, V4, P5; Borgesen F., 1914, DANSK BOT ARK, V2, P1, DOI DOI 10.5962/BHL.TITLE.1314; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; CARDINAL A, 1967, NOVA HEDWIGIA, V15, P1; CHUNG IK, 1987, KOREAN J PHYCOLOGY, V2, P147; CLAYTON MN, 1974, AUST J BOT, V22, P743, DOI 10.1071/BT9740743; DELEPINE R, 1976, Phycologia, V15, P425, DOI 10.2216/i0031-8884-15-3-425.1; DELEPINE R, 1975, B SOC BOT FR, V122, P295, DOI 10.1080/00378941.1975.10835618; HORI T, 1971, BOT MAG TOKYO, V84, P231; JOLY AB, 1965, B FACULDADE FILOSO B, V2, P1; Lawson G.W., 1982, NOVA HEDWIGIA, V70, P1; MAGNE F, 1976, Phycologia, V15, P309, DOI 10.2216/i0031-8884-15-3-309.1; MULLER DG, 1994, PHYCOLOGIA, V33, P471, DOI 10.2216/i0031-8884-33-6-471.1; Muller DG, 1998, PHYCOLOGIA, V37, P425, DOI 10.2216/i0031-8884-37-6-425.1; Oliveira EC., 1995, MANUAL METODOS FICOL, P429; OURIQUES LC, 1997, THESIS U STATE SAO P; Peters AF, 1998, PHYCOLOGIA, V37, P106, DOI 10.2216/i0031-8884-37-2-106.1; PHAMHOANGHO, 1969, RONG VIETNAM; Silva P.C., 1987, SMITHSON CONTRIB MAR, V27, P1, DOI DOI 10.5479/SI.1943667X.27.1; Silva P.C., 1996, U CALIF PUBL BOT, V79, P1, DOI DOI 10.2216/i0031-8884-38-4-342b.1; SZECHY M T M D, 1991, Hoehnea, V18, P205; Taylor WR, 1960, MARINE ALGAE E TROPI; Ugadim Y., 1973, ACTA BIOL, V12, P69; Wynne M.J., 1998, NOVA HEDWIGIA S, V116, P1	24	10	16	0	6	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	JUL	2000	39	4					267	271		10.2216/i0031-8884-39-4-267.1			5	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	380WH	WOS:000165726100001					2021-04-07	
J	Wolf, S; Muller, DG; Maier, I				Wolf, S; Muller, DG; Maier, I			Assembly of a large icosahedral DNA virus, MclaV-1, in the marine alga Myriotrichia clavaeformis (Dictyosiphonales, Phaeophyceae)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						algae; assembly; electron microscopy; Myriotrichia clavaeformis; marine dsDNA virus; Phaeophyceae; phycovirus	SWINE FEVER VIRUS; ECTOCARPUS-FASCICULATUS ECTOCARPALES; ENDOPLASMIC-RETICULUM; SILICULOSUS PHAEOPHYCEAE; HINCKSIA-HINCKSIAE; EUKARYOTIC ALGAE; BROWN-ALGAE; AFRICAN; CULTURE; INFECTION	The marine brown alga Myriotrichia clavaeformis (Dictyosiphonales) is the host of a large icosahedral virus with a double-stranded DNA genome, MclaV-1. This pathogen replicates exclusively in prospective reproductive organs of the alga. We studied the assembly of virus particles by transmission electron microscopy. Replication of viral DNA starts in the nucleus, which hypertrophies and later disintegrates. Capsid assembly begins in the cytoplasm by budding from virus-detaching bodies. After nuclear breakdown, assembly continues in a mixed cyto-/nucleoplasm on membrane cisternae, which probably originate from the endoplasmic reticulum. Virus particles thereby acquire as an integral capsid component a membrane to which proteins are apposed. Material inside the capsids partly condenses to form an additional layer in the core shell. DNA is packaged after capsid formation giving rise to an electron-opaque nucleoprotein core. hi. clavaeformis infected by MclaV-1 is the second brown aigal host-virus system in which virus assembly has been studied in detail. Together with previous observations, our results allow conclusions on general mechanisms of virus assembly in brown algae. Some features of virus formation in brown algae show similarities with large icosahedral DNA viruses infecting animal cells.	Univ Konstanz, Fak Biol, D-78457 Constance, Germany	Wolf, S (corresponding author), Univ Konstanz, Fak Biol, D-78457 Constance, Germany.						Andres G, 1997, J VIROL, V71, P2331; Andres G, 1998, J VIROL, V72, P8988; Brookes SM, 1998, VIROLOGY, V249, P175, DOI 10.1006/viro.1998.9308; Brookes SM, 1996, VIROLOGY, V224, P84, DOI 10.1006/viro.1996.0509; CLITHEROE SB, 1974, J ULTRA MOL STRUCT R, V49, P211, DOI 10.1016/S0022-5320(74)80032-8; Cobbold C, 1996, J VIROL, V70, P8382, DOI 10.1128/JVI.70.12.8382-8390.1996; Cobbold C, 1998, J VIROL, V72, P5215, DOI 10.1128/JVI.72.6.5215-5223.1998; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; DUBE P, 1993, EMBO J, V12, P1303, DOI 10.1002/j.1460-2075.1993.tb05775.x; Etten J. L. van, 1991, Seminars in Virology, V2, P71; Garcia-Escudero R, 1998, J VIROL, V72, P3185; GARCIABEATO R, 1992, VIROLOGY, V188, P637, DOI 10.1016/0042-6822(92)90518-T; GERSHON AA, 1994, J VIROL, V68, P6372, DOI 10.1128/JVI.68.10.6372-6390.1994; GOORHA R, 1982, J VIROL, V43, P519, DOI 10.1128/JVI.43.2.519-528.1982; Griffiths G, 1992, Semin Cell Biol, V3, P367, DOI 10.1016/1043-4682(92)90022-N; HENDRIX RW, 1994, SEMIN VIROL, V5, P15, DOI 10.1006/smvy.1994.1003; Hendrix RW, 1998, CELL, V94, P147, DOI 10.1016/S0092-8674(00)81413-0; HESS RT, 1985, CURR TOP MICROBIOL, V116, P49; HURLEY EA, 1991, J VIROL, V65, P1245, DOI 10.1128/JVI.65.3.1245-1254.1991; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; Maier I, 1997, J PHYCOL, V33, P838, DOI 10.1111/j.0022-3646.1997.00838.x; Maier I, 1998, EUR J PHYCOL, V33, P213, DOI 10.1017/S0967026298001747; MEINTS RH, 1986, VIROLOGY, V154, P240, DOI 10.1016/0042-6822(86)90448-4; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, PROTOPLASMA, V193, P58, DOI 10.1007/BF01276634; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; Murphy F. A, 1995, VIRUS TAXONOMY; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; PETERS AF, 1988, BRIT PHYCOL J, V23, P299, DOI 10.1080/00071618800650331; REISSER W, 1993, ARCH PROTISTENKD, V143, P257, DOI 10.1016/S0003-9365(11)80293-9; REISSER W, 1995, ALGAE ENV HUMAN AFFA, P143; Roizman Bernard, 1993, P11; Rouiller I, 1998, J VIROL, V72, P2373, DOI 10.1128/JVI.72.3.2373-2387.1998; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VENABLE JH, 1965, J CELL BIOL, V25, P407, DOI 10.1083/jcb.25.2.407; Williams T, 1996, ADV VIRUS RES, V46, P345, DOI 10.1016/S0065-3527(08)60076-7; Wolf' S, 1998, PROTOPLASMA, V203, P153, DOI 10.1007/BF01279472	39	11	11	0	1	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	MAY	2000	35	2					163	171		10.1017/S0967026200002663			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	336XK	WOS:000088328100008		Bronze			2021-04-07	
J	Cruz-Rivera, E; Hay, ME				Cruz-Rivera, E; Hay, ME			The effects of diet mixing on consumer fitness: macroalgae, epiphytes, and animal matter as food for marine amphipods	OECOLOGIA			English	Article						amphipods; dietary mixing; fitness; herbivores; occasional carnivory	GENERALIST HERBIVORE; CHEMICAL DEFENSE; COMPLEMENTARY RESOURCES; GAMMARUS-MUCRONATUS; RELATIVE IMPORTANCE; INSECT EQUIVALENTS; SALT-MARSH; EGG SIZE; GROWTH; PREDATION	Herbivores are thought to achieve adequate nutrition by consuming numerous species of plants or by occasionally consuming animal tissue. Although active selection of diverse foods is common in nature, the relationship between diet mixing and consumer fitness is poorly understood, especially in marine environments. We studied the fitness-based consequences of dietary mixing in the sympatric amphipods Ampithoe marcuzzii, A. valida, Cymadusa compta, and Gammarus mucronatus by measuring survivorship, growth, and fecundity of these amphipods when they were offered single species of algae, a single animal food, a mixture of algal species, or a combination of algae and animal matter. For the more sedentary, tube-building amphipods A. marcuzzii, A. valida, and C. compta, fitness on mixed algal diets was matched by fitness on at least one of the monospecific algal diets, suggesting that they could benefit from preferential feeding on those algae in the field. The more mobile amphipod, G. mucronatus, survived and grew similarly on the mixed diets and on the filamentous brown alga Ectocarpus siliculosus. However, its fecundity was significantly higher when feeding on the algal and animal mixture than on Ectocarpus alone. Additionally, for G. mucronatus, fitness on mixed algae, mixed algae plus animal matter, and animal matter alone was equivalent, although female growth (but not gonad production) was slightly lower on animal matter alone than on the mixed algae combined with animal food. Thus the more mobile amphipod, G. mucronatus, was the only species able to perform well on animal food alone. In contrast, A. valida and C. compta experienced large negative effects when limited to consuming animal matter alone. For these two species, combining algae and animal matter did not enhance fitness over combining only algae. Fitness effects of specific algal diets showed some general similarities, but also considerable variance among the amphipods. For example, E. siliculosus was generally better food than other algae for all four amphipods, whereas Sargassum filipendula was generally poor. However, A. marcuzzii did not suffer negative effects of consuming only Sargassum. The red alga Polysiphonia sp. and the green alga Enteromorpha flexuosa decreased fitness in A. marcuzzii, C. compta, and G. mucronatus, but not A. valida, and the negative effects of Polysiphonia were considerably larger for A. marcuzzii than for the other amphipods. Our data show that nutritional requirements, even among related species (e.g., A. marcuzzii and A. valida), can be dramatically different. Diet mixing may benefit more mobile consumers like Gammarus that are better able to search for different foods, and may be less important for more sedentary herbivores like Ampithoe and Cymadusa that consume, and live in close association with, individual host plants.	Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA; Univ N Carolina, Inst Marine Sci, Morehead City, NC 28557 USA	Hay, ME (corresponding author), Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA.	mark.hay@biology.gatech.edu	Hay, Mark E/D-9752-2011	Hay, Mark E/0000-0002-6130-9349; Cruz-Rivera, Edwin/0000-0002-5376-9863			AGNEW DJ, 1986, J EXP MAR BIOL ECOL, V103, P203, DOI 10.1016/0022-0981(86)90141-3; Alonso G., 1995, Oebalia, V21, P77; BARLOCHER F, 1986, J EXP MAR BIOL ECOL, V104, P229, DOI 10.1016/0022-0981(86)90107-3; BARNARD JL, 1991, REC AUST MUS S, P13; BAYNE BL, 1978, J MAR BIOL ASSOC UK, V53, P673; BELL SS, 1991, ECOLOGY, V72, P350, DOI 10.2307/1938929; BELOVSKY GE, 1984, AM NAT, V124, P97, DOI 10.1086/284254; BERNAYS E, 1988, ECOLOGY, V69, P886, DOI 10.2307/1941237; Bernays EA, 1997, ECOLOGY, V78, P1157, DOI 10.1890/0012-9658(1997)078[1157:IHDRFB]2.0.CO;2; BERNAYS EA, 1994, ECOLOGY, V75, P1997, DOI 10.2307/1941604; BJORNDAL KA, 1991, ECOLOGY, V72, P1234, DOI 10.2307/1941097; Bousfield E.L., 1973, SHALLOW WATER GAMMAR; BRAWLEY SH, 1992, SYST ASSOC SPEC VOL, V46, P235; BRODY MS, 1984, OECOLOGIA, V61, P55, DOI 10.1007/BF00379089; CAPINERA JL, 1979, AM NAT, V114, P350, DOI 10.1086/283484; Courtney S.P., 1990, P161; CRUZRIVERA E, IN PRESS ECOLOGY; DANTONIO C, 1985, J EXP MAR BIOL ECOL, V86, P197, DOI 10.1016/0022-0981(85)90103-0; DEAL MS, 1997, THESIS U N CAROLINA; DEARING MD, 1992, ECOLOGY, V73, P845, DOI 10.2307/1940162; DELONG MD, 1993, CAN J FISH AQUAT SCI, V50, P1891, DOI 10.1139/f93-211; DeMott WR, 1998, ECOLOGY, V79, P2463, DOI 10.1890/0012-9658(1998)079[2463:UOACAA]2.0.CO;2; DUFFY JE, 1991, ECOLOGY, V72, P1286, DOI 10.2307/1941102; DUFFY JE, 1990, OECOLOGIA, V83, P267, DOI 10.1007/BF00317764; DUFFY JE, 1994, ECOLOGY, V75, P1304, DOI 10.2307/1937456; DUFFY JE, 1991, ECOLOGY, V72, P354, DOI 10.2307/1938930; DUFFY JE, IN PRESS ECOL MONOGR; FREDETTE TJ, 1986, J CRUSTACEAN BIOL, V6, P57, DOI 10.2307/1547931; FREELAND WJ, 1974, AM NAT, V108, P269, DOI 10.1086/282907; FUTUYMA DJ, 1988, ANNU REV ECOL SYST, V19, P207, DOI 10.1146/annurev.ecolsys.19.1.207; GRACA MAS, 1993, OECOLOGIA, V96, P304, DOI 10.1007/BF00317498; GREENSTONE MH, 1979, NATURE, V282, P501, DOI 10.1038/282501a0; Gulati RD, 1997, FRESHWATER BIOL, V38, P753, DOI 10.1046/j.1365-2427.1997.00275.x; Hauxwell J, 1998, ESTUARIES, V21, P347, DOI 10.2307/1352481; HAWKINS SJ, 1983, OCEANOGR MAR BIOL, V21, P195; Hay M.E., 1992, HERBIVORES THEIR INT, P372; Hay Mark E., 1992, P93; Hay ME, 1996, J EXP MAR BIOL ECOL, V200, P103, DOI 10.1016/S0022-0981(96)02659-7; HAY ME, 1990, ECOLOGY, V71, P733, DOI 10.2307/1940326; HAY ME, 1987, ECOLOGY, V68, P1567, DOI 10.2307/1939849; HAY ME, 1988, MAR ECOL PROG SER, V48, P185, DOI 10.3354/meps048185; HAY ME, 1981, ECOLOGY, V62, P1395, DOI 10.2307/1937304; HAY ME, 1988, OECOLOGIA, V75, P246, DOI 10.1007/BF00378605; HELM MM, 1973, J MAR BIOL ASSOC UK, V53, P673, DOI 10.1017/S0025315400058872; HUTCHINGS JA, 1991, EVOLUTION, V45, P1162, DOI [10.2307/2409723, 10.1111/j.1558-5646.1991.tb04382.x]; Jernakoff P, 1996, OCEANOGR MAR BIOL, V34, P109; Jernakoff P, 1997, AQUAT BOT, V56, P183, DOI 10.1016/S0304-3770(96)01112-6; JOHNSON GD, 1990, J WILDLIFE MANAGE, V54, P89, DOI 10.2307/3808906; KAPLAN RH, 1992, ECOLOGY, V73, P280, DOI 10.2307/1938739; KARLSSON B, 1984, OIKOS, V43, P376, DOI 10.2307/3544156; Kennish R, 1996, OECOLOGIA, V105, P22, DOI 10.1007/BF00328787; KITTING CL, 1980, ECOL MONOGR, V50, P527, DOI 10.2307/1942656; Kneib RT, 1997, MAR BIOL, V128, P423, DOI 10.1007/s002270050108; KREBS JR, 1984, OECOLOGIA, V64, P363, DOI 10.1007/BF00379134; LAFRANCE K, 1985, LIMNOL OCEANOGR, V30, P1067, DOI 10.4319/lo.1985.30.5.1067; LARSON BR, 1980, MAR BIOL, V59, P49, DOI 10.1007/BF00396982; Leather S. R., 1994, INSECT PLANT INTERAC, P175; LEWIS FG, 1984, MAR ECOL PROG SER, V19, P101, DOI 10.3354/meps019101; LOBEL PS, 1981, MAR BIOL, V64, P173, DOI 10.1007/BF00397106; MACFARLANE JH, 1980, ACRIDA, V9, P63; MacNeil C, 1997, BIOL REV, V72, P349, DOI 10.1017/S0006323196005038; Martin M.M., 1987, INVERTEBRATE MICROBI; MATTSON WJ, 1980, ANNU REV ECOL SYST, V11, P119, DOI 10.1146/annurev.es.11.110180.001003; MCTIGUE TA, 1991, J EXP MAR BIOL ECOL, V151, P1, DOI 10.1016/0022-0981(91)90011-K; MUKAI H, 1995, ECOL RES, V10, P243, DOI 10.1007/BF02347850; NELSON WG, 1979, J EXP MAR BIOL ECOL, V39, P231, DOI 10.1016/0022-0981(79)90129-1; NELSON WG, 1979, J EXP MAR BIOL ECOL, V38, P225, DOI 10.1016/0022-0981(79)90069-8; NICOTRI ME, 1977, AQUACULTURE, V12, P127, DOI 10.1016/0044-8486(77)90179-X; NICOTRI ME, 1980, J EXP MAR BIOL ECOL, V42, P13, DOI 10.1016/0022-0981(80)90163-X; ODOWD DJ, 1980, ECOLOGY, V61, P531, DOI 10.2307/1937419; ORTH RJ, 1984, AQUAT BOT, V18, P43, DOI 10.1016/0304-3770(84)90080-9; PEDERSON JB, 1984, J EXP MAR BIOL ECOL, V76, P277, DOI 10.1016/0022-0981(84)90193-X; PENNINGS SC, 1993, ECOLOGY, V74, P879, DOI 10.2307/1940813; POMEROY WM, 1980, CAN J FISH AQUAT SCI, V37, P1, DOI 10.1139/f80-001; Poore AGB, 1999, ECOL MONOGR, V69, P443, DOI 10.1890/0012-9615(1999)069[0443:PPRAEO]2.0.CO;2; PRICE PW, 1980, ANNU REV ECOL SYST, V11, P41, DOI 10.1146/annurev.es.11.110180.000353; Randall J. E., 1967, STUD TROP OCEANOGR U, V5, P655; ROBERTSON AI, 1983, J EXP MAR BIOL ECOL, V72, P99, DOI 10.1016/0022-0981(83)90138-7; Rodriguez J.G, 1987, NUTR ECOLOGY INSECTS; ROGERS CN, 1995, J EXP MAR BIOL ECOL, V192, P47, DOI 10.1016/0022-0981(95)00057-X; SANDERS HL, 1962, LIMNOL OCEANOGR, V7, P63, DOI 10.4319/lo.1962.7.1.0063; Sanders RW, 1996, LIMNOL OCEANOGR, V41, P1295, DOI 10.4319/lo.1996.41.6.1295; Schmidt K, 1997, MAR ECOL PROG SER, V151, P1, DOI 10.3354/meps151001; Simpson S.J., 1990, P111; Slansky Frank Jr., 1993, P29; Smith HG, 1998, OECOLOGIA, V115, P59, DOI 10.1007/s004420050491; SPEISER B, 1991, OIKOS, V62, P306, DOI 10.2307/3545495; Stachowicz JJ, 1999, ECOLOGY, V80, P495, DOI 10.1890/0012-9658(1999)080[0495:RPTCMC]2.0.CO;2; Stachowicz JJ, 1996, OECOLOGIA, V105, P377, DOI 10.1007/BF00328741; STEINBERG PD, 1992, ECOL MONOGR, V62, P189, DOI 10.2307/2937093; STERNER RW, 1994, ANNU REV ECOL SYST, V25, P1, DOI 10.1146/annurev.es.25.110194.000245; STONER AW, 1979, MAR BIOL, V55, P201, DOI 10.1007/BF00396819; Toft S, 1999, OECOLOGIA, V119, P191, DOI 10.1007/s004420050776; Van Soest P.J., 1994, NUTR ECOLOGY RUMINAN, Vsecond; VANCE RR, 1979, OECOLOGIA, V44, P21, DOI 10.1007/BF00346391; WAHL M, 1995, OECOLOGIA, V102, P329, DOI 10.1007/BF00329800; WATANABE JM, 1984, OECOLOGIA, V62, P47, DOI 10.1007/BF00377371; White T.C.R., 1993, INADEQUATE ENV NITRO; WILLIAMS TD, 1994, BIOL REV, V68, P35, DOI DOI 10.1111/J.1469-185X.1994.TB01485.X; ZIMMERMAN R, 1979, MAR BIOL, V54, P41, DOI 10.1007/BF00387050	100	110	115	4	47	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0029-8549			OECOLOGIA	Oecologia	MAY	2000	123	2					252	264		10.1007/s004420051012			13	Ecology	Environmental Sciences & Ecology	321LW	WOS:000087457800013	28308730				2021-04-07	
J	Dixon, NM; Leadbeater, BSC; Wood, KR				Dixon, NM; Leadbeater, BSC; Wood, KR			Frequency of viral infection in a field population of Ectocarpus fasciculatus (Ectocarpales, Phaeophyceae)	PHYCOLOGIA			English	Article							MARINE BROWN-ALGAE; SILICULOSUS PHAEOPHYCEAE; VIRUS-INFECTIONS	Filaments of Ectocarpus fasciculatus epiphytic on Laminaria digitarn were sampled monthly from the rocky shore at Peveril Point, Swanage, UK, for more than a year and were screened for the presence or absence of overt viral infection. The mean levels of overtly infected filaments recorded per month ranged from 1.1 +/- 0.1% in June 1998 to 4.7 +/- 0.2% in February 1999. Significantly higher levels of overt infection occurred during the winter months, and a significant negative correlation existed between this fluctuation and the surface sea temperature. In addition, a highly significant spatial difference was determined in the overt infection incidence on six reef faces.	Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England	Dixon, NM (corresponding author), Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England.						BOALCH GT, 1961, J MAR BIOL ASSOC UK, V41, P287, DOI 10.1017/S0025315400023912; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; GILLMAN M, 1997, METHODS ECOLOGY INTR; GRUBB VM, 1936, J ECOL, V24, P391; HARRIS EA, 1999, THESIS U BIRMINGHAM; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; MULLER DG, 1972, PHYCOLOGIA, V11, P11; MULLER DG, 1964, NATURE, V303, P1402; MULLER DIETER, 1962, BOT MARINA, V4, P140, DOI 10.1515/botm.1962.4.1-2.140; PAPENFUSS GEORGE F., 1935, BOT GAZ, V96, P421, DOI 10.1086/334493; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; RUSSELL G, 1983, MAR ECOL PROG SER, V11, P181, DOI 10.3354/meps011181; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; Smith GM, 1942, AM J BOT, V29, P645, DOI 10.2307/2437177; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	20	4	4	0	2	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	MAY	2000	39	3					258	263		10.2216/i0031-8884-39-3-258.1			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	363YJ	WOS:000089865000010					2021-04-07	
J	Giraldez, N; Aparicio, PJ; Quinones, MA				Giraldez, N; Aparicio, PJ; Quinones, MA			Limiting CO2 levels induce a blue light-dependent HCO3- uptake system in Monoraphidium braunii	JOURNAL OF EXPERIMENTAL BOTANY			English	Article						blue light; HCO3- transport; CO2 concentration; Monoraphidium braunii; photosynthesis; protein biosynthesis	INORGANIC CARBON TRANSPORT; ALGAE SCENEDESMUS-OBLIQUUS; CHLAMYDOMONAS-REINHARDTII; GREEN-ALGAE; DUNALIELLA-TERTIOLECTA; ECTOCARPUS-SILICULOSUS; ANHYDRASE EXPRESSION; CHLORELLA-REGULARIS; PROTEIN-SYNTHESIS; PLASMA-MEMBRANE	The in situ photoactivation of an HCO3- uptake system in the green alga Monoraphidium braunii requires the irradiation of the cell suspensions with short wavelength radiation (blue, UVA and/or UVC), Plasma membrane ATPase inhibitors block the uptake of this monovalent anion at pH 9, M, braunii cells grown in high CO2 lack an HCO3- uptake system in their plasma membrane, but those grown in low CO2 can take up this anion at high rates. Cells grown in high CO2, transferred to CO2-limiting conditions in the light, start taking up HCO3- in 30 min, although they take 90 min to reach maximum rates of HCO3- transport. Therefore, this induction process seems to be triggered by low external CO2 concentration. In fact, increasing or decreasing the external HCO3- concentration does not induce the uptake system and only a decrease in CO2 concentration in the medium triggers the induction process. The appearance of the HCO3- transport activity is sensitive to cycloheximide, indicating that cytoplasmic protein biosynthesis is necessary for the induction of the uptake system. Photosynthetically active radiation, but not particularly blue light, is essential for induction of the uptake system to occur and the inhibition of photosynthesis by DCMU blocks it. From these results it can be inferred that when M. braunii cells detect a drop in CO2 concentration, they induce a blue light-dependent HCO3- uptake system.	CSIC, Ctr Invest Biol, E-28006 Madrid, Spain	Quinones, MA (corresponding author), CSIC, Ctr Invest Biol, Velazquez 144, E-28006 Madrid, Spain.		Quinones, Miguel/AAB-3714-2019	Quinones, Miguel/0000-0002-8814-0440			AIZAWA K, 1986, FEMS MICROBIOL LETT, V39, P215, DOI 10.1016/0378-1097(86)90447-7; Amoroso G, 1998, PLANT PHYSIOL, V116, P193, DOI 10.1104/pp.116.1.193; APARICIO PJ, 1994, PLANT CELL ENVIRON, V17, P1323, DOI 10.1111/j.1365-3040.1994.tb00534.x; BADGER MR, 1992, PHYSIOL PLANTARUM, V84, P606, DOI 10.1111/j.1399-3054.1992.tb04711.x; BADGER MR, 1994, ANNU REV PLANT PHYS, V45, P369, DOI 10.1146/annurev.pp.45.060194.002101; BAILLY J, 1988, PLANT PHYSIOL, V87, P833, DOI 10.1104/pp.87.4.833; BALKE NE, 1979, PLANT PHYSIOL, V63, P53, DOI 10.1104/pp.63.1.53; CANTLEY LC, 1978, J BIOL CHEM, V253, P7361; COLEMAN JR, 1991, PLANT CELL ENVIRON, V14, P861, DOI 10.1111/j.1365-3040.1991.tb01449.x; COLEMAN JR, 1984, P NATL ACAD SCI-BIOL, V81, P6049, DOI 10.1073/pnas.81.19.6049; DIONISIO ML, 1989, PLANT CELL PHYSIOL, V30, P215, DOI 10.1093/oxfordjournals.pcp.a077732; DIONISIOSESE ML, 1990, PLANT PHYSIOL, V94, P1103, DOI 10.1104/pp.94.3.1103; Eriksson M, 1996, P NATL ACAD SCI USA, V93, P12031, DOI 10.1073/pnas.93.21.12031; FINDENEGG GR, 1979, PLANT SCI LETT, V17, P101, DOI 10.1016/0304-4211(79)90168-8; FUKUZAWA H, 1990, P NATL ACAD SCI USA, V87, P4383, DOI 10.1073/pnas.87.11.4383; GALLAGHER SR, 1982, PLANT PHYSIOL, V70, P1335, DOI 10.1104/pp.70.5.1335; Giraldez N, 1998, PHOTOCHEM PHOTOBIOL, V68, P420; GOYAL A, 1989, PLANT PHYSIOL, V89, P1264, DOI 10.1104/pp.89.4.1264; Jenkins GI, 1995, PLANT SCI, V112, P117, DOI 10.1016/0168-9452(95)04260-1; KARLSSON J, 1994, PLANTA, V192, P46; KAUFMAN LS, 1993, PLANT PHYSIOL, V102, P333, DOI 10.1104/pp.102.2.333; Maier J, 1997, J PHOTOCH PHOTOBIO B, V38, P274, DOI 10.1016/S1011-1344(96)07464-7; MANUEL LJ, 1988, PLANT PHYSIOL, V88, P491, DOI 10.1104/pp.88.2.491; MARCUS Y, 1983, PLANT PHYSIOL, V71, P208, DOI 10.1104/pp.71.1.208; MORONEY JV, 1987, PLANT PHYSIOL, V83, P460, DOI 10.1104/pp.83.3.460; PALMQVIST K, 1994, PHYSIOL PLANTARUM, V90, P537; PALMQVIST K, 1988, PLANT PHYSIOL, V87, P437, DOI 10.1104/pp.87.2.437; Quinones MA, 1997, PHYSIOL PLANTARUM, V100, P45; RAMAZANOV Z, 1995, PLANTA, V195, P519, DOI 10.1007/BF00195709; RAMAZANOV Z, 1992, PHYSIOL PLANTARUM, V85, P121; RAMAZANOV Z, 1995, PLANTA, V197, P272, DOI 10.1007/BF00202647; RAMAZANOV ZM, 1986, SOV PLANT PHYSIOL+, V33, P659; RAMAZANOV ZM, 1988, SOV PLANT PHYSIOL+, V35, P340; RAMAZANOV ZM, 1984, SOV PLANT PHYSIOL+, V31, P344; ROTATORE C, 1991, CAN J BOT, V69, P1025, DOI 10.1139/b91-132; ROTATORE C, 1992, PLANTA, V188, P539, DOI 10.1007/BF00197046; Ruyters G., 1984, P283; Satoh A, 1996, PLANT CELL PHYSIOL, V37, P431, DOI 10.1093/oxfordjournals.pcp.a028964; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; Schmid R, 1998, PLANT CELL ENVIRON, V21, P523, DOI 10.1046/j.1365-3040.1998.00297.x; Schmidt FL, 1996, PSYCHOL METHODS, V1, P115, DOI 10.1037/1082-989X.1.2.115; SHIRAIWA Y, 1991, Japanese Journal of Phycology, V39, P355; SHIRAIWA Y, 1983, PLANT CELL PHYSIOL, V24, P919, DOI 10.1093/oxfordjournals.pcp.a076596; SHORT TW, 1994, ANNU REV PLANT PHYS, V45, P143, DOI 10.1146/annurev.pp.45.060194.001043; SPALDING MH, 1982, FEBS LETT, V145, P41, DOI 10.1016/0014-5793(82)81202-7; SPALDING MH, 1989, AQUAT BOT, V34, P181, DOI 10.1016/0304-3770(89)90056-9; SPALDING MH, 1989, PLANT PHYSIOL, V89, P133, DOI 10.1104/pp.89.1.133; SPENCER KG, 1983, PLANT CELL PHYSIOL, V24, P301, DOI 10.1093/pcp/24.2.301; SULTEMEYER DF, 1991, CAN J BOT, V69, P995, DOI 10.1139/b91-128; SULTEMEYER DF, 1989, PLANT PHYSIOL, V89, P1213, DOI 10.1104/pp.89.4.1213; THIELMANN J, 1990, PLANT PHYSIOL, V92, P622, DOI 10.1104/pp.92.3.622; UMINO Y, 1991, J PLANT PHYSIOL, V139, P41, DOI 10.1016/S0176-1617(11)80162-8; Villarejo A, 1996, PLANTA, V199, P481; WILLIAM JL, 1983, ANNU REV PLANT PHYS, V34, P71; WITT FG, 1995, PHOTOCHEM PHOTOBIOL, V61, P619, DOI 10.1111/j.1751-1097.1995.tb09878.x	55	8	8	0	13	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0022-0957			J EXP BOT	J. Exp. Bot.	APR	2000	51	345					807	815		10.1093/jexbot/51.345.807			9	Plant Sciences	Plant Sciences	309UF	WOS:000086787100017	10938873	Bronze			2021-04-07	
J	Delaroque, N; Wolf, S; Muller, DG; Knippers, R				Delaroque, N; Wolf, S; Muller, DG; Knippers, R			Characterization and immunolocalization of major structural proteins in the brown algal virus EsV-1	VIROLOGY			English	Article							ECTOCARPUS-SILICULOSUS; LAYER PROTEIN; COILED COILS; PHAEOPHYCEAE; SEQUENCE; GENOME; DNA; EXPRESSION; RING	The Ectocarpus siliculosus virus (EsV-1) is endemic in all populations of the cosmopolitan filamentous brown alga Ectocarpus siliculosus. EsV-1 has a large circular double-stranded DNA genome of about 320 kilobase pairs, and a complex virion structure with a central nucleoprotein core surrounded by several proteinaceous layers. To investigate the protein composition of the virion, we screened an expression library of EsV-1 with antibodies raised against purified detergent-disrupted viral particles. We isolated several clones encoding novel structural proteins and investigated two of them in detail. These clones encode viral proteins vp55 and vp74. Electron microscopy reveals that vp55 is most likely a component of the surface of the viral core, whereas vp74 may he part of an inner core structure. To initiate a genetic analysis, we sequenced regions of the EsV-1 genome encoding vp55 and vp74 and found several adjacent open reading frames with the potential to code for several interesting viral proteins including a putative calcium-binding protein, a collagen-like protein, and a RING finger protein. (C) 2000 Academic Press.	Univ Konstanz, Dept Biol, D-78457 Constance, Germany	Delaroque, N (corresponding author), Univ Konstanz, Dept Biol, D-78457 Constance, Germany.						ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; Awram P, 1998, J BACTERIOL, V180, P3062, DOI 10.1128/JB.180.12.3062-3069.1998; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; BRENDEL V, 1992, P NATL ACAD SCI USA, V89, P2002, DOI 10.1073/pnas.89.6.2002; COHEN C, 1994, SCIENCE, V263, P488, DOI 10.1126/science.8290957; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; GILCHRIST A, 1992, CAN J MICROBIOL, V38, P193, DOI 10.1139/m92-033; HARLOW E, 1988, ANTIBODIES LAB HDB; HEIZMANN CW, 1991, TRENDS BIOCHEM SCI, V16, P98, DOI 10.1016/0968-0004(91)90041-S; HOGER TH, 1991, EXP CELL RES, V197, P280, DOI 10.1016/0014-4827(91)90434-V; Jones DT, 1999, J MOL BIOL, V292, P195, DOI 10.1006/jmbi.1999.3091; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; KUHLMANN I, 1995, TIERSCHUTZBEAUFTRAGT, V2, P1; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; LANGBEIN L, 1993, DIFFERENTIATION, V55, P57, DOI 10.1111/j.1432-0436.1993.tb00033.x; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LUPAS A, 1991, SCIENCE, V252, P1162, DOI 10.1126/science.252.5009.1162; Maier I, 1998, EUR J PHYCOL, V33, P213, DOI 10.1017/S0967026298001747; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; PEARSON WR, 1988, P NATL ACAD SCI USA, V85, P2444, DOI 10.1073/pnas.85.8.2444; Pringle C R, 1998, Arch Virol, V143, P1449, DOI 10.1007/s007050050389; Provasoli L., 1968, CULTURES COLLECTIONS, P63; ROTH J, 1982, TECHNIQUES IMMUNOCYT, V1, P107; Sambrook J., 1989, MOL CLONING LAB MANU; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; Saurin AJ, 1996, TRENDS BIOCHEM SCI, V21, P208, DOI 10.1016/0968-0004(96)10036-0; Sedgwick SG, 1999, TRENDS BIOCHEM SCI, V24, P311, DOI 10.1016/S0968-0004(99)01426-7; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; STUDIER FW, 1986, J MOL BIOL, V189, P113, DOI 10.1016/0022-2836(86)90385-2; TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P4350, DOI 10.1073/pnas.76.9.4350; Van Etten JL, 1999, ANNU REV MICROBIOL, V53, P447, DOI 10.1146/annurev.micro.53.1.447; VENABLE JH, 1965, J CELL BIOL, V25, P407, DOI 10.1083/jcb.25.2.407; Wolf' S, 1998, PROTOPLASMA, V203, P153, DOI 10.1007/BF01279472	37	9	9	0	3	ACADEMIC PRESS INC	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA	0042-6822			VIROLOGY	Virology	MAR 30	2000	269	1					148	155		10.1006/viro.2000.0225			8	Virology	Virology	299XK	WOS:000086223600016	10725207				2021-04-07	
J	Muller, DG; Westermeier, R; Morales, J; Reina, GG; del Campo, E; Correa, JA; Rometsch, E				Muller, DG; Westermeier, R; Morales, J; Reina, GG; del Campo, E; Correa, JA; Rometsch, E			Massive prevalence of viral DNA in Ectocarpus (Phaeophyceae, Ectocarpales) from two habitats in the North Atlantic and South Pacific	BOTANICA MARINA			English	Article							SILICULOSUS PHAEOPHYCEAE; VIRUS GENOME; FASCICULATUS	Ectocarpus, a cosmopolitan genus of filamentous marine brown algae, contains two species, E. siliculosus and E. fasciculatus. Both species are subject to virus infections, which either destroy the host's sporangia or persist in a latent state without visible symptoms. We used PCR amplification of a viral gene fragment to monitor the infection status of Ectocarpus samples from Gran Canaria Island, North Atlantic, and southern Chile over 26 months. At both sites, we found persistently high levels of pathogen prevalence: 40-100% of the Ectocarpus specimens from Chile and 55-100% from Gran Canaria contained viral DNA. No evidence for seasonal variation could be detected. We conclude that vertical transmission of viral DNA through mitotic zoospores of the host is the key mechanism for the persistence of the pathogen. The PCR amplification characteristics of samples from Gran Canaria indicate that two different virus genotypes coexist in the same host population.	Univ Konstanz, Fak Biol, D-78457 Constance, Germany; Univ Austral Chile, Fac Pesquerias, Puerto Montt, Chile; Univ Las Palmas Gran Canaria, Inst Algol Aplicada, Telde, Gran Canaria, Spain; Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Santiago, Chile	Muller, DG (corresponding author), Univ Konstanz, Fak Biol, D-78457 Constance, Germany.						ASENSI AO, 1974, PHYSIS A, V86, P139; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; CLITHEROE SB, 1974, J ULTRA MOL STRUCT R, V49, P211, DOI 10.1016/S0022-5320(74)80032-8; Delaroque N, 1999, J GEN VIROL, V80, P1367, DOI 10.1099/0022-1317-80-6-1367; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; SAUVAGEAU C, 1896, J BOTANIQUE, V10, P140; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; SILVA PAUL C., 1957, MADRONO, V14, P41	12	10	11	0	4	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055			BOT MAR	Bot. Marina	MAR	2000	43	2					157	159		10.1515/BOT.2000.016			3	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	301FF	WOS:000086298000007					2021-04-07	
J	Diaz-Martin, MA; Espinoza-Avalos, J				Diaz-Martin, MA; Espinoza-Avalos, J			Distribution of brown seaweeds (Phaeophyta) in the Yucatan Peninsula, Mexico	BULLETIN OF MARINE SCIENCE			English	Article								Thirty five species of brown seaweeds (Phaeophyta) were found at 72 sites around the Yucatan peninsula. Dictyota menstrualis, Lobophora variegata, and Dictyota pulchella were the most common species. Seven new records were registered for the three states comprising the Yucatan peninsula: Hincksia sandriana in Quintana Roo; Hydroclathrus clathratus, Dictyopteris delicatula, Dictyota menstrualis, and D. mertensii in Campeche; and Hincksia mitchelliae. Ectocarpus rallsiae, and Sargassum cymosum in Yucatan. Twenty-eight species not found in this study but reported by other authors were included. Twenty one percent of the species were not distributed on both the Caribbean (Eastern Quintana Roo) and the Gulf of Mexico (Northwestern Quintana Roo, Yucatan and Campeche) coastlines of the peninsula. However, species richness was similar on both coastlines of the peninsula (58 vs 54 species, respectively). The northeastern tip of the peninsula (Cabo Catoche) is suggested as the coastal boundary between the Caribbean and the Gulf of Mexico phycoflora of the Yucatan peninsula.	ECOSUR, Chetumal 77000, Quintana Roo, Mexico	Diaz-Martin, MA (corresponding author), Univ Autonoma Baja California, Apdo Postal 453, Ensenada 22800, Baja California, Mexico.		Espinoza-Avalos, Julio/H-9189-2013	Espinoza-Avalos, Julio/0000-0001-5697-446X			Aguilar-Rosas L.E., 1992, ACTA BOT MEX, V19, P77; AGUILARROSAS LE, 1989, THESIS CICESE ENSENA; AGUILARROSAS LE, 1986, TAXNONMIA ECOLOGIA V; AGUILARROSAS LE, 1990, DIVERSIDAD BIOL RESE, P13; ALVAREZ JR, 1977, ENCICLOPEDIA MEXICO, V2; Arriaga-Cabrera L., 1998, REGIONES PRIORITARIA; Britton JC, 1989, SHORE ECOLOGY GULF M; Castillo Arenas Gerardo, 1995, Cryptogamie Algologie, V16, P115; CHAVE BML, 1980, ANAL ESCUELA NACL CI, V23, P45; COLLADOVIDES CML, 1995, ACTA BOT MEX, V31, P19; DIAZMARTIN MA, 1997, THESIS ITCH CHETUMAL; Dreckmann K. M., 1996, POLIBOTANICA, V3, P1; Earle SA, 1969, PHYCOLOGIA, V7, P71, DOI 10.2216/i0031-8884-7-2-71.1; EspinozaAvalos J, 1996, B MAR SCI, V59, P449; Fernandez-Equiarte A., 1993, CIENCIAS DESARROLLO, V18, P24; Garza-Barrientos M.A, 1975, MEM S LATIN OC BIOL, V2, P7; HUERTA L, 1966, CIENC MEX, V24, P193; Huerta-Muzquiz L, 1987, PHYTOLOGIA, V62, P22; HUERTAMUZQUIZ L, 1961, AN ESC NAC CIENC BIO, V10, P11; HUERTAMUZQUIZ L, 1958, ANALES ESCUELA NACL, V9, P115; HUERTAMUZQUIZ L, 1980, AN ESC NAC CIENC BIO, V23, P25; HUMM HJ, 1952, STAT U STUD, V7, P27; JOLY A., 1967, GENEROS ALGAS MARINA; KIM CS, 1964, THESIS DUKE U; Lanza-Espino G., 1991, OCEANOGRAFIA MARES M; LOPEZORNAT A, 1983, ESTUDIOS PRELIMINARE, P21; Mateo-Cid L.E., 1991, ACTA BOT MEX, V16, P57; Mendoza-Gonzalez A.C., 1992, ACTA BOT MEX, V19, P37; Merino M, 1997, J MARINE SYST, V13, P101, DOI 10.1016/S0924-7963(96)00123-6; Ortega Martha M., 1995, Anales del Instituto de Biologia Universidad Nacional Autonoma de Mexico Serie Botanica, V66, P1; PEDROCHE FF, 1990, ATLAS NACL MEXICO; SANCHEZALFARO F, 1977, THESIS UNAM MEXICO; TAYLOR WR, 1935, CARNEGIE I WASH PUBL, V461, P115; TAYLOR WR, 1928, CARNEGIE I WASHINGTO, V379, P1; TAYLOR WR, 1941, B SER FIELD MUS NAT, V20, P87; Taylor WR, 1960, MARINE ALGAE E TROPI; TORRESMEJIA E, 1991, THESIS UNAM; Woelkerling W., 1976, S FLORIDA BENTHIC MA; Wynne M.J., 1998, NOVA HEDWIGIA S, V116, P1	39	13	16	0	7	ROSENSTIEL SCH MAR ATMOS SCI	MIAMI	4600 RICKENBACKER CAUSEWAY, MIAMI, FL 33149 USA	0007-4977			B MAR SCI	Bull. Mar. Sci.	MAR	2000	66	2					279	289					11	Marine & Freshwater Biology; Oceanography	Marine & Freshwater Biology; Oceanography	295ZJ	WOS:000085998300001					2021-04-07	
J	Correa, JA; Ramirez, MA; de la Harpe, JP; Roman, D; Rivera, L				Correa, JA; Ramirez, MA; de la Harpe, JP; Roman, D; Rivera, L			Copper, copper mining effluents and grazing as potential determinants of algal abundance and diversity in northern Chile	ENVIRONMENTAL MONITORING AND ASSESSMENT			English	Article						algal diversity; coastal water; copper	ECTOCARPUS-SILICULOSUS; TRACE-METALS; TOLERANCE; CHLOROPHYTA; TOXICITY; KELP	We experimentally tested three alternative hypotheses to explain the low algal diversity and abundance in an intertidal zone receiving the effluents of the copper mine El Salvador in northern Chile. Our results demonstrated that algae were able to grow at the levels of dissolved copper detected in coastal waters of the area. During the assays, growth and regeneration in several red, green and brown adult algae and juvenile Lessonia nigrescens were normal at copper levels of 150 mu g L-1 or, in some cases, higher. We also found that the coastal sea water mixed with the effluent was not lethal to algae, although in some cases minor effects on growth were detected. These results indicate that today's low algal diversity and abundance can not be explained by the current copper levels in the area nor by the effect of the effluent. Exclusion of grazers, however, resulted in a fast colonization by various algal species. This, together with atypically high grazer density at the areas under the influence of the effluent, strongly suggests that herbivory, a factor not directly related to the mining operation, is likely to be responsible for the low algal diversity and abundance in the studied locality.	Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Santiago, Chile	Correa, JA (corresponding author), Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ecol, Casilla 114-D, Santiago, Chile.		Roman, Domingo/D-7841-2012				ANDERSON BS, 1990, MAR ECOL PROG SER, V68, P147, DOI 10.3354/meps068147; BRYAN GW, 1992, ENVIRON POLLUT, V76, P89, DOI 10.1016/0269-7491(92)90099-V; CASTILLA JC, 1983, MAR POLLUT BULL, V14, P459, DOI 10.1016/0025-326X(83)90046-2; Castilla JC, 1996, ENVIRON MONIT ASSESS, V40, P171, DOI 10.1007/BF00414390; CASTILLA JC, 1997, COPP REP INT M S AUS; CHUNG IK, 1986, MAR POLLUT BULL, V17, P213, DOI 10.1016/0025-326X(86)90603-X; Correa JA, 1996, ENVIRON MONIT ASSESS, V40, P41, DOI 10.1007/BF00395166; CORREA JA, 1988, J PHYCOL, V24, P528, DOI 10.1111/j.1529-8817.1988.tb04258.x; DAVIES DJA, 1985, SCI TOTAL ENVIRON, V46, P215, DOI 10.1016/0048-9697(85)90295-5; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; HARALDSSON C, 1988, MAR CHEM, V23, P417, DOI 10.1016/0304-4203(88)90108-9; HUNTER MD, 1992, ECOLOGY, V73, P724; Lobban CS, 1994, SEAWEED ECOLOGY PHYS; NRIAGU JO, 1979, NATURE, V279, P409, DOI 10.1038/279409a0; Paskoff R., 1990, J COASTAL RES, V6, P91; REED RH, 1983, J EXP MAR BIOL ECOL, V69, P85, DOI 10.1016/0022-0981(83)90173-9; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; SANTELICES B, 1990, HYDROBIOLOGIA, V192, P35, DOI 10.1007/BF00006226; Sokal R.R., 1981, BIOMETRY; STROMGREN T, 1979, J EXP MAR BIOL ECOL, V37, P153, DOI 10.1016/0022-0981(79)90092-3; STROMGREN T, 1980, MAR ENVIRON RES, V3, P5, DOI 10.1016/0141-1136(80)90032-X; THURSBY GB, 1995, FUNDAMENTALS AQUATIC, P257; 1997, CHILEAN MINING COMPE	23	23	25	1	11	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0167-6369			ENVIRON MONIT ASSESS	Environ. Monit. Assess.	MAR	2000	61	2					265	281					17	Environmental Sciences	Environmental Sciences & Ecology	280WJ	WOS:000085126500005					2021-04-07	
J	Kraan, S; Guiry, MD				Kraan, S; Guiry, MD			Sexual hybridization experiments and phylogenetic relationships as inferred from Rubisco spacer sequences in the genus Alaria (Phaeophyceae)	JOURNAL OF PHYCOLOGY			English	Article						Alaria esculenta; brown algae; chromosomes; cross experiments; hybridization; interspecific; intraspecific; phylogeny; Rubisco spacer	NUCLEOTIDE-SEQUENCES; KELP EVOLUTION; NORTH-ATLANTIC; ALGAE; TEMPERATURE; SYSTEMATICS; DIVERGENCE; RHODOPHYTA; ECTOCARPUS; LAMINARIA	Alaria (Alariaceae, Phaeophyceae) is a common genus of kelps generally found in the lower intertidal and shallow subtidal regions of rocky shores subject to strong wave exposure. Fourteen species are currently recognized, of which 11 are found in the cold-temperate North Pacific Ocean. Alaria esculenta (L,) Greville, the type species described from the North Atlantic, exhibits a range of biogeographically col-related morphotypes indicating the possibility of multiple species, subspecies, and/or hybrids. This has led to an unstable taxonomy, We compared five species from the Atlantic and Pacific, including six North Atlantic isolates of A. esculenta, Phylogenetic analyses based on Rubisco spacer sequences resulted in a well-resolved topology of these five species, but did not distinguish between the six biogeographic isolates of A. esculenta. Laboratory hybridization experiments among four A. esculenta isolates showed partial intrafertility, Among five tested Alaria species, interfertility as well as fertility barriers were encountered, inconsistent with reproductive isolation, The data reject both a biological and morphological species concept and support only a phylogenetic species concept for Alaria, demonstrating that morphological variation has evolved independently of molecular variation in the genes under investigation in the species of the genus Alaria.	Natl Univ Ireland Univ Coll Galway, Dept Bot, Martin Ryan Inst, Galway, Ireland	Kraan, S (corresponding author), Natl Univ Ireland Univ Coll Galway, Dept Bot, Martin Ryan Inst, Galway, Ireland.						ASSALI NE, 1991, PLANT MOL BIOL, V17, P853, DOI 10.1007/BF00037066; Bakker FT, 1995, THESIS U GRONINGEN N; BHATTACHARYA D, 1990, PLANT SYST EVOL, V170, P177, DOI 10.1007/BF00937702; BOLTON JJ, 1983, PHYCOLOGIA, V22, P133, DOI 10.2216/i0031-8884-22-2-133.1; BREEMAN AM, 1988, HELGOLANDER MEERESUN, V42, P199, DOI 10.1007/BF02366043; *CLIMAP, 1998, SEAS REC EARTHS SURF; Coyer JA, 1997, J PHYCOL, V33, P561, DOI 10.1111/j.0022-3646.1997.00561.x; DESTOMBE C, 1991, CURR GENET, V19, P395, DOI 10.1007/BF00309601; Doyle JJ., 1987, PHYTOCHEMISTRY, V19, P11, DOI DOI 10.2307/4119796; DRUEHL LD, 1992, PROG PHYCOL RES, V8, P47; DRUEHL LD, 1990, NATO ASI SERIES G, V22, P205; ESTES JA, 1988, PALEOBIOLOGY, V14, P19, DOI 10.1017/S0094837300011775; Felsenstein J., 1995, PHYLIP PHYLOGENY INF; GOFF LJ, 1994, J PHYCOL, V30, P521, DOI 10.1111/j.0022-3646.1994.00521.x; Grant V., 1981, PLANT SPECIATION; GREVILLE RK, 1830, ALGAE BRITANNICAE; Guiry M. D., 1991, SEAWEED RESOURCES EU; GUIRY MD, 1984, PHYCOLOGIA, V23, P357, DOI 10.2216/i0031-8884-23-3-357.1; GUIRY MD, 1992, PROGR PHYCOLOGICAL R, V8, P251; HARRISON RG, 1991, ANNU REV ECOL SYST, V22, P281, DOI 10.1146/annurev.ecolsys.22.1.281; HILLIS DM, 1992, J HERED, V83, P189, DOI 10.1093/oxfordjournals.jhered.a111190; HILLIS DM, 1987, ANNU REV ECOL SYST, V18, P23, DOI 10.1146/annurev.ecolsys.18.1.23; KAIN JM, 1987, HYDROBIOLOGIA, V151, P173, DOI 10.1007/BF00046124; KAIN JM, 1990, ECOMICALLY IMPORTANT; Kamiya M, 1998, J PHYCOL, V34, P361, DOI 10.1046/j.1529-8817.1998.340361.x; Kumar S, 1993, MEGA MOL EVOLUTIONAR; L?ning K., 1990, SEAWEEDS THEIR ENV B; LEWIS JL, 1986, AQUACULTURE, V57, P203; Lewis RJ, 1996, PHYCOLOGIA, V35, P19, DOI 10.2216/i0031-8884-35-1-19.1; LUNING K, 1978, PHYCOLOGIA, V17, P293, DOI 10.2216/i0031-8884-17-3-293.1; MAGGS CA, 1992, J PHYCOL, V28, P214, DOI 10.1111/j.0022-3646.1992.00214.x; Mai KS, 1996, AQUACULTURE, V139, P77, DOI 10.1016/0044-8486(95)01158-7; MANHART JR, 1992, J PHYCOL, V28, P730, DOI 10.1111/j.0022-3646.1992.00730.x; Medlin LK, 1995, NATO ADV SCI INST SE, V38, P133; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1985, PHYCOLOGIA, V24, P475, DOI 10.2216/i0031-8884-24-4-475.1; MUNDA IM, 1977, HELGOLAND WISS MEER, V29, P311, DOI 10.1007/BF01614267; NAKAHARA H, 1973, MAR BIOL, V18, P327; NICHOLAS KB, 1997, GENE DOC TOOL EDITIN; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Sambrook J., 1989, MOL CLONING LAB MANU; SAUNDERS GW, 1992, J PHYCOL, V28, P544, DOI 10.1111/j.0022-3646.1992.00544.x; Selivanova ON, 1997, BOT MAR, V40, P9, DOI 10.1515/botm.1997.40.1-6.9; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; SOUTH GR, 1970, HELGOLAND WISS MEER, V20, P216, DOI 10.1007/BF01609901; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STAM WT, 1988, HELGOLANDER MEERESUN, V42, P251, DOI 10.1007/BF02366045; SWOFFORD DL, 1993, PAUP PHYLOGENETIC AN; TAJIMA F, 1984, MOL BIOL EVOL, V1, P269; Tom Dieck Inka, 1993, Marine Ecology Progress Series, V100, P253; TOMDIECK I, 1993, MAR BIOL, V115, P151; TONDIECK I, 1992, PHYCOLOGIA, V31, P147; WIDDOWSON T B, 1971, Syesis, V4, P125; WIDDOWSON T B, 1971, Syesis, V4, P11; YARISH C, 1990, ECONOMICALLY IMPORTA; Yendo K, 1919, J COLL SCI TOKYO IMP, V153, P1	56	23	24	1	16	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044 USA	0022-3646			J PHYCOL	J. Phycol.	FEB	2000	36	1					190	198		10.1046/j.1529-8817.2000.99244.x			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	294NN	WOS:000085917500023					2021-04-07	
J	Terlizzi, A; Conte, E; Zupo, V; Mazzella, L				Terlizzi, A; Conte, E; Zupo, V; Mazzella, L			Biological succession on silicone fouling-release surfaces: Long-term exposure tests in the harbour of Ischia, Italy	BIOFOULING			English	Article						fouling; antifouling; silicone; easy-release coatings; Mediterranean Sea	MARINE EPIFAUNAL COMMUNITY; LARVAL SETTLEMENT; MICROSCALE ACCLIMATION; TROPICAL RHODOPHYTES; BOTRYLLUS-SCHLOSSERI; TRIBUTYLTIN; SUBSTRATUM; ECOPHYSIOLOGY; RECRUITMENT; TBT	A static test site was set up in the Harbour of Ischia (Gulf of Naples, Italy) to investigate the antifouling effectiveness of newly developed non-polluting coatings. Two-year exposure experiments were performed on sets of panels coated with silicone-based coatings, and results were compared both to sets of panels coated with toxic agents, and non-toxic epoxydic compounds. Abiotic factors, strength of adhesion of the temporal dynamics of succession of foulers were analyzed throughout the period of immersion. Brown algae constantly represented the "border point" between the early community, dominated by slime, micro- and macroalgae, and the late community, mainly represented by bryozoans and molluscs, as well as polychaetes, sponges and tunicates. Brown algae, such as Ectocarpus siliculosus tunicates (mainly Botryllus schlosseri) and polychaetes (Hydroides elegans, Pileolaria pseudomilitaris) were demonstrated to be key species, triggering the community and influencing its development. Light was the main abiotic factor discriminating the community on the two sides of panels exposed to different irradiances. The best performing coatings (silicone easy release coatings without additives) substantially influenced community structure, shifting it to the earliest stages of colonization. Silicone coatings proved to be unsuitable for colonization by organisms typical of mature communities, due to their low energy surfaces. The results of the present paper demonstrate that silicone coatings technology represents an alternative to the use of biocidal antifouling paints.	Staz Zool Anton Dohrn, Lab Ecol Benthos, I-80077 Ischia, NA, Italy		antonio.terlizzi@unile.it	Zupo, Valerio/A-5887-2011; Terlizzi, Antonio/B-6131-2015	Zupo, Valerio/0000-0001-9766-8784; Terlizzi, Antonio/0000-0001-5968-4548			Alberte RS, 1992, BIOFOULING, V6, P91, DOI 10.1080/08927019209386214; ALZIEU C, 1991, MAR ENVIRON RES, V32, P7, DOI 10.1016/0141-1136(91)90029-8; ANDERSON MJ, 1994, J EXP MAR BIOL ECOL, V184, P217, DOI 10.1016/0022-0981(94)90006-X; ANGER K, 1978, HELGOL WISS MEERESUN, V31, P475; *ASTM, 1994, 561894 ASTM D; *ASTM, 1987, 362378AASTM D; Baier RE, 1992, BIOFOULING, V6, P165, DOI 10.1080/08927019209386220; Beach KS, 1997, CORAL REEFS, V16, P21, DOI 10.1007/s003380050055; Beach KS, 1996, J PHYCOL, V32, P701, DOI 10.1111/j.0022-3646.1996.00701.x; Beach KS, 1996, J PHYCOL, V32, P710, DOI 10.1111/j.0022-3646.1996.00710.x; BEACH KS, 1995, MAR ECOL PROG SER, V125, P229, DOI 10.3354/meps125229; BEAUMONT AR, 1984, MAR POLLUT BULL, V15, P402, DOI 10.1016/0025-326X(84)90256-X; BECKA A, 1984, BIOTECHNOL BIOENG, V26, P1245, DOI 10.1002/bit.260261015; BECKER K, 1993, MAR BIOL, V117, P301, DOI 10.1007/BF00345675; BRUNETTI R, 1974, Bollettino di Zoologia, V41, P225; CALLOW ME, 1994, INT BIODETER BIODEGR, V34, P333, DOI 10.1016/0964-8305(94)90092-2; CARWILE AH, 1989, THESIS UCLA LOS ANGE; CHIMENZGUSSO C, 1973, ATT 5 C NAZ SOC IT B, P66; CINELLI F, 1976, ARCH OCEANOGR LIMNOL, V18, P169; CONNELL JH, 1977, AM NAT, V111, P1119, DOI 10.1086/283241; Costlow JDC, 1984, MARINE BIODETERIORAT; CRISP DJ, 1960, NATURE, V185, P119, DOI 10.1038/185119a0; Dahl B, 1996, MAR POLLUT BULL, V32, P342, DOI 10.1016/0025-326X(96)84828-4; ELLIS DV, 1991, MAR POLLUT BULL, V22, P8, DOI 10.1016/0025-326X(91)90437-W; Evans L.V., 1988, ALGAE HUMAN AFFAIRS, P433; Evans SM, 1999, MAR POLLUT BULL, V38, P629, DOI 10.1016/S0025-326X(99)00040-5; FRESI E, 1981, Memorie di Biologia Marina e di Oceanografia, V11, P207; FRONTIER S, 1974, THESIS U MARSEILLE; GAINES S, 1985, P NATL ACAD SCI USA, V82, P3707, DOI 10.1073/pnas.82.11.3707; GIBBS PE, 1991, COMP BIOCHEM PHYS C, V100, P231, DOI 10.1016/0742-8413(91)90159-Q; GROSBERG RK, 1988, EVOLUTION, V42, P900, DOI 10.1111/j.1558-5646.1988.tb02510.x; Haderlie E.C, 1984, MARINE BIODETERIORAT, P163, DOI DOI 10.1007/978-1-4615-9720-9_21; HADFIELD MG, 1994, RECENT DEVELOPMENTS IN BIOFOULING CONTROL, P65; HARLIN MM, 1977, MAR BIOL, V40, P33, DOI 10.1007/BF00390625; HARMS J, 1983, HELGOLANDER MEERESUN, V36, P137, DOI 10.1007/BF01983853; HENLEY WJ, 1992, OECOLOGIA, V89, P516, DOI 10.1007/BF00317158; HOLMSTROM C, 1994, BIOFOULING, V8, P147, DOI 10.1080/08927019409378269; JAMES RJ, 1994, J EXP MAR BIOL ECOL, V181, P105, DOI 10.1016/0022-0981(94)90107-4; KIRCHMAN D, 1982, MAR BIOL LETT, V3, P131; Lindner E, 1992, BIOFOULING, V6, P193, DOI 10.1080/08927019209386222; MAZZELLA L, 1979, DIATOMS COMM INT MER, V25, P153; MCGUINNESS KA, 1989, MAR ECOL PROG SER, V52, P201, DOI 10.3354/meps052201; MICHAEL T, 1995, MAR ECOL PROG SER, V119, P229, DOI 10.3354/meps119229; Oshurkov V.V., 1992, Biofouling, V6, P1; PAHLWOSTL P, 1995, DYNAMIC NATURE ECOSY; PAUL JD, 1986, AQUACULTURE, V54, P191, DOI 10.1016/0044-8486(86)90328-5; Pielou E. C., 1984, INTERPRETATION ECOLO; Relini G, 1993, OEBALIA S, V19, P103; Richmond M.D., 1991, Biofouling, V3, P151; Rinkevich B, 1998, MAR ECOL-P S Z N I, V19, P129, DOI 10.1111/j.1439-0485.1998.tb00458.x; Rittschof D, 1992, BIOFOULING, V6, P181, DOI 10.1080/08927019209386221; RITTSCHOF D, 1984, J EXP MAR BIOL ECOL, V82, P131, DOI 10.1016/0022-0981(84)90099-6; RUIZ JM, 1995, MAR BIOL, V124, P119, DOI 10.1007/BF00349153; RUSS GR, 1980, J EXP MAR BIOL ECOL, V42, P55, DOI 10.1016/0022-0981(80)90166-5; RUSS GR, 1982, OECOLOGIA, V53, P12, DOI 10.1007/BF00377130; Sabbadin A., 1972, P327; Scheltema R.S., 1974, Thalassia Jugosl, V10, P263; Schmidt G.H., 1989, Biofouling, V1, P223; SCHMIDT GH, 1986, MAR ECOL PROG SER, V31, P101, DOI 10.3354/meps031101; SCIPIONE MB, 1982, ATT CONV UN SOTT BIO, P107; SHOENER A, 1978, ECOLOGY, V59, P367; SMITH CM, 1994, MAR BIOL, V118, P511, DOI 10.1007/BF00350308; SOUTHERLAND JP, 1978, ECOLOLGY, V59, P257; SUNBERG DC, 1997, NAV RES REV, V49, P51; Swain GW, 1992, BIOFOULING, V6, P105, DOI 10.1080/08927019209386216; SWAIN GW, 1995, GUIDELINES STAND REP; TERLIZZI A, 2000, IN PRESS ITAL J ZOOL; TODD CD, 1994, J EXP MAR BIOL ECOL, V181, P159, DOI 10.1016/0022-0981(94)90126-0; Underwood A.J., 1984, P151; UNDERWOOD AJ, 1994, J MAR BIOL ASSOC UK, V74, P563, DOI 10.1017/S0025315400047676; Walters LJ, 1996, MAR BIOL, V126, P383, DOI 10.1007/BF00354620; WOOSLACOTT RM, 1984, MARINE BIODETERIORAT, P149; YOUNG GA, 1983, J MAR BIOL ASSOC UK, V63, P653, DOI 10.1017/S002531540007096X	73	58	60	0	18	HARWOOD ACAD PUBL GMBH	READING	C/O STBS LTD, PO BOX 90, READING RG1 8JL, BERKS, ENGLAND	0892-7014			BIOFOULING	Biofouling		2000	15	4					327	342		10.1080/08927010009386322			16	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	355RX	WOS:000089400300008					2021-04-07	
J	Kupper, H; Setlik, I; Trtilek, M; Nedbal, L				Kupper, H; Setlik, I; Trtilek, M; Nedbal, L			A microscope for two-dimensional measurements of in vivo chlorophyll fluorescence kinetics using pulsed measuring radiation, continuous actinic radiation, and saturating flashes	PHOTOSYNTHETICA			English	Article; Proceedings Paper	3rd Regional Photosynthesis Workshop on the Chlorophyll Fluorescence Imaging and its Application in Plant Science and Technology	FEB 06-11, 2000	LIPNO, CZECH REPUBLIC	Czech-German Future Fund, Photon Syst Instruments, Hansatech Instruments		Ectocarpus; Elodea; fluorescence imaging; fluorescence quenching analysis; Hibiscus; photosynthetic heterogeneity; photosystem 2; Scenedesmus; topography of fluorescence induction	A-FLUORESCENCE; PLANT STRESS; LEAVES; PHOTOSYNTHESIS; TOPOGRAPHY; IMAGES; DAMAGE	Transients of chlorophyll fluorescence in photosynthetic objects are often measured using short pulses of exciting radiation, which has recently been employed to capture kinetic images of fluorescence at the macroscopic level. Here we describe an instrument introducing this principle to recording of two dimensional fluorescence transients in microscopic objects. A modified fluorescence microscope is equipped with a CCD camera intensified by a micro-channel plate image amplifier. The microscopic field is irradiated simultaneously by three types of radiation: actinic radiation, saturating flashes, and pulsed measuring radiation. The measuring pulses are generated by a light-emitting diode and their duration is between 10 to 250 mus The detection of fluorescence images (300x400 pixels, 8 bit) has a maximum time resolution of 40 ms and is gated in synchrony with the exciting pulses. This allows measuring on a background of a continuous actinic radiation up to irradiance that can elicit the maximal fluorescence yield (F-M). On the other hand, the integral irradiance of the objects by the measuring radiation is very low, e.g., 0.08 mu mol m(-2) s(-1) at 0.5 mum spatial resolution and 0.006 mu mol m(-2) s(-1) at 4 mum spatial resolution. This allows a reliable recording of F-0 even in very short time intervals (e.g., 5x80 ms). The software yields fluorescence kinetic curves for objects in user-selected areas as well as complete false-colour maps of the essential fluorescence kinetics parameters (F-M, F-0, F-V, F-V/F-M, etc.) showing a two-dimensional distribution of their values. Several examples demonstrate that records of fluorescence kinetics can be obtained with a reasonable signal-to-noise ratio with all standard microscope objectives and with object sizes reaching from segments of leaf tissue to individual algal cells or chloroplasts.	Univ Konstanz, Fac Sci, Dept Biol, D-78457 Constance, Germany; Acad Sci Czech Republ, Dept Autotroph Microorganisms, Inst Microbiol, CZ-37981 Trebon, Czech Republic; Univ S Bohemia, Fac Biol, CZ-37005 Ceske Budejovice, Czech Republic; Photon Syst Instruments Ltd, CZ-62100 Brno, Czech Republic; Acad Sci Czech Republ, Inst Landscape Ecol, Photosynth Res Ctr, CZ-37333 Nove Hrady, Czech Republic	Setlik, I (corresponding author), Univ Konstanz, Fac Sci, Dept Biol, D-78457 Constance, Germany.	Setlik@alga.cz	Kupper, Hendrik/J-5152-2012	Kupper, Hendrik/0000-0003-0712-7023			BILGER W, 1990, PHOTOSYNTH RES, V25, P161, DOI 10.1007/BF00033158; Bowyer WJ, 1998, SPECTROSCOPY, V13, P36; Buschmann C, 1998, J PLANT PHYSIOL, V152, P297, DOI 10.1016/S0176-1617(98)80144-2; CARDON ZG, 1994, PLANT CELL ENVIRON, V17, P995, DOI 10.1111/j.1365-3040.1994.tb02033.x; DALEY PF, 1989, PLANT PHYSIOL, V90, P1233, DOI 10.1104/pp.90.4.1233; DALEY PF, 1995, CAN J PLANT PATHOL, V17, P167, DOI 10.1080/07060669509500708; DAU H, 1994, PHOTOCHEM PHOTOBIOL, V60, P1; FENTON JM, 1990, PHOTOSYNTH RES, V26, P59, DOI 10.1007/BF00048977; GOVINDJEE, 1995, AUST J PLANT PHYSIOL, V22, P131, DOI 10.1071/PP9950131; Jensen M, 1997, SYMBIOSIS, V23, P183; KRAUSE GH, 1991, ANNU REV PLANT PHYS, V42, P313, DOI 10.1146/annurev.pp.42.060191.001525; Lichtenthaler HK, 1996, J PLANT PHYSIOL, V148, P599, DOI 10.1016/S0176-1617(96)80081-2; Lootens P, 2000, PHOTOSYNTHETICA, V38, P53, DOI 10.1023/A:1026791823551; Meyer S, 1998, PLANT PHYSIOL, V116, P947, DOI 10.1104/pp.116.3.947; NEDBAL L, 2001, IN PRESS PHOTOSYNTH; OMASA K, 1987, PLANT PHYSIOL, V84, P748, DOI 10.1104/pp.84.3.748; Osmond B, 1999, AUST J PLANT PHYSIOL, V26, P717, DOI 10.1071/PP99077; Oxborough K, 1997, PLANT CELL ENVIRON, V20, P1473, DOI 10.1046/j.1365-3040.1997.d01-42.x; Oxborough K, 1997, PHOTOSYNTH RES, V54, P135, DOI 10.1023/A:1005936823310; ROLFE SA, 1995, NEW PHYTOL, V131, P69, DOI 10.1111/j.1469-8137.1995.tb03056.x; SCHREIBER U, 1993, PHOTOSYNTH RES, V36, P65, DOI 10.1007/BF00018076; SCHREIBER U, 1986, PHOTOSYNTH RES, V10, P51, DOI 10.1007/BF00024185; SCHREIBER U, 1995, AUST J PLANT PHYSIOL, V22, P209, DOI 10.1071/PP9950209; Schreiber U, 1998, PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V, P4253; Siebke K, 1995, PHOTOSYNTH RES, V45, P225, DOI 10.1007/BF00015563; VANKOOTEN O, 1990, PHOTOSYNTH RES, V25, P147, DOI 10.1007/BF00033156	26	42	44	0	15	ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY	6 PRAGUE	NA KARLOVCE 1A,, 6 PRAGUE, 160 00, CZECH REPUBLIC	0300-3604	1573-9058		PHOTOSYNTHETICA	Photosynthetica		2000	38	4					553	570		10.1023/A:1012461407557			18	Plant Sciences	Plant Sciences	454PP	WOS:000169981200010					2021-04-07	
J	Morton, SL; Leighfield, TA; Haynes, BL; Petitpain, DL; Busman, MA; Moeller, PDR; Bean, L; Mcgowan, J; Hurst, JW; Van Dolah, FM				Morton, SL; Leighfield, TA; Haynes, BL; Petitpain, DL; Busman, MA; Moeller, PDR; Bean, L; Mcgowan, J; Hurst, JW; Van Dolah, FM			Evidence of diarrhetic shellfish poisoning along the coast of Maine	JOURNAL OF SHELLFISH RESEARCH			English	Article							OKADAIC ACID; TOXIN	Following the occurrence of several unexplained incidents of shellfish-related gastroenteritis, field studies were conducted to determine if diarrhetic shellfish poisoning (DSP) toxins are present in Maine coastal waters. A protein phosphatase inhibition assay for DSP toxins revealed the presence of low levels of okadaic acid-like activity in blue mussels (Mytilus edulis) at sampling sites in the Frenchman Bay-Eastern Bay region. All other sires along the Maine coast were negative. Phytoplankton populations from this area were dominated by Dinophysis norvegica, a known toxic species. Two additional known toxic species of Dinophysis were also found: Dinophysis acuminata and D. rotunda. However, all plankton samples were negative for okadaic acid-like activity. Examination of the epiphytic communities from areas where mussels showed okadaic acid-like activity revealed the presence of the toxic dinoflagellate Prorocentrum lima in association with the brown alga, Ectocarpus sp. Epiphytic samples rich in P. lima were active in the phosphatase inhibition assay; Subsequent analysis of these samples using LC-MS/MS identified the presence of dinophysis toxin-l (DTX-1). Empty P. lima thecae identified in the digestive tract of mussels from these areas indicate that P. lima is consumed by mussels. This is the first confirmation of P. lima in northern United States coastal waters and identifies DSP as a potential public health issue.	Natl Ocean Serv, Marine Biotoxins Program, Ctr Coastal Environm Hlth & Biomol Res, NOAA, Charleston, SC 29412 USA; Maine Dept Marine Resources, W Boothbay Harbor, ME 04575 USA; NIEHS, Marine & Freshwater Biomed Sci Ctr, Mt Desert Isl Biol Lab, Salsbury Cove, ME 04672 USA	Morton, SL (corresponding author), Natl Ocean Serv, Marine Biotoxins Program, Ctr Coastal Environm Hlth & Biomol Res, NOAA, Charleston, SC 29412 USA.			Busman, Mark/0000-0001-9750-064X			Bomber J.W., 1985, THESIS FLORIDA I TEC; Carlson R.D., 1985, P171; FERNANDEZ ML, 1996, TOXICON, V34, P351; Kat M., 1985, P73; KUMAGAI M, 1986, AGR BIOL CHEM TOKYO, V50, P2853, DOI 10.1080/00021369.1986.10867817; Lawrence J.E., 1998, HARMFUL ALGAE, P78; MARR JC, 1992, J APPL PHYCOL, V4, P17, DOI 10.1007/BF00003956; McLachlan J. L., 1994, Natural Toxins, V2, P263, DOI 10.1002/nt.2620020504; MORTON SL, 1994, J EXP MAR BIOL ECOL, V178, P67, DOI 10.1016/0022-0981(94)90225-9; MORTON SL, 1990, TOXIC MARINE PHYTOPLANKTON, P201; Morton SL, 1997, B MAR SCI, V61, P899; QUILLIAM MA, 1990, P 2 CAN WORKSH HARMF, P18; SHUMWAY S E, 1988, Journal of Shellfish Research, V7, P643; Suzuki T, 1999, TOXICON, V37, P187, DOI 10.1016/S0041-0101(98)00182-2; Tubaro A, 1996, TOXICON, V34, P743, DOI 10.1016/0041-0101(96)00027-X; Wright Jeffrey L.C., 1998, NATO ASI Series Series G Ecological Sciences, V41, P427; YASUMOTO T, 1978, B JPN SOC SCI FISH, V44, P1249	17	24	25	0	8	NATL SHELLFISHERIES ASSOC	SOUTHAMPTON	C/O DR. SANDRA E. SHUMWAY, NATURAL SCIENCE DIVISION, SOUTHAMPTON COLLEGE, SOUTHAMPTON, NY 11968 USA	0730-8000			J SHELLFISH RES	J. Shellfish Res.	DEC	1999	18	2					681	686					6	Fisheries; Marine & Freshwater Biology	Fisheries; Marine & Freshwater Biology	276YM	WOS:000084906000043					2021-04-07	
J	Kogame, K; Horiguchi, T; Masuda, M				Kogame, K; Horiguchi, T; Masuda, M			Phylogeny of the order Scytosiphonales (Phaeophyceae) based on DNA sequences of rbcL, partial rbcS, and partial LSU nrDNA	PHYCOLOGIA			English	Article							ECTOCARPALES SENSU-LATO; LARGE RIBOSOMAL-RNA; CHLOROPHYTA; PLASTIDS; POSITION; GENE	A molecular phylogenetic study of the order Scytosiphonales (Phaeophyceae) was carried out using DNA sequences of rbcL, partial r-bcS, and partial large subunit (LSU) nrDNA (domains D1 and D2) that were determined in 14 species. Ectocarpus siliculosus (Dillwyn) Lyngbye was adopted as an outgroup taxon. Phylogenetic trees inferred in maximum-parsimony and neighbor-joining analyses were almost identical to each other and showed two large clades. A monophyletic group distributed in warm-temperate to tropical regions includes Chnoospora implexa J. Agardh, Rosenvingea intricata (J. Agardh) Borgesen, Hydroclatrus clathratus (C. Agardh) Howe, and Colpomenia sinuosa (Mertens ex Roth) Derbes et Solier. The second clade includes Colpomenia bullosa (Saunders) Yamada, Colpomenia phaeodactyla Wynne et J. Norris, four species of Scytosiphon, and three species of Petalonia, which are mostly distributed in cold-temperate regions. Colpomenia peregrina (Sauvageau) Hamel showed an ambiguous position between the two large clades. Within the second large clade, a subclade of Petalonia fascia (O. F. Muller) Kuntze, P. binghamiae (J. Agardh) Vinogradova, and Scytosiphon tenellus Kogame and another subclade of Scytosiphon gracilis Kogame and Petalonia zosterifolia (Reinke) Kuntze were supported by high bootstrap values. These results seriously question autonomy of the Chnoosporaceae and monophyly of the genera Colpomenia, Scytosiphon, and Petalonia. Morphological characters of the prostrate sporophytes, such as thallus structure and presence or absence of plurilocular zoidangia, were congruent with the molecular phylogeny. These features are likely to be more important taxonomic criteria at the generic or family level in the Scytosiphonales than is the morphology of the erect gametophytes.	Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan	Kogame, K (corresponding author), Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan.		Horiguchi, Takeo/D-7612-2012	Horiguchi, Takeo/0000-0002-6118-8460			ABBOTT LA, 1976, MARINE ALGAE CALIFOR; AGARWAL RP, 1996, COMPUT MATH APPL, V32, P1; Bailey JC, 1997, EUR J PHYCOL, V32, P343; BAKKER FT, 1995, EUR J PHYCOL, V30, P197, DOI 10.1080/09670269500650981; Bold H. C., 1985, INTRO ALGAE STRUCTUR; BOrgesen F., 1939, DANISH SCI INVESTI 1, P47; Burkhardt E, 1998, J PHYCOL, V34, P682, DOI 10.1046/j.1529-8817.1998.340682.x; CLAYTON MN, 1978, MAR BIOL, V47, P349, DOI 10.1007/BF00388926; CLAYTON MN, 1981, P 8 INT SEAW S, P67; COLE K, 1970, Phycologia, V9, P275, DOI 10.2216/i0031-8884-9-3-275.1; DANGEARD P, 1963, BOTANISTE, V46, P5; DELEPINE R, 1978, Revue Algologique, V13, P43; Feldmann J, 1949, MEMOIRES HORS SERIE, V2, P103; Felsenstein J., 1993, PHYLIP PHYLOGENY INF; Fletcher RL, 1987, SEAWEEDS BRIT ISLES, VIII; Fritsch FE, 1945, STRUCTURE REPROD ALG; GARRIGA G, 1984, CELL, V36, P623, DOI 10.1016/0092-8674(84)90342-8; HOLMQUIST R, 1983, J MOL EVOL, V19, P134, DOI 10.1007/BF02300751; Horiguchi T, 1998, PHYCOLOGIA, V37, P237, DOI 10.2216/i0031-8884-37-4-237.1; HSIAO SIC, 1969, CAN J BOTANY, V47, P1611, DOI 10.1139/b69-231; KAWAI H, 1995, J PHYCOL, V31, P306, DOI 10.1111/j.0022-3646.1995.00306.x; KOGAME K, 1996, PHYCOLOGICAL RES, V44, P86; Kogame Kazuhiro, 1998, Phycological Research, V46, P39, DOI 10.1111/j.1440-1835.1998.tb00095.x; Kogame Kazuhiro, 1993, Japanese Journal of Phycology, V41, P29; Kogame Kazuhiro, 1997, Phycological Research, V45, P227, DOI 10.1111/j.1440-1835.1997.tb00081.x; Kogame K, 1997, PHYCOLOGIA, V36, P389, DOI 10.2216/i0031-8884-36-5-389.1; Kogame K, 1997, PHYCOLOGIA, V36, P337, DOI 10.2216/i0031-8884-36-5-337.1; Kumar S, 1993, MEGA MOL EVOLUTIONAR; L?ning K., 1990, SEAWEEDS THEIR ENV B; LUND S, 1966, PHYCOLOGIA, V6, P67; MICHOT B, 1984, NUCLEIC ACIDS RES, V12, P4259, DOI 10.1093/nar/12.10.4259; NAKAMURA Y, 1975, Scientific Papers of the Institute of Algological Research Faculty of Science Hokkaido University, V6, P57; Nakamura Y., 1977, B JAP SOC PHYCOL S, V25, P203; PAKKER H, 1994, J PHYCOL, V30, P777, DOI 10.1111/j.0022-3646.1994.00777.x; Peters AF, 1997, J PHYCOL, V33, P294, DOI 10.1111/j.0022-3646.1997.00294.x; Peters AF, 1998, PHYCOLOGIA, V37, P114, DOI 10.2216/i0031-8884-37-2-114.1; Peters AF, 1998, PHYCOLOGIA, V37, P106, DOI 10.2216/i0031-8884-37-2-106.1; ROSENVINGE L. KOLDERUP, 1947, K DANSKE VIDENSK SELSKAB BIOL SKRIFT, V4, P1; Rousseau F, 1997, PHYCOLOGIA, V36, P438, DOI 10.2216/i0031-8884-36-6-438.1; Rousseau F, 1999, CRYPTOGAMIE ALGOL, V20, P5, DOI 10.1016/S0181-1568(99)80002-6; Setchell WA, 1925, U CALIF PUBL BOT, V8, P383; Siemer BL, 1998, J PHYCOL, V34, P1038, DOI 10.1046/j.1529-8817.1998.341038.x; SWOFFORD DL, 1993, PAUP PHYLOGENETIC AN; Tan IH, 1996, J PHYCOL, V32, P112, DOI 10.1111/j.0022-3646.1996.00112.x; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; VALENTIN K, 1990, PLANT MOL BIOL, V15, P575, DOI 10.1007/BF00017832; Vinogradova K. L., 1973, NOVITATES SYSTEMATIC, V10, P28; Womersley H.B.S., 1987, MARINE BENTHIC FLORA; Wynne M, 1969, U CALIF PUBL BOT, V50, P1; Wynne M. J., 1976, SMITHSONIAN CONTRIBU, V35, P1; Wynne M. J., 1972, CONTRIBUTIONS SYSTEM, P133; YAMOTO A, 1997, THESIS HOKKAIDO U GR; ZHU H, 1993, NUCLEIC ACIDS RES, V21, P5279, DOI 10.1093/nar/21.22.5279	53	85	91	0	15	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	NOV	1999	38	6					496	502		10.2216/i0031-8884-38-6-496.1			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	269DV	WOS:000084462000002					2021-04-07	
J	Kreimer, G				Kreimer, G			Reflective properties of different eyespot types in dinoflagellates	PROTIST			English	Article							LASER SCANNING MICROSCOPY; CHLAMYDOMONAS-REINHARDTII; ECTOCARPUS-SILICULOSUS; MARINE DINOFLAGELLATE; ULTRASTRUCTURE; ALGAE; DINOPHYCEAE; PHOTOTAXIS; APPARATUS; MUTANT	The reflective properties of different types of dinoflagellate eyespots were investigated using confocal laser scanning microscopy in the epireflection contrast mode. Although the eyespots studied differed with respect to localization (cytosol or plastid) and organization of the globule layer(s), all types effectively absorbed and reflected blue-green laser light (principal lines of 488/514 nm). The relative orientation of the eyespot surface towards the light source strongly influenced the reflective properties. Maximal reflection occurred when the eyespot surface was approximately perpendicular to the light source and rapidly decreased at increasing angles of light incidence. Horizontal and vertical optical sectioning of live and fixed cells resolved differences in the reflection patterns. Focusing of reflected light on the basal portion of the longitudinal flagellum was observed for the cytosolic eyespot of Glenodinium sp. and the triple membrane-bounded eyespot of Peridinium foliaceum, presumably a vestige of a host plastid. This flagellum is thought to be mainly involved in mediating orientational movement responses. In contrast, the reflection patterns obtained from the eyespot of Woloszynskia pascheri, which represents the third and most commonly observed dinoflagellate eyespot type within a plastid, point to only minor focusing. Reflection signals could be followed a considerable distance into the sulcus in all cases, indicating that in dinoflagellate eyespots, irrespective of the presumed receptor location (plasma membrane overlying the eyespot and/or the basal part of the longitudinal flagellum), back reflection of non-absorbed light can enhance the excitation probability of the photoreceptor(s). Such a combined reflection/absorption screen allows maximal contrast modulation and will, in conjunction with the specialized geometry of the dinoflagellate eyespots, increase the directionality of these eyespot apparatuses considerably.	Univ Cologne, Inst Bot, Lehrstuhl 1, D-50931 Cologne, Germany	Kreimer, G (corresponding author), Univ Cologne, Inst Bot, Lehrstuhl 1, Gyrhofstr 15, D-50931 Cologne, Germany.	Georg.Kreimer@uni-koeln.de					Calado AJ, 1998, J PHYCOL, V34, P536, DOI 10.1046/j.1529-8817.1998.340536.x; COLEMAN AW, 1988, J PHYCOL, V24, P118; CRAWFORD RM, 1974, NOVA HEDWIGIA, V22, P699; CRESCITELLI F, 1992, VISION RES, V32, P1593, DOI 10.1016/0042-6989(92)90152-9; Dodge J.D., 1984, Biosystems, V16, P259; DODGE JD, 1969, J CELL SCI, V5, P479; DODGE JD, 1973, EYESPOT FINE STRUCTU, P125; EGGERSDORFER B, 1991, ACTA PROTOZOOL, V30, P63; FENSOME RA, 1993, MICROPALEONTOLOGY SP, V7; FOSTER KW, 1980, MICROBIOL REV, V44, P572, DOI 10.1128/MMBR.44.4.572-630.1980; FRANCIS D, 1967, J EXP BIOL, V47, P495; Goldstein S.F., 1992, P99; GREUET C, 1978, CYTOBIOLOGIE, V17, P114; GREUET C, 1987, BIOL DINOFLAGELLATES, P119; GRUNG M, 1994, PLANTA, V193, P38; HALLDAL PER, 1958, PHYSIOL PLANTARUM, V11, P118; HAND WG, 1970, J EXP ZOOL, V174, P33, DOI 10.1002/jez.1401740104; HAND WG, 1975, J PROTOZOOL, V22, P494, DOI 10.1111/j.1550-7408.1975.tb05217.x; HORIGUCHI T, 1994, PROTOPLASMA, V179, P142, DOI 10.1007/BF01403952; HORIGUCHI T, 1991, BOT MAR, V34, P123, DOI 10.1515/botm.1991.34.2.123; HORIGUCHI T, 1994, EUR J PHYCOL, V29, P237, DOI 10.1080/09670269400650691; Horiguchi Takeo, 1999, Phycological Research, V47, P101, DOI 10.1111/j.1440-1835.1999.tb00290.x; Kamykowski D, 1998, J PLANKTON RES, V20, P1781, DOI 10.1093/plankt/20.9.1781; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KAWAI H, 1999, FLAGELLATES UNITY DI; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KREIMER G, 1994, INT REV CYTOL, V148, P229, DOI 10.1016/S0074-7696(08)62409-2; KREIMER G, 1990, EUR J CELL BIOL, V53, P101; KREIMER G, 1992, PLANTA, V188, P513, DOI 10.1007/BF00197043; KREIMER G, 1999, COMPREHENSIVE SERIES; Levandowsky M, 1987, BIOL DINOFLAGELLATES, V21, P360; MCFADDEN GI, 1986, PHYCOLOGIA, V25, P551, DOI 10.2216/i0031-8884-25-4-551.1; Melkonian M., 1984, Progress phycol. Res., V3, P193; MORELLAURENS NML, 1983, PHOTOCHEM PHOTOBIOL, V37, P189, DOI 10.1111/j.1751-1097.1983.tb04457.x; REIZE IB, 1989, BOT ACTA, V102, P145, DOI 10.1111/j.1438-8677.1989.tb00083.x; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; Santos LMA, 1996, PHYCOLOGIA, V35, P299, DOI 10.2216/i0031-8884-35-4-299.1; Saunders GW, 1997, PLANT SYST EVOL, P237; Schaller K, 1997, BIOPHYS J, V73, P1573, DOI 10.1016/S0006-3495(97)78189-X; SCHLOSSER UG, 1994, BOT ACTA, V107, P113, DOI 10.1111/j.1438-8677.1994.tb00784.x; SCHNEPF E, 1972, PROTOPLASMA, V74, P411, DOI 10.1007/BF01281959; SPECTOR DL, DINOFLAGELLATES; TOMAS RN, 1973, J PHYCOL, V9, P304; WITHERS NW, 1978, PLANT PHYSIOL, V62, P36, DOI 10.1104/pp.62.1.36	45	14	14	0	10	ELSEVIER GMBH, URBAN & FISCHER VERLAG	JENA	OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY	1434-4610			PROTIST	Protist	OCT	1999	150	3					311	323		10.1016/S1434-4610(99)70032-5			13	Microbiology	Microbiology	254VW	WOS:000083633700008	10575703				2021-04-07	
J	Delaroque, N; Maier, I; Knippers, R; Muller, DG				Delaroque, N; Maier, I; Knippers, R; Muller, DG			Persistent virus integration into the genome of its algal host, Ectocarpus siliculosus (Phaeophyceae)	JOURNAL OF GENERAL VIROLOGY			English	Article							MARINE BROWN-ALGAE; DNA-REPLICATION; FASCICULATUS	The brown alga Ectocarpus siliculosus frequently carries an endogenous virus, E, siliculosus virus (EsV-1), the genome of which is a circular, doublestranded DNA molecule of about 320 kbp, After infection, which occurs in the unicellular spores or gametes, the virus is present latently in all somatic cells of the host. Virus multiplication is restricted to cells of the reproductive organs. It has been an open question whether the latent viral DNA occurs as a free episome or becomes integrated into the host genome. PCR studies showed that viral DNA co-migrates with high molecular mass DNA in pulsed-field gel electrophoresis, which confirms that latent viral DNA is integrated into the host genome.	Univ Konstanz, Fak Biol, D-78457 Constance, Germany	Delaroque, N (corresponding author), Univ Konstanz, Fak Biol, D-78457 Constance, Germany.						Boehmer PE, 1997, ANNU REV BIOCHEM, V66, P347, DOI 10.1146/annurev.biochem.66.1.347; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Del Campo E, 1997, PHYCOLOGIA, V36, P186, DOI 10.2216/i0031-8884-36-3-186.1; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; Lee AM, 1998, VIROLOGY, V248, P35, DOI 10.1006/viro.1998.9245; LIU YG, 1994, NUCLEIC ACIDS RES, V22, P2168, DOI 10.1093/nar/22.11.2168; MOSIG G, 1995, FEMS MICROBIOL REV, V17, P83, DOI 10.1111/j.1574-6976.1995.tb00190.x; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1998, ADV VIRUS RES, V50, P49, DOI 10.1016/S0065-3527(08)60805-2; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; Zimmer R, 1997, GENOMICS, V42, P217, DOI 10.1006/geno.1997.4738	16	40	42	0	5	SOC GENERAL MICROBIOLOGY	READING	MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING, BERKS, ENGLAND RG7 1AE	0022-1317			J GEN VIROL	J. Gen. Virol.	JUN	1999	80		6				1367	1370		10.1099/0022-1317-80-6-1367			4	Biotechnology & Applied Microbiology; Virology	Biotechnology & Applied Microbiology; Virology	200LW	WOS:000080542600004	10374952	Bronze			2021-04-07	
J	Amsler, CD; Shelton, KL; Britton, CJ; Spencer, NY; Greer, GP				Amsler, CD; Shelton, KL; Britton, CJ; Spencer, NY; Greer, GP			Nutrients do not influence swimming behavior or settlement rates of Ectocarpus siliculosus (Phaeophyceae) spores	JOURNAL OF PHYCOLOGY			English	Article						behavior; chemotaxis; dispersal; Ectocarpus; nutrients; settlement; spores	ESCHERICHIA-COLI; PTERYGOPHORA-CALIFORNICA; MACROCYSTIS-PYRIFERA; ENTEROMORPHA; CHEMOTAXIS; ADHESION; KELPS; STIMULATION; PROPAGULES; DISPERSAL	Spores newly released from plurilocular sporangia of Ectocarpus siliculosus (Dillw,) Lyngb. sporophytes were assayed for chemotaxis to nutrients and for settlement stimulation by nutrients. To enable these measurements with relatively small volumes and numbers of released spores, we developed a computer-assisted motion-analysis assay for spore chemotaxis and verified the results with a more standard, capillary tube chemotaxis assay. The presence of a nutrient gradient did not influence the swimming behavior of E, siliculosus spores in the motion-analysis assay, and likewise no chemotactic effect was measured in the capillary tube assay, Microplate settlement assays previously utilized with bacteria and invertebrates were adapted for use with algal spores, E, siliculosus spores settled at higher rates on a hydrophobic plastic surface than on surfaces with either positively or negatively charged hydrophilic coating. Nutrient mixtures had no effect on the rate of spore settlement on hydrophobic surfaces.	Univ Alabama, Dept Biol, Birmingham, AL 35294 USA	Amsler, CD (corresponding author), Univ Alabama, Dept Biol, Birmingham, AL 35294 USA.		Spencer, Netanya/AAS-7999-2020	Spencer, Netanya/0000-0003-1010-5458; Amsler, Charles/0000-0002-4843-3759			AMSLER CD, 1993, J BACTERIOL, V175, P6238, DOI 10.1128/JB.175.19.6238-6244.1993; Amsler CD, 1996, ANAL BIOCHEM, V235, P20, DOI 10.1006/abio.1996.0086; AMSLER CD, 1990, MAR BIOL, V107, P297, DOI 10.1007/BF01319829; AMSLER CD, 1992, BRIT PHYCOL J, V27, P253, DOI 10.1080/00071619200650251; AMSLER CD, 1989, MAR BIOL, V102, P557, DOI 10.1007/BF00438358; Amsler CD, 1995, 2 COMPONENT SIGNAL T, P89; BERG HC, 1972, NATURE, V239, P500, DOI 10.1038/239500a0; Callow ME, 1997, J PHYCOL, V33, P938, DOI 10.1111/j.0022-3646.1997.00938.x; CHRISTIE AO, 1962, NATURE, V193, P193, DOI 10.1038/193193a0; CHRISTIE AO, 1973, P 3 INT C MAR CORR F, P674; DILLON PS, 1989, MICROBIAL ECOL, V17, P39, DOI 10.1007/BF02025592; FITT WK, 1990, MAR BIOL, V106, P389, DOI 10.1007/BF01344317; FLETCHER RL, 1984, MAR BIOL LETT, V5, P251; GELLER A, 1981, J EXP BIOL, V92, P53; Goff, 1989, ALGAE EXPT SYSTEMS, P201; GOLDEN L, 1984, Japanese Journal of Phycology, V32, P319; HARRISON PJ, 1980, J PHYCOL, V16, P28, DOI 10.1111/j.1529-8817.1980.tb00724.x; HENRY EC, 1982, J PHYCOL, V18, P550; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; Koshland D., 1980, DISTINGUISHED LECT S, V2; MAIER I, 1990, J EXP BOT, V41, P869, DOI 10.1093/jxb/41.7.869; MAKI JS, 1989, BIOL BULL, V177, P295, DOI 10.2307/1541944; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; MULLER DIETER G., 1964, Z BOT, V52, P193; Pawlik Joseph R., 1992, P189; PAWLIK JR, 1992, OCEANOGR MAR BIOL, V30, P273; Provasoli L., 1968, CULTURES COLLECTIONS, P63; REED DC, 1992, ECOLOGY, V73, P1577, DOI 10.2307/1940011; REED DC, 1988, ECOL MONOGR, V58, P321, DOI 10.2307/1942543; Rittschof D., 1988, P599; RITTSCHOF D, 1984, J EXP MAR BIOL ECOL, V82, P131, DOI 10.1016/0022-0981(84)90099-6; SAGER BM, 1988, ANAL BIOCHEM, V173, P271, DOI 10.1016/0003-2697(88)90189-3; SHEA C, 1990, BIOTECHNIQUES, V8, P610; SUTO S., 1950, BULL JAPANESE SOC SCI FISH, V16, P1; ZACKS DN, 1993, BIOPHYS J, V65, P508, DOI 10.1016/S0006-3495(93)81067-1; Zar JH., 1984, BIOSTATISTICAL ANAL; Zhu XY, 1996, J BACTERIOL, V178, P4208, DOI 10.1128/jb.178.14.4208-4215.1996	37	16	17	0	10	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044 USA	0022-3646			J PHYCOL	J. Phycol.	APR	1999	35	2					239	244		10.1046/j.1529-8817.1999.3520239.x			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	194YT	WOS:000080222900005					2021-04-07	
J	Xu, YN; Eichenberger, W				Xu, YN; Eichenberger, W			Phosphatidylglycerol of Ectocarpus fasciculatus (Phaeophyceae). Analysis of molecular species by the use of dinitrobenzoyl diacylglycerol derivatives	FETT-LIPID			English	Article							LIPID-METABOLISM; BETAINE LIPIDS; PHOSPHOLIPIDS; LEAVES; PLANTS		Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland	Xu, YN (corresponding author), Univ Bern, Dept Chem & Biochem, Freiestr 3, CH-3012 Bern, Switzerland.						BROWSE J, 1991, ANNU REV PLANT PHYS, V42, P467, DOI 10.1146/annurev.pp.42.060191.002343; CAHOON EB, 1994, PLANT PHYSIOL, V104, P827, DOI 10.1104/pp.104.3.827; DALMON J, 1981, PLANT SCI LETT, V21, P241, DOI 10.1016/0304-4211(81)90095-X; EICHENBERGER W, 1993, PHYTOCHEMISTRY, V34, P1323, DOI 10.1016/0031-9422(91)80024-U; FISCHER W, 1973, H-S Z PHYSIOL CHEM, V354, P1115, DOI 10.1515/bchm2.1973.354.2.1115; Frentzen M, 1998, FETT-LIPID, V100, P161; Hofmann M, 1998, PLANT CELL PHYSIOL, V39, P508, DOI 10.1093/oxfordjournals.pcp.a029398; Kunzler K, 1997, Z NATURFORSCH C, V52, P487; Makewicz A, 1997, PLANT CELL PHYSIOL, V38, P952, DOI 10.1093/oxfordjournals.pcp.a029257; Mazliak P., 1977, Lipids and lipid polymers in higher plants., P48; MULLER DG, 1972, PHYCOLOGIA, V11, P11; MURATA N, 1982, PLANT CELL PHYSIOL, V23, P1071; SEKIYA J, 1990, AGR BIOL CHEM TOKYO, V54, P2777, DOI 10.1080/00021369.1990.10870403; SHIBAHARA A, 1990, FEBS LETT, V264, P228, DOI 10.1016/0014-5793(90)80254-G; SOUTH GR, 1987, INTRO PHYCOL, P81; STARR RC, 1993, J PHYCOL S, V23, P1; TAKAMURA H, 1986, LIPIDS, V21, P356, DOI 10.1007/BF02535701; XU Y, 1998, ADV PLANT LIPID RES, P232; XU YN, 1996, THEORETICAL CONSIDER, V31, P223	19	6	8	0	0	WILEY-V C H VERLAG GMBH	BERLIN	MUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY	0931-5985			FETT-LIPID	Fett-Lipid	MAR	1999	101	3					104	108					5	Chemistry, Applied; Food Science & Technology	Chemistry; Food Science & Technology	182HB	WOS:000079492200004					2021-04-07	
J	Ehara, M; Watanabe, KI; Kawai, H; Inagaki, Y; Hayashi-Ishimaru, Y; Ohama, T				Ehara, M; Watanabe, KI; Kawai, H; Inagaki, Y; Hayashi-Ishimaru, Y; Ohama, T			Distribution of the mitochondrial deviant genetic code AUA for methionine in heterokont algae	JOURNAL OF PHYCOLOGY			English	Article						cytochrome oxidase subunit I; deviant genetic code; heterokont algae; Phaeophyceae; Xanthophyceae	RIBOSOMAL-RNA SEQUENCES; NUCLEOTIDE-SEQUENCES; TRIBONEMA-AEQUALE; XANTHOPHYCEAE; TRYPTOPHAN; EVOLUTION	The DNA sequence of the cytochrome oxidase subunit I (COXI) gene (1059 bp), was determined in a number of heterokont algae, including five species of the Phaeophyceae [Chorda filum (Linnaeus) Stackhouse, Colpomenia bullosa (Saunders) Yamada, Ectocarpus sp., Pseudochorda nagaii (Tokida) Inagaki, Undaria pinnatifida (Harvey) Suringar], and a member of the Raphidophyceae [Chattonella antiqua (Hada) Ono]. The distribution of a deviant mitochondrial code, the AUA codon for methionine (AUA/Met), which was previously reported in the Xanthophyceae, was inferred from these COXI sequences. Comparative analyses of these sequences revealed that all the algae described above bear the universal genetic code, including the assignment for the AUA codon. A phylogenetic tree was constructed using the obtained sequences along with already-published COXI sequences of various heterokont algae. The clusters of the Xanthophyceae and the Phaeophyceae were resolved as sister groups with high bootstrap support, excluding a bacillariophycean species, a raphidophycean species, and three species of the Eustigratophyceae. Taking the distribution of the deviant code and the COXI phylogenetic tree together, the genetic code change most probably occurred in an ancestor of the Xanthophyceae after it had branched off from the Phaeophyceae.	Osaka Univ, Fac Sci, Dept Biol, Toyonaka, Osaka 5600043, Japan; Kobe Univ, Res Ctr Inland Seas, Kobe, Hyogo 6578501, Japan	Ohama, T (corresponding author), JT Biohist Res Hall,1-1 Murasaki Cho, Takatsuki, Osaka 5691125, Japan.			Inagaki, Yuji/0000-0003-0101-8483			ADACHI J, 1992, COMPUTER SCI MONOGRA, V28; Anderson CL, 1997, J ECON BEHAV ORGAN, V33, P1, DOI 10.1016/S0167-2681(97)00018-8; ARIZTIA EV, 1991, J PHYCOL, V27, P428, DOI 10.1111/j.0022-3646.1991.00428.x; BOYEN C, 1994, NUCLEIC ACIDS RES, V22, P1400, DOI 10.1093/nar/22.8.1400; Ehara M, 1997, J MOL EVOL, V45, P119, DOI 10.1007/PL00006210; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; HayashiIshimaru Y, 1996, CURR GENET, V30, P29, DOI 10.1007/s002940050096; HayashiIshimaru Y, 1997, CURR GENET, V32, P296, DOI 10.1007/s002940050280; INAGAKI Y, 1998, IN PRESS J MOL EVOL; KAWAI H, 1995, J PHYCOL, V31, P306, DOI 10.1111/j.0022-3646.1995.00306.x; KIMURA M, 1980, J MOL EVOL, V16, P111, DOI 10.1007/BF01731581; LIM BL, 1986, JPN J GENET, V61, P169, DOI 10.1266/jjg.61.169; Nakamura Y, 1997, NUCLEIC ACIDS RES, V25, P244, DOI 10.1093/nar/25.1.244; OSAWA S, 1992, MICROBIOL REV, V56, P229, DOI 10.1128/MMBR.56.1.229-264.1992; Potter D, 1997, AM J BOT, V84, P966, DOI 10.2307/2446287; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; SAUNDERS GW, 1995, P NATL ACAD SCI USA, V92, P244, DOI 10.1073/pnas.92.1.244; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; SWOFFORD DL, 1993, PAUP PHYLOGENETIC AN; TOMPINS J, 1995, FDN CULTURE COLLECTI; VanderAuwera G, 1997, J MOL EVOL, V45, P84, DOI 10.1007/PL00006205; Watanabe MM, 1994, NIES COLLECTION LIST	22	2	3	0	3	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044 USA	0022-3646			J PHYCOL	J. Phycol.	DEC	1998	34	6					1005	1008		10.1046/j.1529-8817.1998.341005.x			4	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	155VB	WOS:000077966100013					2021-04-07	
J	Giraldez, N; Aparicio, PJ; Quinones, MA				Giraldez, N; Aparicio, PJ; Quinones, MA			Blue light requirement for HCO3- uptake and its action spectrum in Monoraphidium braunii	PHOTOCHEMISTRY AND PHOTOBIOLOGY			English	Article							ALGA CHLAMYDOMONAS-REINHARDTII; INORGANIC CARBON ACQUISITION; SATURATED PHOTOSYNTHESIS; ANHYDRASE ACTIVITY; CO2; STIMULATION; ECTOCARPUS; TRANSPORT; CYANOBACTERIA; TRANSDUCTION	The uptake and assimilation of HCO3- by the green unicellular alga Monoraphidium braunii can be monitored by the alkalinization of the external medium or by the O-2 evolution associated with the uptake and reduction of this anion, The activation of HCO3- uptake in this microalga required the irradiation of the cell suspensions with low photon fluence rates of short wavelength radiation. Thus, when the cells were irradiated with strong red light in the presence of HCO3-, very little alkalinization of the external medium or O-2 evolution could be observed. The O-2 evolution rates measured under red light could be due to the assimilation of the CO2 derived from the HCO3- present in the medium, The blue light-dependent Oz evolution rates were not diminished by a periplasmic carbonic anhydrase inhibitor, suggesting that HCO(3)(-)dependent O-2 evolution was due to the photoactivation of a selective HCO3- uptake system at the plasma membrane. The action spectrum for HCO3- uptake in M. braunii was very similar to those reported for NO3- and Cl- and suggested that a flavoprotein may be the photoreceptor for this response.	Univ Lund, Dept Plant Physiol, S-22100 Lund, Sweden; CSIC, Ctr Invest Biol, Madrid, Spain	Quinones, MA (corresponding author), Univ Lund, Dept Plant Physiol, Box 117, S-22100 Lund, Sweden.		Quinones, Miguel/AAB-3714-2019	Quinones, Miguel/0000-0002-8814-0440			AIZAWA K, 1986, FEMS MICROBIOL LETT, V39, P215, DOI 10.1016/0378-1097(86)90447-7; APARICIO PJ, 1994, PLANT CELL ENVIRON, V17, P1323, DOI 10.1111/j.1365-3040.1994.tb00534.x; Assmann SM, 1996, CURR OPIN CELL BIOL, V8, P458, DOI 10.1016/S0955-0674(96)80021-4; BADGER MR, 1992, PHYSIOL PLANTARUM, V84, P606, DOI 10.1111/j.1399-3054.1992.tb04711.x; BADGER MR, 1994, ANNU REV PLANT PHYS, V45, P369, DOI 10.1146/annurev.pp.45.060194.002101; Chamovitz DA, 1996, CRIT REV PLANT SCI, V15, P455, DOI 10.1080/07352689609382367; DIONISIO ML, 1989, PLANT CELL PHYSIOL, V30, P215, DOI 10.1093/oxfordjournals.pcp.a077732; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; Eriksson M, 1998, PLANT PHYSIOL, V116, P637, DOI 10.1104/pp.116.2.637; FINDENEGG GR, 1979, PLANT SCI LETT, V17, P101, DOI 10.1016/0304-4211(79)90168-8; FOSTER RM, 1992, PLANT CELL ENVIRON, V15, P241; GUERRERO MG, 1981, ANNU REV PLANT PHYS, V32, P169, DOI 10.1146/annurev.pp.32.060181.001125; JAGENDORF AT, 1975, BIOENERG PHOTOSYNTH, P414; Jenkins GI, 1995, PLANT SCI, V112, P117, DOI 10.1016/0168-9452(95)04260-1; KAUFMAN LS, 1993, PLANT PHYSIOL, V102, P333, DOI 10.1104/pp.102.2.333; MAATHUIS F J M, 1992, Current Opinion in Cell Biology, V4, P661, DOI 10.1016/0955-0674(92)90087-S; Maier J, 1997, J PHOTOCH PHOTOBIO B, V38, P274, DOI 10.1016/S1011-1344(96)07464-7; Miyachi S., 1985, INORGANIC CARBON UPT, P145; PALMQVIST K, 1994, PHYSIOL PLANTARUM, V90, P537; PALMQVIST K, 1990, PHYSIOL PLANTARUM, V80, P267, DOI 10.1034/j.1399-3054.1990.800217.x; POOLE RJ, 1978, ANNU REV PLANT PHYS, V29, P437, DOI 10.1146/annurev.pp.29.060178.002253; QUINONES MA, 1990, PHOTOCHEM PHOTOBIOL, V51, P689, DOI 10.1111/php.1990.51.6.689; Quinones Maria I., 1990, THESIS; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; SHORT TW, 1994, ANNU REV PLANT PHYS, V45, P143, DOI 10.1146/annurev.pp.45.060194.001043; SPALDING MH, 1989, AQUAT BOT, V34, P181, DOI 10.1016/0304-3770(89)90056-9; SULTEMEYER DF, 1989, PLANT PHYSIOL, V89, P1213, DOI 10.1104/pp.89.4.1213; THIELMANN J, 1990, PLANT PHYSIOL, V92, P622, DOI 10.1104/pp.92.3.622; WILBUR KM, 1948, J BIOL CHEM, V176, P147; WILLIAM JL, 1983, J MEMBRANE BIOL, V73, P263; WILLIAM JL, 1983, ANNU REV PLANT PHYS, V34, P71; WILLIAMS TG, 1987, PLANT PHYSIOL, V83, P92, DOI 10.1104/pp.83.1.92; WITT FG, 1995, PHOTOCHEM PHOTOBIOL, V61, P619, DOI 10.1111/j.1751-1097.1995.tb09878.x	35	10	10	0	5	AMER SOC PHOTOBIOLOGY	AUGUSTA	BIOTECH PARK, 1021 15TH ST, SUITE 9, AUGUSTA, GA 30901-3158 USA	0031-8655			PHOTOCHEM PHOTOBIOL	Photochem. Photobiol.	SEP	1998	68	3					420	426					7	Biochemistry & Molecular Biology; Biophysics	Biochemistry & Molecular Biology; Biophysics	120YY	WOS:000075986500031					2021-04-07	
J	Abd-El-Monem, HM; Corradi, MG; Gorbi, G				Abd-El-Monem, HM; Corradi, MG; Gorbi, G			Toxicity of copper and zinc to two strains of Scenedesmus acutus having different sensitivity to chromium	ENVIRONMENTAL AND EXPERIMENTAL BOTANY			English	Article						chromium; copper; co-tolerance; Scenedesmus acutus; zinc	LINKED TROPHIC LEVELS; MARINE FOULING ALGA; CHLAMYDOMONAS-REINHARDTII; ECTOCARPUS-SILICULOSUS; DAPHNIA-MAGNA; HEAVY-METALS; GREEN-ALGA; TOLERANCE; CADMIUM; GROWTH	A Cr-tolerant strain of Scenedesmus acutus (Chlorophyceae) was cultured in the presence of different Cu2+ or Zn2+ concentrations to evaluate if it was also tolerant to these metals and consequently maintained unaltered its nutritional value for consumer organisms. The evaluation of tolerance was based on culture growth and recovery rates which were compared with those of the wild type. The evaluation of the nutritional value was based on quantitative determinations of dry mass and carbohydrate and protein content. In the presence of Cu concentrations that were toxic to the wild type, the Cr-tolerant strain grew better and, when transferred to metal-fret culture medium, resumed growth more quickly. It also maintained fluorescence and unchanged protein levels. This suggests that the Cr-tolerant strain is also less sensitive to copper. However, no evidence of a lower sensitivity to zinc was found. (C) 1998 Elsevier Science B.V. All rights reserved.	Univ Parma, Dept Evolut & Funct Biol, I-43100 Parma, Italy; Menoufia Univ, Dept Bot, Menoufia, Egypt; Univ Parma, Dept Environm Sci, I-43100 Parma, Italy	Corradi, MG (corresponding author), Univ Parma, Dept Evolut & Funct Biol, I-43100 Parma, Italy.		Gorbi, Gessica/B-5581-2015	Gorbi, Gessica/0000-0002-0933-6032			BARIAUD A, 1984, B ENVIRON CONTAM TOX, V32, P597, DOI 10.1007/BF01607543; BARUA B, 1986, PHOTOSYNTHETICA, V20, P74; BERTRAM PE, 1979, ENVIRON POLLUT, V19, P295, DOI 10.1016/0013-9327(79)90121-6; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; BUTLER M, 1980, PLANT CELL ENVIRON, V3, P119; Chiaudani G., 1977, NUOVI ANN IG MICROB, V28, P145; CORRADI MG, 1993, ECOTOX ENVIRON SAFE, V25, P72, DOI 10.1006/eesa.1993.1008; CORRADI MG, 1995, ECOTOX ENVIRON SAFE, V32, P12, DOI 10.1006/eesa.1995.1079; DEFILIPPIS LF, 1979, Z PFLANZENPHYSIOL, V92, P39, DOI 10.1016/S0044-328X(79)80151-8; FERNANDES JC, 1991, BOT REV, V57, P246, DOI 10.1007/BF02858564; GEKELER W, 1988, ARCH MICROBIOL, V150, P197, DOI 10.1007/BF00425162; GORBI G, 1993, ECOTOX ENVIRON SAFE, V25, P64, DOI 10.1006/eesa.1993.1007; Gorbi G, 1996, ECOTOX ENVIRON SAFE, V35, P109, DOI 10.1006/eesa.1996.0089; GUPTA SL, 1986, PHOTOSYNTHETICA, V20, P447; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; HALL A, 1980, NEW PHYTOL, V85, P73, DOI 10.1111/j.1469-8137.1980.tb04449.x; Herbert D, 1971, METHODS MICROBIOLO B, P209, DOI DOI 10.1016/S0580-9517(08)70641-X; HOWE G, 1992, PLANT PHYSIOL, V98, P127, DOI 10.1104/pp.98.1.127; LUMSDEN BR, 1983, ENVIRON TECHNOL LETT, V4, P271, DOI 10.1080/09593338309384205; MACFIE SM, 1994, ARCH ENVIRON CON TOX, V27, P454; Moore J. W., 1983, HEAVY METALS NATURAL; Schat H, 1996, EVOLUTION, V50, P1888, DOI 10.1111/j.1558-5646.1996.tb03576.x; STEFFENS JC, 1990, ANNU REV PLANT PHYS, V41, P553, DOI 10.1146/annurev.pp.41.060190.003005; STIBOROVA M, 1986, PHOTOSYNTHETICA, V20, P418; STOKES PM, 1981, CAN J BOT, V59, P1817, DOI 10.1139/b81-242; TWISS MR, 1993, CAN J BOT, V71, P333, DOI 10.1139/b93-035; VISVIKI I, 1991, ARCH ENVIRON CON TOX, V20, P271, DOI 10.1007/BF01055915	28	30	32	0	13	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND	0098-8472			ENVIRON EXP BOT	Environ. Exp. Bot.	AUG	1998	40	1					59	66					8	Plant Sciences; Environmental Sciences	Plant Sciences; Environmental Sciences & Ecology	ZZ799	WOS:000074768300006					2021-04-07	
J	Maier, I; Wolf, S; Delaroque, N; Muller, DG; Kawai, H				Maier, I; Wolf, S; Delaroque, N; Muller, DG; Kawai, H			A DNA virus infecting the marine brown alga Pilayella littoralis (Ectocarpales, Phaeophyceae) in culture	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						algae; Ectocarpales; marine double-stranded DNA virus; Phaeophyceae; phycovirus; Pilayella littoralis; PlitV-1	SILICULOSUS PHAEOPHYCEAE; EUKARYOTIC ALGAE; FASCICULATUS; PARTICLES; GENOME	A new large DNA virus (PlitV-1) infects the marine filamentous brown alga Pilayella littoralis. It was collected in Alaska and infects other P. littoralis isolates of different geographic origin. The virus has an icosahedral capsid of c. 161 nm in diameter, enclosing an electron-dense core. The genome consists of double-stranded DNA and is approximately 280 000 base pairs in size. The virus is latent in somatic cells of the host and is propagated only upon induction of the host's reproductive organs. It causes deformed sporangia, resulting in infertility, and is structurally similar to other brown algal viruses. PCR amplification of a genomic sequence coding for part of a structural glycoprotein of the Ectocarpus siliculosus virus EsV-1 produced a fragment of similar size to that obtained with EsV-1.	Univ Konstanz, Fak Biol, D-78457 Constance, Germany; Kobe Univ, Res Ctr Inland Seas, Kobe, Hyogo 657, Japan	Maier, I (corresponding author), Univ Konstanz, Fak Biol, D-78457 Constance, Germany.						BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; CARDINAL A, 1964, BEIH NOV HEDW, V15; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; JEPPESEN C, 1989, EUR J BIOCHEM, V182, P437, DOI 10.1111/j.1432-1033.1989.tb14850.x; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KORNMANN P, 1978, MEERESALGEN HELGOLAN; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; Maier I, 1997, J PHYCOL, V33, P838, DOI 10.1111/j.0022-3646.1997.00838.x; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; Maier I, 1998, PHYCOLOGIA, V37, P60, DOI 10.2216/i0031-8884-37-1-60.1; MARKEY D R, 1974, Protoplasma, V80, P223, DOI 10.1007/BF01666361; MULLER DG, 1989, BOT MAR, V32, P71, DOI 10.1515/botm.1989.32.1.71; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; MULLER DG, 1998, IN PRESS ADV VIRUS R, V50; MULLER DG, 1996, HYDROBIOLOGIA, V21, P21; REISSER W, 1993, ARCH PROTISTENKD, V143, P257, DOI 10.1016/S0003-9365(11)80293-9; REISSER W, 1995, ALGAE ENV HUMAN AFFA, P143; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VENABLE JH, 1965, J CELL BIOL, V25, P407, DOI 10.1083/jcb.25.2.407; WILLIAMS MA, 1977, PRACTICAL METHODS EL, V6, P63; WOLF S, 1998, IN PRESS PROTOPLASMA	27	16	16	0	11	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	AUG	1998	33	3					213	220		10.1017/S0967026298001747			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	128DY	WOS:000076391000003					2021-04-07	
J	Lee, AM; Ivey, RG; Meints, RH				Lee, AM; Ivey, RG; Meints, RH			The DNA polymerase gene of a brown algal virus: Structure and phylogeny	JOURNAL OF PHYCOLOGY			English	Article						brown algae; DNA polymerase; Feldmannia sp.; FsV-1; Phaeophyceae; phylogeny; virus	NUCLEAR POLYHEDROSIS-VIRUS; NUCLEOTIDE-SEQUENCE; ECTOCARPUS-SILICULOSUS; TRANSCRIPTION ANALYSIS; PROTEIN SEQUENCES; GENOME; ALIGNMENT; CLONING; PBCV-1; IDENTIFICATION	We have reported the complete sequence of the DNA polymerase gene from the virus that infected a filamentous brown alga, Feldmannia sp. (FsV). The DNA polymerase gene from FsV encoded 986 amino acids and contained all the consented motifs of 3'-5' exonuclease domains and catalytic domains found in B-family (alpha-like) DNA polymerases. The codons for the FsV DNA polymerase appeared to have some bias toward guanine/cytosine (G/C) in the third position. The phylogenetic analysis of the FsV DNA polymerase gene and other viral DNA polymerase genes indicated that FsV belongs to a family of algal viruses recently defined as Phycodnaviridae.	Oregon State Univ, Ctr Gene Res & Biotechnol, Corvallis, OR 97331 USA; Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA	Meints, RH (corresponding author), Oregon State Univ, Ctr Gene Res & Biotechnol, Corvallis, OR 97331 USA.	meinstr@bcc.orst.edu					ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1016/S0022-2836(05)80360-2; BAER R, 1984, NATURE, V310, P207, DOI 10.1038/310207a0; BERTHOMME H, 1995, J VIROL, V69, P2811, DOI 10.1128/JVI.69.5.2811-2818.1995; BINNS MM, 1987, NUCLEIC ACIDS RES, V15, P6563, DOI 10.1093/nar/15.16.6563; BJORNSON RM, 1992, J GEN VIROL, V73, P3177, DOI 10.1099/0022-1317-73-12-3177; BRAITHWAITE DK, 1993, NUCLEIC ACIDS RES, V21, P787, DOI 10.1093/nar/21.4.787; Chen F, 1996, VIROLOGY, V219, P170, DOI 10.1006/viro.1996.0234; CHEN F, 1995, APPL ENVIRON MICROB, V61, P1274, DOI 10.1128/AEM.61.4.1274-1278.1995; COWAN P, 1994, J GEN VIROL, V75, P3211, DOI 10.1099/0022-1317-75-11-3211; DAVISON AJ, 1986, J GEN VIROL, V67, P1759, DOI 10.1099/0022-1317-67-9-1759; DEVEREUX J, 1984, NUCLEIC ACIDS RES, V12, P387, DOI 10.1093/nar/12.1Part1.387; EARL PL, 1986, P NATL ACAD SCI USA, V83, P3659, DOI 10.1073/pnas.83.11.3659; ELLIOTT R, 1991, VIROLOGY, V185, P169, DOI 10.1016/0042-6822(91)90765-4; FELSENSTEIN J, 1988, ANNU REV GENET, V22, P521, DOI 10.1146/annurev.ge.22.120188.002513; Felsenstein J, 1996, METHOD ENZYMOL, V266, P418; Felsenstein J., 1993, PHYLIP PHYLOGENY INF; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; GRABHERR R, 1992, VIROLOGY, V188, P721, DOI 10.1016/0042-6822(92)90527-V; GRAVES MV, 1992, VIROLOGY, V188, P198, DOI 10.1016/0042-6822(92)90750-J; GRAVES MV, 1992, GENE, V113, P149, DOI 10.1016/0378-1119(92)90390-B; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; Higgins D G, 1994, Methods Mol Biol, V25, P307; HILLIS DM, 1993, SYST BIOL, V42, P182, DOI 10.2307/2992540; ITO J, 1991, NUCLEIC ACIDS RES, V19, P4045, DOI 10.1093/nar/19.15.4045; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; KOUZARIDES T, 1987, J VIROL, V61, P125, DOI 10.1128/JVI.61.1.125-133.1987; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LARDER BA, 1987, EMBO J, V6, P169, DOI 10.1002/j.1460-2075.1987.tb04735.x; LEE AM, 1995, VIROLOGY, V212, P474, DOI 10.1006/viro.1995.1505; Mao JH, 1997, VIROLOGY, V229, P212, DOI 10.1006/viro.1996.8435; MARTINS A, 1994, NUCLEIC ACIDS RES, V22, P208, DOI 10.1093/nar/22.2.208; MEINTS RH, 1984, VIROLOGY, V138, P341, DOI 10.1016/0042-6822(84)90358-1; Muller DG, 1996, PHYCOLOGIA, V35, P61, DOI 10.2216/i0031-8884-35-1-61.1; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; Mustafa A, 1991, DNA Seq, V2, P39, DOI 10.3109/10425179109008437; ROHOZINSKI J, 1989, VIROLOGY, V168, P363, DOI 10.1016/0042-6822(89)90277-8; SCHLEISS MR, 1994, VIROLOGY, V202, P173, DOI 10.1006/viro.1994.1333; Tidona CA, 1997, VIROLOGY, V230, P207, DOI 10.1006/viro.1997.8456; TOMALSKI MD, 1988, VIROLOGY, V167, P591, DOI 10.1016/S0042-6822(88)90122-5; TSURUMI T, 1987, GENE, V52, P129; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; YUEN L, 1987, P NATL ACAD SCI USA, V84, P6417, DOI 10.1073/pnas.84.18.6417	45	15	15	0	1	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044 USA	0022-3646			J PHYCOL	J. Phycol.	AUG	1998	34	4					608	615		10.1046/j.1529-8817.1998.340608.x			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	116FB	WOS:000075712400006					2021-04-07	
J	Burkhardt, E; Peters, AF				Burkhardt, E; Peters, AF			Molecular evidence from nrDNA its sequences that Laminariocolax (Phaeophyceae, Ectocarpales sensu lato) is a worldwide clade of closely related kelp endophytes	JOURNAL OF PHYCOLOGY			English	Article						biogeography; endophyte; internal transcribed spacer; Laminariales; Laminariocolax; nuclear ribosomal DNA; pathogen; small subunit; taxonomy	GREEN PATCH DISEASE; RIBOSOMAL-RNA GENES; NUCLEOTIDE-SEQUENCES; LIFE-HISTORY; LAMINARIALES PHAEOPHYTA; RED ALGAE; SP-NOV; CHLOROPHYTA; RHODOPHYTA; CULTURE	Marine brown algae living as endophytes in macroalgae are morphologically simple and their taxonomy is particularly difficult. A molecular phylogeny for endophytic taxa isolated from kelps and red algae, and for putative epiphytic and free-living relatives, was inferred from partial small subunit and complete internal transcribed spacer nuclear ribosomal DNA sequences. It has revealed the following results. (1) Three species of endophytes isolated from members of the Laminariales are closely related. They form a clade together with the epi-endophytic species Laminariocolax tomentosoides (Farlow) Kylin. Members of the clade possess uniseriate plurilocular sporangia, and they may form erect filaments. Laminariocolax eckloniae sp. nov., occurring in the South African host Ecklonia maxima (Osbeck) Papenfuss, is described. The new combinations, Laminariocolax aecidioides (Rosenvinge) comb. nov, and L. macrocystis (Peters) comb, nov., are proposed for two taxa previously classified in Gononema and Streblonema, respectively. (2) The genus Laminariocolax occurs worldwide in temperate areas, and the phylogeny of the taxa studied is in agreement with biogeographic distribution. (3) Laminariocolax belongs to the Ectocarpales sensu lato. The genus is more closely related to Chordaria than to Dictyosiphon, Ectocarpus, or Scytosiphon. (4) Two brown endophytes (Streblonema spp.), isolated from red algae, are closely related to each other and may form a sister clade to Laminariocolax.	Inst Meereskunde, Abt Meeresbot & AG Marine Pathol, D-24105 Kiel, Germany; Univ Kiel, Inst Bot, D-24098 Kiel, Germany	Burkhardt, E (corresponding author), Inst Meereskunde, Abt Meeresbot & AG Marine Pathol, Dusternbrooker Weg 20, D-24105 Kiel, Germany.						APT KE, 1988, J PHYCOL, V24, P28; ARIZTIA EV, 1991, J PHYCOL, V27, P428, DOI 10.1111/j.0022-3646.1991.00428.x; BAKKER FT, 1995, EUR J PHYCOL, V30, P197, DOI 10.1080/09670269500650981; BHATTACHARYA D, 1992, EVOLUTION, V46, P1801, DOI 10.1111/j.1558-5646.1992.tb01170.x; Correa JA, 1997, J PHYCOL, V33, P344, DOI 10.1111/j.0022-3646.1997.00344.x; CORREA JA, 1994, DIS AQUAT ORGAN, V19, P203, DOI 10.3354/dao019203; CORREA JA, 1988, J PHYCOL, V24, P528, DOI 10.1111/j.1529-8817.1988.tb04258.x; DANGEARD P, 1970, Botaniste, V53, P23; de Saunders A, 1901, P WASH ACAD SCI, V3, P391; Derbes A., 1851, CATALOGUE PLANTES S, P93; Ellertsdottir E, 1997, MAR ECOL PROG SER, V146, P135, DOI 10.3354/meps146135; Fritsch FE, 1945, STRUCTURE REPROD ALG; Goff LJ, 1996, J PHYCOL, V32, P297, DOI 10.1111/j.0022-3646.1996.00297.x; GOFF LJ, 1994, J PHYCOL, V30, P695, DOI 10.1111/j.0022-3646.1994.00695.x; GREVILLE RK, 1827, SCOTTISH CRYPTOGAMIC, P271; HEESCH S, 1996, THESIS U KIEL; KAWAI H, 1995, J PHYCOL, V31, P306, DOI 10.1111/j.0022-3646.1995.00306.x; Kawai Hiroshi, 1995, Phycological Research, V43, P185, DOI 10.1111/j.1440-1835.1995.tb00024.x; KIMURA M, 1980, J MOL EVOL, V16, P111, DOI 10.1007/BF01731581; KUCKUCK P, 1954, STREBLONEMA HELGOLAN, V5, P103; KUCKUCK P, 1894, WISS MEERESUNTERSUCH, V1, P223; Kumar S, 1993, MEGA MOL EVOLUTIONAR; KYLIN H, 1947, LUNDS U ARSSKR, V43, P1; Kylin H., 1937, ACTA U LUND, V33, P1; LEIN TE, 1991, SARSIA, V76, P187, DOI 10.1080/00364827.1991.10413474; LOISEAUX S, 1970, Phycologia, V9, P185, DOI 10.2216/i0031-8884-9-2-185.1; LOISEAUX S, 1969, PHYCOLOGIA, V8, P1; Nielsen Ruth, 1995, Acta Botanica Fennica, V155, P1; PEDERSEN P.M., 1984, OPERA BOT, V74, P1; PEDERSEN PM, 1981, NORD J BOT, V1, P263, DOI DOI 10.1111/j.1756-1051.1981.tb00695.x; Peters A.F., 1987, PROGR PHYCOLOGICAL R, V5, P223; PETERS AF, 1988, BRIT PHYCOL J, V23, P299, DOI 10.1080/00071618800650331; PETERS AF, 1992, BRIT PHYCOL J, V27, P177, DOI 10.1080/00071619200650181; PETERS AF, 1985, HELGOLANDER MEERESUN, V39, P441, DOI 10.1007/BF01987412; PETERS AF, 1990, CAN J BOT, V68, P1398, DOI 10.1139/b90-178; PETERS AF, 1991, PHYCOLOGIA, V30, P365, DOI 10.2216/i0031-8884-30-4-365.1; Peters AF, 1996, NOVA HEDWIGIA, V62, P341; Peters AF, 1997, J PHYCOL, V33, P294, DOI 10.1111/j.0022-3646.1997.00294.x; Peters AF, 1998, PHYCOLOGIA, V37, P114, DOI 10.2216/i0031-8884-37-2-114.1; Peters Akira F., 1996, Hydrobiologia, V326-327, P111, DOI 10.1007/BF00047795; PRINGSHEIM N, 1863, ABH KONIGL AKAD WISS, P1; REINSCH PF, 1875, CONTRIBUTIONES ALGOL; Rosenvinge L. K. R., 1893, MEDD GROENL, V3, P765; RUSSELL G, 1964, J MAR BIOL ASSOC UK, V44, P601, DOI 10.1017/S0025315400027806; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; Sanchez P, 1996, EUR J PHYCOL, V31, P173, DOI 10.1080/09670269600651351; Saunders Gary W., 1993, Hydrobiologia, V260-261, P689, DOI 10.1007/BF00049089; SAUNDERS GW, 1993, MAR BIOL, V115, P347, DOI 10.1007/BF00349831; SAUNDERS GW, 1992, J PHYCOL, V28, P544, DOI 10.1111/j.0022-3646.1992.00544.x; SAUNDERS GW, 1995, PHYCOLOGIA, V34, P383, DOI 10.2216/i0031-8884-34-5-383.1; Sauvageau C., 1898, ANN SC NAT BOT, V5, P161; Setchell W.A., 1922, U CALIF PUBL BOT, VV, P385; SKOTTSBERG C, 1921, K SVENSKA VETENSK AK, V61, P1; SOUTH G R, 1974, Journal of the Royal Society of New Zealand, V4, P455; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; Stromfelt H. F. G., 1888, NOTARISIA, V3, P381; SWOFFORD DL, 1993, PAUP PHYLOGENETIC AN; TAN IH, 1994, J PHYCOL, V30, P721, DOI 10.1111/j.0022-3646.1994.00721.x; Tan IH, 1996, J PHYCOL, V32, P112, DOI 10.1111/j.0022-3646.1996.00112.x; VANOPPEN MJH, 1993, MAR BIOL, V115, P381, DOI 10.1007/BF00349835; VEIGA AJ, 1997, AN JARDIN BOT MADRID, V55, P155; Yoshida T., 1979, P 9 INT SEAW S, P219; ZINOVA AD, 1953, DETERMINATION BROWN	63	28	31	0	10	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044 USA	0022-3646			J PHYCOL	J. Phycol.	AUG	1998	34	4					682	691		10.1046/j.1529-8817.1998.340682.x			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	116FB	WOS:000075712400016					2021-04-07	
J	van Wijk, DJ; Kroon, SGM; Garttener-Arends, ICM				van Wijk, DJ; Kroon, SGM; Garttener-Arends, ICM			Toxicity of chlorate and chlorite to selected species of algae, bacteria, and fungi	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY			English	Article						chlorate; chlorite; aquatic toxicity	REDUCTION	The present study confirms that chlorate is toxic only to brown algae and not to species of other ecologically relevant taxa, The brown alga Ectocarpus variabilis exhibited a LOEC of 0.005 mM (0.4 mg ClO3-/liter) and an LC50 of 0.012 mM, when cultured with nitrate as a sole source of nitrogen. The toxicity to species other than brown algae as measured in growth inhibition tests ranged from 0.75 mM (96-h NOEC) for Selenastrum capricornutum to greater than or equal to 7.48 mM (48-h NOEC) for the fungus Trichoderma hamatum. The nitrogen source, nitrate or ammonium, did not significantly influence the toxicity to the nonsensitive species. The tests on brown algae found that as compared with ammonium, the toxicity to nitrate-grown cultures is higher by a factor of about 10. This confirms the hypothesis that nitrate reductase is involved in the toxic effects of chlorate on brown algae. Chlorite, tested as a potential toxic metabolite of chlorate, demonstrated high toxicity to many of the taxa tested and only low toxicity to E. variabilis, It may be concluded that brown algae are exceptionally sensitive to chlorate. It may also be concluded that various nitrogen sources could not induce toxicity in nonsensitive species. From these experiments no conclusions could be drawn as to the potential role of chlorite in chlorate toxicity, Furthermore it may be concluded that E. variabilis is a suitable laboratory test species for further investigations into the mechanism of chlorate toxicity to brown algae, (C) 1998 Academic Press.	Akzo Corp Res, Cent Res, Dept Gen Analyt & Environm Chem, NL-6800 SB Arnhem, Netherlands	van Wijk, DJ (corresponding author), Akzo Corp Res, Cent Res, Dept Gen Analyt & Environm Chem, POB 9300, NL-6800 SB Arnhem, Netherlands.						CAPUZZO JM, 1979, ESTUAR COAST MAR SCI, V8, P307, DOI 10.1016/0302-3524(79)90048-3; *DIN, 1992, 38412 DIN; *DIN, 1991, 38412 DIN; *DSMZ, 1989, DTSCH SAMML MIKR ZEL; *EEC, 1989, METH DET EC C3 ALG I; *EEC, 1988, OFF J EUR COMMUNIT L, V133; *EPA, 1989, FRESHW MAR ALG AC TO; FAHRAEUS G, 1952, ACTA CHEM SCAND, V5, P1416; GOKSOYR J, 1951, PHYSIOL PLANTARUM, V4, P498, DOI 10.1111/j.1399-3054.1951.tb07687.x; *ISO, 1996, 10712 ISO; LILJESTROM STIG, 1966, LANT BRUKSHOGSKOLANS ANN, V32, P93; *NERC, 1988, CULT COLL ALG PROT; Organisation for Economic Co-operation and Development (OECD), 1984, GUID TEST CHEM ALG G; Rikken GB, 1996, APPL MICROBIOL BIOT, V45, P420, DOI 10.1007/s002530050707; ROSEMARIN A, 1994, ENVIRON POLLUT, V85, P3, DOI 10.1016/0269-7491(94)90233-X; ROSEMARIN A, 1986, OPHELIA S, V4, P219; SOLOMONS.LP, 1972, PLANT PHYSIOL, V50, P421, DOI 10.1104/pp.50.4.421; *US FDA, 1987, 402 FDA; VANGINKEL CG, 1995, CHEMOSPHERE, V31, P4057, DOI 10.1016/0045-6535(95)80007-8; vanGlinkel CG, 1996, ARCH MICROBIOL, V166, P321, DOI 10.1007/s002030050390; vanWijk DJ, 1995, ECOTOX ENVIRON SAFE, V32, P244, DOI 10.1006/eesa.1995.1110; WHEELER WN, 1982, P C BAMF MAR STAT BA, P121; WILLIAMS DA, 1972, BIOMETRICS, V28, P519, DOI 10.2307/2556164; ZERBE GO, 1987, AM STAT, V32, P3	24	43	43	0	17	ACADEMIC PRESS INC	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA	0147-6513			ECOTOX ENVIRON SAFE	Ecotox. Environ. Safe.	JUL	1998	40	3					206	211		10.1006/eesa.1998.1685			6	Environmental Sciences; Toxicology	Environmental Sciences & Ecology; Toxicology	102AL	WOS:000074901000005	9679683				2021-04-07	
J	Fuller, NJ; Wilson, WH; Joint, IR; Mann, NH				Fuller, NJ; Wilson, WH; Joint, IR; Mann, NH			Occurrence of a sequence in marine cyanophages similar to that of T4 g20 and its application to PCR-based detection and quantification techniques	APPLIED AND ENVIRONMENTAL MICROBIOLOGY			English	Article							DNA-POLYMERASE GENES; ECTOCARPUS-SILICULOSUS; MICROBIAL COMMUNITIES; MICROMONAS-PUSILLA; TAILED PHAGES; VIRUSES; SYNECHOCOCCUS; BACTERIA; BACTERIOPHAGE-T4; DIVERSITY	Viruses are ubiquitous components of marine ecosystems and are known to infect unicellular phycoerythrin-containing cyanobacteria belonging to the genus Synechococcus. A conserved region from the cyanophage genome was identified in three genetically distinct cyanomyoviruses, and a sequence analysis revealed that this region exhibited significant similarity to a gene encoding a capsid assembly protein (gp20) from the enteric coliphage T4. The results of a comparison of gene 20 sequences from three cyanomyoviruses and T4 allowed us to design two degenerate PCR primers, CPS1 and CPS2 which specifically amplified a 165-bp region from the majority of cyanomyoviruses tested. A competitive PCR (cPCR) analysis revealed that cyanomyovirus strains could be accurately enumerated, and it was demonstrated that quantification was log-linear over ca. 3 orders of magnitude. Different calibration curves were obtained for each of the three cyanomyovirus strains tested; consequently, cPCR performed with primers CPS1 and CPS2 could lead to substantial inaccuracies in estimates of phage abundance in natural assemblages, Further sequence analysis of cyanomyovirus gene 20 homologs would be necessary in order to design primers which do not exhibit phage-to-phage variability in priming efficiency. It was demonstrated that PCR products of the correct size could be amplified from seawater samples following 100x concentration and even directly without any prior concentration. Hence, the use of degenerate primers in PCR analyses of cyanophage populations should provide valuable data on the diversity of cyanophages in natural assemblages. Further optimization of procedures may ultimately lead to a sensitive assay which can be used to analyze natural cyanophage populations both quantitatively (by cPCR) and qualitatively following phylogenetic analysis of amplified products.	Marine Biol Assoc United Kingdom Lab, Plymouth PL1 2PB, Devon, England; Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England; Plymouth Marine Lab, Plymouth PL1 3DH, Devon, England	Wilson, WH (corresponding author), Marine Biol Assoc United Kingdom Lab, Citadel Hill, Plymouth PL1 2PB, Devon, England.	whw@wpo.nerc.ac.uk					ACKERMANN HW, 1984, INTERVIROLOGY, V22, P61, DOI 10.1159/000149535; ACKERMANN HW, 1995, ARCH VIROL, V140, P1871, DOI 10.1007/BF01384350; BOEHME J, 1993, MAR ECOL PROG SER, V97, P1, DOI 10.3354/meps097001; BORSHEIM KY, 1990, APPL ENVIRON MICROB, V56, P352; BRATBAK G, 1992, MAR ECOL PROG SER, V83, P273, DOI 10.3354/meps083273; BRATBAK G, 1994, MICROBIAL ECOL, V28, P209, DOI 10.1007/BF00166811; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; BRUSSAARD CPD, 1995, MAR ECOL PROG SER, V123, P259, DOI 10.3354/meps123259; Chen F, 1996, VIROLOGY, V219, P170, DOI 10.1006/viro.1996.0234; CHEN F, 1995, APPL ENVIRON MICROB, V61, P1274, DOI 10.1128/AEM.61.4.1274-1278.1995; CHEN F, 1995, BIOTECHNIQUES, V18, P609; Chen F, 1996, APPL ENVIRON MICROB, V62, P2869, DOI 10.1128/AEM.62.8.2869-2874.1996; CHISHOLM SW, 1988, NATURE, V334, P340, DOI 10.1038/334340a0; COOMBS DH, 1977, J MOL BIOL, V116, P375, DOI 10.1016/0022-2836(77)90076-6; COTTRELL MT, 1991, MAR ECOL PROG SER, V78, P1, DOI 10.3354/meps078001; COTTRELL MT, 1995, APPL ENVIRON MICROB, V61, P3088, DOI 10.1128/AEM.61.8.3088-3091.1995; FRANK H, 1987, HELGOLANDER MEERESUN, V41, P385, DOI 10.1007/BF02365400; Fuhrman JA, 1995, LIMNOL OCEANOGR, V40, P1236, DOI 10.4319/lo.1995.40.7.1236; Genetic Computer Group, 1994, PROGR MAN WISC PACK; Giovannoni SJ, 1995, NATO ADV SCI INST SE, V38, P217; Guillard R.R.L., 1975, CULTURE PHYTOPLANKTO, P29, DOI 10.1007/978-1-4615-8714-9_3.; HELDAL M, 1991, MAR ECOL PROG SER, V72, P205, DOI 10.3354/meps072205; HENNES KP, 1995, APPL ENVIRON MICROB, V61, P3623, DOI 10.1128/AEM.61.10.3623-3627.1995; HENNES KP, 1995, LIMNOL OCEANOGR, V40, P1054; HIRONS GT, 1994, CYTOMETRY, V15, P129, DOI 10.1002/cyto.990150206; HSIAO CL, 1978, VIROLOGY, V91, P26, DOI 10.1016/0042-6822(78)90352-5; HU NT, 1981, VIROLOGY, V114, P236, DOI 10.1016/0042-6822(81)90269-5; Jacobsen A, 1996, J PHYCOL, V32, P923, DOI 10.1111/j.0022-3646.1996.00923.x; JIANG SC, 1995, APPL ENVIRON MICROB, V61, P317, DOI 10.1128/AEM.61.1.317-325.1995; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; LESER TD, 1995, J MICROBIOL METH, V22, P249, DOI 10.1016/0167-7012(95)00010-I; LISS A, 1981, INTERVIROLOGY, V15, P71, DOI 10.1159/000149216; Maniatis T., 1982, MOL CLONING LAB MANU; MARUSICH EI, 1989, NUCLEIC ACIDS RES, V17, P7514, DOI 10.1093/nar/17.18.7514; MESSING J, 1977, P NATL ACAD SCI USA, V74, P3642, DOI 10.1073/pnas.74.9.3642; Moller A, 1997, BIOTECHNIQUES, V22, P512, DOI 10.2144/97223rr02; Monod C, 1997, J MOL BIOL, V267, P237, DOI 10.1006/jmbi.1996.0867; PALENIK B, 1994, APPL ENVIRON MICROB, V60, P3212, DOI 10.1128/AEM.60.9.3212-3219.1994; PALENIK B, 1992, NATURE, V355, P265, DOI 10.1038/355265a0; PROCTOR LM, 1990, NATURE, V343, P60, DOI 10.1038/343060a0; QUEEN C, 1984, NUCLEIC ACIDS RES, V12, P581, DOI 10.1093/nar/12.1Part2.581; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; Steward GF, 1996, MAR ECOL PROG SER, V131, P287, DOI 10.3354/meps131287; Steward Grieg F., 1992, Marine Microbial Food Webs, V6, P57; Steward Grieg F., 1992, Marine Microbial Food Webs, V6, P79; SUTTLE CA, 1994, MICROBIAL ECOL, V28, P237, DOI 10.1007/BF00166813; SUTTLE CA, 1994, APPL ENVIRON MICROB, V60, P3167, DOI 10.1128/AEM.60.9.3167-3174.1994; SUTTLE CA, 1993, MAR ECOL PROG SER, V92, P99, DOI 10.3354/meps092099; URBACH E, 1992, NATURE, V355, P267, DOI 10.1038/355267a0; VANBOEKEL WHM, 1992, MAR ECOL PROG SER, V81, P269, DOI 10.3354/meps081269; VANDRIEL R, 1978, J MOL BIOL, V123, P713, DOI 10.1016/0022-2836(78)90217-6; Waterbury J.B., 1989, BERGEYS MANUAL SYSTE, P1728; WATERBURY JB, 1993, APPL ENVIRON MICROB, V59, P3393, DOI 10.1128/AEM.59.10.3393-3399.1993; Waterbury JB., 1986, CAN B FISH AQUAT SCI, V214, P71; Wilson WH, 1997, AQUAT MICROB ECOL, V13, P95, DOI 10.3354/ame013095; Wilson WH, 1998, ESTUAR COAST SHELF S, V46, P49, DOI 10.1006/ecss.1998.0333; Wilson WH, 1996, J PHYCOL, V32, P506, DOI 10.1111/j.0022-3646.1996.00506.x; WILSON WH, 1993, APPL ENVIRON MICROB, V59, P3736, DOI 10.1128/AEM.59.11.3736-3743.1993; WILSON WH, 1994, THESIS U WARWICK COV; Yanisch-Perron C., 1985, GENE, V33, P109; ZACHAR V, 1993, NUCLEIC ACIDS RES, V21, P2017, DOI 10.1093/nar/21.8.2017	62	117	134	1	10	AMER SOC MICROBIOLOGY	WASHINGTON	1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 USA	0099-2240			APPL ENVIRON MICROB	Appl. Environ. Microbiol.	JUN	1998	64	6					2051	2060					10	Biotechnology & Applied Microbiology; Microbiology	Biotechnology & Applied Microbiology; Microbiology	ZQ805	WOS:000073904800010	9603813				2021-04-07	
J	Schmid, R				Schmid, R			Photosynthesis of Ectocarpus siliculosus in red light and after pulses of blue light at high pH - evidence for bicarbonate uptake	PLANT CELL AND ENVIRONMENT			English	Article						Ectocarpus (Phaeophyta); blue light; bicarbonate uptake; carbon acquisition; pH; photosynthesis	MARINE MACROALGAE; BROWN-ALGAE; INORGANIC CARBON; FAST RESPONSES; SATURATED PHOTOSYNTHESIS; CIRCADIAN RHYTHMICITY; ULVA-LACTUCA; ACQUISITION; STIMULATION; CO2	Pulses of blue light cause stimulation of red light saturated photosynthesis in Ectocarpus siliculosus, because blue light activates the operation of a pathway for inorganic carbon (C-i) acquisition by inducing the mobilization of CO2 from an intermediate metabolite. In the absence of exogenous C-i, photosynthetic rates roughly equal those of CO2 release by respiration. In seawater of pH 9.5 (2.3 mol m(-3) total C-i, but concentrations of free CO2 below 0.2 mmol m(-3)), photosynthesis was clearly above these rates, although they were only approximate to 30% of those in normal seawater (approximate to pH 8), The degree and the time course of the stimulations of photosynthesis by pulses of blue light mere unaltered at high pH. Essentially the same characteristics were found after buffering or in the presence of acetazolamide, an inhibitor of extracellular carbonic anhydrase activity. Therefore, it is concluded that Ectocarpus is able to directly take up HCO3- in addition to CO2 (uptake of CO32- cannot be excluded), The dependence of photosynthesis on C-i at pH 9.5 was biphasic, with C-i below 0.2 mol m(-3) having no effect at all. In C-i-free seawater, the shapes of the stimulations after blue light pulses differed for pH 6, pH 8 and pit 9.5. At low pit, only the fast peak (maximum approximate to 5 min after blue light) was detected, whereas at high pH mainly the slow peak (maximum approximate to 20 min after blue light) was observed, At the intermediate pH 8, both peaks were present. As inhibition of total carbonic anhydrase by ethoxyzolamide brought out the fast peak of the stimulations at pH 9.5 it is concluded that the fast component was due to a transient disequilibrium of an intracellular pool of C-i which, after blue light, was fed by CO2 released from the postulated storage intermediate.	Univ Marburg, Fachbereich Biol Bot, D-35032 Marburg, Germany	Schmid, R (corresponding author), Univ Marburg, Fachbereich Biol Bot, Lahnberge, D-35032 Marburg, Germany.	schmidr@nws.biologie.uni-marburg.de					AXELSSON L, 1995, PLANT CELL ENVIRON, V18, P439, DOI 10.1111/j.1365-3040.1995.tb00378.x; Beer S, 1994, PROGR PHYCOLOGICAL R, V10, P179; Bowes G., 1987, Plant life in aquatic and amphibious habitats., P79; COOK CM, 1986, J EXP BOT, V37, P977, DOI 10.1093/jxb/37.7.977; DONG LF, 1991, OEBALIA S, V17, P49; DRECHSLER Z, 1994, PLANTA, V194, P250, DOI 10.1007/BF01101685; GIORDANO M, 1989, OECOLOGIA, V81, P534, DOI 10.1007/BF00378965; HAGLUND K, 1992, PLANTA, V188, P1, DOI 10.1007/BF00198932; JOHNSTON A M, 1990, British Phycological Journal, V25, P91; JOHNSTON AM, 1991, CAN J BOT, V69, P1123, DOI 10.1139/b91-144; KERBY NW, 1985, ADV BOT RES, V11, P71, DOI 10.1016/S0065-2296(08)60169-X; Larsson C, 1997, EUR J PHYCOL, V32, P49; MABERLY SC, 1992, PLANT CELL ENVIRON, V15, P255, DOI 10.1111/j.1365-3040.1992.tb01480.x; MORONEY JV, 1985, PLANT PHYSIOL, V79, P177, DOI 10.1104/pp.79.1.177; Provasoli L., 1968, CULTURES COLLECTIONS, P63; RAVEN JA, 1985, PLANT CELL ENVIRON, V8, P417, DOI 10.1111/j.1365-3040.1985.tb01677.x; REISKIND JB, 1989, AQUAT BOT, V33, P71, DOI 10.1016/0304-3770(89)90021-1; REISKIND JB, 1991, P NATL ACAD SCI USA, V88, P2883, DOI 10.1073/pnas.88.7.2883; REISKIND JB, 1988, PLANT PHYSIOL, V87, P686, DOI 10.1104/pp.87.3.686; ROTATORE C, 1992, PLANTA, V188, P539, DOI 10.1007/BF00197046; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; Schmid R, 1996, PLANT CELL ENVIRON, V19, P383, DOI 10.1111/j.1365-3040.1996.tb00330.x; Schmid R, 1996, SCI MAR, V60, P115; WEIDNER M, 1975, Z PFLANZENPHYSIOL, V76, P423, DOI 10.1016/S0044-328X(75)80005-5	29	13	15	0	11	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0140-7791			PLANT CELL ENVIRON	Plant Cell Environ.	MAY	1998	21	5					523	529		10.1046/j.1365-3040.1998.00297.x			7	Plant Sciences	Plant Sciences	106BK	WOS:000075109700008					2021-04-07	
J	Santas, R; Korda, A; Lianou, C; Santas, P				Santas, R; Korda, A; Lianou, C; Santas, P			Community responses to UV radiation. I. Enhanced UVB effects on biomass and community structure of filamentous algal assemblages growing in a coral reef mesocosm	MARINE BIOLOGY			English	Article							ULTRAVIOLET-B RADIATION; ULTRA-VIOLET RADIATION; STRATOSPHERIC OZONE; GROWTH; PHOTOSYNTHESIS; MICROALGAE; SURFACE	Four treatments (PAR; PAR + UVA; PAR + UVA + UVB enhanced by 20% from ambient levels; ambient levels of UVB) were performed using a combination of metal halide lights, UV lamps and cutoff filters over developing assemblages of filamentous algae in a coral reef mesocosm. Exposure to enhanced UVB initially reduced the standing crop by 67% as compared to the productivity of the assemblages grown under PAR and PAR + WA. Treatment reversal from PAR to enhanced UVB restricted temporarily the growth of the brown alga Ectocarpus rhodochondroides. While the spores of this species are inhibited by UVB, the sporophytes seem to be capable of adapting to UVB exposure. The effects of ambient UVB levels on biomass production and community composition were less pronounced, while exposure to UVA did not affect productivity or community composition. All effects due to UVB exposure gradually diminished as succession progressed. Community composition and biomass production were fully restored 1 to 2 weeks after the cessation of exposure to enhanced UVB.	OikoTech Inst, GR-16342 Athens, Greece	Santas, R (corresponding author), OikoTech Inst, Kefallenias 50, GR-16342 Athens, Greece.						Adey W.H., 1983, Coral Reefs, V1, P193, DOI 10.1007/BF00571197; ADEY WH, 1987, PHYCOLOGIA, V26, P374, DOI 10.2216/i0031-8884-26-3-374.1; ADEY WH, 1990, DYNAMIC AQUARIA BUIL; AGRAWAL SB, 1992, ENVIRON EXP BOT, V32, P137, DOI 10.1016/0098-8472(92)90038-4; Behrenfeld MJ, 1997, LIMNOL OCEANOGR, V42, P1, DOI 10.4319/lo.1997.42.1.0001; BOTHWELL ML, 1994, SCIENCE, V265, P97, DOI 10.1126/science.265.5168.97; CALDWELL MM, 1979, BIOSCIENCE, V29, P520, DOI 10.2307/1307719; CUTCHIS P, 1974, SCIENCE, V184, P13, DOI 10.1126/science.184.4132.13; DUGGINS DO, 1989, SCIENCE, V245, P555; GROBE CW, 1994, J PHYCOL, V30, P783, DOI 10.1111/j.0022-3646.1994.00783.x; Hader DP, 1997, J PHOTOCH PHOTOBIO B, V37, P66, DOI 10.1016/S1011-1344(96)07338-1; Hader DP, 1996, FEMS MICROBIOL ECOL, V19, P53; Hader DP, 1997, AQUAT BOT, V56, P253, DOI 10.1016/S0304-3770(96)01107-2; HALLDAL P, 1964, PHYSIOL PLANTARUM, V17, P414, DOI 10.1111/j.1399-3054.1964.tb08174.x; HERNDL GJ, 1993, NATURE, V361, P717, DOI 10.1038/361717a0; HOUGHTON RA, 1989, SCI AM, V260, P18; JOKIEL PL, 1984, LIMNOL OCEANOGR, V29, P192, DOI 10.4319/lo.1984.29.1.0192; KELLY JR, 1986, EFFECTS CHANGES STRA, V2; LARKUM AWD, 1993, PHOTOSYNTH RES, V36, P17, DOI 10.1007/BF00018071; LUNING K, 1980, J PHYCOL, V16, P1; MADRONICH S, 1995, AMBIO, V24, P143; Maegawa Miyuki, 1993, Japanese Journal of Phycology, V41, P207; MANN K H, 1972, Memorie dell'Istituto Italiano di Idrobiologia Dott Marco de Marchi, V29, P353; MOLINA MJ, 1992, ACS SYM SER, V483, P24; POLNE M, 1982, ROLE SOLAR ULTRAVIOL, P573; POST A, 1993, AQUAT BOT, V45, P231, DOI 10.1016/0304-3770(93)90023-P; PREZELIN BB, 1994, NATO ASI SER 1, V18, P181; Santas R, 1997, PLANT ECOL, V128, P93, DOI 10.1023/A:1009717226788; Santas R, 1998, MAR BIOL, V131, P163, DOI 10.1007/s002270050307; SANTAS R, 1989, THESIS G WASHINGTON; SCHREIBER RA, 1995, OPHELIA, V42, P335, DOI 10.1080/00785326.1995.10431512; STOLARSKI RS, 1988, SCI AM, V258, P30, DOI 10.1038/scientificamerican0188-30; WOOD WF, 1989, AQUAT BOT, V33, P41, DOI 10.1016/0304-3770(89)90019-3; WOOD WF, 1987, MAR BIOL, V96, P143, DOI 10.1007/BF00394848; *WORLD MET ORG, 1995, 37 WORLD MET ORG; WORREST RC, 1983, PHYSIOL PLANTARUM, V58, P428, DOI 10.1111/j.1399-3054.1983.tb04204.x	36	24	25	0	10	SPRINGER HEIDELBERG	HEIDELBERG	TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY	0025-3162	1432-1793		MAR BIOL	Mar. Biol.	APR	1998	131	1					153	162		10.1007/s002270050306			10	Marine & Freshwater Biology	Marine & Freshwater Biology	ZN136	WOS:000073613500017					2021-04-07	
J	Peters, AF; Burkhardt, E				Peters, AF; Burkhardt, E			Systematic position of the kelp endophyte Laminarionema elsbetiae (Ectocarpales sensu lato, Phaeophyceae) inferred from nuclear ribosomal DNA sequences	PHYCOLOGIA			English	Article							LIFE-HISTORY; SOUTH-AMERICA; LAMINARIALES PHAEOPHYTA; NUCLEOTIDE-SEQUENCES; RNA GENES; DICTYOSIPHONALES; CULTURE; CHLOROPHYTA; ALGAE; RDNA	The recently described microscopic brown alga Laminarionema elsbetiae Kawai et Tokuyama occurs as an endophyte in Laminaria spp. in the northern hemisphere. Based on morphology, cytology, and reproductive characters it is classified in the Ectocarpales. Phylogenetic analyses of partial SSU and ITS sequences of the nuclear ribosomal DNA of Laminarionema elsbetiae and other brown algae support its placement in the clade of 'simple brown algae' (Ectocarpales sensu late), bur it is not closely related to any of the major subgroups currently recognized in the Ectocarpales sensu late or to other kelp endophytes, which belong to the genus Laminariocolax. Comparisons include published (Adenocystis utricularis, Ectocarpus siliculosus, Gononema pectinatum, Laminariocolax tomentosoides, Scytosiphon lomentaria, 'Streblonema' sp,) as well as so far unpublished (Chordaria linearis, Dictyosiphon foeniculaceus, Myriotrichia clavaeformis, Striaria attenuata) sequences of taxa within the Ectocarpales sensu lato. The position of Laminarionema distant from other brown kelp endophytes suggests that endophytism evolved more than once in the Ectocarpales.	Inst Meereskunde, Abt Meeresbot, D-24105 Kiel, Germany; AG Marine Pathol, D-24105 Kiel, Germany; Univ Kiel, Inst Bot, D-24098 Kiel, Germany	Peters, AF (corresponding author), Inst Meereskunde, Abt Meeresbot, Dusternbrooker Weg 20, D-24105 Kiel, Germany.			Peters, Akira/0000-0001-5332-199X			APT KE, 1988, J PHYCOL, V24, P28; ARIZTIA EV, 1991, J PHYCOL, V27, P428, DOI 10.1111/j.0022-3646.1991.00428.x; BAKKER FT, 1995, EUR J PHYCOL, V30, P197, DOI 10.1080/09670269500650981; BHATTACHARYA D, 1992, EVOLUTION, V46, P1801, DOI 10.1111/j.1558-5646.1992.tb01170.x; BURKHARDT E, 1998, IN PRESS J PHYCOLOGY, V34; Ellertsdottir E, 1997, MAR ECOL PROG SER, V146, P135, DOI 10.3354/meps146135; Fritsch FE, 1945, STRUCTURE REPROD ALG; Hamby RK, 1988, PLANT MOL BIOL REP, V6, P179; HORI T, 1971, BOT MAG TOKYO, V84, P231; HORI T, 1972, BOT MAG TOKYO, V85, P125, DOI 10.1007/BF02489509; KAWAI H, 1995, J PHYCOL, V31, P306, DOI 10.1111/j.0022-3646.1995.00306.x; Kawai Hiroshi, 1995, Phycological Research, V43, P185, DOI 10.1111/j.1440-1835.1995.tb00024.x; Kumar S, 1993, MEGA MOL EVOLUTIONAR; OLSEN JL, 1994, J PHYCOL, V30, P729, DOI 10.1111/j.0022-3646.1994.00729.x; Peters A.F., 1987, PROGR PHYCOLOGICAL R, V5, P223; PETERS AF, 1988, BRIT PHYCOL J, V23, P299, DOI 10.1080/00071618800650331; PETERS AF, 1991, REV CHIL HIST NAT, V64, P261; PETERS AF, 1992, BRIT PHYCOL J, V27, P177, DOI 10.1080/00071619200650181; PETERS AF, 1985, HELGOLANDER MEERESUN, V39, P441, DOI 10.1007/BF01987412; PETERS AF, 1992, J PHYCOL, V28, P678, DOI 10.1111/j.0022-3646.1992.00678.x; Peters AF, 1996, NOVA HEDWIGIA, V62, P341; Peters AF, 1997, J PHYCOL, V33, P294, DOI 10.1111/j.0022-3646.1997.00294.x; Peters AF, 1998, PHYCOLOGIA, V37, P106, DOI 10.2216/i0031-8884-37-2-106.1; PETERS AF, 1998, IN PRESS EUROPEAN J, V32; Peters Akira F., 1996, Hydrobiologia, V326-327, P111, DOI 10.1007/BF00047795; SAUNDERS GW, 1993, MAR BIOL, V115, P347, DOI 10.1007/BF00349831; SAUNDERS GW, 1992, J PHYCOL, V28, P544, DOI 10.1111/j.0022-3646.1992.00544.x; SAUNDERS GW, 1995, PHYCOLOGIA, V34, P383, DOI 10.2216/i0031-8884-34-5-383.1; StacheCrain B, 1997, J PHYCOL, V33, P152, DOI 10.1111/j.0022-3646.1997.00152.x; STARFIELD AM, 1993, AI APPLICATIONS, V7, P1; SWOFFORD DL, 1993, PAUP PHYLOGENETIC AN; Tan IH, 1996, J PHYCOL, V32, P112, DOI 10.1111/j.0022-3646.1996.00112.x; Van den Hoeck C, 1993, ALGEN; VANOPPEN MJH, 1993, MAR BIOL, V115, P381, DOI 10.1007/BF00349835; VANOPPEN MJH, 1995, MAR BIOL, V123, P179, DOI 10.1007/BF00350338; White T.J., 1990, PCR PROTOCOLS GUIDE, P315	36	25	26	1	4	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	MAR	1998	37	2					114	120		10.2216/i0031-8884-37-2-114.1			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	102NP	WOS:000074930800006					2021-04-07	
J	Kiirikki, M; Haapamaki, J; Koponen, J; Ruuskanen, A; Sarkkula, J				Kiirikki, M; Haapamaki, J; Koponen, J; Ruuskanen, A; Sarkkula, J			Linking the growth of filamentous algae to the 3D-ecohydrodynamic model of the Gulf of Finland	ENVIRONMENTAL MODELLING & SOFTWARE			English	Article						Ceramium; Baltic Sea; Ectocarpus; ephemeral algae; littoral; macroalgae; Pilayella	NORTHERN BALTIC PROPER; NITROGEN STORAGE; AMMONIUM UPTAKE; MACROALGAE; PHAEOPHYTA; ISOPODA; MARINE; IDOTEA; FUCUS; SEA	One of the most visible symptoms of eutrophication in the Gulf of Finland is blooms of unattached filamentous algae. This decomposing algal biomass causes serious nuisance problems for recreational uses of the coastal zone, particularly when cast ashore. The nutrient availability for these macroscopic filamentous algae is regulated by a superior competitor, the phytoplankton. Nutrients are left for the weaker competitor only when the conditions are not suitable for the growth of phytoplankton. This happens during vertical mixing of the water column. The lack of stratification prevents the formation of phytoplankton blooms, but does not limit the growth of filamentous algae when still growing attached to the bottom. A simple growth model was developed to describe the growth and biomass of filamentous algae. In the absence of suitable nutrient measurements, the model was linked to a 3D-ecohydrodynamic model which generated the nutrient input data. The model was calibrated with three-year monitoring data of filamentous algal biomass at one location. Validation was carried out with one-year monitoring data from an adjacent location. The model was able to describe the number of biomass peaks and their timing with good accuracy. After further development, the filamentous algal model will be used as one of the management tools for the evaluation of the sustainable nutrient load to the Gulf of Finland. (C) 1998 Elsevier Science Ltd. All rights reserved.	Finnish Environm Inst, FIN-00251 Helsinki, Finland; Univ Helsinki, FIN-00014 Helsinki, Finland	Kiirikki, M (corresponding author), Finnish Environm Inst, PL 140, FIN-00251 Helsinki, Finland.	Kiirikki@iki.fi					ANEER G, 1987, MAR BIOL, V94, P163, DOI 10.1007/BF00392928; ANEER G, 1985, CAN J FISH AQUAT SCI, V42, P83, DOI 10.1139/f85-264; BACK S, 1994, P COST 48 SUBGR 3 WO, P18; FRISK T, 1982, HYDROBIOLOGIA, V86, P133, DOI 10.1007/BF00005800; FRISK T, 1980, VESTIALOUS, P24; FUJITA RM, 1985, J EXP MAR BIOL ECOL, V92, P283, DOI 10.1016/0022-0981(85)90100-5; HAAPALA J, 1994, ESTUAR COAST SHELF S, V38, P507, DOI 10.1006/ecss.1994.1035; HEIN M, 1995, MAR ECOL PROG SER, V118, P247, DOI 10.3354/meps118247; KANGAS P, 1982, Acta Botanica Fennica, V118, P1; Kiirikki M, 1997, SARSIA, V82, P259, DOI 10.1080/00364827.1997.10413653; Kiirikki M, 1996, EUR J PHYCOL, V31, P61, DOI 10.1080/09670269600651201; Kiirikki M, 1996, MAR BIOL, V127, P353, DOI 10.1007/BF00942120; Koponen J., 1992, PUBLICATIONS WATER E; KUUSISTO M, 1998, MODELLED PHYTOPLANKT; LIGNELL A, 1987, BOT MAR, V30, P417, DOI 10.1515/botm.1987.30.5.417; MERINO M, 1992, SCIENCE OF THE TOTAL ENVIRONMENT, SUPPLEMENT 1992, P861; Nash JE., 1970, J HYDROL, V10, P282, DOI [10.1016/0022-1694(70)90255-6, DOI 10.1016/0022-1694(70)90255-6]; Niemi A., 1975, ACTA BOT FENN, V105, P1; Norkko A, 1996, MAR ECOL-P S Z N I, V17, P355, DOI 10.1111/j.1439-0485.1996.tb00514.x; Norkko A, 1996, MAR ECOL PROG SER, V131, P143, DOI 10.3354/meps131143; Pedersen MF, 1996, MAR ECOL PROG SER, V142, P261, DOI 10.3354/meps142261; REED RH, 1983, BOT MAR, V26, P409, DOI 10.1515/botm.1983.26.9.409; REICHERT P, 1995, ENVIRON SOFTW, V10, P199, DOI 10.1016/0266-9838(95)00010-I; REICHERT P, 1995, WATER SCI TECHNOL, V31, P135, DOI 10.1016/0273-1223(95)00187-R; SALEMAA H, 1979, OPHELIA, V18, P133, DOI 10.1080/00785326.1979.10425495; SALEMAA H, 1987, OPHELIA, V27, P1; SFRISO A, 1992, ESTUARIES, V15, P517, DOI 10.2307/1352394; THOMAS TE, 1987, J EXP MAR BIOL ECOL, V107, P1, DOI 10.1016/0022-0981(87)90118-3; VOGT H, 1991, MAR ECOL PROG SER, V69, P189, DOI 10.3354/meps069189; WALLENTINUS I, 1984, MAR BIOL, V80, P215, DOI 10.1007/BF02180189; WALLENTINUS I, 1974, MEMORANDA SOC FAUNA, V50, P81; WALLGREN A, 1976, ACTA RADIOL THER PHY, V15, P1	32	15	16	0	12	ELSEVIER SCI LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND	1364-8152			ENVIRON MODELL SOFTW	Environ. Modell. Softw.		1998	13	5-6					503	509		10.1016/S1364-8152(98)00051-6			7	Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences; Water Resources	Computer Science; Engineering; Environmental Sciences & Ecology; Water Resources	155DT	WOS:000077931500012					2021-04-07	
J	Hedvall, O; Moksnes, PO; Pihl, L				Hedvall, O; Moksnes, PO; Pihl, L			Active habitat selection by megalopae and juvenile shore crabs Carcinus maenas: a laboratory study in an annular flume	HYDROBIOLOGIA			English	Article; Proceedings Paper	32nd European Marine Biology Symposium	AUG 16-22, 1997	KRISTINEBERG MARINE RES STN, LYSEKIL, SWEDEN	Goteborg Univ,Marine Res Ctr, Royal Swedish Acad Sci, Swedish Environm Protect Agcy, Swedish Nat Sci Res Council, Swedish Council Forestry & Agr Res, Community Lysekil, Bohus County Council, Provincial Govt Goteborg & Bohus Ian, Goteborg & Bohus Ian Vattenvardsforbund, EU Objective 2 Reg Fyrstad, Munkedals AB, Preem Environm Fdn, AB Volvo Penta, Akzo Nobel Surface Chem AB, Neste Oxo AB, Borealis AB, Hydro plast AB, Vattenfall Generat Serv AB	KRISTINEBERG MARINE RES STN	Carcinus maenas; hydrodynamics; predation; passive deposition; post-settlement movements	LOBSTERS HOMARUS-AMERICANUS; MOBILE EPIBENTHIC FAUNA; ZOSTERA-MARINA; DUNGENESS CRAB; HYDRODYNAMICAL PROCESSES; INTERTIDAL BARNACLE; CALLINECTES-SAPIDUS; SWIMMING BEHAVIOR; CANCER-MAGISTER; PANULIRUS-ARGUS	We studied megalopae (postlarvae) and young juveniles of the shore crab (Carcinus maenas L.) in laboratory experiments to examine four potentially important processes for juvenile distribution and recruitment: (1) hydrodynamic processes and passive deposition of megalopae, (2) active habitat selection of megalopae, (3) habitat specific predation rates, and (4) active habitat selection by juveniles. In an annular flume, simulating natural current velocities in nursery areas on the Swedish west coast, we assessed the distribution of dead megalopae, Live megalopae, live megalopae with predators (juvenile conspecifics and brown shrimp, Crangon crangon), and first instar crabs, in four simultaneously presented habitats: blue mussels (Mytilus edulis), eelgrass (Zostera marina), filamentous green algae (Cladophora sp. and Chaetomorpha linum) and bare sand. In a second experiment we studied the distribution of Live megalopae between four different ephemeral macroalgae with different structural complexity (Ulva lactuca, Enteromorpha sp., Cladophora sp. and Ectocarpus siliculosus). Dead megalopae were evenly distributed between the four habitats, whereas all other treatments showed significantly lower proportions of megalopae and juvenile crabs in the sand habitat (0-2%) compared to the structurally complex habitats (24-40%). The distribution between mussels, eelgrass and filamentous algae of live megalopae in absence of predators did not differ significantly from the hydrodynamical null hypothesis, i.e. distribution of dead megalopae. However, predation increased the proportion of megalopae significantly in the filamentous algae, providing the best refuge from predation of these habitats. First instar crabs showed a significantly different distribution compared to megalopae, with higher proportion in the algal habitat, whereas juvenile predatory crabs were found in significantly higher proportion among mussels. Megalopae selected all four different macroalgae species over open sand, but a significantly lower proportion were found in the algae with the highest structural complexity (Ectocarpus siliculosus; 14%) compared to the other algal species (26-30%). These results indicate that passive deposition have Little influence on the small scale (< 10 s of meters) distribution of shore crab megalopae during normal current velocities, but that active habitat selection by megalopae is the major process responsible for the non-random distribution of megalopae and juvenile shore crabs. The results further suggest that the initial distribution of megalopae between nursery habitats is quickly modified by habitat specific predation rates and size-specific movements and habitat choices by juveniles. The correlation between the habitat choice of megalopae and juvenile crabs, and the refuge value of the examined habitats suggests that habitat specific predation rates is a major selective force behind the behavior of active habitat selection in this species.	Univ Gothenburg, Kristineberg Marine Res Stn, Gothenburg, Sweden	Hedvall, O (corresponding author), Univ Gothenburg, Kristineberg Marine Res Stn, Gothenburg, Sweden.						AMOS CL, 1992, ESTUAR COAST SHELF S, V34, P557, DOI 10.1016/S0272-7714(05)80062-9; BADEN SP, 1984, OPHELIA, V23, P65, DOI 10.1080/00785236.1984.10426605; BARSHAW DE, 1990, FISH B-NOAA, V88, P415; BARSHAW DE, 1994, MAR ECOL PROG SER, V106, P203, DOI 10.3354/meps106203; BELL JD, 1986, J EXP MAR BIOL ECOL, V104, P275; BOTERO L, 1982, Journal of Crustacean Biology, V2, P59, DOI 10.2307/1548113; BUTMAN CA, 1989, J EXP MAR BIOL ECOL, V134, P37, DOI 10.1016/0022-0981(90)90055-H; BUTMAN CA, 1988, NATURE, V333, P771, DOI 10.1038/333771a0; BUTMAN CA, 1987, OCEANOGR MAR BIOL, V25, P113; CARR MH, 1994, ECOLOGY, V75, P1320, DOI 10.2307/1937457; Dittel A, 1996, J EXP MAR BIOL ECOL, V198, P191, DOI 10.1016/0022-0981(96)00003-2; ECKMAN JE, 1983, LIMNOL OCEANOGR, V28, P241, DOI 10.4319/lo.1983.28.2.0241; EGGLESTON DB, 1995, MAR ECOL PROG SER, V124, P9, DOI 10.3354/meps124009; EGGLESTON DB, 1995, ECOL MONOGR, V65, P193, DOI 10.2307/2937137; ERIKSSON S, 1977, J EXP MAR BIOL ECOL, V30, P233, DOI 10.1016/0022-0981(77)90033-8; FERNANDEZ M, 1993, MAR ECOL PROG SER, V92, P171, DOI 10.3354/meps092171; FERNANDEZ M, 1994, ESTUARIES, V17, P271, DOI 10.2307/1352575; GAINES SD, 1987, SCIENCE, V235, P479, DOI 10.1126/science.235.4787.479; Gosselin LA, 1996, MAR ECOL PROG SER, V135, P69, DOI 10.3354/meps135069; HANNAN CA, 1984, LIMNOL OCEANOGR, V29, P1108, DOI 10.4319/lo.1984.29.5.1108; HECK KL, 1980, ESTUARIES, V3, P289, DOI 10.2307/1352084; HERRNKIND WF, 1986, MAR ECOL PROG SER, V34, P23, DOI 10.3354/meps034023; ISAKSSON I, 1992, NETH J SEA RES, V30, P131, DOI 10.1016/0077-7579(92)90052-G; JENSEN KT, 1985, J EXP MAR BIOL ECOL, V89, P157, DOI 10.1016/0022-0981(85)90124-8; JONSSON PR, 1991, MAR ECOL PROG SER, V79, P67, DOI 10.3354/meps079067; Klein Breteler W.C.M., 1976, Netherlands J Sea Res, V10, P354, DOI 10.1016/0077-7579(76)90011-9; MEADOWS PS, 1972, ADV MAR BIOL, V10, P271, DOI 10.1016/S0065-2881(08)60418-6; MENGE BA, 1976, AM NAT, V110, P351, DOI 10.1086/283073; Moksnes PO, 1998, MAR ECOL PROG SER, V166, P211, DOI 10.3354/meps166211; Moksnes PO, 1997, J EXP MAR BIOL ECOL, V215, P157, DOI 10.1016/S0022-0981(97)00052-X; Morgan SG, 1996, MAR ECOL PROG SER, V133, P73, DOI 10.3354/meps133073; MULLINEAUX LS, 1991, MAR BIOL, V110, P93, DOI 10.1007/BF01313096; NOWELL ARM, 1987, OCEANOGR MAR BIOL, V25, P91; Palmer MA, 1996, TRENDS ECOL EVOL, V11, P322, DOI 10.1016/0169-5347(96)10038-0; PAWLIK JR, 1991, SCIENCE, V251, P421, DOI 10.1126/science.251.4992.421; PIHL L, 1985, MAR ECOL PROG SER, V22, P169, DOI 10.3354/meps022169; PIHL L, 1982, J EXP MAR BIOL ECOL, V57, P273, DOI 10.1016/0022-0981(82)90197-6; PIHL L, 1986, NETH J SEA RES, V20, P75, DOI 10.1016/0077-7579(86)90063-3; Ropes J.W., 1968, FISHERY B FISH WILDL, V67, P183; SANCHEZSALAZAR ME, 1987, J EXP MAR BIOL ECOL, V111, P181, DOI 10.1016/0022-0981(87)90054-2; Scherer B., 1981, KIELER MEERESFORSCH, V5, P490; SMITH KN, 1992, J EXP MAR BIOL ECOL, V157, P3, DOI 10.1016/0022-0981(92)90070-Q; SOGARD SM, 1989, J EXP MAR BIOL ECOL, V133, P15, DOI 10.1016/0022-0981(89)90155-X; Sokal R.R., 1995, BIOMETRY, V3; STEIN RA, 1976, ECOLOGY, V57, P751, DOI 10.2307/1936188; TAGHON GL, 1984, LIMNOL OCEANOGR, V29, P64, DOI 10.4319/lo.1984.29.1.0064; WAHLE RA, 1991, MAR ECOL PROG SER, V69, P231, DOI 10.3354/meps069231; WAINRIGHT SC, 1990, MAR ECOL PROG SER, V62, P271, DOI 10.3354/meps062271; WILSON KA, 1987, FISH B-NOAA, V85, P53	49	60	61	0	20	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0018-8158			HYDROBIOLOGIA	Hydrobiologia		1998	375-76						89	100		10.1023/A:1017081611077			12	Marine & Freshwater Biology	Marine & Freshwater Biology	137ZZ	WOS:000076948100009					2021-04-07	
J	Ronnberg, O; Mathiesen, L				Ronnberg, O; Mathiesen, L			Long-term changes in the marine macroalgae of Lagaskar, Aland Sea (N Baltic)	NORDIC JOURNAL OF BOTANY			English	Article							FUCUS-VESICULOSUS; ARCHIPELAGO SEA; EUTROPHICATION; TRENDS; DEPTH	Species composition, abundance and depth distribution of marine macroalgae were studied at Lagskar, the Aland Sea, in August 1993. Altogether, 26 species were identified and the most diverse vegetation, comprising 18 species, was recorded at a depth of 4-7 m. Comparison with the results obtained by Andersson in 1956 indicates that the total number of species had decreased by 6, and that the best developed and most diverse algal vegetation was then recorded at a depth of 10-15 m. In 1993 species such as Aglaothamnion roseum, Audouinella efflorescens, Phyllophora pseudoceranoides, Halopteris scoparia and Sphacelaria plumigera had disappeared, or were not recorded. Sphacelaria arctica, Cladophora rupestris, Stictyosiphon tortilis and Polysiphonia fucoides, in the 1950s important and dominating species, were greatly reduced in number. However, Pilayella littoralis, Ectocarpus siliculosus, Fucus vesioculosus and Rhodomela confervoides had increased. Compared with the drastic phytobenthic changes recorded in many coastal areas of the Baltic, the changes in the offshore Lagskar area are of minor extent.	Abo Akad Univ, Dept Biol, Huso Biol Stn, FIN-20520 Turku, Finland; Aarhus Univ, Dept Plant Ecol, Inst Biol Sci, DK-8240 Risskov, Denmark							Bonsdorff E, 1997, OCEANOL ACTA, V20, P319; Fletcher R.L., 1996, ECOL STUD, V123, P7; Haapala J, 1994, FINNISH MARINE RES, V262, P51; HALLFORS G, 1984, OPHELIA S, V3, P51; *HELCOM, 1990, BALT SEA ENV P, V35; KANGAS P, 1982, Acta Botanica Fennica, V118, P1; KAUTSKY H, 1991, INT REV GES HYDROBIO, V76, P423, DOI 10.1002/iroh.19910760315; KAUTSKY H, 1992, Acta Phytogeographica Suecica, V78, P33; KAUTSKY N, 1986, MAR ECOL PROG SER, V28, P1, DOI 10.3354/meps028001; Kiirikki M, 1996, BOT MAR, V39, P133, DOI 10.1515/botm.1996.39.1-6.133; LISITZIN E, 1957, MERENTUTKIMUSLAITOKS, V175, P1; MATHIESEN L, 1965, MEMORANDA SOC FAUNA, V41, P71; MATHIESEN L, 1974, FAUNA FLORA FENNICA, V49, P5; NEHRING D, 1991, INT REV GES HYDROBIO, V76, P297, DOI 10.1002/iroh.19910760303; Nielsen Ruth, 1995, Acta Botanica Fennica, V155, P1; POGREBOFF S, 1987, FAUNA FLORA FENNICA, V63, P85; RAVANKO O, 1968, Acta Botanica Fennica, V79, P1; RONNBERG O, 1985, ANN BOT FENN, V22, P231; RONNBERG O, 1981, Acta Academiae Aboensis Ser B Mathematica et Physica, V41, P1; RONNBERG O, 1984, OPHELIA S, V3, P189; Sanden P, 1996, LIMNOL OCEANOGR, V41, P346, DOI 10.4319/lo.1996.41.2.0346; Schramm W., 1988, KIELER MEERESFORSCH, V6, P221; SCHRAMM W, 1996, ECOL STUD, V123, P131; SCHULZ S, 1986, OPHELIA S, V4, P249; SEINA A, 1993, CLASSIFICATION MAXIM, V20, P5; SEINA A, 1991, FINNISH MARINE RES, V258, P3; VOGT H, 1991, MAR ECOL PROG SER, V69, P189, DOI 10.3354/meps069189; WAERN MATS, 1952, ACTA PHYTO GEOGR SUECICA, V30, P1; WAERN N, 1965, ACTA PHYTOGEOGR SUEC, V50, P15; WALLENTINUS I, 1981, BALTIC SEA ENV P B, P322; Winterhalter B, 1981, BALTIC SEA, P1; WULFF F, 1994, FINNISH MAR RES, V262, P35	32	15	17	1	11	NORDIC JOURNAL OF BOTANY	COPENHAGEN K	THE SECRETARY BOTANICAL MUSEUM GOTHERSGADE 130, DK-1123 COPENHAGEN K, DENMARK	0107-055X			NORD J BOT	Nord. J. Bot.		1998	18	3					379	384		10.1111/j.1756-1051.1998.tb01894.x			6	Plant Sciences	Plant Sciences	129GQ	WOS:000076455800011					2021-04-07	
J	Maier, I; Muller, DG				Maier, I; Muller, DG			Virus binding to brown algal spores and gametes visualized by DAPI fluorescence microscopy	PHYCOLOGIA			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; INFECTION; FASCICULATUS; PBCV-1; DNA		Univ Konstanz, Fak Biol, D-78457 Konstanz, Germany	Maier, I (corresponding author), Univ Konstanz, Fak Biol, D-78457 Konstanz, Germany.						COLEMAN AW, 1981, J HISTOCHEM CYTOCHEM, V29, P959, DOI 10.1177/29.8.6168681; GROMOV B V, 1981, Archiv fuer Hydrobiologie Supplement, V60, P252; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; Maier I, 1997, J PHYCOL, V33, P838, DOI 10.1111/j.0022-3646.1997.00838.x; MAYER JA, 1979, NATURE, V281, P299, DOI 10.1038/281299a0; MEINTS RH, 1988, VIROLOGY, V164, P15, DOI 10.1016/0042-6822(88)90614-9; MEINTS RH, 1984, VIROLOGY, V138, P341, DOI 10.1016/0042-6822(84)90358-1; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, PROTOPLASMA, V193, P58, DOI 10.1007/BF01276634; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; MULLER DG, 1996, HYDROBIOLOGIA, V21, P21; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561	14	7	7	1	7	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	JAN	1998	37	1					60	63		10.2216/i0031-8884-37-1-60.1			4	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	102NV	WOS:000074931400009					2021-04-07	
J	Kapp, M				Kapp, M			Viruses infecting marine brown algae	VIRUS GENES			English	Article						algal virus; brown algae; DNA viruses; Phycodnaviridae; marine algae; brown algae; Ectocarpus; Feldmannia; Myriotrichia; Hincksia	ECTOCARPUS-SILICULOSUS VIRUS; AUREOCOCCUS-ANOPHAGEFFERENS; MICROMONAS-PUSILLA; EUKARYOTIC ALGAE; DNA VIRUSES; GENOME; PHAEOPHYCEAE; PHYTOPLANKTON; FASCICULATUS; MICROALGAE	Viruses infecting algal hosts possess large double-stranded DNA as genomes. We have recently identified a family of viruses specific for filamentous brown algae. In contrast to the better known Chlorella viruses with their lyric infection cycle, marine brown algal viruses latently occur in their host cells and are induced to multiply in response to a variety of external stimuli such as change in light and temperature. Here, I summarize the known properties of this family of viruses and discuss their taxonomic classification.	Univ Konstanz, Fak Biol, D-78457 Constance, Germany	Kapp, M (corresponding author), Univ Konstanz, Fak Biol, D-78457 Constance, Germany.	Mueller@uni-konstanz.de					BERGH O, 1989, NATURE, V340, P467, DOI 10.1038/340467a0; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Chen F, 1996, VIROLOGY, V219, P170, DOI 10.1006/viro.1996.0234; CHEN F, 1995, APPL ENVIRON MICROB, V61, P1274, DOI 10.1128/AEM.61.4.1274-1278.1995; COTTRELL MT, 1991, MAR ECOL PROG SER, V78, P1, DOI 10.3354/meps078001; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; Ivey RG, 1996, VIROLOGY, V220, P267, DOI 10.1006/viro.1996.0314; Kapp Markus, 1997, Phycological Research, V45, P85, DOI 10.1111/j.1440-1835.1997.tb00067.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LEE AM, 1995, VIROLOGY, V212, P474, DOI 10.1006/viro.1995.1505; Maier I, 1997, J PHYCOL, V33, P838, DOI 10.1111/j.0022-3646.1997.00838.x; MARTIN W, 1992, J MOL EVOL, V35, P385, DOI 10.1007/BF00171817; MAYER JA, 1979, NATURE, V281, P299, DOI 10.1038/281299a0; MILLIGAN KLD, 1994, SCIENCE, V266, P805, DOI 10.1126/science.266.5186.805; Mueller D. G., 1996, Protoplasma, V193, P58; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; MULLER DG, 1990, BOT ACTA, V103, P28; PARODI ER, 1994, EUR J PHYCOLOGY, V29; PROCTOR LM, 1990, NATURE, V343, P60, DOI 10.1038/343060a0; REISSER W, 1993, ARCH PROTISTENKD, V143, P257, DOI 10.1016/S0003-9365(11)80293-9; ROHOZINSKI J, 1989, VIROLOGY, V168, P363, DOI 10.1016/0042-6822(89)90277-8; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; SIEBURTH JM, 1988, J PHYCOL, V24, P416, DOI 10.1111/j.1529-8817.1988.tb00192.x; SILVA PAUL C., 1957, MADRONO, V14, P41; SUTTLE CA, 1990, NATURE, V347, P467, DOI 10.1038/347467a0; SUTTLE CA, 1991, APPL ENVIRON MICROB, V57, P721, DOI 10.1128/AEM.57.3.721-726.1991; VALENTIN K, 1990, PLANT MOL BIOL, V15, P575, DOI 10.1007/BF00017832; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	35	9	9	0	8	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0920-8569	1572-994X		VIRUS GENES	Virus Genes		1998	16	1					111	117		10.1023/A:1007962112756			7	Genetics & Heredity; Virology	Genetics & Heredity; Virology	ZC703	WOS:000072608100010	9562895				2021-04-07	
J	Pohnert, G; Boland, W				Pohnert, G; Boland, W			Pericyclic reactions in nature: Synthesis and cope rearrangement of thermolabile bis-alkenylcyclopropanes from female gametes of marine brown algae (Phaeophyceae)	TETRAHEDRON			English	Article							DESMARESTIA-ACULEATA; CUTLERIA-MULTIFIDA; SEXUAL PHEROMONES; BIOSYNTHESIS; HYDROCARBONS; RECEPTOR	The biosyntheses of the 6-substituted cyclohepta-1,4-dienes dictyotene (1), ectocarpene (2), desmarestene (3), vinylcycloheptadiene (4) and lamoxirene (5) involve a spontaneous Cope rearrangement of thermolabile bis-alkenylcyclopropane precursors like 16, 10, 26 and 30. The unstable precursors and the rearranged cycloheptadienes were synthesised from the chiral 2-iodovinylcyclopropane 12 using Pd-0 or Cu-1 catalysed approaches. Activation parameters of the Cope rearrangements were determined. Bioassays with pre-ectocarpene 10 established the thermolabile cyclopropane (1R,2R)-10, rather than the cyclohepta-1,4-diene(6S)-2, as the genuine pheromone of the brown alga Ectocarpus siliculosus. (C) 1997 Elsevier Science Ltd.	UNIV BONN,KEKULE INST ORGAN CHEM & BIOCHEM,D-53121 BONN,GERMANY			Pohnert, Georg/D-3721-2013; Boland, Wilhelm/K-7762-2012	Pohnert, Georg/0000-0003-2351-6336; Boland, Wilhelm/0000-0001-6784-2534			ALEXAKIS A, 1979, J ORGANOMET CHEM, V177, P293, DOI 10.1016/S0022-328X(00)92355-6; BOLAND W, 1982, EUR J BIOCHEM, V126, P173, DOI 10.1111/j.1432-1033.1982.tb06763.x; BOLAND W, 1984, EUR J BIOCHEM, V144, P169, DOI 10.1111/j.1432-1033.1984.tb08445.x; BOLAND W, 1985, EUR J BIOCHEM, V147, P83, DOI 10.1111/j.1432-1033.1985.tb08722.x; BOLAND W, 1995, P NATL ACAD SCI USA, V92, P37, DOI 10.1073/pnas.92.1.37; BOLAND W, 1994, J PRAK CHEM-CHEM ZTG, V336, P714, DOI 10.1002/prac.19943360817; BOLAND W, 1987, HELV CHIM ACTA, V70, P1025, DOI 10.1002/hlca.19870700415; BOLAND W, 1987, BIOL UNSERER ZEIT, V17, P176; BOLAND W, 1995, ANGEW CHEM INT EDIT, V34, P1598; BROWN JM, 1978, J CHEM SOC PERK T 2, P436, DOI 10.1039/p29780000436; CHEMIN D, 1994, TETRAHEDRON, V50, P5335, DOI 10.1016/S0040-4020(01)80691-8; GRANDJEAN D, 1991, TETRAHEDRON, V47, P1215, DOI 10.1016/S0040-4020(01)86378-X; Hudlicky T, 1992, ORG, P1, DOI DOI 10.1002/0471264180.0R041.01; KEITEL J, 1990, HELV CHIM ACTA, V73, P2101, DOI 10.1002/hlca.19900730806; MAIER I, 1993, PLANT CELL ENVIRON, V16, P891, DOI 10.1111/j.1365-3040.1993.tb00513.x; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MAIER I, 1994, Z NATURFORSCH C, V49, P601; MAIER I, 1987, Z NATURFORSCH C, V42, P948; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; MULLER DG, 1976, Z PFLANZENPHYSIOL, V80, P120; MULLER DG, 1982, NATURWISSENSCHAFTEN, V69, P290, DOI 10.1007/BF00396442; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; Normant J. F., 1983, MODERN SYNTHETIC MET, V3, P139; PANTKEBOCKER S, 1995, TETRAHEDRON, V51, P7927, DOI 10.1016/0040-4020(95)00435-B; PANTKEBOCKER S, 1995, THESIS U KARLSRUHE; Pohnert G, 1996, TETRAHEDRON, V52, P10073, DOI 10.1016/0040-4020(96)00548-0; REETZ MT, 1985, CHEM BER-RECL, V118, P1441, DOI 10.1002/cber.19851180413; SCHNEIDER M, 1975, ANGEW CHEM INT EDIT, V14, P707, DOI 10.1002/anie.197507071; STRATMANN K, 1992, ANGEW CHEM INT EDIT, V31, P1246, DOI 10.1002/anie.199212461; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; SWINBOURNE ES, 1960, J CHEM SOC, V473, P2371; TAKAI K, 1986, J AM CHEM SOC, V108, P7408, DOI 10.1021/ja00283a046; VOGEL E, 1965, LIEBIGS ANN CHEM, V682, P1; WIRTH D, 1992, HELV CHIM ACTA, V75, P734, DOI 10.1002/hlca.19920750309	34	17	18	1	18	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB	0040-4020			TETRAHEDRON	Tetrahedron	OCT 6	1997	53	40					13681	13694		10.1016/S0040-4020(97)00886-7			14	Chemistry, Organic	Chemistry	XZ447	WOS:A1997XZ44700012					2021-04-07	
J	Maier, I; Rometsch, E; Wolf, S; Kapp, M; Muller, DG; Kawai, H				Maier, I; Rometsch, E; Wolf, S; Kapp, M; Muller, DG; Kawai, H			Passage of a marine brown algal DNA virus from Ectocarpus fasciculatus (Ectocarpales, Phaeophyceae) to Myriotrichia clavaeformis (Dictyosiphonales, Phaeophyceae): Infection symptoms and recovery	JOURNAL OF PHYCOLOGY			English	Article						DAPI; DNA virus; Ectocarpus; electron microscopy; host specificity; infection experiments; intergeneric virus transfer; Myriotrichia; PCR; reconstitution	SILICULOSUS PHAEOPHYCEAE; GENOME; HOST; CULTURE	A dsDNA virus (EfasV-1) isolated from Ectocarpus fasciculatus Harvey infected Myriotrichia clavaeformis Harvey, a species belonging to a different brown algal order. The virus did not complete its infection cycle in the foreign host but caused infertility due to malformed reproductive structures. After some time in culture, the host's reproductive capacity was sometimes restored with concomitant loss of at least part of the viral genome. This incidence of interordinal virus transfer is discussed in relation to possibilities for virus-mediated horizontal gene transfer in brown algae.	KOBE UNIV,DEPT BIOL,KOBE,HYOGO 657,JAPAN	Maier, I (corresponding author), UNIV KONSTANZ,FAK BIOL,D-78457 CONSTANCE,GERMANY.						AMABILECUEVAS CF, 1993, AM SCI, V81, P332; BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KUHLENKAMP R, 1994, BOT MAR, V37, P525, DOI 10.1515/botm.1994.37.6.525; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MCCLURE MA, 1987, P NATL ACAD SCI USA, V84, P2693, DOI 10.1073/pnas.84.9.2693; Muller DG, 1996, PHYCOLOGIA, V35, P61, DOI 10.2216/i0031-8884-35-1-61.1; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; Muller DG, 1996, BOT MAR, V39, P401, DOI 10.1515/botm.1996.39.1-6.401; MULLER DG, 1990, BOT ACTA, V103, P72; Muller DG, 1996, PROTOPLASMA, V193, P58, DOI 10.1007/BF01276634; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; Muller DG, 1996, J GEN VIROL, V77, P2329, DOI 10.1099/0022-1317-77-9-2329; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; MULLER DG, 1996, HYDROBIOLOGIA, V21, P21; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x	21	9	9	0	3	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	OCT	1997	33	5					838	844		10.1111/j.0022-3646.1997.00838.x			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	YE659	WOS:A1997YE65900017					2021-04-07	
J	Maier, I				Maier, I			The fine structure of the male gamete of Ectocarpus siliculosus (Ectocarpales, Phaeophyceae) .1. General structure of the cell	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						brown algae; Ectocarpus; electron microscopy; motile cell ultrastructure; reproduction; zoid	ALGA PYLAIELLA-LITTORALIS; BROWN ALGA; FLAGELLAR APPARATUS; REPRODUCTIVE STAGES; COATED VESICLES; PLANT-CELLS; ULTRASTRUCTURE; LAMINARIALES; CHRYSOPHYCEAE; ZOOSPORES	The ultrastructure of male gametes of Ectocarpus siliculosus is described and discussed in relation to the information available on other brown algal zoids. In general, the fine structure and the organelle associations agree with those of other brown algal cells. In particular, the male gametes typically contain a single chloroplast with a pyrenoid and a prominent concave eyespot, into which the swelling of the posterior flagellum (the photoreceptor) fits. The continuity of the chloroplast endoplasmic reticulum and nuclear membrane, as well as the presence of a perinuclear Golgi consisting of several dictyosomes, is shown. The fine structure of microbodies, lipid bodies and different types of vesicles, including physodes and coated vesicles, is also discussed. Several specializations of cellular elements and their interactions are unique and have not been reported before. These features include the connection between the nuclear envelope, the Golgi and the flagellar bases mediated by fibres resembling intermediate filaments, and the organization of the Golgi as well as the association of mitochondria and a microtubular flagellar root.		Maier, I (corresponding author), UNIV KONSTANZ, FAK BIOL, POSTFACH 5560, D-78457 CONSTANCE, GERMANY.						ANDERSEN RA, 1992, PROTOPLASMA, V166, P8, DOI 10.1007/BF01320138; ANDERSEN RA, 1985, PROTOPLASMA, V128, P94, DOI 10.1007/BF01276332; BAKER JRJ, 1973, PROTOPLASMA, V77, P181, DOI 10.1007/BF01276756; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BERKALOFF C, 1979, J PHYCOL, V15, P163, DOI 10.1111/j.0022-3646.1979.00163.x; BISALPUTRA T, 1971, J MICROSC-PARIS, V10, P83; BOUCK GB, 1969, J CELL BIOL, V40, P446, DOI 10.1083/jcb.40.2.446; BOUCK GB, 1970, ANN NY ACAD SCI, V175, P673, DOI 10.1111/j.1749-6632.1970.tb45184.x; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; CHEIGNON M, 1964, CR HEBD ACAD SCI, V258, P676; CHI EY, 1972, P 7 INT SEAW S, P181; CLAYTON MN, 1989, SYST ASSOC SPEC VOL, V38, P229; CLAYTON MN, 1994, EUR J PHYCOL, V29, P1, DOI 10.1080/09670269400650411; CLAYTON MN, 1983, J PHYCOL, V19, P4, DOI 10.1111/j.0022-3646.1983.00004.x; CLAYTON MN, 1984, J PHYCOL, V20, P276, DOI 10.1111/j.0022-3646.1984.00276.x; COLEMAN J, 1987, J CELL SCI, V88, P35; DEININGER W, 1995, EMBO J, V14, P5849, DOI 10.1002/j.1460-2075.1995.tb00273.x; Dodge JD, 1973, FINE STRUCTURE ALGAL; EMONS AMC, 1986, EUR J CELL BIOL, V41, P57; FALK H, 1969, J CELL BIOL, V43, P167, DOI 10.1083/jcb.43.1.167; FALK H, 1967, ARCH MIKROBIOL, V58, P212, DOI 10.1007/BF00408805; GHERARDINI GL, 1972, P 7 INT SEAW S, P172; GIBBS SP, 1981, INT REV CYTOL, V72, P49, DOI DOI 10.1016/S0074-7696(08)61194-8; GROSS W, 1990, J PHYCOL, V26, P381, DOI 10.1111/j.0022-3646.1990.00381.x; HANG HX, 1987, CR ACAD SCI III-VIE, V304, P465; HENRY EC, 1982, J PHYCOL, V18, P570; HENRY EC, 1982, J PHYCOL, V18, P550; HIBBERD D J, 1971, British Phycological Journal, V6, P1; Huang A. H. C., 1983, PLANT PEROXISOMES; KATSAROS C, 1986, PHYCOLOGIA, V25, P358, DOI 10.2216/i0031-8884-25-3-358.1; KATSUMOTO T, 1991, J ELECTRON MICROSC, V40, P24; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; Kawai H., 1992, KOREAN J PHYCOLOGY, V7, P33; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KREIMER G, 1994, INT REV CYTOL, V148, P229, DOI 10.1016/S0074-7696(08)62409-2; LACLAIRE JW, 1979, PROTOPLASMA, V101, P247, DOI 10.1007/BF01276967; LACLAIRE JW, 1978, PROTOPLASMA, V97, P93, DOI 10.1007/BF01276686; LARSEN J, 1985, BRIT PHYCOL J, V20, P341, DOI 10.1080/00071618500650351; LOFTHOUSE PF, 1975, PROTOPLASMA, V84, P83, DOI 10.1007/BF02075945; LOISEAUX S, 1973, J PHYCOL, V9, P277; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; MAIER I, 1982, PHYCOLOGIA, V21, P1, DOI 10.2216/i0031-8884-21-1-1.1; Maier I, 1997, EUR J PHYCOL, V32, P255; Maier I, 1995, PHYCOLOGIA, V34, P441, DOI 10.2216/i0031-8884-34-6-441.1; Maier Ingo, 1995, Phycological Research, V43, P33, DOI 10.1111/j.1440-1835.1995.tb00003.x; MANTON I, 1959, J EXP BOT, V10, P448, DOI 10.1093/jxb/10.3.448; MANTON I, 1957, J EXP BOT, V8, P294, DOI 10.1093/jxb/8.2.294; MANTON I, 1956, J EXP BOT, V7, P416, DOI 10.1093/jxb/7.3.416; MANTON I, 1951, J EXP BOT, V2, P242, DOI 10.1093/jxb/2.2.242; MANTON I., 1964, NEW PHYTQL, V63, P244, DOI 10.1111/j.1469-8137.1964.tb07377.x; Manton I., 1956, CELLULAR MECHANISMS, P61; MARKEY DR, 1976, PROTOPLASMA, V88, P175, DOI 10.1007/BF01283244; MARKEY DR, 1976, PROTOPLASMA, V88, P147, DOI 10.1007/BF01283243; MARKEY DR, 1975, PROTOPLASMA, V85, P219, DOI 10.1007/BF01567948; MASSALSKI A, 1969, British Phycological Journal, V4, P159; MOTOMURA T, 1985, Japanese Journal of Phycology, V33, P199; MOTOMURA T, 1988, J PHYCOL, V24, P282; MOTOMURA T, 1994, PROTOPLASMA, V178, P97, DOI 10.1007/BF01545960; MOTOMURA T, 1989, Japanese Journal of Phycology, V37, P105; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1996, HYDROBIOLOGIA, V21, P21; O'KELLY C J, 1989, Cryptogamic Botany, V1, P58; OKELLY CJ, 1985, PHYCOLOGIA, V24, P263, DOI 10.2216/i0031-8884-24-3-263.1; OLIVEIRA I, 1973, J SUBMICROSC CYTOL, V5, P107; OLIVEIRA L, 1976, CAN J BOT, V54, P913, DOI 10.1139/b76-095; OLIVEIRA L, 1980, PROTOPLASMA, V104, P11; PAPENFUSS G. F., 1951, SVENSK BOT TIDSKR, V45, P4; PHILLIPS JA, 1994, PHYCOLOGIA, V33, P415, DOI 10.2216/i0031-8884-33-6-415.1; REIZE IB, 1989, BOT ACTA, V102, P145, DOI 10.1111/j.1438-8677.1989.tb00083.x; SATIATJEUNEMAITRE B, 1993, BIOL CELL, V79, P7, DOI 10.1016/0248-4900(93)90257-F; SCHNEPF E, 1969, PROTOPLASMA, V68, P85, DOI 10.1007/BF01247899; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; STABENAU H, 1992, PHYLOGENETIC CHANGES, P112; SUZUKI K, 1991, J PHYCOL, V27, P492, DOI 10.1111/j.0022-3646.1991.00492.x; TOTH R, 1976, J PHYCOL, V12, P222; TOTH R, 1974, J PHYCOL, V10, P170; VENABLE JH, 1965, J CELL BIOL, V25, P407, DOI 10.1083/jcb.25.2.407; WALNE PL, 1995, J EUKARYOT MICROBIOL, V42, P7, DOI 10.1111/j.1550-7408.1995.tb01533.x; WRIGLEY NG, 1968, J ULTRA MOL STRUCT R, V24, P454, DOI 10.1016/S0022-5320(68)80048-6; WYNNE M J, 1976, Phycologia, V15, P435, DOI 10.2216/i0031-8884-15-3-435.1; ZIMMERMANN B, 1984, PROTISTOLOGICA, V20, P591	82	15	15	0	5	TAYLOR & FRANCIS LTD	ABINGDON	4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	AUG	1997	32	3					241	253					13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	XX880	WOS:A1997XX88000003					2021-04-07	
J	Maier, I				Maier, I			The fine structure of the male gamete of Ectocarpus siliculosus (Ectocarpales, Phaeophyceae) .2. The flagellar apparatus	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						brown algae; cytoskeleton; Ectocarpus; electron microscopy; flagellar apparatus; gamete; motile cell ultrastructure; zoid	LAMINARIALES PHAEOPHYCEAE; ABSOLUTE-CONFIGURATION; ALGAE; CHRYSOPHYCEAE; ZOOSPORES; ULTRASTRUCTURE; CELLS; DICTYOTALES; CENTRIOLE; CYCLE	The fine structure of the flagellar apparatus of male gametes of Ectocarpus siliculosus has been studied in negatively stained whole-mount preparations of isolated flagellar apparatuses and by transmission electron microscopy of embedded whole cells and flagellar apparatuses. Reconstructions from serial sections revealed a single absolute configuration and new structural details of the flagellar apparatus. A set of 5 microtubular roots is associated with the 2 flagellar basal bodies: the major anterior root consisting of 9 microtubules originates at the anterior basal body and loops through the anterior part of the cell. Cytoplasmic microtubules originate from this root at the apex of the cell and splay out in a posterior direction through the cell, thus determining cell shape. A broad microtubular band composed of a 9-membered bypassing root and a 3-membered major posterior root runs across the basal bodies. Two additional flagellar roots, the minor anterior and minor posterior rootlets, consist of a single microtubule each. The proximal end of the posterior basal body is laterally connected to the anterior basal body by a cross-striated connective (deltoid band). The basal bodies are connected to the nucleus by a small rhizoplast and several other fibrous structures are associated with the basal bodies. The results are discussed in relation to earlier observations on flagellar apparatus organization in brown algae.		Maier, I (corresponding author), UNIV KONSTANZ, FAK BIOL, POSTFACH 5560, D-78457 CONSTANCE, GERMANY.						ANDERSEN RA, 1991, PROTOPLASMA, V164, P143, DOI 10.1007/BF01320820; ANDERSEN RA, 1989, NORD J BOT, V8, P653, DOI 10.1111/j.1756-1051.1989.tb01742.x; ANDERSEN RA, 1987, AM J BOT, V74, P337, DOI 10.2307/2443810; ANDERSEN RA, 1991, PROTOPLASMA, V164, P1, DOI 10.1007/BF01320809; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BARR DJS, 1985, CAN J BOT, V63, P138, DOI 10.1139/b85-017; BARR DJS, 1992, CAN J BOT, V70, P2163, DOI 10.1139/b92-267; BEECH PL, 1991, PROTOPLASMA, V164, P23, DOI 10.1007/BF01320812; BERKALOFF C, 1979, J PHYCOL, V15, P163, DOI 10.1111/j.0022-3646.1979.00163.x; BESSEN M, 1980, J CELL BIOL, V86, P446, DOI 10.1083/jcb.86.2.446; BOUCK GB, 1969, J CELL BIOL, V40, P446, DOI 10.1083/jcb.40.2.446; BOUCK GB, 1970, ANN NY ACAD SCI, V175, P673, DOI 10.1111/j.1749-6632.1970.tb45184.x; CHEIGNON M, 1964, CR HEBD ACAD SCI, V258, P676; CLAYTON MN, 1989, SYST ASSOC SPEC VOL, V38, P229; CLAYTON MN, 1984, J PHYCOL, V20, P276, DOI 10.1111/j.0022-3646.1984.00276.x; GELY C, 1986, PROTOPLASMA, V132, P23, DOI 10.1007/BF01275786; HENRY EC, 1982, J PHYCOL, V18, P570; HENRY EC, 1982, J PHYCOL, V18, P550; HIBBERD DJ, 1979, BIOSYSTEMS, V11, P243, DOI 10.1016/0303-2647(79)90025-X; KATSAROS C, 1986, PHYCOLOGIA, V25, P358, DOI 10.2216/i0031-8884-25-3-358.1; KATSAROS CI, 1993, J PHYCOL, V29, P787, DOI 10.1111/j.0022-3646.1993.00787.x; KREIMER G, 1994, INT REV CYTOL, V148, P229, DOI 10.1016/S0074-7696(08)62409-2; LACLAIRE JW, 1979, PROTOPLASMA, V101, P247, DOI 10.1007/BF01276967; Lange BMH, 1996, TRENDS CELL BIOL, V6, P348, DOI 10.1016/0962-8924(96)10033-7; LARSEN J, 1985, BRIT PHYCOL J, V20, P341, DOI 10.1080/00071618500650351; Maier I, 1997, EUR J PHYCOL, V32, P241; Maier I, 1995, PHYCOLOGIA, V34, P441, DOI 10.2216/i0031-8884-34-6-441.1; MANTON I, 1959, J EXP BOT, V10, P448, DOI 10.1093/jxb/10.3.448; MANTON I, 1957, J EXP BOT, V8, P294, DOI 10.1093/jxb/8.2.294; MANTON I, 1950, NATURE, V166, P973, DOI 10.1038/166973a0; MANTON I, 1956, J EXP BOT, V7, P416, DOI 10.1093/jxb/7.3.416; MANTON I, 1951, ANN BOT-LONDON, V15, P461, DOI 10.1093/oxfordjournals.aob.a083292; MANTON I., 1964, NEW PHYTQL, V63, P244, DOI 10.1111/j.1469-8137.1964.tb07377.x; Manton I., 1956, CELLULAR MECHANISMS, P61; MARKEY DR, 1977, J ULTRA MOL STRUCT R, V59, P173, DOI 10.1016/S0022-5320(77)80077-4; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MOTOMURA T, 1988, J PHYCOL, V24, P282; MOTOMURA T, 1989, Japanese Journal of Phycology, V37, P105; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; OKELLY CJ, 1989, SYST ASSOC SPEC VOL, V38, P255; OKELLY CJ, 1984, PROTOPLASMA, V123, P18, DOI 10.1007/BF01283178; OKELLY CJ, 1985, PHYCOLOGIA, V24, P263, DOI 10.2216/i0031-8884-24-3-263.1; PHILLIPS JA, 1994, PHYCOLOGIA, V33, P415, DOI 10.2216/i0031-8884-33-6-415.1; PHILLIPS JA, 1991, PHYCOLOGIA, V30, P205, DOI 10.2216/i0031-8884-30-2-205.1; PREISIG HR, 1989, SYST ASSOC SPEC VOL, V38, P167; SALISBURY JL, 1995, CURR OPIN CELL BIOL, V7, P39; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1	47	11	11	0	3	TAYLOR & FRANCIS LTD	ABINGDON	4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	AUG	1997	32	3					255	266					12	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	XX880	WOS:A1997XX88000004					2021-04-07	
J	Makewicz, A; Gribi, C; Eichenberger, W				Makewicz, A; Gribi, C; Eichenberger, W			Lipids of Ectocarpus fasciculatus (Phaeophyceae). Incorporation of [1-C-14]oleate and the role of TAG and MGDG in lipid metabolism	PLANT AND CELL PHYSIOLOGY			English	Article						Ectocarpus fasciculatus; fatty acids; lipid metabolism; monogalactosyldiacylglycerol; Phaeophyceae; triacylglycerol	BROWN-ALGAE PHAEOPHYCEAE; CHAIN FATTY-ACIDS; FUCUS-SERRATUS; GLYCEROLIPID SYNTHESIS; BETAINE LIPIDS; HIGHER-PLANTS; BIOSYNTHESIS; CHROMATOGRAPHY; GALACTOLIPIDS; PHOSPHOLIPIDS	Lipids and fatty acids of Ectocarpus fasciculatus (Ectocarpales, Phaeophyceae) were analyzed, Major polar lipids are monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinovosyldiacylglycerol (SQDG), diacylglycerylhydroxymethyl-N,N,N-trimethyl-beta-alanine (DGTA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and phosphatidylinositol (PI). Diphosphatidylglycerol (DPG), phosphatidic acid (PA) and phosphatidyl-O-[N-(2-hydroxyethyl)glycine] (PHEG) were also present in small amounts, Nonpolar lipids mainly consist of triacylglycerol (TAG) and diacylglycerol (DAG), Major fatty acids are 16:0, 18:1, alpha 18:3, 18:4, 20:4 and 20:5, The positional distribution of fatty acids showed that molecular species of eukaryotic structure account for 99% in MGDG, 98% in DGDG, 62% in PG and 23% in SQDG, On incubation with [1-C-14]18:1 for 30 min, 33% of the total label was detected in TAG, 16% in PG, 14% in PE, 10% in PC and 8% in MGDG, During 7 days of chase, the label in TAG, PG, PE and PC decreased and simultaneously increased in MGDG up to 41% of the total, In SQDG, labelled fatty acids were found in prokaryotic as well as in eukaryotic molecular species, During the experiment, the label shifted from 18:1 to 18:2, 18:3, 18:4 and, to a minor extent, to 20:4 and 20:5 acids indicating 18:1 to be processed by elongation and/or desaturation, These results suggest TAG to act as a major primary acceptor of exogenous oleate and to be involved in the transfer of fatty acids to MGDG and other polar lipids.		Makewicz, A (corresponding author), UNIV BERN, DEPT CHEM & BIOCHEM, FREIESTR 3, CH-3012 BERN, SWITZERLAND.						ARAO T, 1989, PHYTOCHEMISTRY, V28, P805, DOI 10.1016/0031-9422(89)80119-0; BORCH RF, 1975, ANAL CHEM, V47, P2437, DOI 10.1021/ac60364a037; BROWSE J, 1991, ANNU REV PLANT PHYS, V42, P467, DOI 10.1146/annurev.pp.42.060191.002343; CARON L, 1985, PLANT CELL PHYSIOL, V26, P131; CASSAGNE C, 1994, PROG LIPID RES, V33, P55, DOI 10.1016/0163-7827(94)90009-4; Chapman VJ, 1980, SEAWEEDS THEIR USES; DITTMER JC, 1964, J LIPID RES, V5, P126; EICHENBERGER W, 1993, PHYTOCHEMISTRY, V34, P1323, DOI 10.1016/0031-9422(91)80024-U; EICHENBERGER W, 1995, J PLANT PHYSIOL, V146, P398, DOI 10.1016/S0176-1617(11)81999-1; EICHENBERGER W, 1997, IN PRESS PHYTOCHEMIS; FAHMY AR, 1961, HELV CHIM ACTA, V44, P2022, DOI 10.1002/hlca.19610440727; FISCHER W, 1973, H-S Z PHYSIOL CHEM, V354, P1115, DOI 10.1515/bchm2.1973.354.2.1115; FLEURENCE J, 1994, J APPL PHYCOL, V6, P527, DOI 10.1007/BF02182406; FRENTZEN M, 1986, J PLANT PHYSIOL, V124, P193, DOI 10.1016/S0176-1617(86)80034-7; HARWOOD JL, 1989, ADV BOT RES, V16, P1; HEINZ E, 1967, BIOCHIM BIOPHYS ACTA, V144, P333, DOI 10.1016/0005-2760(67)90162-2; HEINZ E, 1993, SULFUR NUTRITION AND ASSIMILATION IN HIGHER PLANTS, P163; Jensen A, 1979, P INT SEAWEED S, V9, P17; JONES AL, 1987, BIOCHEM SOC T, V15, P482, DOI 10.1042/bst0150482; JONES AL, 1992, PHYTOCHEMISTRY, V31, P3397, DOI 10.1016/0031-9422(92)83693-S; JOYARD J, 1987, BIOCH PLANTS, V9, P215; KHOTIMCHENKO SV, 1995, PHYTOCHEMISTRY, V38, P1411, DOI 10.1016/0031-9422(94)00819-F; Khozin I, 1996, PHYTOCHEMISTRY, V42, P1025, DOI 10.1016/0031-9422(96)00053-2; Kim MK, 1996, PHYTOCHEMISTRY, V43, P49, DOI 10.1016/0031-9422(96)00243-9; MUDD JB, 1987, BIOCH PLANTS, V9, P275; MULLER DG, 1994, EUR J PHYCOL, V29, P219, DOI 10.1080/09670269400650671; MUNIER R, 1951, B SOC CHIM BIOL, V33, P846; OHLROGGE J, 1995, PLANT CELL, V7, P957, DOI 10.1105/tpc.7.7.957; ROUGHAN G, 1984, TRENDS BIOCHEM SCI, V9, P383, DOI 10.1016/0968-0004(84)90220-2; ROUGHAN PG, 1982, ANNU REV PLANT PHYS, V33, P97, DOI 10.1146/annurev.pp.33.060182.000525; SATO N, 1991, PLANT CELL PHYSIOL, V32, P845; SCHNEIDER JC, 1994, J PHYCOL, V30, P594, DOI 10.1111/j.0022-3646.1994.00594.x; SMITH KL, 1984, PHYTOCHEMISTRY, V23, P2469, DOI 10.1016/S0031-9422(00)84077-7; SMITH KL, 1984, BIOCHIM BIOPHYS ACTA, V796, P119, DOI 10.1016/0005-2760(84)90245-5; SOUTH GR, 1987, INTRO PHYCOLOGY, P27; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; STERN N, 1993, BIOCHIM BIOPHYS ACTA, V1167, P248, DOI 10.1016/0005-2760(93)90226-Y; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; THIES W, 1971, Z PFLANZENZUCHTUNG, V65, P181; Vaskovsky VE, 1996, PHYTOCHEMISTRY, V42, P1347, DOI 10.1016/0031-9422(96)00117-3; Williams JP, 1996, PLANT PHYSIOL BIOCH, V34, P93	41	32	36	0	7	OXFORD UNIV PRESS	OXFORD	GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND	0032-0781	1471-9053		PLANT CELL PHYSIOL	Plant Cell Physiol.	AUG	1997	38	8					952	960		10.1093/oxfordjournals.pcp.a029257			9	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	XR158	WOS:A1997XR15800010		Bronze, Green Published			2021-04-07	
J	Del Campo, E; Ramazanov, Z; Garcia-Reina, G; Muller, DG				Del Campo, E.; Ramazanov, Z.; Garcia-Reina, G.; Mueller, D. G.			Photosynthetic responses and growth performance of virus-infected and noninfected Ectocarpus siliculosus (Phaeophyceae)	PHYCOLOGIA			English	Article								The marine brown alga Ectocarpus siliculosus (Dillwyn) Lyngbye can be infected by a double-stranded DNA virus, which is expressed tit the reproductive structures of the hose thalli. The rates of photosynthetic oxygen evolution in infected and uninfected gametophytes and sporophytes were measured at different carbon concentrations to assess a possible detrimental effect of the virus infection. No significant differences were found in photosynthetic and respiratory rates. In addition, biomass production by infected thalli was similar to that of uninfected parent culture. SDS-PAGE protein analysis of infected and uninfected thalli showed no differences in the proteins produced or in the amount of ribulose bisphosphate carboxylase/oxygenase. the key enzyme for CO2 fixation, that was present.	[Del Campo, E.; Ramazanov, Z.; Garcia-Reina, G.] Univ Palmas Gran Canaria, Inst Algol Aplicada, Las Palmas Gran Canaria 35214, Canary Islands, Spain; [Mueller, D. G.] Univ Konstanz, Fak Biol, D-78434 Constance, Germany	Del Campo, E (corresponding author), Univ Palmas Gran Canaria, Inst Algol Aplicada, Muelle Taliarte S-N, Las Palmas Gran Canaria 35214, Canary Islands, Spain.				ECEuropean CommissionEuropean Commission Joint Research Centre [CI1 CT94-0011]; Instituto Tecnologico de Canarias	Gobierno de Canarias is gratefully acknowledged for financial support to Elena del Campo. This research was supported by EC (CI1 CT94-0011) and Instituto Tecnologico de Canarias.	BJORK M, 1992, PLANTA, V187, P152, DOI 10.1007/BF00201637; DUNCAN MJ, 1982, BOT MAR, V25, P445, DOI 10.1515/botm.1982.25.9.445; HAGLUND K, 1992, PLANTA, V187, P275, DOI 10.1007/BF00201951; JEFFREY SW, 1968, BIOL BULL, V135, P149, DOI 10.2307/1539622; LAEMMLI UK, 1970, NATURE, V4, P680, DOI DOI 10.1038/227680A0; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MATTHEWS REF, 1991, PLANT VIROLOGY, P380; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; NAIDU RA, 1986, PHYSIOL MOL PLANT P, V29, P53, DOI 10.1016/S0048-4059(86)80037-6; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; ROBLEDO DR, 1994, EUR J PHYCOL, V29, P247, DOI 10.1080/09670269400650701; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	16	8	8	0	2	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	MAY	1997	36	3					186	189		10.2216/i0031-8884-36-3-186.1			4	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	V50JD	WOS:000203403700002					2021-04-07	
J	Maier, J; Schmid, R				Maier, J; Schmid, R			Inhibition of pteridine biosynthesis eliminates blue-light dependent stimulation of red-light saturated photosynthesis in Laminaria saccharina (L) Lamouroux	JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY			English	Article						Laminaria saccharina (Phaeophyta); blue light; photoreceptor; photosynthesis; pteridine biosynthesis	FAST RESPONSES; BROWN-ALGAE; PHYCOMYCES-BLAKESLEEANUS; ECTOCARPUS PHAEOPHYTA; CIRCADIAN-RHYTHM; DNA PHOTOLYASE; PHOTORECEPTION; THALIANA	Blue-light stimulation of red light-saturated photosynthetic oxygen evolution in Laminaria saccharina (L.) Lamouroux could be abolished within 5 days by incubation in a solution of 2,4-diamino-6-hydroxypyrimidine (DAKP), an inhibitor of GTP-cyclohydrolase I (E.C, 3.5.4.16) activity. Photosynthesis in red light was not detectably affected. GTP-cyclohydrolase I, which catalyses the first step in the biosynthetic pathway of pteridines, was shown to be active in Laminaria. Under conditions that lead to complete inhibition of the photosynthetic stimulation, DAHP reduced the content of the pteridines in the tissue considerably. The amount of pterin was about 14%, that of biopterin was about 45% and that of an unidentified pteridine was about 27% of those of the controls. By contrast, the total concentration of flavins (FAD + FMN - riboflavin) was not significantly affected. The results suggest that pterins may be involved in the response of photosynthesis to blue light, possibly participating in photoreception.	UNIV TUBINGEN,INST CHEM PFLANZENPHYSIOL,D-72076 TUBINGEN,GERMANY; QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND							AHMAD M, 1993, NATURE, V366, P162, DOI 10.1038/366162a0; CORROCHANO LM, 1988, PLANTA, V174, P315, DOI 10.1007/BF00959516; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; GALLAND P, 1983, PHOTOCHEM PHOTOBIOL, V37, P221, DOI 10.1111/j.1751-1097.1983.tb04462.x; GALLAND P, 1992, PHOTOCHEM PHOTOBIOL, V56, P847, DOI 10.1111/j.1751-1097.1992.tb02242.x; GALLAND P, 1988, PHOTOCHEM PHOTOBIOL, V48, P811, DOI 10.1111/j.1751-1097.1988.tb02896.x; GALLAND P, 1987, PHOTOCHEM PHOTOBIOL, V41, P331; HOHL N, 1992, PHOTOCHEM PHOTOBIOL, V55, P239, DOI 10.1111/j.1751-1097.1992.tb04233.x; JOHNSON JL, 1988, P NATL ACAD SCI USA, V85, P2046, DOI 10.1073/pnas.85.7.2046; KAUFMAN LS, 1993, PLANT PHYSIOL, V102, P333, DOI 10.1104/pp.102.2.333; LIN CT, 1995, SCIENCE, V269, P968, DOI 10.1126/science.7638620; MAIER J, 1995, PHOTOCHEM PHOTOBIOL, V61, P206, DOI 10.1111/j.1751-1097.1995.tb03962.x; MAIER J, 1995, PHOTOCHEM PHOTOBIOL, V61, P43, DOI 10.1111/j.1751-1097.1995.tb09241.x; MALHOTRA K, 1995, BIOCHEMISTRY-US, V34, P6892, DOI 10.1021/bi00020a037; NINNEMANN H, 1983, MOL MODELS PHOTORESP, P133; Palmer JM, 1996, PLANT PHYSIOL, V110, P1323, DOI 10.1104/pp.110.4.1323; QUINONES MA, 1994, SCIENCE, V264, P558, DOI 10.1126/science.264.5158.558; SANCAR A, 1994, BIOCHEMISTRY-US, V33, P2, DOI 10.1021/bi00167a001; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Schmid R, 1996, PLANT CELL ENVIRON, V19, P383, DOI 10.1111/j.1365-3040.1996.tb00330.x; SUSIN S, 1994, PLANTA, V193, P514, DOI 10.1007/BF02411556; TILMANS S, 1995, PHOTOCHEM PHOTOBIOL, V62, P588; WARPEHA KMF, 1992, PHOTOCHEM PHOTOBIOL, V55, P595, DOI 10.1111/j.1751-1097.1992.tb04282.x	28	10	11	0	3	ELSEVIER SCIENCE SA LAUSANNE	LAUSANNE	PO BOX 564, 1001 LAUSANNE, SWITZERLAND	1011-1344			J PHOTOCH PHOTOBIO B	J. Photochem. Photobiol. B-Biol.	APR	1997	38	2-3					274	278		10.1016/S1011-1344(96)07464-7			5	Biochemistry & Molecular Biology; Biophysics	Biochemistry & Molecular Biology; Biophysics	XE030	WOS:A1997XE03000029	9203391				2021-04-07	
J	Motomura, T; Ichimura, T; Melkonian, M				Motomura, T; Ichimura, T; Melkonian, M			Coordinative nuclear and chloroplast division in unilocular sporangia of Laminaria angustata (Laminariales, Phaeophyceae)	JOURNAL OF PHYCOLOGY			English	Article						brown algae; centrin; centrioles; centrosome; chloroplast; Laminaria angustata; meiosis; nucleus; unilocular sporangia; zoospore	SP-NOV PHAEOPHYCEAE; LIFE-HISTORY; CENTRIN; IMMUNOFLUORESCENCE; ULTRASTRUCTURE; ECTOCARPUS; IMMUNOLOCALIZATION; ZOOSPOROGENESIS; REPRODUCTION; MICROSCOPE	Changes in the number of nuclei and chloroplasts were examined during the process of unispore formation in unilocular sporangia of Laminaria angustata. Just before meiosis, eight chloroplasts were always present in unilocular sporangial mother cells. The number of chloroplasts remained constant through meiosis. After the resulting four nuclei divided again (third nuclear division), a close association between a nucleus and a chloroplast developed among each of the eight nuclei and eight chloroplasts. The eight chloroplasts divided almost synchronously before the synchronous division of the eight nuclei. Following the 16 nucleate stage with 16 chloroplasts and the final 32 nucleate stage with 32 chloroplasts, 32 unispores, each with a nucleus and a chloroplast, were formed in unilocular sporangia of L. augustata. Immunofluorescence microscopy using an anti-cent-in antibody showed that two anti-centrin-stained structures (as future mitotic poles) occurred adjacent to each of the premitotic four nuclei, and each spot was located near a chloroplast. Therefore, after the third division, each of the eight nuclei established close contact with a chloroplast presumably mediated by the centrosomes.	UNIV COLOGNE, LEHRSTUHL 1, INST BOT, D-50931 COLOGNE, GERMANY	Motomura, T (corresponding author), HOKKAIDO UNIV, FAC SCI, INST ALGOL RES, MURORAN, HOKKAIDO 051, JAPAN.						BAKER JRJ, 1973, PROTOPLASMA, V77, P181, DOI 10.1007/BF01276756; BERKALOFF C, 1979, J PHYCOL, V15, P163, DOI 10.1111/j.0022-3646.1979.00163.x; BISALPUTRA T, 1969, J ULTRA MOL STRUCT R, V29, P151, DOI 10.1016/S0022-5320(69)80061-4; CHI EY, 1971, P 7 INT SEAW S, P181; CLAYTON MN, 1989, SYST ASSOC SPEC VOL, V38, P229; CLAYTON MN, 1986, BRIT PHYCOL J, V21, P371, DOI 10.1080/00071618600650441; COLEMAN AW, 1985, J PHYCOL, V21, P1; EVANS LV, 1965, ANN BOT-LONDON, V29, P541, DOI 10.1093/oxfordjournals.aob.a083971; FRITSCH FE, 1945, STRUCTURE REPRODUCTI, V2; HENRY EC, 1984, PHYCOLOGIA, V23, P419, DOI 10.2216/i0031-8884-23-4-419.1; HENRY EC, 1982, J PHYCOL, V18, P550; HENRY EC, 1983, PHYCOLOGIA, V22, P387, DOI 10.2216/i0031-8884-22-4-387.1; HOHFELD I, 1994, J PHYCOL, V30, P474, DOI 10.1111/j.0022-3646.1994.00474.x; KATSAROS C, 1991, BOT ACTA, V104, P87, DOI 10.1111/j.1438-8677.1991.tb00201.x; KATSAROS C, 1992, PROTOPLASMA, V169, P75, DOI 10.1007/BF01343372; KATSAROS C, 1986, PHYCOLOGIA, V25, P358, DOI 10.2216/i0031-8884-25-3-358.1; KATSAROS CI, 1993, J PHYCOL, V29, P787, DOI 10.1111/j.0022-3646.1993.00787.x; LOISEAUX S, 1973, J PHYCOL, V9, P277; MARKEY DR, 1976, PROTOPLASMA, V88, P147, DOI 10.1007/BF01283243; Melkonian M., 1992, P179; MOTOMURA T, 1993, I ALG RES HOKKAIDO U, V9, P1; MULLER DG, 1985, PHYCOLOGIA, V24, P467, DOI 10.2216/i0031-8884-24-4-467.1; Nakahara H, 1984, SCI PAP I ALGOL RES, V7, P77; NISHIBAYASHI T, 1961, BIOL OKAYAMA U, V7, P126; OHMORI TAKEO, 1967, BIOL J OKAYAMA UNIV, V13, P23; OKELLY CJ, 1989, CHROMOPHYTE ALGAE, P256; OSBORN M, 1982, METHOD CELL BIOL, V24, P97; SALISBURY JL, 1995, CURR OPIN CELL BIOL, V7, P39; SCHIEBEL E, 1995, TRENDS CELL BIOL, V5, P197, DOI 10.1016/S0962-8924(00)88999-0; TOTH R, 1974, J PHYCOL, V10, P170	30	6	7	0	3	WILEY-BLACKWELL	MALDEN	COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA	0022-3646			J PHYCOL	J. Phycol.	APR	1997	33	2					266	271		10.1111/j.0022-3646.1997.00266.x			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	WV154	WOS:A1997WV15400011					2021-04-07	
J	StacheCrain, B; Muller, DG; Goff, LJ				StacheCrain, B; Muller, DG; Goff, LJ			Molecular systematics of Ectocarpus and Kuckuckia (Ectocarpales, Phaeophyceae) inferred from phylogenetic analysis of nuclear- and plastid-encoded DNA sequences	JOURNAL OF PHYCOLOGY			English	Article						biogeography; Ectocarpus; internal spacer (ITS); Kuckuckia; Phaeophyceae; phylogeny; rbcL; rbcS; RUBISCO spacer	SILICULOSUS DILLW LYNGB; CHLOROPHYTA; GAMETOPHYTES; DELINEATION; SPOROPHYTES; ORIGIN	The phylogeny of Ectocarpus and Kuckuckia strains representing widely separated populations from both hemispheres was inferred from sequence analysis of the internal transcribed spacers of the nuclear ribosomal DNA (ITS 1-5.8S-ITS 2) and the spacer region in the plastid-encoded ribulose-bis-phosphate-carboxylase (RUBISCO) cistron (partial rbcL-spacer-partial rbcS). Both sequences resulted in. matching phylogenies, with the RUBISCO spacer region being most informative at the level of genera and species and the internal transcribed spacer sequences at the level of species and populations. Three major clades were formed by strains previously described by morphology and physiology as Kuckuckia, E. fasciculatus, and E. siliculosus, confirming the validity of these taxa. Ectocarpus and Kuckuckia are regarded as sibling taxa with respect to the outgroup species Feldmannia simplex, Hincksia mitchelliae, and Pilayella littoralis. The clade formed by sexual E. siliculosus strains and most asexual Ectocarpus strains was subdivided into several clades that are consistent with geographical races within E. siliculosus. The inferred phylogeny of Ectocarpus corresponds generally with results from cross-fertilization experiments, morphology, and lipid analysis. A hypothesis on the origin and dispersal of E. siliculosus suggests several natural dispersal events during periods of global cooling as well as recent and possibly anthropogenic dispersal events.	UNIV CALIF SANTA CRUZ,DEPT BIOL,SANTA CRUZ,CA 95064; UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY							BAKKER FT, 1992, J PHYCOL, V28, P839, DOI 10.1111/j.0022-3646.1992.00839.x; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; COLEMAN AW, 1994, J PHYCOL, V30, P80, DOI 10.1111/j.0022-3646.1994.00080.x; DESTOMBE C, 1991, CURR GENET, V19, P395, DOI 10.1007/BF00309601; Felsenstein J., 1989, CLADISTICS, V5, P164, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X; GOFF LJ, 1993, J PHYCOL, V29, P381, DOI 10.1111/j.0022-3646.1993.00381.x; GOFF LJ, 1994, J PHYCOL, V30, P521, DOI 10.1111/j.0022-3646.1994.00521.x; Hamel G., 1931, PHEOPHYCEES FRANCE; KISHINO H, 1989, J MOL EVOL, V29, P170, DOI 10.1007/BF02100115; KOOISTRA WHCF, 1993, PHYCOLOGIA, V32, P419, DOI 10.2216/i0031-8884-32-6-419.1; Lyngbye H.C., 1819, TENTAMEN HYDROPHYTOL; Maddison W. P., 1992, MACCLADE ANAL PHYLOG; Muller D. G., 1991, JPN J PHYCOL, V39, P151; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1976, J PHYCOL, V12, P252, DOI 10.1111/j.0022-3646.1976.00252.x; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; MULLER DG, 1980, NATURWISSENSCHAFTEN, V67, P462, DOI 10.1007/BF00405647; MULLER DG, 1994, EUR J PHYCOL, V29, P219, DOI 10.1080/09670269400650671; MULLER DG, 1977, BRIT PHYCOL J, V12, P131; MULLER DG, 1972, PHYCOLOGIA, V11, P11; PEDERSEN P.M., 1984, OPERA BOT, V74, P1; PEDERSEN PM, 1989, NORD J BOT, V9, P443, DOI 10.1111/j.1756-1051.1989.tb01024.x; PROVASOLI L, 1964, P INT SEAWEED S, V4, P9; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; RUSSELL G, 1967, HELGOLAND WISS MEER, V15, P155, DOI 10.1007/BF01618619; Saunders Gary W., 1993, Hydrobiologia, V260-261, P689, DOI 10.1007/BF00049089; STACHE B, 1993, KONSTANZER DISSERTAT, V376; STACHE B, 1990, NATO ASI SERIES G, V22, P173; SWOFFORD DL, 1991, PAUP PHYLOGENETIC AN; THOMAS DN, 1991, BOT ACTA, V104, P26, DOI 10.1111/j.1438-8677.1991.tb00190.x; THOMAS DN, 1991, PHYSIOL PLANTARUM, V83, P281, DOI 10.1034/j.1399-3054.1991.830212.x; VANOPPEN MJH, 1993, MAR BIOL, V115, P381, DOI 10.1007/BF00349835; White T.J., 1990, PCR PROTOCOLS GUIDE, P315	36	77	79	0	5	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	FEB	1997	33	1					152	168		10.1111/j.0022-3646.1997.00152.x			17	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	WL640	WOS:A1997WL64000019					2021-04-07	
J	VanHeerden, PDR; Robertson, BL; DeKock, L				VanHeerden, PDR; Robertson, BL; DeKock, L			Inhibition of Ectocarpus siliculosus infestations with copper chloride in tank cultures of Gracilaria gracilis	JOURNAL OF APPLIED PHYCOLOGY			English	Article						copper chloride; Ectocarpus siliculosus; epiphyte control; Gracilaria gracilis; Haliotes midae; tank culture	CULTIVATION; EPIPHYTISM; GROWTH; ALGAE	This study investigated copper chloride as an inhibitor of infestations of Ectocarpus siliculosus in Gracilaria gracilis (Stackhouse) Steentoft, Irvine and Farnham grown in outdoor tank cultures. Copper at concentrations of 400 and 800 mu g L-1 Cu2+ inhibited E. siliculosus in one experiment. A reduction in the specific growth rates of G. gracilis was found for both concentrations of copper chloride in two experiments. G. gracilis recovered completely within three weeks in one experiment, while no recovery was found in the other. Three and 5 weeks after treatment with low and high concentrations of copper chloride respectively the specific growth rates of G. gracilis was higher than that of the control in one experiment. A feeding experiment was conducted to determine the effect of copper-treated G. gracilis, used as fodder, on the growth rates of Haliotes midae (abalone) of two size classes. An isolated occurrence of growth rate inhibition of H. midae could be demonstrated in both size classes 3 months after the start of the experiment.	UNIV PORT ELIZABETH,DEPT ZOOL,ZA-6000 PORT ELIZABETH,SOUTH AFRICA	VanHeerden, PDR (corresponding author), UNIV PORT ELIZABETH,DEPT BOT,POB 1600,ZA-6000 PORT ELIZABETH,SOUTH AFRICA.						BIRD CJ, 1995, J APPL PHYCOL, V7, P335, DOI 10.1007/BF00004007; BURDIN KS, 1994, BOT MAR, V37, P467, DOI 10.1515/botm.1994.37.5.467; EVANS LV, 1981, BOT MAR, V24, P167, DOI 10.1515/botm.1981.24.4.167; FLETCHER RL, 1995, J APPL PHYCOL, V7, P325, DOI 10.1007/BF00004006; GOODMAN C, 1976, INT BIODETERIOR, V12, P81; HAGLUND K, 1993, J APPL PHYCOL, V5, P271, DOI 10.1007/BF02186230; KUSCHEL FA, 1991, AQUACULTURE, V92, P7, DOI 10.1016/0044-8486(91)90004-Q; LAPOINTE BE, 1978, AQUACULTURE, V15, P185, DOI 10.1016/0044-8486(78)90030-3; LEWIS AG, 1991, AQUACULTURE, V99, P269, DOI 10.1016/0044-8486(91)90247-5; MORRIS O P, 1974, British Phycological Journal, V9, P269	10	5	5	0	8	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0921-8971			J APPL PHYCOL	J. Appl. Phycol.		1997	9	3					255	259		10.1023/A:1007902122803			5	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	YE299	WOS:A1997YE29900007					2021-04-07	
J	Muller, DG; Eichenberger, W				Muller, Dieter G.; Eichenberger, Waldemar			Mendelian genetics in brown algae: inheritance of a lipid defect mutation and sex alleles in Ectocarpus fasciculatus (Ectocarpales, Phaeophyceae)	PHYCOLOGIA			English	Article								Thalli of the filamentous brown alga Ectocarpus fasciculatus Harvey contain the betaine lipid diacylglycerylhydroxymethyltrimethyl-beta-alanine (DGTA). A sporophyte deficient for DGTA produced DGTA-free gametophytes. We performed a reciprocal bifactorial crossing analysis by mating DGTA-free and wild-type gametes; sex factors served as the second allele pair. The DGTA(+) allele is dominant in both types of hybrid sporophytes. Meiotic segregation of the two allele pairs produced all four possible combinations of sex and DGTA characters, thus showing that these genes are chromosomal and unlinked.	[Muller, Dieter G.] Fak Biol Univ, D-78434 Constance, Germany; [Eichenberger, Waldemar] Inst Biochem Univ, CH-3012 Bern, Switzerland	Muller, DG (corresponding author), Fak Biol Univ, D-78434 Constance, Germany.						Fang TC., 1982, ACTA OCEANOLOGIA SIN, V4, P201; Godward MBE, 1966, CHROMOSOMES ALGAE; Lewis RJ, 1996, PHYCOLOGIA, V35, P19, DOI 10.2216/i0031-8884-35-1-19.1; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1970, NATURWISSENSCHAFTEN, V57, P357; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1994, EUR J PHYCOL, V29, P219, DOI 10.1080/09670269400650671; Schreiber E., 1930, PLANTA, V12, P331; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; VANDERMEER JP, 1987, HYDROBIOLOGIA, V151, P49, DOI 10.1007/BF00046104; VANDERSLUIS PJ, 1986, CELL BIOCHEM FUNCT, V4, P1, DOI 10.1002/cbf.290040102	12	6	6	0	12	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	JAN	1997	36	1					79	81		10.2216/i0031-8884-36-1-79.1			3	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	V43TO	WOS:000202957600012					2021-04-07	
J	Kiirikki, M; Lehvo, A				Kiirikki, M; Lehvo, A			Life strategies of filamentous algae in the northern Baltic Proper	SARSIA			English	Article						life history; macroalgae; recruitment; seasonality; settlement	FUCUS-VESICULOSUS; ECOLOGICAL DOMINANCE; MICROSCOPIC FORMS; SEA; REPRODUCTION; PHAEOPHYTA; COMMUNITY; PATTERNS; SEAWEEDS; FINLAND	Short lived filamentous algae are a major component of the rocky-shore macroalgal vegetation of eutrophic waters in the Baltic Sea. They show considerable variation in abundance both seasonally and from year to year. In this study the seasonal pattern of growth and reproduction is documented in six species to outline their life strategies. Five of the species studied were reproductive in summer or autumn, just after a period of active growth. Pilayella littoralis was the only exception. It grew fast in the spring but reproduction was successful only in winter, when only the basal parts of plants were left. The amount of filamentous algae was very low in winter. The only species which survived the winter period as macroscopic filaments was Ceramium tenuicorne. At least three species, Cladophora glomerata, Dictyosiphon foeniculaceus and Ectocarpus siliculosus, relied on microscopic stages to survive the unfavourable winter period. Responses of these filamentous species to variations in their physical environment are discussed.		Kiirikki, M (corresponding author), FINNISH ENVIRONM INST, POB 140, FIN-00251 HELSINKI, FINLAND.						ANDERSSON S, 1994, MAR ECOL PROG SER, V110, P195, DOI 10.3354/meps110195; BACK S, 1993, SARSIA, V78, P265; BAKER JM, 1989, INT S SERIES, P29; CEDERWALL H, 1980, OPHELIA, P287; DAYTON PK, 1975, ECOL MONOGR, V45, P137, DOI 10.2307/1942404; DEVINNY JS, 1978, MAR BIOL, V48, P343, DOI 10.1007/BF00391638; DURIETZ GE, 1930, BOT NOTISER, P421; ELMGREN R, 1989, AMBIO, V18, P326; HAKANSON L., 1981, MANUAL LAKE MORPHOME; HALLFORS G, 1992, TVARMINNE STUDIES, V5, P15; Hallfors G., 1981, BALTIC SEA, P219; HALLFORS G, 1984, OPHELIA S, V3, P51; Heiskanen AS, 1995, HYDROBIOLOGIA, V316, P211, DOI 10.1007/BF00017438; HOFFMANN AJ, 1991, MAR ECOL PROG SER, V79, P185, DOI 10.3354/meps079185; HOFFMANN AJ, 1987, BOT MAR, V30, P151, DOI 10.1515/botm.1987.30.2.151; JUMPPANEN K, 1992, LOUNAIS SUOMEN VESIE, V78, P1; KANGAS P, 1982, Acta Botanica Fennica, V118, P1; KAUTSKY H, 1989, CONTRIBUTIONS ASKO L, V35, P1; KENNELLY SJ, 1987, MAR ECOL PROG SER, V40, P145, DOI 10.3354/meps040145; Kiirikki M, 1996, EUR J PHYCOL, V31, P225, DOI 10.1080/09670269600651421; Kiirikki M, 1996, EUR J PHYCOL, V31, P61, DOI 10.1080/09670269600651201; Kiirikki M, 1996, MAR BIOL, V127, P353, DOI 10.1007/BF00942120; MAKINEN A, 1984, OPHELIA S, V3, P157; MATHIESON AC, 1989, BOT MAR, V32, P419, DOI 10.1515/botm.1989.32.5.419; MEESE RJ, 1992, J EXP MAR BIOL ECOL, V165, P59, DOI 10.1016/0022-0981(92)90289-M; MURRAY SN, 1989, BOT MAR, V32, P457, DOI 10.1515/botm.1989.32.5.457; NIEMI A, 1987, ANN BOT FENN, V24, P333; Niemi A, 1973, ACTA BOT FENN, V100, P1; NORTON TA, 1978, J MAR BIOL ASSOC UK, V58, P527, DOI 10.1017/S0025315400028186; OJEDA FP, 1984, MAR ECOL PROG SER, V19, P83, DOI 10.3354/meps019083; PETERS AF, 1985, HELGOLANDER MEERESUN, V39, P441, DOI 10.1007/BF01987412; Rahm L, 1996, MAR ECOL PROG SER, V130, P221, DOI 10.3354/meps130221; RONNEBERG O, 1992, ACTA PHYTOGEOGR SUEC, V78, P95; ROSEMARIN AS, 1985, VERH INT VEREIN LIMN, V22, P2872; RUSSELL G, 1985, BRIT PHYCOL J, V20, P87, DOI 10.1080/00071618500650111; RUSSELL G, 1988, HELGOLANDER MEERESUN, V42, P243, DOI 10.1007/BF02366044; RUSSELL G, 1983, MAR ECOL PROG SER, V11, P181, DOI 10.3354/meps011181; Santelices B, 1995, MAR ECOL PROG SER, V129, P215, DOI 10.3354/meps129215; SANTELICES B, 1990, OCEANOGR MAR BIOL, V28, P177; WAEM M, 1952, ACTA PHYTOGEOGRAPHIC, V30, P1; Wallentinus I, 1979, CONTR ASKO LAB U STO, V25, P1; WALLENTINUS I, 1974, MEMORANDA SOC FAUNA, V50, P81	42	52	57	0	13	TAYLOR & FRANCIS AS	OSLO	KARL JOHANS GATE 5, NO-0154 OSLO, NORWAY	0036-4827			SARSIA	Sarsia		1997	82	3					259	267		10.1080/00364827.1997.10413653			9	Ecology; Marine & Freshwater Biology	Environmental Sciences & Ecology; Marine & Freshwater Biology	YE016	WOS:A1997YE01600004					2021-04-07	
J	Kiirikki, M; Blomster, J				Kiirikki, M; Blomster, J			Wind induced upwelling as a possible explanation for mass occurrences of epiphytic Etocarpus siliculosus (Phaeophyta) in the northern Baltic Proper	MARINE BIOLOGY			English	Article							LIVING PILAYELLA-LITTORALIS; AMMONIUM UPTAKE; NAHANT BAY; MACROALGAE; NUTRIENT; SEA; MASSACHUSETTS; ISOPODA; IDOTEA; AREA	The quantity of epiphytic filamentous algae varies considerably in macroalgal vegetation along rocky shores of the northern Baltic Proper. The main species responsible for irregular mass occurrences is the summer annual brown alga Ectocarpus siliculosus (Dillw.) Lyngb. In this study, data collected over a 3-yr monitoring period are related to hydrographical parameters. The tideless and brackish Baltic Sea is salinity stratified, and the salinity difference between surface and bottom waters can be used to indicate upwelling events. Mass occurrences developed when the salinity difference was low. These salinity differences explained 87% of the observed increase in E. siliculosus cover. We propose that the slight salinity changes themselves do not affect the growth of E. siliculosus, but rather they can be used as indicators of short nutrient pulses. These nutrient pulses are not detected in standard water sampling, as they are utilised by both pelagic and benthic organisms within a few hours or days. If the proposed mechanism is true, changes in wind speed and direction have an important effect on the nutrient dynamics of littoral algal communities. A higher frequency of SW winds may cause mass occurrences of filamentous algae, which are often believed to indicate eutrophication of an anthropogenic origin.		Kiirikki, M (corresponding author), UNIV HELSINKI, DEPT ECOL & SYSTEMAT, DIV HYDROBIOL, BOX 17 ARKADIANKATU 7, FIN-00014 HELSINKI, FINLAND.			Blomster, Jaanika/0000-0003-1347-7919			BACK S, 1994, P COST 48 SUBGR 3 WO, P18; BAKER JM, 1989, OLSEN INT S, P29; EFRON B, 1992, MONOGR PENNSYLVANIA, V38; FUJITA RM, 1985, J EXP MAR BIOL ECOL, V92, P283, DOI 10.1016/0022-0981(85)90100-5; Grasshoff K., 1976, METHODS SEAWATER ANA; HAAPALA J, 1994, ESTUAR COAST SHELF S, V38, P507, DOI 10.1006/ecss.1994.1035; HAKANSSON L, 1981, MANUAL LAKE MORPHOME; HALLFORS G, 1984, OPHELIA S, V3, P51; HEIN M, 1995, MAR ECOL PROG SER, V118, P247, DOI 10.3354/meps118247; KANGAS P, 1982, Acta Botanica Fennica, V118, P1; KAUTSKY L, 1982, AQUAT BOT, V12, P23, DOI 10.1016/0304-3770(82)90004-3; Kiirikki M, 1996, EUR J PHYCOL, V31, P61, DOI 10.1080/09670269600651201; KUPARINEN J, 1993, ESTUAR COAST SHELF S, V37, P271, DOI 10.1006/ecss.1993.1056; MAKINEN A, 1984, OPHELIA S, V3, P157; MATTHAUS W, 1988, KIELER MEERESFORSCH, V6, P64; MEESE RJ, 1992, J EXP MAR BIOL ECOL, V165, P59, DOI 10.1016/0022-0981(92)90289-M; PREGNALL AM, 1988, MAR ECOL PROG SER, V50, P161, DOI 10.3354/meps050161; RONNBERG O, 1991, Memoranda Societatis pro Fauna et Flora Fennica, V67, P102; Rosenberg R, 1990, AMBIO, V19, P102; SALEMAA H, 1979, OPHELIA, V18, P133, DOI 10.1080/00785326.1979.10425495; SALEMAA H, 1987, OPHELIA, V27, P1; SFRISO A, 1992, ESTUARIES, V15, P517, DOI 10.2307/1352394; SOULSBY PG, 1985, NETH J SEA RES, V19, P257, DOI 10.1016/0077-7579(85)90031-6; TAMMINEN T, 1985, MARINE BIOL POLAR RE, P363; THOMAS TE, 1987, J EXP MAR BIOL ECOL, V107, P1, DOI 10.1016/0022-0981(87)90118-3; VIITASALO M, 1995, J PLANKTON RES, V17, P1857, DOI 10.1093/plankt/17.10.1857; WILCE RT, 1982, PHYCOLOGIA, V21, P336, DOI 10.2216/i0031-8884-21-3-336.1	27	33	36	0	12	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0025-3162			MAR BIOL	Mar. Biol.	DEC	1996	127	2					353	358		10.1007/BF00942120			6	Marine & Freshwater Biology	Marine & Freshwater Biology	WH772	WOS:A1996WH77200019					2021-04-07	
J	Muller, DG; Sengco, M; Wolf, S; Brautigam, M; Schmid, CE; Kapp, M; Knippers, R				Muller, DG; Sengco, M; Wolf, S; Brautigam, M; Schmid, CE; Kapp, M; Knippers, R			Comparison of two DNA viruses infecting the marine brown algae Ectocarpus siliculosus and E-fasciculatus	JOURNAL OF GENERAL VIROLOGY			English	Article							PHAEOPHYCEAE; GENOME; HOST; CULTURE	The marine brown algal genus Ectocarpus contains two species, E. siliculosus and E. fasciculatus, Field populations of both species include plants with infection symptoms caused by DNA viruses, We have established clonal cultures from infected and normal host plants and investigated the properties of the endogenous viruses, Both host species contain virus particles with a hexagonal cross-section and a diameter of ca, 150 nm, The genomes of both virus types consist of double-stranded DNA, approximately 320 kb in size, Restriction digestion with Sfil revealed differences between the two virus genomes, However, PCR experiments suggest that at least one gene, which encodes a major capsid protein, is quite similar in both virus species, In cross-infection experiments the E. siliculosus virus did not initiate an infection cycle in E. fasciculatus. In contrast, the E. fasciculatus virus infected E. siliculosus zoospores. The resulting plants showed aberrant symptoms and produced virus particles which were not infectious, We conclude that the two Ectocarpus species are hosts for different, but closely related viruses.		Muller, DG (corresponding author), UNIV KONSTANZ,FAK BIOL,UNIV STR 10,D-78434 CONSTANCE,GERMANY.						BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; KUHLENKAMP R, 1994, BOT MAR, V37, P525, DOI 10.1515/botm.1994.37.6.525; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1995, PHYCOLOGIA, V35, P61; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x	14	22	24	0	5	SOC GENERAL MICROBIOLOGY	READING	HARVEST HOUSE 62 LONDON ROAD, READING, BERKS, ENGLAND RG1 5AS	0022-1317			J GEN VIROL	J. Gen. Virol.	SEP	1996	77		9				2329	2333		10.1099/0022-1317-77-9-2329			5	Biotechnology & Applied Microbiology; Virology	Biotechnology & Applied Microbiology; Virology	VF330	WOS:A1996VF33000041	8811034	Bronze			2021-04-07	
J	Hardege, JD; Bentley, MG; Beckmann, M; Muller, C				Hardege, JD; Bentley, MG; Beckmann, M; Muller, C			Sex pheromones in marine polychaetes: Volatile organic substances (VOS) isolated from Arenicola marina	MARINE ECOLOGY PROGRESS SERIES			English	Article						Arenicola marina; spawning; pheromones; volatile organic substances	ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; ENVIRONMENTAL-CONTROL; L ANNELIDA; REPRODUCTION; SEPARATION; LUGWORM; ACID	Volatile organic substances (VOS) were extracted from the coelomic fluid of gravid specimens of the lugworm Arenicola marina through the use of using a closed loop stripping technique and analysed via coupled gas chromatography-mass spectroscopy. VOS detectable in coelomic fluid of mature specimens include aldehydes, n-alkanes, methyl-alkanes, terpenes and ketones. The bouquet of compounds is very similar to nereid volatiles that have been recently described as having sex pheromonal activity. One of the major constituents is the ketone 5-methyl-3-heptanone, the sex pheromone of Platynereis dumerilii and Nereis succinea. Behavioural bioassays with gravid specimens produced no evidence that this substance has any pheromonal function in A. marina. Samples taken at monthly intervals showed an increase in the content of VOS in the coelomic fluid of both sexes during maturation with 1 major peak, 2-ethyl-hexanol, representing up to 50 % of the total amount of VOS in spawning male lugworms. Behavioural assays with ripe females established the existence of a chemical cue inducing the burrow irrigation behaviour during which sea water is pumped through the burrows. This behaviour ensures transport of spermatozoa from the water column into the tube inhabited by the female and increases fertilization success by minimising the dilution effects of the incoming tide. The VOS extract obtained from male spawning water had biological activity in eliciting the 'pumping' behaviour in females, with 16 of 24 worms irrigating continuously upon exposure, whilst controls showed no increase of activity, with 'pumping' occurring at 30 to 40 min intervals.	UNIV ST ANDREWS, GATTY MARINE LAB, ST ANDREWS KY16 8LB, FIFE, SCOTLAND; UNIV OLDENBURG, INST CHEM & BIOL MARINE ENVIRONM, D-26160 OLDENBURG, GERMANY			Muller, Carsten/A-7114-2010	Muller, Carsten/0000-0003-0455-7132; Bentley, Matt/0000-0002-6494-2545			AGOSTA WC, 1992, SCI AM LIB SERIES; Albone E. S., 1984, MAMMALIAN SEMIOCHEMI; ANDERSSON G, 1977, J CHEM ECOL, V5, P629; BENTLEY MG, 1992, OCEANOGR MAR BIOL, V30, P443; BENTLEY MG, 1990, BIOL BULL, V178, P1, DOI 10.2307/1541531; BENTLEY MG, 1996, IN PRESS INVERTEBR R; BESTMANN HJ, 1982, NATURWISSENSCHAFTEN, V69, P457, DOI 10.1007/BF00365811; BOILLYMARER Y, 1978, J EXP ZOOL, V205, P119, DOI 10.1002/jez.1402050114; BOLAND W, 1989, Z NATURFORSCH C, V44, P829; BOLAND W, 1989, HELV CHIM ACTA, V72, P1288, DOI 10.1002/hlca.19890720616; COLL JC, 1995, MAR BIOL, V123, P137, DOI 10.1007/BF00350332; DUNCAN A., 1960, PROC ZOOL SOC LONDON, V134, P137; ELLIN RI, 1974, J CHROMATOGR, V100, P137, DOI 10.1016/S0021-9673(00)86048-3; GROB K, 1976, J CHROMATOGR, V117, P285, DOI 10.1016/0021-9673(76)80005-2; HARDEGE JD, 1994, J MAR BIOL ASSOC UK, V74, P903, DOI 10.1017/S0025315400090135; Hardege JD, 1992, THESIS U OLDENBURG; HOWIE DI, 1961, J MAR BIOL ASSOC UK, V41, P127, DOI 10.1017/S0025315400001582; HOWIE DI, 1961, J MAR BIOL ASSOC UK, V41, P771, DOI 10.1017/S0025315400016295; HOWIE DID, 1984, FORTS ZOOL, V29, P247; HOWIE DID, 1959, J MAR BIOL ASSOC UK, V38, P395, DOI 10.1017/S0025315400006172; HOWIE DID, 1963, GEN COMP ENDOCR, V3, P660, DOI 10.1016/0016-6480(63)90100-X; JAENICKE L, 1977, NATURWISSENSCHAFTEN, V64, P69, DOI 10.1007/BF00437346; JAENICKE L, 1982, ANGEW CHEM, V94, P655; JOSEPHSON DB, 1985, J FOOD SCI, V50, P5, DOI 10.1111/j.1365-2621.1985.tb13265.x; MACHLIS L, 1966, BIOCHEMISTRY-US, V5, P2147, DOI 10.1021/bi00871a001; MEIJER L, 1979, DEV GROWTH DIFFER, V21, P303; MULLER DG, 1982, SCIENCE, V218, P1119, DOI 10.1126/science.218.4577.1119; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; NEUMANN C, 1990, EUR J BIOCHEM, V191, P453, DOI 10.1111/j.1432-1033.1990.tb19143.x; PACEY AA, 1992, J EXP BIOL, V173, P165; POMMERVILLE JC, 1988, PLANT PHYSIOL, V88, P139, DOI 10.1104/pp.88.1.139; SHOREY HH, 1977, ANIMAL COMMUNICATION, P117; SNELL TW, 1995, MAR BIOL, V123, P347, DOI 10.1007/BF00353626; THIESSEN DD, 1977, PROGR POLYCOBIOLOGY, V7, P92; WILLIAMS ME, 1996, IN PRESS INVERTEBR R; ZEECK E, 1992, NATURWISSENSCHAFTEN, V79, P182, DOI 10.1007/BF01134439; ZEECK E, 1988, J EXP ZOOL, V246, P285, DOI 10.1002/jez.1402460308	37	20	21	0	4	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0171-8630			MAR ECOL PROG SER	Mar. Ecol.-Prog. Ser.	AUG	1996	139	1-3					157	166		10.3354/meps139157			10	Ecology; Marine & Freshwater Biology; Oceanography	Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography	VG034	WOS:A1996VG03400013		Bronze			2021-04-07	
J	Muller, DG				Muller, DG			Host-virus interactions in marine brown algae	HYDROBIOLOGIA			English	Article; Proceedings Paper	15th International Seaweed Symposium	JAN, 1995	VALDIVIA, CHILE	Int Seaweed Assoc		DNA-virus; Ectocarpus; Feldmannia; Hincksia; pathology; Phaeophyceae	ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; DNA VIRUS; INFECTIONS; GENOME	Ectocarpus-like marine brown algae are frequently parasitized by polyhedric DNA viruses. Infected hosts have been studied in unialgal and axenic cultures, and the present state of knowledge is summarized in regard to stage-specific virus expression, discharge and survival time of virus particles, infection mechanism, association with host's nuclear genome, passage of the virus genome through mitosis and meiosis of the host, suppression of symptoms and spontaneous recovery of infected plants, host specificity and intergeneric transmission, vitality of infected plants, pandemic occurrence of virus infections, molecular data on Ectocarpus and Feldmannia viruses, and algal DNA-viruses as potential vectors for gene transfer. A scheme for the nomenclature of brown algal viruses is proposed.		Muller, DG (corresponding author), UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY.						BERGH O, 1989, NATURE, V340, P467, DOI 10.1038/340467a0; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; Francki R. I. B., 1991, ARCH VIROLOGY S; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; KUHLENKAMP R, 1994, BOT MAR, V37, P525, DOI 10.1515/botm.1994.37.6.525; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LEWIN R, 1982, SCIENCE, V217, P42, DOI 10.1126/science.7089540; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; MULLER DG, 1993, P INT SEAW S, V14, P37; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; REISSER W, 1993, ARCH PROTISTENKD, V143, P257, DOI 10.1016/S0003-9365(11)80293-9; ROBLEDO DR, 1994, EUR J PHYCOL, V136, P247; SAUVAGEAU C, 1896, J BOTANIQUE, V10, P140; SAUVAGEAU C, 1897, J BOT, V11, P66; SCHWYZER M, 1991, GES ZURICH, V136, P113; SILVA PAUL C., 1957, MADRONO, V14, P41; SUTTLE CA, 1991, APPL ENVIRON MICROB, V57, P721, DOI 10.1128/AEM.57.3.721-726.1991; SYVANEN M, 1985, J THEOR BIOL, V112, P333, DOI 10.1016/S0022-5193(85)80291-5; VANEWTTEN JL, 1991, MICROBIOL REV, V55, P86; ZINSMEISTER D, 1994, NATURWISS RUNDSCH, V47, P131	28	8	8	0	5	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0018-8158			HYDROBIOLOGIA	Hydrobiologia	JUL 26	1996	327						21	28					8	Marine & Freshwater Biology	Marine & Freshwater Biology	VG102	WOS:A1996VG10200007					2021-04-07	
J	Klebl, AK; Brautigam, M; Klein, M; Kapp, M; Knippers, R; Muller, DG				Klebl, AK; Brautigam, M; Klein, M; Kapp, M; Knippers, R; Muller, DG			Molecular and biochemical studies of virus infections in two filamentous brown algae	HYDROBIOLOGIA			English	Article; Proceedings Paper	15th International Seaweed Symposium	JAN, 1995	VALDIVIA, CHILE	Int Seaweed Assoc		Ectocarpus; Feldmannia; Phaeophyceae; virus infection	ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; DNA VIRUS; MARINE; GENOME; HOST; PROTEIN	The filamentous marine brown algae Ectocarpus siliculosus and Feldmannia simplex are infected by host specific DNA-viruses. Under Percoll isolation, Ectocarpus siliculosus-virus (EsV)-particles maintained their infective potential. The EsV has a circular genome of dsDNA with a size of 320 kb. A restriction map has been established. The gene of a major coat protein (gp1) was detected in a genomic library and partly sequenced. Using gp1-sequences for polymerase chain reaction (PCR) amplification analysis, EsV-specific sequences could be detected in various symptom-free, clonal cultures of Ectocarpus. The PCR was also used to follow the passage of the virus genome during the meiosis of hosts. A monospecific antibody against recombinant gp1 was used for immunostaining and infection experiments. The Feldmannia simplex-virus (FlexV-1) has a circular genome with a size of 220 kb and a 43% G+C content. FsV-DNA contains methylated bases. 5-methylcytosine (5 mC) makes up 12% of the total cytosines.		Klebl, AK (corresponding author), UNIV KONSTANZ, FAK BIOL, POSTFACH 5560, D-78434 CONSTANCE, GERMANY.						BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; FLATAU E, 1984, MOL CELL BIOL, V4, P2098, DOI 10.1128/MCB.4.10.2098; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; GERSHONI JM, 1985, ANAL BIOCHEM, V146, P59, DOI 10.1016/0003-2697(85)90395-1; GOFF LJ, 1993, J PHYCOL, V29, P381, DOI 10.1111/j.0022-3646.1993.00381.x; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MATAGNE A, 1991, BIOCHEM J, V280, P553, DOI 10.1042/bj2800553; MESHBAH M, 1989, INT J SYST BACTERIOL, V39, P159; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P4350, DOI 10.1073/pnas.76.9.4350; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	18	2	2	0	1	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0018-8158	1573-5117		HYDROBIOLOGIA	Hydrobiologia	JUL 26	1996	327						105	110					6	Marine & Freshwater Biology	Marine & Freshwater Biology	VG102	WOS:A1996VG10200018					2021-04-07	
J	Muller, DG; Schmid, CE				Muller, DG; Schmid, CE			Intergeneric infection and persistence of Ectocarpus virus DNA in Kuckuckia (Phaeophyceae, Ectocarpales)	BOTANICA MARINA			English	Article							SILICULOSUS; GENOME; HOST	Ectocarpoid brown algae are frequently infected by DNA viruses. With a PCR amplification technique that specifically detects Ectocarpus virus DNA we confirmed that the Ectocarpus siliculosus virus can infect Kuckuckia kylinii and persist in this host for several years. The same technique showed that 33 isolates of Kuckuckia from various Atlantic and Pacific coasts were free of Ectocarpus virus DNA. A collection of kuckuckia from a deep water habitat in the Canarian Archipelago showed morphological symptoms of viral infection, which subsided during laboratory culture. These plants did not respond to the amplification of Ectocarpus virus DNA. We conclude, that these plants were affected by an indigenous Kuckuckia virus, which is suppressed by the host under culture conditions.		Muller, DG (corresponding author), UNIV KONSTANZ, FAC BIOL, D-78434 CONSTANCE, GERMANY.						BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; CLAYTON MN, 1989, HDB PROTOCTISTA, P698; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; GOFF LJ, 1993, J PHYCOL, V29, P381, DOI 10.1111/j.0022-3646.1993.00381.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; Muller DG, 1996, PHYCOLOGIA, V35, P61, DOI 10.2216/i0031-8884-35-1-61.1; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1996, IN PRESS P 15 INT SE; MULLER DG, 1996, IN PRESS PROTOPLASMA; PEDERSEN PM, 1989, NORD J BOT, V9, P443, DOI 10.1111/j.1756-1051.1989.tb01024.x; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; White T.J., 1990, PCR PROTOCOLS GUIDE, P315; Wynne M. J., 1982, SYNOPSIS CLASSIFICAT, V1, P115	19	4	4	0	4	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055			BOT MAR	Bot. Marina	JUL	1996	39	4					401	405		10.1515/botm.1996.39.1-6.401			5	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	UY391	WOS:A1996UY39100011					2021-04-07	
J	Wahl, M				Wahl, M			Fouled snails in flow: Potential of epibionts on Littorina littorea to increase drag and reduce snail growth rates	MARINE ECOLOGY PROGRESS SERIES			English	Article						Littorina littorea; drag; epibiosis; epibiosis costs; growth rates; energy budget	MARINE EPIBIOSIS; COMMUNITY; DEFENSES; FORCES; PREY	Epibiosis is one of the closest interspecies associations. The presence of epibionts potentially causes a multitude of beneficial or detrimental effects for the basibiont. It has been shown previously that large epibionts may increase the risk of dislodgement of bivalves. In this study, sublethal effects of epibiont-induced drag increase are investigated. I assessed (1) the effects of common epibiont species (Balanus improvisus, Enteromorpha intestinalis, Ectocarpus sp.) on drag properties of the host (the periwinkle Littorina littorea), and (2) the long-term consequences of drag increase on growth rates of snails living in steady flow. All epibiont species increase drag on the host snail. They do so to unequal extents. This may be due to morphological and hydrodynamic differences among the epibionts. Thus, per unit volume of epibiont, the filamentous alga Ectocarpus sp, has a substantially stronger effect than the barnacles. Synergistic effects on drag increase can be observed in a mixed aufwuchs community. As compared to clean conspecifics, snails bearing artificial epibionts grow 35% more slowly when exposed to moderate, steady flow (8 cm s(-1)) for 5 mo. This difference in growth rates is enhanced when food is limited. I hypothesize that fouled snails coping with higher drag invest more energy into foot activities (muscles and mucus). As a consequence, when food is limited, growth rates decrease in fouled snails.		Wahl, M (corresponding author), CHRISTIAN ALBRECHTS UNIV KIEL, INST ZOOL, D-24098 KIEL, GERMANY.		Wahl, Martin/D-2038-2016	Wahl, Martin/0000-0001-8703-3857			ANSELL AD, 1988, J MAR BIOL ASSOC UK, V68, P219, DOI 10.1017/S0025315400052139; BARKAI A, 1988, SCIENCE, V242, P62, DOI 10.1126/science.242.4875.62; BLOOM SA, 1975, J EXP MAR BIOL ECOL, V17, P311, DOI 10.1016/0022-0981(75)90006-4; DAVIES MS, 1992, 3RD P INT S LITT BIO, P227; DAYTON PK, 1971, ECOL MONOGR, V41, P351, DOI 10.2307/1948498; DENNY MW, 1985, LIMNOL OCEANOGR, V30, P1171, DOI 10.4319/lo.1985.30.6.1171; DUDLEY R, 1985, VELIGER, V28, P6; EKARATNE SUK, 1984, J MAR BIOL ASSOC UK, V64, P183, DOI 10.1017/S0025315400059725; FEIFAREK BP, 1987, J EXP MAR BIOL ECOL, V105, P39, DOI 10.1016/S0022-0981(87)80028-X; GAYLORD B, 1994, ECOL MONOGR, V64, P28; GRENON JF, 1981, J EXP MAR BIOL ECOL, V54, P227; Judge ML, 1988, FUNCT ECOL, V2, P363, DOI 10.2307/2389409; KEMP P, 1984, P NATL ACAD SCI-BIOL, V81, P811, DOI 10.1073/pnas.81.3.811; NOWELL ARM, 1987, OCEANOGR MAR BIOL, V25, P91; PENHALE PA, 1977, J EXP MAR BIOL ECOL, V26, P211, DOI 10.1016/0022-0981(77)90109-5; SAR N, 1987, MICROBIAL ECOL, V13, P193, DOI 10.1007/BF02024997; VANCE RR, 1978, ECOLOGY, V59, P679, DOI 10.2307/1938770; Vogel S, 1981, LIFE MOVING FLUIDS; WAHL M, 1992, MAR ECOL PROG SER, V88, P225, DOI 10.3354/meps088225; WAHL M, 1995, OECOLOGIA, V102, P329, DOI 10.1007/BF00329800; WAHL M, 1989, MAR ECOL PROG SER, V58, P175, DOI 10.3354/meps058175; WEISSENBERGER J, 1991, FRESHWATER BIOL, V25, P21, DOI 10.1111/j.1365-2427.1991.tb00469.x; WITMAN JD, 1984, MAR ECOL PROG SER, V16, P259, DOI 10.3354/meps016259	23	49	49	0	11	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0171-8630			MAR ECOL PROG SER	Mar. Ecol.-Prog. Ser.	JUL	1996	138	1-3					157	168		10.3354/meps138157			12	Ecology; Marine & Freshwater Biology; Oceanography	Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography	VC147	WOS:A1996VC14700016		Bronze, Green Accepted			2021-04-07	
J	Ivey, RG; Henry, EC; Lee, AM; Klepper, L; Krueger, SK; Meints, RH				Ivey, RG; Henry, EC; Lee, AM; Klepper, L; Krueger, SK; Meints, RH			A Feldmannia algal virus has two genome size-classes	VIROLOGY			English	Article							ECTOCARPUS-SILICULOSUS; DNA VIRUS; MARINE; PHAEOPHYCEAE; INFECTION; HOST	Persistent viruses occur intracellularly in brown algae, specifically the Ectocarpales, and as reported here in the genus Feldmannia. Feldmannia species are small (1 mm-several cm), filamentous forms with single-celled meiotic sporangia that normally produce haploid zoospores. In the isolate reported here, spores were not observed in the sporangia but rather numerous (similar to 10(6) per cell) polyhedral viruses are formed in their place. Two dsDNA genome classes of 158 and 178 kbp, with two restriction site variants of each, are described. The individual abundance of each genome in viral preparations is affected by culture temperature. A cosmid library was used to generate circular restriction enzyme (BamHI, Notl, and Pstl)Site maps. (C) 1996 Academic Press, Inc.	OREGON STATE UNIV,CTR GENE RES & BIOTECHNOL,CORVALLIS,OR 97331; OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,CORVALLIS,OR 97331							BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; CLITHEROE SB, 1974, J ULTRA MOL STRUCT R, V49, P211, DOI 10.1016/S0022-5320(74)80032-8; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; Fuhrman J., 1993, OCEANOGRAPHY, V6, P51, DOI 10.5670/oceanog.1993.14.; GIRTON LE, 1987, PLANT MOL BIOL, V9, P247, DOI 10.1007/BF00166461; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; JIA Y, 1995, UNPUB CHARACTERIZATI; KLEIN M, 1993, VIROLOGY, V202, P1076; Krueger SK, 1996, VIROLOGY, V219, P301, DOI 10.1006/viro.1996.0251; LACLAIRE JW, 1977, PROTOPLASMA, V93, P127, DOI 10.1007/BF01276287; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LEE AM, 1995, VIROLOGY, V212, P474, DOI 10.1006/viro.1995.1505; LEVENE SD, 1987, P NATL ACAD SCI USA, V84, P4054, DOI 10.1073/pnas.84.12.4054; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; ROHOZINSKI J, 1989, VIROLOGY, V168, P363, DOI 10.1016/0042-6822(89)90277-8; Sambrook J., 1989, MOL CLONING LAB MANU; TARTOF KD, 1988, GENE, V67, P169, DOI 10.1016/0378-1119(88)90394-0; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	24	16	16	0	2	ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495	0042-6822			VIROLOGY	Virology	JUN 15	1996	220	2					267	273		10.1006/viro.1996.0314			7	Virology	Virology	UT252	WOS:A1996UT25200002	8661377				2021-04-07	
J	Schmid, R; Dring, MJ				Schmid, R; Dring, MJ			Blue light and carbon acquisition in brown algae: An overview and recent developments	SCIENTIA MARINA			English	Article; Proceedings Paper	II Algal Photobiology Workshop	OCT 10-14, 1994	MALAGA, SPAIN	Minist Educ & Sci Spain, Direccion Gen Univ & Investigac, Univ Malaga		Phaeophyta; blue light; photosynthesis; oxygen evolution; CO2 uptake; carbon acquisition; CAM metabolism	INORGANIC CARBON; MARINE MACROALGAE; SATURATED PHOTOSYNTHESIS; ASCOPHYLLUM-NODOSUM; FAST RESPONSES; ECTOCARPUS PHAEOPHYTA; CIRCADIAN-RHYTHM; BUFFER SYSTEM; ASSIMILATION; STIMULATION	Photosynthesis of brown algae in saturating irradiances of red light is stimulated by blue light. Short pulses of blue light lead to transient increases of photosynthetic rates which, in some species, can be up to 4 times the rates in red light. We can distinguish two groups of species that differ with respect to the pattern of their photosynthetic behaviour in red light and the kinetics of blue-light stimulation. The first group contains the orders of the Phaeophyta with little tissue organisation, and the second group those orders that have tissue organisation. In both groups, blue light is thought to activate a carbon acquisition mechanism because (i) the responses to blue light are abolished with increased supply of free CO2, whereas photosynthetic rates in red light are enhanced, and (ii) the affinity for dissolved inorganic carbon (DIC) is higher in the presence of blue light. The activating mechanism in Group II requires the activity of an extracellular carbonic anhydrase. If DIC is absent from the medium, photosynthesis in species from Group I can still be stimulated by blue light and stimulation in Group II species occurs without the simultaneous enhancement of the rate of uptake of CO2. We suggest, therefore, that blue light activates the release of CO2 from an Internal store. Possible metabolic pathways for the carbon acquisition systems in both groups of species are presented and a CAM-like metabolism is suggested. In Group II species, the carbon store is thought to be located in tissues separate from the photosynthetic tissues. More recent investigations indicate that the Fucales differ from the rest of the Phaeophyta by additional blue-light activation of the loading of the internal CO2 store. The mechanism of activation appears to be different under submersed and emersed conditions.	QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND	Schmid, R (corresponding author), UNIV MARBURG,FACHBEREICH BIOL BOT,D-35032 MARBURG,GERMANY.		Dring, Matthew/B-4941-2014	Dring, Matthew/0000-0001-9043-5670			AKAGAWA H, 1972, PLANT CELL PHYSL, V13, P199; AXELSSON L, 1988, MAR BIOL, V97, P295, DOI 10.1007/BF00391315; AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P771, DOI 10.1111/j.1365-3040.1989.tb01638.x; AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P765, DOI 10.1111/j.1365-3040.1989.tb01637.x; Axelsson L., 1991, SEAWEED CELLULAR BIO, P185; Beer Sven, 1994, V10, P179; Bowes G., 1987, Plant life in aquatic and amphibious habitats., P79; COOK CM, 1986, J EXP BOT, V37, P977, DOI 10.1093/jxb/37.7.977; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; GRIFFITHS H, 1988, ADV BOT RES, V15, P43; HAGLUND K, 1992, PLANTA, V188, P1, DOI 10.1007/BF00198932; JHNSTON AM, 1990, BR PHYCOL J, V25, P91; JOHNSTON AM, 1986, OECOLOGIA, V69, P288, DOI 10.1007/BF00377636; JOHNSTON AM, 1991, CAN J BOT, V69, P1123, DOI 10.1139/b91-144; JOHNSTON AM, 1987, PHYCOLOGIA, V26, P159, DOI 10.2216/i0031-8884-26-2-159.1; JOHNSTON AM, 1989, BR PHYCOL J, V24, P307; JOHNSTON AM, 1987, J PHYCOL, V22, P78; KERBY NW, 1985, ADV BOT RES, V11, P71, DOI 10.1016/S0065-2296(08)60169-X; Kremer B, 1981, OCEANOGR MAR BIOL AN, V19, P41; Kremer B. P., 1981, BIOL SEAWEEDS, P493; Leegood RC, 1990, PLANT PHYSL BIOCH MO, P274; LUCAS WJ, 1983, ANNU REV PLANT PHYS, V34, P71, DOI 10.1146/annurev.pp.34.060183.000443; MABERLY SC, 1990, J PHYCOL, V26, P439, DOI 10.1111/j.0022-3646.1990.00439.x; MADSEN TV, 1991, AQUAT BOT, V41, P5, DOI 10.1016/0304-3770(91)90037-6; MORONEY JV, 1985, PLANT PHYSIOL, V79, P177, DOI 10.1104/pp.79.1.177; RAVEN JA, 1989, PHYCOLOGIA, V28, P429, DOI 10.2216/i0031-8884-28-4-429.1; RAVEN JA, 1985, PLANT CELL ENVIRON, V8, P417, DOI 10.1111/j.1365-3040.1985.tb01677.x; SANCHO A, 1989, PLANT PHYSIOL BIOCH, V27, P537; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; SCHMID R, 1996, IN PRESS PLANT CELL; SURIF MB, 1989, OECOLOGIA, V78, P97, DOI 10.1007/BF00377203; WILKINS MB, 1992, NEW PHYTOL, V121, P347, DOI 10.1111/j.1469-8137.1992.tb02936.x	38	21	22	0	4	INST CIENCIAS MAR BARCELONA	BARCELONA	PASSEIG JOAN DE BORBO, 08039 BARCELONA, SPAIN	0214-8358			SCI MAR	Sci. Mar.	MAY	1996	60			1			115	124					10	Marine & Freshwater Biology	Marine & Freshwater Biology	UZ478	WOS:A1996UZ47800015					2021-04-07	
J	Krueger, SK; Ivey, RG; Henry, EC; Meints, RH				Krueger, SK; Ivey, RG; Henry, EC; Meints, RH			A brown algal virus genome contains a ''RING'' zinc finger motif	VIROLOGY			English	Article							DNA-REPLICATION; PROTEIN; PHAEOPHYCEAE; INITIATION; ECTOCARPUS; INFECTION	The brown filamentous alga Feldmannia sp. contains a large icosahedral dsDNA virus, FsV, of which there are multiple variants. A 4.5-kb SstI-HindIII fragment (SH4.5) that is conserved among all genome variants was sequenced. Three open reading frames (ORF-1, -2, and -3, containing 555, 2022, and 411 bp, respectively) were shown to be transcriptionally active by ribonuclease protection assay. A ''RING'' zinc finger motif and a nucleotide binding site motif were identified in ORF-2. (C) 1996 Academic Press, Inc.	OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,CORVALLIS,OR 97331							Chang S. J., 1993, Plant Molecular Biology Reporter, V11, P113, DOI 10.1007/BF02670468; HENIKOFF S, 1984, GENE, V28, P351, DOI 10.1016/0378-1119(84)90153-7; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; IVEY RG, UNPUB; KOONIN EV, 1993, NUCLEIC ACIDS RES, V21, P2541, DOI 10.1093/nar/21.11.2541; KOZAK M, 1983, MICROBIOL REV, V47, P1; LEE AM, 1995, VIROLOGY, V212, P474, DOI 10.1006/viro.1995.1505; LOVERING R, 1993, P NATL ACAD SCI USA, V90, P2112, DOI 10.1073/pnas.90.6.2112; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; Sambrook J, MOL CLONING LAB MANU; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; SENKEVICH TG, 1994, VIROLOGY, V198, P118, DOI 10.1006/viro.1994.1014; STOW ND, 1993, VIROLOGY, V196, P413, DOI 10.1006/viro.1993.1496; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VIEIRA J, 1987, METHOD ENZYMOL, V153, P3; WIRTH UV, 1992, J VIROL, V66, P2763, DOI 10.1128/JVI.66.5.2763-2772.1992	19	11	11	0	2	ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495	0042-6822			VIROLOGY	Virology	MAY 1	1996	219	1					301	303		10.1006/viro.1996.0251			3	Virology	Virology	UJ532	WOS:A1996UJ53200036	8623545				2021-04-07	
J	Schmid, R; Dring, MJ				Schmid, R; Dring, MJ			Influence of carbon supply on the circadian rhythmicity of photosynthesis and its stimulation by blue light in Ectocarpus siliculosus: Clues to the mechanism of inorganic carbon acquisition in lower brown algae	PLANT CELL AND ENVIRONMENT			English	Article						Ectocarpus; Phaeophyta; blue light; carbon acquisition; carbonic anhydrase (inhibitors); circadian rhythm; photosynthesis	ASCOPHYLLUM-NODOSUM PHAEOPHYCEAE; CRASSULACEAN ACID METABOLISM; MARINE MACROALGAE; PHOSPHOENOLPYRUVATE CARBOXYKINASE; FAST RESPONSES; SATURATED PHOTOSYNTHESIS; BUFFER SYSTEM; CARBOXYLASE; ASSIMILATION; ANHYDRASE	Stimulation of light-saturated rates of photosynthesis in Ectocarpus siliculosus (Dillwyn) Lyngb. by blue light was eliminated by increasing dissolved inorganic carbon (DIC) or by lowering pH in natural seawater. The amplitude of the circadian rhythm of photosynthesis was also diminished under these conditions, and the pH compensation points in a closed system were higher in the presence of blue light and during the circadian day. These observations suggest that blue light and the circadian clock regulate the activity of a carbon acquisition system in these plants. The inhibitor of external carbonic anhydrase, acetazolamide, reduced overall rates of photosynthesis by only about 30%, but ethoxyzolamide suppressed the circadian rhythm of photosynthesis almost completely and markedly reduced the duration of responses to blue light pulses. Similar patterns were obtained when photosynthesis was measured in strongly limiting DIC concentrations (0-0.5 mol m(-3)). Since blue light stimulated photosynthesis under these conditions of strong carbon limitation, we suggest that blue light activates the release of CO2 from an internal CO2 store. We propose a metabolic pathway with similarities to that of CAM plants. Non-photosynthetic fixation leads to the accumulation of a storage metabolite. The circadian clock and blue light control the mobilization of CO2 at the site of decarboxylation of this metabolite. In the presence of continuous blue light the pathway is proposed to cycle and act as a pump for CO2 into the chloroplasts. This hypothesis helps to explain a number of previously reported peculiarities of brown algal photosynthesis.	QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND			Dring, Matthew/B-4941-2014	Dring, Matthew/0000-0001-9043-5670			AKAZAWA H, 1972, PLANT CELL PHYSL, V13, P999; AXELSSON L, 1988, MAR BIOL, V97, P295, DOI 10.1007/BF00391315; AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P771, DOI 10.1111/j.1365-3040.1989.tb01638.x; AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P765, DOI 10.1111/j.1365-3040.1989.tb01637.x; Axelsson L., 1991, SEAWEED CELLULAR BIO, P185; Beer Sven, 1994, V10, P179; Bowes G., 1987, Plant life in aquatic and amphibious habitats., P79; BRULFERT J, 1986, BIOCHEM BIOPH RES CO, V136, P151, DOI 10.1016/0006-291X(86)90889-2; CARTER PJ, 1991, EMBO J, V10, P2063, DOI 10.1002/j.1460-2075.1991.tb07737.x; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; GIORDANO M, 1989, OECOLOGIA, V81, P534, DOI 10.1007/BF00378965; GRIFFITHS H, 1988, ADV BOT RES, V15, P43; HAGLUND K, 1992, PLANTA, V188, P1, DOI 10.1007/BF00198932; HUBER SC, 1975, CAN J BOT, V53, P1925, DOI 10.1139/b75-216; JOHNSTON AM, 1989, J PHYCOL, V25, P568, DOI 10.1111/j.1529-8817.1989.tb00263.x; JOHNSTON AM, 1991, CAN J BOT, V69, P1123, DOI 10.1139/b91-144; JOHNSTON AM, 1987, PHYCOLOGIA, V26, P159, DOI 10.2216/i0031-8884-26-2-159.1; JOHNSTON AM, 1987, J PHYCOL, V22, P78; JOSHI GV, 1974, PHOTOSYNTHETICA, V8, P51; KAREKAR MD, 1973, BOT MAR, V16, P216, DOI 10.1515/botm.1973.16.4.216; KERBY NW, 1985, ADV BOT RES, V11, P71, DOI 10.1016/S0065-2296(08)60169-X; KERBY NW, 1983, J PHYCOL, V19, P1, DOI 10.1111/j.0022-3646.1983.00001.x; KERBY NW, 1983, J PHYCOL, V19, P421, DOI 10.1111/j.0022-3646.1983.00421.x; Kremer B, 1981, OCEANOGR MAR BIOL AN, V19, P41; Kremer B. P., 1981, BIOL SEAWEEDS, P493; KREMER BP, 1980, PLANTA, V150, P189, DOI 10.1007/BF00582365; KREMER BP, 1977, PLANTA, V133, P191, DOI 10.1007/BF00391918; Leegood RC, 1990, PLANT PHYSL BIOCH MO, P274; LUCAS WJ, 1983, ANNU REV PLANT PHYS, V34, P71, DOI 10.1146/annurev.pp.34.060183.000443; LUNING K, 1985, MAR BIOL, V87, P119, DOI 10.1007/BF00539419; LUTTGE U, 1987, NEW PHYTOL, V106, P593, DOI 10.1111/j.1469-8137.1987.tb00163.x; MABERLY SC, 1990, J PHYCOL, V26, P439, DOI 10.1111/j.0022-3646.1990.00439.x; MADSEN TV, 1991, AQUAT BOT, V41, P5, DOI 10.1016/0304-3770(91)90037-6; MORONEY JV, 1985, PLANT PHYSIOL, V79, P177, DOI 10.1104/pp.79.1.177; NIMMO GA, 1987, PLANTA, V170, P408, DOI 10.1007/BF00395034; NIMMO GA, 1984, FEBS LETT, V178, P199, DOI 10.1016/0014-5793(84)80600-6; RAVEN JA, 1985, PLANT CELL ENVIRON, V8, P417, DOI 10.1111/j.1365-3040.1985.tb01677.x; SANCHO A, 1989, PLANT PHYSIOL BIOCH, V27, P537; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; Schmid R, 1996, PLANT CELL ENVIRON, V19, P383, DOI 10.1111/j.1365-3040.1996.tb00330.x; SCHMID R, 1996, IN PRESS SCI MARINA; Smith JAC, 1992, PLANT ORGANELLES COM, P141; SURIF MB, 1989, OECOLOGIA, V78, P97, DOI 10.1007/BF00377203; WEIDNER M, 1982, Z PFLANZENPHYSIOL, V108, P353, DOI 10.1016/S0044-328X(82)80181-5; WILKINS MB, 1992, NEW PHYTOL, V121, P347, DOI 10.1111/j.1469-8137.1992.tb02936.x	51	22	24	0	16	BLACKWELL SCIENCE LTD	OXFORD	OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL	0140-7791			PLANT CELL ENVIRON	Plant Cell Environ.	APR	1996	19	4					373	382		10.1111/j.1365-3040.1996.tb00329.x			10	Plant Sciences	Plant Sciences	UF158	WOS:A1996UF15800001					2021-04-07	
J	Schmid, R; Mills, JA; Dring, MJ				Schmid, R; Mills, JA; Dring, MJ			Influence of carbon supply on the stimulation of light-saturated photosynthesis by blue light in Laminaria saccharine: Implications for the mechanism of carbon acquisition in higher brown algae	PLANT CELL AND ENVIRONMENT			English	Article						Laminaria; blue light; carbon acquisition; carbonic anhydrase inhibitors; CO2 store; Phaeophyta photosynthesis	INORGANIC CARBON; ASCOPHYLLUM-NODOSUM; MARINE MACROALGAE; FAST RESPONSES; BUFFER SYSTEM; PHAEOPHYTA; AVAILABILITY; ADAPTATIONS; ECTOCARPUS; ANHYDRASE	In saturating irradiances of red light, photosynthesis of Laminaria saccharina (L.) Lamouroux was stimulated by low irradiances of continuous blue light only when the supply of dissolved inorganic carbon (DIC) was limiting. The degree of this stimulation was inversely proportional to the logarithm of the concentration of free CO2, whether this was adjusted by varying the total DIC or the pH at a given DIC concentration. The final pH reached in a closed system was higher in blue light than in red light. Both acetazolamide and ethoxyzolamide suppressed the responses to blue light almost completely, but reduced photosynthesis in red light by only 30%. Buffering the pH of the seawater also suppressed the stimulation of photosynthesis by blue light without affecting the photosynthetic rate in red light. The transient stimulation of O-2 evolution by a blue light pulse was not accompanied by a corresponding increase in CO2 consumption. These observations could be explained if, in analogy to the mechanism proposed for Ectocarpus (Schmid, Mills & Dring 1996, Plant Cell and Environment 19, 373-382, this issue, accompanying paper), photosynthesis was supported by a blue-light-activated release of CO2 from an internal store. We suggest that the store is located in the vacuoles of the cortical tissue of the blades. The main photosynthetic tissue, however, is in the overlying meristoderm, and blue-light-activated mobilization of the store could stimulate O-2 evolution only if periplasmic carbonic anhydrase was available to facilitate CO2 uptake from the cortex.	QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND			Dring, Matthew/B-4941-2014	Dring, Matthew/0000-0001-9043-5670			AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P771, DOI 10.1111/j.1365-3040.1989.tb01638.x; AXELSSON L, 1989, PLANT CELL ENVIRON, V12, P765, DOI 10.1111/j.1365-3040.1989.tb01637.x; COUDRET A, 1987, CR ACAD SCI III-VIE, V305, P177; COUDRET A, 1992, PHOTOSYNTHETICA, V26, P235; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; DROMGOOLE FI, 1988, BOT MAR, V31, P547, DOI 10.1515/botm.1988.31.6.547; DROMGOOLE FI, 1987, BOT MAR, V30, P331, DOI 10.1515/botm.1987.30.4.331; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; HAGLUND K, 1992, PLANTA, V188, P1, DOI 10.1007/BF00198932; JOHNSTON A M, 1990, British Phycological Journal, V25, P91; JOHNSTON AM, 1986, J PHYCOL, V22, P78, DOI 10.1111/j.1529-8817.1986.tb02518.x; Kremer B, 1981, OCEANOGR MAR BIOL AN, V19, P41; MABERLY SC, 1990, J PHYCOL, V26, P439, DOI 10.1111/j.0022-3646.1990.00439.x; MORONEY JV, 1985, PLANT PHYSIOL, V79, P177, DOI 10.1104/pp.79.1.177; NICHOLSON NL, 1972, AM J BOT, V59, P97, DOI 10.2307/2441235; SANCHO A, 1989, PLANT PHYSIOL BIOCH, V27, P537; Schmid R, 1996, PLANT CELL ENVIRON, V19, P373, DOI 10.1111/j.1365-3040.1996.tb00329.x; SCHMID R, 1994, J PHYCOL, V30, P612, DOI 10.1111/j.0022-3646.1994.00612.x; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; SCHMID R, 1996, IN PRESS SCI MARINA; SCHMITZ K, 1972, Z PFLANZENPHYSIOL, V67, P418, DOI 10.1016/S0044-328X(72)80042-4; SCHMITZ K, 1974, CYTOBIOLOGIE, V10, P66	25	27	30	0	7	BLACKWELL SCIENCE LTD	OXFORD	OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL	0140-7791			PLANT CELL ENVIRON	Plant Cell Environ.	APR	1996	19	4					383	391		10.1111/j.1365-3040.1996.tb00330.x			9	Plant Sciences	Plant Sciences	UF158	WOS:A1996UF15800002					2021-04-07	
J	Correa, JA; Gonzalez, P; Sanchez, P; Munoz, J; Orellana, MC				Correa, JA; Gonzalez, P; Sanchez, P; Munoz, J; Orellana, MC			Copper-algae interactions: Inheritance or adaptation?	ENVIRONMENTAL MONITORING AND ASSESSMENT			English	Article							ECTOCARPUS-SILICULOSUS; METAL POLLUTION; HONG-KONG; TOLERANCE; MARINE; EXCLUSION; MECHANISM; TOXICITY; CADMIUM; WATERS	This study evaluated the responses of wild, adult plants of Enteromorpha compressa, and their progeny, to various copper concentrations. Experiments were designed to test the hypotheses that: 1) individuals of E. compressa from Caleta Palito, a copper-enriched coastal locality, tolerate higher copper concentrations than those from a place with no history of copper pollution and 2) such copper tolerance is under genetic control and therefore, was an inherited character. Our results indicate that algae which inhabit a copper-enriched environment tolerate higher concentrations of copper than those from waters with low copper concentrations. On the other hand, our results suggest that generalizations regarding heritability of the tolerance to copper do not apply to the Chilean E. compressa, as no differences in growth or rhizoid production were found between the progeny from Caleta Palito and Caleta Zenteno. These findings are an indication that heritability and adaptation may represent alternative strategies used by different populations of the same algal species to tolerate copper.		Correa, JA (corresponding author), PONTIFICIA UNIV CATOLICA CHILE,FAC CIENCIAS BIOL,DEPT ECOL,CASILLA 114-D,SANTIAGO,CHILE.						CASTILLA JC, 1995, IN PRESS ENV MON ASS; CHAN J P, 1974, Marine Pollution Bulletin, V5, P171, DOI 10.1016/0025-326X(74)90131-3; CHUNG IK, 1986, MAR POLLUT BULL, V17, P213, DOI 10.1016/0025-326X(86)90603-X; CULLINANE JP, 1987, HYDROBIOLOGIA, V151, P285, DOI 10.1007/BF00046142; DANIEL GF, 1981, BOT MAR, V24, P229, DOI 10.1515/botm.1981.24.4.229; EVANS LN, 1986, ALGAL BIOFOULING; FOSTER PL, 1977, NATURE, V269, P322, DOI 10.1038/269322a0; GOODMAN C, 1976, INT BIODETERIOR, V12, P81; GUPTA AB, 1978, PHYSIOL PLANTARUM, V44, P215, DOI 10.1111/j.1399-3054.1978.tb08620.x; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; HO YB, 1990, HYDROBIOLOGIA, V203, P73, DOI 10.1007/BF00005615; HO YB, 1987, MAR POLLUT BULL, V18, P564, DOI 10.1016/0025-326X(87)90542-X; Lobban CS, 1985, PHYSL ECOLOGY SEAWEE; Maeda Shigeru, 1990, P109; Mclaclan J, 1982, SYNTHETIC DEGRADATIV, P71; PHILLIPS DJH, 1977, ENVIRON POLLUT, V13, P281, DOI 10.1016/0013-9327(77)90047-7; Provasoli L, 1963, P 4 INT SEAW S, P9; RAI LC, 1981, BIOL REV, V56, P99, DOI 10.1111/j.1469-185X.1981.tb00345.x; REED RH, 1983, J EXP MAR BIOL ECOL, V69, P85, DOI 10.1016/0022-0981(83)90173-9; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; SANDMANN G, 1985, PHYSIOL PLANTARUM, V65, P481, DOI 10.1111/j.1399-3054.1985.tb08677.x; SEELIGER U, 1977, MAR POLLUT BULL, V8, P16, DOI 10.1016/0025-326X(77)90398-8; SEELIGER U, 1982, ENVIRON POLLUT A, V29, P197, DOI 10.1016/0143-1471(82)90164-7; SEELIGER U, 1982, MAR POLLUT BULL, V13, P253, DOI 10.1016/0025-326X(82)90350-2; SHIOI Y, 1978, PHYSIOL PLANTARUM, V44, P434, DOI 10.1111/j.1399-3054.1978.tb01651.x; SHUBERT E, 1984, ALGAE ECOLOGICAL IND; SILVERBERG BA, 1976, J CELL BIOL, V69, P210, DOI 10.1083/jcb.69.1.210; Stenner R.D., 1975, MAR POLLUT B, V6, P89; TAKAMURA N, 1990, J APPL PHYCOL, V2, P293, DOI 10.1007/BF02180917; TALBOT V, 1982, AUST J MAR FRESH RES, V33, P779, DOI 10.1071/MF9820779; VERMEER K, 1991, B ENVIRON CONTAM TOX, V46, P242, DOI 10.1007/BF01691944; Wallner M., 1986, ARQ CIEN MAR, V25, P41	32	42	44	1	3	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0167-6369			ENVIRON MONIT ASSESS	Environ. Monit. Assess.	MAR	1996	40	1					41	54		10.1007/BF00395166			14	Environmental Sciences	Environmental Sciences & Ecology	TZ520	WOS:A1996TZ52000003	24198070				2021-04-07	
J	Katsaros, C; Reiss, HD; Schnepf, E				Katsaros, C; Reiss, HD; Schnepf, E			Freeze-fracture studies in brown algae: Putative cellulose-synthesizing complexes on the plasma membrane	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						cell wall; cellulose; freeze-fracture; particle complexes; Phaeophyta; plasma membrane	SUPRAMOLECULAR ORGANIZATION; ACETOBACTER-XYLINUM; PARTICLE ROSETTES; VISUALIZATION; BIOSYNTHESIS; CELL	Numerous single particles and particle complexes were observed on the protoplasmic fracture-face of the plasma membrane of the brown algae Ectocarpus siliculosus, Hincksia mitchelliae, Tilopteris mertensii, Sphacelaria rigidula, S. radicans and S. nana examined by freeze-fracture. The single particles do not show any particular distribution pattern, and their diameter varies from 7 nm to 10 nm. Two types of particle complex can be found: linear complexes, consisting of a single row of densely packed particles, and pentads of particles forming a rectangular configuration, with four particles in the corners and one in the centre. The number of particles in the linear complexes is between 6 and about 90. Their diameter is about 7.5 nm, which is smaller than that of most single particles. Although the linear complexes are not strictly oriented, two main axes can be distinguished which form an angle of about 45-90 degrees. This orientation appears to be parallel to that of the cellulose microfibrils. The linear complexes are frequently connected with the end of microfibril imprints (terminal complexes). The size of the particles of the pentads is similar to that of the single particles, and the side of the square is about 14 nm. The distribution and density of the pentads are variable. The pentads may be rare (e.g. in E. siliculosus), or there can be as many as 300 mu m(-2) (e.g. in T. mertensii). No such particle complexes were found on the extraplasmic fracture-face of the plasma membrane. The possible role of the above particle complexes is discussed.	UNIV HEIDELBERG, W-6900 HEIDELBERG, GERMANY	Katsaros, C (corresponding author), UNIV ATHENS, DEPT BIOL, INST GEN BOT, GR-15784 ATHENS, GREECE.						BAILEY A, 1969, PHYCOLOGIA, V8, P57; BROWN RM, 1976, P NATL ACAD SCI USA, V73, P143, DOI 10.1073/pnas.73.1.143; BROWN RM, 1976, P NATL ACAD SCI USA, V73, P4565, DOI 10.1073/pnas.73.12.4565; DAWES CJ, 1961, AM J BOT, V48, P925, DOI 10.2307/2439535; DELMER DP, 1987, ANNU REV PLANT PHYS, V38, P259; Delmer DP, 1991, CYTOSKELETAL BASIS P, P100; Emons AMC, 1991, BIOSYNTHESIS BIODEGR, P71; Giddings T.H., 1991, CYTOSKELETAL BASIS P, P85; GILKEY JC, 1989, PLANTA, V178, P425, DOI 10.1007/BF00963811; Herth W, 1985, BOTANICAL MICROSCOPY, P285; Hiagler C. H., 1985, CELLULOSE CHEM ITS A, P30; HOTCHKISS AT, 1989, ACS SYM SER, V399, P232; ITOH T, 1990, J CELL SCI, V95, P309; KLINK R, 1990, BOT ACTA, V103, P24, DOI 10.1111/j.1438-8677.1990.tb00121.x; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; MIZUTA S, 1992, PROTOPLASMA, V166, P187, DOI 10.1007/BF01322781; MIZUTA S, 1989, CELLULOSE WOOD CHEM, P659; MUELLER SC, 1980, J CELL BIOL, V84, P315, DOI 10.1083/jcb.84.2.315; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; OKUDA K, 1992, PROTOPLASMA, V168, P51, DOI 10.1007/BF01332650; Okuda K., 1993, Japanese Journal of Phycology, V41, P151; OKUDA K, 1994, PROTOPLASMA, V180, P49, DOI 10.1007/BF01379223; PENG HB, 1976, PLANTA, V133, P57, DOI 10.1007/BF00386007; PRESTON RD, 1974, PHYSICAL BIOL PLANT; Provasoli L., 1968, CULTURES COLLECTIONS, P63; Quader H., 1991, BIOSYNTHESIS BIODEGR, P51; Quatrano RS, 1982, CELLULOSE OTHER NATU, P45; REISS HD, 1984, PLANTA, V160, P428, DOI 10.1007/BF00429759; ROBINSON DG, 1974, PLANTA, V104, P234; RUDOLPH U, 1989, PROTOPLASMA, V148, P57, DOI 10.1007/BF02079323; Silva P.C., 1987, SMITHSON CONTRIB MAR, V27, P1, DOI DOI 10.5479/SI.1943667X.27.1; TSEKOS I, 1993, ANN BOT-LONDON, V72, P213, DOI 10.1006/anbo.1993.1101; TSEKOS I, 1994, J PHYCOL, V30, P300, DOI 10.1111/j.0022-3646.1994.00300.x; TSEKOS I, 1993, ACTA BOT NEERL, V42, P119; TSEKOS I, 1992, PROTOPLASMA, V169, P57, DOI 10.1007/BF01343370; TSEKOS I, 1988, J ULTRA MOL STRUCT R, V99, P156, DOI 10.1016/0889-1605(88)90051-1; VANREINE WFP, 1982, LEIDEN BOTANICAL SER, V6; WADA M, 1981, PLANTA, V151, P462, DOI 10.1007/BF00386540; WITTE O, 1991, THESIS U HEIDELBERG; ZAAR K, 1979, J CELL BIOL, V80, P773, DOI 10.1083/jcb.80.3.773; ZAAR K, 1977, CYTOBIOLOGIE, V16, P1	41	12	13	0	1	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0967-0262	1469-4433		EUR J PHYCOL	Eur. J. Phycol.	FEB	1996	31	1					41	48		10.1080/09670269600651171a			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	UA491	WOS:A1996UA49100005					2021-04-07	
J	Sengco, MR; Brautigam, M; Kapp, M; Muller, DG				Sengco, MR; Brautigam, M; Kapp, M; Muller, DG			Detection of virus DNA in Ectocarpus siliculosus and E-fasciculatus (Phaeophyceae) from various geographic areas	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						DNA virus; Ectocarpus; E-fasciculatus; E-siliculosus; epidemiology; geographic survey; pandemy; PCR	MARINE; ALGAE; INFECTION; GENOME; HOST	The filamentous marine brown algae Ectocarpus siliculosus and E. fasciculatus are frequently infected by DNA viruses. We used polymerase chain reaction amplification (PCR) of a virus-specific nucleotide sequence to detect the presence of viral DNA in extracts of Ectocarpus plants, and applied this technique to a collection of 97 unialgal Ectocarpus cultures from coasts of all oceans and continents. We found that 42 isolates contained viral DNA. Among these were four sporophytes, which produced gametophytes free of virus DNA. This observation supports previous studies showing that the viral genome segregates like a Mendelian trait during meiosis. The pandemy and epidemiology of the host-virus relationship in Ectocarpus is discussed.		Sengco, MR (corresponding author), UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY.						BRAUTIGAM M, 1995, J PHYCOL, V31, P823, DOI 10.1111/j.0022-3646.1995.00823.x; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; GOFF LJ, 1993, J PHYCOL, V29, P381, DOI 10.1111/j.0022-3646.1993.00381.x; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; JOHN ME, 1992, NUCLEIC ACIDS RES, V20, P2381, DOI 10.1093/nar/20.9.2381; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MULLER DG, 1976, J PHYCOL, V12, P252, DOI 10.1111/j.0022-3646.1976.00252.x; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1994, EUR J PHYCOL, V29, P219, DOI 10.1080/09670269400650671; MULLER DG, 1996, IN PRESS P INT SEAWE, V15; MULLER DG, 1996, IN PRESS PHYCOLOGIA, V35; Muller Dieter G., 1995, Phycological Research, V43, P175, DOI 10.1111/j.1440-1835.1995.tb00022.x; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; STACHE B, 1990, NATO ASI SERIES G, V22, P173; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; White T.J., 1990, PCR PROTOCOLS GUIDE, P315	23	25	25	0	6	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	FEB	1996	31	1					73	78		10.1080/09670269600651221			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	UA491	WOS:A1996UA49100010		Bronze			2021-04-07	
J	Tan, IH; Druehl, LD				Tan, IH; Druehl, LD			A ribosomal DNA phylogeny supports the close evolutionary relationships among the Sporochnales, Desmarestiales, and Laminariales (Phaeophyceae)	JOURNAL OF PHYCOLOGY			English	Article						18S rDNA; Chordaceae; Desmarestiales; Laminariales; Phaeophyceae; Phyllariaceae; phylogeny; Sporochnales; taxonomy	NEIGHBOR-JOINING METHOD; NUCLEOTIDE-SEQUENCES; CONFIDENCE-LIMITS; CHORDA-TOMENTOSA; ALGAE; BOOTSTRAP	We report six complete 18S ribosomal DNA (rDNA) sequences representing five brown algal orders: Sporochnus comosus C. A. Agardh (Sporochnales), Chorda tomentosa Lyngbye (Chordaceae, Laminariales), Saccorhiza polyschides (Lightfoot) Batters (Phyllariaceae, Laminariales), Desmarestia ligulata (Lightfoot) Lamouroux (Desmarestiales), Ectocarpus siliculosus (Dillwyn) Lyngbye (Ectocarpales), and Scytosiphon lomentaria (Lyngbye) J. G. Agardh (Scytosiphonales). These sequences were compared with published laminarialean (Alaria marginata Postel et Ruprecht [Alariaceae] and Macrocystis integrifolia Bory [Lessoniaceae]) and fucalean (Fucus gardneri Silva) rDNA sequences for phylogeny inference using both the distance-matrix and parsimony methods. The inferred 18S phylogenies clustered Sporochnus, Desmarestia, Chorda, Saccorhiza, Alaria, and Macrocystis in an assemblage. This Sporochnales-Desmarestiales-Laminariales (S-D-L) complex was consistently separated from the Ectocarpales, Scytosiphonales, and Fucales by bootstrap analyses. The inferred phylogenies are consistent with several possible evolutionary processes lending to this S-D-L complex. Members in this assemblage lack eyespots in their sperm, and their sperm have the atypical brown algal flagellation: shelter anterior and longer posterior flagella. In addition, they are oogamous with a heteromorphic alternation of generations between a microscopic gametophyte and a macroscopic sporophyte. Members of the S-D-L complex can be separated into different phylogenetic lines based on the presence/absence of eyespots in their meiospores. Our findings support the contention that the Sporochnales, Desmarestiales, and Laminariales are closely related. In addition, our rDNA tree suggests that the Laminariales is paraphyletic.	BAMFIELD MARINE STN,BAMFIELD,BC V0R 1B0,CANADA	Tan, IH (corresponding author), SIMON FRASER UNIV,DEPT BIOL SCI,BURNABY,BC V5A 1S6,CANADA.						ARIZTIA EV, 1991, J PHYCOL, V27, P428, DOI 10.1111/j.0022-3646.1991.00428.x; BHATTACHARYA D, 1992, EVOLUTION, V46, P1801, DOI 10.1111/j.1558-5646.1992.tb01170.x; BILOFSKY HS, 1988, NUCLEIC ACIDS RES, V16, P1861, DOI 10.1093/nar/16.5.1861; CABOT EL, 1989, COMPUT APPL BIOSCI, V5, P233; CLAYTON MN, 1984, PROGR PHYCOLOGICAL R, V3, P11; DRUEHL LD, 1992, PROG PHYCOL RES, V8, P47; EMERSON CJ, 1982, CAN J BOT, V60, P2164, DOI 10.1139/b82-266; FAIN SR, 1986, THESIS S FRASER U BU; FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x; FRITSCH FE, 1945, STRUCTURE REPRODUCTI, V2; HENRY EC, 1982, J PHYCOL, V18, P570; HILLIS DM, 1992, J HERED, V83, P189, DOI 10.1093/oxfordjournals.jhered.a111190; Kawai H., 1992, KOREAN J PHYCOLOGY, V7, P33; KIM J, 1993, EVOLUTION, V47, P471, DOI [10.2307/2410065, 10.1111/j.1558-5646.1993.tb02107.x]; KIMURA M, 1980, J MOL EVOL, V16, P111, DOI 10.1007/BF01731581; Kumar S, 1993, MEGA MOL EVOLUTIONAR; MAIER I, 1984, NATURWISSENSCHAFTEN, V71, P48, DOI 10.1007/BF00365988; MAIER I, 1984, BRIT PHYCOL J, V19, P95, DOI 10.1080/00071618400650101; MAIER I, 1984, THESIS U KONSTANZ GE; MAYES C, 1992, J PHYCOL, V28, P712, DOI 10.1111/j.0022-3646.1992.00712.x; MOTOMURA T, 1985, Japanese Journal of Phycology, V33, P21; MULLER DG, 1985, PHYCOLOGIA, V24, P475, DOI 10.2216/i0031-8884-24-4-475.1; MULLER DG, 1988, BIOL CHEM H-S, V369, P655, DOI 10.1515/bchm3.1988.369.2.655; MULLER DG, 1982, NATURWISSENSCHAFTEN, V69, P290, DOI 10.1007/BF00396442; MULLER DG, 1985, PHYCOLOGIA, V24, P467, DOI 10.2216/i0031-8884-24-4-467.1; Nakamura Y., 1972, CONTRIBUTIONS SYSTEM, P147; PARKE M, 1976, J MAR BIOL ASSOC UK, V56, P527, DOI 10.1017/S002531540002066X; RUSSELL G, 1975, J MAR BIOL ASSOC UK, V55, P763, DOI 10.1017/S0025315400017690; SAIKI RK, 1988, SCIENCE, V239, P487, DOI 10.1126/science.2448875; SAITOU N, 1989, MOL BIOL EVOL, V6, P514; SAITOU N, 1987, MOL BIOL EVOL, V4, P406, DOI 10.1093/oxfordjournals.molbev.a040454; SANDERSON MJ, 1989, CLADISTICS, V5, P113, DOI 10.1111/j.1096-0031.1989.tb00559.x; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; SAUNDERS GW, 1992, J PHYCOL, V28, P544, DOI 10.1111/j.0022-3646.1992.00544.x; SAUNDERS GW, 1995, PHYCOLOGIA, V34, P383, DOI 10.2216/i0031-8884-34-5-383.1; SCAGEL RG, 1966, OCEANOG MAR BIOL ANN, V4, P123; SOUTH G. R., 1967, BRIT PHYCOL BULL, V3, P379; SWOFFORD DL, 1993, PAUP PHYLOGENETIC AN; Tan Ian H., 1993, Hydrobiologia, V260-261, P699, DOI 10.1007/BF00049090; TAN IH, 1994, J PHYCOL, V30, P721, DOI 10.1111/j.0022-3646.1994.00721.x; WYNNE MJ, 1976, PHYCOLOGIA, V15, P425	41	38	38	0	12	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	FEB	1996	32	1					112	118		10.1111/j.0022-3646.1996.00112.x			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	TX431	WOS:A1996TX43100014					2021-04-07	
J	Muller, DG; Brautigam, M; Knippers, R				Mueller, D. G.; Brautigam, M.; Knippers, R.			Virus infection and persistence of foreign DNA in the marine brown alga Feldmannia simplex (Ectocarpales, Phaeophyceae)	PHYCOLOGIA			English	Article								Endogenous viruses are found in the two filamentous marine brown algae Ectocarpus siliculosus (Dillwyn) Lyngbye and Feldmannia simplex (Crouan) Hamel. We have performed experiments showing that the Ectocarpus siliculosus virus (EsV-1) is able to infect zoospores of Feldmannia. EsV-1 did not multiply in Feldmannia, but its presence caused malformations of the host. The symptoms soon disappeared and the plants recovered to normal habit and fertility. After two years of culture we subjected DNA of EsV-1-infected Feldmannia to PCR amplification with oligomer primers specific for a gene that codes for a coat protein of the Ectocarpus Virus. Our results indicate that Ectocarpus virus DNA Can persist in Feldmannia, and that phenotypically normal Feldmannia simpler can harbour latent Ectocarpus virus DNA.	[Mueller, D. G.; Brautigam, M.; Knippers, R.] Univ Konstanz, Fak Biol, D-78434 Constance, Germany	Muller, DG (corresponding author), Univ Konstanz, Fak Biol, D-78434 Constance, Germany.				Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [Kn 126/11-3, Mu 196/18-2]	This work was supported by Deutsche Forschungsgemeinschaft (Kn 126/11-3 and Mu 196/18-2).	BRAUTIGAM M, 1995, J PHYCOLOGY IN PRESS; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; GOEE LJ, 1993, J PHYCOL, V29, P381; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1996, HYDROBIOLOG IN PRESS; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x	13	9	9	0	1	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA	0031-8884			PHYCOLOGIA	Phycologia	JAN	1996	35	1					61	63		10.2216/i0031-8884-35-1-61.1			3	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	V50JF	WOS:000203403900007					2021-04-07	
J	Muller, DG; Wolf, S; Parodi, ER				Muller, DG; Wolf, S; Parodi, ER			A virus infection in Myriotrichia clavaeformis (Dictyosiphonales, Phaeophyceae) from Argentina	PROTOPLASMA			English	Article						Dictyosiphonales; infection; Myriotrichia clavaeformis; Phaeophyceae; virus	ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; DNA VIRUS; MARINE; CULTURE; ALGAE; PARTICLES	Virus-infected plants of Myriotrichia clavaeformis from the coast of Argentina are sterile and produce virus particles in later al vesicles homologous to plurilocular sporangia of normal plants. Virions are released into the surrounding sea water and can infect swimming spores of healthy Myriotrichia plants. Virus particles are hexagonal in cross section with a diameter of 170-180 nm and have an envelope with an electron dense core. Similar viruses are known for several genera in the order Ectocarpales. This is the first record of an infectious agent in the order Dictyosiphonales, which contains thalli with more complex tissues and life histories.	UNIV NACL SUR,DEPT BIOL & BIOQUIM,BAHIA BLANCA,ARGENTINA; INST ARGENTINO OCEANOG,BAHIA BLANCA,ARGENTINA	Muller, DG (corresponding author), UNIV KONSTANZ,FAK BIOL,POSTFACH 5560,D-78434 CONSTANCE,GERMANY.						Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; Fletcher RL, 1987, SEAWEEDS BRIT ISLE 1, VIII; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P653, DOI 10.1111/j.0022-3646.1994.00653.x; Joly A., 1967, GENEROS ALGAS MARINH; LACLAIRE JW, 1977, PROTOPLASMA, V93, P127, DOI 10.1007/BF01276287; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; MULLER DG, 1996, IN PRESS P INT SEAWS, V15; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; PETERS AF, 1988, BRIT PHYCOL J, V23, P299, DOI 10.1080/00071618800650331; SAUVAGEAU C, 1896, J BOTANIQUE, V10, P140; SAUVAGEAU C, 1897, J BOT, V11, P66; Sengco MR, 1996, EUR J PHYCOL, V31, P73, DOI 10.1080/09670269600651221; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; TOTH R, 1972, J PHYCOL, V8, P126, DOI 10.1111/j.1529-8817.1972.tb04011.x; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; Womersley H.B.S., 1987, MARINE BENTHIC FLORA; Wynne M. J., 1982, SYNOPSIS CLASSIFICAT, V1, P115	24	7	7	0	0	SPRINGER-VERLAG WIEN	VIENNA	SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA	0033-183X			PROTOPLASMA	Protoplasma		1996	193	1-4					58	62		10.1007/BF01276634			5	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	VL135	WOS:A1996VL13500006					2021-04-07	
J	Kawai, H; Nakamura, S; Mimuro, M; Furuya, M; Watanabe, M				Kawai, H; Nakamura, S; Mimuro, M; Furuya, M; Watanabe, M			Microspectrofluorometry of the autofluorescent flagellum in phototactic brown algal zoids	PROTOPLASMA			English	Article						Chorda filum; flagellar fluorescence; flavin; microspectrofluorometry; phototaxis; Scytosiphon lomentaria	FLAVIN-LIKE FLUORESCENCE; EUGLENA-GRACILIS; ECTOCARPUS-SILICULOSUS; PARAFLAGELLAR BODY; PTERIN-LIKE; PHOTORECEPTOR; CHLAMYDOMONAS; MICROSCOPY; RESPONSES; PIGMENTS	Posterior flagella of zoids of Scytosiphon lomentaria and Chorda filum (Phaeophyceae, Chromophyta) were isolated and subjected to microspectrofluorometry using a high sensitivity microspectrofluorometer in order to characterize the flagellar autofluorescent substances. Vigorous agitation in a hypertonic medium yielded preparations of largely intact flagella retaining detectable green flagellar autofluorescence. Under 380-425 nm excitation light, maximum emission of flagellar autofluorescence was observed at 495 nm, whereas under 400-440 nm excitation light fluorescence shifted to around 510 nm. Comparison of these spectra with the fluorescence spectra of 4',5'-cyclic FMN isolated from fertile thalli of S. lomentaria ia, and of 6-carboxypterin suggested that two (or more) different fluorescent substances (presumably a flavin and a pterin) are present in the flagella.	TOYAMA UNIV,FAC SCI,DEPT ENVIRONM BIOL & CHEM,TOYAMA 930,JAPAN; NATL INST BASIC BIOL,OKAZAKI,AICHI 444,JAPAN	Kawai, H (corresponding author), KOBE UNIV,RES CTR INLAND SEAS,KOBE 657,JAPAN.						AUCLAIR W, 1966, SCIENCE, V154, P913, DOI 10.1126/science.154.3751.913; BENEDETTI PA, 1975, PLANT SCI LETT, V4, P47, DOI 10.1016/0304-4211(75)90074-7; BENEDETTI PA, 1977, PHOTOCHEM PHOTOBIOL, V26, P315, DOI 10.1111/j.1751-1097.1977.tb07492.x; BRODHUN B, 1990, PHOTOCHEM PHOTOBIOL, V51, P865; Dodge JD, 1973, FINE STRUCTURE ALGAL; FOSTER KW, 1984, NATURE, V311, P756, DOI 10.1038/311756a0; GALLAND P, 1990, PHOTOCHEM PHOTOBIOL, V51, P675, DOI 10.1111/php.1990.51.6.675; GALLAND P, 1988, J PHOTOCH PHOTOBIO B, V1, P277, DOI 10.1016/1011-1344(88)85016-4; GALLAND P, 1988, PHOTOCHEM PHOTOBIOL, V48, P811, DOI 10.1111/j.1751-1097.1988.tb02896.x; GHETTI F, 1985, PHOTOCHEM PHOTOBIOL, V42, P29, DOI 10.1111/j.1751-1097.1985.tb03543.x; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; KAWAI H, 1991, PROTOPLASMA, V161, P17, DOI 10.1007/BF01328893; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KAWAI H, 1988, J PHYCOL, V24, P114; KAWAI H, 1992, BOT MAG TOKYO, V105, P171, DOI 10.1007/BF02489413; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KREIMER G, 1994, INT REV CYTOL, V148, P229, DOI 10.1016/S0074-7696(08)62409-2; MIMURO M, 1988, BIOCHIM BIOPHYS ACTA, V933, P478, DOI 10.1016/0005-2728(88)90083-7; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; SCHMIDT W, 1990, PLANTA, V182, P375, DOI 10.1007/BF02411388; SINESHCHEKOV VA, 1994, J PHOTOCH PHOTOBIO B, V23, P225, DOI 10.1016/1011-1344(94)07002-4; Watanabe M, 1995, CRC HDB ORGANIC PHOT, P1260; WITMAN GB, 1978, J CELL BIOL, V76, P729, DOI 10.1083/jcb.76.3.729; WITMAN GB, 1972, J CELL BIOL, V54, P507, DOI 10.1083/jcb.54.3.507; YAMANO K, 1993, 35 S CHEM NAT PROD K, P646	26	9	9	0	0	SPRINGER-VERLAG WIEN	VIENNA	SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA	0033-183X			PROTOPLASMA	Protoplasma		1996	191	3-4					172	177		10.1007/BF01281815			6	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	UK030	WOS:A1996UK03000007					2021-04-07	
J	Satoh, M; Hori, T; Tsujimoto, K; Sasa, T				Satoh, M; Hori, T; Tsujimoto, K; Sasa, T			Isolation of eyespots of green algae and analyses of pigments	BOTANICA MARINA			English	Article							PERFORMANCE LIQUID-CHROMATOGRAPHY; LASER SCANNING MICROSCOPY; CHLAMYDOMONAS-REINHARDTII; PHOTORECEPTIVE ORGANELLE; ECTOCARPUS-SILICULOSUS; RHODOPSIN; PHOTOTAXIS; SIPHONAXANTHIN; RESPONSES	Eyespot granules were isolated from cells of Pyramimonas parkeae Norris et Pearson (Prasinophyceae) and their morphology and pigment composition were analyzed. Isolated eyespot granules were fixed with osmium tetroxide and examined by scanning and transmission electron microscopy. The isolated eyespot granules were 0.15-0.20 mu m in diameter, and were associated with several kinds of structures, e.g. membranes. Eyespot pigments were analyzed by high performance liquid chromatography (HPLC). The major components were alpha-, beta-, and gamma-carotene, other pigments were virtually absent. The ratio of alpha- and beta-carotenes was 1:6. Eyespots of Mesostigma viride Lauterborn (Prasinophyceae) and female gametes of Bryopsis maxima Okamura (Ulvophyceae) were also isolated, and their pigments were investigated. The major pigment of isolated eyespots of M. viride was carotene, that of B. maxima was alpha-carotene.	UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN; JAPAN ADV INST SCI & TECHNOL,SCH MAT SCI,NOMI,ISHIKAWA 92312,JAPAN; NATL INST ENVIRONM STUDIES,TSUKUBA,IBARAKI 305,JAPAN							BARTLETT CJ, 1972, PLANT PHYSIOL, V49, P881, DOI 10.1104/pp.49.6.881; Foster K.W., 1989, Plant Biology (New York), V7, P215; FOSTER KW, 1984, NATURE, V311, P756, DOI 10.1038/311756a0; FOSTER KW, 1980, MICROBIOL REV, V44, P572, DOI 10.1128/MMBR.44.4.572-630.1980; FOSTER KW, 1988, P NATL ACAD SCI USA, V85, P6379, DOI 10.1073/pnas.85.17.6379; Gross J, 1987, SERIES MONOGRAPHS FO, P87; HEGEMANN P, 1988, PHOTOCHEM PHOTOBIOL, V48, P123; HEGEMANN P, 1991, BIOPHYS J, V60, P1477, DOI 10.1016/S0006-3495(91)82183-X; HORI T, 1988, Japanese Journal of Phycology, V36, P113; HORI T, 1979, Japanese Journal of Phycology, V27, P183; Karrer P, 1943, HELV CHIM ACTA, V26, P2121, DOI 10.1002/hlca.19430260707; KATO S, 1981, JAP J PHYCOL, V30, P63; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KOHATA K, 1988, J PHYCOL, V24, P58; KOYAMA Y, 1988, J CHROMATOGR, V439, P417, DOI 10.1016/S0021-9673(01)83855-3; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KREIMER G, 1991, FEBS LETT, V293, P49, DOI 10.1016/0014-5793(91)81150-7; KREIMER G, 1990, EUR J CELL BIOL, V53, P101; KREIMER G, 1992, PLANTA, V188, P513, DOI 10.1007/BF00197043; KREIMER G, 1991, EUR J CELL BIOL, V55, P318; MANTOURA RFC, 1983, ANAL CHIM ACTA, V151, P297, DOI 10.1016/S0003-2670(00)80092-6; Melkonian M., 1984, Progress phycol. Res., V3, P193; NULTSCH W, 1988, PHOTOCHEM PHOTOBIOL, V47, P837, DOI 10.1111/j.1751-1097.1988.tb01668.x; OKELLY CJ, 1982, BOT MAR, V25, P133, DOI 10.1515/botm.1982.25.3.133; PAGNI PGS, 1981, PHYCOLOGIA, V20, P431, DOI 10.2216/i0031-8884-20-4-431.1; Provasoli L., 1960, Special Publications Pymatuning Laboratory of Field Ecology, VNo. 2, P84; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; Rowan K. S., 1989, PHOTOSYNTHETIC PIGME, P112; Strain H.H., 1951, MANUAL PHYCOLOGY, P243; STRAIN HH, 1965, BIOL BULL, V129, P366, DOI 10.2307/1539852; TAKAHASHI T, 1991, BIOCHEM BIOPH RES CO, V178, P1273, DOI 10.1016/0006-291X(91)91031-7; YOKOHAMA Y, 1981, BOT MAR, V24, P637; YOKOHAMA Y, 1977, BOT MAR, V20, P433, DOI 10.1515/botm.1977.20.7.433; YOKOHAMA Y, 1981, JPN J PHYCOL, V30, P311	34	5	5	0	6	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055			BOT MAR	Bot. Marina	NOV	1995	38	6					467	474		10.1515/botm.1995.38.1-6.467			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	TN137	WOS:A1995TN13700002					2021-04-07	
J	Makarov, VN; Schoschina, EV; Luning, K				Makarov, VN; Schoschina, EV; Luning, K			Diurnal and circadian periodicity of mitosis and growth in marine macroalgae .1. Juvenile sporophytes of Laminariales (Phaeophyta)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						circadian rhythm; cell division; diurnal rhythm; Laminariales	ECTOCARPUS PHAEOPHYTA; FAST RESPONSES; CELL-DIVISION; BLUE-LIGHT; RED ALGA; PHOTOSYNTHESIS; RHYTHM; DNA	In juvenile sporophytes of Laminaria schinzii, Laminaria sinclairii and Plerygophora californica grown at 12 h light per day the majority of nuclear divisions took place during the dark interval. In P. californica it was ascertained that the rhythm in nuclear division persisted in continuous light for at least six cycles and thus was controlled by the circadian system. Under light-dark conditions, a night peak occurred for division and a morning peak for growth rate. Maxima of the free-running division rhythm preceded maxima of the free-running growth rhythm by about 6 h. Sporophytic cells of L. saccharina with a generation time of up to 3 days continued to grow between two cell divisions so that thallus expansion rhythmicity may reflect mainly the growth behaviour of non-dividing cells.	BIOL ANSTALT HELGOLAND, D-22607 HAMBURG, GERMANY	Makarov, VN (corresponding author), MURMANSK MARINE BIOL INST, MURMANSKAJA OBL, MURMANSK 184631, RUSSIA.						BUHNEMANN F, 1955, PLANTA, V46, P227; CHISHOLM SW, 1981, J EXP MAR BIOL ECOL, V51, P107, DOI 10.1016/0022-0981(81)90123-4; Edmunds L. N., 1984, CELL CYCLE CLOCKS; Edmunds LN, 1988, CELLULAR MOL BASES B; Evans GC, 1972, QUANTITATIVE ANAL PL; FRITSCH FE, 1959, STRUCTURE REPRODUCTI, V2; GOFF L. J., 1990, BIOL RED ALGAE, P43; GOFF LJ, 1984, DEV BIOL, V102, P173, DOI 10.1016/0012-1606(84)90183-0; HADER DP, 1988, P INDIAN AS-PLANT SC, V98, P227; HADER DP, 1988, J PHOTOCH PHOTOBIO B, V1, P385, DOI 10.1016/1011-1344(88)85001-2; HULL HM, 1982, STAIN TECHNOL, V57, P273, DOI 10.3109/10520298209066723; KAGEYAMA A, 1979, BOT MAR, V22, P199, DOI 10.1515/botm.1979.22.4.199; KAIN JM, 1987, PHYCOLOGIA, V26, P88, DOI 10.2216/i0031-8884-26-1-88.1; Kain JM., 1979, OCEANOGR MAR BIOL AN, V17, P101; LUNING K, 1975, MAR BIOL, V29, P195, DOI 10.1007/BF00391846; LUNING K, 1994, J PHYCOL, V30, P193, DOI 10.1111/j.0022-3646.1994.00193.x; LUNING K, 1992, J PHYCOL, V28, P794, DOI 10.1111/j.0022-3646.1992.00794.x; LUNING K, 1981, BR J PHYCOL, V16, P579; NEUSCHELERWIRTH H, 1970, Z PFLANZENPHYSIOL, V63, P352; NULTSCH W, 1984, MAR BIOL, V81, P217, DOI 10.1007/BF00393215; OOHUSA T, 1980, BOT MAR, V23, P1, DOI 10.1515/botm.1980.23.1.1; OSTGAARD K, 1982, MAR BIOL, V66, P261, DOI 10.1007/BF00397031; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Starr R.C., 1987, Journal of Phycology, V23, P1; Sweeney B.M., 1983, Progress phycol. Res., V2, P189; SWEENEY BM, 1982, PLANT PHYSIOL, V70, P272, DOI 10.1104/pp.70.1.272; SWEENEY BM, 1958, J PROTOZOOL, V5, P217, DOI 10.1111/j.1550-7408.1958.tb02555.x; SWEENEY BM, 1987, RHYTHMIC PHENOMENA P; VOGEL K, 1990, 4TH P EUR S LIF SCI, P541; WAALAND SD, 1972, PLANTA, V105, P196, DOI 10.1007/BF00385391	31	25	27	0	6	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0967-0262	1469-4433		EUR J PHYCOL	Eur. J. Phycol.	NOV	1995	30	4					261	266		10.1080/09670269500651031			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	TM987	WOS:A1995TM98700003					2021-04-07	
J	Maier, I				Maier, I			On the fine structure of flagellar hairs in the brown algae (Phaeophyceae)	PHYCOLOGIA			English	Article							DICTYOTALES	The fine structure of the tripartite tubular flagellar hairs in male gametes of the brown algae Ectocarpus siliculosus (Dillwyn) Lyngbye, Laminaria digitata (Hudson) Lamouroux, Fucus serratus Linnaeus, Hormosira banksii (Turner) Decaisne and Durvillaea potatorum (Labillardiere) Areschoug has been investigated by negative staining. In all these species three terminal filaments are present, with a long central filament and two thinner, shorter and equal filaments inserted laterally to the central one. The distribution of this feature is discussed in relation to the phylogenetic position of the Phaeophyceae.		Maier, I (corresponding author), UNIV KONSTANZ, FAK BIOL, POSTFACH 5560, D-78434 CONSTANCE, GERMANY.						BOUCK GB, 1969, J CELL BIOL, V40, P446, DOI 10.1083/jcb.40.2.446; BRADLEY DE, 1966, EXP CELL RES, V41, P162, DOI 10.1016/0014-4827(66)90556-8; CHEN LL, 1976, J BIOL CHEM, V251, P1828; JAHN TL, 1964, J PROTOZOOL, V11, P291, DOI 10.1111/j.1550-7408.1964.tb01756.x; Kawano L.S., 1984, P76; LEADBEATER BSC, 1989, SYST ASSOC SPEC VOL, V38, P145; LEEDALE GF, 1970, J CELL SCI, V6, P701; LEIPE DD, 1994, PHYCOLOGIA, V33, P369, DOI 10.2216/i0031-8884-33-5-369.1; LOISEAUX S, 1970, T AM MICROSC SOC, V89, P524, DOI 10.2307/3224562; MAIER I, 1982, PHYCOLOGIA, V21, P1, DOI 10.2216/i0031-8884-21-1-1.1; MAIER I, 1995, IN PRESS PHYCOLOGICA, V43; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; OKELLY CJ, 1989, SYST ASSOC SPEC VOL, V38, P255; PATTERSON DJ, 1989, SYST ASSOC SPEC VOL, V38, P357; PHILLIPS JA, 1990, PHYCOLOGIA, V29, P367, DOI 10.2216/i0031-8884-29-3-367.1; PHILLIPS JA, 1991, PHYCOLOGIA, V30, P205, DOI 10.2216/i0031-8884-30-2-205.1; VANDENHOEK C, 1993, ALGEN THIEME STUTTGA; WRIGLEY NG, 1968, J ULTRA MOL STRUCT R, V24, P454, DOI 10.1016/S0022-5320(68)80048-6	20	4	4	1	8	TAYLOR & FRANCIS LTD	ABINGDON	2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND	0031-8884	2330-2968		PHYCOLOGIA	Phycologia	NOV	1995	34	6					441	443		10.2216/i0031-8884-34-6-441.1			3	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	TK677	WOS:A1995TK67700001					2021-04-07	
J	BRAUTIGAM, M; KLEIN, M; KNIPPERS, R; MULLER, DG				BRAUTIGAM, M; KLEIN, M; KNIPPERS, R; MULLER, DG			INHERITANCE AND MEIOTIC ELIMINATION OF A VIRUS GENOME IN THE HOST ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	JOURNAL OF PHYCOLOGY			English	Article						ECTOCARPUS SILICULOSUS; ESV; LATENCY; MARINE DNA VIRUS; MEIOSIS; PCR; PHAEOPHYCEAE; SPECIFICITY; VIRUS DETECTION	DNA; EXTRACTION; AMPLIFICATION; RHODOPHYTA; GENES; ALGAE	The marine brown alga Ectocarpus siliculosus (Dillwyn) Lyngbye is frequently infected by a latent DNA virus that multiplies in modified sporangia and gametangia of the host. We describe a polymerase chain reaction (PCR) procedure for the amplification and detection of viral DNA in Ectocarpus. PCR analysis of parents and progeny plants confirmed that virus DNA passes through meiosis like a Mendelian trait. An infected sporophyte produced equal numbers of gametophytes with and without the viral genome. Thus, meiosis in sexual populations of the host acts as a mechanism for the creation of virus-free progeny.	UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY							BARKER PE, 1982, CANCER GENET CYTOGEN, V5, P81, DOI 10.1016/0165-4608(82)90043-7; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; GOFF LJ, 1993, J PHYCOL, V29, P381, DOI 10.1111/j.0022-3646.1993.00381.x; HONG YK, 1992, J PHYCOL, V28, P717, DOI 10.1111/j.0022-3646.1992.00717.x; JOHN ME, 1992, NUCLEIC ACIDS RES, V20, P2381, DOI 10.1093/nar/20.9.2381; KLEIN M, 1995, VIROLOGY, V206, P520, DOI 10.1016/S0042-6822(95)80068-9; KLEIN M, 1994, VIROLOGY, V202, P1076, DOI 10.1006/viro.1994.1443; KUHLENKAMP R, 1994, BOT MAR, V37, P525, DOI 10.1515/botm.1994.37.6.525; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MAYES C, 1992, J PHYCOL, V28, P712, DOI 10.1111/j.0022-3646.1992.00712.x; MIZIORKO HM, 1983, ANNU REV BIOCHEM, V52, P507, DOI 10.1146/annurev.bi.52.070183.002451; Muller D. G., 1991, JPN J PHYCOL, V39, P151; MULLER DG, 1995, J PHYCOL, V31, P173, DOI 10.1111/j.0022-3646.1995.00173.x; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1994, EUR J PHYCOL, V29, P219, DOI 10.1080/09670269400650671; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; PARODI ER, 1994, EUR J PHYCOL, V29, P113, DOI 10.1080/09670269400650561; REANNEY DC, 1974, INT REV CYTOL, V37, P21, DOI 10.1016/S0074-7696(08)61356-X; Sambrook J, 1989, MOL CLONING LABORATO; STARK GR, 1984, ANNU REV BIOCHEM, V53, P447, DOI 10.1146/annurev.bi.53.070184.002311; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; VALENTIN K, 1990, PLANT MOL BIOL, V15, P575, DOI 10.1007/BF00017832; WALSH PS, 1991, BIOTECHNIQUES, V10, P506, DOI 10.2144/000114018	27	34	34	0	1	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	OCT	1995	31	5					823	827		10.1111/j.0022-3646.1995.00823.x			5	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	TD903	WOS:A1995TD90300021					2021-04-07	
J	BOLAND, W; POHNERT, G; MAIER, I				BOLAND, W; POHNERT, G; MAIER, I			BIOSYNTHESIS OF ALGAE PHEROMONES .4. PERICYCLIC-REACTIONS IN NATURE - SPONTANEOUS COPE REARRANGEMENT INACTIVATES ALGAE PHEROMONES	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH			English	Article						BIOSYNTHESES; COPE REARRANGEMENTS; PERICYCLIC REACTIONS; PHEROMONES	MARINE BROWN-ALGAE; ECTOCARPUS-SILICULOSUS; COMPARATIVE RECEPTOR; CUTLERIA-MULTIFIDA; PHAEOPHYCEAE; ATTRACTANT		UNIV KONSTANZ,FAK BIOL,W-7750 CONSTANCE,GERMANY	BOLAND, W (corresponding author), UNIV BONN,INST ORGAN CHEM & BIOCHEM,GERHARD DOMAGK STR 1,D-53121 BONN,GERMANY.		Boland, Wilhelm/K-7762-2012; Pohnert, Georg/D-3721-2013	Boland, Wilhelm/0000-0001-6784-2534; Pohnert, Georg/0000-0003-2351-6336			BOLAND W, 1984, EUR J BIOCHEM, V144, P169, DOI 10.1111/j.1432-1033.1984.tb08445.x; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLAND W, 1980, Z NATURFORSCH C, V36, P262; Boland W, 1995, P NATL ACAD SCI USA, V92, P31; BROWN JM, 1979, J CHEM SOC P2, P436; MAIER I, 1993, PLANT CELL ENVIRON, V16, P891, DOI 10.1111/j.1365-3040.1993.tb00513.x; MAIER I, 1994, BOT ACTA, V107, P451, DOI 10.1111/j.1438-8677.1994.tb00820.x; MARNER FJ, 1984, Z NATURFORSCH C, V39, P689; MOORE RE, 1977, ACCOUNTS CHEM RES, V10, P40, DOI 10.1021/ar50110a002; MULLER DG, 1979, NATURE, V279, P430, DOI 10.1038/279430a0; MULLER DG, 1981, SCIENCE, V212, P1040, DOI 10.1126/science.212.4498.1040; MULLER DG, 1982, NATURWISSENSCHAFTEN, V69, P290, DOI 10.1007/BF00396442; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; NEUMANN C, 1990, EUR J BIOCHEM, V191, P453, DOI 10.1111/j.1432-1033.1990.tb19143.x; PANTKE S, 1992, THESIS U KARLSRUHE; POHNERT G, 1994, TETRAHEDRON, V50, P10235, DOI 10.1016/S0040-4020(01)81756-7; POHNERT G, 1995, IN PRESS TETRAHEDRON; SCHNEIDER M, 1975, ANGEW CHEM INT EDIT, V14, P707, DOI 10.1002/anie.197507071; STRATMANN K, 1992, ANGEW CHEM INT EDIT, V31, P1246, DOI 10.1002/anie.199212461; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; STRATMANN K, 1992, ANGEW CHEM, V104, P1261; SWINBOURNE ES, 1960, J CHEM SOC, V473, P2371; WIRTH D, 1992, HELV CHIM ACTA, V75, P734, DOI 10.1002/hlca.19920750309	23	24	24	0	18	VCH PUBLISHERS INC	DEERFIELD BEACH	303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788	0570-0833			ANGEW CHEM INT EDIT	Angew. Chem.-Int. Edit. Engl.	AUG 18	1995	34	15					1602	1604		10.1002/anie.199516021			3	Chemistry, Multidisciplinary	Chemistry	RT726	WOS:A1995RT72600014					2021-04-07	
J	CHEN, F; SUTTLE, CA				CHEN, F; SUTTLE, CA			AMPLIFICATION OF DNA-POLYMERASE GENE FRAGMENTS FROM VIRUSES INFECTING MICROALGAE	APPLIED AND ENVIRONMENTAL MICROBIOLOGY			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; CHAIN-REACTION; MICROMONAS-PUSILLA; PCR PRODUCTS; MARINE; ALGAE; ENTEROVIRUSES; PHYTOPLANKTON; COMPILATION; ALIGNMENT	Nested PCR with three highly degenerate primers was used for amplification and identification of DNA polymerase (pol) genes from viruses which infect three genera of microalgae. Group specific primers (AVS1 and AVS2) were designed on the basis of inferred amino acid sequences unique to the DNA pol genes of viruses (PBCV-1 and NY-2A) that infect an endosymbiotic Chlorella-like alga (Chlorophyceae) and a virus (MpV-SP1) which infects the photosynthetic flagellate Micromonas pusilla (Prasinophyceae). In addition, a nested primer (POL) was designed on the basis of the highly conserved amino acid sequence YGDTDS found in most B-family (alpha-like) DNA pol genes. These primers were used to amplify DNA from the three viruses, PBCV-1, NY-2A, and MpV-SP1, for which the primers were designed, as well as eight clonal isolates of genetically distinct viruses which infect M. pusilla and others which infect Chrysochromulina spp. (Prymnesiophyceae), suggesting that these are a group of related viruses. In contrast, no product resulted from using DNA from viruses which infect the marine brown algae Ectocarpus siliculosis and Feldmannia sp. (Phaeophyceae), suggesting that these viruses may not be closely related to those that infect microalgae. These primers were also used to amplify DNA from natural virus communities. Our results indicate that nested PCR, even under low-stringency conditions, can be used as a rapid method to verify the presence in seawater of a group of related viruses which infect microalgae. Sequence analysis of these fragments should provide information on the genetic diversity and potentially the phyletic relationships among these viruses. This is the first example of a PCR-based technique designed to detect viruses which infect eukaryotic algae.	UNIV TEXAS,DEPT MARINE SCI,PORT ARANSAS,TX 78373			Chen, Feng/P-3088-2014; Suttle, Curtis A/C-3150-2008; suttle, c/N-1144-2019	Suttle, Curtis A/0000-0002-0372-0033; suttle, c/0000-0002-0372-0033			ALBERT J, 1990, J CLIN MICROBIOL, V28, P1560, DOI 10.1128/JCM.28.7.1560-1564.1990; ANSARI SA, 1992, APPL ENVIRON MICROB, V58, P3984, DOI 10.1128/AEM.58.12.3984-3990.1992; ARAKAWA CK, 1990, DIS AQUAT ORGAN, V8, P165, DOI 10.3354/dao008165; BARTL S, 1994, BIOTECHNIQUES, V16, P246; BORSHEIM KY, 1993, FEMS MICROBIOL ECOL, V102, P141, DOI 10.1016/0378-1097(93)90197-A; BRAITHWAITE DK, 1993, NUCLEIC ACIDS RES, V21, P787, DOI 10.1093/nar/21.4.787; BRATBAK G, 1994, MICROBIAL ECOL, V28, P209, DOI 10.1007/BF00166811; CHEN F, 1994, 1994 ASLO PSA JOINT, pA16; Chen F., UNPUB; CHEN F, IN PRESS BIOTECHNIQU; COTTRELL MT, 1991, MAR ECOL PROG SER, V78, P1, DOI 10.3354/meps078001; COTTRELL MT, 1994, 1994 ASLO PSA JOINT, pA19; Fuhrman J., 1993, OCEANOGRAPHY, V6, P51, DOI 10.5670/oceanog.1993.14.; GONZALEZ GA, 1989, NATURE, V337, P749, DOI 10.1038/337749a0; GOULD SJ, 1989, P NATL ACAD SCI USA, V86, P1934, DOI 10.1073/pnas.86.6.1934; GRABHERR R, 1992, VIROLOGY, V188, P721, DOI 10.1016/0042-6822(92)90527-V; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; ITO J, 1991, NUCLEIC ACIDS RES, V19, P4045, DOI 10.1093/nar/19.15.4045; KAI M, 1991, NUCLEIC ACIDS RES, V19, P4562, DOI 10.1093/nar/19.16.4562; KNOTH K, 1988, NUCLEIC ACIDS RES, V22, P10932; KOPECKA H, 1993, APPL ENVIRON MICROB, V59, P1213, DOI 10.1128/AEM.59.4.1213-1219.1993; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MACK DH, 1988, P NATL ACAD SCI USA, V85, P6977, DOI 10.1073/pnas.85.18.6977; MAYER JA, 1979, NATURE, V281, P299, DOI 10.1038/281299a0; MAYER JA, 1978, THESIS U BRIT COLUMB; MEINTS RH, 1985, PLANT MOL BIOL REPT, V3, P180; MOLINA FI, 1994, BIOTECHNIQUES, V16, P998; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; PALENIK B, 1994, APPL ENVIRON MICROB, V60, P3212, DOI 10.1128/AEM.60.9.3212-3219.1994; PALENIK B, 1992, NATURE, V355, P265, DOI 10.1038/355265a0; PAUL JH, 1990, APPL ENVIRON MICROB, V56, P1963, DOI 10.1128/AEM.56.6.1963-1966.1990; PUIG M, 1994, APPL ENVIRON MICROB, V60, P2963, DOI 10.1128/AEM.60.8.2963-2970.1994; SUTTLE CA, 1994, MICROBIAL ECOL, V28, P237, DOI 10.1007/BF00166813; SUTTLE CA, 1990, NATURE, V347, P467, DOI 10.1038/347467a0; SUTTLE CA, 1991, APPL ENVIRON MICROB, V57, P721, DOI 10.1128/AEM.57.3.721-726.1991; SUTTLE CA, IN PRESS MAR ECOL PR; TSAI YL, 1993, APPL ENVIRON MICROB, V59, P3488, DOI 10.1128/AEM.59.10.3488-3491.1993; VANETTEN JL, 1983, SCIENCE, V219, P994, DOI 10.1126/science.219.4587.994; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VANETTEN JL, 1991, ARCH VIROL         S, V2, P137; WANG TSF, 1991, ANNU REV BIOCHEM, V60, P513, DOI 10.1146/annurev.bi.60.070191.002501; WATERS RE, 1982, J GEN VIROL, V63, P199, DOI 10.1099/0022-1317-63-1-199; ZEHR JP, 1989, APPL ENVIRON MICROB, V55, P2522, DOI 10.1128/AEM.55.10.2522-2526.1989; ZHANG YP, 1988, APPL ENVIRON MICROB, V54, P2170, DOI 10.1128/AEM.54.9.2170-2173.1988; ZHANG YP, 1994, 1994 ASLO PSA JOINT, pA85; ZIMMERMANN K, 1994, BIOTECHNIQUES, V17, P18; ZINTZ CB, 1991, BIOTECHNIQUES, V11, P158	49	105	110	0	16	AMER SOC MICROBIOLOGY	WASHINGTON	1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171	0099-2240			APPL ENVIRON MICROB	Appl. Environ. Microbiol.	APR	1995	61	4					1274	1278		10.1128/AEM.61.4.1274-1278.1995			5	Biotechnology & Applied Microbiology; Microbiology	Biotechnology & Applied Microbiology; Microbiology	QQ362	WOS:A1995QQ36200016	7747950	Bronze, Green Published			2021-04-07	
J	FIGUEROA, FL; AGUILERA, J; NIELL, FX				FIGUEROA, FL; AGUILERA, J; NIELL, FX			RED AND BLUE-LIGHT REGULATION OF GROWTH AND PHOTOSYNTHETIC METABOLISM IN PORPHYRA-UMBILICALIS (BANGIALES, RHODOPHYTA)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						ABSORPTANCE; GROWTH RATE; PACKAGE EFFECT; PHOTOSYNTHETIC PIGMENTS; PORPHYRA-UMBILICALIS; RED AND BLUE LIGHT	ACETABULARIA-MEDITERRANEA; ECTOCARPUS PHAEOPHYTA; CIRCADIAN-RHYTHM; FAST RESPONSES; ALGAE; ADAPTATION; APPARATUS; QUALITY; PLANTS; BROWN	Thallus discs 9 mm in diameter of Porphyra umbilicalis were cultivated for 4 weeks in either blue or red light at 50 mumol m-2 s-1 and 12 h light per day. Growth rate, in terms of weight and area, carbon content and dimensions of intercellular matrix, was higher in thalli grown in red light, while concentrations of soluble protein and photosynthetic pigments (chlorophyll, phycocyanin and phycoerythrin in particular), package effect and cell volume were higher in thalli grown in blue light. The slower growth rate in blue light was ascribed mainly to low efficiency of light absorption (high package effect) and low photosynthetic efficiency (in terms of total photosynthetic pigments) due to little overlap of photosystem I and photosystem II pigments in this red alga in blue light.		FIGUEROA, FL (corresponding author), UNIV MALAGA,FAC CIENCIAS,DEPT ECOL,CAMPUS UNIV TEATINOS,E-29071 MALAGA,SPAIN.		; Lopez Figueroa, Felix Diego/K-7720-2014	Aguilera, Jose/0000-0002-1911-111X; Lopez Figueroa, Felix Diego/0000-0003-3580-4693			BEER S, 1985, AUST J MAR FRESH RES, V36, P785; BEER S, 1983, J PHYCOL, V19, P516, DOI 10.1111/j.0022-3646.1983.00516.x; BERNER T, 1989, J PHYCOL, V25, P70, DOI 10.1111/j.0022-3646.1989.00070.x; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3; CLAUSS H, 1970, P177; DRING MJ, 1988, ANNU REV PLANT PHYS, V39, P157, DOI 10.1146/annurev.pp.39.060188.001105; DRING MJ, 1983, ENCY PLANT PHYSL B, V16, P545; FAUST MA, 1982, J PHYCOL, V18, P349, DOI 10.1111/j.1529-8817.1982.tb03195.x; FIGUEROA FL, 1994, 8 ACT S IB EST BENT, P42; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; Gantt E., 1990, BIOL RED ALGAE, P203; HAXO FT, 1950, J GEN PHYSIOL, V33, P389, DOI 10.1085/jgp.33.4.389; HUMPHREY GF, 1983, J EXP MAR BIOL ECOL, V66, P49, DOI 10.1016/0022-0981(83)90027-8; JEFFREY SW, 1975, BIOCHEM PHYSIOL PFL, V167, P191, DOI 10.1016/s0015-3796(17)30778-3; KIRK JTO, 1983, LIGHT PHOTOSYNTHESIS, P201; Kowallik W., 1987, BLUE LIGHT RESPONSES, VII, P7; LEUKART P, 1994, EUR J PHYCOL, V29, P103, DOI 10.1080/09670269400650551; Lichtenthaler H.K., 1984, CHLOROPLAST BIOGENES, P261; LOPEZ-FIGUEROA F, 1991, Scientia Marina, V55, P519; LOPEZFIGUEROA F, 1990, MAR BIOL, V104, P321, DOI 10.1007/BF01313274; LOPEZFIGUEROA F, 1989, PHYSIOL PLANTARUM, V76, P391; LUNING K, 1992, J PHYCOL, V28, P794, DOI 10.1111/j.0022-3646.1992.00794.x; LUNING K, 1985, MAR BIOL, V87, P119, DOI 10.1007/BF00539419; MATHIESON AC, 1975, J EXP MAR BIOL ECOL, V17, P137, DOI 10.1016/0022-0981(75)90027-1; MULLER S, 1976, Z PFLANZENPHYSIOL, V78, P461; OSBORNE BA, 1986, BIOL REV, V61, P1, DOI 10.1111/j.1469-185X.1986.tb00425.x; POLNEFULLER M, 1984, J PHYCOL, V20, P609, DOI 10.1111/j.0022-3646.1984.00609.x; ROBAINA R R, 1990, Journal of Applied Phycology, V2, P137, DOI 10.1007/BF00023375; RUDIGER W, 1992, PHOTOCHEM PHOTOBIOL, V55, P949, DOI 10.1111/j.1751-1097.1992.tb08542.x; RUYTERS G, 1987, BLUE LIGHT RESPONSES, V2, P71; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1991, PHOTOCHEM PHOTOBIOL, V53, P347, DOI 10.1111/j.1751-1097.1991.tb03639.x; SENGE M, 1991, BOT ACTA, V104, P139, DOI 10.1111/j.1438-8677.1991.tb00209.x; SENGER H, 1987, PHOTOCHEM PHOTOBIOL, V45, P939, DOI 10.1111/j.1751-1097.1987.tb07905.x; Senger H., 1984, BLUE LIGHT EFFECTS B, P419; SENGER H, 1987, BLUE LIGHT RESPONSES, V1, P75; SOKAL PR, 1981, J PHYCOL S, V23, P1; WADA M, 1989, ANNU REV PLANT PHYS, V40, P169; WENNICKE H, 1987, PLANT PHYSIOL, V84, P1252, DOI 10.1104/pp.84.4.1252	40	69	74	0	16	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	FEB	1995	30	1					11	18		10.1080/09670269500650761			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	QJ401	WOS:A1995QJ40100002		Bronze			2021-04-07	
J	KLEIN, M; LANKA, STJ; KNIPPERS, R; MULLER, DG				KLEIN, M; LANKA, STJ; KNIPPERS, R; MULLER, DG			COAT PROTEIN OF THE ECTOCARPUS-SILICULOSUS VIRUS	VIROLOGY			English	Article							POLYACRYLAMIDE GELS; ALGAL VIRUS; PHAEOPHYCEAE; GENOME; INFECTION; SEQUENCE; CLONING	Ectocarpus siliculosus virus, EsV, multiplies in sporangia and gametangia of the marine brown alga Ectocarpus siliculosus. We describe an improved method for the isolation of morphologically intact and infectious virus from diseased plants. We show that treatment of virus particles with high concentrations of CsCl results in a substantial loss of structural proteins. One of the proteins which resists CsCl treatment is glycoprotein-l, the largest of the three viral glycoproteins. We have isolated an EsV genomic fragment with an open reading frame encoding glycoprotein-1. The predicted amino acid sequence is rich in hydrophilic amino acids, but contains hydrophobic regions close to the amino and carboxy termini. A discrepancy between the molecular weight predicted from the coding region and the molecular weight determined by gel electrophoresis suggests that proteolytic processing is required for the maturation of the protein. (C) 1995 academic Press, Inc.	UNIV KONSTANZ,DIV BIOL,D-78434 CONSTANCE,GERMANY							BRUMMENDORF T, 1989, NEURON, V2, P1351, DOI 10.1016/0896-6273(89)90073-1; CHOU PY, 1978, ANNU REV BIOCHEM, V47, P251, DOI 10.1146/annurev.bi.47.070178.001343; DUBOCHET J, 1982, ADV OPTICAL ELECTRON, V8, P107; FRIESSKLEBL AK, 1994, J PHYCOL, V30, P663; GERSHONI JM, 1985, ANAL BIOCHEM, V146, P59, DOI 10.1016/0003-2697(85)90395-1; GRAVES MV, 1992, VIROLOGY, V188, P198, DOI 10.1016/0042-6822(92)90750-J; HAHN YS, 1988, VIROLOGY, V162, P167, DOI 10.1016/0042-6822(88)90406-0; HARLOW E, 1988, ANTIBODIES LABORATOR; HART GW, 1979, J BIOL CHEM, V254, P9747; HASCHEME.RH, 1970, ADV ENZYMOL REL S BI, V33, P71; HEINE VG, 1986, NUCLEIC ACIDS RES, V14, P4683; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; KRAUSSLICH HG, 1988, ANNU REV BIOCHEM, V57, P701, DOI 10.1146/annurev.bi.57.070188.003413; LAEMMLI UK, 1974, NATURE, V227, P680; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MATAGNE A, 1991, BIOCHEM J, V280, P553, DOI 10.1042/bj2800553; MIURA M, 1991, FEBS LETT, V289, P91, DOI 10.1016/0014-5793(91)80915-P; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; OFFERMANNSTEINH.K, 1990, NUCLEIC ACIDS RES, V18, P6452, DOI 10.1093/nar/18.21.6452; Sambrook J, 1989, MOL CLONING LABORATO; SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463, DOI 10.1073/pnas.74.12.5463; SCHAGGER H, 1987, ANAL BIOCHEM, V166, P368, DOI 10.1016/0003-2697(87)90587-2; SCHUSTER AM, 1990, VIROLOGY, V176, P515, DOI 10.1016/0042-6822(90)90021-I; STARR RC, 1993, J PHYCOL, V29, P1, DOI 10.1111/j.0022-3646.1993.00001.x; TAKAMIZAWA A, 1991, J VIROL, V65, P1105, DOI 10.1128/JVI.65.3.1105-1113.1991; TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P4350, DOI 10.1073/pnas.76.9.4350; WRAY W, 1981, ANAL BIOCHEM, V118, P197, DOI 10.1016/0003-2697(81)90179-2	31	21	22	0	2	ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS	SAN DIEGO	525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495	0042-6822			VIROLOGY	Virology	JAN 10	1995	206	1					520	526		10.1016/S0042-6822(95)80068-9			7	Virology	Virology	QD149	WOS:A1995QD14900056	7831806				2021-04-07	
J	BOLAND, W				BOLAND, W			THE CHEMISTRY OF GAMETE ATTRACTION - CHEMICAL STRUCTURES, BIOSYNTHESIS, AND (A)BIOTIC DEGRADATION OF ALGAL PHEROMONES	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA			English	Article; Proceedings Paper	Colloquium on Chemical Ecology - The Chemistry of Biotic Interaction	MAR 25-26, 1994	NATL ACAD SCI, WASHINGTON, DC	NATL ACAD SCI	NATL ACAD SCI		MARINE BROWN-ALGAE; ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; SENECIO-ISATIDEUS; ELECTROCYCLIC REACTIONS; ABSOLUTE-CONFIGURATION; CUTLERIA-MULTIFIDA; VOLATILE COMPOUNDS; C-11 HYDROCARBONS; SECRETION; PLANTS	Female gametes of marine brown algae release and/or attract their conspecific males by chemical signals. The majority of these compounds are unsaturated, nonfunctionalized acyclic, and/or alicyclic C-11 hydrocarbons, Threshold concentrations for release and attraction are generally observed in the range of 1-1000 pmol. The blends may contain various configurational isomers of the genuine pheromones as well as mixtures of enantiomers. Higher plants produce the C-11 hydrocarbons from dodeca-3,6,9-trienoic acid; brown algae exploit the family of icosanoids for biosynthesis of the same compounds. The biosynthetic routes comprise several spontaneously occurring pericyclic reactions such as [3.3]-sigmatropic rearrangements, [1.7]-hydrogen shifts, and electrocyclic ring closures. All pheromones are (a)biotically degraded by ubiquitous oxidative pathways involving singlet oxygen or hydroxyl radicals, which may be produced through the agency of heavy metals, huminic acids, or light.		BOLAND, W (corresponding author), INST ORGAN CHEM & BIOCHEM,GERHARD DOMAGK STR 1,D-53121 BONN,GERMANY.		Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			BANDARANAYAKE WM, 1980, J CHEM SOC CHEM COMM, P902, DOI 10.1039/c39800000902; BOHLMANN F, 1979, PHYTOCHEMISTRY, V18, P79, DOI 10.1016/S0031-9422(00)90920-8; BOLAND W, 1982, Z NATURFORSCH C, V37, P5; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLAND W, 1989, HELV CHIM ACTA, V72, P1288, DOI 10.1002/hlca.19890720616; BOLAND W, 1987, NATURWISSENSCHAFTEN, V74, P448, DOI 10.1007/BF00446105; BOLAND W, 1987, TETRAHEDRON LETT, V28, P307, DOI 10.1016/S0040-4039(00)95714-9; BOLAND W, 1985, EUR J BIOCHEM, V147, P83, DOI 10.1111/j.1432-1033.1985.tb08722.x; BOLAND W, 1987, EXPERIENTIA, V43, P466, DOI 10.1007/BF01940458; BOLAND W, 1981, LIEBIGS ANN CHEM, P2266; BOLAND W, 1980, Z NATURFORSCH C, V36, P262; BOLAND W, 1987, BIOL UNSERER ZEIT, V17, P176; DERENBACH JB, 1986, MAR CHEM, V19, P337, DOI 10.1016/0304-4203(86)90054-X; ERBES P, 1992, THESIS U KARLSRUHE K; FENICAL W, 1972, PHYTOCHEMISTRY, V11, P161; GARDNER HW, 1991, BIOCHIM BIOPHYS ACTA, V1084, P221, DOI 10.1016/0005-2760(91)90063-N; GROB K, 1976, J CHROMATOGR, V117, P285, DOI 10.1016/0021-9673(76)80005-2; HANEWALD KH, 1961, RECL TRAV CHIM PAY B, V80, P1003; HAY ME, 1988, MAR ECOL PROG SER, V48, P185, DOI 10.3354/meps048185; HUISGEN R, 1967, J AM CHEM SOC, V89, P7130, DOI 10.1021/ja01002a060; JAENICKE L, 1988, BOT ACTA, V101, P149, DOI 10.1111/j.1438-8677.1988.tb00026.x; JAENICKE L, 1982, ANGEW CHEM INT EDIT, V94, P643; JUTTNER F, 1984, LIMNOL OCEANOGR, V29, P1322; KAJIWARA T, 1991, PHYTOCHEMISTRY, V30, P1805, DOI 10.1016/0031-9422(91)85017-T; KAJIWARA T, 1993, ACS SYM SER, V525, P103; KEITEL J, 1990, HELV CHIM ACTA, V73, P2101, DOI 10.1002/hlca.19900730806; KOLLMANNSBERGER H, 1992, Chemie Mikrobiologie Technologie der Lebensmittel, V14, P81; MAIER I, 1993, PLANT CELL ENVIRON, V16, P891, DOI 10.1111/j.1365-3040.1993.tb00513.x; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MAIER I, 1982, PROTOPLASMA, V113, P137, DOI 10.1007/BF01282003; MAIER I, 1993, BOT ACTA, V106, P344, DOI 10.1111/j.1438-8677.1993.tb00759.x; MAIER I, 1987, ALGAL DEV MOL CELLUL, P66; MARNER FJ, 1984, Z NATURFORSCH C, V39, P689; McMurry T. J., 1986, CYTOCHROME P450 STRU, P1, DOI 10.1007/978-1-4757-9939-2_1; MOORE RE, 1973, J CHEM SOC CHEM COMM, P937, DOI 10.1039/c39730000937; MOORE RE, 1977, ACCOUNTS CHEM RES, V10, P40, DOI 10.1021/ar50110a002; MOPPER K, 1990, SCIENCE, V250, P661, DOI 10.1126/science.250.4981.661; MULLER DG, 1979, NATURE, V279, P430, DOI 10.1038/279430a0; MULLER DG, 1988, BIOL CHEM H-S, V369, P655, DOI 10.1515/bchm3.1988.369.2.655; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; MULLER DG, 1976, Z PFLANZENPHYSIOL, V80, P120; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; NEUMANN C, 1990, EUR J BIOCHEM, V191, P453, DOI 10.1111/j.1432-1033.1990.tb19143.x; PHILLIPS JA, 1990, PHYCOLOGIA, V29, P367, DOI 10.2216/i0031-8884-29-3-367.1; SCHMID CE, 1991, BIOL CHEM H-S, V372, P540; STRATMANN K, 1992, ANGEW CHEM INT EDIT, V31, P1246, DOI 10.1002/anie.199212461; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; STRATMANN K, 1992, THESIS U KARLSRUHE K; THOMAS BE, 1993, J AM CHEM SOC, V115, P4165, DOI 10.1021/ja00063a039; WIRTH D, 1992, HELV CHIM ACTA, V75, P734, DOI 10.1002/hlca.19920750309; WIRTH D, 1992, HELV CHIM ACTA, V75, P751, DOI 10.1002/hlca.19920750311	51	56	57	0	19	NATL ACAD SCIENCES	WASHINGTON	2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418	0027-8424			P NATL ACAD SCI USA	Proc. Natl. Acad. Sci. U. S. A.	JAN 3	1995	92	1					37	43		10.1073/pnas.92.1.37			7	Multidisciplinary Sciences	Science & Technology - Other Topics	QB238	WOS:A1995QB23800009	7816845	Green Published, Bronze			2021-04-07	
J	ERATA, M; KUBOTA, M; TAKAHASHI, T; INOUYE, I; WATANABE, M				ERATA, M; KUBOTA, M; TAKAHASHI, T; INOUYE, I; WATANABE, M			ULTRASTRUCTURE AND PHOTOTACTIC ACTION SPECTRA OF 2 GENERA OF CRYPTOPHYTE FLAGELLATE ALGAE, CRYPTOMONAS AND CHROOMONAS	PROTOPLASMA			English	Article						ACTION SPECTRUM; CHROOMONAS; CRYPTOMONAS; CRYPTOPHYTE; EYESPOT; PHOTOTAXIS; PHYCOBILIN	CHLAMYDOMONAS-REINHARDTII MUTANT; LASER SCANNING MICROSCOPY; ECTOCARPUS-SILICULOSUS; PHOTOPHOBIC RESPONSES; INDIVIDUAL CELLS; RHODOPSIN; LIGHT; PHOTORECEPTOR; CHROMOPHORE	A comparative action spectroscopical study was made on phototaxis in two genera of cryptomonads (cryptophyte flagellate algae), namely. Cryptomonas (rostratiformis) and Chroomonas (nordstedtii and coerulea). The two genera differ in their characteristic phycobilin pigmentation and. among three species. only Chroomonas coerulea possesses an eyespot. The two species with no eyespot, Cryptomonas rostratiformis and Chroomonas nordstedtii, exhibited positive phototaxis, showing very similar action spectra characterized by a broad band in the region from 450 nm to 650 nm. with an action maximum at about 560 nm: these features are essen tially the same as those observed previously for Cryptomonas strain CR-1. In Cryptomonas rostratiformis, a small peak was also found at 280 nm in the UV-B/C region. Chroomonas coerulea, with eyespot, did not exhibit distinct positive phototaxis in a wide spectral region at any given. even very low. light intensity, but exhibited negative phototaxis of spectral sensitivity maximal at 400-450 nm. These results indicate that the positive phototaxis of Cryptomonas (rostratiformis mis and CR-1) and Chroomonas nordstedtii is mediated by the same, yet unidentified photoreceptor(s). Chroomonas nordstedtii, possessing no phycoerythrin absorbing at 545 nm, also exhibits positive phototaxis at ca. 560 nm, and this result disfavors the so fur proposed possibility that the positive phototaxis of the cryptophytes may be mediated by phycobilin pigments. On the other hand. the spectral characteristics of negative phototaxis of Chroomonas coerulea can possibly be ascribed to the presence of an eyespot.	OKAZAKI NATL RES INST,NATL INST BASIC BIOL,OKAZAKI,AICHI 444,JAPAN; UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN; JAPAN ADV INST SCI & TECHNOL,SCH MAT SCI,TATSUNOKUCHI,ISHIKAWA,JAPAN							BRODHUN B, 1990, PHOTOCHEM PHOTOBIOL, V52, P865, DOI 10.1111/j.1751-1097.1990.tb08695.x; ERATA M, 1989, BOT MAG TOKYO, V102, P429, DOI 10.1007/BF02488125; ERATA M, 1991, Journal of Japanese Botany, V66, P7; Erata M, 1987, B SUGADAIRA MONT RES, V8, P57; FOSTER KW, 1984, NATURE, V311, P756, DOI 10.1038/311756a0; FOSTER KW, 1980, MICROBIOL REV, V44, P572, DOI 10.1128/MMBR.44.4.572-630.1980; HALLDAL PER, 1958, PHYSIOL PLANTARUM, V11, P118; HASHIMOTO T, 1982, ANN M JAP SOC PLANT, P38; HEGEMANN P, 1991, BIOPHYS J, V60, P1477, DOI 10.1016/S0006-3495(91)82183-X; HILL DRA, 1991, J PHYCOL, V27, P133, DOI 10.1111/j.0022-3646.1991.00133.x; HILL DRA, 1989, PHYCOLOGIA, V28, P455, DOI 10.2216/i0031-8884-28-4-455.1; KANEDA H, 1986, PLANT CELL PHYSIOL, V27, P265; KANEDA H, 1987, PLANT PHYSIOL, V84, P178, DOI 10.1104/pp.84.1.178; KATO S, 1981, JAP J PHYCOL, V30, P63; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KREIMER G, 1994, INT REV CYTOL, V148, P229, DOI 10.1016/S0074-7696(08)62409-2; KREIMER G, 1990, EUR J CELL BIOL, V53, P101; KREIMER G, 1991, EUR J CELL BIOL, V55, P318; LAWSON MA, 1991, BIOPHYS J, V60, P1490, DOI 10.1016/S0006-3495(91)82184-1; LUCAS IAN, 1982, BRIT PHYCOL J, V17, P13, DOI 10.1080/00071618200650031; MacColl R., 1987, PHYCOBILIPROTEINS; Melkonian M., 1984, Progress phycol. Res., V3, P193; NULTSCH W, 1988, PHOTOCHEM PHOTOBIOL, V47, P837, DOI 10.1111/j.1751-1097.1988.tb01668.x; PROVASOLI L, 1959, ECOLOGY ALGAE, P84; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; TAKAHASHI T, 1992, FEBS LETT, V314, P275, DOI 10.1016/0014-5793(92)81488-8; TAKAHASHI T, 1991, BIOCHEM BIOPH RES CO, V178, P1273, DOI 10.1016/0006-291X(91)91031-7; TAKAHASHI T, 1982, CELL STRUCT FUNCT, V7, P183, DOI 10.1247/csf.7.183; UEMATSUKANEDA H, 1982, PHYSIOL PLANTARUM, V56, P194, DOI 10.1111/j.1399-3054.1982.tb00324.x; WATANABE M, 1976, PLANT CELL PHYSIOL, V17, P683, DOI 10.1093/oxfordjournals.pcp.a075324; WATANABE M, 1982, PHOTOCHEM PHOTOBIOL, V35, P559, DOI 10.1111/j.1751-1097.1982.tb02609.x; WATANABE M, 1974, PLANT CELL PHYSIOL, V15, P413; WATANABE M, 1982, PHOTOCHEM PHOTOBIOL, V36, P491, DOI 10.1111/j.1751-1097.1982.tb04407.x; WATANABE M, 1978, PLANT PHYSIOL, V61, P816, DOI 10.1104/pp.61.5.816; Watanabe M, 1995, CRC HDB ORGANIC PHOT, P1260	37	14	14	0	8	SPRINGER-VERLAG WIEN	VIENNA	SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA	0033-183X			PROTOPLASMA	Protoplasma		1995	188	3-4					258	266		10.1007/BF01280378			9	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	TC735	WOS:A1995TC73500014					2021-04-07	
J	SCHNEPF, E				SCHNEPF, E			A PHAGOMYXA-LIKE ENDOPARASITE OF THE CENTRIC MARINE DIATOM BELLEROCHEA-MALLEUS - A PHAGOTROPHIC PLASMODIOPHOROMYCETE	BOTANICA ACTA			English	Article						PHAGOMYXA; PLASMODIOPHOROMYCETES; ULTRASTRUCTURE; FLAGELLAR APPARATUS; ZOOSPORE RELEASE; PARASITE OF MARINE DIATOMS	ULTRASTRUCTURE	In September 1993 the marine centric diatom, Bellerochea malleus (Brightwell) Heurck, collected in the Wadden Sea near List/Sylt, was parasitized by a Phagomyxa algarum-like organism. Karling (1944) reported Phagomyxa algarum Karling in North Carolina as a parasite of the filamentous brown algae Ectocarpus and Pylaiella. The Bellerochea parasite develops an endocytoplasmic plasmodium and incorporates host cytoplasm into a large, central digestion vacuole, by a form of phagocytosis. Later on, the plasmodium cleaves to form a zoosporangiosorus. Each zoosporangium is surrounded by a thin wall. It releases zoospores (2.5 x 4 mu m) with two unequal flagella, an anterior (4 mu m long) and a posterior (8 mu m long). Cystosori and cysts could not be detected. The ultrastructure of the zoosporangia and zoospores was investigated, with particular attention to the flagellar apparatus and its rearrangement during zoospore release. This process is very similar to that recorded for zoospores of the plasmodiophoromycete Polymyxa graminis Ledigham (Barr and Allan, 1982). The Bellerochea parasite is closely related to or identical with Phagomyxa algarum. The taxonomic position of Phagomyxa is discussed. In spite of its phagotrophic nutrition and the possible lack of cystosori and cysts, Phagomyxa should be regarded as a member of the Plasmodiophoromycota (or Plasmodiophorida) but not included in a separate order Phagomyxida as proposed by Cavalier-Smith (1993a).	WATTENMEERSTN LIST,BIOL ANSTALT HELGOLAND,SYLT,GERMANY	SCHNEPF, E (corresponding author), UNIV HEIDELBERG,NEUENHEIMER FELD 230,D-69120 HEIDELBERG,GERMANY.						BARR DJS, 1982, CAN J BOT, V60, P2496, DOI 10.1139/b82-302; CAVALIERSMITH T, 1993, MICROBIOL REV, V57, P953, DOI 10.1128/MMBR.57.4.953-994.1993; CAVALIERSMITH T, 1993, J EUKARYOT MICROBIOL, V40, P609, DOI 10.1111/j.1550-7408.1993.tb06117.x; DAMBRA V, 1977, CAN J BOT, V55, P831, DOI 10.1139/b77-098; Drebes G, 1974, MARINES PHYTOPLANKTO; Dylewski D.P., 1990, P399; DYLEWSKI DP, 1978, AM J BOT, V65, P258, DOI 10.2307/2442265; Karling JS, 1944, AM J BOT, V31, P38, DOI 10.2307/2437666; KRISTIANSEN J, 1993, ARCH PROTISTENKD, V143, P195, DOI 10.1016/S0003-9365(11)80288-5; MILLER CE, 1984, CAN J BOT, V63, P263; Porter D., 1986, P141; PORTER D, 1987, FRONTIERS APPLIED MI, V2, P235; SCHNEPF E, 1994, ARCH PROTISTENKD, V144, P63, DOI 10.1016/S0003-9365(11)80225-3; Sparrow F.K., 1960, AQUATIC PHYCOMYCETES	14	14	14	0	5	GEORG THIEME VERLAG	STUTTGART	P O BOX 30 11 20, D-70451 STUTTGART, GERMANY	0932-8629			BOT ACTA	Bot. Acta	DEC	1994	107	6					374	382		10.1111/j.1438-8677.1994.tb00810.x			9	Plant Sciences	Plant Sciences	PY938	WOS:A1994PY93800002					2021-04-07	
J	MAIER, I; CALENBERG, M				MAIER, I; CALENBERG, M			EFFECT OF EXTRACELLULAR CA2+ AND CA2+-ANTAGONISTS ON THE MOVEMENT AND CHEMOORIENTATION OF MALE GAMETES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	BOTANICA ACTA			English	Article						ECTOCARPUS; PHAEOPHYCEAE; FLAGELLAR MOVEMENT; PHEROMONE; CHEMOTAXIS; CALCIUM ANTAGONISTS; SIGNAL TRANSDUCTION	SEA-URCHIN SPERM; COMPARATIVE RECEPTOR; CRITHIDIA-ONCOPELTI; CUTLERIA-MULTIFIDA; CALCIUM CHANNELS; CILIARY MOVEMENT; BROWN ALGA; CALMODULIN; CHLAMYDOMONAS; FLAGELLA	Chemoorientation in male gametes of Ectocarpus siliculosus in response to sexual pheromones is effected by two distinct mechanisms, chemokinesis and chemoklinotaxis. These are characterized by a strongly asymmetric bending pattern of the anteriorly-directed flagellum and transient; unilateral bending of the hind flagellum, respectively. Removal of extracellular Ca2+ showed that normal flagellar movement and chemokinesis require millimolar concentrations of Ca2+ in the medium. The response to pheromones is strongly inhibited by La3+, whereas the Ca2+-channel drugs, verapamil and nifedipine, have only little effect. Nifedipine nethertheless effectively inhibited accumulation at pheromone sources. These results are interpreted as an indication for the involvement of two pharmacologically distinct Ca2+-channels in chemokinesis and chemoklinotaxis. The calmodulin-antagonist, trifluoperazine, induces, at low concentrations, the same flagellar response in chemokinesis as the pheromone, the mechanism of action remaining unknown.	UNIV COLOGNE, INST BOT, W-5000 COLOGNE, GERMANY	MAIER, I (corresponding author), UNIV KONSTANZ, FAK BIOL, POSTFACH 5560, D-78464 CONSTANCE, GERMANY.						ADUNYAH ES, 1982, FEBS LETT, V143, P65, DOI 10.1016/0014-5793(82)80274-3; ANDRIVON C, 1988, BIOL CELL, V63, P133, DOI 10.1016/0248-4900(88)90052-4; BESSEN M, 1980, J CELL BIOL, V86, P446, DOI 10.1083/jcb.86.2.446; BOLAND W, 1984, EUR J BIOCHEM, V144, P169, DOI 10.1111/j.1432-1033.1984.tb08445.x; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BROKAW CJ, 1991, CELL MOTIL CYTOSKEL, V18, P123, DOI 10.1002/cm.970180207; BROKAW CJ, 1979, J CELL BIOL, V82, P401, DOI 10.1083/jcb.82.2.401; BROKAW CJ, 1985, J CELL BIOL, V100, P1875, DOI 10.1083/jcb.100.6.1875; BROKAW CJ, 1974, BIOCHEM BIOPH RES CO, V58, P795, DOI 10.1016/S0006-291X(74)80487-0; BROKAW CJ, 1974, J CELL PHYSIOL, V83, P151, DOI 10.1002/jcp.1040830118; BROWNLEE C, 1988, J CELL SCI, V91, P249; CALDWELL P C, 1970, P10; CARAFOLI E, 1992, J BIOL CHEM, V267, P2115; CATTERALL WA, 1992, TRENDS PHARMACOL SCI, V13, P256, DOI 10.1016/0165-6147(92)90079-L; CHARUK JHM, 1990, ANAL BIOCHEM, V188, P123, DOI 10.1016/0003-2697(90)90539-L; DETMERS PA, 1986, EXP CELL RES, V163, P317, DOI 10.1016/0014-4827(86)90063-7; ECKERT R, 1972, SCIENCE, V176, P473, DOI 10.1126/science.176.4034.473; GELLER A, 1981, J EXP BIOL, V92, P53; HOHFELD I, 1988, PROTOPLASMA, V147, P16, DOI 10.1007/BF01403874; HOLWILL MEJ, 1975, NATURE, V255, P157, DOI 10.1038/255157a0; HOLWILL MEJ, 1976, J EXP BIOL, V65, P229; HYAMS JS, 1978, J CELL SCI, V33, P235; KAMIYA R, 1984, J CELL BIOL, V98, P97, DOI 10.1083/jcb.98.1.97; KATSAROS CI, 1993, J PHYCOL, V29, P787, DOI 10.1111/j.0022-3646.1993.00787.x; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KLEE CB, 1982, ADV PROTEIN CHEM, V35, P213, DOI 10.1016/S0065-3233(08)60470-2; LANSMAN JB, 1986, J GEN PHYSIOL, V88, P321, DOI 10.1085/jgp.88.3.321; LEVIN RM, 1977, MOL PHARMACOL, V13, P690; MAIER I, 1993, PLANT CELL ENVIRON, V16, P891, DOI 10.1111/j.1365-3040.1993.tb00513.x; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MARSHAK DR, 1981, P NATL ACAD SCI-BIOL, V78, P6793, DOI 10.1073/pnas.78.11.6793; Martell A. E., 1971, SPECIAL PUBLICATION, V17; MARTIN SR, 1985, EUR J BIOCHEM, V151, P543, DOI 10.1111/j.1432-1033.1985.tb09137.x; MATSUOKA T, 1991, EUR J PROTISTOL, V27, P371, DOI 10.1016/S0932-4739(11)80254-3; MCFADDEN GI, 1987, J CELL BIOL, V105, P903, DOI 10.1083/jcb.105.2.903; MEANS AR, 1988, RECENT PROG HORM RES, V44, P223; Melkonian M., 1992, P179; Miller R.L., 1985, P275; MOGAMI Y, 1990, CELL CALCIUM, V11, P665, DOI 10.1016/0143-4160(90)90021-L; MOORE PB, 1982, J BIOL CHEM, V257, P9663; MULLER DG, 1976, J PHYCOL, V12, P252, DOI 10.1111/j.0022-3646.1976.00252.x; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1976, ARCH MICROBIOL, V109, P89, DOI 10.1007/BF00425117; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; MULLER DG, 1985, Z NATURFORSCH C, V40, P457; MULLER DG, 1976, Z PFLANZENPHYSIOL, V80, P120; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; NAITOH Y, 1972, SCIENCE, V176, P523, DOI 10.1126/science.176.4034.523; NAKAZAWA K, 1993, BRIT J PHARMACOL, V109, P137, DOI 10.1111/j.1476-5381.1993.tb13543.x; OMOTO CK, 1985, CELL MOTIL CYTOSKEL, V5, P53, DOI 10.1002/cm.970050105; OTTER T, 1989, CELL MOVEMENT, V1, P281; POMMERVILLE JC, 1990, J CHEM ECOL, V16, P121, DOI 10.1007/BF01021274; REIZE IB, 1989, BOT ACTA, V102, P145, DOI 10.1111/j.1438-8677.1989.tb00083.x; ROUFOGALIS BD, 1982, CALCIUM CELL FUNCTIO, V3, P129; Salisbury J.L., 1992, P393; SANGUINETTI MC, 1990, AM J PHYSIOL, V259, pH1881; SCHACKMANN RW, 1986, J BIOL CHEM, V261, P8719; SCHMIDT JA, 1976, NATURE, V262, P713, DOI 10.1038/262713a0; SERRES C, 1991, CELL MOTIL CYTOSKEL, V18, P228, DOI 10.1002/cm.970180308; SILLEN LG, 1964, SPECIAL PUBLICATION, V17; SJOBLAD RD, 1981, MOL CELL BIOL, V1, P1057, DOI 10.1128/MCB.1.12.1057; SORDAHL LA, 1975, ARCH BIOCHEM BIOPHYS, V167, P104, DOI 10.1016/0003-9861(75)90446-4; SPEDDING M, 1992, PHARMACOL REV, V44, P363; TAYLOR A, 1993, PLANTA, V189, P109; VANHOUTEN J, 1994, TRENDS NEUROSCI, V17, P62, DOI 10.1016/0166-2236(94)90076-0; VANHOUTEN JL, 1991, SENSORY RECEPTORS SI, P65; WARD GE, 1985, J CELL BIOL, V101, P2324, DOI 10.1083/jcb.101.6.2324; WATANABE Y, 1990, CALCIUM INTRACELLULA, P343; ZIMMER M, 1987, EUR J BIOCHEM, V164, P411, DOI 10.1111/j.1432-1033.1987.tb11073.x	70	67	68	0	16	GEORG THIEME VERLAG KG	STUTTGART	RUDIGERSTR 14, D-70469 STUTTGART, GERMANY	0932-8629			BOT ACTA	Bot. Acta	DEC	1994	107	6					451	460		10.1111/j.1438-8677.1994.tb00820.x			10	Plant Sciences	Plant Sciences	PY938	WOS:A1994PY93800012					2021-04-07	
J	WINTER, C; WINTER, M; POHL, P				WINTER, C; WINTER, M; POHL, P			CADMIUM ADSORPTION BY NONLIVING BIOMASS OF THE SEMI-MACROSCOPIC BROWN ALGA, ECTOCARPUS-SILICULOSUS, GROWN IN AXENIC MASS-CULTURE AND LOCALIZATION OF THE ADSORBED CD BY TRANSMISSION ELECTRON-MICROSCOPY	JOURNAL OF APPLIED PHYCOLOGY			English	Article						CADMIUM ADSORPTION; ECTOCARPUS SILICULOSUS; GROWTH CONDITIONS; ADSORPTION PARAMETERS; REGENERATION; ELECTRON MICROSCOPY	CHLORELLA-VULGARIS; BIOSORPTION; BIOSORBENTS; SORPTION; RECOVERY	Non-living, freeze-dried material of the brown alga Ectocarpus siliculosus (Phaeophyceae) demonstrated high equilibrium uptake of Cd from aqueous solutions (Fehrmann and Pohl, 1993). The alga was grown in 250-L photobioreactors under various growth conditions (light, salinity and nutrient concentrations) in order to obtain larger quantities of biomass and to improve its Cd adsorption capacity. To derive further knowledge on the biosorbant phenomenon different adsorption parameters such as pH for the sorption process and kinetics of Cd adsorption were tested. The maximum adsorption capacity of the freeze-dried biomass exceeded 41 mg Cd per g biomass. After repeated addition of low Cd concentrations the maximum adsorption capacity was lower (31.4 mg Cd per g biomass). In comparison with other adsorbing materials (activated carbon, silica gel, siliceous earth) E. siliculosus showed significantly higher adsorption capacity. Desorption of deposited Cd with 0.1 M HCl resulted in no changes of the adsorption capacity through five subsequent adsorption-/desorption-cycles. Hence, dried E. siliculosus appears to be an efficient material for the elimination of Cd from industrial waste water. Transmission electron microscopic investigations showed an electron dense area in the outer surface layers of the cell wall after Cd adsorption indicating the most likely location of Cd fixation.	CHRISTIAN ALBRECHTS UNIV KIEL,INST ANAT,D-24105 KIEL,GERMANY	WINTER, C (corresponding author), CHRISTIAN ALBRECHTS UNIV KIEL,INST PHARMAZEUT,PHARMAZEUT BIOL ABT,GUTENBERGSTR 76-78,D-24118 KIEL,GERMANY.						AKSU Z, 1990, ENVIRON TECHNOL, V11, P979, DOI 10.1080/09593339009384950; CONWAY HL, 1979, J FISH RES BOARD CAN, V36, P579, DOI 10.1139/f79-083; FEHRMANN C, 1993, J APPL PHYCOL, V5, P555, DOI 10.1007/BF02184634; FEHRMANN C, 1993, PHARMAZEUTISCHE ZEIT, V27, P2073; GEISWEID HJ, 1983, Z PFLANZENPHYSIOL, V109, P127, DOI 10.1016/S0044-328X(83)80202-5; GUTKNECHT JOHN, 1965, LIMNOL OCEANOGR, V10, P58; HARRIS PO, 1990, ENVIRON SCI TECHNOL, V242, P220; HOLAN ZR, 1993, BIOTECHNOL BIOENG, V41, P819, DOI 10.1002/bit.260410808; JEFFERS TH, 1991, RI9340 BUR MIN REP I; KHUMMONGKOL D, 1982, BIOTECHNOL BIOENGNG, V12, P2643; KINKADE ML, 1975, ENVIRON RES, V10, P308, DOI 10.1016/0013-9351(75)90091-2; KUYUCAK N, 1988, BIOTECHNOL LETT, V10, P137, DOI 10.1007/BF01024641; MARTINS LO, 1990, ENZYME MICROB TECHNO, V10, P794; POHL P, 1987, PHYTOCHEMISTRY, V266, P1657; Pohl P., 1988, ALGAL BIOTECHNOLOGY, P209; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; RUBIN AJ, 1974, AQUEOUS ENV CHEM MET, P255; RUCKER G, 1988, WISSENSCHAFTLICHE VE; SMITH JE, 1981, INT C, V3, P155; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; TING YP, 1989, BIOTECHNOL BIOENG, V34, P990, DOI 10.1002/bit.260340713; VOLESKY B, 1987, TRENDS BIOTECHNOL, V5, P96, DOI 10.1016/0167-7799(87)90027-8; XUE HB, 1988, WATER RES, V227, P917	23	11	11	0	9	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0921-8971			J APPL PHYCOL	J. Appl. Phycol.	DEC	1994	6	5-6					479	487		10.1007/BF02182402			9	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	QB595	WOS:A1994QB59500007					2021-04-07	
J	KUHLENKAMP, R; MULLER, DG				KUHLENKAMP, R; MULLER, DG			ISOLATION AND REGENERATION OF PROTOPLASTS FROM HEALTHY AND VIRUS-INFECTED GAMETOPHYTES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	BOTANICA MARINA			English	Article							BROWN ALGA; GROWTH-RATE; AGAR COMPOSITION; RHODOPHYTA; GIGARTINALES; STRAINS; CULTURE; GENOME	Up to 1.3 X 10(8) protoplasts per g fresh weight were obtained from healthy gametophytes of Ectocarpus with an enzyme solution containing cellulase and a crude extract of abalone acetone powder. Virus-infected gametophytes gave lower yields of protoplasts than the healthy material. Culture conditions and algal growth influenced protoplast yields: old cultures and slowly growing parts, such as cells of the main axis, produced few protoplasts. In contrast, cell walls of meristematic regions were easily digested. Protoplasts regenerated cell walls within a few days, and after 6-7 weeks the resulting mature plants were identical with their donor specimens. Regenerates from the normal gametophytes developed mature gametangia, whereas those from the virus-infected gametophytes produced pathological symptoms upon maturity. Gametangia or infection symptoms frequently occurred very early and before the regenerates established new filaments (neoteny). In contrast to zoids, protoplasts were not infected by freshly released virus particles. The results show that the viral genome is latently present in somatic cells of infected gametophytes, where it is duplicated during mitosis and eventually expressed upon maturity of the host.	UNIV KONSTANZ, FAK BIOL, D-78434 CONSTANCE, GERMANY							BJORK M, 1990, BOT MAR, V33, P433, DOI 10.1515/botm.1990.33.5.433; BOYEN C, 1988, PLANT PHYSIOL BIOCH, V26, P653; BURNS AR, 1984, BOT MAR, V27, P45, DOI 10.1515/botm.1984.27.2.45; BUTLER DM, 1989, J EXP BOT, V40, P1237, DOI 10.1093/jxb/40.11.1237; COCKING EC, 1972, ANN REV PLANT PHYSIO, V23, P29, DOI 10.1146/annurev.pp.23.060172.000333; DUCREUX G, 1988, PLANTA, V174, P25, DOI 10.1007/BF00394869; EKMAN P, 1990, BOT MAR, V33, P483, DOI 10.1515/botm.1990.33.6.483; Eriksson TR, 1985, PLANT PROTOPLASTS, P1; FISHER DD, 1987, PHYCOLOGIA, V26, P488, DOI 10.2216/i0031-8884-26-4-488.1; FITZSIMONS P J, 1985, P12; GOMEZPINCHETTI JL, 1993, MAR BIOL, V116, P553, DOI 10.1007/BF00355473; GOMEZPINCHETTI JL, 1993, PLANT CELL REP, V12, P541; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; HUGHES J, 1975, STAIN TECHNOL, V50, P319, DOI 10.3109/10520297509117082; KEVEKORDES K, 1993, J EXP BOT, V44, P1587, DOI 10.1093/jxb/44.10.1587; KLOAREG B, 1987, PLANT SCI, V50, P189, DOI 10.1016/0168-9452(87)90073-2; KLOAREG B, 1989, PLANT SCI, V62, P105, DOI 10.1016/0168-9452(89)90194-5; KLOAREG B, 1988, OCEANOGR MAR BIOL, V26, P259; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LEGALL Y, 1990, PLANT CELL REP, V8, P582, DOI 10.1007/BF00270058; LIGNELL A, 1989, BOT MAR, V32, P219, DOI 10.1515/botm.1989.32.3.219; MAEDA H, 1967, J BIOCHEM-TOKYO, V62, P276, DOI 10.1093/oxfordjournals.jbchem.a128660; MEJJAD M, 1992, PROTOPLASMA, V169, P42, DOI 10.1007/BF01343368; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1993, HYDROBIOLOGIA, V261, P37; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; POLNEFULLER M, 1990, J PHYCOL, V26, P674, DOI 10.1111/j.0022-3646.1990.00674.x; REDDY CRK, 1991, J APPL PHYCOL, V3, P265, DOI 10.1007/BF00003585; SAGA N, 1986, NOVA HEDWIGIA S, V83, P37; Starr R.C., 1987, Journal of Phycology, V23, P1; Uchida Takuji, 1992, Japanese Journal of Phycology, V40, P261; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	35	14	14	0	6	WALTER DE GRUYTER GMBH	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055	1437-4323		BOT MAR	Bot. Marina	NOV	1994	37	6					525	530		10.1515/botm.1994.37.6.525			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	PY562	WOS:A1994PY56200005					2021-04-07	
J	MULLER, DG; EICHENBERGER, W				MULLER, DG; EICHENBERGER, W			BETAINE LIPID-CONTENT AND SPECIES DELIMITATION IN ECTOCARPUS, FELDMANNIA AND HINCKSIA (ECTOCARPALES, PHAEOPHYCEAE)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						BETAINE LIPIDS; DGTA; CHEMOTAXONOMY; ECTOCARPUS; FELDMANNIA; HINCKSIA; PHAEOPHYCEAE	SILICULOSUS PHAEOPHYCEAE	The lipid composition of 140 clonal isolates of brown algae, covering 16 species of the genera Ectocarpus, Feldmannia and Hincksia, was analysed. All taxa contained the glycolipids monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol, and the phospholipids phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and an unidentified PX. Presence of the betaine lipid diacylglycerylhydroxymethyltrimethyl-beta-alanine (DGTA) was strongly correlated with certain species in the genera Hincksia and Ectocarpus. While seven species of Hincksia contained DGTA, this lipid was absent in H. granulosa and in an unidentified Hincksia species. Due to heteromorphy of generations, species assignments in the genus Ectocarpus were made only for isolates for which the complete life history was known. We found that E. fasciculatus contains DGTA, while this lipid is absent in E. siliculosus. A collection of 40 non-sexual Ectocarpus strains included representatives with and without DGTA. The value of DGTA as a taxonomic marker in Ectocarpus and Hincksia is discussed.	UNIV BERN,INST BIOCHEM,CH-3012 BERN,SWITZERLAND	MULLER, DG (corresponding author), UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY.						Abbott I. A., 1976, MARINE ALGAE CALIFOR; ADAMS GMW, 1976, GENETICS ALGAE, P69; Allen CF, 1971, METHODS ENZYMOLOGY A, V123, P523; ARDRE F, 1969, ACTA BIOLOGICA, V10, P137; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; CHEN PS, 1956, ANAL CHEM, V28, P1756, DOI 10.1021/ac60119a033; DITTMER JC, 1964, J LIPID RES, V5, P126; Douce R., 1980, BIOCH PLANTS LIPIDS, V4, P321; EICHENBERGER W, 1993, PHYTOCHEMISTRY, V34, P1323, DOI 10.1016/0031-9422(91)80024-U; EICHENBERGER W, 1992, METABOLISM, STRUCTURE AND UTILIZATION OF PLANT LIPIDS, P18; Harwood J. L., 1980, BIOCH PLANTS, V4, P301; HEINZ E, 1967, BIOCHIM BIOPHYS ACTA, V144, P333, DOI 10.1016/0005-2760(67)90162-2; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; MOTOMURA T, 1990, J PHYCOL, V26, P80, DOI 10.1111/j.0022-3646.1990.00080.x; MUDD JB, 1980, BIOCH PLANTS, V4, P249; Muller D. G., 1991, JPN J PHYCOL, V39, P151; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; MULLER DG, 1977, BRIT PHYCOL J, V12, P131; MULLER DG, 1972, PHYCOLOGIA, V11, P11; MUNIER R, 1951, B SOC CHIM BIOL, V33, P846; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; SCHMID CE, 1994, IN PRESS J PLANT PHY; Schneider C.W., 1991, SEAWEEDS SE US; SILVA PC, 1986, SMITHSONIAN CONTRIBU, V27, P127; SOUTH GR, 1980, MEMOIRS U NEWFOUNDLA, V3, P1; STACHE B, 1991, NATO ASI SERIES, V22; Starr R.C., 1987, Journal of Phycology, V23, P1; Taylor WR, 1960, MARINE ALGAE E TROPI; THIES W, 1971, Z PFLANZENZUCHTUNG, V65, P181; VOGEL G, 1990, CHEM PHYS LIPIDS, V52, P99, DOI 10.1016/0009-3084(90)90154-J; WOMERSLEY HBS, 1987, MARINE BENTHIC FLORA, V2	33	16	16	0	4	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	NOV	1994	29	4					219	225		10.1080/09670269400650671			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	PR227	WOS:A1994PR22700002		Bronze			2021-04-07	
J	ROBLEDO, DR; SOSA, PA; GARCIAREINA, G; MULLER, DG				ROBLEDO, DR; SOSA, PA; GARCIAREINA, G; MULLER, DG			PHOTOSYNTHETIC PERFORMANCE OF HEALTHY AND VIRUS-INFECTED FELDMANNIA-IRREGULARIS AND F-SIMPLEX (PHAEOPHYCEAE)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						FELDMANNIA; PATHOLOGY; PHAEOPHYCEAE; PHOTOSYNTHESIS; VIRUS INFECTION	ALGA ECTOCARPUS-SILICULOSUS; MARINE; GENOME	The filamentous brown algae Feldmannia simpler and F. irregularis are attacked by aquatic viruses, which propagate in deformed sporangia of the host plants. In order to evaluate a possible detrimental effect of the pathogen, photosynthesis-irradiance response curves and pigment concentrations of healthy and infected plants were compared. Photosynthetic performance of infected plants was significantly reduced and associated with a decrease in chlorophyll a and c content. In Feldmannia irregularis, which had a relatively low photosynthetic capacity, the virus infection produced a more severe effect than in F. simpler.	UNIV LAS PALMAS,INST ALGOL APLICADA,LAS PALMAS,SPAIN; UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY			Sosa, Pedro A./L-1861-2014; ROBLEDO, DANIEL/F-9348-2019; Robledo, Daniel/B-4255-2008	Sosa, Pedro A./0000-0002-8619-3004; ROBLEDO, DANIEL/0000-0003-4931-1937; Robledo, Daniel/0000-0003-4931-1937			DUNCAN MJ, 1982, BOT MAR, V25, P445, DOI 10.1515/botm.1982.25.9.445; DUNSTAN W M, 1973, Journal of Experimental Marine Biology and Ecology, V13, P181, DOI 10.1016/0022-0981(73)90065-8; JEFFREY SW, 1968, BIOL BULL, V135, P149, DOI 10.2307/1539622; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MATTHEWS REF, 1991, PLANT VIROLOGY, P380; MISRA A, 1982, MARINE ALGAE PHARM S, P289; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; RAMUS J, 1981, BIOL SEAWEEDS, P458; Starr R.C., 1987, Journal of Phycology, V23, P1; SUTTLE CA, 1990, NATURE, V347, P467, DOI 10.1038/347467a0; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VANKOOTEN O, 1990, PHYSIOL PLANTARUM, V80, P446, DOI 10.1111/j.1399-3054.1990.tb00066.x	16	10	10	0	3	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	NOV	1994	29	4					247	251		10.1080/09670269400650701			5	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	PR227	WOS:A1994PR22700005		Bronze			2021-04-07	
J	DRING, MJ; FORSTER, RM; SCHMID, R				DRING, MJ; FORSTER, RM; SCHMID, R			ECOLOGICAL SIGNIFICANCE OF BLUE-LIGHT STIMULATION OF PHOTOSYNTHETIC CAPACITY IN LAMINARIA SPP AND OTHER BROWN-ALGAE	MARINE ECOLOGY PROGRESS SERIES			English	Article						BLUE LIGHT; BROWN ALGAE; LAMINARIA; LIGHT QUALITY; PHOTOSYNTHESIS; UNDERWATER LIGHT	FAST RESPONSES; SATURATED PHOTOSYNTHESIS; ECTOCARPUS PHAEOPHYTA; MARINE MACROALGAE; CIRCADIAN-RHYTHM; SURFACE	The transient stimulation of light-saturated photosynthesis in Laminaria digitata (Huds.) Lamour. and L. saccharina (L.) Lamour., which has been observed following pulses of blue light, was found to persist when low irradiances of continuous blue light were given as a supplement to saturating irradiances of red or yellow light. The degree of stimulation was directly proportional to the logarithm of the irradiance of blue light, with a 50% response at 0.28 mumol m-2 s-1 and saturation above 1 mumol m-2 s-1. These irradiances represented about 0.2% and 0.5%, respectively, of the total irradiance incident on the plants. In natural underwater light fields, such low proportions of blue wavelengths would be found only close to, or below, the lower depth limit for Laminaria spp., where photosynthesis, if it occurred at all, would be light-limited and, therefore, not subject to blue light stimulation. Irradiances of blue light measured in the Laminaria zone during periods when the total irradiance was high enough to saturate photosynthesis were always higher than 1 mumol m-2 s-1, and photosynthesis by Laminaria spp. in simulated underwater light fields in the laboratory was not affected by additional blue light. Unlike Laminaria, other brown algae (e.g. Asperococcus, Ectocarpus) exhibited stimulation by blue light in irradiances of red light which are limiting for photosynthesis, and their photosynthetic rates can, therefore, be limited when blue light is present as a higher proportion of the total irradiance than for Laminaria. However, these plants are mostly found in the littoral zone, and will rarely experience low blue light environments. The stimulation of photosynthetic capacity by blue light in brown algae occurs at such low irradiances of blue light that photosynthesis by these plants, in their natural habitats, is unlikely ever to be limited by a shortage of blue light.	QUEENS UNIV BELFAST, SCH BIOL & BIOCHEM, BELFAST BT7 1NN, ANTRIM, NORTH IRELAND			Forster, Rodney/J-1756-2019; Dring, Matthew/B-4941-2014	Forster, Rodney/0000-0001-6990-1673; Dring, Matthew/0000-0001-9043-5670			DRING MJ, 1994, MAR ECOL PROG SER, V104, P131, DOI 10.3354/meps104131; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; Jerlov N.G., 1976, MARINE OPTICS; KAIN JM, 1966, LIGHT ECOLOGICAL FAC, P319; LUNING K, 1980, J PHYCOL, V16, P1; LUNING K, 1985, MAR BIOL, V87, P119, DOI 10.1007/BF00539419; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; SCHMID R, 1994, IN PRESS J PHYCOL	13	5	6	0	10	INTER-RESEARCH	OLDENDORF LUHE	NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY	0171-8630			MAR ECOL PROG SER	Mar. Ecol.-Prog. Ser.	OCT	1994	113	3					271	277		10.3354/meps113271			7	Ecology; Marine & Freshwater Biology; Oceanography	Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography	PR371	WOS:A1994PR37100006		Bronze			2021-04-07	
J	MAIER, I; MULLER, DG; BOLAND, W				MAIER, I; MULLER, DG; BOLAND, W			SPERMATOZOID CHEMOTAXIS IN LAMINARIA-DIGITATA (PHAEOPHYCEAE) .3. PHEROMONE RECEPTOR SPECIFICITY AND THRESHOLD CONCENTRATIONS	ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES			English	Article						LAMINARIA; PHAEOPHYCEAE; PHEROMONE RECEPTOR SPECIFICITY; STRUCTURE-ACTIVITY RELATIONSHIP	ECTOCARPUS-SILICULOSUS; CUTLERIA-MULTIFIDA; SEXUAL PHEROMONES; ALGAE	The pheromone receptor specificity and threshold concentrations have been determined for spermatozoid chemotaxis in Laminaria digitata in structure-activity studies. The results are in agreement with the current concept on the pheromone receptor interaction in brown algae, which is based on mutually-induced dipole-dipole interaction, dispersion forces and hydrophobic forces. Comparison with the receptor specificity in pheromone-induced spermatozoid release in the same species indicates that different pheromone receptors are involved in these two functions.	UNIV BONN,INST ORGAN CHEM & BIOCHEM,D-53121 BONN,GERMANY	MAIER, I (corresponding author), UNIV KONSTANZ,FAK BIOL,POSTFACH 5560,D-78434 CONSTANCE,GERMANY.		Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			BOLAND W, 1982, Z NATURFORSCH C, V37, P5; BOLAND W, 1982, HELV CHIM ACTA, V65, P2355, DOI 10.1002/hlca.19820650742; BOLAND W, 1982, EUR J BIOCHEM, V126, P173, DOI 10.1111/j.1432-1033.1982.tb06763.x; BOLAND W, 1981, Z NATURFORSCH C, V36, P262; BOLAND W, 1984, EUR J BIOCHEM, V144, P169, DOI 10.1111/j.1432-1033.1984.tb08445.x; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLAND W, 1984, HELV CHIM ACTA, V67, P616, DOI 10.1002/hlca.19840670230; CLAYTON MN, 1984, PROGR PHYCOLOGICAL R, V3, P11; MAIER I, 1993, PLANT CELL ENVIRON, V16, P891, DOI 10.1111/j.1365-3040.1993.tb00513.x; MAIER I, 1992, NATURWISSENSCHAFTEN, V79, P420, DOI 10.1007/BF01138576; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MAIER I, 1990, J EXP BOT, V41, P869, DOI 10.1093/jxb/41.7.869; MAIER I, 1984, NATURWISSENSCHAFTEN, V71, P48, DOI 10.1007/BF00365988; MAIER I, 1982, PROTOPLASMA, V113, P137, DOI 10.1007/BF01282003; MAIER I, 1988, NATURWISSENSCHAFTEN, V75, P260, DOI 10.1007/BF00378021; MAIER I, 1987, Z NATURFORSCH C, V42, P948; MARNER FJ, 1984, Z NATURFORSCH C, V39, P689; MULLER DG, 1985, PHYCOLOGIA, V24, P475, DOI 10.2216/i0031-8884-24-4-475.1; MULLER DG, 1976, Z PFLANZENPHYSIOL, V80, P120; SCHROERSISSOKO N, 1988, THESIS U COLOGNO COL	20	12	13	0	14	VERLAG Z NATURFORSCH	TUBINGEN	POSTFACH 2645, W-7400 TUBINGEN, GERMANY	0939-5075			Z NATURFORSCH C	Z.Naturforsch.(C)	SEP-OCT	1994	49	9-10					601	606					6	Biochemistry & Molecular Biology; Pharmacology & Pharmacy	Biochemistry & Molecular Biology; Pharmacology & Pharmacy	PP841	WOS:A1994PP84100010					2021-04-07	
J	SCHMID, R; DRING, MJ; FORSTER, RM				SCHMID, R; DRING, MJ; FORSTER, RM			KINETICS OF BLUE-LIGHT STIMULATION AND CIRCADIAN RHYTHMICITY OF LIGHT-SATURATED PHOTOSYNTHESIS IN BROWN-ALGAE - A SPECIES COMPARISON	JOURNAL OF PHYCOLOGY			English	Article						BLUE LIGHT; CIRCADIAN RHYTHM; PHAEOPHYTA; PHOTOSYNTHESIS; TAXONOMIC DISTRIBUTION	FAST RESPONSES; ECTOCARPUS PHAEOPHYTA; SURFACE	The time courses of photosynthetic rates in red light, with and without additional blue light, were investigated and compared in 20 species of brown algae. Species could be separated into two groups on the basis of the rhythmicity of their photosynthesis in red light and the kinetics of their responses to blue-light pulses. One group, which consisted of members of the Ectocarpales, Chordariales, and Dictyosiphonales, was characterized by strong and persistent circadian rhythmicity in red light. The photosynthetic responses of these species to blue-light pulses started within 10-30 s of the beginning of blue-light treatment and mostly contained at least two distinct kinetic components. An early component, which reached a maximum about 5-10 min after the blue-light pulse, was always detectable. Later components were seen as separate peaks or shoulders after an additional 10-20 min. The decay of the response in this group of species was mostly slow, with half-lives of between 0.5 and 1.5 h. In the second group of species, consisting of members of the Dictyotales, Laminariales, and Fucales, photosynthesis in red light was usually nonrhythmic, although circadian rhythms with a weak amplitude or of transient occurrence were observed in some plants of some species. The increase in photosynthesis in response to a blue-light pulse was not detectable until 70-330 s after the start of blue-light treatment, and the re sponse itself had only a single component, with a maximum after about 10 min and half-life of 10-20 min. The lengths of the lag-phases were positively correlated with the times taken to reach the peak in this group, although the lag-phases and the half-lives sometimes varied with time in individual plants. Two members of the Sphacelariales (Sphacelaria, Cladostephus) did not fit into either of the two groups because their photosynthesis was rhythmic, but their responses had long lag-phases, a single component, and moderately long half-hives. The differences in the kinetics of the photosynthetic response to blue-light pulses, which have been described for the two main groups of species, are thought to indicate that there are two distinct mechanisms by which light-saturated photosynthesis responds to blue light in brown algae. Since in some species the maximal photosynthesis after a blue-light pulse and the rate of photosynthesis in continuous blue light also varied in a circadian pattern, the response to blue light itself may be under circadian control.		SCHMID, R (corresponding author), QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND.		Forster, Rodney/J-1756-2019; Dring, Matthew/B-4941-2014	Forster, Rodney/0000-0001-6990-1673; Dring, Matthew/0000-0001-9043-5670			DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1994, EUR J PHYCOL, V29, P21, DOI 10.1080/09670269400650441; Galland Paul, 1991, P65; HASTINGS JW, 1961, J GEN PHYSIOL, V45, P69, DOI 10.1085/jgp.45.1.69; KAGEYAMA A, 1979, BOT MAR, V22, P199, DOI 10.1515/botm.1979.22.4.199; Provasoli L., 1968, CULTURES COLLECTIONS, P63; SCHMID R, 1993, PLANTA, V191, P489; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; SCHWEIGER HG, 1986, J CELL SCI S, V4, P181; Senger H., 1986, Photomorphogenesis in plants, P137; Sweeney B. M., 1981, INT CELL BIOL 1980 1, P807; SWEENEY BM, 1961, SCIENCE, V134, P1361, DOI 10.1126/science.134.3487.1361; WILKINS MB, 1992, NEW PHYTOL, V121, P347, DOI 10.1111/j.1469-8137.1992.tb02936.x; YAMADA T, 1979, BOT MAR, V22, P203, DOI 10.1515/botm.1979.22.4.203	16	13	14	0	14	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	AUG	1994	30	4					612	621		10.1111/j.0022-3646.1994.00612.x			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	PE430	WOS:A1994PE43000007					2021-04-07	
J	FRIESSKLEBL, AK; KNIPPERS, R; MULLER, DG				FRIESSKLEBL, AK; KNIPPERS, R; MULLER, DG			ISOLATION AND CHARACTERIZATION OF A DNA VIRUS INFECTING FELDMANNIA SIMPLEX (PHAEOPHYCEAE)	JOURNAL OF PHYCOLOGY			English	Article						ALGAL VIRUS; DNA; FELDMANNIA SIMPLEX; MARINE VIRUS; PHAEOPHYCEAE; VIRAL DNA	ALGA ECTOCARPUS-SILICULOSUS; PULSED-FIELD ELECTROPHORESIS; GEL-ELECTROPHORESIS; MARINE; MOLECULES; SEPARATION; GENOME; HOST	We report on the isolation and characterization of a virus that is formed in modified zoidangia of the marine brown alga Feldmannia simplex (Crouan) Hamel (Ectocarpales, Phaeophyceae). Isolated virus particles had a buoyant density of about 1.35 g.mL(-1) in CsCl equilibrium gradients. They contained one major polypeptide (MW = 55,000) and at least six additional polypeptides (MW = 15,000-120,000). Four of these proteins were glycosylated, The viral genome consisted of double-stranded DNA and formed two freely migrating fractions in pulsed-field-gel electrophoresis, namely linear DNA with a size of 220 kilobase pairs, and fragments of 10-60 kilobase pairs. However, electron microscopic examination revealed that a substantial fraction of the viral DNA occurred as closed circles. We suggest that the viral DNA in native particles is circular but tends to break at random sites during the preparation.	UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY							DELIUS H, 1984, J VIROL, V49, P609, DOI 10.1128/JVI.49.2.609-614.1984; DOUCET JP, 1990, ANAL BIOCHEM, V190, P209, DOI 10.1016/0003-2697(90)90182-9; DUBOCHET J, 1982, ADV OPTICAL ELECTRON, V8, P107; FRANCKI RIB, 1991, ARCH VIROL         S, V2, P1; GERSHONI JM, 1985, ANAL BIOCHEM, V146, P59, DOI 10.1016/0003-2697(85)90395-1; HASCHEME.RH, 1970, ADV ENZYMOL REL S BI, V33, P71; HENRY E C, 1991, Journal of Phycology, V27, P30; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; HIGHTOWER RC, 1989, ELECTROPHORESIS, V10, P283, DOI 10.1002/elps.1150100503; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; LEVENE SD, 1987, P NATL ACAD SCI USA, V84, P4054, DOI 10.1073/pnas.84.12.4054; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; OAKLEY BR, 1980, ANAL BIOCHEM, V135, P470; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; Sambrook J, 1989, MOL CLONING LABORATO; SOUTHERN EM, 1987, NUCLEIC ACIDS RES, V15, P5925, DOI 10.1093/nar/15.15.5925; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; STARR RC, 1987, J PHYCOL S2, V123, P1; TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P4350, DOI 10.1073/pnas.76.9.4350; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VOLLENWEIDER HJ, 1975, P NATL ACAD SCI USA, V72, P83, DOI 10.1073/pnas.72.1.83; WATERBURY PG, 1987, NUCLEIC ACIDS RES, V15, P3930, DOI 10.1093/nar/15.9.3930; WESSEL R, 1990, EUR J BIOCHEM, V189, P277, DOI 10.1111/j.1432-1033.1990.tb15487.x; ZIEGLER A, 1987, J CLIN CHEM CLIN BIO, V25, P578	30	19	19	0	5	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	AUG	1994	30	4					653	658		10.1111/j.0022-3646.1994.00653.x			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	PE430	WOS:A1994PE43000013					2021-04-07	
J	MULLER, DG; RAMIREZ, ME				MULLER, DG; RAMIREZ, ME			FILAMENTOUS BROWN-ALGAE FROM THE JUAN-FERNANDEZ ARCHIPELAGO (CHILE) - CONTRIBUTION OF LABORATORY CULTURE TECHNIQUES TO A PHYTOGEOGRAPHIC SURVEY	BOTANICA MARINA			English	Article; Proceedings Paper	Vth International Seaweed Biogeography Workshop	JUL 12-15, 1993	UNIV CAPE TOWN, BOTANY DEPT, CAPE TOWN, SOUTH AFRICA		UNIV CAPE TOWN, BOTANY DEPT		ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; MARINE	The marine benthic floras of many remote localities are poorly studied. Sampling, during a short visit, followed by laboratory culture techniques can be employed to extend the flora lists. Islas Robinson Crusoe and Santa Clara off the coast of central Chile were visited for 10 days in 1991. Small fragments of benthic and sublittoral algae and various substrates were collected and subsequently incubated in the laboratory. The developing ectocarpoid algae were used to establish unialgal cultures. Morphological characters were well expressed, and the following species could be identified and correlated with the taxa reported by Levring in 1941: Ectocarpus fasciculatus, Feldmannia simplex (= Ectocarpus cylindricus Saunders), Hincksia granulosa, H. mitchelliae, H. conifera, (= Ectocarpus arabicus Figari et De Notaris) and Kuetzingiella battersii (= Ectocarpus minutissimus Levring). Feldmannia irregularis, F globifer, Hincksia sandriana, Kuckuckia kylinii and Leptonematella fasciculata are new records for South America. While most of the taxa have wide distributions in cold- and warm-temperate climates, Kuckuckia and Kuetzingiella were previously considered as representatives of the warm temperate North Atlantic flora.	MUSEO NACL HIST NAT,SANTIAGO,CHILE	MULLER, DG (corresponding author), UNIV KONSTANZ,FAK BIOL,UNIV STR 10,D-78434 CONSTANCE,GERMANY.						Abbott I. A., 1976, MARINE ALGAE CALIFOR; ARDRE F, 1969, ACTA BIOLOGICA, V10, P137; ASENSI A O, 1972, Darwiniana (San Isidro), V17, P358; BOLTON JJ, 1987, HELGOLANDER MEERESUN, V41, P165, DOI 10.1007/BF02364698; CARDINAL A, 1964, BEIH NOVA HEDWIGIA, V14, P1; CLAYTON MN, 1964, AUST J BOT, V15, P1; Fletcher RL, 1987, SEAWEEDS BRIT ISLE 1, VIII; JAASUND E., 1976, INTERTIDAL SEAWEEDS; KIM HS, 1992, KOREAN J PHYCOLOGY, V7, P242; KUCKUCK P, 1959, MEERESUNTERS, V5, P292; Levring T., 1941, NAT HIST JUAN FERNANDEZ AND EASTER ISL BOT, V2, P601; Misra J N, 1966, PHAEOPHYCEAE INDIA; Muller D. G., 1991, JPN J PHYCOL, V39, P151; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; PEDERSEN PM, 1989, NORD J BOT, V9, P443, DOI 10.1111/j.1756-1051.1989.tb01024.x; Ramirez M.E., 1991, MONOGR BIOL, V5, P1; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; Santelices B., 1992, Pacific Science, V46, P438; SILVA PC, 1987, SMITHSON CONTRIB MAR, V27, P127; Starr R.C., 1987, Journal of Phycology, V23, P1; Taylor WR, 1960, MARINE ALGAE E TROPI; WOMERSLEY HBS, 1987, MARINE BENTHIC FLORA, V2	25	10	10	0	6	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055			BOT MAR	Bot. Marina	MAY	1994	37	3					205	211		10.1515/botm.1994.37.3.205			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	NQ345	WOS:A1994NQ34500004					2021-04-07	
J	WIENCKE, C; BARTSCH, I; BISCHOFF, B; PETERS, AF; BREEMAN, AM				WIENCKE, C; BARTSCH, I; BISCHOFF, B; PETERS, AF; BREEMAN, AM			TEMPERATURE REQUIREMENTS AND BIOGEOGRAPHY OF ANTARCTIC, ARCTIC AND AMPHIEQUATORIAL SEAWEEDS	BOTANICA MARINA			English	Article; Proceedings Paper	Vth International Seaweed Biogeography Workshop	JUL 12-15, 1993	UNIV CAPE TOWN, BOTANY DEPT, CAPE TOWN, SOUTH AFRICA		UNIV CAPE TOWN, BOTANY DEPT		NORTH-ATLANTIC OCEAN; BENTHIC MARINE-ALGAE; LIFE-HISTORY; SOUTH-AMERICA; DESMARESTIA PHAEOPHYCEAE; LATITUDINAL RANGE; THERMAL ECOTYPES; BROWN-ALGAE; RED ALGAE; GROWTH	The temperature requirements for growth and survival of cold water seaweeds from both Hemispheres are compared and discussed in relation to the climatic history of the various regions and in relation to the origin of amphiequatorial distribution patterns. Endemic Antarctic species are most strongly adapted to low temperatures. In contrast, endemic Arctic macroalgae show higher temperature demands and correspond in their temperature responses to many Antarctic cold-temperate species. Arctic cold-temperate species show similar temperature requirements to cold-temperate species from southernmost South America. The temperature requirements of cold-temperate N. Atlantic species are somewhat higher than those of cold-temperate N. E. Pacific species. These differences are the result of the different times of exposure of these groups to low temperatures. The first steps in the adaptation of macroalgae to low temperatures are an increase in cold tolerance and an increase of growth and reproduction rates at low temperatures. Later, the ability to grow and reproduce at greater-than-or-equal-to 15 to 20-degrees-C and to survive temperatures greater-than-or-equal-to 20-degrees-C is lost. This temperature response type is exemplified in endemic Arctic and Arctic cold-temperate seaweeds exposed to low temperatures since about 3 My. The last steps in the adaptation to low temperatures include the loss of ability to grow and reproduce at greater-than-or-equal-to 5 or 10-degrees-C and a strong reduction in the upper survival temperatures (UST) down to 10-13-degrees-C. This temperature response type is typical for endemic Antarctic species exposed to cold waters for at least 14 My. Amphiequatorial filamentous green and brown algal taxa and microthalli of amphiequatorial brown algae mostly show UST's of 23 to 28.5-degrees-C, significantly higher compared to single Hemisphere taxa from the same regions. These findings strongly favour a migrationist jump across the equator to the other Hemisphere during Pleistocene lowering of the water temperatures in the tropics. Reproduction and growth during the passage across the equator would not have been possible in all species except Ectocarpus siliculosus due to the narrow temperature-reproduction and temperature-growth windows.	BIOL ANSTALT HELGOLAND,D-22607 HAMBURG,GERMANY; INST MEERESKUNDE,MEERESBOT ABT,D-24105 KIEL,GERMANY; UNIV GRONINGEN,DEPT MARINE BIOL,9750 AA HAREN,NETHERLANDS	WIENCKE, C (corresponding author), ALFRED WEGENER INST POLAR & MARINE RES,SEKT BIOL I,COLUMBUSSTR,D-27515 BREMERHAVEN,GERMANY.						ANDERSON RJ, 1989, BOT MAR, V32, P149, DOI 10.1515/botm.1989.32.2.149; BIEBL R, 1939, JB WISS BOT, V88, P389; BISCHOFF B, 1993, HELGOLANDER MEERESUN, V47, P167, DOI 10.1007/BF02430357; BOLTON JJ, 1982, MAR BIOL, V66, P89, DOI 10.1007/BF00397259; BOLTON JJ, 1987, MAR BIOL, V96, P293, DOI 10.1007/BF00427029; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; BRANCH M, 1974, INVEST REP SEA FISH, V104, P1; BREEMAN AM, 1988, HELGOLANDER MEERESUN, V42, P199, DOI 10.1007/BF02366043; BREEMAN AM, 1988, BOT MAR, V37, P171; CAMBRIDGE ML, 1990, BOT MAR, V33, P355, DOI 10.1515/botm.1990.33.4.355; CAMBRIDGE ML, 1987, HELGOLANDER MEERESUN, V41, P329, DOI 10.1007/BF02366197; CLAYTON MN, 1986, BRIT PHYCOL J, V21, P371, DOI 10.1080/00071618600650441; CRAME JA, 1993, TRENDS ECOL EVOL, V8, P162, DOI 10.1016/0169-5347(93)90141-B; DELEPINE R, 1976, B SOC PHYCOL FR, V21, P65; Flohn H., 1984, LATE CAINOZOIC PALAE, P5; FORTES MD, 1980, HELGOLANDER MEERESUN, V34, P15, DOI 10.1007/BF01983538; GUIRY MD, 1987, HELGOLANDER MEERESUN, V41, P283, DOI 10.1007/BF02366193; Heywood R. B., 1984, ANTARCT ECOL, V2, P373; KNOX GA, 1978, 1974 P SCOR SCAR POL, P243; LUNING K, 1984, HELGOLANDER MEERESUN, V38, P305, DOI 10.1007/BF01997486; LUNING K, 1981, BER DEUT BOT GES, V94, P401; LUNING K, 1988, J PHYCOL, V24, P310, DOI 10.1111/j.1529-8817.1988.tb00178.x; Luning K, 1985, MEERESBOTANIK VERBRE; LUNING K, 1989, NATO ASI SERIES G, V22, P187; Luning K, 1990, SEAWEEDS; Nakahara H, 1984, SCI PAP I ALGOL RES, V7, P77; NORTON TA, 1977, NEW PHYTOL, V78, P625, DOI 10.1111/j.1469-8137.1977.tb02167.x; NOVACZEK I, 1986, CAN J BOT, V64, P2414, DOI 10.1139/b86-321; NOVACZEK I, 1984, MAR BIOL, V82, P241, DOI 10.1007/BF00392405; NOVACZEK I, 1990, HELGOLANDER MEERESUN, V44, P475, DOI 10.1007/BF02365481; NOVACZEK I, 1990, HELGOLANDER MEERESUN, V44, P459, DOI 10.1007/BF02365480; OHNO M, 1984, MEM NATN I POLAR RES, V32, P112; PETERS AF, 1991, REV CHIL HIST NAT, V64, P261; PETERS AF, 1992, BRIT PHYCOL J, V27, P177, DOI 10.1080/00071619200650181; PETERS AF, 1986, CAN J BOT, V64, P2192, DOI 10.1139/b86-291; PETERS AF, 1993, MAR BIOL, V115, P143, DOI 10.1007/BF00349396; PETERS AF, 1992, J PHYCOL, V28, P428, DOI 10.1111/j.0022-3646.1992.00428.x; RAMIREZ ME, 1986, CAN J BOT, V64, P2948, DOI 10.1139/b86-389; RUENESS J, 1984, HYDROBIOLOGIA, V116, P303, DOI 10.1007/BF00027690; SKOTTSBERG CJF, 1964, 1962 S BIOL ANT PAR, P147; Sokal R. R, 1973, INTRO BIOSTATISTICS; STACHE B, 1993, THESIS HARTUNG GORRE; STAM WT, 1988, HELGOLANDER MEERESUN, V42, P251, DOI 10.1007/BF02366045; TOMDIECK I, 1993, MAR BIOL, V115, P151; TOMDIECK I, 1992, MAR ECOL-PROG SER, V31, P147; VANDENHOEK C, 1982, HELGOLANDER MEERESUN, V35, P153; VANDENHOEK C, 1982, BIOL J LINN SOC, V18, P81; VANDENHOEK C, 1990, EVOLUTIONARY BIOGEOG, P55; VANDENHOEK C, 1989, NATO ASI SERIES G, V22, P57; VANOPPEN MJH, 1993, MAR BIOL, V115, P381, DOI 10.1007/BF00349835; VANOPPEN MJH, IN PRESS J PHYCOL; WIENCKE C, 1990, MAR ECOL PROG SER, V59, P157, DOI 10.3354/meps059157; WIENCKE C, 1993, BOT ACTA, V106, P78, DOI 10.1111/j.1438-8677.1993.tb00341.x; WIENCKE C, 1990, MAR ECOL PROG SER, V65, P283, DOI 10.3354/meps065283; WIENCKE C, 1989, MAR ECOL PROG SER, V54, P189, DOI 10.3354/meps054189; YARISH C, 1986, BOT MAR, V29, P215, DOI 10.1515/botm.1986.29.3.215; YARISH C, 1987, HELGOLANDER MEERESUN, V41, P323, DOI 10.1007/BF02366196; YARISH C, 1984, HELGOLANDER MEERESUN, V38, P273, DOI 10.1007/BF01997485	58	77	84	2	25	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055			BOT MAR	Bot. Marina	MAY	1994	37	3					247	259		10.1515/botm.1994.37.3.247			13	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	NQ345	WOS:A1994NQ34500009					2021-04-07	
J	LUNING, K				LUNING, K			WHEN DO ALGAE GROW - THE 3RD FOUNDERS LECTURE	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article							CIRCADIAN-RHYTHMS; SEASONAL GROWTH; PTERYGOPHORA-CALIFORNICA; LAMINARIA-LONGICRURIS; ECTOCARPUS PHAEOPHYTA; FAST RESPONSES; BROWN ALGA; BLUE-LIGHT; PHOTOSYNTHESIS; SYNCHRONIZATION			LUNING, K (corresponding author), BIOL ANSTALT HELGOLAND,NOTKESTR 31,D-22607 HAMBURG,GERMANY.						ANDERSON DM, 1987, NATURE, V325, P616, DOI 10.1038/325616a0; BRITZ SJ, 1976, PLANT PHYSIOL, V58, P22, DOI 10.1104/pp.58.1.22; BRITZ SJ, 1983, EUR J CELL BIOL, V31, P1; BUGGELN RG, 1978, J PHYCOL, V14, P156, DOI 10.1111/j.1529-8817.1978.tb02441.x; Bunning E, 1964, HDB BEHAVIOURAL NEUR; CHAPMAN ARO, 1977, MAR BIOL, V40, P197, DOI 10.1007/BF00390875; CONNOLLY NJ, 1985, S Z N I MAR ECOL, V6, P181; CONOLLY NJ, 1985, PSZNI MAR ECOL, V6, P299; COSTAS E, 1991, PHYCOLOGIA, V30, P597, DOI 10.2216/i0031-8884-30-6-597.1; DIECK IT, 1991, J PHYCOL, V27, P341, DOI 10.1111/j.0022-3646.1991.00341.x; Dring M. J., 1984, PROGR PHYCOLOGICAL R, V3, P159; Dring M. J., 1971, 4TH EUR MAR BIOL S C, P375; DRUEHL LD, 1987, CAN J BOT, V65, P1599, DOI 10.1139/b87-219; Edmunds LN, 1988, CELLULAR MOL BASES B; FALLIS AL, 1916, PUGET SOUND BIOL STA, V1, P137; FOGG GE, 1990, BRIT PHYCOL J, V25, P103, DOI 10.1080/00071619000650101; GAGNE JA, 1982, MAR BIOL, V69, P91, DOI 10.1007/BF00396965; GESSNER F, 1957, MEER STRAND; GWINNER E, 1981, NATURWISSENSCHAFTEN, V68, P542, DOI 10.1007/BF00401662; Gwinner E., 1986, CIRCANNUAL RHYTHMS E; HASTINGS JW, 1958, BIOL BULL, V115, P440, DOI 10.2307/1539108; Hastings JW, 1991, NEURAL INTEGRATIVE A, P435; KAGEYAMA A, 1979, BOT MAR, V22, P199, DOI 10.1515/botm.1979.22.4.199; KAIN JM, 1989, BRIT PHYCOL J, V24, P203, DOI 10.1080/00071618900650221; KAIN JM, 1963, J MAR BIOL ASSOC UK, V43, P129, DOI 10.1017/S0025315400005312; KAIN JM, 1987, PHYCOLOGIA, V26, P88, DOI 10.2216/i0031-8884-26-1-88.1; KAIN JM, 1962, J MAR BIOL ASSOC UK, V42, P377, DOI 10.1017/S0025315400001363; KAIN JM, 1960, J MAR BIOL ASSOC UK, V39, P609, DOI 10.1017/S0025315400013588; KAIN JM, 1987, J PHYCOL, V23, P464, DOI 10.1111/j.1529-8817.1987.tb02533.x; LINZELL JL, 1973, J PHYSIOL-LONDON, V230, P225, DOI 10.1113/jphysiol.1973.sp010185; LUNING K, 1979, MAR ECOL PROG SER, V1, P195, DOI 10.3354/meps001195; LUNING K, 1993, PHYCOLOGIA, V32, P379, DOI 10.2216/i0031-8884-32-5-379.1; LUNING K, 1986, BRIT PHYCOL J, V21, P269; LUNING K, 1991, BOT ACTA, V104, P157; LUNING K, 1988, MAR ECOL PROG SER, V45, P137, DOI 10.3354/meps045137; LUNING K, 1992, J PHYCOL, V28, P794, DOI 10.1111/j.0022-3646.1992.00794.x; LUNING K, 1973, MAR BIOL, V23, P275, DOI 10.1007/BF00389334; LUNING K, 1994, IN PRESS PHYCOLOGIA, V33; LUNING K, 1994, IN PRESS J PHYCOL, V30; LUNING K, 1993, HYDROBIOLOGIA, V260, P1; MOLLENHAUER D, 1988, HELGOLANDER MEERESUN, V42, P385, DOI 10.1007/BF02365618; NIENBURG W, 1925, WISS MEERESUNTERS H, V15, P1; NORTON TA, 1987, BRIT PHYCOL J, V22, P317, DOI 10.1080/00071618700650361; NULTSCH W, 1984, MAR BIOL, V81, P217, DOI 10.1007/BF00393215; OOHUSA T, 1980, BOT MAR, V23, P1, DOI 10.1515/botm.1980.23.1.1; OSTGAARD K, 1982, MAR BIOL, V66, P261, DOI 10.1007/BF00397031; PARKE M, 1948, J MAR BIOL ASSOC UK, V27, P651, DOI 10.1017/S0025315400056071; PENGELLEY ET, 1976, COMP BIOCH PHYSL A, V54, P273; PENGELLEY ET, 1974, CIRCANNUAL CLOCKS; PRINTZ H, 1926, SKR NORSKE VIDENS MN, V5, P1; SCHAFFELKE B, 1994, EUR J PHYCOL, V29, P49, DOI 10.1080/09670269400650471; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; STRASBURGER E, 1904, STREIFZUGE RIVIERA; STROMGREN T, 1977, J EXP MAR BIOL ECOL, V29, P181, DOI 10.1016/0022-0981(77)90047-8; STROMGREN T, 1986, MAR BIOL, V90, P467, DOI 10.1007/BF00428570; STROMGREN T, 1978, SARSIA, V63, P155; Sweeney B.M., 1983, Progress phycol. Res., V2, P189; SWEENEY BM, 1987, RHYTHMIC PHENOMENA P; Sweeney BM, 1969, RHYTHMIC PHENOMENA P; TYLER PA, 1992, BRIT PHYCOL J, V27, P353, DOI 10.1080/00071619200650301; WAALAND SD, 1972, PLANTA, V105, P196, DOI 10.1007/BF00385391	62	5	6	0	2	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	MAY	1994	29	2					61	67		10.1080/09670269400650501			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	NR793	WOS:A1994NR79300001		Bronze			2021-04-07	
J	PARODI, ER; MULLER, DG				PARODI, ER; MULLER, DG			FIELD AND CULTURE STUDIES ON VIRUS-INFECTIONS IN HINCKSIA-HINCKSIAE AND ECTOCARPUS-FASCICULATUS (ECTOCARPALES, PHAEOPHYCEAE)	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						ECTOCARPALES; ECTOCARPUS; HINCKSIA; MARINE VIRUS; PHAEOPHYCEAE	DNA VIRUS; MARINE; SILICULOSUS	Dense populations of Hincksia hincksiae and Ectocarpus fasiculatus were found on blades of Laminaria hyperborea and Saccorhiza polyschides in Brittany, France. Clonal cultures of healthy and infected plants were established, and infectivity of the virus particles was demonstrated. Size and morphology of the virions are similar to those previously found in Ectocarpus siliculosus and in the genus Feldmannia.	UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY	PARODI, ER (corresponding author), UNIV NACL SUR,DEPT BIOL,RA-8000 BAHIA BLANCA,ARGENTINA.						BERGH O, 1989, NATURE, V340, P467, DOI 10.1038/340467a0; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; CLITHEROE SB, 1974, J ULTRA MOL STRUCT R, V49, P211, DOI 10.1016/S0022-5320(74)80032-8; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1993, PROTOPLASMA, V175, P121, DOI 10.1007/BF01385009; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; Muller DG, 1993, HYDROBIOLOGIA, V260/261, P37; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; SAUVAGEAU C, 1987, J BOTANIQUE, V11, P66; Starr R.C., 1987, Journal of Phycology, V23, P1; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	15	17	17	0	6	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	MAY	1994	29	2					113	117		10.1080/09670269400650561			5	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	NR793	WOS:A1994NR79300007		Bronze			2021-04-07	
J	SCHMID, CE; MULLER, DG; EICHENBERGER, W				SCHMID, CE; MULLER, DG; EICHENBERGER, W			ISOLATION AND CHARACTERIZATION OF A NEW PHOSPHOLIPID FROM BROWN-ALGAE - INTRACELLULAR-LOCALIZATION AND SITE OF BIOSYNTHESIS	JOURNAL OF PLANT PHYSIOLOGY			English	Article						ECTOCARPUS SILICULOSUS; LIPID PATTERN; LONG-CHAIN POLYUNSATURATED FATTY ACIDS; NMR; PHAEOPHYCEAE; PHEROMONE PRECURSOR; SEX SPECIFICITY	ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; LIPID-METABOLISM; FATTY-ACIDS; MEMBRANES	In various orders of brown algae a novel lipid constituent PX was detected and suggested to be a phosphoglyceride bearing an amino-containing polar head group. We discuss its tentative structure based on IR, NMR, MS and chemical data. PX accounted for 2-4% of total lipids and always exhibited a fatty acid composition rich in 20:4 (arachidonic) and 20:5 (eicosapentaenoic) acids. High proportions of these long-chain polyunsaturated acids implied a predominantly cytoplasmic biosynthesis of its diglyceride part. In Ectocarpus siliculosus (Phaeophyceae) PE has a fatty acid pattern similar to PX. In its gametes PX accumulates in the plasma membrane and, in addition, shows a characteristic sex-specific composition of its acyl moieties. In female plasma membranes the content of 20:5 acid is significantly higher than in male plasma membranes or whole plants. This is possibly due to 20:5 acid from PX acting as a direct precursor for pheromone biosynthesis. PX is suggested to be of special physiological importance in terms of an acyl donor in this process. The 20:5 pool in PX was estimated to be sufficient to synthesize 70% of the pheromone ectocarpene.	UNIV KONSTANZ, FAK BIOL, D-78434 CONSTANCE, GERMANY	SCHMID, CE (corresponding author), UNIV BERN, INST BIOCHEM, CH-3012 BERN, SWITZERLAND.						ARAKI S, 1991, PLANT CELL PHYSIOL, V32, P623, DOI 10.1093/oxfordjournals.pcp.a078124; CHAPMAN DJ, 1987, METHOD ENZYMOL, V148, P294; CHEN PS, 1956, ANAL CHEM, V28, P1756, DOI 10.1021/ac60119a033; COHEN Z, 1992, PLANT PHYSIOL, V98, P569, DOI 10.1104/pp.98.2.569; DEMBITSKY VM, 1990, PHYTOCHEMISTRY, V29, P3417, DOI 10.1016/0031-9422(90)85249-F; DITTMER JC, 1964, J LIPID RES, V5, P126; FAHMY AR, 1961, HELV CHIM ACTA, V44, P2022, DOI 10.1002/hlca.19610440727; HARWOOD JL, 1989, ADV BOT RES, V16, P1; HARWOOD JL, 1987, METHOD ENZYMOL, V148, P475; HEINZ E, 1967, BIOCHIM BIOPHYS ACTA, V144, P333, DOI 10.1016/0005-2760(67)90162-2; HOFMANN M, 1993, THESIS U BERN; KLENK E, 1963, H-S Z PHYSIOL CHEM, V334, P44, DOI 10.1515/bchm2.1963.334.1.44; Larsson C., 1990, The plant plasma membrane., P1; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; Pohl P., 1979, MARINE ALGAE PHARM S, P473; SCHMID CE, 1992, PLANT PHYSIOL BIOCH, V30, P703; SCHMID CE, 1991, BIOL CHEM H-S, V372, P540; SMITH KL, 1984, J EXP BOT, V35, P1359, DOI 10.1093/jxb/35.9.1359; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; STRATMANN K, 1992, ANGEW CHEM, V104, P1261; THIES W, 1971, Z PFLANZENZUCHTUNG, V65, P181; VANDENHOEK C, 1968, BLUMEA, V16, P193	22	12	12	0	4	ELSEVIER GMBH, URBAN & FISCHER VERLAG	JENA	OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY	0176-1617	1618-1328		J PLANT PHYSIOL	J. Plant Physiol.	MAY	1994	143	4-5					570	574		10.1016/S0176-1617(11)81826-2			5	Plant Sciences	Plant Sciences	NN324	WOS:A1994NN32400029					2021-04-07	
J	HENRY, EC; MEINTS, RH				HENRY, EC; MEINTS, RH			RECOMBINANT VIRUSES AS TRANSFORMATION VECTORS OF MARINE MACROALGAE	JOURNAL OF APPLIED PHYCOLOGY			English	Article; Proceedings Paper	Colloquium on Microalgal Biotechnology and Commercial Applications, at the 47th Annual Meeting of the Phycological-Society-of-America	AUG 03-04, 1993	IOWA STATE UNIV, AMES, IA	PHYCOL SOC AMER INC	IOWA STATE UNIV	ALGAE; GENES; RECOMBINANT; TRANSFORMATION; VECTORS; VIRUSES	ALGA ECTOCARPUS-SILICULOSUS; EUKARYOTIC GREEN-ALGA; URONEMA-GIGAS; PHAEOPHYCEAE; INFECTION; PARTICLES	The large dsDNA viruses that are known to infect eukaryotic algae show promise as genetic vectors for algal biotechnology. The large size (150-330 kbp) of these viral genomes may permit insertion of large sequences of foreign DNA. The viruses infecting filamentous marine brown algae appear to be integrated into the genomes of their hosts, and may provide integration mechanisms that can be used for directing insertion of foreign genes into algal chromosomes.	OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,CORVALLIS,OR 97333; OREGON STATE UNIV,CTR GENE RES & BIOTECHNOL,CORVALLIS,OR 97333							BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; Cavalier-Smith T., 1986, Progress phycol. Res., V4, P309; Chapman VJ, 1980, SEAWEEDS THEIR USES; CLITHEROE SB, 1974, J ULTRA MOL STRUCT R, V49, P211, DOI 10.1016/S0022-5320(74)80032-8; COLE A, 1980, VIROLOGY, V100, P166, DOI 10.1016/0042-6822(80)90562-0; DODDS JA, 1980, VIROLOGY, V100, P156; EMERSON CJ, 1982, CAN J BOT, V60, P2164, DOI 10.1139/b82-266; GIBBS A, 1975, VIROLOGY, V64, P571, DOI 10.1016/0042-6822(75)90136-1; HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; HRUBY DE, 1990, CLIN MICROBIOL REV, V3, P153, DOI 10.1128/CMR.3.2.153-170.1990; KUCKKUCK P, 1894, WISS MEERESUNTERSUCH, V1, P220; LACLAIRE JW, 1977, PROTOPLASMA, V93, P127, DOI 10.1007/BF01276287; MARKEY D R, 1974, Protoplasma, V80, P223, DOI 10.1007/BF01666361; MARTIN EL, 1982, SELECTED PAPERS PHYC, V2, P793; MOE RL, 1981, J PHYCOL, V17, P15, DOI 10.1111/j.0022-3646.1981.00015.x; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1972, SOC BOT FR MEM, P87; MULLER DG, 1972, PHYCOLOGIA, V11, P11; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; ROSEVINGE LK, 1893, MEDDELELSER GRONLAND, V3, P7665; SAFFERMAN RS, 1991, EPA600491023 OFF RES; Saunders D.A, 1898, P CALIF ACAD SCI 3, V1, P147; SAUVAGEAU C, 1896, J BOTANIQUE, V10, P140; SCHMITZ K, 1976, MAR BIOL, V36, P207, DOI 10.1007/BF00389281; Setchell WA, 1925, U CALIF PUBL BOT, V8, P383; Sherman LA, 1978, COMPREHENSIVE VIROLO, P145; SKOTNICKI A, 1976, VIROLOGY, V75, P457, DOI 10.1016/0042-6822(76)90043-X; Smith GM, 1942, AM J BOT, V29, P645, DOI 10.2307/2437177; STANKER LH, 1980, VIROLOGY, V114, P357; TOTH R, 1972, J PHYCOL, V8, P126, DOI 10.1111/j.1529-8817.1972.tb04011.x; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; YOUNG R, 1983, SCIENCE, V222, P777; YOUNG RA, 1983, P NATL ACAD SCI-BIOL, V80, P1194, DOI 10.1073/pnas.80.5.1194	36	17	18	0	7	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0921-8971			J APPL PHYCOL	J. Appl. Phycol.	APR	1994	6	2					247	253		10.1007/BF02186078			7	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	NJ473	WOS:A1994NJ47300018					2021-04-07	
J	LUNING, K				LUNING, K			CIRCADIAN GROWTH RHYTHM IN JUVENILE SPOROPHYTES OF LAMINARIALES (PHAEOPHYTA)	JOURNAL OF PHYCOLOGY			English	Article						CIRCADIAN RHYTHM; GROWTH RATE; LAMINARIALES; PHAEOPHYTA; PTERYGOPHORA-CALIFORNICA	LIGHT-SATURATED PHOTOSYNTHESIS; BLUE-LIGHT; ECTOCARPUS PHAEOPHYTA; EXTERNAL FACTORS; FAST RESPONSES; CELL-DIVISION; ALGAE	A circadian rhythm in growth was detected by computer-aided image analysis in 3-4-cm-long, juvenile sporophytes of the kelp species Pterygophora california Rupr. and in seven Laminaria spp. In P. californica, the free-running rhythm occurred in continuous white fluorescent light, had a period of 26 h at 10-degrees or 15-degrees-C, and persisted for at least 2 weeks in white or blue light. The rhythm became insignificant in continuous green or red light after 3 cycles. Synchronization by white light-dark regimes, e.g. by 16 h light per day, resulted in an entrained period of 24 h and in a shift of the circadian growth minimum into the middle of the light phase. A morning growth peak represented the decreasing portion of the circadian growth curve, and an evening peak the increasing portion. The circadian growth peak was not visible during the dark phase, because growth rate decreased immediately after the onset of darkness. At night, some growth still occurred at 16 or 12 h light per day, whereas growth stopped completely at 8 h light per day, as in continuous darkness. During 11 days of darkness, the thallus area became reduced by 3.5%, but growth rate recovered in subsequent light-dark cycles, and the circadian growth rhythm reappeared in subsequent continuous light.		LUNING, K (corresponding author), BIOL ANSTALT HELGOLAND,NOTKESTR 31,D-22607 HAMBURG 52,GERMANY.						ASCHOFF J, 1979, Z TIERPSYCHOL, V49, P225; ASCHOFF J, 1981, HDB BEHAVIORAL NEURO, V4; BRUCE VG, 1970, J PROTOZOOL, V17, P328, DOI 10.1111/j.1550-7408.1970.tb02380.x; Bunning E., 1973, PHYSL CLOCK; CHISHOLM SW, 1981, J EXP MAR BIOL ECOL, V51, P107, DOI 10.1016/0022-0981(81)90123-4; Chisholm SW, 1981, CAN B FISH AQUAT SCI, V210, P150; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; Edmunds Jr L. N., 1988, CELL CYCLE CLOCKS; Edmunds L. N., 1984, CELL CYCLE CLOCKS; EDMUNDS LN, 1966, J CELL PHYSIOL, V67, P35, DOI 10.1002/jcp.1040670105; Evans GC, 1972, QUANTITATIVE ANAL PL; FELDMAN JF, 1990, SCI PHOTOBIOLOGY, P193; FORSTER R M, 1990, British Phycological Journal, V25, P87; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; HADER DP, 1988, P INDIAN AS-PLANT SC, V98, P227; HADER DP, 1988, J PHOTOCH PHOTOBIO B, V1, P385, DOI 10.1016/1011-1344(88)85001-2; KAIN JM, 1987, PHYCOLOGIA, V26, P88, DOI 10.2216/i0031-8884-26-1-88.1; KIRST GO, 1990, ANNU REV PLANT PHYS, V41, P21, DOI 10.1146/annurev.pp.41.060190.000321; L?ning K., 1990, SEAWEEDS THEIR ENV B; LUNING K, 1975, MAR BIOL, V29, P195, DOI 10.1007/BF00391846; LUNING K, 1992, J PHYCOL, V28, P794, DOI 10.1111/j.0022-3646.1992.00794.x; LUNING K, 1981, BR J PHYCOL, V16, P579; Moore-Ede M.C., 1982, CLOCKS TIME US; MULLER S, 1976, Z PFLANZENPHYSIOL, V78, P461; NEUSCHELERWIRTH H, 1970, Z PFLANZENPHYSIOL, V63, P352; OOHUSA T, 1980, BOT MAR, V23, P1, DOI 10.1515/botm.1980.23.1.1; OSTGAARD K, 1982, MAR BIOL, V66, P261, DOI 10.1007/BF00397031; PRINGLE J D, 1970, Journal of Experimental Marine Biology and Ecology, V5, P113, DOI 10.1016/0022-0981(70)90012-2; Pringle J. D., 1969, P INT SEAWEED S, V6, P41; SCHMID R, 1986, J INTERDISCIPL CYCLE, V17, P99; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1987, BLUE LIGHT RESPONSES, V2, P87; Starr R.C., 1987, Journal of Phycology, V23, P1; STROMGREN T, 1978, SARSIA, V63, P155; Sweeney B.M., 1983, Progress phycol. Res., V2, P189; SWEENEY BM, 1982, PLANT PHYSIOL, V70, P272, DOI 10.1104/pp.70.1.272; SWEENEY BM, 1958, J PROTOZOOL, V5, P217, DOI 10.1111/j.1550-7408.1958.tb02555.x; SWEENEY BM, 1960, COLD SPRING HARB SYM, V25, P87, DOI 10.1101/SQB.1960.025.01.009; SWEENEY BM, 1987, RHYTHMIC PHENOMENA P; VOGEL K, 1990, 4TH P EUR S LIF SCI, P541; WAALAND SD, 1972, PLANTA, V105, P196, DOI 10.1007/BF00385391	42	17	18	0	4	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	APR	1994	30	2					193	199		10.1111/j.0022-3646.1994.00193.x			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	NK490	WOS:A1994NK49000004					2021-04-07	
J	FORSTER, RM; DRING, MJ				FORSTER, RM; DRING, MJ			INFLUENCE OF BLUE-LIGHT ON THE PHOTOSYNTHETIC CAPACITY OF MARINE PLANTS FROM DIFFERENT TAXONOMIC, ECOLOGICAL AND MORPHOLOGICAL GROUPS	EUROPEAN JOURNAL OF PHYCOLOGY			English	Article						BLUE LIGHT; BROWN ALGAE; LITTORAL ECOLOGY; MORPHOLOGY; PHOTOSYNTHESIS	INORGANIC-CARBON; SATURATED PHOTOSYNTHESIS; ECTOCARPUS PHAEOPHYTA; CIRCADIAN-RHYTHM; FAST RESPONSES; MACROALGAE; ASSIMILATION; ALGAE	The photosynthetic rates of 47 species of marine plants (mainly seaweeds) have been measured in a saturating irradiance of red light before and after exposure to a 2 min pulse of blue light. In the majority of brown algae tested (20 out of 25 species) the pulse of blue light caused a rapid and significant increase in the rate of photosynthesis, often of over 100%, which decayed away over the following 1-3 h. However, apart from a small response in Codium, blue light did not affect the light-saturated photosynthesis in any species of green or red algae tested, or in a planktonic diatom (Phaeodactylum tricornutum) or a seagrass (Zostera noltii). This type of response to blue light is, therefore, common among species of brown macroalgae but appears to be absent from all other taxonomic groups of marine plants tested so far. within the brown algae, the greatest response to blue light was recorded in species from the littoral zone, and little or no stimulation was observed in sublittoral species. Brown algae with a thin or filamentous morphology exhibited a higher degree of blue light stimulation than species with thicker thalli. The maximum photosynthetic rate in red light of thin, littoral species such as Petalonia fascia or Colpomenia peregrina could be transiently increased by over 200% after exposure to blue light at 50 mumol m-2 s-1 for 2 min. The size of this contribution to the photosynthetic performance of the plant suggests that blue wavelengths are essential for maximal productivity of these species in the natural environment. Members of the Fucaceae were the only littoral brown algae which showed little or no photosynthetic response to pulses of blue light. Previous work had indicated that the stimulatory effect of blue light was the result of an increase in the rate of transport of inorganic carbon from seawater to the chloroplast. The distribution of the response among the brown algae supports this hypothesis, since blue light stimulation was more prevalent in algae from habitats in which inorganic carbon frequently limits photosynthesis (e.g. high light and low water movement in the littoral environment) than in those from habitats where plant growth is primarily limited by the availability of light (e.g. the sublittoral zone).	QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND			Dring, Matthew/B-4941-2014; Forster, Rodney/J-1756-2019	Dring, Matthew/0000-0001-9043-5670; Forster, Rodney/0000-0001-6990-1673			AXELSSON L, 1988, MAR BIOL, V97, P295, DOI 10.1007/BF00391315; BJORK M, 1992, PLANTA, V187, P152, DOI 10.1007/BF00201637; Drew EA, 1983, PSZNI MAR ECOL, V4, P211, DOI DOI 10.1111/J.1439-0485.1983.TB00297.X; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; HATCHER BG, 1977, MAR BIOL, V43, P381, DOI 10.1007/BF00396932; LARSSON C, 1990, CURRENT RES PHOTOSYN, V4, P533; LITTLER MM, 1982, J PHYCOL, V18, P307, DOI 10.1111/j.1529-8817.1982.tb03188.x; MABERLY SC, 1990, J PHYCOL, V26, P439, DOI 10.1111/j.0022-3646.1990.00439.x; MADSEN TV, 1991, AQUAT BOT, V41, P5, DOI 10.1016/0304-3770(91)90037-6; RAVEN JA, 1991, CAN J BOT, V69, P908, DOI 10.1139/b91-118; RAVEN JA, 1992, FUNCT ECOL, V6, P41, DOI 10.2307/2389769; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; South G. R., 1986, CHECKLIST DISTRIBUTI; SURIF MB, 1989, OECOLOGIA, V78, P97, DOI 10.1007/BF00377203	16	29	30	0	37	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0967-0262			EUR J PHYCOL	Eur. J. Phycol.	FEB	1994	29	1					21	27		10.1080/09670269400650441			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	NF927	WOS:A1994NF92700004					2021-04-07	
J	SCHMID, CE; SCHROER, N; MULLER, DG				SCHMID, CE; SCHROER, N; MULLER, DG			FEMALE GAMETE MEMBRANE-GLYCOPROTEINS POTENTIALLY INVOLVED IN GAMETE RECOGNITION IN ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	PLANT SCIENCE			English	Article						CELL-CELL RECOGNITION; ECTOCARPUS; FERTILIZATION; LECTIN WGA; N-ACETYLGLUCOSAMINE; SEX-SPECIFIC PROTEIN PATTERNS	CHLAMYDOMONAS-EUGAMETOS; GENETIC-VARIABILITY; MOUSE ORGANS; PROTEINS; ALGA; POLYACRYLAMIDE; AGGLUTININS; LECTIN; SPERM; GELS	In the plasma membrane-enriched fraction of female gametes of Ectocarpus siliculosus (Phaeophyceae) four sex-specific glycoproteins (P2-3, P5, P16) exposing N-acetylglucosamine (GlcNAc) residues were detected in vitro. In immunoblots these proteins were labelled with the GlcNAc complementary lectin wheat germ agglutinin (WGA) conjugated to digoxigenin. P2 also cross-reacted with the mannose-specific Galanthus nivalis agglutinin (GNA). Previously, a key and lock mechanism of gamete recognition was demonstrated in vivo: fertilization can be selectively inhibited by a WGA pretreatment of female gametes, or by GlcNAc pretreated male gametes. Lectins with different carbohydrate requirements such as GNA do not affect fertilization in vivo. Thus, the remaining glycoproteins P3, P5 and P16 seem to be involved in cell-cell recognition during fertilization, and possibly one of them functions as the male receptor in the plasma membrane of female gametes. Membrane proteins of both gamete types showed higher differences than cytosolic proteins or proteins extracted from whole cells when examined by high resolution two-dimensional gel electrophoresis.		SCHMID, CE (corresponding author), UNIV KONSTANZ,FAK BIOL,POSTFACH 5560,D-78434 CONSTANCE,GERMANY.						AQUADRO CF, 1981, P NATL ACAD SCI-BIOL, V78, P3784, DOI 10.1073/pnas.78.6.3784; BIRD KT, 1993, J APPL PHYCOL, V5, P213, DOI 10.1007/BF00004020; BRAWLEY SH, 1991, DEV BIOL, V144, P94, DOI 10.1016/0012-1606(91)90482-I; CROWLEY JF, 1984, ARCH BIOCHEM BIOPHYS, V231, P524, DOI 10.1016/0003-9861(84)90417-X; DIETL J, 1987, NATURWISSENSCHAFTEN, V74, P235, DOI 10.1007/BF00424593; GELLER A, 1981, J EXP BIOL, V92, P53; Goldstein I. J., 1986, LECTINS PROPERTIES F, P33, DOI DOI 10.1016/B978-0-12-449945-4.50007-5; HASELBECK A, 1990, ANAL BIOCHEM, V191, P25, DOI 10.1016/0003-2697(90)90381-I; HEUKESHOVEN J, 1985, ELECTROPHORESIS, V6, P103, DOI 10.1002/elps.1150060302; JUNGBLUT P, 1985, BIOCHEM GENET, V23, P227, DOI 10.1007/BF00504321; KIM GH, 1993, PROTOPLASMA, V174, P69, DOI 10.1007/BF01404044; KLOSE J, 1981, BIOCHEM GENET, V19, P859, DOI 10.1007/BF00504251; KOOIJMAN R, 1990, PLANTA, V181, P529, DOI 10.1007/BF00193006; KOOIJMAN R, 1989, J CELL BIOL, V109, P1677, DOI 10.1083/jcb.109.4.1677; KYHSEANDERSEN J, 1984, J BIOCHEM BIOPH METH, V10, P203, DOI 10.1016/0165-022X(84)90040-X; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; PETERSON GL, 1977, ANAL BIOCHEM, V83, P346, DOI 10.1016/0003-2697(77)90043-4; PIERCE M, 1983, J BIOL CHEM, V258, P3576; ROGERS DJ, 1991, LECTIN REV, V1, P129; ROSIERE TK, 1992, DEV BIOL, V154, P309, DOI 10.1016/0012-1606(92)90070-W; SCHMID CE, 1992, PLANT PHYSIOL BIOCH, V30, P703; SCHMID CE, 1994, J PLANT PHYSIOL, V143, P570, DOI 10.1016/S0176-1617(11)81826-2; Schmid Christine E., 1993, Hydrobiologia, V260-261, P437, DOI 10.1007/BF00049053; SHIBUYA N, 1988, J BIOL CHEM, V263, P728; STRATMANN K, 1993, TETRAHEDRON, V49, P3755, DOI 10.1016/S0040-4020(01)90228-5; TRIMMER JS, 1985, CELL, V40, P697, DOI 10.1016/0092-8674(85)90218-1; VERSLUIS M, 1993, J GEN MICROBIOL, V139, P763, DOI 10.1099/00221287-139-4-763; VIERBUCHEN M, 1991, CELL RECEPTORS, P272	32	13	13	0	8	ELSEVIER SCI IRELAND LTD	CLARE	CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND	0168-9452			PLANT SCI	Plant Sci.		1994	102	1					61	67		10.1016/0168-9452(94)90021-3			7	Biochemistry & Molecular Biology; Plant Sciences	Biochemistry & Molecular Biology; Plant Sciences	PL258	WOS:A1994PL25800007					2021-04-07	
J	FEHRMANN, C; POHL, P				FEHRMANN, C; POHL, P			CADMIUM ADSORPTION BY THE NONLIVING BIOMASS OF MICROALGAE GROWN IN AXENIC MASS-CULTURE	JOURNAL OF APPLIED PHYCOLOGY			English	Article						CADMIUM ADSORPTION; MICROALGAE; CYANOBACTERIA; NONLIVING BIOMASS; DESORPTION	GREEN-ALGA; CHLORELLA-VULGARIS; HEAVY-METALS; C-VULGARIS; ACCUMULATION; TOXICITY; SORPTION; ZINC; BIOSORPTION; BINDING	The freeze-dried (extracted and non-extracted) biomass of 15 microalgal species grown in axenic mass culture and belonging to the Cyanobacteria, Chloro-, Eustigmato-, Phaeo-, Rhodo- and Tribophyceae were investigated for their ability to adsorb cadmium (Cd) ions from aqueous solutions. For comparison, other standard adsorbing materials (activated carbon, silica gel, siliceous earth) were included in the studies. The biomass of 11 microalgae exhibited a higher Cd adsorption than the standard materials. Extraction of the algal biomass increased the Cd adsorption capability of some, but not all microalgae. High Cd adsorption was found in Anabaena lutea, Nodularia harveyana, and Nostoc commune (Cyanobacteria), Chlamydomonas sp. (Chlorophyceae), Bumilleriopsis filiformis (Tribophyceae), and in Ectocarpus siliculosus, Halopteris scoparia and Spermatochnus paradoxus (Phaeophyceae). The specific surface (m(2) cm(-3)) of the various microalgae was determined by means of laser diffractometry. Anabaena inaequalis and A. lutea (Cyanobacteria) and the Phaeophyceae had especially high Cd adsorption per surface unit. Most of the Cd adsorbed to these various materials could be desorbed subsequently with diluted mineral acid (pH 2).		FEHRMANN, C (corresponding author), CHRISTIAN ALBRECHTS UNIV KIEL,INST PHARMAZEUT,PHARMAZEUT BIOL ABT,GUTENBERGSTR 76-78,D-24118 KIEL,GERMANY.						AKSU Z, 1990, ENVIRON TECHNOL, V11, P979, DOI 10.1080/09593339009384950; AKSU Z, 1991, J CHEM TECHNOL BIOT, V52, P109; CAIN JR, 1980, ARCH ENVIRON CON TOX, V9, P9, DOI 10.1007/BF01055495; CANTERFORD GS, 1978, AUST J MAR FRESH RES, V29, P613; CONWAY HL, 1979, J FISH RES BOARD CAN, V36, P579, DOI 10.1139/f79-083; COSSA D, 1976, MAR BIOL, V34, P163, DOI 10.1007/BF00390758; COSTA ACA, 1990, BIOTECHNOL LETT, V12, P941, DOI 10.1007/BF01022595; FISHER NS, 1984, MAR ECOL PROG SER, V18, P201, DOI 10.3354/meps018201; GEISWEID HJ, 1983, Z PFLANZENPHYSIOL, V109, P127, DOI 10.1016/S0044-328X(83)80202-5; HARRIS PO, 1990, ENVIRON SCI TECHNOL, V24, P22; HASSETT JM, 1980, CONTAMINANTS SEDIMEN, V2, P409; JENNETT JC, 1977, TRACE SUBST ENV HLTH, V11, P448; KAYSER H, 1980, HELGOLANDER MEERESUN, V33, P89, DOI 10.1007/BF02414738; KHUMMONGKOL D, 1982, BIOTECHNOL BIOENG, V24, P2643, DOI 10.1002/bit.260241204; KUYUCAK N, 1988, Water Pollution Research Journal of Canada, V23, P424; LES A, 1984, WATER AIR SOIL POLL, V23, P129, DOI 10.1007/BF00206971; MAJIDI V, 1990, ENVIRON SCI TECHNOL, V24, P1309, DOI 10.1021/es00079a002; MYKLESTAD S, 1981, P INT SEAWEED S, V8, P589; MYKLESTAD S, 1969, P INT SEAWEED S, V6, P545; POHL P, 1987, PHYTOCHEMISTRY, V26, P1657, DOI 10.1016/S0031-9422(00)82264-5; POHL P, 1986, PLANTA MED, V52, P416; Pohl P., 1988, ALGAL BIOTECHNOLOGY, P209; SAKAGUCHI T, 1979, EUR J APPL MICROBIOL, V8, P207, DOI 10.1007/BF00506184; SAMI FE, 1983, EGYPT J MICROBIOL, V21, P263; SKOWRONSKI T, 1986, CHEMOSPHERE, V15, P77, DOI 10.1016/0045-6535(86)90581-3; SMITH JE, 1981, INT C, V3, P155; TING YP, 1989, BIOTECHNOL BIOENG, V34, P990, DOI 10.1002/bit.260340713; VOLESKY B, 1987, TRENDS BIOTECHNOL, V5, P96, DOI 10.1016/0167-7799(87)90027-8; VYMAZAL J, 1990, ACTA HYDROCH HYDROB, V18, P657, DOI 10.1002/aheh.19900180605	29	36	40	0	16	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0921-8971			J APPL PHYCOL	J. Appl. Phycol.	DEC	1993	5	6					555	562		10.1007/BF02184634			8	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	Biotechnology & Applied Microbiology; Marine & Freshwater Biology	MN787	WOS:A1993MN78700001					2021-04-07	
J	KATSAROS, CI; MAIER, I; MELKONIAN, M				KATSAROS, CI; MAIER, I; MELKONIAN, M			IMMUNOLOCALIZATION OF CENTRIN IN THE FLAGELLAR APPARATUS OF MALE GAMETES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE) AND OTHER BROWN ALGAL MOTILE CELLS	JOURNAL OF PHYCOLOGY			English	Article						BROWN ALGAL ZOIDS; CENTRIN; CYTOSKELETON; ECTOCARPUS-SILICULOSUS; FLAGELLAR APPARATUS; PHAEOPHYCEAE	BASAL BODY CONNECTOR; GREEN-ALGAE; LAMINARIALES PHAEOPHYCEAE; CHLAMYDOMONAS-REINHARDTII; ABSOLUTE-CONFIGURATION; REPRODUCTIVE STAGES; TRANSITION REGION; DILLW LYNGB; ULTRASTRUCTURE; LOCALIZATION	Centrin or a centrin homologue was localized using immunofluorescence in the flagellar basal body region in zoids of five brown algal species: Ectocarpus siliculosus (Dillw.) Lyngb., Scytosiphon lomentaria (Lyngb.) Link, Laminaria digitata (Huds.) Lamour., Sphacelaria rigidula (Kutz.) Prud'homme van Reine, and Fucus serratus L. The antigen is restricted to short rods extending along the basal body(ies) and toward the nucleus, which always remains firmly linked to the flagellar apparatus in isolated cytoskeletons. To identify these antigenic sites, pre- and postembedding immunogold electron microscopy was applied to male gametes of E. siliculosus. At least three different structures associated with the basal bodies were antigenic: a fibrous structure connecting the proximal end of the posterior basal body to the nucleus (nucleus-basal body connector), a striated band that links the two basal bodies to each other and is located in the angle formed by them, and amorphous material interconnecting the basal bodies in their most proximal regions. In addition, specific labeling occurs along the external surface and within the lumen of both basal bodies and in the flagellar transitional region. The possible function of these centrin-containing structures is discussed.	UNIV COLOGNE,LEHRSTUHL I,INST BOT,D-50931 COLOGNE,GERMANY	KATSAROS, CI (corresponding author), UNIV ATHENS,INST GEN BOT,GR-15784 ATHENS,GREECE.						ANDERSEN RA, 1991, PROTOPLASMA, V164, P143, DOI 10.1007/BF01320820; ANDERSEN RA, 1991, PROTOPLASMA, V164, P1, DOI 10.1007/BF01320809; BAKER JRJ, 1973, PROTOPLASMA, V77, P181, DOI 10.1007/BF01276756; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BOUCK BG, 1969, J CELL BIOL, V40, P446; CHEIGNON M, 1964, CR HEBD ACAD SCI, V258, P676; CLAYTON MN, 1984, J PHYCOL, V20, P276, DOI 10.1111/j.0022-3646.1984.00276.x; CLAYTON MN, 1989, CHROMOPHYTE ALGAE PR, V38, P229; DEMEY J, 1981, CELL BIOL INT REP, V5, P889, DOI 10.1016/0309-1651(81)90204-6; FRENS G, 1973, NATURE-PHYS SCI, V241, P20, DOI 10.1038/physci241020a0; HENRY EC, 1982, J PHYCOL, V18, P570; HENRY EC, 1982, J PHYCOL, V18, P550; HIBBERD DJ, 1979, BIOSYSTEMS, V11, P243, DOI 10.1016/0303-2647(79)90025-X; HOHFELD I, 1988, PROTOPLASMA, V147, P16, DOI 10.1007/BF01403874; JARVIK JW, 1991, J CELL SCI, V99, P731; KATSAROS C, 1991, BOT ACTA, V104, P87, DOI 10.1111/j.1438-8677.1991.tb00201.x; KATSAROS C, 1992, PROTOPLASMA, V169, P75, DOI 10.1007/BF01343372; LACLAIRE JW, 1979, PROTOPLASMA, V101, P247, DOI 10.1007/BF01276967; LACLAIRE JW, 1978, PROTOPLASMA, V97, P93, DOI 10.1007/BF01276686; LECHTRECK KF, 1991, PROTOPLASMA, V164, P38, DOI 10.1007/BF01320813; LOFTHOUSE PF, 1975, PROTOPLASMA, V84, P83, DOI 10.1007/BF02075945; LOISEAUX S, 1973, J PHYCOL, V9, P277; MAIER I, 1990, J EXP BOT, V41, P869, DOI 10.1093/jxb/41.7.869; MAIER I, 1982, PHYCOLOGIA, V21, P1, DOI 10.2216/i0031-8884-21-1-1.1; MANTON I, 1959, J EXP BOT, V10, P448, DOI 10.1093/jxb/10.3.448; MANTON I, 1956, J EXP BOT, V21, P416; MARKEY DR, 1976, PROTOPLASMA, V88, P175, DOI 10.1007/BF01283244; MARKEY DR, 1976, PROTOPLASMA, V88, P147, DOI 10.1007/BF01283243; MARTINDALE VE, 1990, J CELL SCI, V96, P395; MELKONIAN M, 1988, PROTOPLASMA, V144, P56, DOI 10.1007/BF01320280; Melkonian M., 1992, P179; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MOTOMURA T, 1989, Japanese Journal of Phycology, V37, P105; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; OKELLY CJ, 1984, PROTOPLASMA, V123, P18, DOI 10.1007/BF01283178; OKELLY CJ, 1985, PHYCOLOGIA, V24, P263, DOI 10.2216/i0031-8884-24-3-263.1; OKelly CJ, 1989, CHROMOPHYTE ALGAE PR, V38, P255; PAINTRAND M, 1992, J STRUCT BIOL, V108, P107, DOI 10.1016/1047-8477(92)90011-X; PREISIG HR, 1989, CHROMOPHYTE ALGAE PR, V38, P167; Provasoli L., 1968, CULTURES COLLECTIONS, P63; ROTH J, 1982, TECHNIQUES IMMUNOCYT, V1, P107; SALISBURY JL, 1984, J CELL BIOL, V99, P962, DOI 10.1083/jcb.99.3.962; SALISBURY JL, 1989, ALGAE EXPT SYSTEMS, P169; SANDERS MA, 1989, J CELL BIOL, V108, P1751, DOI 10.1083/jcb.108.5.1751; SCHULZE D, 1987, EUR J CELL BIOL, V45, P51; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; TAILLON BE, 1992, J CELL BIOL, V119, P1613, DOI 10.1083/jcb.119.6.1613; VANDENHOEK C, 1968, BLUMEA, V16, P193; VANREINE WFP, 1982, LEIDEN BOTANICAL SER, V6; WRIGHT RL, 1985, J CELL BIOL, V101, P1903, DOI 10.1083/jcb.101.5.1903; WRIGHT RL, 1989, CELL MOTIL CYTOSKEL, V14, P516, DOI 10.1002/cm.970140409	52	26	26	0	2	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	DEC	1993	29	6					787	797		10.1111/j.0022-3646.1993.00787.x			11	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	MR550	WOS:A1993MR55000007					2021-04-07	
J	SCHERER, S; LECHNER, S; BOGER, P				SCHERER, S; LECHNER, S; BOGER, P			PSBD SEQUENCES OF BUMILLERIOPSIS-FILIFORMIS (HETEROKONTOPHYTA, XANTHOPHYCEAE) AND PORPHYRIDIUM-PURPUREUM (RHODOPHYTA, BANGIOPHYCIDAE) - EVIDENCE FOR POLYPHYLETIC ORIGINS OF PLASTIDS	CURRENT GENETICS			English	Article						EVOLUTION; ALGAE; PLASTID; ENDOCYTOBIOSIS; MOLECULAR CLOCK; PSBD	COMPOSITE PHYLOGENETIC ORIGIN; ALGA PYLAIELLA-LITTORALIS; NUCLEOTIDE-SEQUENCE; PHOTOSYSTEM-II; RIBOSOMAL-RNA; CYANIDIUM-CALDARIUM; CHLOROPLAST GENOME; RED ALGA; CHLAMYDOMONAS-REINHARDTII; THYLAKOID MEMBRANE	The nucleotide sequences of the plastidal pshD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A composite DI/D2 protein-similarity network reveals that neither the three red algal sequences nor the two heterokontophyte sequences (Bumilleriopsis, xanthophytes and Ectocarpus, phaeophytes) group together. Therefore, the Heterokontophyta and Rhodophyta may be heterogeneous groups. Instead, it emerges that the DI/D2 proteins of Porphyridium and Bumilleriopsis clearly form a tight cluster. Dl and D2 proteins apparently do not provide a reliable molecular clock. These results fit into hypotheses proposing a polyphyletic origin for complex plastids, even among the supposedly ''natural'' group of heterokontophytes.	UNIV KONSTANZ,LEHRSTUHL PHYSIOL & BIOCHEM PFLANZEN,D-78464 CONSTANCE,GERMANY	SCHERER, S (corresponding author), TECH UNIV MUNICH,INST MIKROBIOL,FML,VOTTINGERSTR 45,D-85354 FREISING,GERMANY.		Scherer, Siegfried/I-1320-2013	Scherer, Siegfried/0000-0002-0394-9890			ALT J, 1984, CURR GENET, V8, P597, DOI 10.1007/BF00395705; ASSALI NE, 1990, PLANT MOL BIOL, V15, P307, DOI 10.1007/BF00036916; ASSALI NE, 1991, PLANT MOL BIOL, V17, P853, DOI 10.1007/BF00037066; BAPLMER JD, 1991, MOL BIOL PLASTIDS, P5; BARBER J, 1987, TRENDS BIOCHEM SCI, V12, P123, DOI 10.1016/0968-0004(87)90058-2; Boger P, 1981, Photosynthesis research, V2, P61, DOI 10.1007/BF00036166; BUKHAROV AA, 1989, NUCLEIC ACIDS RES, V17, P798, DOI 10.1093/nar/17.2.798; CAVALIERSMITH T, 1987, ANN NY ACAD SCI, V503, P55, DOI 10.1111/j.1749-6632.1987.tb40597.x; CAVALIERSMITH T, 1981, BIOSYSTEMS, V14, P461, DOI 10.1016/0303-2647(81)90050-2; CHRISHOLM D, 1988, PLANT MOL BIOL, V10, P293; Dodge JD, 1973, FINE STRUCTURE ALGAL; EFIMOV VA, 1988, NUCLEIC ACIDS RES, V16, P5686, DOI 10.1093/nar/16.12.5686; ERICKSON JM, 1986, EMBO J, V5, P1745, DOI 10.1002/j.1460-2075.1986.tb04422.x; ERICKSON JM, 1984, EMBO J, V3, P2753, DOI 10.1002/j.1460-2075.1984.tb02206.x; FITCH WM, 1967, SCIENCE, V155, P279, DOI 10.1126/science.155.3760.279; GABRIELSON PW, 1985, BIOSYSTEMS, V18, P335, DOI 10.1016/0303-2647(85)90033-4; Garbary DJ, 1990, BIOL RED ALGAE, P477; GIBBS SP, 1981, ANN NY ACAD SCI, V361, P193; GINGRICH JC, 1990, PHOTOSYNTH RES, P137; GOLDEN SS, 1988, GENE, V67, P85, DOI 10.1016/0378-1119(88)90011-X; GRAY MW, 1989, TRENDS GENET, V5, P294, DOI 10.1016/0168-9525(89)90111-X; GRAY MW, 1991, GENETICS PLANTS A, V7, P303; GREEN JC, 1989, CHROMOPHYTE ALGAE PR; HIRATSUKA J, 1989, MOL GEN GENET, V217, P185, DOI 10.1007/BF02464880; HOEK J, 1984, EINFUHRUNG PHYKOLOGI; HOLSCHUH K, 1984, NUCLEIC ACIDS RES, V12, P8819, DOI 10.1093/nar/12.23.8819; HORI H, 1987, MOL BIOL EVOL, V4, P445; JENSEN TE, 1991, ENDOCYT CELL RES, V8, P1; KOCH W, 1953, ARCH MIKROBIOL, V18, P232; KOWALLIK KV, 1989, CHROMOPHYTE ALGAE PR, P101; LIDHOLM J, 1991, MOL GEN GENET, V226, P345, DOI 10.1007/BF00260645; MAERZ M, 1992, CURR GENET, V21, P73, DOI 10.1007/BF00318658; MAID U, 1990, CURR GENET, V17, P255, DOI 10.1007/BF00312617; MAID U, 1991, PLANT MOL BIOL, V16, P537, DOI 10.1007/BF00023420; MAIER UG, 1991, MOL GEN GENET, V230, P155, DOI 10.1007/BF00290663; MARKOWICZ Y, 1991, CURR GENET, V20, P427, DOI 10.1007/BF00317073; MEYEN S. V., 1987, FUNDAMENTALS PALEOBO; OHYAMA K, 1986, NATURE, V322, P572, DOI 10.1038/322572a0; PERASSO R, 1989, NATURE, V339, P142, DOI 10.1038/339142a0; Pueschel C.M., 1990, BIOL RED ALGAE; RASMUSSEN OF, 1984, PLANT MOL BIOL, V3, P191, DOI 10.1007/BF00029654; ROCHAIX JD, 1989, EMBO J, V8, P1013, DOI 10.1002/j.1460-2075.1989.tb03468.x; ROCHAIX JD, 1991, EMBO J, V8, P215; SCHENK HEA, 1992, Z NATURFORSCH C, V47, P387; SCHENK HEA, 1991, ENDOCYT CELL RES, V8, P215; SCHERER S, 1990, EVOL BIOL, V24, P83; SCHERER S, 1991, CURR GENET, V19, P503, DOI 10.1007/BF00312743; SECKBACH J, 1987, ANN NY ACAD SCI, V503, P5424; SHINOZAKI K, 1986, EMBO J, V5, P2043, DOI 10.1002/j.1460-2075.1986.tb04464.x; SITTE P, 1990, Biologische Rundschau, V28, P1; SITTE P, 1992, PROG BOT, V53, P29; SOGIN ML, 1989, SCIENCE, V243, P75, DOI 10.1126/science.2911720; South G. R., 1987, INTRO PHYCOLOGY; TREBST A, 1986, Z NATURFORSCH C, V41, P240; UEDA K, 1987, BRIT PHYCOL J, V22, P61, DOI 10.1080/00071618700650081; VALENTIN K, 1990, MOL GEN GENET, V222, P425, DOI 10.1007/BF00633849; VALENTIN K, 1990, PLANT MOL BIOL, V15, P575, DOI 10.1007/BF00017832; WHATLEY JM, 1981, ANN NY ACAD SCI, V361, P154, DOI 10.1111/j.1749-6632.1981.tb46517.x; WINHAUER T, 1991, CURR GENET, V20, P177, DOI 10.1007/BF00312783; WOLFE KH, 1987, P NATL ACAD SCI USA, V84, P9054, DOI 10.1073/pnas.84.24.9054; ZURAWSKI G, 1982, P NATL ACAD SCI-BIOL, V79, P7699, DOI 10.1073/pnas.79.24.7699	61	3	3	0	2	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0172-8083			CURR GENET	Curr. Genet.	NOV	1993	24	5					437	442		10.1007/BF00351854			6	Genetics & Heredity	Genetics & Heredity	MA658	WOS:A1993MA65800013	8299160				2021-04-07	
J	SCHMID, R; DRING, MJ				SCHMID, R; DRING, MJ			EVIDENCE FOR 2 DIFFERENT BLUE-LIGHT-RECEPTOR SYSTEMS FOR THE FAST RESPONSES OF STIMULATION OF PHOTOSYNTHETIC CAPACITY AND ACIDIFICATION OF THE PLANT-SURFACE IN BROWN-ALGAE	PLANTA			English	Article						ACTION SPECTROSCOPY; BLUE LIGHT (SENSITIVITY); FLAVIN INHIBITOR; PHAEOPHYTA; PHOTOSYNTHESIS; PH SHIFT	SATURATED PHOTOSYNTHESIS; PHAEOPHYTA	Two blue-light responses of Phaeophyta that are expressed within a few seconds of a blue-light stimulus were characterized with respect to their photoreception properties. The first response is the activation of red-light-saturated photosynthesis which can be stimulated to values up to 5 times the rates in red light, depending on the species. The second response is a blue-light-induced acidification measurable at the plant surface. Both responses have similar kinetic characteristics and thus led us initially to hypothesise that they were causally connected in the same transduction mechanism. The two responses have action spectra [measured for Ectocarpus siliculosus (Dillwyn) Lyngb. and Laminaria saccharina (L.) Lamouroux] that are indistinguishable within the relatively large limits of error. However, in all species tested, the threshold sensitivity for blue light of the photosynthetic response is lower than that of the pH-shift by a factor of 2 to 150. Furthermore, stimulation of photosynthesis is sensitive to the flavin inhibitors, KI and phenylacetic acid, but the pH response is not affected by these inhibitors. Thus, the blue-light-induced pH-shift does not cause the stimulation of photosynthesis. In contrast, the different fluence-response relationships of the two responses and particularly the differential effect of the inhibitors are clear evidence for the action of two independent transduction pathways and photoreceptor systems for blue light. At least photoreception for stimulation of photosynthesis involves-a flavin-or and a pterin.		SCHMID, R (corresponding author), QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND.		Dring, Matthew/B-4941-2014	Dring, Matthew/0000-0001-9043-5670			DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; FORSTER RM, 1992, THESIS QUEENS U BELF; GALLAND P, 1985, PHOTOCHEM PHOTOBIOL, V41, P331, DOI 10.1111/j.1751-1097.1985.tb03493.x; GALLAND P, 1988, J PHOTOCH PHOTOBIO B, V1, P277, DOI 10.1016/1011-1344(88)85016-4; GALLAND P, 1988, PHOTOCHEM PHOTOBIOL, V48, P811, DOI 10.1111/j.1751-1097.1988.tb02896.x; GALLAND P, 1987, BLUE LIGHT RESPONSES, P37; Galland Paul, 1991, P65; Goodwin T.W., 1991, P125; HEMMERIC.P, 1967, NATURE, V213, P728, DOI 10.1038/213728a0; HOHL N, 1992, PHOTOCHEM PHOTOBIOL, V55, P239, DOI 10.1111/j.1751-1097.1992.tb04233.x; KARLSSON PE, 1992, PHOTOCHEM PHOTOBIOL, V55, P605, DOI 10.1111/j.1751-1097.1992.tb04283.x; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; SCHMID R, 1993, PLANT PHYSIOL, V101, P907, DOI 10.1104/pp.101.3.907; Senger H., 1986, Photomorphogenesis in plants, P137; SHROPSHIRE W, 1980, BLUE LIGHT SYNDROME, P172; VIERSTRA RD, 1981, PLANT PHYSIOL, V67, P996, DOI 10.1104/pp.67.5.996; WARPEHA KMF, 1992, PHOTOCHEM PHOTOBIOL, V55, P595, DOI 10.1111/j.1751-1097.1992.tb04282.x	18	19	20	0	0	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0032-0935			PLANTA	Planta	SEP	1993	191	4					489	495					7	Plant Sciences	Plant Sciences	LZ767	WOS:A1993LZ76700008					2021-04-07	
J	SANTELICES, B; VARELA, D				SANTELICES, B; VARELA, D			INTRA-CLONAL VARIATION IN THE RED SEAWEED GRACILARIA-CHILENSIS	MARINE BIOLOGY			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; CHONDRUS-CRISPUS RHODOPHYTA; FUNCTIONAL-FORM MODEL; TIKVAHIAE RHODOPHYCEAE; MORPHOLOGICAL MUTANTS; LIFE-HISTORY; ECOTYPIC DIFFERENTIATION; MARINE MACROALGAE; ENDOPHYTIC ALGAE; MIXED PHASES	The phenotypic plasticity often found in seaweed populations has been explained only from the perspective of inter-population or inter-individual differences. However, many seaweeds grow and propagate by fragmentation of genetically identical units, each with the capacity to function on its own. If significant differences in performance exist among these supposedly identical units, such differences should be expressed upon the release and growth of these units. In this study we document two such types of variation in the red seaweed Gracilaria chilensis. Populations of sporelings, each grown under similar culture conditions and derived from carpospores shed by the same cystocarp exhibit significant differences in growth. In this species, each cystocarp develops from a simple gametic fusion, and cystocarp fusions occur too infrequently to account for the growth differences observed among recruits. In adult thalli, branches (ramets) derived from the same thallus (genet) and grown under similar conditions exhibit significant variation in growth rates and morphology. These findings have several implications. They suggest that carpospore production is not only an example of zygote amplification but that it also could increase variability among mitotically replicated units. Intra-clonal variability followed by fragmentation and re-attachment may increase intra-population variation which, in species of Gracilaria, is often larger than inter-population variation. In addition, the existence of intra-clonal variability suggests that strain selection in commercially important species may require a more continuous screening of high-quality strains because of frequent genotypic or phenotypic changes in the various cultivars.		SANTELICES, B (corresponding author), CATHOLIC UNIV CHILE, FAC CIENCIAS BIOL, DEPT ECOL, SANTIAGO, CHILE.		Varela, Daniel/D-7908-2013	Varela, Daniel/0000-0003-4603-4970			BOLTON JJ, 1983, PHYCOLOGIA, V22, P133, DOI 10.2216/i0031-8884-22-2-133.1; BONGA JM, 1985, TISSUE CULTURE FORES; Buss L.W., 1985, P467; CARROLL MA, 1991, J PHYCOL S, V21, P13; COLLANTES GE, 1990, ARCH BIOL MED EXP, V23, P131; Cook RE, 1985, POPULATION BIOL EVOL, P259; CORREA JA, 1988, J PHYCOL, V24, P528, DOI 10.1111/j.1529-8817.1988.tb04258.x; CORREA JA, 1991, J PHYCOL, V27, P448, DOI 10.1111/j.0022-3646.1991.00448.x; DURAKO MJ, 1980, MAR BIOL, V59, P151, DOI 10.1007/BF00396862; ESPINOZA J, 1983, MAR BIOL, V74, P213, DOI 10.1007/BF00413924; FREDERICQ S, 1989, J PHYCOL, V25, P213, DOI 10.1111/j.1529-8817.1989.tb00116.x; GERARD VA, 1988, MAR BIOL, V97, P25, DOI 10.1007/BF00391242; GUIRY MD, 1978, MODERN APPROACHES TA, V10, P111; HANISAK MD, 1984, HYDROBIOLOGIA, V116, P295; HANISAK MD, 1990, HYDROBIOLOGIA, V204, P73, DOI 10.1007/BF00040217; HANISAK MD, 1988, MAR BIOL, V99, P157, DOI 10.1007/BF00391977; Harper J.L., 1985, P1; HARPER JL, 1986, PHILOS T ROY SOC B, V313, P3, DOI 10.1098/rstb.1986.0021; Hawkes M. W., 1990, BIOL RED ALGAE, P455; HODGSON LM, 1984, J PHYCOL, V20, P444, DOI 10.1111/j.0022-3646.1984.00444.x; HOMMERSAND MH, 1988, PHYCOLOGIA, V27, P254, DOI 10.2216/i0031-8884-27-2-254.1; Jackson JBC, 1985, POPULATION BIOL EVOL, pix; LIGNELL A, 1989, BOT MAR, V32, P219, DOI 10.1515/botm.1989.32.3.219; LUNING K, 1980, SHORE ENV, V2, P915; McLachlan J., 1973, HDB PHYCOLOGICAL MET, P25; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; PATWARY MU, 1983, AQUACULTURE, V33, P207, DOI 10.1016/0044-8486(83)90401-5; PATWARY MU, 1982, CAN J BOT, V60, P2556, DOI 10.1139/b82-310; PATWARY MU, 1983, CAN J BOT, V61, P1654, DOI 10.1139/b83-177; PATWARY MU, 1983, BOT MAR, V26, P295, DOI 10.1515/botm.1983.26.6.295; PECKOL P, 1985, BOT MAR, V28, P319, DOI 10.1515/botm.1985.28.8.319; PRIETO I, 1991, REV CHIL HIST NAT, V64, P343; RICE EL, 1985, MAR BIOL, V88, P207, DOI 10.1007/BF00397168; RUSSELL G, 1986, OCEANOGR MAR BIOL, V24, P309; SANTELICES B, 1989, AQUACULTURE, V78, P95, DOI 10.1016/0044-8486(89)90026-4; SEARLES RB, 1980, AM NAT, V115, P113, DOI 10.1086/283548; SHEATH RG, 1984, PHYCOLOGIA, V23, P383, DOI 10.2216/i0031-8884-23-3-383.1; SIEGEL S, 1988, NONPARAMETRIC STATIS; Silander J. A. J, 1985, POPULATION BIOL EVOL, P107; Snedecor G.W., 1967, STATISTICAL METHODS; VANDERMEER JP, 1987, HYDROBIOLOGIA, V151, P49, DOI 10.1007/BF00046104; VANDERMEER JP, 1988, J PHYCOL, V24, P198; VANDERMEER JP, 1977, CAN J BOT, V55, P2810, DOI 10.1139/b77-319; WATKINSON AR, 1986, PHILOS T R SOC B, V313, P31, DOI 10.1098/rstb.1986.0024	47	38	38	0	13	SPRINGER HEIDELBERG	HEIDELBERG	TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY	0025-3162	1432-1793		MAR BIOL	Mar. Biol.	AUG	1993	116	4					543	552		10.1007/BF00355472			10	Marine & Freshwater Biology	Marine & Freshwater Biology	LR573	WOS:A1993LR57300003					2021-04-07	
J	MULLER, DG; FRENZER, K				MULLER, DG; FRENZER, K			VIRUS-INFECTIONS IN 3 MARINE BROWN-ALGAE - FELDMANNIA-IRREGULARIS, F-SIMPLEX, AND ECTOCARPUS-SILICULOSUS	HYDROBIOLOGIA			English	Article; Proceedings Paper	14TH INTERNATIONAL SEAWEED SYMP	AUG 16-21, 1992	BREST, FRANCE	INT SEAWEED ASSOC, MARINALG INT, CHINA SEEWEED IND ASSOC, COPENHAGEN PECTIN, GRIDSTED PROD, MERCK & CO, KELCO DIV, MAROKAGAR, PROTAN BIOPOLYM, QUEST BIOCON, SANOFI BIO IND		VIRUS INFECTION; MARINE; PHAEOPHYCEAE; FELDMANNIA; ECTOCARPUS	PHAEOPHYCEAE	Culture studies with healthy and virus-infected isolates of Ectocarpus siliculosus, Feldmannia simplex and F. irregularis gave the following results: Virus particles are produced in deformed reproductive organs (sporangia or gametangia) of the hosts and are released into the surrounding seawater. Their infective potential is lost after several days of storage under laboratory conditions. New infections occur when gametes or spores of the host get in contact with virus particles. The virus genome enters all cells of the developing new plant via mitosis. Virus expression is variable, and in many cases the viability of the host is not impaired. Infected host plants may be partly fertile and pass the infection to their daughter plants. Meiosis of the host can eliminate the virus genome and generate healthy progeny. The genome of the Ectocarpus virus consists of dsDNA. Meiotic segregation patterns suggest an intimate association between virus genome and host chromosomes. An extra-generic host range has been demonstrated for the Ectocarpus virus. Field observations suggest that virus infections in ectocarpalean algae occur on all coasts of the world, and many or all Ectocarpus and Feldmannia populations are subject to contact with virus genomes.		MULLER, DG (corresponding author), UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY.						L?ning K., 1990, SEAWEEDS THEIR ENV B; LANKA S, 1993, IN PRESS VIROLOGY; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; REANNEY DC, 1974, INT REV CYTOL, V37, P21, DOI 10.1016/S0074-7696(08)61356-X; Starr R.C., 1987, Journal of Phycology, V23, P1; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991	10	8	8	0	4	KLUWER ACADEMIC PUBL	DORDRECHT	SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS	0018-8158			HYDROBIOLOGIA	Hydrobiologia	JUN 18	1993	261						37	44					8	Marine & Freshwater Biology	Marine & Freshwater Biology	LR168	WOS:A1993LR16800005					2021-04-07	
J	SCHMID, CE				SCHMID, CE			CELL-CELL-RECOGNITION DURING FERTILIZATION IN ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	HYDROBIOLOGIA			English	Article						CELL-CELL-RECOGNITION; FERTILIZATION; ECTOCARPUS; LECTIN; COMMUNICATION; SPECIES-SPECIFICITY; WGA	BROWN-ALGAE	Two cellular communication systems are involved in sexual recognition of complementary gametes of Ectocarpus siliculosus. The first is mediated via the pheromone ectocarpene and is not species-specific. A second system has been identified which is based on the interaction of carbohydrate and carbohydrate receptor. Cell fusion is inhibited selectively by wheat germ agglutinin (WGA) applied to female gametes or with the complementary N-acetylglucosamine (GlcNAc) applied to male gametes. Both, WGA and GlcNAc showed gametic specificity in their effects. Fertilization was not affected by the lectins GNA, LCA and SBA. The maximum fertilization success was 50.2% and followed Michaelis-Menten-kinetics (I50 = 6 minutes).		SCHMID, CE (corresponding author), UNIV CONSTANCE, FAK BIOL, W-7750 CONSTANCE, GERMANY.						BOLWELL GP, 1977, NATURE, V268, P626, DOI 10.1038/268626a0; BOLWELL GP, 1979, J CELL SCI, V36, P19; CATT JW, 1983, EXP CELL RES, V147, P127, DOI 10.1016/0014-4827(83)90277-X; DESTOMBE C, 1990, PHYCOLOGIA, V29, P316, DOI 10.2216/i0031-8884-29-3-316.1; GELLER A, 1981, J EXP BIOL, V92, P53; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; MULLER DG, 1985, Z NATURFORSCH C, V40, P457; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; PIERCE M, 1983, J BIOL CHEM, V258, P3576; SCHMID CE, 1992, IN PRESS PLANT PHYSL, V30; STACHE B, 1990, NATO ASI SERIES G, V22, P173; STARR RC, 1987, J PHYCOL S, V23, P35; TRIMMER JS, 1985, CELL, V40, P697, DOI 10.1016/0092-8674(85)90218-1; WASSARMAN PM, 1989, CIBA F SYMP, V145, P135	19	21	21	0	3	SPRINGER	DORDRECHT	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS	0018-8158	1573-5117		HYDROBIOLOGIA	Hydrobiologia	JUN 18	1993	261						437	443					7	Marine & Freshwater Biology	Marine & Freshwater Biology	LR168	WOS:A1993LR16800057					2021-04-07	
J	STRATMANN, K; BOLAND, W; MULLER, DG				STRATMANN, K; BOLAND, W; MULLER, DG			BIOSYNTHESIS OF PHEROMONES IN FEMALE GAMETES OF MARINE BROWN-ALGAE (PHAEOPHYCEAE)	TETRAHEDRON			English	Article							CUTLERIA-MULTIFIDA; ECTOCARPUS-SILICULOSUS; GIFFORDIA-MITCHELLAE; SENECIO-ISATIDEUS; HYDROCARBONS; MODEL	Female gametes of the brown algae Ectocarpus siliculosus and Sphacelaria rigidula as well as thalli of Giffordia mitchellae metabolise externally added [H-2(n)]icosanoic acids into the hydrocarbon pheromones ectocarpene (1), dictyotene (2) and finavarrene (5). The series of the C11H16 hydrocarbons originates from all-cis-5,8,11,-14,17-icosapentaenoic acid (7); the C11H18 compound dictyotene (2) is produced from all-cis-5,8,11,14-icosate-traenoic acid (8) (arachidonic acid). The key step in the biosynthesis of giffordene (6) is a thermally allowed [1,7]-hydrogen shift of an 1,3Z,5Z,8Z-undecatetraene (21) intermediate derived from 7.	UNIV KARLSRUHE,INST ORGAN CHEM,RICHARD WILLSTATTER ALLEE 2,W-7500 KARLSRUHE,GERMANY; UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY			Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			ABRAHAM WD, 1991, J AM CHEM SOC, V113, P2313, DOI 10.1021/ja00006a066; BOHLMANN F, 1961, CHEM BER-RECL, V94, P948, DOI 10.1002/cber.19610940411; BOHLMANN F, 1979, PHYTOCHEMISTRY, V18, P9; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLAND W, 1985, EUR J BIOCHEM, V147, P83, DOI 10.1111/j.1432-1033.1985.tb08722.x; BOLAND W, 1987, EXPERIENTIA, V43, P466, DOI 10.1007/BF01940458; BOLAND W, 1987, HELV CHIM ACTA, V70, P1025, DOI 10.1002/hlca.19870700415; Boland W, 1984, ANAL VOLATILES, P371; BOLAND W, 1987, BIOL UNSERER ZEIT, V17, P176; BOLAND W, UNPUB; GROB K, 1976, J CHROMATOGR, V117, P285, DOI 10.1016/0021-9673(76)80005-2; JAENICKE L, 1974, J AM CHEM SOC, V96, P3324, DOI 10.1021/ja00817a056; KEITEL J, 1990, HELV CHIM ACTA, V73, P2101, DOI 10.1002/hlca.19900730806; KLENK E, 1963, H-S Z PHYSIOL CHEM, V334, P44, DOI 10.1515/bchm2.1963.334.1.44; KRAUSE A, 1990, THESIS U KARLSRUHE; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MOORE RE, 1977, ACCOUNTS CHEM RES, V10, P40, DOI 10.1021/ar50110a002; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; MULLER DG, 1985, Z NATURFORSCH C, V40, P457; NEUMANN C, 1990, EUR J BIOCHEM, V191, P453, DOI 10.1111/j.1432-1033.1990.tb19143.x; SCHMID CE, 1991, BIOL CHEM HOPPESEYLE, V372, P450; STRATMANN K, 1992, ANGEW CHEM INT EDIT, V31, P1246, DOI 10.1002/anie.199212461; STRATMANN K, 1992, THESIS U KALRSRUHE; WOOLARD FX, 1975, J CHEM SOC CHEM COMM, P486, DOI 10.1039/c39750000486; WURZENBERGER M, 1984, BIOCHIM BIOPHYS ACTA, V795, P163, DOI 10.1016/0005-2760(84)90117-6; YAMADA K, 1986, TETRAHEDRON, V42, P3775, DOI 10.1016/S0040-4020(01)87531-1; YUST G, 1986, J ORG CHEM, V51, P4799	27	34	35	0	9	PERGAMON-ELSEVIER SCIENCE LTD	OXFORD	THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB	0040-4020			TETRAHEDRON	Tetrahedron	APR 30	1993	49	18					3755	3766		10.1016/S0040-4020(01)90228-5			12	Chemistry, Organic	Chemistry	LB174	WOS:A1993LB17400004					2021-04-07	
J	LANKA, STJ; KLEIN, M; RAMSPERGER, U; MULLER, DG; KNIPPERS, R				LANKA, STJ; KLEIN, M; RAMSPERGER, U; MULLER, DG; KNIPPERS, R			GENOME STRUCTURE OF A VIRUS INFECTING THE MARINE BROWN ALGA ECTOCARPUS-SILICULOSUS	VIROLOGY			English	Article							PULSED-FIELD ELECTROPHORESIS; POLYACRYLAMIDE GELS; GREEN-ALGA; DNA; PHAEOPHYCEAE; MOLECULES; PROTEINS		UNIV CONSTANCE,DIV BIOL,W-7750 CONSTANCE,GERMANY							DUBOCHET J, 1982, ADV OPTICAL ELECTRON, V8, P107; FLATAU E, 1984, MOL CELL BIOL, V4, P2098, DOI 10.1128/MCB.4.10.2098; GERSHONI JM, 1985, ANAL BIOCHEM, V146, P59, DOI 10.1016/0003-2697(85)90395-1; HASCHEME.RH, 1970, ADV ENZYMOL REL S BI, V33, P71; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; LEVENE SD, 1987, P NATL ACAD SCI USA, V84, P4054, DOI 10.1073/pnas.84.12.4054; Louie D, 1989, Appl Theor Electrophor, V1, P169; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; Plattner H, 1987, ELEKTRONENMIKROSKOPI; ROHOZINSKI J, 1989, VIROLOGY, V168, P363, DOI 10.1016/0042-6822(89)90277-8; Sambrook J, 1989, MOL CLONING LABORATO; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; Starr R.C., 1987, Journal of Phycology, V23, P1; Symonds N, 1987, PHAGE MU; TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P4350, DOI 10.1073/pnas.76.9.4350; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VOLLENWEIDER HJ, 1975, P NATL ACAD SCI USA, V72, P83, DOI 10.1073/pnas.72.1.83; WATERBURY PG, 1987, NUCLEIC ACIDS RES, V15, P3930, DOI 10.1093/nar/15.9.3930; WESSEL R, 1990, EUR J BIOCHEM, V189, P277, DOI 10.1111/j.1432-1033.1990.tb15487.x; WRAY W, 1981, ANAL BIOCHEM, V118, P197, DOI 10.1016/0003-2697(81)90179-2; XIA YN, 1987, NUCLEIC ACIDS RES, V15, P6075, DOI 10.1093/nar/15.15.6075; XIA YN, 1986, MOL CELL BIOL, V6, P1440, DOI 10.1128/MCB.6.5.1440; XIA YN, 1988, NUCLEIC ACIDS RES, V16, P9477, DOI 10.1093/nar/16.20.9477; ZIEGLER A, 1987, J CLIN CHEM CLIN BIO, V25, P578	29	45	46	0	9	ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495	0042-6822			VIROLOGY	Virology	APR	1993	193	2					802	811		10.1006/viro.1993.1189			10	Virology	Virology	KT924	WOS:A1993KT92400026	8460486				2021-04-07	
J	SCHMID, R; DRING, MJ				SCHMID, R; DRING, MJ			RAPID, BLUE-LIGHT-INDUCED ACIDIFICATIONS AT THE SURFACE OF ECTOCARPUS AND OTHER MARINE MACROALGAE	PLANT PHYSIOLOGY			English	Article							GUARD-CELL PROTOPLASTS; PEA EPICOTYL; VICIA-FABA; ASSIMILATION; GROWTH	In most brown algae, photosynthesis saturated with red light can be stimulated by continuous blue light. Pulses of blue light lead to transient increases in photosynthetic rate. When a CO2-sensitive electrode was used, occasionally blue light was observed to cause an apparent increase of CO2 instead of the expected decrease. This was changed by buffering the seawater medium and, under these conditions, blue light caused stimulation of CO2 consumption. These results led to investigations of blue-light-dependent pH changes at the outer surface of the plants. Shifts of the pH were recorded in the presence of the photosynthetic inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. In all brown algae tested and in the green algae Ulva and Enteromorpha, blue-light pulses caused transient acidification of 0.03 to 0.18 pH units, depending on the species. The kinetics showed lag phases of a few seconds and the minimum was reached after 5 to 9 min. Fluence response relationships indicated that the sensitivity (threshold) to blue light was very similar in all species. The responses in Ectocarpus changed with time, and about 5 h after the beginning of red light or darkness, a second component became evident, which peaked 20 min after the blue-light pulse. The refractory period of the whole system was about 3 h in Ectocarpus. The blue-light-dependent pH changes show striking similarities to those of higher plant guard cells, and it is possible that similar responses may occur in other tissues of higher plants. In red algae, however, no blue-light-dependent acidifications could be detected The possible role of the observed pH shifts in a mechanism of CO2 acquisition is discussed.		SCHMID, R (corresponding author), QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND.		Dring, Matthew/B-4941-2014	Dring, Matthew/0000-0001-9043-5670			ASSMANN SM, 1985, NATURE, V318, P285, DOI 10.1038/318285a0; BASKIN TI, 1986, PLANTA, V169, P406, DOI 10.1007/BF00392138; DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; ELZENGA JTM, 1989, PLANT PHYSIOL, V91, P62, DOI 10.1104/pp.91.1.62; FISAHN J, 1990, J MEMBRANE BIOL, V113, P23, DOI 10.1007/BF01869602; FORSTER R M, 1990, British Phycological Journal, V25, P87; FORSTER RM, 1992, PLANT CELL ENVIRON, V15, P241, DOI 10.1111/j.1365-3040.1992.tb01478.x; FORSTER RM, 1992, THESIS QUEENS U BELF; GAUTIER H, 1992, PLANT PHYSIOL, V98, P34, DOI 10.1104/pp.98.1.34; JOHNSTON AM, 1990, CAN J BOT, V69, P1123; LASKOWSKI MJ, 1989, PLANT PHYSIOL, V89, P293, DOI 10.1104/pp.89.1.293; LUCAS WJ, 1983, ANNU REV PLANT PHYS, V34, P71, DOI 10.1146/annurev.pp.34.060183.000443; MADSEN TV, 1991, AQUAT BOT, V41, P5, DOI 10.1016/0304-3770(91)90037-6; Provasoli L., 1968, CULTURES COLLECTIONS, P63; RAGHAVENDRA AS, 1990, PLANT CELL ENVIRON, V13, P105, DOI 10.1111/j.1365-3040.1990.tb01282.x; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1992, PLANTA, V187, P53, DOI 10.1007/BF00201623; Senger H., 1986, Photomorphogenesis in plants, P137; SENGER H, 1987, BLUE LIGHT RESPONSES, V2; Senger H., 1987, BLUE LIGHT RESPONSES, V1; SHIMAZAKI K, 1986, NATURE, V319, P324, DOI 10.1038/319324a0; Skirrow G., 1975, CHEM OCEANOGR, V2, P1; TAKESHIGE K, 1992, PLANTA, V186, P466, DOI 10.1007/BF00195329; ZIEGER E, 1990, PLANT CELL ENVIRON, V13, P739	24	21	21	0	5	AMER SOC PLANT PHYSIOLOGISTS	ROCKVILLE	15501 MONONA DRIVE, ROCKVILLE, MD 20855	0032-0889			PLANT PHYSIOL	Plant Physiol.	MAR	1993	101	3					907	913		10.1104/pp.101.3.907			7	Plant Sciences	Plant Sciences	KR450	WOS:A1993KR45000024	12231742	Bronze, Green Published			2021-04-07	
J	FLAVIER, AB; ZINGMARK, RG				FLAVIER, AB; ZINGMARK, RG			MACROALGAL RECRUITMENT IN A HIGH MARSH CREEK OF NORTH INLET ESTUARY, SOUTH-CAROLINA	JOURNAL OF PHYCOLOGY			English	Article						BENTHIC; ESTUARY; MACROALGAL RECRUITMENT; MARINE ECOLOGY; POSTSETTLEMENT SURVIVAL; PROPAGULE SETTLEMENT; SEASONALITY; SPATIAL AND TEMPORAL DISTRIBUTION	INTER-TIDAL COMMUNITY; RED ALGAE; DISPERSAL; SURVIVAL; SPORES; SHORE; FIELD	The temporal and spatial variability in recruitment patterns of macroalgae were studied by evaluating the appearance of propagules on marble tiles set over a depth gradient in a high marsh tidal creek in North Inlet Estuary, South Carolina. Ulvoids and Porphyra occurred over a wide range of depths in the intertidal zone unlike Ectocarpus and members of the Florideophyceae, which grew over a more restricted zone. Ulvoid propagules were the most abundant, attaining maximum densities at 0 and -15 cm mean low water (MLW) at all seasons except winter. In summer, coincident with high turbidity, ulvoid densities decreased at -15 cm MLW but not in other seasons. Ulvoid densities at 0 MLW were depressed in january, February, and July 1990. The decrease in densities in january and February was preceded with the lowest recorded temperatures and salinities during the study period, while the decrease in July was preceded by the highest recorded temperature. The number of taxa was highest in April and September, representing winter-spring and summer-fall transition periods, respectively. The biomass of adult forms was higher on the tiles than on naturally occurring substrata, perhaps due to lack of stable suitable substrata in the field. Inhibition of subsequent recruitment by initial recruitment was evident only on tiles submerged in October. The initial recruitment pattern of Porphyra rosengurtii Coll et Cox and Ectocarpus siliculosus (Dillwyn) Lyngbye seem to determine the biomass distribution of adult forms, while that of ulvoids may be altered later by other factors. The considerable decrease in the number of adult forms compared to the initial densities of propagules indicates high juvenile mortalities.	UNIV S CAROLINA, DEPT BIOL SCI, COLUMBIA, SC 29208 USA; UNIV S CAROLINA, BELLE W BARUCH INST MARINE BIOL & COASTAL RES, COLUMBIA, SC 29208 USA							AMSLER CD, 1980, J PHYCOL, V16, P617, DOI 10.1111/j.1529-8817.1980.tb03080.x; BAKER SM, 1910, NEW PHYTOL, V9, P55; BRAWLEY SH, 1991, J PHYCOL, V27, P179, DOI 10.1111/j.0022-3646.1991.00179.x; CHRISTIE AO, 1962, NATURE, V193, P193, DOI 10.1038/193193a0; COUTINHO R, 1987, THESIS U S CAROLINA; DAYTON PK, 1973, ECOLOGY, V54, P433, DOI 10.2307/1934353; EBELING DE, 1982, THESIS U S CAROLINA; FRECH WL, 1977, THESIS U S CAROLINA; HARGER BWW, 1981, P INT SEAWEED S, V8, P342; HOFFMANN AJ, 1987, BOT MAR, V30, P151, DOI 10.1515/botm.1987.30.2.151; HRUBY T, 1979, J ECOL, V67, P65, DOI 10.2307/2259337; KAPRAUN DF, 1980, BOT MAR, V23, P449; KENELLY SJ, 1983, AQUAT BOT, V17, P275; KJERFVE B, 1978, LIMNOL OCEANOGR, V23, P816, DOI 10.4319/lo.1978.23.4.0816; Knox G. A., 1986, ESTUARINE ECOSYSTEMS, VI; LUBCHENCO J, 1978, AM NAT, V112, P23, DOI 10.1086/283250; NEUSHUL M, 1976, J PHYCOL, V12, P397, DOI 10.1111/j.0022-3646.1976.00397.x; NORTON TA, 1981, BOTANICAL MONOGRAPHS, V17, P421; PREGNALL AM, 1985, MAR ECOL PROG SER, V24, P167, DOI 10.3354/meps024167; RAO MU, 1983, J EXP MAR BIOL ECOL, V70, P45, DOI 10.1016/0022-0981(83)90147-8; REED DC, 1988, ECOL MONOGR, V58, P321, DOI 10.2307/1942543; RICHARDSON JP, 1987, B MAR SCI, V40, P210; ROBLES CD, 1981, ECOLOGY, V62, P1536, DOI 10.2307/1941510; SANTELICES B, 1990, OCEANOGR MAR BIOL, V28, P177; SCHONBECK M, 1978, J EXP MAR BIOL ECOL, V31, P303, DOI 10.1016/0022-0981(78)90065-5; WOODHEAD P, 1975, British Phycological Journal, V10, P269; YARISH C, 1982, PHYCOLOGIA, V21, P112, DOI 10.2216/i0031-8884-21-2-112.1; ZINGMARK RG, 1977, J PHYCOL, V13, P77	28	6	6	0	6	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	FEB	1993	29	1					2	8		10.1111/j.1529-8817.1993.tb00273.x			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	KP046	WOS:A1993KP04600001					2021-04-07	
J	BRZEZINSKI, MA; REED, DC; AMSLER, CD				BRZEZINSKI, MA; REED, DC; AMSLER, CD			NEUTRAL LIPIDS AS MAJOR STORAGE PRODUCTS IN ZOOSPORES OF THE GIANT-KELP MACROCYSTIS-PYRIFERA (PHAEOPHYCEAE)	JOURNAL OF PHYCOLOGY			English	Article						CARBON RESERVE; GAMETE; GERMINATION; KELP; MACROCYSTIS-PYRIFERA; NEUTRAL LIPID; PHAEOPHYCEAE; TRIACYLGLYCEROLS; ZOOSPORE	FOULING ALGA ECTOCARPUS; PTERYGOPHORA-CALIFORNICA; COMMUNITY STRUCTURE; REPRODUCTIVE STAGES; SEA-URCHIN; NILE RED; ULTRASTRUCTURE; RECRUITMENT; SETTLEMENT; DISPERSAL	Neutral lipids, consisting primarily of triacylglycerols, were found to be a major form of carbon reserve in zoospores of the giant kelp Macrocystis pyrifera (L.) C. Ag. The fluorescent stain Nile Red revealed large lipid droplets in the posterior end of the cell, which comprised 20-41% of cellular carbon in newly released spores. Flow cytometric analyses of newly released spores stained with Nile Red revealed considerable variation in the neutral lipid content among spores that was independent of spore size. Lipid droplets were consumed during germination in spores maintained either under constant light or in continual darkness. The availability of light appeared to delay, but did not preclude, lipid use. The rate of lipid use during germination varied considerably among germlings with some cells consuming all of their lipid reserves within 5 h after release. In addition to zoospores, lipid droplets were observed in both male and female gametes. Numerous droplets were observed in eggs, while single lipid droplets were observed in sperm. Neutral lipid droplets were not observed in gametophytes or sporophytes except in developing gametes and spores. Large lipid reserves thus seem to be confined to the microscopic life history stages that presumably have relatively high energy demands. By serving as a supplemental fuel reserve, neutral lipids may be important in extending the effective range of zoospore dispersal.	UNIV CALIF SANTA BARBARA, INST MARINE SCI, SANTA BARBARA, CA 93106 USA; UNIV ILLINOIS, DEPT MICROBIOL & IMMUNOL MC 790, CHICAGO, IL 60680 USA	BRZEZINSKI, MA (corresponding author), UNIV CALIF SANTA BARBARA, DEPT BIOL SCI, SANTA BARBARA, CA 93106 USA.						AMENTA JS, 1964, J LIPID RES, V5, P270; AMSLER CD, 1990, MAR BIOL, V107, P297, DOI 10.1007/BF01319829; AMSLER CD, 1991, J PHYCOL, V27, P26, DOI 10.1111/j.0022-3646.1991.00026.x; AMSLER CD, 1992, BRIT PHYCOL J, V27, P253, DOI 10.1080/00071619200650251; AMSLER CD, 1989, MAR BIOL, V102, P557, DOI 10.1007/BF00438358; BAKER JRJ, 1973, PROTOPLASMA, V77, P181, DOI 10.1007/BF01276756; BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BARRALES HL, 1975, J ECOL, V63, P657, DOI 10.2307/2258743; BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911; CHEN CP, 1991, MAR BIOL, V109, P453, DOI 10.1007/BF01313510; CHI EY, 1972, P INT SEAWEED S, V7, P181; CLAYTON MN, 1992, BRIT PHYCOL J, V27, P219, DOI 10.1080/00071619200650231; CLAYTON MN, 1991, BRIT PHYCOL J, V26, P279, DOI 10.1080/00071619100650241; COOKSEY KE, 1987, J MICROBIOL METH, V6, P333, DOI 10.1016/0167-7012(87)90019-4; COWEN RK, 1982, J EXP MAR BIOL ECOL, V64, P189, DOI 10.1016/0022-0981(82)90152-6; DAYTON PK, 1985, ECOL MONOGR, V55, P447, DOI 10.2307/2937131; DAYTON PK, 1984, SCIENCE, V224, P283, DOI 10.1126/science.224.4646.283; DEAN TA, 1984, MAR BIOL, V78, P301, DOI 10.1007/BF00393016; EBELING AW, 1985, MAR BIOL, V84, P287, DOI 10.1007/BF00392498; Foster M. S., 1982, SYNTHETIC DEGRADATIV, P185; GHERARDINI GL, 1972, P INT SEAWEED S, V7, P172; GREENSPAN P, 1985, J CELL BIOL, V100, P965, DOI 10.1083/jcb.100.3.965; HARMS J, 1991, J EXP MAR BIOL ECOL, V145, P233, DOI 10.1016/0022-0981(91)90178-Y; HARROLD C, 1985, ECOLOGY, V66, P1160, DOI 10.2307/1939168; HENRY EC, 1982, J PHYCOL, V18, P570; HENRY EC, 1982, J PHYCOL, V18, P550; HOFFMANN AJ, 1987, BOT MAR, V30, P151, DOI 10.1515/botm.1987.30.2.151; HOLLAND DL, 1973, J MAR BIOL ASSOC UK, V53, P833, DOI 10.1017/S0025315400022505; HUANG AHC, 1992, ANNU REV PLANT PHYS, V43, P177, DOI 10.1146/annurev.pp.43.060192.001141; HUANG LS, 1990, PHYTOCHEMISTRY, V29, P1441, DOI 10.1016/0031-9422(90)80097-Z; JOHNSON CR, 1988, ECOL MONOGR, V58, P129, DOI 10.2307/1942464; Kates M, 1986, TECHNIQUES LIPIDOLOG; Kessler J.O., 1986, Progress phycol. Res., V4, P257; KESSLER JO, 1985, NATURE, V313, P218, DOI 10.1038/313218a0; LEIGHTON DL, 1966, P INT SEAWEED S, V5, P141; MILLER RJ, 1985, MAR BIOL, V84, P275, DOI 10.1007/BF00392497; MILLS GL, 1974, PHYTOCHEMISTRY, V13, P2653, DOI 10.1016/0031-9422(74)80219-0; NORTON TA, 1992, BRIT PHYCOL J, V27, P293, DOI 10.1080/00071619200650271; NOZZOLILLO C, 1986, AM J BOT, V73, P96, DOI 10.2307/2444282; OLIVEIRA L, 1980, PROTOPLASMA, V104, P1, DOI 10.1007/BF01279364; OUELLET P, 1992, CAN J FISH AQUAT SCI, V49, P368, DOI 10.1139/f92-042; Provasoli L., 1968, CULTURES COLLECTIONS, P63; REED DC, 1991, J PHYCOL, V27, P361, DOI 10.1111/j.0022-3646.1991.00361.x; REED DC, 1992, ECOLOGY, V73, P1577, DOI 10.2307/1940011; REED DC, 1988, ECOL MONOGR, V58, P321, DOI 10.2307/1942543; Reisener H.J., 1976, FUNGAL SPORE FORM FU, P165; RICHMOND RH, 1987, MAR BIOL, V93, P527, DOI 10.1007/BF00392790; SANTELICES B, 1990, OCEANOGR MAR BIOL, V28, P177; SUBERKROPP KF, 1973, ARCH MIKROBIOL, V89, P205, DOI 10.1007/BF00422201; TOTH R, 1976, J PHYCOL, V12, P222; VERESHCHAGIN AG, 1991, PLANT PHYSIOL BIOCH, V29, P385; WEBER DJ, 1980, LIPID BIOCH FUNGI OT, P300; Weete J., 1980, LIPID BIOCH FUNGI OT	53	31	33	0	7	WILEY-BLACKWELL	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0022-3646	1529-8817		J PHYCOL	J. Phycol.	FEB	1993	29	1					16	23		10.1111/j.1529-8817.1993.tb00275.x			8	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	KP046	WOS:A1993KP04600003					2021-04-07	
J	MULLER, DG; PARODI, E				MULLER, DG; PARODI, E			TRANSFER OF A MARINE DNA VIRUS FROM ECTOCARPUS TO FELDMANNIA (ECTOCARPALES, PHAEOPHYCEAE) - ABERRANT SYMPTOMS AND RESTITUTION OF THE HOST	PROTOPLASMA			English	Article						ECTOCARPUS; FELDMANNIA; PHAEOPHYCEAE; DNA-VIRUS, MARINE; INTERGENERIC GENE TRANSFER	SILICULOSUS PHAEOPHYCEAE; INFECTION	The marine brown alga Ectocarpus siliculosus is invaded by a polyhedric virus, whose genome consists of circular, double-stranded DNA. In laboratory experiments this virus can infect a different host species, Feldnannia simplex. Infected Feldmannia plants show severe somatic malformations. However, no functional virus particles are formed. Such Feldmannia plants recover to resume a normal, symptom-free appearance. This result raises the possibility of intergeneric gene transfer in the natural habitat.	UNIV NACL SUR,DEPT BIOL,RA-8000 BAHIA BLANCA,ARGENTINA	MULLER, DG (corresponding author), UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY.						HENRY EC, 1992, J PHYCOL, V28, P517, DOI 10.1111/j.0022-3646.1992.00517.x; LANKA STJ, 1993, VIROLOGY, V193, P802, DOI 10.1006/viro.1993.1189; MULLER DG, 1992, HELGOLANDER MEERESUN, V46, P1, DOI 10.1007/BF02366208; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1992, NATURWISSENSCHAFTEN, V79, P37, DOI 10.1007/BF01132281; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; MULLER DG, 1993, IN PRESS HYDROBIOLOG; Starr R.C., 1987, Journal of Phycology, V23, P1	8	22	23	0	4	SPRINGER-VERLAG WIEN	VIENNA	SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA	0033-183X			PROTOPLASMA	Protoplasma		1993	175	3-4					121	125		10.1007/BF01385009			5	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	LZ891	WOS:A1993LZ89100005					2021-04-07	
J	KATSAROS, CI				KATSAROS, CI			IMMUNOFLUORESCENCE STUDY OF MICROTUBULE ORGANIZATION IN SOME POLARIZED CELL-TYPES OF SELECTED BROWN-ALGAE	BOTANICA ACTA			English	Article						BROWN ALGAE; POLARIZATION; MICROTUBULES; IMMUNOFLUORESCENCE; SPHACELARIA-RIGIDULA; ECTOCARPUS-SILICULOSUS	SILICULOSUS DILLW LYNGB; PHYSCOMITRELLA-PATENS; PYLAIELLA-LITTORALIS; THALLUS DEVELOPMENT; PHAEOPHYTA; REPRODUCTION; MICROSCOPY; MICROFILAMENTS; ULTRASTRUCTURE; CYTOKINESIS	The microtubule (Mt) organization in apical cells of Sphacelaria rigidula, as well as in branch initials of S. rigidula and Ectocarpus siliculosus, was studied by immunofluorescence. The apical interphase cells of S. rigidula show an impressive cytoskeleton of Mts, converging on the centrosome(s). A number of Mt bundles are perinuclear, but most of them run in axial orientation from the centrosomes to the cell cortex. The anterior Mt system consists of numerous thin Mt bundles, whereas the posterior system contains fewer and thicker bundles. In cells entering prophase, the cytoplasmic Mts gradually disappear. This process is somewhat faster at the posterior than at the anterior pole of the premitotic nucleus. After mitosis, the cytoplasmic Mts of the apical region appear to be re-organized more rapidly than those of the basal part of the cell. The apical daughter nucleus retains a lobed shape and condensed chromatin for a longer time, and increases considerably in size between telophase and cytokinesis, compared to the basal one. Duplication of the centrosomes proceeds more rapidly in the anterior region of apical cells than in the basal part. During branch formation in S. rigidula and E. siliculosus, a new polarity axis is established, and the Mts extend towards the protrusion into which the nucleus migrates before mitosis. After nuclear division, one of the daughter nuclei is positioned at the tip of the branch, where the apical Mt focussing point is localized.		KATSAROS, CI (corresponding author), UNIV ATHENS, INST GEN BOT, GR-15784 ATHENS, GREECE.						APOSTOLAKOS P, 1987, PROTOPLASMA, V140, P26, DOI 10.1007/BF01273253; Doonan J. H., 1988, ADV BRYOL, V3, P1; DOONAN JH, 1988, J CELL SCI, V89, P533; DOONAN JH, 1985, J CELL SCI, V75, P131; DOONAN JH, 1986, SOC EXPT BIOL SEMINA, V29, P111; GREEN PB, 1973, BROOKHAVEN SYM BIOL, P166; HALLAM ND, 1988, BRIT PHYCOL J, V23, P337, DOI 10.1080/00071618800650371; HOCH HC, 1985, PROTOPLASMA, V124, P112, DOI 10.1007/BF01279730; KATSAROS C, 1990, BRIT PHYCOL J, V25, P63, DOI 10.1080/00071619000650061; KATSAROS C, 1991, BOT ACTA, V104, P87, DOI 10.1111/j.1438-8677.1991.tb00201.x; KATSAROS C, 1992, PROTOPLASMA, V169, P75, DOI 10.1007/BF01343372; KATSAROS C, 1983, J PHYCOL, V19, P16, DOI 10.1111/j.0022-3646.1983.00016.x; KATSAROS C, 1985, BRIT PHYCOL J, V20, P263, DOI 10.1080/00071618500650271; KATSAROS C, 1988, BRIT PHYCOL J, V23, P71, DOI 10.1080/00071618800650091; KATSAROS C, 1980, THESIS ATHENS; LACLAIRE JW, 1982, PHYCOLOGIA, V21, P273, DOI 10.2216/i0031-8884-21-3-273.1; LLOYD CW, 1984, INT REV CYTOL, V86, P1, DOI 10.1016/S0074-7696(08)60176-X; LLOYD CW, 1985, J CELL SCI, P143; LLOYD CW, 1987, DEV BIOL COMPREHENSI, V2, P31; MARKEY DR, 1976, PROTOPLASMA, V88, P147, DOI 10.1007/BF01283243; MARKEY DR, 1975, PROTOPLASMA, V85, P219, DOI 10.1007/BF01567948; MOTOMURA T, 1985, Japanese Journal of Phycology, V33, P199; MOTOMURA T, 1991, J PHYCOL, V27, P248, DOI 10.1111/j.0022-3646.1991.00248.x; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; NEUSHUL M, 1972, AM J BOT, V59, P401, DOI 10.2307/2441551; PIERSON ES, 1986, EUR J CELL BIOL, V41, P14; RUNEBERG P, 1986, EUR J CELL BIOL, V41, P25; Sauvageau C., 1903, J BOT, V17, P45; SCHMIEDEL G, 1979, PROTOPLASMA, V101, P47, DOI 10.1007/BF01293434; SCHMIEDEL G, 1979, PROTOPLASMA, V100, P367, DOI 10.1007/BF01279323; SCHNEPF E, 1986, ANNU REV PLANT PHYS, V37, P23, DOI 10.1146/annurev.arplant.37.1.23; SCHNEPF E, 1988, CELL INTERACTIONS DI; STAEHELIN LA, 1982, DEV ORDER ITS ORIGIN, P133; STARR RC, 1978, J PHYCOL, V14, P47, DOI 10.1111/j.1529-8817.1978.tb02507.x	34	14	14	1	1	GEORG THIEME VERLAG KG	STUTTGART	RUDIGERSTR 14, D-70469 STUTTGART, GERMANY	0932-8629			BOT ACTA	Bot. Acta	DEC	1992	105	6					400	406		10.1111/j.1438-8677.1992.tb00320.x			7	Plant Sciences	Plant Sciences	KK221	WOS:A1992KK22100002					2021-04-07	
J	SCHMID, CE; SCHROER, N; KAWAI, H; MULLER, DG				SCHMID, CE; SCHROER, N; KAWAI, H; MULLER, DG			ISOLATION AND BIOCHEMICAL-CHARACTERIZATION OF DIFFERENT GAMETE MEMBRANES IN THE CHROMOPHYTE ALGA ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	PLANT PHYSIOLOGY AND BIOCHEMISTRY			English	Article						ATPASE; ENDOMEMBRANES; FINE STRUCTURE OF MEMBRANES; PLASMA MEMBRANE PURIFICATION; SUBCELLULAR FRACTIONATION	PLASMA-MEMBRANE; ADENOSINE-TRIPHOSPHATASE; CUTLERIA-MULTIFIDA; BROWN-ALGAE; PROTEINS; ATPASE; POLYACRYLAMIDE; VANADATE; GELS	Gametes of Ectocarpus siliculosus were used to develop an efficient method to separate die plasma membrane (PM) from the chloroplast endoplasmic reticulum (CER) including the bulk of endomembranes. Both fractions of PM and CER were characterized by their biochemical composition and by electron microscopy. Distribution of membrane markers and two-dimensional protein electrophoresis showed differences between PM- and CER-fractions. Ectocarpus PM-ATPase revealed conservative enzyme features such as molecular mass (1 12 kDa), vanadate inhibition (I50=0.7 muM), insensitivity to molybdate (0.1 mM) and azide (I mM), but also unusual characteristics such as a shift of maximum activity to low temperatures (T(max)=24-degrees-C) and a broad pH-optimum (pH 6.5-7.5). These properties are interpreted as adaptations to the natural habitat of Ectocarpus.	HOKKAIDO UNIV,FAC SCI,DEPT BOT,SAPPORO,HOKKAIDO 060,JAPAN	SCHMID, CE (corresponding author), UNIV CONSTANCE,FAK BIOL,POSTFACH 5560,W-7750 CONSTANCE,GERMANY.						BLAKE MS, 1984, ANAL BIOCHEM, V136, P175, DOI 10.1016/0003-2697(84)90320-8; BOLAND W, 1989, Z NATURFORSCH C, V44, P829; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLWELL GP, 1980, J CELL SCI, V43, P209; BRISKIN DP, 1987, METHOD ENZYMOL, V148, P542; CALLOW JA, 1985, J CELL SCI S, V2, P219; CALLOW ME, 1978, J CELL SCI, V32, P337; DIETRICH G, 1975, ALLGEMEINE MEERESKUN; Dodge JD, 1973, FINE STRUCTURE ALGAL; DUNBAR BS, 1987, 2 DIMENSIONAL ELECTR; EDWARDS P, 1973, CONTRIB MAR SCI, V17, P15; GALLAGHER SR, 1982, PLANT PHYSIOL, V70, P1335, DOI 10.1104/pp.70.5.1335; GIMMLER H, 1989, Z NATURFORSCH C, V44, P128; GRIFFING LR, 1984, P NATL ACAD SCI-BIOL, V81, P4804, DOI 10.1073/pnas.81.15.4804; HEUKESHOVEN J, 1985, ELECTROPHORESIS, V6, P103, DOI 10.1002/elps.1150060302; Hodges T K, 1974, Methods Enzymol, V32, P392; HODGES TK, 1986, METHOD ENZYMOL, V18, P41; JEFFREY SW, 1975, BIOCHEM PHYSIOL PFL, V167, P191, DOI 10.1016/s0015-3796(17)30778-3; KIM CK, 1980, PLANT CELL PHYSIOL, V21, P755, DOI 10.1093/oxfordjournals.pcp.a076050; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KYHSEANDERSEN J, 1984, J BIOCHEM BIOPH METH, V10, P203, DOI 10.1016/0165-022X(84)90040-X; LABARCA C, 1980, ANAL BIOCHEM, V102, P344, DOI 10.1016/0003-2697(80)90165-7; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; Larsson C., 1990, The plant plasma membrane., P1; LEONARD RT, 1988, PLANT MEMBRANES, P179; LOFTHOUSE PF, 1975, PROTOPLASMA, V84, P83, DOI 10.1007/BF02075945; LUNING K, 1985, MEERESBOTANIK; MacKinney G, 1941, J BIOL CHEM, V140, P315; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; PETERSON GL, 1977, ANAL BIOCHEM, V83, P346, DOI 10.1016/0003-2697(77)90043-4; PRICE A, 1974, METHOD ENZYMOL, V31, P501; RAGAN MA, 1977, J EXP MAR BIOL ECOL, V30, P209, DOI 10.1016/0022-0981(77)90013-2; ROBINSON DG, 1985, PLANT MEMBRANES; SCHIBECI A, 1985, P37; SCHNEIDER WC, 1945, J BIOL CHEM, V161, P293; South G. R., 1987, INTRO PHYCOLOGY; SULLIVAN CW, 1975, ARCH BIOCHEM BIOPHYS, V167, P437, DOI 10.1016/0003-9861(75)90484-1; SZE H, 1985, ANNU REV PLANT PHYS, V36, P175, DOI 10.1146/annurev.pp.36.060185.001135; UEMURA M, 1984, PLANT PHYSIOL, V75, P818, DOI 10.1104/pp.75.3.818; WEISS M, 1989, BIOCHIM BIOPHYS ACTA, V974, P254, DOI 10.1016/S0005-2728(89)80241-5	44	4	4	0	1	GAUTHIER-VILLARS	PARIS	S P E S-JOURNAL DEPT, 120 BD ST GERMAIN, F-75006 PARIS, FRANCE	0981-9428			PLANT PHYSIOL BIOCH	Plant Physiol. Biochem.	NOV-DEC	1992	30	6					703	712					10	Plant Sciences	Plant Sciences	KH332	WOS:A1992KH33200010					2021-04-07	
J	STRATMANN, K; BOLAND, W; MULLER, DG				STRATMANN, K; BOLAND, W; MULLER, DG			PHEROMONES OF MARINE BROWN-ALGAE - A NEW BRANCH OF THE EICOSANOID METABOLISM	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYCEAE; GIFFORDIA-MITCHELLAE; SENECIO-ISATIDEUS; BIOSYNTHESIS; HYDROCARBONS; MODEL; ACID	The pool Of polysaturated eicosanoids is used by female gametes of marine brown algae in the biosynthesis of their pheromones ectocarpene (A) and dictyotene (B). Higher plants synthesize these compounds from multiply unsaturated C-12 fatty acids.	UNIV KARLSRUHE,INST ORGAN CHEM,RICHARD WILLSTATTER ALLEE 2,W-7500 KARLSRUHE,GERMANY; UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY			Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			ABRAHAM WD, 1991, J AM CHEM SOC, V113, P2313, DOI 10.1021/ja00006a066; AKHTAR M, 1965, J CHEM SOC, P5964, DOI 10.1039/jr9650005964; BOLAND W, 1985, EUR J BIOCHEM, V147, P83, DOI 10.1111/j.1432-1033.1985.tb08722.x; BOLAND W, 1987, EXPERIENTIA, V43, P466, DOI 10.1007/BF01940458; BOLAND W, 1987, HELV CHIM ACTA, V70, P1025, DOI 10.1002/hlca.19870700415; Boland W, 1984, ANAL VOLATILES, P371; BOLAND W, 1987, TETRAHEDRON LETT, P307; BOLAND W, 1987, BIOL UNSERER ZEIT, V17, P176; JAENICKE L, 1974, J AM CHEM SOC, V96, P3324, DOI 10.1021/ja00817a056; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MOORE RE, 1977, ACCOUNTS CHEM RES, V10, P40, DOI 10.1021/ar50110a002; MULLER DG, 1988, BIOL CHEM H-S, V369, P647, DOI 10.1515/bchm3.1988.369.2.647; MULLER DG, 1985, Z NATURFORSCH C, V40, P457; NAF F, 1975, HELV CHIM ACTA, V58, P1016, DOI 10.1002/hlca.19750580406; NEUMANN C, 1990, EUR J BIOCHEM, V191, P453, DOI 10.1111/j.1432-1033.1990.tb19143.x; SCHMID CE, 1991, BIOL CHEM H-S, V372, P540; WURZENBERGER M, 1984, BIOCHIM BIOPHYS ACTA, V795, P163, DOI 10.1016/0005-2760(84)90117-6; YAMADA K, 1986, TETRAHEDRON, V42, P3775, DOI 10.1016/S0040-4020(01)87531-1	18	29	29	0	14	VCH PUBLISHERS INC	DEERFIELD BEACH	303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788	0570-0833			ANGEW CHEM INT EDIT	Angew. Chem.-Int. Edit. Engl.	SEP	1992	31	9					1246	1248		10.1002/anie.199212461			3	Chemistry, Multidisciplinary	Chemistry	JR575	WOS:A1992JR57500037					2021-04-07	
J	FRIEDLANDER, M				FRIEDLANDER, M			GRACILARIA-CONFERTA AND ITS EPIPHYTES - THE EFFECT OF CULTURE CONDITIONS ON GROWTH	BOTANICA MARINA			English	Article							ULVA-LACTUCA; PHOTOSYNTHESIS	Growth rate experiments for Gracilaria conferta and its four competing main epiphytes were conducted in a growth chamber and on a growth gradient table under various growth conditions. The range of conditions tested was temperature (10 - 30-degrees-C), irradiance (47 - 404 muE m-2 s-1), salinity (10 - 50 ppt), pH (7.0 - 9. 0) and green versus white light. The conditions under which the growth of Gracilaria conferta was promoted while that of the epiphytes was limited were formulated as follows: Optimal temperature for Gracilaria conferta (25-degrees-C) was inhibiting for Cladophora pellucida. Optimal irradiance (172 muE m-2 S-1) for Gracilaria conferta was inhibitory for Ectocarpus confervoides. A pH of 7.0 partially excluded Enteromorpha compressa and Cladophora pellucida. Salinity of 20 ppt partially excluded Ulva lactuca and Ectocarpus confervoides. Enteromorpha compressa and Ulva lactuca were also selectively limited by green light. Interactions between Gracilaria and its epiphytes are discussed.		FRIEDLANDER, M (corresponding author), ISRAEL OCEANOG & LIMNOL RES LTD,NATL INST OCEANOG,POB 8030,IL-31080 HAIFA,ISRAEL.						DEBUSK TA, 1986, BOT MAR, V29, P381, DOI 10.1515/botm.1986.29.5.381; LUNING K, 1985, MAR BIOL, V87, P119, DOI 10.1007/BF00539419; RAMUS J, 1983, J PHYCOL, V19, P173, DOI 10.1111/j.0022-3646.1983.00173.x; REED RH, 1979, ESTUAR COAST MAR SCI, V8, P251, DOI 10.1016/0302-3524(79)90095-1; RYTHER JH, 1981, AQUACULTURE, V26, P107, DOI 10.1016/0044-8486(81)90114-9; THOMAS DN, 1988, BOT MAR, V31, P73, DOI 10.1515/botm.1988.31.1.73; VERMAAT JE, 1987, MAR BIOL, V95, P55, DOI 10.1007/BF00447485	7	35	38	0	5	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055			BOT MAR	Bot. Marina	SEP	1992	35	5					423	428		10.1515/botm.1992.35.5.423			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	JY245	WOS:A1992JY24500010					2021-04-07	
J	HENRY, EC; MEINTS, RH				HENRY, EC; MEINTS, RH			A PERSISTENT VIRUS-INFECTION IN FELDMANNIA (PHAEOPHYCEAE)	JOURNAL OF PHYCOLOGY			English	Article						DOUBLE-STRANDED DNA; FELDMANNIA; PHAEOPHYCEAE; SPORANGIA; ULTRASTRUCTURE; VIRUS	ALGA ECTOCARPUS; PARTICLES	A virus infection is described within the unilocular sporangia of Feldmannia sp., a filamentous brown alga (Phaeophyceae). The alga is easily maintained in culture and vegetative growth is vigorous, but formation of icosahedral virions 150 nm in diameter completely displaces production of zoospores. The viruses, estimated at 1-5 x 10(6) per sporangium, are eventually released by rupture of the sporangial wall. Deoxyribonucleic acid (DNA) isolated from the viruses can be readily digested with restriction endonucleases and consists of ca. 170 kbp of double-stranded DNA.		HENRY, EC (corresponding author), OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,CORVALLIS,OR 97331, USA.						BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BERKALOFF C, 1979, J PHYCOL, V15, P163, DOI 10.1111/j.0022-3646.1979.00163.x; CARDINAL A, 1964, BEIH NOVA HEDWIGIA, V15, P1; CLAYTON MN, 1974, AUST J BOT, V22, P743, DOI 10.1071/BT9740743; CLITHEROE SB, 1974, J ULTRA MOL STRUCT R, V49, P211, DOI 10.1016/S0022-5320(74)80032-8; FRANCKI RIB, 1991, ARCH VIROL S2, V19, P1; HENRY EC, 1983, PHYCOLOGIA, V22, P387, DOI 10.2216/i0031-8884-22-4-387.1; LECLAIRE JW, 1977, PROTOPLASMA, V93, P127; LINDAUER VW, 1961, NOVA HEDWIGIA, V3, P57; MARKEY D R, 1974, Protoplasma, V80, P223, DOI 10.1007/BF01666361; MARTIN EL, 1982, SELECTED PAPERS PHYC, P793; McLachlan J., 1973, HDB PHYCOLOGICAL MET, P25; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1972, PHYCOLOGIA, V11, P11; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; SAGA N, 1988, NOAA TECH REP NMSF, V70, P47; Sherman LA, 1978, COMPREHENSIVE VIROLO, P145; TOTH R, 1972, J PHYCOL, V8, P126, DOI 10.1111/j.1529-8817.1972.tb04011.x; TOTH R, 1974, J PHYCOL, V10, P170; VANETTEN JL, 1991, MICROBIOL REV, V55, P586, DOI 10.1128/MMBR.55.4.586-620.1991; VANETTEN JL, 1988, VIRUSES FUNGI SIMPLE, P411; Womersley H.B.S., 1987, MARINE BENTHIC FLO 2	23	36	36	0	3	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	AUG	1992	28	4					517	526		10.1111/j.0022-3646.1992.00517.x			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	JL278	WOS:A1992JL27800013					2021-04-07	
J	SCHMID, R; DRING, MJ				SCHMID, R; DRING, MJ			CIRCADIAN-RHYTHM AND FAST RESPONSES TO BLUE-LIGHT OF PHOTOSYNTHESIS IN ECTOCARPUS (PHAEOPHYTA, ECTOCARPALES) .1. CHARACTERIZATION OF THE RHYTHM AND THE BLUE-LIGHT RESPONSE	PLANTA			English	Article						ECTOCARPUS (PHAEOPHYTA); CIRCADIAN RHYTHM (PHOTOSYNTHESIS); BLUE LIGHT (STIMULATION OF PHOTOSYNTHESIS); PHASE SHIFTING (BLUE LIGHT); PHOTOSYNTHESIS	ASCOPHYLLUM-NODOSUM	The photosynthetic oxygen production of Ectocarpus siliculosus (Dillwyn) Lyngb. under continuous high irradiances of red light displayed a circadian rhythm with maxima at about noon. Pulses of blue light induced rapid transient increases in the rate of photosynthesis. The increases started about 15 s after the beginning of blue light and reached their maxima after 3-4 min. This was followed by a gradual decrease. A second peak or shoulder about 20 min later indicated that at least two reactions were involved in the blue-light enhancement of photosynthesis. The magnitude of the response to blue light depended on the phase of the rhythm at which blue light was given. It was high when the red-light photosynthesis was at its troughs, and low at its peaks. Fluence-response curves indicated that the sensitivity to blue light at the peaks of the rhythm was identical to that at the troughs. In addition, blue light shifted the phase of the photosynthetic rhythm, where the essential trigger was the light-off signal. Red light had no specific influence on the circadian rhythm. After darkness, photosynthetic rates were different from those under steady-state conditions. Two different transient bursts in the rate of O2 evolution could be distinguished, an early non-rhythmical one that was probably caused by accumulation of inorganic carbon inside the cells, and a second later one that appeared at the peak activity phases of the ciradian rhythm or after blue light. Its origins are unclear.	FREE UNIV BERLIN,INST PFLANZENPHYSIOL & MIKROBIOL,W-1000 BERLIN 33,GERMANY	SCHMID, R (corresponding author), QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND.		Dring, Matthew/B-4941-2014	Dring, Matthew/0000-0001-9043-5670			DRING MJ, 1989, J PHYCOL, V25, P254, DOI 10.1111/j.1529-8817.1989.tb00120.x; FORSTER R M, 1990, British Phycological Journal, V25, P87; JOHNSTON AM, 1986, J PHYCOL, V22, P78, DOI 10.1111/j.1529-8817.1986.tb02518.x; JOHNSTON AM, 1983, BRIT PHYCOL J, V18, P205; MULLER DIETER, 1962, BOT MARINA, V4, P140, DOI 10.1515/botm.1962.4.1-2.140; Ninnemann H., 1979, PHOTOCHEMICAL AND PHOTOBIOLOGICAL REVIEWS, V4, P207; SANCHO A, 1989, PLANT PHYSIOL BIOCH, V27, P537; SCHMID R, 1992, PLANTA, V187, P60, DOI 10.1007/BF00201624; SCHMID R, 1987, BLUE LIGHT RESPONSES, P87; Senger H., 1986, Photomorphogenesis in plants, P137; SEVERINGHAUS JW, 1958, J APPL PHYSIOL, V13, P515	11	30	30	0	14	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0032-0935			PLANTA	Planta	APR	1992	187	1					53	59		10.1007/BF00201623			7	Plant Sciences	Plant Sciences	HN023	WOS:A1992HN02300007	24177966				2021-04-07	
J	SCHMID, R; FORSTER, R; DRING, MJ				SCHMID, R; FORSTER, R; DRING, MJ			CIRCADIAN-RHYTHM AND FAST RESPONSES TO BLUE-LIGHT OF PHOTOSYNTHESIS IN ECTOCARPUS (PHAEOPHYTA, ECTOCARPALES) .2. LIGHT AND CO2 DEPENDENCE OF PHOTOSYNTHESIS	PLANTA			English	Article						ECTOCARPUS (PHAEOPHYTA); CIRCADIAN RHYTHM (PHOTOSYNTHESIS); BLUE LIGHT (STIMULATION OF PHOTOSYNTHESIS); PHOTOSYNTHESIS (IRRADIANCE AND CO2 DEPENDENCE OF RHYTHM)	INORGANIC CARBON-SOURCES; CHLAMYDOMONAS-SEGNIS; DIOXIDE	Photosynthesis of Ectocarpus siliculosus (Dillwyn) Lyngb. under continuous saturating red irradiation follows a circadian rhythm. Blue-light pulses rapidly stimulate photosynthesis with high effectiveness in the troughs of this rhythm but the effectiveness of such pulses is much lower at its peaks. In an attempt to understand how blue light and the rhythm affected photosynthesis, the effects of inorganic carbon on photosynthetic light saturation curves were studied under different irradiation conditions. The circadian rhythm of photosynthesis was apparent only at irradiances which were not limiting for photosynthesis. The same was found for blue-light-stimulated photosynthesis, although stimulation was observed also under very low red-light irradiances after a period of adaptation, provided that the inorganic-carbon concentration was not in excess. Double-reciprocal plots of light-saturated photosynthetic rates versus the concentration of total inorganic carbon (up to 10 mM total inorganic carbon) were linear and had a common constant for half-saturation (3.6 mM at pH 8) at both the troughs and the peaks of the rhythm and before and after blue-light pulses. Only at very low carbon concentrations was a clear deviation found from these lines for photosynthesis at the rhythm maxima (red and blue light), which indicated that the strong carbon limitation specifically affected photosynthesis at the peak phases of the rhythm. Very high inorganic carbon concentrations (20 mM) in the medium diminished the responses to blue light, although they did not fully abolish them. The kinetics of the stimulation indicate that the rate of photosynthesis is affected by two blue-light-dependent components with different time courses of induction and decay. The faster component seemed to be at least partially suppressed at red-light irradiances which were not saturating for photosynthesis. Lowering the pH of the medium had the same effects as an increase of the carbon concentration to levels of approx. 10 mM. This indicates that Ectocarpus takes up free CO2 only and not bicarbonate, although additional physiological mechanisms may enhance the availability of CO2.	FREE UNIV BERLIN,INST PFLANZENPHYSIOL & MIKROBIOL,W-1000 BERLIN 33,GERMANY	SCHMID, R (corresponding author), QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND.		Dring, Matthew/B-4941-2014; Forster, Rodney/J-1756-2019	Dring, Matthew/0000-0001-9043-5670; Forster, Rodney/0000-0001-6990-1673			AXELSSON L, 1988, MAR BIOL, V97, P295, DOI 10.1007/BF00391315; BADOUR SS, 1990, BOT ACTA, V103, P149, DOI 10.1111/j.1438-8677.1990.tb00141.x; Blackman FF, 1905, ANN BOT-LONDON, V19, P281, DOI 10.1093/oxfordjournals.aob.a089000; BURGER J, 1988, BOT ACTA, V101, P229, DOI 10.1111/j.1438-8677.1988.tb00037.x; FORSTER R, 1991, IN PRESS PLANT CELL; GERGIS MS, 1972, ARCH MIKROBIOL, V83, P321, DOI 10.1007/BF00425244; MARCUS Y, 1986, PLANT PHYSIOL, V80, P604, DOI 10.1104/pp.80.2.604; MULLER DIETER, 1962, BOT MARINA, V4, P140, DOI 10.1515/botm.1962.4.1-2.140; RAVEN JA, 1970, BIOL REV, V45, P167, DOI 10.1111/j.1469-185X.1970.tb01629.x; RAVEN JA, 1985, PLANT CELL ENVIRON, V8, P417, DOI 10.1111/j.1365-3040.1985.tb01677.x; RAVEN JA, 1990, BR PHYCOL J, V25, P75; SCHMID R, 1991, PLANTA, V187, P53; SPALDING MH, 1984, PHOTOSYNTH RES, V5, P169, DOI 10.1007/BF00028529; SURIF MB, 1989, OECOLOGIA, V78, P97, DOI 10.1007/BF00377203; TAN CK, 1983, Z PFLANZENPHYSIOL, V109, P113, DOI 10.1016/S0044-328X(83)80201-3; TSUZUKI M, 1989, AQUAT BOT, V34, P85, DOI 10.1016/0304-3770(89)90051-X	16	26	26	0	21	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0032-0935			PLANTA	Planta	APR	1992	187	1					60	66		10.1007/BF00201624			7	Plant Sciences	Plant Sciences	HN023	WOS:A1992HN02300008	24177967				2021-04-07	
J	BEGUM, M; KHATOON, N				BEGUM, M; KHATOON, N			CONTRIBUTION TO THE TAXONOMY OF THE ECTOCARPALES (PHAEOPHYCEAE) FROM THE COAST OF KARACHI, PAKISTAN	BOTANICAL JOURNAL OF THE LINNEAN SOCIETY			English	Article						ALGAE; ECTOCARPUS; GIFFORDIA; HINCKSIA; NEMACYSTUS	SEAWEEDS; LASBELA			BEGUM, M (corresponding author), UNIV KARACHI, DEPT BOT, KARACHI 32, PAKISTAN.						Abbott I. A., 1976, MARINE ALGAE CALIFOR; Anand P.L., 1943, MARINE ALGAE KARAC 2; ANAND PL, 1940, MARINE ALGAE KARAC 1; BATTERS E.A.L., 1893, GREVILLEA, V21, P85; BEGUM M, 1988, PAKISTAN J BOT, V20, P291; BOERGESEN F., 1932, JOUR INDIAN BOT SOC, V11, P51; BOERGESEN F, 1949, DANSKE VIDENSKABERNE, V21, P1; Boergesen F., 1934, DANSKE VIDENSKABERNE, V11, P1; BOERGESEN F, 1948, DANSKE VIDENSKABERNE, V20, P1; BOERGESEN F, 1941, DANSKE VIDENSKABERNE, V14, P1; BOERGESEN F, 1935, DANSK VIDENSK SELSK, V12, P1; BOERGESEN F, 1953, DANSKE VIDENSKABERNE, V21, P1; BOERGESEN F, 1939, DANISH SCI INVESTIGA; BOERGESEN F, 1914, MARINE ALGAE DANISH, V1; DAWSON E. YALE, 1954, PACIFIC SCI, V8, P373; DAWSON EY, 1956, PAC SCI, V10, P25; Gray J. E., 1864, HDB BRIT WATER WEEDS; HAMEL G, 1939, BOT NOTISER, V1, P65; HARVEY WH, 1941, MANUAL BRIT ALGAE, V1; HARVEY WH, 1852, SMITHSONIAN CONTRIBU, V3, P150; Misra J N, 1966, PHAEOPHYCEAE INDIA; NASR AH, 1847, B FS, V26; NEWTON L, 1931, HDB BRFIT SEAWEEDS; NIZAMUDD.M, 1968, BOT MAR, V11, P40, DOI 10.1515/botm.1968.11.1-4.40; NIZAMUDDIN M, 1967, BOT MAR, V10, P158, DOI 10.1515/botm.1967.10.1-2.158; NIZAMUDDIN M., 1963, PACIFIC SCI, V17, P243; NIZAMUDDIN M, 1973, BOT MAR, V16, P1, DOI 10.1515/botm.1973.16.1.1; NIZAMUDDIN M, 1967, BOT MAR, V10, P169, DOI 10.1515/botm.1967.10.1-2.169; NIZAMUDDIN M, 1986, PAKISTAN J BOT, V18, P123; NIZAMUDDIN M, 1978, Revue Algologique, V13, P315; NIZAMUDDIN M, 1963, ANN BOTANY, V28, P77; NIZAMUDDIN MOHAMMED, 1964, BOT MAR, V6, P204, DOI 10.1515/botm.1964.6.3-4.204; PARKE M, 1953, J MAR BIOL ASSOC UK, V32, P503; RICHARDSON WD, 1975, B BRIT MUSEUM NATURA, V5; SAIFULLAH SM, 1977, BOT MAR, V20, P521, DOI 10.1515/botm.1977.20.8.521; SHAMEEL M, 1989, BOT MAR, V32, P177, DOI 10.1515/botm.1989.32.2.177; SHAMEEL M, 1987, BOT MAR, V30, P511, DOI 10.1515/botm.1987.30.6.511; Shameel M., 1972, NOVA HEDWIGIA, V23, P433; SILVA PC, 1987, CATALOG BENTHIC MARI, V27; Smith G. M, 1951, MARINE ALGAE MONTERE; SMITH JE, 1811, ENGLISH BOTANY, V33; Vickers A., 1905, ANN SCI NATURELLES 9, V1, P45; WOMERSLEY HBS, 1987, MAIRNE BENTHIC FLORA; ZAHID PB, 1981, PAKISTAN J BOT, V13, P195	44	3	7	0	1	WILEY-BLACKWELL	MALDEN	COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA	0024-4074			BOT J LINN SOC	Bot. J. Linnean Soc.	MAR	1992	108	3					239	252		10.1111/j.1095-8339.1992.tb00241.x			14	Plant Sciences	Plant Sciences	HR528	WOS:A1992HR52800003					2021-04-07	
J	KAWAI, H				KAWAI, H			GREEN FLAGELLAR AUTOFLUORESCENCE IN BROWN ALGAL SWARMERS AND THEIR PHOTOTACTIC RESPONSES	BOTANICAL MAGAZINE-TOKYO			English	Article						ACTION SPECTRUM; BROWN ALGAE; CHROMOPHYTA; FLAGELLAR AUTOFLUORESCENCE; FLAGELLAR SWELLING; PHOTOTAXIS; STIGMA	LASER SCANNING MICROSCOPY; EUGLENA-GRACILIS; ECTOCARPUS-SILICULOSUS; PARAFLAGELLAR BODY; ACTION SPECTRA; PRYMNESIOPHYCEAE; DINOFLAGELLATE; ULTRASTRUCTURE; FLUORESCENCE; APPARATUS	A flavin-like green autofluorescent substance is noticed to occur in one of the flagella of flagellated cells in the Phaeophyceae, Chrysophyceae, Synurophyceae, Xanthophyceae and Prymnesiophyceae. In the phaeophycean swarmers the autofluorescence occurs in the posterior flagellum throughout its length. It is considered to be involved in the photoreception of phototaxis, since it almost always occurs in the swarmers which have a flagellar swelling and stigma and show phototaxis. In the phaeophycean swarmers, the stigma is shown to act as a concave reflector mirror focusing the reflection light onto the flagellar swelling. In the action spectrum studies, phaeophycean swarmers showed phototaxis between 370 and 520 nm, having two major peaks at 420 or 430 nm and 450 or 460 nm. Their responses were true phototactic and not photophobic. Rotation of the swarmer was shown to be essential in the photoreception of Ectocarpus gametes.	HOKKAIDO UNIV,FAC SCI,DEPT BOT,SAPPORO,HOKKAIDO 060,JAPAN							ANDERSEN RA, 1987, AM J BOT, V74, P337, DOI 10.2307/2443810; ANDERSEN RA, 1985, PROTOPLASMA, V128, P94, DOI 10.1007/BF01276332; BELCHER J. H., 1967, BRIT PHYCOL BULL, V3, P257; BENEDETTI PA, 1975, PLANT SCI LETT, V4, P47, DOI 10.1016/0304-4211(75)90074-7; BENEDETTI PA, 1977, PHOTOCHEM PHOTOBIOL, V26, P315, DOI 10.1111/j.1751-1097.1977.tb07492.x; Bold H. C., 1985, INTRO ALGAE; Cavalier-Smith T., 1986, Progress phycol. Res., V4, P309; CAVALIERSMITH T, 1989, CHROMOPHYTE ALGAE PR, P381; CLAYTON MN, 1989, CHROMOPHYTE ALGAE, P229; COLEMAN AW, 1988, J PHYCOL, V24, P118; DIEHN B, 1969, BIOCHIM BIOPHYS ACTA, V177, P136, DOI 10.1016/0304-4165(69)90073-7; Dodge JD, 1973, FINE STRUCTURE ALGAL; FORWARD RB, 1973, PLANTA, V111, P167, DOI 10.1007/BF00386277; FORWARD RB, 1974, J PROTOZOOL, V21, P312, DOI 10.1111/j.1550-7408.1974.tb03659.x; FOSTER KW, 1980, MICROBIOL REV, V44, P572, DOI 10.1128/MMBR.44.4.572-630.1980; GREEN JC, 1977, J MAR BIOL ASSOC UK, V57, P1125, DOI 10.1017/S0025315400026175; GREEN JC, 1980, BRIT PHYCOL J, V15, P151, DOI 10.1080/00071618000650171; HADER DP, 1986, CURR MICROBIOL, V14, P157, DOI 10.1007/BF01568368; HALLDAL P, 1961, PHYSIOL PLANTARUM, V14, P133, DOI 10.1111/j.1399-3054.1961.tb08146.x; HALLDAL PER, 1958, PHYSIOL PLANTARUM, V11, P118; HENRY EC, 1982, J PHYCOL, V8, P550; HIBBERD DJ, 1972, ANN BOT-LONDON, V36, P49, DOI 10.1093/oxfordjournals.aob.a084577; INOUYE I, 1990, Japanese Journal of Phycology, V38, P11; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; KAWAI H, 1991, PROTOPLASMA, V161, P17, DOI 10.1007/BF01328893; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KAWAI H, 1988, J PHYCOL, V24, P114; KREIMER G, 1991, J PHYCOL, V27, P268, DOI 10.1111/j.0022-3646.1991.00268.x; KREIMER G, 1990, EUR J CELL BIOL, V53, P101; Kristiansen J., 1990, P438; KRISTIANSEN J, 1976, PROTOPLASMA, V89, P371, DOI 10.1007/BF01275753; KRISTIANSEN J, 1977, BRIT PHYCOL J, V12, P329, DOI 10.1080/00071617700650351; Manton I, 1966, J ROY MICROSC SOC, V85, P119, DOI 10.1111/j.1365-2818.1966.tb02174.x; MAST SO, 1927, ARCH PROTISTENKD, V60, P197; MAST SO, 1911, LIGHT BEHAVIOR ORG; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; NULTSCH W, 1971, ARCH MIKROBIOL, V80, P351, DOI 10.1007/BF00406222; ROUILLER C, 1958, EXP CELL RES, V14, P47, DOI 10.1016/0014-4827(58)90212-X; SCHMIDT W, 1990, PLANTA, V182, P375, DOI 10.1007/BF02411388; SOUGH GR, 1987, INTRO PHYCOLOGY; WATANABE M, 1974, PLANT CELL PHYSIOL, V15, P413	42	19	19	0	6	BOTANICAL SOC JAPAN	TOKYO	TOSHIN BUILDING HONGO 2-27-2 BUNKYO-KU, TOKYO 113, JAPAN	0006-808X			BOT MAG TOKYO		MAR	1992	105	1077					171	184		10.1007/BF02489413			14	Plant Sciences	Plant Sciences	KN527	WOS:A1992KN52700015					2021-04-07	
J	MULLER, DG; STACHE, B				MULLER, DG; STACHE, B			WORLDWIDE OCCURRENCE OF VIRUS-INFECTIONS IN FILAMENTOUS MARINE BROWN-ALGAE	HELGOLANDER MEERESUNTERSUCHUNGEN			English	Article							EVOLUTION	Virus infections were detected in Ectocarpus siliculosus and Ectocarpus fasciculatus on the coasts of Ireland, California, Peru, southern South America, Australia and New Zealand; in three Feldmannia species on the coasts of Ireland, continental Chile and Archipelago Juan Fernandez (Chile); and in Leptonematella from Antarctica. Natural populations on the Irish coast contained 3 % infected plants in E. fasciculatus, and less than 1 % in Feldmannia simplex. On the Californian coast, 15 to 25 % of Ectocarpus isolates were infected. Virus symptoms were absent in E. siliculosus from Peru, but appeared after meiosis in laboratory cultures. The virus particles in E. fasciculatus are identical in size and capsid structure to those reported for E. siliculosus, while the virus in F. simplex is smaller and has a different envelope. Our findings suggest that virus infections are a common and worldwide phenomenon in filamentous brown algae.		MULLER, DG (corresponding author), UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY.						ANDREWS JH, 1976, BIOL REV, V51, P211, DOI 10.1111/j.1469-185X.1976.tb01125.x; ARDRE F, 1969, ACTA BIOLOGICA, V10, P137; Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; Jaasund E, 1965, BOT GOTHOBURG, V4, P1; MULLER DG, 1990, BOT ACTA, V103, P72; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1991, J PLANT PHYSIOL, V137, P739; MULLER DG, 1991, MAR ECOL PROG SER, V76, P101, DOI 10.3354/meps076101; REANNEY DC, 1974, INT REV CYTOL, V37, P21, DOI 10.1016/S0074-7696(08)61356-X; Starr R.C., 1987, Journal of Phycology, V23, P1; TOTH R, 1972, J PHYCOL, V8, P126, DOI 10.1111/j.1529-8817.1972.tb04011.x; USHIYAMA R, 1985, MICROBIOL SCI, V2, P181	12	33	33	0	4	BIOLOGISCHE ANSTALT HELGOLAND	HAMBURG	NOTKESTRASSE 31, 22607 HAMBURG, GERMANY	0174-3597			HELGOLANDER MEERESUN	Helgol. Meeresunters.		1992	46	1					1	8		10.1007/BF02366208			8	Marine & Freshwater Biology; Oceanography	Marine & Freshwater Biology; Oceanography	HW910	WOS:A1992HW91000001		Bronze			2021-04-07	
J	THOMAS, DN; KIRST, GO				THOMAS, DN; KIRST, GO			DIFFERENCES IN OSMOACCLIMATION BETWEEN SPOROPHYTES AND GAMETOPHYTES OF THE BROWN ALGA ECTOCARPUS-SILICULOSUS	PHYSIOLOGIA PLANTARUM			English	Article							INTESTINALIS L LINK; LANOSA L TANDY; ROTH C AG; ENTEROMORPHA-INTESTINALIS; ESTUARINE SITES; OSMOTIC RESPONSES; MARINE-ALGAE; CELL-WALLS; PHAEOPHYTA; SALINITY	The osmoacclimation of Ectocarpus siliculosus isolates known to have different salt tolerances was investigated. Included were isolates originating from 5 different locations in the northern hemisphere, and sporophyte and gametophyte phases of different ploidy from two of the locations were compared. The effect of salinity treatment (8-64 parts per thousand) on inorganic ions (K+, Na+, Mg2+, Cl-, SO42-, phosphate) and the low molecular weight carbohydrate mannitol was measured, together with complimentary measurements of cell viability. Very different responses between isolates were obtained, both between isolates of different geographic origin and between sporophytes and gametophytes from the same parent material. A similarity in response between haploid and diploid gametophytes, and diploid and triploid sporophytes indicates that physiological differences between gametophyte and sporophyte generations are not necessarily based on ploidy changes alone. There were no identifiable differences in the responses of male and female gametophytes. K+ is the major osmolyte within the species, and differences in the regulation of K+ largely account for the observed variation in osmoacclimation, both between life history phases and between isolates from different localities. Isolates with broader salt tolerances had the higher concentrations of mannitol. There were differences between isolates in the amounts and regulation of Cl- and phosphate, the latter being present in unusually high concentrations. There were also isolate differences in the concentrations of Mg2+ and SO42-, although these divalent ions were present only in low concentrations.	UNIV BREMEN,DEPT MARINE BOT FB2,W-2800 BREMEN 33,GERMANY	THOMAS, DN (corresponding author), ALFRED WEGENER INST POLAR & MARINE RES,AM HANDELSHAFEN 12,W-2850 BREMERHAVEN,GERMANY.		Thomas, David N./B-1448-2010	Thomas, David N./0000-0001-8832-5907			BOLTON JJ, 1982, MAR BIOL, V66, P89, DOI 10.1007/BF00397259; CLAYTON MN, 1988, BOT MAR, V31, P379, DOI 10.1515/botm.1988.31.5.379; DAVISON IR, 1985, J PHYCOL, V21, P41; DICKSON DMJ, 1986, PLANTA, V167, P536, DOI 10.1007/BF00391230; EDWARDS DM, 1988, MAR BIOL, V98, P467, DOI 10.1007/BF00391537; GERLACH E, 1963, BIOCHEM Z, V337, P477; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; HANNACH G, 1985, MAR ECOL PROG SER, V22, P291, DOI 10.3354/meps022291; KARSTEN U, 1989, BOT ACTA, V102, P123; KARSTEN U, 1991, IN PRESS PLANT PHYSL, V29; KIRST GO, 1990, ANNU REV PLANT PHYS, V41, P21, DOI 10.1146/annurev.pp.41.060190.000321; KIRST GO, 1979, AUST J PLANT PHYSIOL, V6, P539; LEE JA, 1988, J PHYCOL, V24, P181, DOI 10.1111/j.1529-8817.1988.tb04232.x; LITTLER MM, 1987, J PHYCOL, V23, P501, DOI 10.1111/j.1529-8817.1987.tb02538.x; MULLER DG, 1970, NATURWISSENSCHAFTEN, V57, P357; Raven JA, 1976, ENCYCLOPEDIA PLANT A, P129; REED RH, 1980, J EXP BOT, V31, P1521, DOI 10.1093/jxb/31.6.1521; REED RH, 1984, J EXP MAR BIOL ECOL, V76, P131, DOI 10.1016/0022-0981(84)90061-3; REED RH, 1985, PHYCOLOGIA, V24, P35, DOI 10.2216/i0031-8884-24-1-35.1; REED RH, 1981, J EXP BOT, V32, P347, DOI 10.1093/jxb/32.2.347; REED RH, 1983, J EXP MAR BIOL ECOL, V68, P169, DOI 10.1016/0022-0981(83)90158-2; REED RH, 1983, BOT MAR, V26, P409, DOI 10.1515/botm.1983.26.9.409; RITCHIE RJ, 1982, J EXP BOT, V33, P140, DOI 10.1093/jxb/33.1.140; RITCHIE RJ, 1982, J EXP BOT, V33, P125, DOI 10.1093/jxb/33.1.125; RITCHIE RJ, 1988, J PLANT PHYSIOL, V133, P183, DOI 10.1016/S0176-1617(88)80135-4; RUSSELL G, 1975, ESTUAR COAST MAR SCI, V3, P91, DOI 10.1016/0302-3524(75)90008-0; THOMAS DN, 1991, BOT ACTA, V104, P26, DOI 10.1111/j.1438-8677.1991.tb00190.x; THOMAS DN, 1990, ESTUAR COAST SHELF S, V30, P201, DOI 10.1016/0272-7714(90)90064-X; WIENCKE C, 1981, Z PFLANZENPHYSIOL, V103, P247, DOI 10.1016/S0044-328X(81)80157-2; WIENCKE C, 1989, MAR ECOL PROG SER, V54, P189, DOI 10.3354/meps054189; WRIGHT PJ, 1989, J EXP BOT, V40, P1347, DOI 10.1093/jxb/40.12.1347; YOUNG AJ, 1987, J EXP BOT, V38, P1309, DOI 10.1093/jxb/38.8.1309; YOUNG AJ, 1987, J EXP BOT, V38, P1298, DOI 10.1093/jxb/38.8.1298; YOUNG AJ, 1984, SYSTEMATICS ASS SPEC, V27, P343	34	9	9	0	10	MUNKSGAARD INT PUBL LTD	COPENHAGEN	35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK	0031-9317			PHYSIOL PLANTARUM	Physiol. Plant.	OCT	1991	83	2					281	289		10.1034/j.1399-3054.1991.830212.x			9	Plant Sciences	Plant Sciences	GM457	WOS:A1991GM45700012					2021-04-07	
J	KARSTEN, U; THOMAS, DN; WEYKAM, G; DANIEL, C; KIRST, GO				KARSTEN, U; THOMAS, DN; WEYKAM, G; DANIEL, C; KIRST, GO			A SIMPLE AND RAPID METHOD FOR EXTRACTION AND SEPARATION OF LOW-MOLECULAR-WEIGHT CARBOHYDRATES FROM MACROALGAE USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY	PLANT PHYSIOLOGY AND BIOCHEMISTRY			English	Article						ALGAL OSMOLYTES	FLUCTUATING ANTARCTIC DAYLENGTHS; LONG-TERM CULTURE; SUGAR ALCOHOLS; GREEN; ALGA; RED; ACCUMULATION; SEASONALITY; ESTERS	A method has been developed to measure the contents of low molecular weight carbohydrates in various marine macroalgae, using simple extraction procedures followed by high performance liquid chromatography (HPLC). Five algal species were investigated, representing a diverse range of algal types and morphologies (Bostrychia scorpioides, Desmarestia anceps, Ectocarpus siliculosus, Palmaria decipiens, Stictosiphonia arbuscula). Hot water and various ethanol extractions of these compounds were compared. The former was shown to be sufficient for complete extraction. Two types of HPLC column were used. Although operating conditions and carbohydrate separation were different, quantitatively both gave the same results. These techniques are simple, rapid and require no harmful substances for extraction or elution. They therefore greatly simplify the study of carbohydrates used as osmolytes and storage compounds by macroalgae.	ALFRED WEGENER INST POLAR & MARINE RES,W-2850 BREMERHAVEN,GERMANY	KARSTEN, U (corresponding author), UNIV BREMEN,DEPT MARINE BOT,FACHBEREICH 2,W-2800 BREMEN,GERMANY.		Thomas, David N./B-1448-2010	Thomas, David N./0000-0001-8832-5907			BLAKENEY AB, 1983, CARBOHYD RES, V113, P291, DOI 10.1016/0008-6215(83)88244-5; BOROWITZKA LJ, 1980, SCIENCE, V210, P650, DOI 10.1126/science.210.4470.650; EDWARDS DM, 1987, MAR BIOL, V95, P583, DOI 10.1007/BF00393102; EISENBER.F, 1971, CARBOHYD RES, V19, P135, DOI 10.1016/S0008-6215(00)80323-7; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; HOLLIGAN PM, 1971, NEW PHYTOL, V70, P271, DOI 10.1111/j.1469-8137.1971.tb02528.x; KARSTEN U, 1989, PLANT SCI, V61, P29, DOI 10.1016/0168-9452(89)90115-5; KERBY NW, 1989, J CHROMATOGR, V479, P353, DOI 10.1016/S0021-9673(01)83350-1; KIRST GO, 1990, ANNU REV PLANT PHYS, V41, P21, DOI 10.1146/annurev.pp.41.060190.000321; KREMER BP, 1980, BRIT PHYCOL J, V15, P399, DOI 10.1080/00071618000650401; LAKER MF, 1980, J CHROMATOGR, V184, P457, DOI 10.1016/S0021-9673(00)93874-3; LOESCHER WH, 1987, PHYSIOL PLANTARUM, V70, P553, DOI 10.1111/j.1399-3054.1987.tb02857.x; PERCIVAL E, 1979, BRIT PHYCOL J, V14, P103, DOI 10.1080/00071617900650121; REED RH, 1984, BRIT PHYCOL J, V19, P381, DOI 10.1080/00071618400650421; THOMAS DN, 1991, BOT ACTA, V104, P26, DOI 10.1111/j.1438-8677.1991.tb00190.x; WIENCKE C, 1990, POLAR BIOL, V10, P601, DOI 10.1007/BF00239371; WIENCKE C, 1990, POLAR BIOL, V10, P589, DOI 10.1007/BF00239370; WIENCKE C, 1988, SER CIENT INACH, V37, P23; WOOD PJ, 1975, CARBOHYD RES, V42, P1, DOI 10.1016/S0008-6215(00)84095-1; WRIGHT PJ, 1989, J EXP BOT, V40, P1347, DOI 10.1093/jxb/40.12.1347	20	64	64	1	21	GAUTHIER-VILLARS	PARIS	S P E S-JOURNAL DEPT, 120 BD ST GERMAIN, F-75006 PARIS, FRANCE	0981-9428			PLANT PHYSIOL BIOCH	Plant Physiol. Biochem.	JUL-AUG	1991	29	4					373	378					6	Plant Sciences	Plant Sciences	GK163	WOS:A1991GK16300011					2021-04-07	
J	KATSAROS, C; KREIMER, G; MELKONIAN, M				KATSAROS, C; KREIMER, G; MELKONIAN, M			LOCALIZATION OF TUBULIN AND A CENTRIN-HOMOLOG IN VEGETATIVE CELLS AND DEVELOPING GAMETANGIA OF ECTOCARPUS-SILICULOSUS (DILLW) LYNGB (PHAEOPHYCEAE, ECTOCARPALES) - A COMBINED IMMUNOFLUORESCENCE AND CONFOCAL LASER SCANNING MICROSCOPE STUDY	BOTANICA ACTA			English	Article						ECTOCARPUS-SILICULOSUS; PHAEOPHYCEAE; CYTOSKELETON; IMMUNOFLUORESCENCE; TUBULIN; CENTRIN; CONFOCAL LASER SCANNING MICROSCOPY	ALGA PYLAIELLA-LITTORALIS; GREEN-ALGAE; FLAGELLAR APPARATUS; ULTRASTRUCTURE; REPRODUCTION; CYTOSKELETON; INTERPHASE; VISUALIZATION; MICROTUBULES; CENTROSOME	The distribution of tubulin and centrin in vegetative cells and during gametogenesis of Ectocarpus siliculosus was studied by immunofluorescence. In interphase cells bundles of microtubules are focused on the centriolar region near the nuclear surface. Some of the bundles ensheath the nucleus while others traverse the cytoplasm in various directions, sometimes reaching the cell cortex. Evaluation of serial optical sections by confocal laser scanning microscopy (CLSM) revealed that the perinuclear and "cytoplasmic" microtubule bundles presumably constitute a single complex. In interphase cells centrin is localized as a single bright spot in the centriolar region. In dividing cells duplication and separation of the microtubular complex and the centrin spot takes place. In post-mitotic cells with two nuclei, the centrioles are located at opposite cell poles, short microtubule bundles emanate from them and partially encompass the nucleus. During gametogenesis a gradual transformation of the vegetative cytoskeleton to the gametic flagellar apparatus occurs.	UNIV COLOGNE,INST BOT,LEHRSTUHL 1,W-5000 COLOGNE 41,GERMANY	KATSAROS, C (corresponding author), UNIV ATHENS,INST GEN BOT,GR-15784 ATHENS,GREECE.						BARON AT, 1988, J CELL BIOL, V107, P2669, DOI 10.1083/jcb.107.6.2669; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; DAVIES JM, 1973, J MAR BIOL ASSOC UK, V53, P237, DOI 10.1017/S0025315400022232; DEMEY J, 1982, P NATL ACAD SCI-BIOL, V79, P1898; DOONAN JH, 1985, J CELL SCI, V75, P13; HALLAM ND, 1988, BRIT PHYCOL J, V23, P337, DOI 10.1080/00071618800650371; HOHFELD I, 1988, PROTOPLASMA, V147, P16, DOI 10.1007/BF01403874; KATSAROS C, 1990, BRIT PHYCOL J, V25, P63, DOI 10.1080/00071619000650061; KATSAROS C, 1983, J PHYCOL, V19, P16, DOI 10.1111/j.0022-3646.1983.00016.x; KATSAROS C, 1988, BRIT PHYCOL J, V23, P71, DOI 10.1080/00071618800650091; KATSAROS C, 1980, THESIS ATHENS; KREIMER G, 1990, EUR J CELL BIOL, V53, P101; LACLAIRE JW, 1987, PLANTA, V171, P30, DOI 10.1007/BF00395065; LACLAIRE JW, 1982, PHYCOLOGIA, V21, P273, DOI 10.2216/i0031-8884-21-3-273.1; LLOYD CW, 1987, ANNU REV PLANT PHYS, V38, P119; LLOYD CW, 1985, J CELL SCI S, V2, P143; MARKEY DR, 1976, PROTOPLASMA, V88, P147, DOI 10.1007/BF01283243; MARKEY DR, 1975, PROTOPLASMA, V85, P219, DOI 10.1007/BF01567948; MELKONIAN M, 1989, BOT ACTA, V102, P3, DOI 10.1111/j.1438-8677.1989.tb00059.x; MELKONIAN M, 1991, IN PRESS ALGAL CELL; MENZEL D, 1986, PROTOPLASMA, V134, P30, DOI 10.1007/BF01276373; MOTOMURA T, 1985, Japanese Journal of Phycology, V33, P199; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; NEUSHUL M, 1972, AM J BOT, V59, P401, DOI 10.2307/2441551; OSBORN M, 1978, CELL, V14, P477, DOI 10.1016/0092-8674(78)90234-9; SALISBURY JL, 1989, J PHYCOL, V25, P201; SALISBURY JL, 1984, J CELL BIOL, V99, P962, DOI 10.1083/jcb.99.3.962; SALISBURY JL, 1986, CELL MOTIL CYTOSKEL, V6, P193, DOI 10.1002/cm.970060218; SALISBURY JL, 1988, J CELL BIOL, V107, P635, DOI 10.1083/jcb.107.2.635; SCHULZE D, 1987, EUR J CELL BIOL, V45, P51; SEAGULL RW, 1989, CRIT REV PLANT SCI, V8, P131, DOI 10.1080/07352688909382273; STARR RC, 1978, J PHYCOL, V14, P47, DOI 10.1111/j.1529-8817.1978.tb02507.x; WHITE JG, 1987, J CELL BIOL, V105, P41, DOI 10.1083/jcb.105.1.41	33	21	21	0	4	GEORG THIEME VERLAG	STUTTGART	P O BOX 30 11 20, D-70451 STUTTGART, GERMANY	0932-8629			BOT ACTA	Bot. Acta	APR	1991	104	2					87	92		10.1111/j.1438-8677.1991.tb00201.x			6	Plant Sciences	Plant Sciences	FM629	WOS:A1991FM62900002					2021-04-07	
J	KREIMER, G; KAWAI, H; MULLER, DG; MELKONIAN, M				KREIMER, G; KAWAI, H; MULLER, DG; MELKONIAN, M			REFLECTIVE PROPERTIES OF THE STIGMA IN MALE GAMETES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE) STUDIED BY CONFOCAL LASER SCANNING MICROSCOPY	JOURNAL OF PHYCOLOGY			English	Article						CONFOCAL LASER SCANNING MICROSCOPY; ECTOCARPUS-SILICULOSUS; EYESPOT; FLAGELLAR SWELLING; PHAEOPHYTA; PHOTOTAXIS; STIGMA	ALGAE; AUTOFLUORESCENCE; CHLAMYDOMONAS; PHOTOMOVEMENT; FLAGELLUM; CELLS	The reflection properties of the stigma in male gametes of Ectocarpus siliculosus (Dillw.) Lyngbye were investigated using confocal laser scanning microscopy in the epireflection contrast mode. The complex reflection pattern obtained after optical xy (horizontal) and xz (vertical) sectioning was consistent with stigma ultrastructure as revealed by serial thin sections. The intensity and pattern of the reflection signal varied with the orientation of the cell/stigma to the incident laser light. Maximal reflection occurred only in approximately normal orientation of the stigma to the light source. Focusing of reflected light from an elongated concave depression of the stigma on the region of the flagellar swelling was observed in xy and xz sections of living and fixed gametes. The results indicate the importance of mechanisms (focusing) other than quarter-wave interference reflection in signal amplification by the eyespot of flagellate algae.	HOKKAIDO UNIV,FAC SCI,DEPT BOT,SAPPORO,HOKKAIDO 060,JAPAN; UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY	KREIMER, G (corresponding author), UNIV COLOGNE,LEHRSTUHL I,INST BOT,GYRHOFSTR 15,W-5000 COLOGNE 41,GERMANY.						COLEMAN AW, 1988, J PHYCOL, V24, P118; DODGE J D, 1969, British Phycological Journal, V4, P199; Dodge JD, 1973, FINE STRUCTURE ALGAL; FOSTER KW, 1980, MICROBIOL REV, V44, P572, DOI 10.1128/MMBR.44.4.572-630.1980; FOSTERS KW, 1989, ALGAE EXPT SYSTEMS P, V7, P215; GELLER A, 1981, J EXP BIOL, V92, P53; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; KAWAI H, 1990, PLANTA, V182, P292, DOI 10.1007/BF00197124; KAWAI H, 1988, J PHYCOL, V24, P114; KREIMER G, 1990, EUR J CELL BIOL, V53, P101; MAIER I, 1990, J EXP BOT, V41, P869, DOI 10.1093/jxb/41.7.869; MAST SO, 1927, ARCH PROTISTENKD, V60, P197; MELKONIAN M, 1980, J ULTRA MOL STRUCT R, V72, P90, DOI 10.1016/S0022-5320(80)90138-0; Melkonian M., 1984, Progress phycol. Res., V3, P193; MORELLAURENS NML, 1983, PHOTOCHEM PHOTOBIOL, V37, P189, DOI 10.1111/j.1751-1097.1983.tb04457.x; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; NULTSCH W, 1988, PHOTOCHEM PHOTOBIOL, V47, P837, DOI 10.1111/j.1751-1097.1988.tb01668.x; REIZE IB, 1989, BOT ACTA, V102, P145, DOI 10.1111/j.1438-8677.1989.tb00083.x; SHGEPPARD CJR, 1989, J MICROSC-OXFORD, V154, P237; SHOTTON DM, 1989, J CELL SCI, V94, P175; WALNE PL, 1967, PLANTA, V77, P325, DOI 10.1007/BF00389319	22	25	25	0	4	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.	APR	1991	27	2					268	276		10.1111/j.0022-3646.1991.00268.x			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	FH983	WOS:A1991FH98300015					2021-04-07	
J	MULLER, DG				MULLER, DG			MENDELIAN SEGREGATION OF A VIRUS GENOME DURING HOST MEIOSIS IN THE MARINE BROWN ALGA ECTOCARPUS-SILICULOSUS	JOURNAL OF PLANT PHYSIOLOGY			English	Article						ECTOCARPUS; MEIOSIS; PHAEOPHYCEAE; VERTICAL AND HORIZONTAL TRANSMISSION; VIRIOPLANKTON; VIRUS (DNA-); VIRUS (-MARINE)		Some isolates of Ectocarpus siliculosus from New Zealand are infested with a virus. It infects swimming zoids, and its genome is transmitted by mitosis to all cells of the growing host plant. Virus particles are formed in abnormal gametangia and sporangia. Occasionally, infected gametophytes can form functional gametes, although they contain the virus genome. Sexual fusion of a healthy female with a virus-infested male gamete resulted in a sporophyte that showed virus symptoms. Meiosis in this plant appeared normal and meiospores developed to families of gametophytes that were examined for expression of sex and virus symptoms. Individual meiosporangia gave rise to roughly equal numbers of healthy and infected game-tophytes. In most progeny families the virus symptoms segregated with either the male or female sex. These data suggest that the virus genome passes through the host's meiosis like an extra chromosome, or as a provirus linked to one of the host's autosomes.		MULLER, DG (corresponding author), UNIV CONSTANCE,FAK BIOL,W-7750 CONSTANCE,GERMANY.						COUCH HB, 1955, PHYTOPATHOLOGY, V45, P63; FROSHEIS.FI, 1974, PHYTOPATHOLOGY, V64, P102, DOI 10.1094/Phyto-64-102; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1990, BOT ACTA, V103, P72; Starr R.C., 1987, Journal of Phycology, V23, P1; USHIYAMA R, 1985, MICROBIOL SCI, V2, P181; Weiss RA, 1982, RNA TUMOR VIRUSES MO	7	58	59	0	6	GUSTAV FISCHER VERLAG	STUTTGART	WOLLGRASWEG 49, D-70599 STUTTGART, GERMANY	0176-1617			J PLANT PHYSIOL	J. Plant Physiol.	APR	1991	137	6					739	743					5	Plant Sciences	Plant Sciences	FM104	WOS:A1991FM10400018					2021-04-07	
J	PHILLIPS, JA; CLAYTON, MN				PHILLIPS, JA; CLAYTON, MN			BIFLAGELLATE SPERMATOZOIDS IN THE DICTYOTALES - THE STRUCTURE OF GAMETES AND GAMETANGIA IN ZONARIA-ANGUSTATA (DICTYOTALES, PHAEOPHYTA)	PHYCOLOGIA			English	Article							ECTOCARPUS-SILICULOSUS PHAEOPHYTA; BROWN-ALGAE; FLAGELLAR STRUCTURE; ULTRASTRUCTURE; ATTRACTANT	The spermatozoids of Zonaria angustata (Kutzing) Papenfuss (Phaeophyta) are biflagellate, and not uniflagellate as reported in other species of the Dictyotales. The anterior flagellum is 20-27-mu-m long and, in addition to mastigonemes and a terminal acronema, has a row of 23-33 spines distributed along its length. The posterior flagellum is very short, 1-1.5-mu-m long. Gametangial walls are not contiguous and sterile cortical cells occur between oogonia. The outer tier of the antheridia consists of sterile cells containing numerous physodes. It is argued that these characteristics of Z. angustata indicate that the order Dictyotales is more closely related to other brown algae than was previously thought, and the possibility of a phylogenetic relationship between the orders Dictyotales and Cutleriales is discussed.		PHILLIPS, JA (corresponding author), MONASH UNIV,DEPT ECOL & EVOLUT BIOL,CLAYTON,VIC 3168,AUSTRALIA.						ALLENDER B M, 1983, Brunonia, V6, P73, DOI 10.1071/BRU9830073; BOLAND W, 1983, HELV CHIM ACTA, V66, P1905, DOI 10.1002/hlca.19830660632; Bold H. C., 1985, INTRO ALGAE; BORGESEN F, 1926, KONGELIGE DANSKE VID, V6, P1; Bouck G.B., 1972, Advances cell mol Biol, V2, P237; CARAM B, 1975, CR ACAD SCI D NAT, V281, P1089; CLAYTON MN, 1989, CHROMOPHYTE ALGAE, P229; DERBES M, 1856, ANN SCI NATURELLES B, V5, P209; EVANS LV, 1972, NEW PHYTOL, V71, P1173, DOI 10.1111/j.1469-8137.1972.tb01995.x; FARRANT P A, 1988, Proceedings of the Linnean Society of New South Wales, V110, P369; FRITSCH FE, 1945, STRUCTURE REPRODUCTI, V2; GELLER A, 1981, J EXP BIOL, V92, P53; Haupt AW, 1932, AM J BOT, V19, P239, DOI 10.2307/2436336; HAY ME, 1988, ANNU REV ECOL SYST, V19, P111, DOI 10.1146/annurev.es.19.110188.000551; HEATH IB, 1972, J PHYCOL, V8, P51, DOI 10.1111/j.0022-3646.1972.00051.x; HIBBERD DJ, 1972, ANN BOT-LONDON, V36, P49, DOI 10.1093/oxfordjournals.aob.a084577; LIDDLE LB, 1969, J PHYCOL, V5, P4, DOI 10.1111/j.1529-8817.1969.tb02568.x; LIDDLE LB, 1968, J PHYCOL, V4, P298, DOI 10.1111/j.1529-8817.1968.tb04698.x; MAIER I, 1991, J EXP BOT, V41, P869; MANTON I, 1959, J EXP BOT, V10, P448, DOI 10.1093/jxb/10.3.448; MANTON I, 1953, J EXP BOT, V4, P319, DOI 10.1093/jxb/4.3.319; Manton I, 1966, J ROY MICROSC SOC, V85, P119, DOI 10.1111/j.1365-2818.1966.tb02174.x; MATHIESON ARTHUR C., 1966, NOVA HEDWIGIA, V12, P65; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1981, SCIENCE, V212, P1040, DOI 10.1126/science.212.4498.1040; NEUSHUL M, 1968, AM J BOT, V55, P1068, DOI 10.2307/2440474; Papenfuss GF, 1943, AM J BOT, V30, P463, DOI 10.2307/2437282; PHILLIPS JA, 1990, BRIT PHYCOL J, V25, P295, DOI 10.1080/00071619000650311; PHILLIPS JA, 1990, PHYCOLOGIA, V29, P367, DOI 10.2216/i0031-8884-29-3-367.1; PHILLIPS JA, 1988, BOT MAR, V31, P437, DOI 10.1515/botm.1988.31.5.437; RAMON E, 1965, 5TH P INT SEAW S, P183; Reinke J., 1878, NOVA ACTA ACADEMIAE, V40, P1; SAUVAGEAU C, 1897, J BOTANIQUE, V11, P86; Sauvageau C., 1905, B SOC SCI ARCACHON, V8, P66; STEINBERG PD, 1985, ECOL MONOGR, V55, P333, DOI 10.2307/1942581; THIVY FRANCESCA, 1959, JOUR MAR BIOL ASSOC INDIA, V1, P69; UBISCH G, 1931, PUBBL STAZ ZOOL NAPO, V11, P361; UBISCH G. v., 1928, BER DEUTSCH BOT GES, V46, P457; Williams JL, 1904, ANN BOT-LONDON, V18, P183, DOI 10.1093/oxfordjournals.aob.a088957; Williams JL, 1897, ANN BOTANY, V11, P545, DOI 10.1093/oxfordjournals.aob.a088670; Womersley H.B.S., 1987, MARINE BENTHIC FLO 2; Yamanouchi S, 1913, BOT GAZ, V56, P0001, DOI 10.1086/331104	44	17	17	0	1	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897	0031-8884			PHYCOLOGIA	Phycologia	MAR	1991	30	2					205	214		10.2216/i0031-8884-30-2-205.1			10	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	FD574	WOS:A1991FD57400009					2021-04-07	
J	VISVIKI, I; RACHLIN, JW				VISVIKI, I; RACHLIN, JW			THE TOXIC ACTION AND INTERACTIONS OF COPPER AND CADMIUM TO THE MARINE ALGA DUNALIELLA-MINUTA, IN BOTH ACUTE AND CHRONIC EXPOSURE	ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY			English	Article							HEAVY-METAL TOLERANCE; ANABAENA-FLOS-AQUAE; ECTOCARPUS-SILICULOSUS; FOULING ALGA; MORPHOMETRIC ANALYSIS; GREEN-ALGA; ZINC; GROWTH; PHYTOPLANKTON; LEAD	The effective concentrations of copper and cadmium which reduced the population growth of Dunaliella minuta by 50% after 96 h of static exposure, were determined to be 7.57-mu-M Cu and 0.34-mu-M Cd. Short-term static exposure to both metals indicated that their combined action is antagonistic with respect to growth of this chlorophyte. Additionally, long-term exposure to low levels of Cu or Cd led to the acquisition of tolerance towards Cu and Cd, respectively, and co-tolerance towards Cu.		VISVIKI, I (corresponding author), CUNY HERBERT H LEHMAN COLL, DEPT BIOL SCI, BEDFORD PK BLVD W, BRONX, NY 10468 USA.				DRS NIH HHS [GRS 3SO 6 RRO 8225/05 S2/442-664] Funding Source: Medline		BARIAUD A, 1984, B ENVIRON CONTAM TOX, V32, P597, DOI 10.1007/BF01607543; BARTLETT L, 1974, WATER RES, V8, P179, DOI 10.1016/0043-1354(74)90041-4; BENTLEYMOWAT JA, 1977, J EXP MAR BIOL ECOL, V26, P249, DOI 10.1016/0022-0981(77)90085-5; BERK SG, 1981, J EXP MAR BIOL ECOL, V52, P157, DOI 10.1016/0022-0981(81)90034-4; BERLAND BR, 1977, MAR BIOL, V50, P239; BRAEK GS, 1980, J EXP MAR BIOL ECOL, V42, P39, DOI 10.1016/0022-0981(80)90165-3; BRAEK GS, 1976, J EXP MAR BIOL ECOL, V25, P37, DOI 10.1016/0022-0981(76)90074-5; BRETELER RJ, 1984, CHEM POLLUTION HUDSO, P12; BRYAN GW, 1971, PROC R SOC SER B-BIO, V177, P389, DOI 10.1098/rspb.1971.0037; DAVIES AG, 1976, J MAR BIOL ASSOC UK, V56, P39, DOI 10.1017/S0025315400020427; DAVIES AG, 1974, J MAR BIOL ASSOC UK, V54, P157, DOI 10.1017/S002531540002213X; Finney D.J., 1964, PROBIT ANAL; FINNEY DJ, 1964, STATISTICAL METHODS; FISHER NS, 1981, J EXP MAR BIOL ECOL, V51, P37, DOI 10.1016/0022-0981(81)90153-2; FISHER NS, 1981, J PHYCOL, V17, P108, DOI 10.1111/j.0022-3646.1981.00108.x; FOSTER PL, 1977, NATURE, V269, P322, DOI 10.1038/269322a0; FOSTER PL, 1982, LIMNOL OCEANOGR, V27, P745, DOI 10.4319/lo.1982.27.4.0745; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; HALL A, 1980, NEW PHYTOL, V85, P73, DOI 10.1111/j.1469-8137.1980.tb04449.x; HEUILLET E, 1986, BIOL CELL, V58, P79; JENSEN A, 1976, J EXP MAR BIOL ECOL, V22, P249, DOI 10.1016/0022-0981(76)90004-6; LUSTIGMAN BK, 1986, B ENVIRON CONTAM TOX, V37, P710, DOI 10.1007/BF01607828; MCKNIGHT DM, 1979, LIMNOL OCEANOGR, V24, P823, DOI 10.4319/lo.1979.24.5.0823; RACHLIN JW, 1983, B TORREY BOT CLUB, V110, P217, DOI 10.2307/2996345; RACHLIN JW, 1984, ARCH ENVIRON CON TOX, V13, P143, DOI 10.1007/BF01055871; RACHLIN JW, 1982, ARCH ENVIRON CON TOX, V11, P323; RACHLIN JW, 1985, ARCH ENVIRON CON TOX, V14, P395, DOI 10.1007/BF01055524; RACHLIN JW, 1982, ENV HLTH, V16, P145; RAI LC, 1981, BIOL REV, V56, P99, DOI 10.1111/j.1469-185X.1981.tb00345.x; RIVKIN RB, 1979, MAR BIOL, V50, P239, DOI 10.1007/BF00394205; ROSKO JJ, 1977, B TORREY BOT CLUB, V104, P226, DOI 10.2307/2484302; ROSKO JJ, 1975, B TORREY BOT CLUB, V102, P100, DOI 10.2307/2484731; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; SICKOGOAD L, 1979, J PHYCOL, V15, P316; SILVERBERG BA, 1977, ARCH ENVIRON CON TOX, V5, P305, DOI 10.1007/BF02220912; SILVERBERG BA, 1976, J CELL BIOL, V69, P210, DOI 10.1083/jcb.69.1.210; SORENTINO C, 1985, PHYCOLOGIA, V24, P366, DOI 10.2216/i0031-8884-24-3-366.1; STARR RC, 1978, J PHYCOL, V14, P47, DOI 10.1111/j.1529-8817.1978.tb02507.x; STOKES PM, 1975, VERH INT VER LIMNOL, V19, P2128; STRATTON GW, 1979, CHEMOSPHERE, V8, P731, DOI 10.1016/0045-6535(79)90034-1	40	49	54	0	5	SPRINGER	NEW YORK	233 SPRING ST, NEW YORK, NY 10013 USA	0090-4341	1432-0703		ARCH ENVIRON CON TOX	Arch. Environ. Contam. Toxicol.	FEB	1991	20	2					271	275		10.1007/BF01055915			5	Environmental Sciences; Toxicology	Environmental Sciences & Ecology; Toxicology	EW170	WOS:A1991EW17000017	2015003				2021-04-07	
J	THOMAS, DN; KIRST, GO				THOMAS, DN; KIRST, GO			SALT TOLERANCE OF ECTOCARPUS-SILICULOSUS (DILLW) LYNGB - COMPARISON OF GAMETOPHYTES, SPOROPHYTES AND ISOLATES OF DIFFERENT GEOGRAPHIC ORIGIN	BOTANICA ACTA			English	Article						ECTOCARPUS-SILICULOSUS; SALINITY STRESS; LIFE-CYCLE; POPULATION VARIATION; VIABILITY; PHOTOSYNTHESIS; RESPIRATION	LANOSA L TANDY; ESTUARINE SITES; RED ALGAE; TEMPERATURE TOLERANCE; OSMOTIC RESPONSES; LIFE HISTORIES; MARINE-ALGAE; BROWN-ALGAE; PHAEOPHYTA; RHODOPHYTA	Forty Ectocarpus siliculosus isolates from a wide geographical range, including gametophyte and sporophyte plants, have all been acclimated to the same salinity for several years. Their salinity tolerances in respect of cell viability, photosynthesis and dark respiration were evaluated over the salinity range: 8 to 96-parts-per-thousand. Significant differences in the physiological tolerances to salt stress compared with viability measurements were evident. Genotypic differences in salt tolerances between groupings of the isolates, and also differences in responses of gametophyte and sporophyte generations were found. However, diploid and haploid sporophyte material had similar tolerances. Triploid and tetraploid sporophytes did not have improved tolerances over those of diploid plants. Culture plants originating from low salinities in the Baltic Sea had broader tolerances than field material collected from Baltic waters of similar salinity.		THOMAS, DN (corresponding author), UNIV BREMEN, DEPT MARINE BOT, FACHBEREICH 2, W-2800 BREMEN 33, GERMANY.		Thomas, David N./B-1448-2010	Thomas, David N./0000-0001-8832-5907			ALLENDER BM, 1977, J EXP MAR BIOL ECOL, V26, P225, DOI 10.1016/0022-0981(77)90083-1; BOLTON JJ, 1982, MAR BIOL, V66, P89, DOI 10.1007/BF00397259; BOLTON JJ, 1979, ESTUAR COAST MAR SCI, V9, P273, DOI 10.1016/0302-3524(79)90040-9; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; CLAYTON MN, 1988, BOT MAR, V31, P379, DOI 10.1515/botm.1988.31.5.379; CLAYTON MN, 1982, BOT MAR, V25, P111, DOI 10.1515/botm.1982.25.3.111; De Wreede RE, 1988, PLANT REPROD ECOLOGY, P267; Dixon PS, 1965, BOT GOTHOBURG, V3, P67; HENRY EC, 1988, BOT MAR, V31, P353, DOI 10.1515/botm.1988.31.4.353; JEFFREY SW, 1975, BIOCHEM PHYSIOL PFL, V167, P191, DOI 10.1016/s0015-3796(17)30778-3; KIRST GO, 1990, ANNU REV PLANT PHYS, V41, P21, DOI 10.1146/annurev.pp.41.060190.000321; KULLENBERG G., 1981, BALTIC SEA, V30, P135, DOI DOI 10.1016/S0422-9894(08)70140-5; Lobban CS, 1985, PHYSL ECOLOGY SEAWEE, P242; LUNING K, 1988, J PHYCOL, V24, P310, DOI 10.1111/j.1529-8817.1988.tb00178.x; MAGGS CA, 1988, BOT MAR, V31, P465, DOI 10.1515/botm.1988.31.6.465; MATHIESON AC, 1975, MAR BIOL, V33, P207, DOI 10.1007/BF00390924; MATHIESON AC, 1989, BOT MAR, V32, P419, DOI 10.1515/botm.1989.32.5.419; MATHIESON AC, 1975, J EXP MAR BIOL ECOL, V20, P237, DOI 10.1016/0022-0981(75)90085-4; MCLACHLAN J, 1984, HELGOLANDER MEERESUN, V38, P319, DOI 10.1007/BF02027684; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1977, BRIT PHYCOL J, V12, P131; MULLER DG, 1972, SOC BOT FR MEM, P87; Muller DG, 1975, LYNGB ARCH PROTISTEN, V117, P297; NELSON WA, 1989, BOT MAR, V32, P41, DOI 10.1515/botm.1989.32.1.41; NICHOLS WH, 1979, POLYPLOIDY BIOL RELE, P151; NOVACZEK I, 1987, CAN J BOT, V65, P57, DOI 10.1139/b87-007; NOVACZEK I, 1986, CAN J BOT, V64, P2414, DOI 10.1139/b86-321; REED RH, 1980, J EXP BOT, V31, P1521, DOI 10.1093/jxb/31.6.1521; REED RH, 1984, J EXP MAR BIOL ECOL, V76, P131, DOI 10.1016/0022-0981(84)90061-3; REED RH, 1979, ESTUAR COAST MAR SCI, V8, P251, DOI 10.1016/0302-3524(79)90095-1; REED RH, 1983, J EXP MAR BIOL ECOL, V68, P169, DOI 10.1016/0022-0981(83)90158-2; REED RH, 1983, BOT MAR, V26, P409, DOI 10.1515/botm.1983.26.9.409; RUSSELL G, 1985, BRIT PHYCOL J, V20, P87, DOI 10.1080/00071618500650111; RUSSELL G, 1988, HELGOLANDER MEERESUN, V42, P243, DOI 10.1007/BF02366044; RUSSELL G, 1975, ESTUAR COAST MAR SCI, V3, P91, DOI 10.1016/0302-3524(75)90008-0; RUSSELL G, 1986, OCEANOGR MAR BIOL, V24, P309; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; RUSSELL G, 1985, J MAR BIOL ASSOC UK, V65, P343, DOI 10.1017/S0025315400050463; RUSSELL G, 1987, SPECIAL PUBLICATION, V5, P35; RUSSELL G, 1973, P INT C MAR CORROSIO, V3, P719; SAGA N, 1989, Japanese Journal of Phycology, V37, P129; SAGA N, 1989, B HOKKAIDO REG FISH, V53, P43; STEIN J R, 1973, P448; TAL M, 1979, POLYPLOIDY BIOL RELE, P61; THOMAS DN, 1990, ESTUAR COAST SHELF S, V30, P201, DOI 10.1016/0272-7714(90)90064-X; Truesdale G.A., 1955, J APPL CHEM-USSR, V5, P53, DOI 10.1002/jctb. 5010050201; VANDENHOEK C, 1982, BIOL J LINN SOC, V18, P81; WANG JC, 1989, BRIT PHYCOL J, V24, P367, DOI 10.1080/00071618900650381; WHITTICK A, 1977, J EXP MAR BIOL ECOL, V29, P223, DOI 10.1016/0022-0981(77)90067-3; YARISH C, 1979, MAR BIOL, V51, P289, DOI 10.1007/BF00386809; YARISH C, 1986, BOT MAR, V29, P215, DOI 10.1515/botm.1986.29.3.215; YARISH C, 1984, HELGOLANDER MEERESUN, V38, P273, DOI 10.1007/BF01997485; YOUNG AJ, 1987, J EXP BOT, V38, P1309, DOI 10.1093/jxb/38.8.1309	54	26	27	0	14	GEORG THIEME VERLAG KG	STUTTGART	RUDIGERSTR 14, D-70469 STUTTGART, GERMANY	0932-8629			BOT ACTA	Bot. Acta	FEB	1991	104	1					26	36		10.1111/j.1438-8677.1991.tb00190.x			11	Plant Sciences	Plant Sciences	FE723	WOS:A1991FE72300004					2021-04-07	
J	WINHAUER, T; JAGER, S; VALENTIN, K; ZETSCHE, K				WINHAUER, T; JAGER, S; VALENTIN, K; ZETSCHE, K			STRUCTURAL SIMILARITIES BETWEEN PSBA GENES FROM RED AND BROWN-ALGAE	CURRENT GENETICS			English	Article						RHODOPHYTA; CHROMOPHYTA; PSBA GENE; PLASTID EVOLUTION	THYLAKOID MEMBRANE-PROTEIN; PHOTOSYSTEM-II; NUCLEOTIDE-SEQUENCE; CHLOROPLAST GENE; RUBISCO OPERON; CYANOBACTERIUM; PLASTIDS; FAMILY; ORGANIZATION; POLYPEPTIDE	The single copy psbA genes from the multicellular red alga Antithamnion spec. and the brown alga Ectocarpus siliculosus have been cloned and sequenced and monocistronic transcripts have been detected. Both genes contain an insertion of 21 bp at the 3' end which was also found in cyanobacteria and which is absent in chloroplasts and the chlorophyll b-containing prochlorophyte Prochlorothrix hollandica. These findings are in agreement with the hypothesis of a polyphyletic origin of plastids. Plastids of red and brown algae appear to be closely related.	UNIV GIESSEN,INST PFLANZENPHYSIOL,HEINRICH BUFF RING,W-6300 GIESSEN,GERMANY			Valentin, Klaus/G-5862-2014	Valentin, Klaus/0000-0001-7401-9423			ALDRICH J, 1986, NUCLEIC ACIDS RES, V14, P9536, DOI 10.1093/nar/14.23.9536; BARBER J, 1987, FEBS LETT, V220, P67, DOI 10.1016/0014-5793(87)80877-3; CAVALIERSMITH T, 1987, ANN NY ACAD SCI, V503, P17, DOI 10.1111/j.1749-6632.1987.tb40596.x; CURTIS SE, 1984, PLANT MOL BIOL, V3, P249, DOI 10.1007/BF00029661; ERICKSON JM, 1984, EMBO J, V3, P2753, DOI 10.1002/j.1460-2075.1984.tb02206.x; GIBBS SP, 1981, ANN NY ACAD SCI, V361, P193; GIOVANNONI SJ, 1988, J BACTERIOL, V170, P3584, DOI 10.1128/jb.170.8.3584-3592.1988; GOLDEN SS, 1986, EMBO J, V5, P2789, DOI 10.1002/j.1460-2075.1986.tb04569.x; HIGGINS DG, 1988, GENE, V73, P237, DOI 10.1016/0378-1119(88)90330-7; JANSSEN I, 1989, CURR GENET, V15, P335, DOI 10.1007/BF00419913; KOSTRZEWA M, 1990, CURR GENET, V18, P465, DOI 10.1007/BF00309918; KOWALLIK KV, 1989, CHROMOPHYTE ALGAE PR, P99; KYLE DJ, 1985, PHOTOCHEM PHOTOBIOL, V41, P107, DOI 10.1111/j.1751-1097.1985.tb03456.x; LINK G, 1984, NUCLEIC ACIDS RES, V12, P945, DOI 10.1093/nar/12.2.945; MAID U, 1990, CURR GENET, V17, P255, DOI 10.1007/BF00312617; MAID U, 1991, PLANT MOL BIOL, V16, P537, DOI 10.1007/BF00023420; Margulis L., 1981, SYMBIOSIS CELL EVOLU; MEYER TE, 1986, P NATL ACAD SCI USA, V83, P217, DOI 10.1073/pnas.83.2.217; MORDEN CW, 1989, NATURE, V337, P382, DOI 10.1038/337382a0; MULLIGAN B, 1984, P NATL ACAD SCI-BIOL, V81, P2693, DOI 10.1073/pnas.81.9.2693; OHYAMA K, 1986, NATURE, V322, P572, DOI 10.1038/322572a0; OSIEWACZ HD, 1987, NUCLEIC ACIDS RES, V15, P10585, DOI 10.1093/nar/15.24.10585; PALMER JD, 1985, ANNU REV GENET, V19, P325, DOI 10.1146/annurev.ge.19.120185.001545; RUF M, 1988, FEBS LETT, V240, P41, DOI 10.1016/0014-5793(88)80336-3; SHINE J, 1975, NATURE, V254, P34, DOI 10.1038/254034a0; VALENTIN K, 1990, MOL GEN GENET, V222, P425, DOI 10.1007/BF00633849; VALENTIN K, 1989, CURR GENET, V16, P203, DOI 10.1007/BF00391478; VALENTIN K, 1990, PLANT MOL BIOL, V15, P575, DOI 10.1007/BF00017832; VALENTIN K, 1990, CURR GENET, V18, P199, DOI 10.1007/BF00318380; VANDENEYNDE H, 1988, J MOL EVOL, V27, P126, DOI 10.1007/BF02138372; WALKER JM, 1984, METHODS MOL BIOL, V2; WHATLEY JM, 1989, CHROMOPHYTE ALGAE PR, P125; ZURAWSKI G, 1982, P NATL ACAD SCI-BIOL, V79, P7699, DOI 10.1073/pnas.79.24.7699	33	24	26	0	1	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0172-8083			CURR GENET	Curr. Genet.		1991	20	1-2					177	180		10.1007/BF00312783			4	Genetics & Heredity	Genetics & Heredity	FY553	WOS:A1991FY55300027	1934114				2021-04-07	
J	KAWAI, H; MULLER, DG; FOLSTER, E; HADER, DP				KAWAI, H; MULLER, DG; FOLSTER, E; HADER, DP			PHOTOTACTIC RESPONSES IN THE GAMETES OF THE BROWN ALGA, ECTOCARPUS-SILICULOSUS	PLANTA			English	Article									FRIEDRICH ALEXANDER UNIV ERLANGEN,DEPT BOT,STAUDTSTR 5,W-8520 ERLANGEN,GERMANY; HOKKAIDO UNIV,FAC SCI,DEPT BOT,SAPPORO,HOKKAIDO 060,JAPAN; UNIV CONSTANCE,FAC BIOL,W-7750 CONSTANCE,GERMANY							BUDER J, 1919, JB WISS BOT, V58, P105; COLEMAN AW, 1988, J PHYCOL, V24, P118; Diehn B, 1973, BEHAVIOUR MICROORGAN, P83; DODGE JD, 1975, FINE STRUCTURE ALGAL; FOSTER KW, 1980, MICROBIOL REV, V44, P572, DOI 10.1128/MMBR.44.4.572-630.1980; GELLER A, 1981, J EXP BIOL, V92, P53; HADER DP, 1986, CURR MICROBIOL, V14, P157, DOI 10.1007/BF01568368; HADER DP, 1985, PHOTOCHEM PHOTOBIOL, V42, P509, DOI 10.1111/j.1751-1097.1985.tb01602.x; HADER DP, 1987, ARCH MICROBIOL, V147, P179, DOI 10.1007/BF00415281; KAWAI H, 1989, PHYCOLOGIA, V28, P222, DOI 10.2216/i0031-8884-28-2-222.1; KAWAI H, 1988, J PHYCOL, V24, P114; KAWAI H, 1990, IN PRESS PROTOPLASMA; MOESTRUP O, 1982, PHYCOLOGIA, V21, P427, DOI 10.2216/i0031-8884-21-4-427.1; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1987, PHOTOCHEM PHOTOBIOL, V46, P1003, DOI 10.1111/j.1751-1097.1987.tb04884.x; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; STARR RC, 1978, J PHYCOL, V14, P47, DOI 10.1111/j.1529-8817.1978.tb02507.x; WALNE PL, 1967, PLANTA, V77, P325, DOI 10.1007/BF00389319	18	42	43	0	5	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0032-0935			PLANTA	Planta	SEP	1990	182	2					292	297					6	Plant Sciences	Plant Sciences	DX287	WOS:A1990DX28700020	24197109				2021-04-07	
J	MULLER, DG; KAWAI, H; STACHE, B; LANKA, S				MULLER, DG; KAWAI, H; STACHE, B; LANKA, S			A VIRUS-INFECTION IN THE MARINE BROWN ALGA ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	BOTANICA ACTA			English	Article										MULLER, DG (corresponding author), UNIV KONSTANZ,FAK BIOL,POSTFACH 5560,W-7750 CONSTANCE 1,GERMANY.						Cardinal A., 1964, NOVA HEDWIGIA, V15, P1; CLITHEROE SB, 1974, J ULTRA MOL STRUCT R, V49, P211, DOI 10.1016/S0022-5320(74)80032-8; DODDS AJ, 1983, CRITICAL APPRAISAL V, P177; KUROIWA T, 1984, EXP CELL RES, V153, P259, DOI 10.1016/0014-4827(84)90469-5; LACLAIRE JW, 1977, PROTOPLASMA, V93, P127, DOI 10.1007/BF01276287; LEMKE PA, 1976, ANNU REV MICROBIOL, V30, P105, DOI 10.1146/annurev.mi.30.100176.000541; Maniatis T., 1982, MOL CLONING; MARKEY D R, 1974, Protoplasma, V80, P223, DOI 10.1007/BF01666361; MEINTS RH, 1988, VIROLOGY, V164, P15, DOI 10.1016/0042-6822(88)90614-9; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1988, HELGOLANDER MEERESUN, V42, P469, DOI 10.1007/BF02365621; OLIVEIRA L, 1978, ANN BOT-LONDON, V42, P439, DOI 10.1093/oxfordjournals.aob.a085477; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; Plattner H, 1987, ELEKTRONENMIKROSKOPI; REISSER W, 1989, BOT ACTA, V102, P117, DOI 10.1111/j.1438-8677.1989.tb00076.x; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1; STARR RC, 1978, J PHYCOL, V14, P47, DOI 10.1111/j.1529-8817.1978.tb02507.x; TOTH R, 1972, J PHYCOL, V8, P126, DOI 10.1111/j.1529-8817.1972.tb04011.x; USHIYAMA R, 1985, MICROBIOL SCI, V2, P181; VANETTEN JL, 1987, PLANT MICROBE INTERA, V2	21	65	65	0	9	GEORG THIEME VERLAG	STUTTGART	P O BOX 30 11 20, D-70451 STUTTGART, GERMANY	0932-8629			BOT ACTA	Bot. Acta	FEB	1990	103	1					72	82					11	Plant Sciences	Plant Sciences	CZ647	WOS:A1990CZ64700014					2021-04-07	
J	BOLAND, W; HOEVER, FP; KRUGER, BW				BOLAND, W; HOEVER, FP; KRUGER, BW			APPLICATION OF MOLECULAR MODELING TECHNIQUES TO PHEROMONES OF THE MARINE BROWN-ALGAE CUTLERIA-MULTIFIDA AND ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE) - METALLOPROTEINS AS CHEMORECEPTORS	ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES			English	Article									BAYER AG,D-5090 LEVERKUSEN,FED REP GER	BOLAND, W (corresponding author), INST ORGAN CHEM,RICHARD WILLSTATTER ALLEE,D-7500 KARLSRUHE,FED REP GER.		Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			ALLEN FH, 1983, ACCOUNTS CHEM RES, V16, P146, DOI 10.1021/ar00089a001; ALLINGER NL, QUANTUM CHEM PROGRAM, V11; BESTMANN HJ, 1984, 113 VERH GES DTSCH N; BOLAND W, 1979, J ORG CHEM, V44, P4819, DOI 10.1021/jo00394a016; BOLAND W, 1982, EUR J BIOCHEM, V126, P173, DOI 10.1111/j.1432-1033.1982.tb06763.x; BOLAND W, 1981, Z NATURFORSCH C, V36, P262; BOLAND W, 1984, EUR J BIOCHEM, V144, P169, DOI 10.1111/j.1432-1033.1984.tb08445.x; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLAND W, 1984, HELV CHIM ACTA, V67, P616, DOI 10.1002/hlca.19840670230; BURKETT U, 1982, ACS MONOGRAPH SERIES, V177; CHAPMAN OL, 1978, SCIENCE, V201, P926, DOI 10.1126/science.201.4359.926; GOLDSCHMIDT Z, 1985, J ORGANOMET CHEM, V282, P369, DOI 10.1016/0022-328X(85)87196-5; HUTTNER G, 1972, CHEM BER-RECL, V105, P3936, DOI 10.1002/cber.19721051221; KAFKA WA, 1975, Z NATURFORSCH C, VC 30, P278; LILJEFORS T, 1987, J CHEM ECOL, V13, P2023, DOI 10.1007/BF01041729; MARSAGLIA GA, 1985, COMPUTER SCI STAT IN, P1; MARSHALL GR, 1984, DRUG DESIGN FACT FAN, P35; MULLER DG, 1976, Z PFLANZENPHYSIOL, V80, P121; RETTIG MF, 1981, J AM CHEM SOC, V103, P2980, DOI 10.1021/ja00401a012; TERLINDEN R, 1983, HELV CHIM ACTA, V66, P466, DOI 10.1002/hlca.19830660207; TERLINDEN R, UNPUB	21	5	5	0	2	VERLAG Z NATURFORSCH	TUBINGEN	POSTFACH 2645, W-7400 TUBINGEN, GERMANY	0939-5075			Z NATURFORSCH C	Z.Naturforsch.(C)	SEP-OCT	1989	44	9-10					829	837					9	Biochemistry & Molecular Biology; Pharmacology & Pharmacy	Biochemistry & Molecular Biology; Pharmacology & Pharmacy	AX199	WOS:A1989AX19900023					2021-04-07	
J	MULLER, DG; SCHMID, CE				MULLER, DG; SCHMID, CE			QUALITATIVE AND QUANTITATIVE-DETERMINATION OF PHEROMONE SECRETION IN FEMALE GAMETES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE)	BIOLOGICAL CHEMISTRY HOPPE-SEYLER			English	Article										MULLER, DG (corresponding author), UNIV CONSTANCE,FAK BIOL,POB 5560,D-7750 CONSTANCE,FED REP GER.						ALLEN NS, 1984, CELL MOTIL CYTOSKEL, V4, P25, DOI 10.1002/cm.970040104; BARBIER M, 1982, PHEROMONES ASPECTS B; BOLAND W, 1984, EUR J BIOCHEM, V144, P169, DOI 10.1111/j.1432-1033.1984.tb08445.x; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLAND W, 1985, EUR J BIOCHEM, V147, P83, DOI 10.1111/j.1432-1033.1985.tb08722.x; Boland W, 1984, ANAL VOLATILES, P371; CLAYTON MN, 1984, J PHYCOL, V20, P276, DOI 10.1111/j.0022-3646.1984.00276.x; GELLER A, 1981, J EXP BIOL, V92, P53; GROB K, 1976, J CHROMATOGR, V117, P285, DOI 10.1016/0021-9673(76)80005-2; JAENICKE L, 1973, Justus Liebigs Annalen der Chemie, P1252; KARLSON P, 1959, NATURWISSENSCHAFTEN, V46, P63, DOI 10.1007/BF00599084; LUNING K, 1985, MEERESBOTANIK; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MAIER I, 1987, Z NATURFORSCH C, V42, P948; MAIER I, 1988, IN PRESS NATURWISSEN, V75; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1968, PLANTA, V81, P160, DOI 10.1007/BF00417445; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1976, ARCH MICROBIOL, V109, P89, DOI 10.1007/BF00425117; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1985, Z NATURFORSCH C, V40, P457; MULLER DG, 1982, NATURWISSENSCHAFTEN, V69, P290, DOI 10.1007/BF00396442; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; MULLER DG, 1980, NATURWISSENSCHAFTEN, V67, P462, DOI 10.1007/BF00405647; PENZLIN H, 1980, LEHRBUCH TIERPHYSIOL; STARR RC, 1978, J PHYCOL, V14, P47, DOI 10.1111/j.1529-8817.1978.tb02507.x	27	25	25	0	7	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0177-3593			BIOL CHEM H-S	Biol. Chem. Hoppe-Seyler	AUG	1988	369	8					647	653		10.1515/bchm3.1988.369.2.647			7	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	P8263	WOS:A1988P826300003	3214549				2021-04-07	
J	MULLER, DG				MULLER, DG			STUDIES ON SEXUAL COMPATIBILITY BETWEEN ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE) FROM CHILE AND THE MEDITERRANEAN-SEA	HELGOLANDER MEERESUNTERSUCHUNGEN			English	Article										MULLER, DG (corresponding author), UNIV CONSTANCE,FAK BIOL,D-7750 CONSTANCE,FED REP GER.						Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; BOLTON JJ, 1983, MAR BIOL, V73, P131, DOI 10.1007/BF00406880; Dobzhansky Theodosius, 1977, EVOLUTION; LUNING K, 1985, MEERESBOTANIK; MAIER I, 1986, BIOL BULL, V170, P145, DOI 10.2307/1541801; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1976, ARCH MICROBIOL, V109, P89, DOI 10.1007/BF00425117; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1980, NATURWISSENSCHAFTEN, V67, P462, DOI 10.1007/BF00405647; Newton L, 1931, HDB BRIT SEAWEEDS; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; STARR RC, 1978, J PHYCOL, V14, P47, DOI 10.1111/j.1529-8817.1978.tb02507.x	12	12	12	0	3	BIOLOGISCHE ANSTALT HELGOLAND	HAMBURG	NOTKESTRASSE 31, 22607 HAMBURG, GERMANY	0174-3597			HELGOLANDER MEERESUN	Helgol. Meeresunters.		1988	42	3-4					469	476		10.1007/BF02365621			8	Marine & Freshwater Biology; Oceanography	Marine & Freshwater Biology; Oceanography	U6943	WOS:A1988U694300006		Bronze			2021-04-07	
J	HALL, A; BAKER, AJM				HALL, A; BAKER, AJM			SETTLEMENT AND GROWTH OF COPPER-TOLERANT ECTOCARPUS-SILICULOSUS (DILLW) LYNGBYE ON DIFFERENT COPPER-BASED ANTIFOULING SURFACES UNDER LABORATORY CONDITIONS .2. A COMPARISON OF THE EARLY STAGES OF FOULING USING LIGHT AND SCANNING ELECTRON-MICROSCOPY	JOURNAL OF MATERIALS SCIENCE			English	Article										HALL, A (corresponding author), UNIV SHEFFIELD,DEPT BOT,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND.						BULOW CL, 1945, T ELECTROCHEM SOC, V87, P127, DOI 10.1149/1.3071636; EFIRD KD, 1975, MATER PERFORMANCE, V14, P37; HALL A, 1985, J MATER SCI, V20, P1111, DOI 10.1007/BF00585756; HALL A, 1982, 306 INT COPP RES ASS; LAQUE FL, 1945, T ELECTROCHEM SOC, V87, P103, DOI 10.1149/1.3071635; LAQUE FL, 1974, 3RD P INT C MAR CORR, P2; LAQUE FL, 1948, CORROSION HDB, P383; RUSSELL G, 1971, SEA BREEZES      JUL, P512; SWAIN GWJ, 1982, J MATER SCI, V17, P1079, DOI 10.1007/BF00543527	9	3	3	0	5	CHAPMAN HALL LTD	LONDON	2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN	0022-2461			J MATER SCI	J. Mater. Sci.	APR	1986	21	4					1240	1252		10.1007/BF00553258			13	Materials Science, Multidisciplinary	Materials Science	A5188	WOS:A1986A518800021					2021-04-07	
J	HALL, A; BAKER, AJM				HALL, A; BAKER, AJM			SETTLEMENT AND GROWTH OF COPPER-TOLERANT ECTOCARPUS-SILICULOSUS (DILLW) LYNGBYE ON DIFFERENT COPPER-BASED ANTIFOULING SURFACES UNDER LABORATORY CONDITIONS .1. CORROSION TRIALS IN SEAWATER AND DEVELOPMENT OF AN ALGAL CULTURE SYSTEM	JOURNAL OF MATERIALS SCIENCE			English	Article										HALL, A (corresponding author), UNIV SHEFFIELD,DEPT BOT,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND.						EFIRD KD, 1975, MATER PERFORMANCE, V14, P37; GILBERT PT, 1981, APR CORR 81 C TOR, P16; HALL A, 1981, BOT MAR, V24, P223, DOI 10.1515/botm.1981.24.4.223; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; HALL A, 1982, 306 INT COPP RES ASS; LAQUE FL, 1948, CORROSION HDB, P383; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; RUSSELL G, 1973, 3RD P INT C MAR CORR, P719; TODD B, 1974, EC CORROSION CONTROL, P46	9	6	6	0	4	CHAPMAN HALL LTD	LONDON	2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN	0022-2461			J MATER SCI	J. Mater. Sci.		1985	20	3					1111	1118		10.1007/BF00585756			8	Materials Science, Multidisciplinary	Materials Science	AER80	WOS:A1985AER8000043					2021-04-07	
J	BOLAND, W; JAENICKE, L; MULLER, DG; PETERS, A				BOLAND, W; JAENICKE, L; MULLER, DG; PETERS, A			DIFFERENTIATION OF ALGAL CHEMORECEPTORS .6. A COMPARATIVE RECEPTOR STUDY WITH 2 SEAWEED PAIRS - CUTLERIA-MULTIFIDA SYRINGODERMA-PHINNEYI AND DESMARESTIA-ACULEATA ECTOCARPUS-SILICULOSUS (PHACOPHYCEAE)	EUROPEAN JOURNAL OF BIOCHEMISTRY			English	Article									UNIV CONSTANCE,FACHBEREICH BIOL,D-7750 CONSTANCE,FED REP GER	BOLAND, W (corresponding author), UNIV COLOGNE,INST BIOCHEM,D-5000 COLOGNE 41,FED REP GER.		Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			AKINTOBI T, 1975, THESIS U COLOGNE; BERG HC, 1977, BIOPHYS J, V20, P193, DOI 10.1016/S0006-3495(77)85544-6; BOHLMANN F, 1979, PHYTOCHEMISTRY, V18, P79, DOI 10.1016/S0031-9422(00)90920-8; BOLAND W, 1982, Z NATURFORSCH C, V37, P5; BOLAND W, 1982, HELV CHIM ACTA, V65, P2355, DOI 10.1002/hlca.19820650742; BOLAND W, 1982, EUR J BIOCHEM, V126, P173, DOI 10.1111/j.1432-1033.1982.tb06763.x; BOLAND W, 1981, Z NATURFORSCH C, V36, P262; BOLAND W, 1983, EUR J BIOCHEM, V134, P97, DOI 10.1111/j.1432-1033.1983.tb07536.x; BOLAND W, 1984, HELV CHIM ACTA, V67, P616, DOI 10.1002/hlca.19840670230; BOLAND W, 1983, HELV CHIM ACTA, V66, P1905, DOI 10.1002/hlca.19830660632; BOLAND W, 1981, LIEBIGS ANN CHEM, P2266; DANSLAX, 1967, TASCHENBUCH CHEM PHY, V1; GELLER A, 1981, J EXP BIOL, V92, P53; GORISCH H, 1984, APPL BIOCH; HENRY EC, 1983, PHYCOLOGIA, V22, P387, DOI 10.2216/i0031-8884-22-4-387.1; JACOB F, 1977, SCIENCE, V196, P1161, DOI 10.1126/science.860134; JAENICKE L, 1974, J AM CHEM SOC, V96, P3324, DOI 10.1021/ja00817a056; JAENICKE L, 1982, ANGEW CHEM INT EDIT, V21, P643, DOI 10.1002/anie.198206433; Jaenicke L, 1982, ANGEW CHEM, V94, P659; KOSHLAND DE, 1979, PHYSIOL REV, V59, P811; MAIER I, 1984, NATURWISSENSCHAFTEN, V71, P48, DOI 10.1007/BF00365988; MOORE RE, 1973, J ORG CHEM, V39, P2201; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1981, BRIT PHYCOL J, V16, P351; MULLER DG, 1982, NATURWISSENSCHAFTEN, V69, P501, DOI 10.1007/BF00365822; MULLER DG, 1982, NATURWISSENSCHAFTEN, V69, P290, DOI 10.1007/BF00396442; MULLER DG, 1979, PURE APPL CHEM, V51, P1885, DOI 10.1351/pac197951091885; MULLER DG, 1971, SCIENCE WASH, V161, P815; MULLER DG, 1976, Z PFLANZENPHYSIOL, V80, P121; OHLOFF G, 1969, HELV CHIM ACTA, V52, P880, DOI 10.1002/hlca.19690520403; SCHOTTEN T, 1982, THESIS U COLOGNE; von Smoluchowski M, 1917, Z PHYS CHEM-STOCH VE, V92, P129; WYNNE M J, 1976, Phycologia, V15, P435, DOI 10.2216/i0031-8884-15-3-435.1	34	24	25	0	2	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0014-2956			EUR J BIOCHEM	Eur. J. Biochem.		1984	144	1					169	176		10.1111/j.1432-1033.1984.tb08445.x			8	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	TN895	WOS:A1984TN89500022	6541129				2021-04-07	
J	BOLAND, W; MARNER, FJ; JAENICKE, L; MULLER, DG; FOLSTER, E				BOLAND, W; MARNER, FJ; JAENICKE, L; MULLER, DG; FOLSTER, E			COMPARATIVE RECEPTOR STUDY IN GAMETE CHEMOTAXIS OF THE SEAWEEDS ECTOCARPUS-SILICULOSUS AND CUTLERIA-MULTIFIDA - AN APPROACH TO INTERSPECIFIC COMMUNICATION OF ALGAL GAMETES	EUROPEAN JOURNAL OF BIOCHEMISTRY			English	Article									UNIV KONSTANZ,FACHBEREICH BIOL,D-7750 KONSTANZ 1,FED REP GER	BOLAND, W (corresponding author), UNIV KOLN,INST BIOCHEM,D-5000 KOLN 1,FED REP GER.		Boland, Wilhelm/K-7762-2012	Boland, Wilhelm/0000-0001-6784-2534			BOLAND W, 1982, HELV CHIM ACTA, V65, P2355, DOI 10.1002/hlca.19820650742; BOLAND W, 1982, EUR J BIOCHEM, V126, P173, DOI 10.1111/j.1432-1033.1982.tb06763.x; BOLAND W, 1981, Z NATURFORSCH C, V36, P262; BOLAND W, 1981, LIEBIGS ANN CHEM, P2266; BOLAND W, HELV CHIM ACTA; DERENBACH JB, 1980, MAR ECOL PROG SER, V3, P357, DOI 10.3354/meps003357; GELLER A, 1981, J EXP BIOL, V92, P53; JAENICKE L, 1974, J AM CHEM SOC, V96, P3324, DOI 10.1021/ja00817a056; JAENICKE L, 1973, LIEBIGS ANN CHEM, P1252; Jaenicke L, 1982, ANGEW CHEM, V94, P659; JAENICKE L, 1982, ANGEW CHEM INT EDIT, V94, P643; JAENICKE L, 1979, MECHANISM BIOL RECOG, P395; MARNER FJ, 1975, CHEM BER-RECL, V108, P2202, DOI 10.1002/cber.19751080706; MOORE RE, 1977, ACCOUNTS CHEM RES, V10, P40, DOI 10.1021/ar50110a002; MOORE RE, 1978, MARINE NATURAL PRODU, P43; MULLER DG, 1968, PLANTA, V81, P160, DOI 10.1007/BF00417445; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1970, NATURWISSENSCHAFTEN, V57, P357; MULLER DG, 1979, NATURE, V279, P430, DOI 10.1038/279430a0; MULLER DG, 1974, BIOCHEM PHYSIOL PFL, V165, P212; MULLER DG, 1982, NATURWISSENSCHAFTEN, V69, P290, DOI 10.1007/BF00396442; MULLER DG, 1981, NATURWISSENSCHAFTEN, V68, P478, DOI 10.1007/BF01047524; MULLER DG, 1979, PURE APPL CHEM, V51, P1885, DOI 10.1351/pac197951091885; MULLER DG, 1981, 10TH P INT SEAW S, P57; MULLER DG, 1971, SCIENCE WASH, V161, P815; MULLER DG, 1976, Z PFLANZENPHYSIOL, V80, P121	26	34	35	0	4	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0014-2956			EUR J BIOCHEM	Eur. J. Biochem.		1983	134	1					97	103		10.1111/j.1432-1033.1983.tb07536.x			7	Biochemistry & Molecular Biology	Biochemistry & Molecular Biology	RA633	WOS:A1983RA63300015	6683168	Bronze			2021-04-07	
J	BOLTON, JJ				BOLTON, JJ			ECOCLINAL VARIATION IN ECTOCARPUS-SILICULOSUS (PHAEOPHYCEAE) WITH RESPECT TO TEMPERATURE GROWTH OPTIMA AND SURVIVAL LIMITS	MARINE BIOLOGY			English	Article									BIOL ANSTALT HELGOLAND,D-2192 HELGOLAND,FED REP GER				Bolton, John/0000-0003-0589-9564			BIEBL R, 1970, PROTOPLASMA, V69, P61, DOI 10.1007/BF01276652; BIEBL R, 1958, PROTOPLASMA, V50, P217; BIEBL R, 1939, JB WISS BOT, V88, P389; Biebl R., 1968, FLORA              B, V157, P327; BIEBL RICHARD, 1962, BOT MARINA, V4, P241, DOI 10.1515/botm.1962.4.3-4.241; BOLTON JJ, 1982, MAR BIOL, V66, P89, DOI 10.1007/BF00397259; CHEN LCM, 1980, BOT MAR, V23, P441; EDWARDS P, 1973, CONTRIB MAR SCI, V17, P15; HOEK C. VAN DEN, 1975, PHYCOLOGIA, V14, P317, DOI DOI 10.2216/I0031-8884-14-4-317.1; KAIN JM, 1971, J MAR BIOL ASSOC UK, V51, P387, DOI 10.1017/S0025315400031866; KING RJ, 1970, AUST J MAR FRESH RES, V21, P47; LEE RKS, 1980, PUBLICATIONS BOTANY, V9; LUNING K, 1978, PHYCOLOGIA, V17, P293, DOI 10.2216/i0031-8884-17-3-293.1; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1979, PHYCOLOGIA, V18, P312, DOI 10.2216/i0031-8884-18-4-312.1; MULLER DG, 1972, SOC BOT FR MEMOIRES, V8798; RUSSELL G, 1975, ESTUAR COAST MAR SCI, V3, P91, DOI 10.1016/0302-3524(75)90008-0; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; SEARS JA, 1971, THESIS U MASSACHUSET; Stephenson T.A., 1972, LIFE TIDEMARKS ROCKY; Taylor WR, 1957, MARINE ALGAE NE COAS; TERAMURA AH, 1979, CAN J BOT, V57, P2559, DOI 10.1139/b79-304; van den Hoek C, 1967, BLUMEA, V15, P63; Watanase A., 1968, CULTURES COLLECTIONS, P47	24	73	73	0	7	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0025-3162			MAR BIOL	Mar. Biol.		1983	73	2					131	138		10.1007/BF00406880			8	Marine & Freshwater Biology	Marine & Freshwater Biology	QK805	WOS:A1983QK80500003					2021-04-07	
J	GEISSLER, U				GEISSLER, U			A POLLUTED SECTION OF THE RIVER WERRA, AN INLAND HABITAT OF ECTOCARPUS-CONFERVOIDES (ROTH) KJELLMANN	NOVA HEDWIGIA			German	Article										GEISSLER, U (corresponding author), FREE UNIV BERLIN,INST SYSTEMAT BOT & PFLANZENGEOG,D-1000 BERLIN 33,FED REP GER.							0	14	14	0	1	GEBRUDER BORNTRAEGER	STUTTGART	JOHANNESSTR 3A, D-70176 STUTTGART, GERMANY	0029-5035			NOVA HEDWIGIA	Nova Hedwigia		1983	37	1					193	217					25	Plant Sciences	Plant Sciences	RU747	WOS:A1983RU74700006					2021-04-07	
J	HALL, A				HALL, A			COPPER ACCUMULATION IN COPPER-TOLERANT AND NON-TOLERANT POPULATIONS OF MARINE FOULING ALGA, ECTOCARPUS-SILICULOSUS (DILLW) LYNGBYE	BOTANICA MARINA			English	Article									MANCHESTER POLYTECH,DEPT BIOL SCI,CHESTER ST,MANCHESTER ML 5GD,ENGLAND							BRYAN GW, 1969, J MAR BIOL ASSOC UK, V49, P225, DOI 10.1017/S0025315400046531; BRYAN GW, 1973, J MAR BIOL ASSOC UK, V53, P705, DOI 10.1017/S0025315400058902; BRYAN GW, 1976, EFFECTS POLLUTANTS A, V2, P7; FOSTER PL, 1977, NATURE, V269, P322, DOI 10.1038/269322a0; Fuge R, 1974, MARINE POLLUTION B, V5, P9, DOI DOI 10.1016/0025-326X(74)90026-5; GIBSON CE, 1972, J APPL ECOL, V9, P513, DOI 10.2307/2402449; GRYS S, 1976, MIKROCHIM ACTA, V1, P147; GUPTA A B, 1977, Beitraege zur Biologie der Pflanzen, V53, P421; HALL A, 1979, MAR BIOL, V54, P195, DOI 10.1007/BF00395780; MCBRIEN DCH, 1965, PHYSIOL PLANTARUM, V18, P1059, DOI 10.1111/j.1399-3054.1965.tb07005.x; MORRIS O P, 1973, Marine Pollution Bulletin, V4, P159, DOI 10.1016/0025-326X(73)90256-7; MORRIS OP, 1972, THESIS U LIVERPOOL; OVERNELL J, 1975, MAR BIOL, V29, P99; PRESTON A, 1972, Environmental Pollution, V3, P69, DOI 10.1016/0013-9327(72)90018-3; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; SEELIGER U, 1977, MAR POLLUT BULL, V8, P16, DOI 10.1016/0025-326X(77)90398-8; Von Stosch HA, 1963, P INT SEAWEED S, V4, P142; Wainwright S. J., 1975, ECOLOGY RESOURCE DEG, P231; YOUNG E. GORDON, 1958, CANADIAN JOUR BOT, V36, P301	19	25	25	0	2	WALTER DE GRUYTER & CO	BERLIN	GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY	0006-8055			BOT MAR	Bot. Marina		1981	24	4					223	228		10.1515/botm.1981.24.4.223			6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	LP710	WOS:A1981LP71000009					2021-04-07	
J	GELLER, A; MULLER, DG				GELLER, A; MULLER, DG			ANALYSIS OF THE FLAGELLAR BEAT PATTERN OF MALE ECTOCARPUS-SILICULOSUS GAMETES (PHAEOPHYTA) IN RELATION TO CHEMOTACTIC STIMULATION BY FEMALE CELLS	JOURNAL OF EXPERIMENTAL BIOLOGY			English	Article										GELLER, A (corresponding author), UNIV CONSTANCE,FACHBEREICH BIOL,D-7750 CONSTANCE,FED REP GER.						BROKAW CJ, 1965, J EXP BIOL, V43, P155; CHWANG AT, 1971, PROC R SOC SER B-BIO, V178, P327, DOI 10.1098/rspb.1971.0068; GRAY J, 1955, J EXP BIOL, V32, P775; JAHN TL, 1964, J PROTOZOOL, V11, P291, DOI 10.1111/j.1550-7408.1964.tb01756.x; KELLER JB, 1976, BIOPHYS J, V16, P151, DOI 10.1016/S0006-3495(76)85672-X; LOISEAUX S, 1970, T AM MICROSC SOC, V89, P524, DOI 10.2307/3224562; MANTON I, 1953, J EXP BOT, V4, P319, DOI 10.1093/jxb/4.3.319; MANTON I, 1957, J EXP BOT, V8, P294, DOI 10.1093/jxb/8.2.294; MILLER RL, 1975, NATURE, V254, P244, DOI 10.1038/254244a0; MILLER RL, 1979, MAR BIOL, V53, P99, DOI 10.1007/BF00389182; MILLER RL, 1966, J EXP ZOOL, V162, P23, DOI 10.1002/jez.1401620104; MILLER RL, 1970, J EXP ZOOL, V175, P493, DOI 10.1002/jez.1401750409; MILLER RL, 1970, J EXP BIOL, V52, P699; MILLER RL, 1977, J EXP ZOOL, V202, P203, DOI 10.1002/jez.1402020209; MILLER RL, 1976, J CELL BIOL, V70, pA341; MULLER DG, 1976, J PHYCOL, V12, P252, DOI 10.1111/j.0022-3646.1976.00252.x; MULLER DG, 1973, ARCH MIKROBIOL, V91, P313, DOI 10.1007/BF00425051; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1976, ARCH MICROBIOL, V109, P89, DOI 10.1007/BF00425117; MULLER DG, 1979, NATURE, V279, P430, DOI 10.1038/279430a0; MULLER DG, 1977, MARINE NATURAL PRODU, P351; PEARL HW, 1973, LIMNOL OCEANOGR, V18, P802; POMMERVILLE J, 1978, EXP CELL RES, V113, P161, DOI 10.1016/0014-4827(78)90096-4; RIKMENSPOEL R, 1960, PHYS MED BIOL, V5, P167, DOI 10.1088/0031-9155/5/2/306; Sleigh M. A., 1974, CILIA FLAGELLA, P79; VANDENENDE H, 1976, EXPT BOTANY, V9, P97; VANDENHOEK C, 1978, ALGEN, P88; ZIEGLER H, 1962, HDB PFLANZENPHYSIOLO, V17, P485	28	47	47	0	1	COMPANY OF BIOLOGISTS LTD	CAMBRIDGE	BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL	0022-0949			J EXP BIOL	J. Exp. Biol.		1981	92	JUN					53	&					0	Biology	Life Sciences & Biomedicine - Other Topics	LX521	WOS:A1981LX52100005					2021-04-07	
J	PELLEGRINI, L				PELLEGRINI, L			CYTOLOGICAL STUDIES ON PHYSODES IN THE VEGETATIVE CELLS OF CYSTOSEIRA-STRICTA SAUVAGEAU (PHAEOPHYTA, FUCALES)	JOURNAL OF CELL SCIENCE			English	Article							BROWN ALGA ECTOCARPUS; VESICULOSUS L PHAEOPHYTA; ROOT MERISTEMATIC CELLS; FINE-STRUCTURE; ENDOPLASMIC-RETICULUM; PHENOLIC-COMPOUNDS; ULTRASTRUCTURAL-CHANGES; IRIDESCENT BODIES; STORING CELLS; GENUS FUCUS			PELLEGRINI, L (corresponding author), FAC LUMINY, INST CYTOL & BIOL CELLULAIRE, CNRS, LAB 179, 70 ROUTE LEON LACHAMP, F-13288 MARSEILLE 2, FRANCE.						AMELUNXEN F, 1969, Z PFLANZENPHYSIOL, V60, P156; AMELUNXEN F, 1967, Z PFLANZENPHYSIOL, V58, P49; ANDO Y, 1958, B JAP SOC PHYCOL, V6, P28; ANDO Y, 1958, B JAP SOC PHYCOL, V6, P45; ANDO YOSHIAKI, 1951, BOT MAG [TOKYO], V64, P192; BAUR PS, 1974, CAN J BOT, V52, P615, DOI 10.1139/b74-077; BERJAK P, 1972, ANN BOT-LONDON, V36, P73, DOI 10.1093/oxfordjournals.aob.a084578; BISALPUTRA T, 1971, J MICROSC-PARIS, V10, P83; BISALPUTRA T., 1974, ALGAL PHYSL BIOCH, P124; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; BRAWLEY SH, 1977, J CELL SCI, V24, P275; BRAWLEY SH, 1976, J CELL SCI, V20, P255; BUVAT R, 1977, CR ACAD SCI D NAT, V284, P167; BUVAT R, AM J BOT; CARAM B, 1977, J PHYCOL          S2, V13; CARDE JP, 1976, B SOC BOT FR, V123, P181, DOI 10.1080/00378941.1976.10839396; CARDE JP, 1973, J MICROSC-PARIS, V17, P65; Chadefaud, 1932, CR HEBD ACAD SCI, V194, P1675; Chadefaud, 1934, CR HEBD ACAD SCI, V198, P2114; CHADEFAUD M, 1935, THESIS U PARIS; CHAFE SC, 1973, PLANTA, V113, P251, DOI 10.1007/BF00390512; CHARRIER.Y, 1973, J MICROSC-PARIS, V17, P299; CHARRIERELADREI.Y, FEBS LETT AMSTERDAM; CHARRIERELADREIX Y, 1976, PLANTA, V129, P167, DOI 10.1007/BF00390024; CHARRIERELADREIX Y, 1975, J MICROSC-PARIS, V23, pA13; COULOMB C, 1968, CR ACAD SCI D NAT, V267, P843; COULOMB C, 1972, THESIS AIX MARSEILLE; COULOMB C, 1978, THESIS AIX MARSEILLE; CURGY JJ, 1968, J MICROSC-PARIS, V7, P63; DALTON AJ, 1955, ANAT REC, V121, P281; DAVIES JM, 1973, J MAR BIOL ASSOC UK, V53, P237, DOI 10.1017/S0025315400022232; Dodge JD, 1973, FINE STRUCTURE ALGAL; DUMAS C, 1977, CR ACAD SCI D NAT, V284, P1777; DUMAS C, 1974, BOTANISTE, V56, P81; DUMAS C, 1975, THESIS LYON; DUMAS C, 1974, Z BOTANISTE, V56, P59; ENDRESS AG, 1976, PROTOPLASMA, V88, P315, DOI 10.1007/BF01283255; EVANS LV, 1968, NEW PHYTOL, V67, P173, DOI 10.1111/j.1469-8137.1968.tb05467.x; EVANS LV, 1972, NEW PHYTOL, V71, P1173, DOI 10.1111/j.1469-8137.1972.tb01995.x; FELDMANN G, 1972, CR ACAD SCI D NAT, V275, P751; FINERAN BA, 1973, J ULTRA MOL STRUCT R, V43, P75, DOI 10.1016/S0022-5320(73)90071-3; FORBES MA, 1978, BRIT PHYCOL J, V13, P299, DOI 10.1080/00071617800650361; FORREST GI, 1969, BIOCHEM J, V113, P741, DOI 10.1042/bj1130741; FRITSCH FE, 1945, STRUCTURE REPRODUCTI, V2; GAILLARD J, 1962, B SOC PHYCOL FR, V8, P13; GALATIS B, 1974, Biologia Gallo-Hellenica, V5, P259; GIBBS SP, 1970, ANN NY ACAD SCI, V175, P454, DOI 10.1111/j.1749-6632.1970.tb45167.x; GIBBS SP, 1962, J CELL BIOL, V14, P433, DOI 10.1083/jcb.14.3.433; GLOMBITZA KW, 1973, TETRAHEDRON LETT, P4277; GLOMBITZA KW, 1974, PHYTOCHEMISTRY, V13, P1245, DOI 10.1016/0031-9422(74)80110-X; Hellebust J. A., 1974, ALGAL PHYSL BIOCH, P838; JENSEN A, 1952, TIDSSKR KJEMI BERGV, V8, P138; Johansen DA., 1940, PLANT MICROTECHNIQUE; KRISTEN U, 1977, PROTOPLASMA, V92, P243, DOI 10.1007/BF01279461; LACLAIRE JW, 1978, PROTOPLASMA, V97, P93, DOI 10.1007/BF01276686; LAURENT S, 1966, REV GEN BOT, V73, P481; LEPPARD GG, 1973, CAN J BOT, V51, P957, DOI 10.1139/b73-119; LEPPARD GG, 1974, CAN J BOT, V52, P773, DOI 10.1139/b74-100; LOISEAUX S, 1973, J PHYCOL, V9, P277; MAGNE F, 1971, CR ACAD SCI D NAT, V273, P340; MANGENOT G, 1930, B SOC BOT FRANCE, V77, P366; MARTY F, 1978, P NATL ACAD SCI USA, V75, P852, DOI 10.1073/pnas.75.2.852; MARTY F, 1974, THESIS AIX MARSEILLE; MCCULLY ME, 1966, PROTOPLASMA, V62, P287, DOI 10.1007/BF01248267; MCCULLY ME, 1968, J CELL SCI, V3, P1; MOLLENHAUER HH, 1971, J CELL BIOL, V48, P387, DOI 10.1083/jcb.48.2.387; MOLLENHAUER HH, 1978, BOT GAZ, V139, P1, DOI 10.1086/336958; MONTIES B, 1969, B SOC BOT FRANC PHYS, V15, P21; MOSS B, 1967, NEW PHYTOL, V66, P67, DOI 10.1111/j.1469-8137.1967.tb05988.x; MUELLER WC, 1974, PHYSIOL PLANT PATHOL, V4, P187, DOI 10.1016/0048-4059(74)90006-X; MUELLER WC, 1978, J EXP BOT, V29, P757, DOI 10.1093/jxb/29.3.757; MUNDA IVKA, 1962, ACTA ADRIAT, V10, P3; NAGELI C, 1887, NEUEREN ALGENSYSTEME; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; OLIVEIRA L, 1977, NEW PHYTOL, V78, P131, DOI 10.1111/j.1469-8137.1977.tb01551.x; OLIVEIRA L, 1977, J SUBMICR CYTOL PATH, V9, P229; OLIVEIRA L, 1977, PHYCOLOGIA, V16, P235, DOI 10.2216/i0031-8884-16-3-235.1; Papenfuss George F., 1946, BULL TORREY BOT CLUB, V73, P419, DOI 10.2307/2481590; PELLEGRINI L, 1976, PROTOPLASMA, V90, P205, DOI 10.1007/BF01275676; PELLEGRINI L, 1974, CR ACAD SCI D NAT, V279, P903; PELLEGRINI L, 1974, CR ACAD SCI D NAT, V279, P481; PELLEGRINI L, 1978, THESIS AIX MARSEILLE; PERACCHIA C, 1972, J CELL BIOL, V53, P234, DOI 10.1083/jcb.53.1.234; PRAT R, 1977, BIOL CELLULAIRE, V28, P269; RAGAN MA, 1976, BOT MAR, V19, P145, DOI 10.1515/botm.1976.19.3.145; RAGAN MA, 1976, CAN J BIOCHEM CELL B, V54, P66, DOI 10.1139/o76-011; RAMSEY JC, 1976, AM J BOT, V63, P868, DOI 10.2307/2442048; RAWLENCE D J, 1973, Phycologia, V12, P17, DOI 10.2216/i0031-8884-12-1-17.1; ROBB J, 1978, CAN J BOT, V56, P2594, DOI 10.1139/b78-312; ROLAND JC, 1972, STAIN TECHNOL, V47, P195, DOI 10.3109/10520297209116484; THIERY JP, 1967, J MICROSC-PARIS, V6, P987; VAUDOIS B, 1976, B SOC BOT FR, V123, P219, DOI 10.1080/00378941.1976.10835690; VIAN B, 1972, J MICROSC-PARIS, V13, P119; WHATLEY JM, 1977, NEW PHYTOL, V78, P407, DOI 10.1111/j.1469-8137.1977.tb04846.x; WOODING FBP, 1965, J ULTRA MOL STRUCT R, V13, P233, DOI 10.1016/S0022-5320(65)80072-7	95	36	38	0	4	COMPANY BIOLOGISTS LTD	CAMBRIDGE	BIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND	0021-9533	1477-9137		J CELL SCI	J. Cell Sci.		1980	41	FEB					209	231					23	Cell Biology	Cell Biology	JD998	WOS:A1980JD99800016	7364883				2021-04-07	
J	HALL, A				HALL, A			HEAVY-METAL CO-TOLERANCE IN A COPPER-TOLERANT POPULATION OF THE MARINE FOULING ALGA, ECTOCARPUS-SILICULOSUS (DILLW) LYNGBYE	NEW PHYTOLOGIST			English	Article									MANCHESTER POLYTECH,DEPT BIOL SCI,MANCHESTER M1 5GD,ENGLAND							ALLEN WR, 1971, PROC R SOC SER B-BIO, V177, P177, DOI 10.1098/rspb.1971.0022; ANTONOVICS J., 1971, Advances in Ecological Research, V7, P1, DOI 10.1016/S0065-2504(08)60202-0; BELLINGER EG, 1978, ENVIRON POLLUT, V15, P71, DOI 10.1016/0013-9327(78)90062-9; BRADSHAW AD, 1975, P INT C HEAVY METALS, V2, P599; CHATTERJEE BD, 1968, INDIAN J MED RES, V56, P395; COX RM, 1979, NATURE, V279, P231, DOI 10.1038/279231a0; GREGORY RPG, 1965, NEW PHYTOL, V64, P131, DOI 10.1111/j.1469-8137.1965.tb05381.x; HALL A, 1971, MARINE BIOL, V54, P195; HALL A, MARINE BIOL; MORRIS O P, 1973, Marine Pollution Bulletin, V4, P159, DOI 10.1016/0025-326X(73)90256-7; MORRIS OP, 1972, THESIS U LIVERPOOL; NOVICK RP, 1968, J BACTERIOL, V95, P1335, DOI 10.1128/JB.95.4.1335-1342.1968; OKAMOTO K, 1977, AGR BIOL CHEM TOKYO, V41, P17, DOI 10.1080/00021369.1977.10862460; PRESTON J B, 1975, Environmental Health Perspectives, V10, P263; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; STOKES PM, 1973, 8TH P CAN S WAT POLL, P178; Tatsuyama K., 1975, Transactions of the Mycological Society of Japan, V16, P79; Turner R. G., 1969, BRIT ECOL SOC S, V9, P399; TURNER RG, 1967, ISOTOPES PLANT NUTR, P439; VONSTOSCH HA, 1964, 4TH P INT SEAW S, P142; Wainwright S. J., 1975, ECOLOGY RESOURCE DEG, P231; WU L, 1975, NEW PHYTOL, V75, P231, DOI 10.1111/j.1469-8137.1975.tb01391.x; 1952, 580 WOODS HOL OC I U	23	28	29	0	2	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0028-646X			NEW PHYTOL	New Phytol.		1980	85	1					73	78		10.1111/j.1469-8137.1980.tb04449.x			6	Plant Sciences	Plant Sciences	JR894	WOS:A1980JR89400008		Bronze			2021-04-07	
J	HALL, A; FIELDING, AH; BUTLER, M				HALL, A; FIELDING, AH; BUTLER, M			MECHANISMS OF COPPER TOLERANCE IN THE MARINE FOULING ALGA ECTOCARPUS-SILICULOSUS - EVIDENCE FOR AN EXCLUSION MECHANISM	MARINE BIOLOGY			English	Article									MANCHESTER POLYTECH,DEPT BIOL SCI,MANCHESTER M1 5GD,LANCASHIRE,ENGLAND			Butler, Michael/I-8167-2012	Butler, Michael/0000-0002-4907-3652			ANTONOVICS J., 1971, Advances in Ecological Research, V7, P1, DOI 10.1016/S0065-2504(08)60202-0; BAILEY NTJ, 1969, STATISTICAL METHODS; BEPPU M, 1964, J BACTERIOL, V88, P151, DOI 10.1128/JB.88.1.151-157.1964; BOWEN H.J.M, 1966, TRACE ELEMENTS BIOCH; CHOPRA I, 1971, J GEN MICROBIOL, V63, P265; ERNST W, 1976, SEMINAR SERIES SOC E, V1, P115; FERRY JD, 1972, 4TH WOODS HOL OC I B; Fogg GE, 1955, VERH INT VER LIMNOL, V12, P219; FOSTER PL, 1977, NATURE, V269, P322, DOI 10.1038/269322a0; GRYS S, 1976, MIKROCHIM ACTA, V1, P147; MORRIS O P, 1973, Marine Pollution Bulletin, V4, P159, DOI 10.1016/0025-326X(73)90256-7; MORRIS OP, 1972, THESIS U LIVERPOOL; RABANUS A., 1931, Angewandte Botanik, V13, P352; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; RUSSELL P, 1955, NATURE, V176, P1123, DOI 10.1038/1761123b0; SHIMAZONO HIRAO, 1951, JOUR JAPANESE FORESTRY SOC, V33, P393; SILVERBERG BA, 1976, J CELL BIOL, V69, P210, DOI 10.1083/jcb.69.1.210; STOSCH HAV, 1964, P INT SEAWEED S, V4, P142	18	61	63	0	9	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0025-3162			MAR BIOL	Mar. Biol.		1979	54	3					195	199		10.1007/BF00395780			5	Marine & Freshwater Biology	Marine & Freshwater Biology	HT739	WOS:A1979HT73900001					2021-04-07	
J	MULLER, DG				MULLER, DG			GENETIC AFFINITY OF ECTOCARPUS-SILICULOSUS (DILLW) LYNGB FROM THE MEDITERRANEAN, NORTH-ATLANTIC AND AUSTRALIA	PHYCOLOGIA			English	Article										MULLER, DG (corresponding author), UNIV CONSTANCE,FACHBEREICH BIOL,D-7750 CONSTANCE,FED REP GER.						Abbott I. A., 1976, MARINE ALGAE CALIFOR; Davis P. H., 1963, PRINCIPLES ANGIOSPER; FUNK G., 1955, PUBL STAZ ZOOL NAPOL, V25, P1; Harvey W. H., 1838, GENERA S AFRICAN PLA; LINDAUER VW, 1961, NOVA HEDWIGIA, V3, P129; LUND S0REN, 1959, THE MARINE ALGAE OF EAST GREENLAND I TAXONOMICAL PART MEDDEL GRONLAND, V156, P1; LUNING K, 1978, PHYCOLOGIA, V17, P293, DOI 10.2216/i0031-8884-17-3-293.1; MULLER DG, 1976, J PHYCOL, V12, P252, DOI 10.1111/j.0022-3646.1976.00252.x; MULLER DG, 1978, ARCH PROTISTENKD, V120, P371; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1977, BRIT PHYCOL J, V12, P131; MULLER DG, 1976, ARCH MICROBIOL, V1, P89; MUNDA IM, 1978, NOVA HEDWIGIA, V29, P281; POLANSHEK AR, 1975, J PHYCOL, V11, P434, DOI 10.1111/j.0022-3646.1975.00434.x; RAVANKO O, 1970, NOVA HEDWIGIA Z KRYP, V20, P179; Rueness J, 1977, NORSK ALGEFLORA; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; RUSSELL G, 1970, NATURE, V228, P288, DOI 10.1038/228288a0; Skottsberg C., 1907, WISS ERGEBN SCHWED S, V4, P1; SUNDENE O, 1975, Norwegian Journal of Botany, V22, P35; Taylor WR, 1957, MARINE ALGAE NE COAS; WOMERSLEY HB, 1967, AUST J BOT, V15, P189, DOI 10.1071/BT9670189	22	46	46	0	2	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897	0031-8884			PHYCOLOGIA	Phycologia		1979	18	4					312	318		10.2216/i0031-8884-18-4-312.1			7	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	HZ438	WOS:A1979HZ43800002					2021-04-07	
J	MULLER, DG				MULLER, DG			LOCOMOTIVE RESPONSES OF MALE GAMETES TO SPECIES-SPECIFIC SEX ATTRACTANT IN ECTOCARPUS-SILICULOSUS (PHAEOPHYTA)	ARCHIV FUR PROTISTENKUNDE			English	Article										MULLER, DG (corresponding author), UNIV CONSTANCE,FACHBEREICH BIOL,D-7750 CONSTANCE,FED REP GER.							0	30	31	0	1	GUSTAV FISCHER VERLAG	JENA	VILLENGANG 2, D-07745 JENA, GERMANY	0003-9365			ARCH PROTISTENKD	Arch. Protistenkd.		1978	120	4					371	377					7	Microbiology	Microbiology	FV319	WOS:A1978FV31900002					2021-04-07	
J	MULLER, DG				MULLER, DG			SEXUAL REPRODUCTION IN BRITISH ECTOCARPUS-SILICULOSUS (PHAEOPHYTA)	BRITISH PHYCOLOGICAL JOURNAL			English	Article									UNIV CONSTANCE,FACHBEREICH BIOL,D-7750 CONSTANCE,FED REP GER								0	18	19	0	4	ACADEMIC PRESS LTD	LONDON	24-28 OVAL RD, LONDON, ENGLAND NW1 7DX	0007-1617			BRIT PHYCOL J			1977	12	2					131	136					6	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	DP668	WOS:A1977DP66800003					2021-04-07	
J	OLIVEIRA, L; BISALPUTRA, T				OLIVEIRA, L; BISALPUTRA, T			STUDIES IN BROWN ALGA ECTOCARPUS IN CULTURE - CHLOROPLAST	JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY			English	Article									UNIV BRITISH COLUMBIA,DEPT BOT,VANCOUVER V6T 1W5,BC,CANADA							BERKALOFF C, 1961, CR HEBD ACAD SCI, V252, P2747; BISALPUTRA T, 1970, J ULTRA MOL STRUCT R, V32, P417, DOI 10.1016/S0022-5320(70)80019-3; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; BOURNE VL, 1968, CAN J BOTANY, V46, P1369, DOI 10.1139/b68-186; BRACKER CE, 1971, PROTOPLASMA, V73, P15, DOI 10.1007/BF01286408; BROWN R, 1972, PLANT PHYSIOLOGY, V6; CHIHARA M, 1968, Syesis, V1, P87; COLE K, 1968, CAN J GENET CYTOL, V10, P63, DOI 10.1139/g68-009; COLE K, 1968, Syesis, V1, P103; COLE K, 1969, PHYCOLOGIA, V8, P101; CRAN DG, 1973, PROTOPLASMA, V76, P103, DOI 10.1007/BF01279676; CROTTY WJ, 1973, SCIENCE, V182, P839, DOI 10.1126/science.182.4114.839; FRANKE WW, 1971, J CELL BIOL, V51, P881, DOI 10.1083/jcb.51.3.881; FRANKE WW, 1971, PROTOPLASMA, V73, P35, DOI 10.1007/BF01286409; FRANKE WW, 1972, Z ZELLFORSCH MIK ANA, V132, P365, DOI 10.1007/BF02450714; GULLVAG BM, 1968, PHYTOMORPHOLOGY, V18, P520; HARRIS WM, 1970, BOT GAZ, V131, P163, DOI 10.1086/336527; HEITZ E, 1961, Z ZELLFORSCH MIK ANA, V53, P444; JUSTICE C, 1972, HYDROBIOLOGIA, V40, P215, DOI 10.1007/BF00016794; LICHTENTHALER HK, 1968, ENDEAVOUR, V27, P144; MARGULIES MM, 1974, J CELL BIOL, V60, P65, DOI 10.1083/jcb.60.1.65; MARGULIES MM, 1975, BIOCHEM BIOPH RES CO, V64, P735, DOI 10.1016/0006-291X(75)90381-2; MICHAELS A, 1975, BIOCHIM BIOPHYS ACTA, V390, P352, DOI 10.1016/0005-2787(75)90356-1; MORRE DJ, 1971, PROTOPLASMA, V73, P43, DOI 10.1007/BF01286410; NEUSHUL M, 1971, J ULTRA MOL STRUCT R, V37, P532, DOI 10.1016/S0022-5320(71)80023-0; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; OLIVEIRA L, 1975, J SUBMICR CYTOL PATH, V7, P97; OLIVEIRA L, IN PRESS; ORCI L, 1972, J CELL BIOL, V55, P245, DOI 10.1083/jcb.55.1.245; REYNOLDS E, 1973, J CELL BIOL, V17, P208; SCHOTZ F, 1971, BER DEUT BOT GES, V84, P41; STAEHELI.LA, 1967, NATURE, V214, P1158, DOI 10.1038/2141158a0; STAEHELIN A, 1966, Z ZELLFORSCH MIK ANA, V74, P325, DOI 10.1007/BF00401261	33	4	4	0	0	EDITRICE COMPOSITORI BOLOGNA	BOLOGNA	VIA STALINGRADO 97/2, I-40128 BOLOGNA, ITALY	1122-9497			J SUBMICR CYTOL PATH	J. Submicrosc. Cytol. Pathol.		1977	9	2-3					229	237					9	Pathology	Pathology	DP359	WOS:A1977DP35900009					2021-04-07	
J	OLIVEIRA, L; BISALPUTRA, T				OLIVEIRA, L; BISALPUTRA, T			ULTRASTRUCTURAL STUDIES IN BROWN ALGA ECTOCARPUS IN CULTURE - AGING	NEW PHYTOLOGIST			English	Article									UNIV BRITISH COLUMBIA, DEPT BOT, VANCOUVER V6T 1W5, BRITISH COLUMBI, CANADA							AFZELIUS BA, 1955, EXP CELL RES, V8, P147, DOI 10.1016/0014-4827(55)90051-3; ANTIA NJ, 1973, J PROTOZOOL, V20, P377, DOI 10.1111/j.1550-7408.1973.tb00906.x; APARICIO SR, 1969, J CLIN PATHOL, V22, P589, DOI 10.1136/jcp.22.5.589; BARNES BG, 1959, J ULTRA MOL STRUCT R, V3, P131, DOI 10.1016/S0022-5320(59)90010-3; BARTON R, 1966, PLANTA, V71, P314, DOI 10.1007/BF00396319; BERJAK P, 1970, NEW PHYTOL, V69, P929, DOI 10.1111/j.1469-8137.1970.tb02472.x; BERJAK P, 1968, J ULTRA MOL STRUCT R, V23, P233, DOI 10.1016/S0022-5320(68)80003-6; BERJAK P, 1972, NEW PHYTOL, V71, P513, DOI 10.1111/j.1469-8137.1972.tb01951.x; BERJAK P, 1972, NEW PHYTOL, V71, P135, DOI 10.1111/j.1469-8137.1972.tb04820.x; BRIARTY LG, 1970, J EXP BOT, V21, P513, DOI 10.1093/jxb/21.2.513; BRUNK UT, 1972, HISTOCHEM J, V4, P479, DOI 10.1007/BF01011128; BUTLER RD, 1967, J EXP BOT, V18, P535, DOI 10.1093/jxb/18.3.535; Butler RD, 1971, ADV GERONTOL RES, V3, P73; Carr D J, 1967, Symp Soc Exp Biol, V21, P559; DEVECCHI L, 1971, ISRAEL J BOT, V20, P169; DODGE JD, 1970, ANN BOT-LONDON, V34, P817, DOI 10.1093/oxfordjournals.aob.a084412; DRAPER SR, 1971, J EXP BOT, V22, P481, DOI 10.1093/jxb/22.2.481; FABBRI F, 1970, Caryologia, V23, P677; FEDER N, 1968, AM J BOT, V55, P123, DOI 10.1002/j.1537-2197.1968.tb06952.x; FLICKINGER CJ, 1968, J CELL BIOL, V37, P300, DOI 10.1083/jcb.37.2.300; FRANKE WW, 1967, Z ZELLFORSCH MIK ANA, V80, P585, DOI 10.1007/BF00330724; FRANKE WW, 1970, J ULTRA MOL STRUCT R, V30, P317, DOI 10.1016/S0022-5320(70)80065-X; FRITSCH FE, 1945, STRUCTURE REPRODUCTI, V2; GAHAN PB, 1965, J EXP BOT, V16, P350, DOI 10.1093/jxb/16.2.350; GOMEZ M P, 1974, British Phycological Journal, V9, P175; GRASSO JA, 1962, J CELL BIOL, V14, P235, DOI 10.1083/jcb.14.2.235; HARNISCHFEGER G, 1972, PLANTA, V104, P316, DOI 10.1007/BF00386315; IKEDA T, 1964, BOT MAG TOKYO, V77, P336; Jensen W. A., 1962, BOTANICAL HISTOCHEMI; LIPETZ J, 1972, J ULTRA MOL STRUCT R, V39, P43, DOI 10.1016/S0022-5320(72)80005-4; LJUBESIC N, 1968, PROTOPLASMA, V66, P369, DOI 10.1007/BF01252485; LUND HA, 1958, J BIOPHYS BIOCHEM CY, V4, P87, DOI 10.1083/jcb.4.1.87; MATILE P, 1975, CELL BIOLOGY MONOGRA, V1, P27; MATILE P, 1969, PLANT LYSOSOMES LYSO; MCLEAN RJ, 1968, J PHYCOL, V4, P277, DOI 10.1111/j.1529-8817.1968.tb04696.x; MESSER G, 1972, Phycologia, V11, P291, DOI 10.2216/i0031-8884-11-3-291.1; MOOR H, 1963, J CELL BIOL, V17, P609, DOI 10.1083/jcb.17.3.609; OLIVEIRA L, 1977, NEW PHYTOL, V78, P139, DOI 10.1111/j.1469-8137.1977.tb01552.x; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; PALANDRI M, 1972, CARYOLOGIA, V25, P211, DOI 10.1080/00087114.1972.10796478; PALISANO JR, 1972, J PHYCOL, V8, P81, DOI 10.1111/j.1529-8817.1972.tb04005.x; RAWLENCE D J, 1973, Phycologia, V12, P17, DOI 10.2216/i0031-8884-12-1-17.1; ROBARDS AW, 1970, ELECTRON MICROSCOPY; Rockstein M., 1967, P337; ROSENQUIST TH, 1971, STAIN TECHNOL, V46, P253, DOI 10.3109/10520297109067867; SCHUSTER FL, 1968, J PROTOZOOL, V15, P335, DOI 10.1111/j.1550-7408.1968.tb02133.x; SHAW M, 1965, CAN J BOTANY, V43, P747, DOI 10.1139/b65-084; Simon E W, 1967, Symp Soc Exp Biol, V21, P215; SOHAL R S, 1971, Journals of Gerontology, V26, P490; STEARNS ME, 1971, CAN J GENET CYTOL, V13, P550, DOI 10.1139/g71-080; Thung P J, 1967, Symp Soc Exp Biol, V21, P455; VARNER JE, 1961, ANNU REV PLANT PHYS, V12, P245, DOI 10.1146/annurev.pp.12.060161.001333; VILLIERS TA, 1972, NEW PHYTOL, V71, P145, DOI 10.1111/j.1469-8137.1972.tb04821.x; WIENER J, 1965, J CELL BIOL, V27, P107, DOI 10.1083/jcb.27.1.107; Woolhouse H W, 1967, Symp Soc Exp Biol, V21, P179; WUNDERLICH F, 1972, J MICROSC-PARIS, V13, P361; Zeman W, 1971, Adv Gerontol Res, V3, P147	57	10	10	0	1	WILEY	HOBOKEN	111 RIVER ST, HOBOKEN 07030-5774, NJ USA	0028-646X	1469-8137		NEW PHYTOL	New Phytol.		1977	78	1					131	+		10.1111/j.1469-8137.1977.tb01551.x			1	Plant Sciences	Plant Sciences	CT357	WOS:A1977CT35700015		Bronze			2021-04-07	
J	OLIVEIRA, L; BISALPUTRA, T				OLIVEIRA, L; BISALPUTRA, T			ULTRASTRUCTURAL STUDIES IN BROWN ALGA ECTOCARPUS IN CULTURE - AUTOLYSIS	NEW PHYTOLOGIST			English	Article									UNIV BRITISH COLUMBIA,DEPT BOT,VANCOUVER V6T 1W5,BRITISH COLUMBI,CANADA							BAL AK, 1972, Z PFLANZENPHYSIOL, V66, P265, DOI 10.1016/S0044-328X(72)80082-5; BARTON R, 1966, PLANTA, V71, P314, DOI 10.1007/BF00396319; BUTLER RD, 1967, J EXP BOT, V18, P535, DOI 10.1093/jxb/18.3.535; Butler RD, 1971, ADV GERONTOL RES, V3, P73; CLOWES FAL, 1968, PLANT CELLS; CRONSHAW J, 1958, BIOCHIM BIOPHYS ACTA, V27, P89, DOI 10.1016/0006-3002(58)90295-6; DEJONG DW, 1967, SCIENCE, V155, P1672, DOI 10.1126/science.155.3770.1672; DEVECCHI L, 1971, ISRAEL J BOT, V20, P169; DODGE JD, 1970, ANN BOT-LONDON, V34, P817, DOI 10.1093/oxfordjournals.aob.a084412; FABBRI F, 1970, Caryologia, V23, P677; FULCHER RG, 1971, CAN J BOTANY, V49, P161, DOI 10.1139/b71-027; HALPERIN W, 1969, PLANTA, V88, P91, DOI 10.1007/BF01391115; HORTON RF, 1967, NATURE, V214, P1086, DOI 10.1038/2141086a0; IKEDA T, 1964, BOT MAG TOKYO, V77, P336; Lamport DTA., 1965, ADV BOTANICAL RES, V2, P151, DOI [10.1016/S0065-2296(08)60251-7, DOI 10.1016/S0065-2296(08)60251-7]; MCCULLY ME, 1966, PROTOPLASMA, V62, P287, DOI 10.1007/BF01248267; MCCULLY ME, 1968, J CELL SCI, V3, P1; MESSER G, 1972, Phycologia, V11, P291, DOI 10.2216/i0031-8884-11-3-291.1; MYERS A, 1959, PROC R SOC SER B-BIO, V150, P447, DOI 10.1098/rspb.1959.0033; MYERS A, 1959, PROC R SOC SER B-BIO, V150, P456, DOI 10.1098/rspb.1959.0034; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; OLIVEIRA L, 1977, NEW PHYTOL, V78, P131, DOI 10.1111/j.1469-8137.1977.tb01551.x; PERCIVAL E, 1968, Oceanography and Marine Biology an Annual Review, V6, P137; PRESTON RD, 1961, MACROMOLECULAR COMPL, P229; RAWLENCE D J, 1972, Phycologia, V11, P279, DOI 10.2216/i0031-8884-11-3-279.1; SHAW M, 1965, CAN J BOTANY, V43, P747, DOI 10.1139/b65-084; STEARNS ME, 1971, CAN J GENET CYTOL, V13, P550, DOI 10.1139/g71-080; THOMPSON EW, 1969, J EXP BOT, V19, P690	28	4	5	0	4	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0028-646X			NEW PHYTOL	New Phytol.		1977	78	1					139	&		10.1111/j.1469-8137.1977.tb01552.x			0	Plant Sciences	Plant Sciences	CT357	WOS:A1977CT35700016		Bronze			2021-04-07	
J	OLIVEIRA, L; BISALPUTRA, T				OLIVEIRA, L; BISALPUTRA, T			ULTRASTRUCTURAL AND CYTOCHEMICAL STUDIES ON NATURE AND ORIGIN OF CYTOPLASMIC INCLUSIONS OF AGING CELLS OF ECTOCARPUS (PHAEOPHYTA, ECTOCARPALES)	PHYCOLOGIA			English	Article									UNIV BRITISH COLUMBIA,DEPT BOT,VANCOUVER V6T 1W5,BC,CANADA								0	16	17	0	0	INT PHYCOLOGICAL SOC	LAWRENCE	NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897	0031-8884			PHYCOLOGIA	Phycologia		1977	16	3					235	243		10.2216/i0031-8884-16-3-235.1			9	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	DV300	WOS:A1977DV30000003					2021-04-07	
J	MULLER, DG				MULLER, DG			RELATIVE SEXUALITY IN ECTOCARPUS-SILICULOSUS - SCIENTIFIC ERROR	ARCHIVES OF MICROBIOLOGY			English	Article									UNIV KONSTANZ,FACHBEREICH BIOL,D-7750 KONSTANZ,FED REP GER							ESSER K, 1965, GENETIK PILZE; HARTMANN M., 1934, ARCH PROTISTENK, V83, P110; Hartmann M, 1925, NATURWISSENSCHAFTEN, V13, P975, DOI 10.1007/BF01559262; HARTMANN M, 1925, BIOL ZENTRALBL, V45, P449; HARTMANN M, 1956, SEXUALITAT; HARTMANN M, 1909, NATURWISSENSCHAFTEN, V14, P264; HARTMANN M, 1937, ARCH PROTISTENKD, V89, P382; MOEWUS FRANZ, 1939, BIOL ZENTRALBL, V59, P40; MULLER DG, 1968, PLANTA, V81, P160, DOI 10.1007/BF00417445; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1970, NATURWISSENSCHAFTEN, V57, P357; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; Muller DG, 1975, LYNGB ARCH PROTISTEN, V117, P297; RENNER O, 1958, Z NATURFORSCH PT B, V13, P399	14	16	16	0	2	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0302-8933			ARCH MICROBIOL	Arch. Microbiol.		1976	109	1-2					89	94		10.1007/BF00425117			6	Microbiology	Microbiology	CA192	WOS:A1976CA19200013					2021-04-07	
J	OLIVEIRA, L; BISALPUTRA, T				OLIVEIRA, L; BISALPUTRA, T			STUDIES IN BROWN ALGA ECTOCARPUS IN CULTURE - ULTRASTRUCTURAL LOCALIZATION OF ENZYMIC ACTIVITIES	CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE			English	Article									UNIV BRITISH COLUMBIA,DEPT BOT,VANCOUVER V6T 1W5,BRITISH COLUMBI,CANADA							ATKINSON MR, 1967, AUST J BIOL SCI, V20, P1069, DOI 10.1071/BI9671069; BAKER DA, 1973, NEW PHYTOL, V72, P1281, DOI 10.1111/j.1469-8137.1973.tb02104.x; BEARD ME, 1969, J CELL BIOL, V43, pA12; BISALPUTRA T, 1971, J MICROSC-PARIS, V10, P83; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; BOWLING D J F, 1972, Soviet Plant Physiology, V19, P824; BREDEMEIJER GM, 1973, ACTA BOT NEERL, V22, P40, DOI 10.1111/j.1438-8677.1973.tb00903.x; COULOMB MP, 1972, CR ACAD SCI        D, V275, P1035; CRONSHAW J, 1972, J CELL BIOL, V55, pA53; Delincee H, 1970, Biochim Biophys Acta, V200, P404, DOI 10.1016/0005-2795(70)90183-2; EDWARDS ML, 1973, PROTOPLASMA, V78, P321, DOI 10.1007/BF01275700; EILAM Y, 1965, J EXP BOT, V16, P614, DOI 10.1093/jxb/16.4.614; FERGUSON CH, 1973, J EXP BOT, V24, P307, DOI 10.1093/jxb/24.2.307; FISHER J, 1969, PLANT PHYSIOL, V44, P385, DOI 10.1104/pp.44.3.385; FISHER JD, 1970, PLANT PHYSIOL, V46, P812, DOI 10.1104/pp.46.6.812; FREDERICK SE, 1969, J CELL BIOL, V43, P343, DOI 10.1083/jcb.43.2.343; GALSTON AW, 1969, SCIENCE, V163, P1288, DOI 10.1126/science.163.3873.1288; GERHARDT B, 1971, PLANTA, V100, P155, DOI 10.1007/BF00385216; HALL JL, 1972, PLANTA, V108, P103, DOI 10.1007/BF00386073; HALL JL, 1971, ANN BOT-LONDON, V35, P849, DOI 10.1093/oxfordjournals.aob.a084528; HENRIKSON RC, 1971, EXP CELL RES, V68, P456, DOI 10.1016/0014-4827(71)90174-1; HERZOG V, 1972, J CELL BIOL, V55, pA113; HODGES TK, 1972, P NATL ACAD SCI USA, V69, P3307, DOI 10.1073/pnas.69.11.3307; LAI YF, 1972, PHYTOCHEMISTRY, V11, P2747, DOI 10.1016/S0031-9422(00)86507-3; LAI YF, 1972, PLANT PHYSIOL, V50, P452, DOI 10.1104/pp.50.4.452; LEONARD RT, 1972, PLANT PHYSIOL, V49, P436, DOI 10.1104/pp.49.3.436; MAIER K, 1972, PROTOPLASMA, V75, P91, DOI 10.1007/BF01279398; MARGOLIASH E, 1958, BIOCHEM J, V68, P468, DOI 10.1042/bj0680468; NOVIKOFF AB, 1969, J HISTOCHEM CYTOCHEM, V17, P675, DOI 10.1177/17.10.675; NOVIKOFF AB, 1958, J HISTOCHEM CYTOCHEM, V6, P61, DOI 10.1177/6.1.61; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; OLIVEIRA L, TO BE PUBLISHED; POUX N, 1972, J MICROSC-PARIS, V14, P183; Radola B. J., 1970, Brauwissenschaft, V23, P449; RATNER A, 1973, J EXP BOT, V24, P231, DOI 10.1093/jxb/24.1.231; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; RIDGE I, 1971, NATURE-NEW BIOL, V229, P205, DOI 10.1038/newbio229205a0; RIDGE I, 1970, J EXP BOT, V21, P843, DOI 10.1093/jxb/21.4.843; ROELS F, 1973, CR ACAD SCI D NAT, V276, P391; ROTHMAN AH, 1968, EXP PARASITOL, V23, P51, DOI 10.1016/0014-4894(68)90041-6; RUCKER W, 1971, PLANTA, V99, P192, DOI 10.1007/BF00386837; Sacher J A, 1967, Symp Soc Exp Biol, V21, P269; SELIGMAN AM, 1968, J CELL BIOL, V38, P1, DOI 10.1083/jcb.38.1.1; SHANNON LM, 1968, ANN REV PLANT PHYSIO, V19, P187, DOI 10.1146/annurev.pp.19.060168.001155; STRUM JM, 1970, J ULTRA MOL STRUCT R, V31, P323, DOI 10.1016/S0022-5320(70)90135-8; SUNDBERG I, 1973, J PHYCOL, V9, P21; TOLBERT NE, 1971, ANN REV PLANT PHYSIO, V22, P45, DOI 10.1146/annurev.pp.22.060171.000401	47	7	7	0	0	NATL RESEARCH COUNCIL CANADA	OTTAWA	RESEARCH JOURNALS, MONTREAL RD, OTTAWA ON K1A 0R6, CANADA	0008-4026			CAN J BOT	Can. J. Bot.-Rev. Can. Bot.		1976	54	9					913	922		10.1139/b76-095			10	Plant Sciences	Plant Sciences	BU091	WOS:A1976BU09100012					2021-04-07	
J	MULLER, DG				MULLER, DG			SEXUAL ISOLATION BETWEEN A EUROPEAN AND AN AMERICAN POPULATION OF ECTOCARPUS-SILICULOSUS (PHAEOPHYTA)	JOURNAL OF PHYCOLOGY			English	Article									UNIV KONSTANZ,FACHBEREICH BIOL,D-7750 KONSTANZ,FED REP GER							CARDINAL A, 1964, BEIH NOVA HEDWIGIA, V15, P1; JAENICKE L, 1973, LIEBIGS ANN CHEM, P1252; LINDAUER VW, 1961, NOVA HEDWIGIA Z, V3, P143; MULLER D, IN PRESS; MULLER DG, 1975, ARCH PROTISTENK, V177, P297; MULLER DG, 1975, BRIT PHYCOL J, V10, P315; Papenfuss GF, 1934, BOT GAZ, V96, P421; RAVANKO O, 1970, NOVA HEDWIGIA Z KRYP, V20, P179; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144	9	22	22	0	2	PHYCOLOGICAL SOC AMER INC	LAWRENCE	810 EAST 10TH ST, LAWRENCE, KS 66044	0022-3646			J PHYCOL	J. Phycol.		1976	12	2					252	254		10.1111/j.0022-3646.1976.00252.x			3	Plant Sciences; Marine & Freshwater Biology	Plant Sciences; Marine & Freshwater Biology	BX572	WOS:A1976BX57200022					2021-04-07	
J	LOFTHOUSE, PF; CAPON, B				LOFTHOUSE, PF; CAPON, B			ULTRASTRUCTURAL CHANGES ACCOMPANYING MITOSPOROGENESIS IN ECTOCARPUS-PARVUS	PROTOPLASMA			English	Article									CALIF STATE UNIV,DEPT BIOL,LOS ANGELES,CA 90032							BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BROWN RM, 1970, J PHYCOL, V6, P14, DOI 10.1111/j.0022-3646.1970.00014.x; CHI EY, 1971, 7TH P INT SEAW S SAP, P181; COLE K, 1970, Phycologia, V9, P275, DOI 10.2216/i0031-8884-9-3-275.1; FRITSCH FE, 1965, STRUCTURE REPRODUCTI, V2; KUGRENS P, 1972, J PHYCOL           S, V6, P6; LIDDLE LB, 1969, J PHYCOL, V5, P4, DOI 10.1111/j.1529-8817.1969.tb02568.x; OLIVEIRA L, 1973, J SUBMICR CYTOL PATH, V5, P107; Sato T., 1967, J ELECTRONMICROSC, V16, P133; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1	10	15	15	0	1	SPRINGER-VERLAG WIEN	VIENNA	SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA	0033-183X			PROTOPLASMA	Protoplasma		1975	84	1-2					83	99		10.1007/BF02075945			17	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	AD516	WOS:A1975AD51600006	166415				2021-04-07	
J	CLITHEROE, SB; EVANS, LV				CLITHEROE, SB; EVANS, LV			VIRUSLIKE PARTICLES IN BROWN ALGA ECTOCARPUS	JOURNAL OF ULTRASTRUCTURE RESEARCH			English	Article									UNIV LEEDS, DEPT PLANT SCI, LEEDS, ENGLAND							BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BANKS GT, 1969, NATURE, V222, P89, DOI 10.1038/222089b0; BERNHARD W, 1959, CR HEBD ACAD SCI, V249, P483; CHAPMAN RL, 1973, J PHYCOL, V9, P117, DOI 10.1111/j.1529-8817.1973.tb04066.x; DAFT MJ, 1970, NEW PHYTOL, V69, P1029, DOI 10.1111/j.1469-8137.1970.tb02483.x; EVANS LV, 1970, ANN BOT-LONDON, V34, P451, DOI 10.1093/oxfordjournals.aob.a084382; HOWATSON AF, 1960, J BIOPHYS BIOCHEM CY, V7, P753, DOI 10.1083/jcb.7.4.753; LANG NJ, 1968, ANNU REV MICROBIOL, V22, P15, DOI 10.1146/annurev.mi.22.100168.000311; LEE RE, 1971, J CELL SCI, V8, P623; PICKETTHEAPS JD, 1972, J PHYCOL, V8, P44, DOI 10.1111/j.1529-8817.1972.tb03999.x; SAFFERMAN RS, 1963, SCIENCE, V140, P679, DOI 10.1126/science.140.3567.679; SAFFERMAN RS, 1969, VIROLOGY, V39, P775, DOI 10.1016/0042-6822(69)90015-4; SCHNHEPF E, 1970, VIROLOGY, V42, P482, DOI 10.1016/0042-6822(70)90291-6; STOLTZ DB, 1971, J ULTRA MOL STRUCT R, V37, P219, DOI 10.1016/S0022-5320(71)80052-7; STOLTZ DB, 1973, J ULTRA MOL STRUCT R, V43, P58, DOI 10.1016/S0022-5320(73)90070-1; SWALE EMF, 1973, ARCH MIKROBIOL, V92, P91, DOI 10.1007/BF00425007; TIKHONENKO A. S., 1966, MKROBIOLOGYIA, V35, P850; TOTH R, 1972, J PHYCOL, V8, P126, DOI 10.1111/j.1529-8817.1972.tb04011.x; VANZAAYEN A, 1972, VIROLOGY, V47, P94	19	22	22	0	0	ACADEMIC PRESS INC ELSEVIER SCIENCE	SAN DIEGO	525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA	0022-5320			J ULTRA MOL STRUCT R			1974	49	2					211	217		10.1016/S0022-5320(74)80032-8			7	Multidisciplinary Sciences	Science & Technology - Other Topics	U7028	WOS:A1974U702800004	4431075				2021-04-07	
J	MULLER, DG; FALK, H				MULLER, DG; FALK, H			FLAGELLAR STRUCTURE OF GAMETES OF ECTOCARPUS-SILICULOSUS (PHAEOPHYTA) AS REVEALED BY NEGATIVE STAINING	ARCHIV FUR MIKROBIOLOGIE			English	Article									MAX PLANCK INST ZUCHTUNGS FORSCH ABT STRAUB,D-5000 COLOGNE,WEST GERMANY; UNIV FREIBURG,INST BIOL 2,LEHRSTUHL ZELLBIOL,D-7800 FREIBURG,WEST GERMANY							BOUCK GB, 1969, J CELL BIOL, V40, P446, DOI 10.1083/jcb.40.2.446; FAWCETT D, 1961, CELL, V2; GIBBONS IR, 1960, J BIOPHYS BIOCHEM CY, V7, P697, DOI 10.1083/jcb.7.4.697; HOPKINS JM, 1970, J CELL SCI, V7, P823; JAENICKE L, 1973, FORTSCHR CHEM ORG NA; LOISEAUX S, 1970, T AM MICROSC SOC, V89, P524, DOI 10.2307/3224562; MANTON I, 1951, ANN BOT-LONDON, V15, P461, DOI 10.1093/oxfordjournals.aob.a083292; MANTON I., 1964, NEW PHYTQL, V63, P244, DOI 10.1111/j.1469-8137.1964.tb07377.x; Manton I, 1965, ADVANCES BOTANICAL R, V2, P1; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1965, NATURWISSENSCHAFTEN, V52, P311; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815; PETERSEN J. BOYE, 1958, BOT TIDSSKR, V54, P57; SCHUSSNIG B, 1960, HDB PROTOPHYTENKUNDE, V2	14	25	25	0	3	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0003-9276			ARCH MIKROBIOL			1973	91	4					313	322		10.1007/BF00425051			10	Microbiology	Microbiology	Q1219	WOS:A1973Q121900003					2021-04-07	
J	OLIVEIRA, L; BISALPUTRA, T				OLIVEIRA, L; BISALPUTRA, T			STUDIES IN BROWN ALGA ECTOCARPUS IN CULTURE .1. GENERAL ULTRASTRUCTURE OF SPOROPHYTIC VEGETATIVE CELLS	JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY			English	Article									UNIV BRITISH COLUMBIA, DEPT BOT, VANCOUVER 8, BRITISH COLUMBI, CANADA							ARMSTRONG FAJ, 1960, NATURE, V185, P761, DOI 10.1038/185761b0; BAILEY A, 1969, PHYCOLOGIA, V8, P57; BISALPUTRA T, 1969, J ULTRA MOL STRUCT R, V29, P151, DOI 10.1016/S0022-5320(69)80061-4; BISALPUTRA T, 1966, CAN J BOTANY, V44, P89, DOI 10.1139/b66-012; BISALPUTRA T, 1971, J MICROSC-PARIS, V10, P83; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; BOURNE VL, 1968, CAN J BOTANY, V46, P1369, DOI 10.1139/b68-186; CHI EY, 1971, PROTOPLASMA, V72, P101, DOI 10.1007/BF01281014; CHIHARA M, 1968, Syesis, V1, P87; COLE K, 1970, CAN J BOTANY, V48, P265, DOI 10.1139/b70-039; COLE K, 1970, Phycologia, V9, P275, DOI 10.2216/i0031-8884-9-3-275.1; COLE K, 1968, CAN J GENET CYTOL, V10, P63, DOI 10.1139/g68-009; COLE K, 1968, Syesis, V1, P103; COLE K, 1969, PHYCOLOGIA, V8, P101; EVANS LV, 1968, NEW PHYTOL, V67, P173, DOI 10.1111/j.1469-8137.1968.tb05467.x; EVANS LV, 1966, J CELL SCI, V1, P449; FELDMANN G, 1972, CR ACAD SCI D NAT, V275, P751; FOGG GE, 1958, NATURE, V181, P789, DOI 10.1038/181789a0; FRITSCH FE, 1945, STRUCTURE REPRODUCTI, V2; GIBBS SP, 1962, J ULTRA MOL STRUCT R, V7, P247, DOI 10.1016/S0022-5320(62)90021-7; GRIFFITHS DJ, 1970, BOT REV, V36, P29, DOI 10.1007/BF02859154; HORI T, 1971, BOT MAG TOKYO, V84, P231; HORI T, 1972, BOT MAG TOKYO, V85, P125, DOI 10.1007/BF02489509; LAFONTAINE JG, 1963, J CELL BIOL, V17, P167, DOI 10.1083/jcb.17.1.167; LIDDLE LB, 1969, J PHYCOL, V5, P4, DOI 10.1111/j.1529-8817.1969.tb02568.x; LONGEST P, 1946, J E MITCHELL SOC, V62, P248; MAGNE F, 1971, CR ACAD SCI D NAT, V273, P340; MARCHANT R, 1968, ANN BOT-LONDON, V32, P457, DOI 10.1093/oxfordjournals.aob.a084221; MCCULLY ME, 1968, J CELL SCI, V3, P1; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; SCAGEL R.F., 1966, ANNU REV OCEAN MAR B, V4, P123; SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1	32	30	31	0	2	NUOVA IMMAGINE EDITRICE	SIENA	VIA SAN QUIRICO 13, SIENA, 53100, ITALY	1122-9497			J SUBMICR CYTOL PATH	J. Submicrosc. Cytol. Pathol.		1973	5	2					107	120					14	Pathology	Pathology	Q7011	WOS:A1973Q701100004					2021-04-07	
J	BAKER, JRJ; EVANS, LV				BAKER, JRJ; EVANS, LV			SHIP FOULING ALGA ECTOCARPUS .1. ULTRASTRUCTURE AND CYTOCHEMISTRY OF PLURILOCULAR REPRODUCTIVE STAGES	PROTOPLASMA			English	Article									UNIV LEEDS,DEPT PLANT SCI,LEEDS,ENGLAND							BAKER JRJ, 1971, HARV BRIT PHYCOL J, V6, P73; BOUCK GB, 1969, J CELL BIOL, V40, P446, DOI 10.1083/jcb.40.2.446; BOUCK GB, 1965, J CELL BIOL, V26, P523, DOI 10.1083/jcb.26.2.523; COLE K, 1970, Phycologia, V9, P275, DOI 10.2216/i0031-8884-9-3-275.1; EVANS LV, 1970, ANN BOT-LONDON, V34, P451, DOI 10.1093/oxfordjournals.aob.a084382; EVANS LV, 1966, J CELL SCI, V1, P449; EVANS LV, 1973, ALGAL PHYSIOLOGY MET; GIBBS SP, 1962, J ULTRA MOL STRUCT R, V7, P418, DOI 10.1016/S0022-5320(62)90038-2; GIRAUD G, 1962, J MICROSCOPIE, V1, P251; JAMIESON JD, 1967, J CELL BIOL, V34, P577, DOI 10.1083/jcb.34.2.577; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; MANTON I, 1957, J EXP BOT, V8, P294, DOI 10.1093/jxb/8.2.294; MANTON I, 1964, J EXP BOT, V15, P399, DOI 10.1093/jxb/15.2.399; MANTON I., 1964, NEW PHYTQL, V63, P244, DOI 10.1111/j.1469-8137.1964.tb07377.x; RAMBOURG A, 1967, J CELL BIOL, V32, P27, DOI 10.1083/jcb.32.1.27; REYNOLDS ES, 1963, J CELL BIOL, V17, P208, DOI 10.1083/jcb.17.1.208; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; THIERY JP, 1967, J MICROSC-PARIS, V6, P987; THIERY JP, 1970, J MICROSCOPIE, V9, P201	19	62	62	0	3	SPRINGER-VERLAG WIEN	VIENNA	SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA	0033-183X			PROTOPLASMA	Protoplasma		1973	77	1					1	13		10.1007/BF01287289			13	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	P7598	WOS:A1973P759800001					2021-04-07	
J	BAKER, JRJ; EVANS, LV				BAKER, JRJ; EVANS, LV			SHIP-FOULING ALGA ECTOCARPUS .2. ULTRASTRUCTURE OF UNILOCULAR REPRODUCTIVE STAGES	PROTOPLASMA			English	Article									UNIV LEEDS,DEPT PLANT SCI,LEEDS,ENGLAND							BAKER JRJ, 1973, PROTOPLASMA, V77, P1, DOI 10.1007/BF01287289; BRATEN T, 1971, J CELL SCI, V9, P621; EVANS L V, 1970, British Phycological Journal, V5, P1; EVANS LV, 1970, ANN BOT-LONDON, V34, P451, DOI 10.1093/oxfordjournals.aob.a084382; GREENWOOD AD, 1959, J EXP BOT, V10, P55, DOI 10.1093/jxb/10.1.55; HOFFMAN LR, 1963, AM J BOT, V50, P455, DOI 10.2307/2440315; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; MANTON I, 1957, J EXP BOT, V8, P294, DOI 10.1093/jxb/8.2.294; MANTON I., 1964, NEW PHYTQL, V63, P244, DOI 10.1111/j.1469-8137.1964.tb07377.x; MANTON I, 1955, J EXP BOT, V7, P416; MULLER DG, 1966, PLANTA, V68, P57, DOI 10.1007/BF00385371; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; NORTHCOTE DH, 1966, BIOCHEM J, V98, P159, DOI 10.1042/bj0980159; NORTHCOTE DH, 1971, ENDEAVOUR, V30, P26; PAPENFUSS GEORGE F., 1935, BOT GAZ, V96, P421, DOI 10.1086/334493; TAYLOR DL, 1969, J PHYCOL, V5, P336, DOI 10.1111/j.1529-8817.1969.tb02623.x	16	27	28	0	2	SPRINGER-VERLAG WIEN	VIENNA	SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA	0033-183X			PROTOPLASMA	Protoplasma		1973	77	2-3					181	189		10.1007/BF01276756			9	Plant Sciences; Cell Biology	Plant Sciences; Cell Biology	P9807	WOS:A1973P980700002					2021-04-07	
J	MULLER, DG				MULLER, DG			STUDIES ON REPRODUCTION IN ECTOCARPUS-SILICULOSUS	BULLETIN DE LA SOCIETE BOTANIQUE DE FRANCE			English	Article									MAX PLANCK INST ZUCHTUNGS FORSCH,COLOGNE 5,WEST GERMANY							HARTMANN M, 1937, ARCH PROTISTENKD, V89, P382; MULLER DG, 1966, PLANTA, V68, P57, DOI 10.1007/BF00385371; MULLER DG, 1968, PLANTA, V81, P160, DOI 10.1007/BF00417445; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; MULLER DG, 1970, NATURWISSENSCHAFTEN, V57, P357; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DG, 1971, SCIENCE, V171, P815, DOI 10.1126/science.171.3973.815	7	4	4	0	3	SOC BOTANIQUE FRANCE	CHATENAY-MALABRY CEDEX	RUE J B CLEMENT, 92296 CHATENAY-MALABRY CEDEX, FRANCE				B SOC BOT FR			1972							87	97					11	Plant Sciences	Plant Sciences	O3628	WOS:A1972O362800006					2021-04-07	
J	BAKER, JRJ; EVANS, LV				BAKER, JRJ; EVANS, LV			VARIANT MYRIONEMOID OF ECTOCARPUS FASCICULATUS HARV	BULLETIN DE LA SOCIETE BOTANIQUE DE FRANCE			French	Article									UNIV LEEDS,DEPT BOT,LEEDS,YORKSHIRE,ENGLAND							BAKER J, IN PRESS	1	0	0	0	1	SOC BOTANIQUE FRANCE	CHATENAY-MALABRY CEDEX	RUE J B CLEMENT, 92296 CHATENAY-MALABRY CEDEX, FRANCE				B SOC BOT FR			1972							99	100					2	Plant Sciences	Plant Sciences	O3628	WOS:A1972O362800007					2021-04-07	
J	RUSSELL, G; MORRIS, OP				RUSSELL, G; MORRIS, OP			COPPER TOLERANCE IN MARINE FOULING ALGA ECTOCARPUS-SILICULOSUS	NATURE			English	Article																BONEY AD, 1966, BIOLOGY MARINE ALGAE; BRADSHAW AD, 1965, ECOLOGY INDUSTRIAL S, P327; DRING MJ, 1967, J MAR BIOL ASSOC UK, V47, P501, DOI 10.1017/S002531540003513X; PEARY JA, 1964, NATURE, V202, P720, DOI 10.1038/202720a0; RUSSELL G, 1963, J MAR BIOL ASSOC UK, V43, P469, DOI 10.1017/S002531540000045X; SHERIDAN JE, 1968, NEW ZEAL J AGR RES, V11, P601; STOSCH HAV, 1964, 4 C INT ALG MAR BIAR, P142	7	78	78	0	4	MACMILLAN MAGAZINES LTD	LONDON	PORTERS SOUTH, 4 CRINAN ST, LONDON N1 9XW, ENGLAND	0028-0836			NATURE	Nature		1970	228	5268					288	&		10.1038/228288a0			0	Multidisciplinary Sciences	Science & Technology - Other Topics	H5050	WOS:A1970H505000058	5479530				2021-04-07	
J	MULLER, DG				MULLER, DG			INVESTIGATIONS ON NATURE OF A CHEMOTACTIC SUBSTANCE PRODUCED BY FEMALE GAMETES OF ECTOCARPUS SILICULOSUS .I. METHODS ISOLATION AND DETECTION BY GAS CHROMATOGRAPHY	PLANTA			English	Article																COOK AH, 1951, PROC R SOC SER B-BIO, V138, P97, DOI 10.1098/rspb.1951.0008; MULLER DG, 1967, NATURWISSENSCHAFTEN, V54, P496; MULLER DG, 1967, PLANTA, V75, P39, DOI 10.1007/BF00380838; TSUBO Y, 1961, J PROTOZOOL, V8, P114, DOI 10.1111/j.1550-7408.1961.tb01191.x	4	27	27	0	2	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0032-0935			PLANTA	Planta		1968	81	2					160	&		10.1007/BF00417445			0	Plant Sciences	Plant Sciences	B4422	WOS:A1968B442200005	24519651				2021-04-07	
J	RUSSELL, G				RUSSELL, G			GENUS ECTOCARPUS IN BRITAIN .2. FREE-LIVING FORMS	JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM			English	Article																AGARDH JG, 1948, SPECIES GENERA ORDIN, V1; Batters EAL, 1902, J BOT LONDON       S, V40, P1; BURROWS EM, 1958, J MAR BIOL ASSOC UK, V37, P687; Collins F.S., 1914, RHODORA, V16, P1; DETONI JB, 1995, SYLLOGE ALGARUM, V3; FARLOW WJ, 1981, 1879 REP US FISH COM; FOSLIE M, 1894, NORSKE VIDENSK SELSK, P1; GATTY A, 1963, BRITISH SEAWEEDS; GIBB DC, 1957, J ECOL, V45, P49, DOI 10.2307/2257076; GRATTANN WH, 1973, BRITISH MARINE ALGAE; GRAY SO, 1967, BRITISH SEAWEEDS; HARVEY WH, 1946, PHYCOLOGIA BRITANNIC, V1; HARVEY WH, 1949, MANUAL BRITISH MARIN; HARVEY WH, 1941, MANUAL BRITISH ALGAE; HARVEY WH, 1952, NEREIS BOREALIAMER 1; HARVEY WH, 1957, SYNOPSIS BRITISH SEA; HOOKER WJ, 1933, ENGLISH FLORA, V5; JOHNSTONE WG, 1959, NATUREPRINTED BRITIS, V3; KUTZING FT, 1949, SPECIES ALGARUM; LANDSBOROUGH D, 1951, POPULAR HISTORY BRIT; MURRAY G, 1995, INTRODUCTION STUDY S; NEWTON L, 1931, HANDBOOK BRITISH SEA; REINKE J, 1892, BER DT BOT GES, V10, P4; ROSENVINGE LK, 1941, BIOL SKR, V1; RUSSELL G, 1966, J MAR BIOL ASSOC UK, V46, P267, DOI 10.1017/S0025315400027144; SCHILLER J, 1909, INT REV GES HYDROBIO, V2, P62; TAYLOR WR, 1957, MARINE ALGAE NORTHEA; TAYLOR WR, 1937, MARINE ALGAE NORTHEA	28	17	17	0	1	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0025-3154			J MAR BIOL ASSOC UK	J. Mar. Biol. Assoc. U.K.		1967	47	1					233	&		10.1017/S0025315400033695			0	Marine & Freshwater Biology	Marine & Freshwater Biology	90371	WOS:A19679037100019					2021-04-07	
J	MULLER, DG				MULLER, DG			CULTURE EXPERIMENTS ON LIFE CYCLE NUCLEAR PHASES AND SEXUALITY OF BROWN ALGA ECTOCARPUS SILICULOSUS	PLANTA			English	Article																CARAM B, 1964, CR HEBD ACAD SCI, V259, P2495; CARAM B, 1957, CR HEBD ACAD SCI, V245, P440; HARTMANN M., 1934, ARCH PROTISTENK, V83, P110; HARTMANN M, 1937, ARCH PROTISTENKD, V89, P382; LOISEAUX S, 1966, CR ACAD SCI D NAT, V262, P68; MULLER DG, 1966, PLANTA, V68, P57, DOI 10.1007/BF00385371; MULLER DG, 1964, NATURE, V303, P1402; Schreiber E., 1930, PLANTA, V12, P331; Schreiber E, 1935, PLANTA, V24, P266	9	124	125	0	8	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0032-0935			PLANTA	Planta		1967	75	1					39	&		10.1007/BF00380838			0	Plant Sciences	Plant Sciences	93767	WOS:A19679376700006	24550014				2021-04-07	
J	RUSSELL, G				RUSSELL, G			GENUS ECTOCARPUS IN BRITAIN .I. ATTACHED FORMS	JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM			English	Article																BATTERS E.A.L., 1893, GREVILLEA, V21, P85; Batters EAL, 1902, J BOT LONDON       S, V40, P1; Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; CARDINAL A, 1964, BEIH NOVA HEDWIGI 15; CHAPMAN VJ, 1963, B I JAMAICA SCI SER, P1; CHURCH AH, 1920, 10 OXF BOT MEM, P1; Crouan PL, 1867, FLORULE FINISTERE; De Toni JB, 1895, SYLLOGE ALGARUM, V3, pxvi; DILLWYN LW, 1909, BRITISH C; DIXON PETER S., 1964, BOT NOTISER, V117, P279; FOSLIE M, 1891, TROMSO MUSEUMS AARSH, V14, P123; FRITSCH FE, 1945, STRUCTURE REPRODUCTI, V2; Hamel G., 1931, PHEOPHYCEES FRANCE; HOLMES EM, 1890, ANN BOT, V5, P63; JAASUND E, 1965, ACTA U GOTOBURGENSIS; JAASUND ERIK, 1961, BOT NOTISER, V114, P239; JORDE I, 1959, NYTT MAG BOT, V7, P145; KJELLMAN FR, 1872, THESIS STOCKHOLM; KJELLMAN FR, 1890, HANDBOK SKANDINAVIEN; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; KNIGHT M, 1931, P T LPOOL BIOL SOC, V45; KORNMANN P., 1956, PUBBL STAZ ZOOL NAPOLI, V28, P32; KORNMANN P, 1957, HELGOLANDER WISS MEE, V6, P84; KUCKUCK P, 1955, HELGOLANDER WISS MEE, V5, P19; Kuckuck P., 1956, HELGOLANDER WISS MEE, V5, P292; Kuckuck P., 1958, HELGOLANDER WISS MEE, V6, P171; KUTZING FT, 1955, TABULAE PHYCOLOGICAE, V5; KUTZING FT, 1943, PHYCOLOGIA GENERALIS; KYLIN H, 1947, ACTA U LUND, V43; KYLIN H, 1907, THESIS UPSALA; LEJOLIS MA, 1964, MEM SOC SCI NAT CHER, V10, P1; LINDAUER VW, 1961, NOVA HEDWIGIA, V3, P129; LYNGBYE HC, 1919, TENTAMEN HYDROPHYTOP; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; PAPENFUSS GEORGE F., 1935, BOT GAZ, V96, P421, DOI 10.1086/334493; PAPENFUSS GEORGE F., 1933, SCIENCE, V77, P390, DOI 10.1126/science.77.1999.390; PARKE M, 1964, J MAR BIOL ASSOC UK, V44, P499, DOI 10.1017/S0025315400024954; ROSENVINGE LK, 1941, BIOL SKR, V1; RUSSELL G, 1964, J MAR BIOL ASSOC UK, V44, P601, DOI 10.1017/S0025315400027806; SAUVAGEAU C, 1892, J BOT PARIS, V6, P1; Sauvageau C., 1897, ANN SCI NAT BOT BIOL, V5, P161; Sauvageau C, 1900, J BOT PARIS, V14, P213; SCAGEL ROBERT F., 1962, CANADIAN JOUR BOT, V40, P1017; TAYLOR WR, 1957, MARINE ALGAE NORTHEA; WAERN M, 1952, ACTA PHYTOGEOGR SUEC, V30; WOMERSLEY H. B. S., 1964, AUSTRALIAN J BOT, V12, P53, DOI 10.1071/BT9640053	46	41	42	0	0	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0025-3154			J MAR BIOL ASSOC UK	J. Mar. Biol. Assoc. U.K.		1966	46	2					267	&		10.1017/S0025315400027144			0	Marine & Freshwater Biology	Marine & Freshwater Biology	80029	WOS:A19668002900006					2021-04-07	
J	MULLER, DG				MULLER, DG			UNTERSUCHUNGEN ZUR ENTWICKLUNGSGESCHICHTE DER BRAUNALGE ECTOCARPUS SILICULOSUS AUS NEAPEL	PLANTA			English	Article																Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; FOTT B., 1959, ALGENKUNDE; HARTMANN M., 1934, ARCH PROTISTENK, V83, P110; HARTMANN M, 1925, BIOL ZENTRALBL, V45, P449; HARTMANN M, 1937, ARCH PROTISTENKD, V89, P382; KNIGHT M, 1931, T ROY SOC EDINBURGH, V56, P307; KORNMANN P, 1959, HELGOL WISS MEERESUN, V6, P84; MAY VALERIE, 1939, PROC LINN SOC N S WALES, V64, P537; MULLER DG, 1964, NATURE, V203, P1402, DOI 10.1038/2031402a0; MULLER DIETER, 1962, BOT MARINA, V4, P140, DOI 10.1515/botm.1962.4.1-2.140; OLTMANNS F, 1899, FLORA, V86, P86; PAPENFUSS GEORGE F., 1935, BOT GAZ, V96, P421, DOI 10.1086/334493; PAPENFUSS GF, 1951, MANUAL PHYCOLOGY; RUSSELL G, 1962, NATURE, V193, P396, DOI 10.1038/193396a0; SCHUSSNIG BRUNO, 1934, OSTERREICH BOT ZEITSCHR, V83, P81, DOI 10.1007/BF01254357; SMITH GM, 1951, MANUAL PHYCOLOGY	16	18	18	0	2	SPRINGER VERLAG	NEW YORK	175 FIFTH AVE, NEW YORK, NY 10010	0032-0935			PLANTA	Planta		1966	68	1					57	&		10.1007/BF00385371			0	Plant Sciences	Plant Sciences	72156	WOS:A19667215600005	24557722				2021-04-07	
J	BOALCH, GT				BOALCH, GT			STUDIES ON ECTOCARPUS IN CULTURE .1. INTRODUCTION AND METHODS OF OBTAINING UNIALGAL AND BACTERIA-FREE CULTURES	JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM			English	Article																Bold Harold C., 1942, BOT REV, V8, P69, DOI 10.1007/BF02879474; CHU SP, 1946, J MAR BIOL ASSOC UK, V26, P296, DOI 10.1017/S0025315400012145; DROOP MR, 1954, J MAR BIOL ASSOC UK, V33, P511, DOI 10.1017/S002531540000850X; FISH GR, 1950, ACTA HORTI GOTOBURGE, V18, P81; Fogg GE, 1942, J EXP BIOL, V19, P78; FOGG GE, 1951, J EXP BOT, V2, P117, DOI 10.1093/jxb/2.1.117; FOGG GE, 1959, J BIOCHEM MICROBIOL, V1, P59, DOI 10.1002/jbmte.390010107; Hamel G., 1931, PHEOPHYCEES FRANCE; HARRIS JE, 1943, J IRON ST INST, V148, P405; HARVEY H. W., 1941, JOUR MARINE BIOL ASSOC UNITED KINGDOM, V25, P225; KAIN JM, 1960, J MAR BIOL ASSOC UK, V39, P33, DOI 10.1017/S0025315400013084; KAIN JM, 1958, J MAR BIOL ASSOC UK, V37, P397, DOI 10.1017/S0025315400023778; KYLIN H, 1947, ACTA U LUND, V43, P1; KYLIN H, 1943, BOT NOTISER, P295; NEWTON L, 1931, HANDBOOK BRITISH SEA; PAPENFUSS GEORGE F., 1935, BOT GAZ, V96, P421, DOI 10.1086/334493; Pringsheim EG, 1946, PURE CULTURES ALGAE; PROVASOLI L, 1957, ARCH MIKROBIOL, V25, P392, DOI 10.1007/BF00446694; PROVASOLI LUIGI, 1951, PROC AMER SOC PROTOZOOL, V2, P6; REDFORD EL, 1951, J CELL COMPAR PHYSL, V38, P217, DOI 10.1002/jcp.1030380208; ROSENVINGE LK, 1941, BIOL SKR, V1, P1; Schramm JR, 1914, ANN MO BOT GARD, V1, P23; SPENCER CP, 1952, J MAR BIOL ASSOC UK, V31, P97, DOI 10.1017/S0025315400003714; ZOBELL CE, 1946, MARINE MICROBIOLOGY	24	19	19	0	3	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0025-3154			J MAR BIOL ASSOC UK	J. Mar. Biol. Assoc. U.K.		1961	41	1					279	&		10.1017/S0025315400023900			0	Marine & Freshwater Biology	Marine & Freshwater Biology	5607B	WOS:A19615607B00009					2021-04-07	
J	BOALCH, GT				BOALCH, GT			STUDIES ON ECTOCARPUS IN CULTURE .2. GROWTH AND NUTRITION OF A BACTERIA-FREE CULTURE	JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM			English	Article																BIEBL R, 1952, J MAR BIOL ASSOC UK, V31, P307, DOI 10.1017/S0025315400053017; BLACK WAP, 1948, J SOC CHEM IND-L, V67, P165, DOI 10.1002/jctb.5000670411; BOALCH GT, 1961, J MAR BIOL ASSOC UK, V41, P279, DOI 10.1017/S0025315400023900; BONEY AD, 1960, BRIT PHYCOL B, V2, P38; COOPER L. H. N., 1937, JOUR MARINE BIOL ASSOC UNITED KINGDOM, V22, P177; DROOP MR, 1955, J MAR BIOL ASSOC UK, V34, P233, DOI 10.1017/S0025315400027612; DROOP MR, 1958, J MAR BIOL ASSOC UK, V37, P323, DOI 10.1017/S0025315400023729; DROOP MR, 1957, NATURE, V180, P1041, DOI 10.1038/1801041b0; DROOP MR, 1957, J GEN MICROBIOL, V16, P286, DOI 10.1099/00221287-16-1-286; Fogg G. E., 1944, NEW PHYTOL, V43, P164, DOI 10.1111/j.1469-8137.1944.tb05012.x; FOGG GE, 1958, NATURE, V181, P789, DOI 10.1038/181789a0; FOGG GE, 1959, J BIOCHEM MICROBIOL, V1, P59, DOI 10.1002/jbmte.390010107; Fogg GE, 1953, METABOLISM ALGAE; GOLDBERG ED, 1952, BIOL BULL, V102, P243, DOI 10.2307/1538372; HARVEY H. W., 1939, JOUR MARINE BIOL ASSOC UNITED KINGDOM, V23, P499; HARVEY H. W., 1937, JOUR MARINE BIOL ASSOC UNITED KINGDOM, V22, P205; HARVEY HW, 1947, J MAR BIOL ASSOC UK, V26, P562, DOI 10.1017/S002531540001376X; HARVEY HW, 1955, CHEMISTRY FERTILITY; Hyde MB, 1938, J ECOL, V26, P118, DOI 10.2307/2256415; KAIN JM, 1958, J MAR BIOL ASSOC UK, V37, P781, DOI 10.1017/S0025315400005774; KAIN JM, 1938, J MAR BIOL ASS UK, V37, P397; MILNER HW, 1953, PUBL CARNEG INST 600, P285; MYERS J, 1953, PUBL CARNEG INST 600, P37; Pearsall WH, 1937, PROC R SOC SER B-BIO, V121, P451; Pringsheim EG, 1951, MANUAL PHYCOLOGY, P347; Pringsheim EG, 1946, PURE CULTURES ALGAE; PROVASOLI L, 1957, ARCH MIKROBIOL, V25, P392, DOI 10.1007/BF00446694; Redfield A. C., 1934, P176; RITCHIE D, 1957, AM J BOT, V44, P870, DOI 10.2307/2438907; RYTHER JH, 1954, BIOL BULL, V106, P198, DOI 10.2307/1538713; SPENCER CP, 1954, J MAR BIOL ASSOC UK, V33, P265, DOI 10.1017/S0025315400003593; WHITAKER D. M., 1937, BIOL BULL, V73, P552, DOI 10.2307/1537614	32	32	32	0	2	CAMBRIDGE UNIV PRESS	NEW YORK	40 WEST 20TH STREET, NEW YORK, NY 10011-4211	0025-3154			J MAR BIOL ASSOC UK	J. Mar. Biol. Assoc. U.K.		1961	41	1					287	&		10.1017/S0025315400023912			0	Marine & Freshwater Biology	Marine & Freshwater Biology	5607B	WOS:A19615607B00010					2021-04-07	
J	Papenfuss, GF				Papenfuss, GF			Alternation of generations in Ectocarpus siliculosus	BOTANICAL GAZETTE			English	Article									Johns Hopkins Univ, Bot Lab, Baltimore, MD USA							Baumgartner WJ, 1932, STAIN TECHNOL, V7, P129, DOI 10.3109/10520293209116087; Berthold G., 1881, MITT ZOOL STAT NEAPE, V2, P401; CHAMBERLAIN CHARLES JOSEPH, 1928, PUBL PUGET SOUND BIOL STA, V5, P319; DAMMANN HILDEGARD, 1930, WISS MEERESUNTERSUCH ABT HEL GOLAND, V18, P1; HARTMANN M, 1925, BIOL ZENTRALBL, V45, P449; KNIGHT M, 1929, T ROY SOC EDINBURGH, V56, P307; KNIGHT M, 1923, T ROY SOC EDINBURGH, V53, P343; Krause R, 1927, ENZYKLOPADIE MIKROSK; KUCKUCK P, 1897, WISS MEERESUNTERS AB, V2, P35; KUCKUCK P, 1891, BOT CENTRALBLATT, V48, P97; KUCKUCK P, 1898, BER DEUT BOT GES, V16, P35; KUCKUCK P, 1891, BOT CENTRALBLATT, V48, P33; KUCKUCK P, 1891, BOT CENTRALBLATT, V48, P1; KUCKUCK P, 1891, BOT CENTRALBLATT, V48, P65; KUCKUCK P, 1912, WISS MEERESUNTERS AB, V5, P115; KUCKUCK P, 1894, BIOL ANST HELGOLAND, V1, P223; KUCKUCK P, 1899, WISS MEERESUNTERS AB, V3, P45; KUCKUCK P, 1897, BIOL ANSTALT HELGOLA, V2, P371; KUCKUCK P, 1891, BOT CENTRALBLATT, V48, P129; KYLIN H, 1918, SVENSK BOT TIDSKR, V12, P1; KYLIN H, 1933, UBER ENTWICKLUNGSGES, V29, P1; LEE B, 1928, MICROTOMIST VADE MEC; Margolena LA, 1932, STAIN TECHNOL, V7, P9; McClung Clarence E., 1929, HDB MICROSCOPICAL TE; OLTMANNS F, 1899, FLORA, V86, P86; PAPENFUSS GEORGE F., 1933, SCIENCE, V77, P390, DOI 10.1126/science.77.1999.390; REINHARDT L, 1884, BOT CENTRALBL, V18, P126; SAUVAGEAU C, 1896, J BOT, V10, P357; SAUVAGEAU C, 1897, J BOT, V11, P24; SAUVAGEAU C, 1897, J BOT, V11, P66; SAUVAGEAU C., 1896, J BOT, V10, P388; SAUVAGEAU C, 1897, J BOT, V11, P5; SAUVAGEAU C, 1897, MEM SOC SCI NAT CHER, V30, P293; SAUVAGEAU C, 1896, CR HEBD ACAD SCI, V123, P431; SAUVAGEAU C, 1896, ANN SCI NAT BOT BIOL, V8, P223; Schreiber E., 1930, PLANTA, V12, P331; Styer JF, 1930, AM J BOT, V17, P636, DOI 10.2307/2435663; Waksman SA, 1926, J BACTERIOL, V12, P87, DOI 10.1128/JB.12.2.87-95.1926; Westbrook MA, 1930, ANN BOT-LONDON, V44, P1011, DOI 10.1093/oxfordjournals.aob.a090248	39	6	6	0	1	UNIV CHICAGO PRESS	CHICAGO	1427 E 60TH ST, CHICAGO, IL 60637-2954 USA	0006-8071			BOT GAZ	Bot. Gaz.		1934	96						421	U22					29	Plant Sciences	Plant Sciences	V31YT	WOS:000202159900025					2021-04-07	
J	Sauvageau, C				Sauvageau, C			On four Ectocarpus	COMPTES RENDUS HEBDOMADAIRES DES SEANCES DE L ACADEMIE DES SCIENCES			French	Article																Mathias E., 1932, Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences, V194, P413; 1931, COMPTES RENDUS, V193, P971	2	1	1	0	0	GAUTHIER-VILLARS/EDITIONS ELSEVIER	PARIS	23 RUE LINOIS, 75015 PARIS, FRANCE	0001-4036			CR HEBD ACAD SCI	C. R. Hebd. Seances Acad. Sci.	APR-JUN	1932	194						2260	2261					2	Multidisciplinary Sciences	Science & Technology - Other Topics	V13PQ	WOS:000200919400453					2021-04-07	
J	Sauvageau, C				Sauvageau, C			On the third type of plurilocular organs of Ectocarpus secundus Kutz.	COMPTES RENDUS HEBDOMADAIRES DES SEANCES DE L ACADEMIE DES SCIENCES			French	Article																1896, COMPTES RENDUS, V123, P360; 1896, J BOT, V10, P11	2	0	0	0	0	GAUTHIER-VILLARS/EDITIONS ELSEVIER	PARIS	23 RUE LINOIS, 75015 PARIS, FRANCE	0001-4036			CR HEBD ACAD SCI	C. R. Hebd. Seances Acad. Sci.		1931	193						971	972					2	Multidisciplinary Sciences	Science & Technology - Other Topics	V13WF	WOS:000200936500400					2021-04-07	
J	Hartmann, M				Hartmann, M			Relative sexuality of ectocarpus siliculosis - An experimental article on sexuality hypothesis of fertilisation	NATURWISSENSCHAFTEN			German	Article																Belar K., 1924, Archiv fuer Protistenkunde Jena, V48, P371; BELAR K, 1921, ARCH PROTISTENKD, V46, P1; BERTHOLD G, 1881, MITT ZOOL STAT NEAPE, V2; BERTHOLD G, 1897, FLORA, V83, P419; BLAKESLEE AF, 1904, P MAT ACAD SCI USA, V10; BRUNSWIK H, 1924, UNTERSUCHUNGEN GESCH; BURGEFF H, 1914, FLORA, V107, P108; CORRENS C, 1913, HANDWORTERBUCH NATUR, V4; GOETSCH W, 1923, BIOL ZENTRALBL, V43; GOETSCH W, 1922, BIOL ZENTRALBL, V42; GOETSCH W, 1921, BIOL ZENTRALBL, V41; HAMMERLING J, 1924, ZOOL JB, V41; HARTMANN M, 1923, STUDIA MENDELIANA; HARTMANN M, 1917, NATURWISSENSCHAFTEN, V6; HARTMANN M, 1921, ARCH PROTISTENKUNDE, V43; HARTMANN M, 1909, ARCH PROTISTENKUNDE, V14; KNIEP H, 1919, VERHANDL PHYS MED GE; OLTMANNS F, 1899, FLORA, V85, P1; Oltmanns F., 1922, MORPHOLOGIE BIOL ALG, VII; Schreiber E., 1925, Zeitschr Bot Jena, V17, P337	20	6	6	0	1	SPRINGER	NEW YORK	233 SPRING STREET, NEW YORK, NY 10013 USA	0028-1042			NATURWISSENSCHAFTEN	Naturwissenschaften	FEB	1925	13			1/2			975	980		10.1007/BF01559262			6	Multidisciplinary Sciences	Science & Technology - Other Topics	V03XD	WOS:000200262900165					2021-04-07	
J	Sauvageau, C				Sauvageau, C			New observations on the Ectocarpus Padin ae Sauv.	COMPTES RENDUS HEBDOMADAIRES DES SEANCES DE L ACADEMIE DES SCIENCES			French	Article																SAUVAGEAU C, 1896, J BOT, V10; SAUVAGEAU C, 1899, J BOT, V13; SAUVAGEAU C, 1895, J BOT, V9; SAUVAGEAU C, 1897, J BOT, V11	4	1	1	0	0	GAUTHIER-VILLARS/EDITIONS ELSEVIER	PARIS	23 RUE LINOIS, 75015 PARIS, FRANCE	0001-4036			CR HEBD ACAD SCI	C. R. Hebd. Seances Acad. Sci.		1920	171						1041	1044					4	Multidisciplinary Sciences	Science & Technology - Other Topics	V14AK	WOS:000200947400389					2021-04-07	
